LOCUS       pJAB2514_proG1090::TetR 5544 bp ds-DNA     circular     30-JUN-2022
DEFINITION  .
FEATURES             Location/Qualifiers
     misc_feature    1..25
                     /label="T-Border (left)"
                     /ApEinfo_revcolor=#808000
                     /ApEinfo_fwdcolor=#808000
     terminator      complement(76..359)
                     /label="NOS\terminator"
                     /ApEinfo_revcolor=#d59687
                     /ApEinfo_fwdcolor=#d59687
     misc_marker     complement(367..918)
                     /label="BAR"
                     /ApEinfo_revcolor=#84b0dc
                     /ApEinfo_fwdcolor=#84b0dc
     misc_feature    complement(640..640)
                     /label="G->T mutation to remove Bbs1 site"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     promoter        complement(935..1227)
                     /label="pNOS"
                     /ApEinfo_revcolor=#84b0dc
                     /ApEinfo_fwdcolor=#84b0dc
     misc_feature    1265..1270
                     /label="Bbs1"
                     /ApEinfo_revcolor=#ff0000
                     /ApEinfo_fwdcolor=#ff0000
     misc_feature    1273..1276
                     /label="Bsa1 seam"
                     /ApEinfo_revcolor=#f8d3a9
                     /ApEinfo_fwdcolor=#f8d3a9
     misc_feature    1279..1282
                     /label="Bsa1 seam"
                     /ApEinfo_revcolor=#f8d3a9
                     /ApEinfo_fwdcolor=#f8d3a9
     misc_feature    1283..1525
                     /label="G10-90 promoter"
                     /ApEinfo_revcolor=#84b0dc
                     /ApEinfo_fwdcolor=#84b0dc
     misc_feature    1526..1529
                     /label="Bsa1 seam"
                     /ApEinfo_revcolor=#f8d3a9
                     /ApEinfo_fwdcolor=#f8d3a9
     misc_feature    1530..2150
                     /label="TetR"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     misc_feature    2151..2171
                     /label="SV40 NLS"
                     /ApEinfo_revcolor=#84b0dc
                     /ApEinfo_fwdcolor=#84b0dc
     misc_feature    2172..2174
                     /label="Activator stop codon"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     misc_feature    2175..2178
                     /label="Bsa1 seam"
                     /ApEinfo_revcolor=#f8d3a9
                     /ApEinfo_fwdcolor=#f8d3a9
     terminator      2182..2406
                     /label="19STer"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     misc_feature    2407..2410
                     /label="Bsa1 seam"
                     /ApEinfo_revcolor=#f8d3a9
                     /ApEinfo_fwdcolor=#f8d3a9
     misc_feature    2413..2416
                     /label="Bsa1 seam"
                     /ApEinfo_revcolor=#f8d3a9
                     /ApEinfo_fwdcolor=#f8d3a9
     misc_feature    complement(2419..2424)
                     /label="Bbs1"
                     /ApEinfo_revcolor=#ff0000
                     /ApEinfo_fwdcolor=#ff0000
     primer_bind     complement(2483..2502)
                     /label="T3"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     primer_bind     complement(2520..2540)
                     /label="M13-rev"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     misc_feature    2877..2925
                     /label="RB right border"
                     /ApEinfo_revcolor=#b1ff67
                     /ApEinfo_fwdcolor=#b1ff67
     misc_feature    2974..3655
                     /label="colE1"
                     /ApEinfo_revcolor=#7f7f7f
                     /ApEinfo_fwdcolor=#7f7f7f
     misc_marker     complement(3674..4459)
                     /label="Spec-R"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     misc_feature    complement(4460..4671)
                     /label="spec promoter"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     misc_feature    4912..4912
                     /label="C->A mutation"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     misc_feature    4913..5465
                     /label="pSa ori"
                     /ApEinfo_revcolor=#84b0dc
                     /ApEinfo_fwdcolor=#84b0dc
ORIGIN
        1 tggcaggata tattgtggtg taaacaaatt gacgcttaga caacttaata acacattgcg
       61 gacgttttta atgtaatcag cttgcatgcc ggtcgatcta gtaacataga tgacaccgcg
      121 cgcgataatt tatcctagtt tgcgcgctat attttgtttt ctatcgcgta ttaaatgtat
      181 aattgcggga ctctaatcat aaaaacccat ctcataaata acgtcatgca ttacatgtta
      241 attattacat gcttaacgta attcaacaga aattatatga taatcatcgc aagaccggca
      301 acaggattca atcttaagaa actttattgc caaatgtttg aacgatctgc ttgactctag
      361 gggtcatcag atttcggtga cgggcaggac cggacggggc ggcaccggca ggctgaagtc
      421 cagctgccag aaacccacgt catgccagtt cccgtgcttg aagccggccg cccgcagcat
      481 gccgcggggg gcatatccga gcgcctcgtg catgcgcacg ctcgggtcgt tgggcagccc
      541 gatgacagcg accacgctct tgaagccctg tgcctccagg gacttcagca ggtgggtgta
      601 gagcgtggag cccagtcccg tccgctggtg gcggggggaC acgtacacgg tcgactcggc
      661 cgtccagtcg taggcgttgc gtgccttcca gggacccgcg taggcgatgc cggcgacctc
      721 gccgtccacc tcggcgacga gccagggata gcgctcccgc agacggacga ggtcgtccgt
      781 ccactcctgc ggttcctgcg gctcggtacg gaagttgacc gtgcttgtct cgatgtagtg
      841 gttgacgatg gtgcagaccg ccggcatgtc cgcctcggtg gcacggcgga tgtcggccgg
      901 gcgtcgttct gggctcatgg tagatcccct cgatcgagtt gagagtgaat atgagactct
      961 aattggatac cgaggggaat ttatggaacg tcagtggagc atttttgaca agaaatattt
     1021 gctagctgat agtgacctta ggcgactttt gaacgcgcaa taatggtttc tgacgtatgt
     1081 gcttagctca ttaaactcca gaaacccgcg gctcagtggc tccttcaacg ttgcggttct
     1141 gtcagttcca aacgtaaaac ggcttgtccc gcgtcatcgg cgggggtcat aacgtgactc
     1201 ccttaattct ccgctcatga tcgataacat gtcgactatc ggacgagctc aaagtttaac
     1261 gTgtGAAGAC gtgcttctaa tgtagtttaa actgaaggcg ggaaacgaca atctgatcca
     1321 agctcaagct aagcttgcat gcctgcagga tatcgtggat ccaagcttgc cacgtgccgc
     1381 cacgtgccgc cacgtgccgc cacgtgcctc tagaggatcc atctccactg acgtaaggga
     1441 tgacgcacaa tcccactatc cttcgcaaga cccttcctct atataaggaa gttcatttca
     1501 tttggagagg acacgctggg atcCccaccA TGAGCCGGCT GGACAAGAGC AAAGTGATCA
     1561 ACAGCGCCCT GGAACTGCTC AACGAAGTGG GCATCGAGGG CCTGACCACC CGGAAGCTGG
     1621 CTCAGAAACT GGGCGTGGAA CAGCCCACCC TGTACTGGCA CGTGAAGAAC AAGAGAGCCC
     1681 TGCTGGACGC CCTGGCCATC GAGATGCTGG ACCGGCACCA CACCCACTTT TGCCCCCTGG
     1741 AAGGCGAGAG CTGGCAGGAT TTCCTGCGGA ACAACGCCAA GAGCTTCAGA TGCGCTCTGC
     1801 TGTCCCACCG GGATGGCGCC AAAGTGCACC TGGGCACCAG ACCTACCGAG AAGCAGTACG
     1861 AGACACTGGA AAACCAGCTG GCCTTCCTGT GCCAGCAGGG CTTCAGCCTG GAAAATGCCC
     1921 TGTACGCCCT GAGCGCCGTG GGCCACTTTA CACTGGGCTG CGTGCTGGAA GATCAGGAAC
     1981 ACCAGGTCGC CAAAGAGGAA AGAGAGACAC CCACCACCGA CAGCATGCCC CCTCTGCTGA
     2041 GACAGGCCAT TGAGCTGTTC GATCATCAGG GCGCCGAGCC CGCCTTTCTG TTCGGCCTCG
     2101 AGCTGATCAT CTGTGGCCTC GAAAAACAGC TGAAGTGCGA GTCCGGCAGC ccgaagaaga
     2161 agaggaaggt ttagtgtcgg tacgctgaaa tcaccagtct ctctctacaa atctatctct
     2221 ctctattttc tccataaata atgtgtgagt agtttcccga taagggaaat tagggttctt
     2281 atagggtttc gctcatgtgt tgagcatata agaaaccctt agtatgtatt tgtatttgta
     2341 aaatacttct atcaataaaa tttctaattc ctaaaaccaa aatccagtac taaaatccag
     2401 atcgatgctt gatactacGT CTTCgggcta gagcggacta gttctagagc ggccgccacc
     2461 gcggtggagc tccagctttt gttcccttta gtgagggtta attgcgcgct tggcgtaatc
     2521 atggtcatag ctgtttcctg tgtgaaattg ttatccgctc acaattccac acaacatacg
     2581 agccggaagc ataaagtgta aagcctgggg tgcctaatga gtgagctaac tcacattaat
     2641 tgcgttgcgc tcactgcccg ctttccagtc gggaaacctg tcgtgccagc tgcattaatg
     2701 aatcggccaa cgcgcgggga gaggcggttt gcgtattggg cgctcttccg cttcctcgct
     2761 cactgactcg ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc actcaaaggc
     2821 ggtaatacgg ttatccacag aatcagggga taacgcagga aagaacatga aggccttgac
     2881 aggatatatt ggcgggtaaa ctaagtcgct gtatgtgttt gtttgagatc tcatgtgagc
     2941 aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag
     3001 gctccgcccc cctgacgagc atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc
     3061 gacaggacta taaagatacc aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt
     3121 tccgaccctg ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct
     3181 ttctcatagc tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg
     3241 ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc ttatccggta actatcgtct
     3301 tgagtccaac ccggtaagac acgacttatc gccactggca gcagccactg gtaacaggat
     3361 tagcagagcg aggtatgtag gcggtgctac agagttcttg aagtggtggc ctaactacgg
     3421 ctacactaga agaacagtat ttggtatctg Tgctctgctg aagccagtta ccttcggaag
     3481 aagagttggt agctcttgat ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt
     3541 ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc
     3601 tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg tcatgaattc
     3661 ttgttagaca ttatttgccg actaccttgg tgatctcgcc tttcacgtag tgaacaaatt
     3721 cttccaactg atctgcgcgc gaggccaagc gatcttcttg tccaagataa gcctgcctag
     3781 cttcaagtat gacgggctga tactgggccg gcaggcgctc cattgcccag tcggcagcga
     3841 catccttcgg cgcgattttg ccggttactg cgctgtacca aatgcgggac aacgtaagca
     3901 ctacatttcg ctcatcgcca gcccagtcgg gcggcgagtt ccatagcgtt aaggtttcat
     3961 ttagcgcctc aaatagatcc tgttcaggaa ccggatcaaa gagttcctcc gccgctggac
     4021 ctaccaaggc aacgctatgt tctcttgctt ttgtcagcaa gatagccaga tcaatgtcga
     4081 tcgtggctgg ctcgaagata cctgcaagaa tgtcattgcg ctgccattct ccaaattgca
     4141 gttcgcgctt agctggataa cgccacggaa tgatgtcgtc gtgcacaaca atggtgactt
     4201 ctacagcgcg gagaatctcg ctctctccag gggaagccga agtttccaaa aggtcgttga
     4261 tcaaagctcg ccgcgttgtt tcatcaagcc ttacggtcac cgtaaccagc aaatcaatat
     4321 cactgtgtgg cttcaggccg ccatccactg cggagccgta caaatgtacg gccagcaacg
     4381 tcggttcgag atggcgctcg atgacgccaa ctacctctga tagttgagtc gatacttcgg
     4441 cgatcaccgc ttccctcatg atgtttaact cctgaattaa gccgcgccgc gaagcggtgt
     4501 Cggcttgaat gaattgttag gcgtcatcct gtgctcccga gaaccagtac cagtacatcg
     4561 ctgtttcgtt cgagacttga ggtctagttt tatacgtgaa caggtcaatg ccgccgagag
     4621 taaagccaca Ttttgcgtac aaattgcagg caggtacatt gttcgtttgt gCCATGATGA
     4681 TATATTTTTA TCTTGTGCAA TGTAACATCA GAGATTTTGA GACACAACGT GGCTTTGTTG
     4741 AATAAATCGA ACTTTTGCTG AGTTGAAGGA TCAGATCACG CATCTTCCCG ACAACGCAGA
     4801 CCGTTCCGTG GCAAAGCAAA AGTTCAAAAT CACCAACTGG TCCACCTACA ACAAAGCTCT
     4861 CATCAACCGT GGCTCCCTCA CTTTCTGGCT GGATGATGGG GCGATTCAGG CAGATCCCCA
     4921 TCCAACAGCC CGCCGTCGAG CGGGCTTTTT TATCCCCGGA AGCCTGTGGA TAGAGGGTAG
     4981 TTATCCACGT GAAACCGCTA ATGCCCCGCA AAGCCTTGAT TCACGGGGCT TTCCGGCCCG
     5041 CTCCAAAAAC TATCCACGTG AAATCGCTAA TCAGGGTACG TGAAATCGCT AATCGGAGTA
     5101 CGTGAAATCG CTAATAAGGT CACGTGAAAT CGCTAATCAA AAAGGCACGT GAGAACGCTA
     5161 ATAGCCCTTT CAGATCAACA GCTTGCAAAC ACCCCTCGCT CCGGCAAGTA GTTACAGCAA
     5221 GTAGTATGTT CAATTAGCTT TTCAATTATG AATATATATA TCAATTATTG GTCGCCCTTG
     5281 GCTTGTGGAC AATGCGCTAC GCGCACCGGC TCCGCCCGTG GACAACCGCA AGCGGTTGCC
     5341 CACCGTCGAG CGCCTTTGCC CACAACCCGG CGGCCGGCCG CAACAGATCG TTTTATAAAT
     5401 TTTTTTTTTT GAAAAAGAAA AAGCCCGAAA GGCGGCAACC TCTCGGGCTT CTGGATTTCC
     5461 GATCCccgga attagactct ggctgcctgt atcgagtggt gattttgtgc cgagctgccg
     5521 gtcggggagc tgttggctgg ctgg
//