LOCUS       pJAB2519_proG1090::BetI 5508 bp ds-DNA     circular     30-JUN-2022
DEFINITION  .
FEATURES             Location/Qualifiers
     misc_feature    1..25
                     /label="T-Border (left)"
                     /ApEinfo_revcolor=#808000
                     /ApEinfo_fwdcolor=#808000
     terminator      complement(76..359)
                     /label="NOS\terminator"
                     /ApEinfo_revcolor=#d59687
                     /ApEinfo_fwdcolor=#d59687
     misc_marker     complement(367..918)
                     /label="BAR"
                     /ApEinfo_revcolor=#84b0dc
                     /ApEinfo_fwdcolor=#84b0dc
     misc_feature    complement(640..640)
                     /label="G->T mutation to remove Bbs1 site"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     promoter        complement(935..1227)
                     /label="pNOS"
                     /ApEinfo_revcolor=#84b0dc
                     /ApEinfo_fwdcolor=#84b0dc
     misc_feature    1265..1270
                     /label="Bbs1"
                     /ApEinfo_revcolor=#ff0000
                     /ApEinfo_fwdcolor=#ff0000
     misc_feature    1273..1276
                     /label="Bsa1 seam"
                     /ApEinfo_revcolor=#f8d3a9
                     /ApEinfo_fwdcolor=#f8d3a9
     misc_feature    1279..1282
                     /label="Bsa1 seam"
                     /ApEinfo_revcolor=#f8d3a9
                     /ApEinfo_fwdcolor=#f8d3a9
     misc_feature    1283..1525
                     /label="G10-90 promoter"
                     /ApEinfo_revcolor=#84b0dc
                     /ApEinfo_fwdcolor=#84b0dc
     misc_feature    1526..1529
                     /label="Bsa1 seam"
                     /ApEinfo_revcolor=#f8d3a9
                     /ApEinfo_fwdcolor=#f8d3a9
     misc_feature    1530..2114
                     /label="BetI"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     misc_feature    2115..2135
                     /label="SV40 NLS"
                     /ApEinfo_revcolor=#84b0dc
                     /ApEinfo_fwdcolor=#84b0dc
     misc_feature    2136..2138
                     /label="stop"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     misc_feature    2139..2142
                     /label="Bsa1 seam"
                     /ApEinfo_revcolor=#f8d3a9
                     /ApEinfo_fwdcolor=#f8d3a9
     terminator      2146..2370
                     /label="19STer"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     misc_feature    2371..2374
                     /label="Bsa1 seam"
                     /ApEinfo_revcolor=#f8d3a9
                     /ApEinfo_fwdcolor=#f8d3a9
     misc_feature    2377..2380
                     /label="Bsa1 seam"
                     /ApEinfo_revcolor=#f8d3a9
                     /ApEinfo_fwdcolor=#f8d3a9
     misc_feature    complement(2383..2388)
                     /label="Bbs1"
                     /ApEinfo_revcolor=#ff0000
                     /ApEinfo_fwdcolor=#ff0000
     primer_bind     complement(2447..2466)
                     /label="T3"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     primer_bind     complement(2484..2504)
                     /label="M13-rev"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     misc_feature    2841..2889
                     /label="RB right border"
                     /ApEinfo_revcolor=#b1ff67
                     /ApEinfo_fwdcolor=#b1ff67
     misc_feature    2938..3619
                     /label="colE1"
                     /ApEinfo_revcolor=#7f7f7f
                     /ApEinfo_fwdcolor=#7f7f7f
     misc_marker     complement(3638..4423)
                     /label="Spec-R"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     misc_feature    complement(4424..4635)
                     /label="spec promoter"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     misc_feature    4876..4876
                     /label="C->A mutation"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     misc_feature    4877..5429
                     /label="pSa ori"
                     /ApEinfo_revcolor=#84b0dc
                     /ApEinfo_fwdcolor=#84b0dc
ORIGIN
        1 tggcaggata tattgtggtg taaacaaatt gacgcttaga caacttaata acacattgcg
       61 gacgttttta atgtaatcag cttgcatgcc ggtcgatcta gtaacataga tgacaccgcg
      121 cgcgataatt tatcctagtt tgcgcgctat attttgtttt ctatcgcgta ttaaatgtat
      181 aattgcggga ctctaatcat aaaaacccat ctcataaata acgtcatgca ttacatgtta
      241 attattacat gcttaacgta attcaacaga aattatatga taatcatcgc aagaccggca
      301 acaggattca atcttaagaa actttattgc caaatgtttg aacgatctgc ttgactctag
      361 gggtcatcag atttcggtga cgggcaggac cggacggggc ggcaccggca ggctgaagtc
      421 cagctgccag aaacccacgt catgccagtt cccgtgcttg aagccggccg cccgcagcat
      481 gccgcggggg gcatatccga gcgcctcgtg catgcgcacg ctcgggtcgt tgggcagccc
      541 gatgacagcg accacgctct tgaagccctg tgcctccagg gacttcagca ggtgggtgta
      601 gagcgtggag cccagtcccg tccgctggtg gcggggggaC acgtacacgg tcgactcggc
      661 cgtccagtcg taggcgttgc gtgccttcca gggacccgcg taggcgatgc cggcgacctc
      721 gccgtccacc tcggcgacga gccagggata gcgctcccgc agacggacga ggtcgtccgt
      781 ccactcctgc ggttcctgcg gctcggtacg gaagttgacc gtgcttgtct cgatgtagtg
      841 gttgacgatg gtgcagaccg ccggcatgtc cgcctcggtg gcacggcgga tgtcggccgg
      901 gcgtcgttct gggctcatgg tagatcccct cgatcgagtt gagagtgaat atgagactct
      961 aattggatac cgaggggaat ttatggaacg tcagtggagc atttttgaca agaaatattt
     1021 gctagctgat agtgacctta ggcgactttt gaacgcgcaa taatggtttc tgacgtatgt
     1081 gcttagctca ttaaactcca gaaacccgcg gctcagtggc tccttcaacg ttgcggttct
     1141 gtcagttcca aacgtaaaac ggcttgtccc gcgtcatcgg cgggggtcat aacgtgactc
     1201 ccttaattct ccgctcatga tcgataacat gtcgactatc ggacgagctc aaagtttaac
     1261 gTgtGAAGAC gtgcttctaa tgtagtttaa actgaaggcg ggaaacgaca atctgatcca
     1321 agctcaagct aagcttgcat gcctgcagga tatcgtggat ccaagcttgc cacgtgccgc
     1381 cacgtgccgc cacgtgccgc cacgtgcctc tagaggatcc atctccactg acgtaaggga
     1441 tgacgcacaa tcccactatc cttcgcaaga cccttcctct atataaggaa gttcatttca
     1501 tttggagagg acacgctggg atcCccaccA TGCCGAAACT GGGTATGCAG AGCATTCGTC
     1561 GTCGTCAGCT GATTGATGCA ACCCTGGAAG CAATTAATGA AGTTGGTATG CATGATGCAA
     1621 CCATTGCACA GATTGCACGT CGTGCCGGTG TTAGCACCGG TATTATTAGC CATTATTTCC
     1681 GCGATAAAAA CGGTCTGCTG GAAGCAACCA TGCGTGATAT TACCAGCCAG CTGCGTGATG
     1741 CAGTTCTGAA TCGTCTGCAT GCACTGCCGC AGGGTAGCGC AGAACAGCGT CTGCAGGCAA
     1801 TTGTTGGTGG TAATTTTGAT GAAACCCAGG TTAGCAGCGC AGCAATGAAA GCATGGCTGG
     1861 CATTTTGGGC AAGCAGCATG CATCAGCCGA TGCTGTATCG TCTGCAGCAG GTTAGCAGTC
     1921 GTCGTCTGCT GAGCAATCTG GTTAGCGAAT TTCGTCGTGA ACTGCCTCGT GAACAGGCAC
     1981 AAGAGGCAGG TTATGGTCTG GCAGCACTGA TTGATGGTCT GTGGCTGCGT GCAGCACTGA
     2041 GCGGTAAACC GCTGGATAAA ACCCGTGCAA ATAGCCTGAC CCGTCATTTT ATCACCCAGC
     2101 ATCTGCCGAC CGATccgaag aagaagagga aggttTAGtg tcggtacgct gaaatcacca
     2161 gtctctctct acaaatctat ctctctctat tttctccata aataatgtgt gagtagtttc
     2221 ccgataaggg aaattagggt tcttataggg tttcgctcat gtgttgagca tataagaaac
     2281 ccttagtatg tatttgtatt tgtaaaatac ttctatcaat aaaatttcta attcctaaaa
     2341 ccaaaatcca gtactaaaat ccagatcgat gcttgatact acGTCTTCgg gctagagcgg
     2401 actagttcta gagcggccgc caccgcggtg gagctccagc ttttgttccc tttagtgagg
     2461 gttaattgcg cgcttggcgt aatcatggtc atagctgttt cctgtgtgaa attgttatcc
     2521 gctcacaatt ccacacaaca tacgagccgg aagcataaag tgtaaagcct ggggtgccta
     2581 atgagtgagc taactcacat taattgcgtt gcgctcactg cccgctttcc agtcgggaaa
     2641 cctgtcgtgc cagctgcatt aatgaatcgg ccaacgcgcg gggagaggcg gtttgcgtat
     2701 tgggcgctct tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg
     2761 agcggtatca gctcactcaa aggcggtaat acggttatcc acagaatcag gggataacgc
     2821 aggaaagaac atgaaggcct tgacaggata tattggcggg taaactaagt cgctgtatgt
     2881 gtttgtttga gatctcatgt gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc
     2941 cgcgttgctg gcgtttttcc ataggctccg cccccctgac gagcatcaca aaaatcgacg
     3001 ctcaagtcag aggtggcgaa acccgacagg actataaaga taccaggcgt ttccccctgg
     3061 aagctccctc gtgcgctctc ctgttccgac cctgccgctt accggatacc tgtccgcctt
     3121 tctcccttcg ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc tcagttcggt
     3181 gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg
     3241 cgccttatcc ggtaactatc gtcttgagtc caacccggta agacacgact tatcgccact
     3301 ggcagcagcc actggtaaca ggattagcag agcgaggtat gtaggcggtg ctacagagtt
     3361 cttgaagtgg tggcctaact acggctacac tagaagaaca gtatttggta tctgTgctct
     3421 gctgaagcca gttaccttcg gaagaagagt tggtagctct tgatccggca aacaaaccac
     3481 cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc
     3541 tcaagaagat cctttgatct tttctacggg gtctgacgct cagtggaacg aaaactcacg
     3601 ttaagggatt ttggtcatga attcttgtta gacattattt gccgactacc ttggtgatct
     3661 cgcctttcac gtagtgaaca aattcttcca actgatctgc gcgcgaggcc aagcgatctt
     3721 cttgtccaag ataagcctgc ctagcttcaa gtatgacggg ctgatactgg gccggcaggc
     3781 gctccattgc ccagtcggca gcgacatcct tcggcgcgat tttgccggtt actgcgctgt
     3841 accaaatgcg ggacaacgta agcactacat ttcgctcatc gccagcccag tcgggcggcg
     3901 agttccatag cgttaaggtt tcatttagcg cctcaaatag atcctgttca ggaaccggat
     3961 caaagagttc ctccgccgct ggacctacca aggcaacgct atgttctctt gcttttgtca
     4021 gcaagatagc cagatcaatg tcgatcgtgg ctggctcgaa gatacctgca agaatgtcat
     4081 tgcgctgcca ttctccaaat tgcagttcgc gcttagctgg ataacgccac ggaatgatgt
     4141 cgtcgtgcac aacaatggtg acttctacag cgcggagaat ctcgctctct ccaggggaag
     4201 ccgaagtttc caaaaggtcg ttgatcaaag ctcgccgcgt tgtttcatca agccttacgg
     4261 tcaccgtaac cagcaaatca atatcactgt gtggcttcag gccgccatcc actgcggagc
     4321 cgtacaaatg tacggccagc aacgtcggtt cgagatggcg ctcgatgacg ccaactacct
     4381 ctgatagttg agtcgatact tcggcgatca ccgcttccct catgatgttt aactcctgaa
     4441 ttaagccgcg ccgcgaagcg gtgtCggctt gaatgaattg ttaggcgtca tcctgtgctc
     4501 ccgagaacca gtaccagtac atcgctgttt cgttcgagac ttgaggtcta gttttatacg
     4561 tgaacaggtc aatgccgccg agagtaaagc cacaTtttgc gtacaaattg caggcaggta
     4621 cattgttcgt ttgtgCCATG ATGATATATT TTTATCTTGT GCAATGTAAC ATCAGAGATT
     4681 TTGAGACACA ACGTGGCTTT GTTGAATAAA TCGAACTTTT GCTGAGTTGA AGGATCAGAT
     4741 CACGCATCTT CCCGACAACG CAGACCGTTC CGTGGCAAAG CAAAAGTTCA AAATCACCAA
     4801 CTGGTCCACC TACAACAAAG CTCTCATCAA CCGTGGCTCC CTCACTTTCT GGCTGGATGA
     4861 TGGGGCGATT CAGGCAGATC CCCATCCAAC AGCCCGCCGT CGAGCGGGCT TTTTTATCCC
     4921 CGGAAGCCTG TGGATAGAGG GTAGTTATCC ACGTGAAACC GCTAATGCCC CGCAAAGCCT
     4981 TGATTCACGG GGCTTTCCGG CCCGCTCCAA AAACTATCCA CGTGAAATCG CTAATCAGGG
     5041 TACGTGAAAT CGCTAATCGG AGTACGTGAA ATCGCTAATA AGGTCACGTG AAATCGCTAA
     5101 TCAAAAAGGC ACGTGAGAAC GCTAATAGCC CTTTCAGATC AACAGCTTGC AAACACCCCT
     5161 CGCTCCGGCA AGTAGTTACA GCAAGTAGTA TGTTCAATTA GCTTTTCAAT TATGAATATA
     5221 TATATCAATT ATTGGTCGCC CTTGGCTTGT GGACAATGCG CTACGCGCAC CGGCTCCGCC
     5281 CGTGGACAAC CGCAAGCGGT TGCCCACCGT CGAGCGCCTT TGCCCACAAC CCGGCGGCCG
     5341 GCCGCAACAG ATCGTTTTAT AAATTTTTTT TTTTGAAAAA GAAAAAGCCC GAAAGGCGGC
     5401 AACCTCTCGG GCTTCTGGAT TTCCGATCCc cggaattaga ctctggctgc ctgtatcgag
     5461 tggtgatttt gtgccgagct gccggtcggg gagctgttgg ctggctgg
//