LOCUS       pJAB2495_proG1090::BetI 5751 bp ds-DNA     circular     30-JUN-2022
DEFINITION  .
FEATURES             Location/Qualifiers
     misc_feature    1..25
                     /label="T-Border (left)"
                     /ApEinfo_revcolor=#808000
                     /ApEinfo_fwdcolor=#808000
     terminator      complement(76..359)
                     /label="NOS\terminator"
                     /ApEinfo_revcolor=#d59687
                     /ApEinfo_fwdcolor=#d59687
     misc_marker     complement(367..918)
                     /label="BAR"
                     /ApEinfo_revcolor=#84b0dc
                     /ApEinfo_fwdcolor=#84b0dc
     misc_feature    complement(640..640)
                     /label="G->T mutation to remove Bbs1 site"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     promoter        complement(935..1227)
                     /label="pNOS"
                     /ApEinfo_revcolor=#84b0dc
                     /ApEinfo_fwdcolor=#84b0dc
     misc_feature    1265..1270
                     /label="Bbs1"
                     /ApEinfo_revcolor=#ff0000
                     /ApEinfo_fwdcolor=#ff0000
     misc_feature    1273..1276
                     /label="Bsa1 seam"
                     /ApEinfo_revcolor=#f8d3a9
                     /ApEinfo_fwdcolor=#f8d3a9
     misc_feature    1279..1282
                     /label="Bsa1 seam"
                     /ApEinfo_revcolor=#f8d3a9
                     /ApEinfo_fwdcolor=#f8d3a9
     misc_feature    1283..1525
                     /label="G10-90 promoter"
                     /ApEinfo_revcolor=#84b0dc
                     /ApEinfo_fwdcolor=#84b0dc
     misc_feature    1526..1529
                     /label="Bsa1 seam"
                     /ApEinfo_revcolor=#f8d3a9
                     /ApEinfo_fwdcolor=#f8d3a9
     misc_feature    1530..2114
                     /label="BetI"
                     /ApEinfo_revcolor=#0000ff
                     /ApEinfo_fwdcolor=#0000ff
     misc_feature    2115..2123
                     /label="Gly-Gly-Gly linker"
                     /ApEinfo_revcolor=#5f76b8
                     /ApEinfo_fwdcolor=#5f76b8
     misc_feature    2124..2357
                     /label="VP16 Hasseloff"
                     /ApEinfo_revcolor=#6e42fa
                     /ApEinfo_fwdcolor=#6e42fa
     misc_feature    2358..2378
                     /label="SV40 NLS"
                     /ApEinfo_revcolor=#84b0dc
                     /ApEinfo_fwdcolor=#84b0dc
     misc_feature    2379..2381
                     /label="Activator stop codon"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     misc_feature    2382..2385
                     /label="Bsa1 seam"
                     /ApEinfo_revcolor=#f8d3a9
                     /ApEinfo_fwdcolor=#f8d3a9
     terminator      2389..2613
                     /label="19STer"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     misc_feature    2614..2617
                     /label="Bsa1 seam"
                     /ApEinfo_revcolor=#f8d3a9
                     /ApEinfo_fwdcolor=#f8d3a9
     misc_feature    2620..2623
                     /label="Bsa1 seam"
                     /ApEinfo_revcolor=#f8d3a9
                     /ApEinfo_fwdcolor=#f8d3a9
     misc_feature    complement(2626..2631)
                     /label="Bbs1"
                     /ApEinfo_revcolor=#ff0000
                     /ApEinfo_fwdcolor=#ff0000
     primer_bind     complement(2690..2709)
                     /label="T3"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     primer_bind     complement(2727..2747)
                     /label="M13-rev"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     misc_feature    3084..3132
                     /label="RB right border"
                     /ApEinfo_revcolor=#b1ff67
                     /ApEinfo_fwdcolor=#b1ff67
     misc_feature    3181..3862
                     /label="colE1"
                     /ApEinfo_revcolor=#7f7f7f
                     /ApEinfo_fwdcolor=#7f7f7f
     misc_marker     complement(3881..4666)
                     /label="Spec-R"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     misc_feature    complement(4667..4878)
                     /label="spec promoter"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     misc_feature    5119..5119
                     /label="C->A mutation"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     misc_feature    5120..5672
                     /label="pSa ori"
                     /ApEinfo_revcolor=#84b0dc
                     /ApEinfo_fwdcolor=#84b0dc
ORIGIN
        1 tggcaggata tattgtggtg taaacaaatt gacgcttaga caacttaata acacattgcg
       61 gacgttttta atgtaatcag cttgcatgcc ggtcgatcta gtaacataga tgacaccgcg
      121 cgcgataatt tatcctagtt tgcgcgctat attttgtttt ctatcgcgta ttaaatgtat
      181 aattgcggga ctctaatcat aaaaacccat ctcataaata acgtcatgca ttacatgtta
      241 attattacat gcttaacgta attcaacaga aattatatga taatcatcgc aagaccggca
      301 acaggattca atcttaagaa actttattgc caaatgtttg aacgatctgc ttgactctag
      361 gggtcatcag atttcggtga cgggcaggac cggacggggc ggcaccggca ggctgaagtc
      421 cagctgccag aaacccacgt catgccagtt cccgtgcttg aagccggccg cccgcagcat
      481 gccgcggggg gcatatccga gcgcctcgtg catgcgcacg ctcgggtcgt tgggcagccc
      541 gatgacagcg accacgctct tgaagccctg tgcctccagg gacttcagca ggtgggtgta
      601 gagcgtggag cccagtcccg tccgctggtg gcggggggaC acgtacacgg tcgactcggc
      661 cgtccagtcg taggcgttgc gtgccttcca gggacccgcg taggcgatgc cggcgacctc
      721 gccgtccacc tcggcgacga gccagggata gcgctcccgc agacggacga ggtcgtccgt
      781 ccactcctgc ggttcctgcg gctcggtacg gaagttgacc gtgcttgtct cgatgtagtg
      841 gttgacgatg gtgcagaccg ccggcatgtc cgcctcggtg gcacggcgga tgtcggccgg
      901 gcgtcgttct gggctcatgg tagatcccct cgatcgagtt gagagtgaat atgagactct
      961 aattggatac cgaggggaat ttatggaacg tcagtggagc atttttgaca agaaatattt
     1021 gctagctgat agtgacctta ggcgactttt gaacgcgcaa taatggtttc tgacgtatgt
     1081 gcttagctca ttaaactcca gaaacccgcg gctcagtggc tccttcaacg ttgcggttct
     1141 gtcagttcca aacgtaaaac ggcttgtccc gcgtcatcgg cgggggtcat aacgtgactc
     1201 ccttaattct ccgctcatga tcgataacat gtcgactatc ggacgagctc aaagtttaac
     1261 gTgtGAAGAC gtgcttctaa tgtagtttaa actgaaggcg ggaaacgaca atctgatcca
     1321 agctcaagct aagcttgcat gcctgcagga tatcgtggat ccaagcttgc cacgtgccgc
     1381 cacgtgccgc cacgtgccgc cacgtgcctc tagaggatcc atctccactg acgtaaggga
     1441 tgacgcacaa tcccactatc cttcgcaaga cccttcctct atataaggaa gttcatttca
     1501 tttggagagg acacgctggg atcCccaccA TGCCGAAACT GGGTATGCAG AGCATTCGTC
     1561 GTCGTCAGCT GATTGATGCA ACCCTGGAAG CAATTAATGA AGTTGGTATG CATGATGCAA
     1621 CCATTGCACA GATTGCACGT CGTGCCGGTG TTAGCACCGG TATTATTAGC CATTATTTCC
     1681 GCGATAAAAA CGGTCTGCTG GAAGCAACCA TGCGTGATAT TACCAGCCAG CTGCGTGATG
     1741 CAGTTCTGAA TCGTCTGCAT GCACTGCCGC AGGGTAGCGC AGAACAGCGT CTGCAGGCAA
     1801 TTGTTGGTGG TAATTTTGAT GAAACCCAGG TTAGCAGCGC AGCAATGAAA GCATGGCTGG
     1861 CATTTTGGGC AAGCAGCATG CATCAGCCGA TGCTGTATCG TCTGCAGCAG GTTAGCAGTC
     1921 GTCGTCTGCT GAGCAATCTG GTTAGCGAAT TTCGTCGTGA ACTGCCTCGT GAACAGGCAC
     1981 AAGAGGCAGG TTATGGTCTG GCAGCACTGA TTGATGGTCT GTGGCTGCGT GCAGCACTGA
     2041 GCGGTAAACC GCTGGATAAA ACCCGTGCAA ATAGCCTGAC CCGTCATTTT ATCACCCAGC
     2101 ATCTGCCGAC CGATGGAGGA GGTGCCCCCC CGACCGACGT CAGCCTGGGG GACGAGCTCC
     2161 ACTTAGACGG CGAGGACGTG GCGATGGCGC ATGCCGACGC GCTAGACGAT TTCGATCTGG
     2221 ACATGTTGGG GGACGGGGAT TCCCCGGGGC CGGGATTTAC CCCCCACGAC TCCGCCCCCT
     2281 ACGGCGCTCT GGATACGGCC GACTTCGAGT TTGAGCAGAT GTTTACCGAT GCCCTTGGAA
     2341 TTGACGAGTA CGGTGGGccg aagaagaaga ggaaggttTA Gtgtcggtac gctgaaatca
     2401 ccagtctctc tctacaaatc tatctctctc tattttctcc ataaataatg tgtgagtagt
     2461 ttcccgataa gggaaattag ggttcttata gggtttcgct catgtgttga gcatataaga
     2521 aacccttagt atgtatttgt atttgtaaaa tacttctatc aataaaattt ctaattccta
     2581 aaaccaaaat ccagtactaa aatccagatc gatgcttgat actacGTCTT Cgggctagag
     2641 cggactagtt ctagagcggc cgccaccgcg gtggagctcc agcttttgtt ccctttagtg
     2701 agggttaatt gcgcgcttgg cgtaatcatg gtcatagctg tttcctgtgt gaaattgtta
     2761 tccgctcaca attccacaca acatacgagc cggaagcata aagtgtaaag cctggggtgc
     2821 ctaatgagtg agctaactca cattaattgc gttgcgctca ctgcccgctt tccagtcggg
     2881 aaacctgtcg tgccagctgc attaatgaat cggccaacgc gcggggagag gcggtttgcg
     2941 tattgggcgc tcttccgctt cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg
     3001 gcgagcggta tcagctcact caaaggcggt aatacggtta tccacagaat caggggataa
     3061 cgcaggaaag aacatgaagg ccttgacagg atatattggc gggtaaacta agtcgctgta
     3121 tgtgtttgtt tgagatctca tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa
     3181 ggccgcgttg ctggcgtttt tccataggct ccgcccccct gacgagcatc acaaaaatcg
     3241 acgctcaagt cagaggtggc gaaacccgac aggactataa agataccagg cgtttccccc
     3301 tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat acctgtccgc
     3361 ctttctccct tcgggaagcg tggcgctttc tcatagctca cgctgtaggt atctcagttc
     3421 ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa ccccccgttc agcccgaccg
     3481 ctgcgcctta tccggtaact atcgtcttga gtccaacccg gtaagacacg acttatcgcc
     3541 actggcagca gccactggta acaggattag cagagcgagg tatgtaggcg gtgctacaga
     3601 gttcttgaag tggtggccta actacggcta cactagaaga acagtatttg gtatctgTgc
     3661 tctgctgaag ccagttacct tcggaagaag agttggtagc tcttgatccg gcaaacaaac
     3721 caccgctggt agcggtggtt tttttgtttg caagcagcag attacgcgca gaaaaaaagg
     3781 atctcaagaa gatcctttga tcttttctac ggggtctgac gctcagtgga acgaaaactc
     3841 acgttaaggg attttggtca tgaattcttg ttagacatta tttgccgact accttggtga
     3901 tctcgccttt cacgtagtga acaaattctt ccaactgatc tgcgcgcgag gccaagcgat
     3961 cttcttgtcc aagataagcc tgcctagctt caagtatgac gggctgatac tgggccggca
     4021 ggcgctccat tgcccagtcg gcagcgacat ccttcggcgc gattttgccg gttactgcgc
     4081 tgtaccaaat gcgggacaac gtaagcacta catttcgctc atcgccagcc cagtcgggcg
     4141 gcgagttcca tagcgttaag gtttcattta gcgcctcaaa tagatcctgt tcaggaaccg
     4201 gatcaaagag ttcctccgcc gctggaccta ccaaggcaac gctatgttct cttgcttttg
     4261 tcagcaagat agccagatca atgtcgatcg tggctggctc gaagatacct gcaagaatgt
     4321 cattgcgctg ccattctcca aattgcagtt cgcgcttagc tggataacgc cacggaatga
     4381 tgtcgtcgtg cacaacaatg gtgacttcta cagcgcggag aatctcgctc tctccagggg
     4441 aagccgaagt ttccaaaagg tcgttgatca aagctcgccg cgttgtttca tcaagcctta
     4501 cggtcaccgt aaccagcaaa tcaatatcac tgtgtggctt caggccgcca tccactgcgg
     4561 agccgtacaa atgtacggcc agcaacgtcg gttcgagatg gcgctcgatg acgccaacta
     4621 cctctgatag ttgagtcgat acttcggcga tcaccgcttc cctcatgatg tttaactcct
     4681 gaattaagcc gcgccgcgaa gcggtgtCgg cttgaatgaa ttgttaggcg tcatcctgtg
     4741 ctcccgagaa ccagtaccag tacatcgctg tttcgttcga gacttgaggt ctagttttat
     4801 acgtgaacag gtcaatgccg ccgagagtaa agccacaTtt tgcgtacaaa ttgcaggcag
     4861 gtacattgtt cgtttgtgCC ATGATGATAT ATTTTTATCT TGTGCAATGT AACATCAGAG
     4921 ATTTTGAGAC ACAACGTGGC TTTGTTGAAT AAATCGAACT TTTGCTGAGT TGAAGGATCA
     4981 GATCACGCAT CTTCCCGACA ACGCAGACCG TTCCGTGGCA AAGCAAAAGT TCAAAATCAC
     5041 CAACTGGTCC ACCTACAACA AAGCTCTCAT CAACCGTGGC TCCCTCACTT TCTGGCTGGA
     5101 TGATGGGGCG ATTCAGGCAG ATCCCCATCC AACAGCCCGC CGTCGAGCGG GCTTTTTTAT
     5161 CCCCGGAAGC CTGTGGATAG AGGGTAGTTA TCCACGTGAA ACCGCTAATG CCCCGCAAAG
     5221 CCTTGATTCA CGGGGCTTTC CGGCCCGCTC CAAAAACTAT CCACGTGAAA TCGCTAATCA
     5281 GGGTACGTGA AATCGCTAAT CGGAGTACGT GAAATCGCTA ATAAGGTCAC GTGAAATCGC
     5341 TAATCAAAAA GGCACGTGAG AACGCTAATA GCCCTTTCAG ATCAACAGCT TGCAAACACC
     5401 CCTCGCTCCG GCAAGTAGTT ACAGCAAGTA GTATGTTCAA TTAGCTTTTC AATTATGAAT
     5461 ATATATATCA ATTATTGGTC GCCCTTGGCT TGTGGACAAT GCGCTACGCG CACCGGCTCC
     5521 GCCCGTGGAC AACCGCAAGC GGTTGCCCAC CGTCGAGCGC CTTTGCCCAC AACCCGGCGG
     5581 CCGGCCGCAA CAGATCGTTT TATAAATTTT TTTTTTTGAA AAAGAAAAAG CCCGAAAGGC
     5641 GGCAACCTCT CGGGCTTCTG GATTTCCGAT CCccggaatt agactctggc tgcctgtatc
     5701 gagtggtgat tttgtgccga gctgccggtc ggggagctgt tggctggctg g
//