LOCUS       pJAB2618_proG1090::LacI 6246 bp ds-DNA     circular     30-JUN-2022
DEFINITION  .
FEATURES             Location/Qualifiers
     misc_feature    1..25
                     /label="T-Border (left)"
                     /ApEinfo_revcolor=#808000
                     /ApEinfo_fwdcolor=#808000
     terminator      complement(76..359)
                     /label="NOS\terminator"
                     /ApEinfo_revcolor=#d59687
                     /ApEinfo_fwdcolor=#d59687
     misc_marker     complement(367..918)
                     /label="BAR"
                     /ApEinfo_revcolor=#84b0dc
                     /ApEinfo_fwdcolor=#84b0dc
     misc_feature    complement(640..640)
                     /label="G->T mutation to remove Bbs1 site"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     promoter        complement(935..1227)
                     /label="pNOS"
                     /ApEinfo_revcolor=#84b0dc
                     /ApEinfo_fwdcolor=#84b0dc
     misc_feature    1265..1270
                     /label="Bbs1"
                     /ApEinfo_revcolor=#ff0000
                     /ApEinfo_fwdcolor=#ff0000
     misc_feature    1273..1276
                     /label="Bsa1 seam"
                     /ApEinfo_revcolor=#f8d3a9
                     /ApEinfo_fwdcolor=#f8d3a9
     misc_feature    1279..1282
                     /label="Bsa1 seam"
                     /ApEinfo_revcolor=#f8d3a9
                     /ApEinfo_fwdcolor=#f8d3a9
     misc_feature    1283..1525
                     /label="G10-90 promoter"
                     /ApEinfo_revcolor=#84b0dc
                     /ApEinfo_fwdcolor=#84b0dc
     misc_feature    1526..1529
                     /label="Bsa1 seam"
                     /ApEinfo_revcolor=#f8d3a9
                     /ApEinfo_fwdcolor=#f8d3a9
     misc_feature    1530..2609
                     /label="LacI"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     misc_feature    2610..2618
                     /label="Gly-Gly-Gly linker"
                     /ApEinfo_revcolor=#5f76b8
                     /ApEinfo_fwdcolor=#5f76b8
     misc_feature    2619..2852
                     /label="VP16 Hasseloff"
                     /ApEinfo_revcolor=#6e42fa
                     /ApEinfo_fwdcolor=#6e42fa
     misc_feature    2853..2873
                     /label="SV40 NLS"
                     /ApEinfo_revcolor=#84b0dc
                     /ApEinfo_fwdcolor=#84b0dc
     misc_feature    2874..2876
                     /label="stop"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     misc_feature    2877..2880
                     /label="Bsa1 seam"
                     /ApEinfo_revcolor=#f8d3a9
                     /ApEinfo_fwdcolor=#f8d3a9
     terminator      2884..3108
                     /label="19STer"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     misc_feature    3109..3112
                     /label="Bsa1 seam"
                     /ApEinfo_revcolor=#f8d3a9
                     /ApEinfo_fwdcolor=#f8d3a9
     misc_feature    3115..3118
                     /label="Bsa1 seam"
                     /ApEinfo_revcolor=#f8d3a9
                     /ApEinfo_fwdcolor=#f8d3a9
     misc_feature    complement(3121..3126)
                     /label="Bbs1"
                     /ApEinfo_revcolor=#ff0000
                     /ApEinfo_fwdcolor=#ff0000
     primer_bind     complement(3185..3204)
                     /label="T3"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     primer_bind     complement(3222..3242)
                     /label="M13-rev"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     misc_feature    3579..3627
                     /label="RB right border"
                     /ApEinfo_revcolor=#b1ff67
                     /ApEinfo_fwdcolor=#b1ff67
     misc_feature    3676..4357
                     /label="colE1"
                     /ApEinfo_revcolor=#7f7f7f
                     /ApEinfo_fwdcolor=#7f7f7f
     misc_marker     complement(4376..5161)
                     /label="Spec-R"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     misc_feature    complement(5162..5373)
                     /label="spec promoter"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     misc_feature    5614..5614
                     /label="C->A mutation"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     misc_feature    5615..6167
                     /label="pSa ori"
                     /ApEinfo_revcolor=#84b0dc
                     /ApEinfo_fwdcolor=#84b0dc
ORIGIN
        1 tggcaggata tattgtggtg taaacaaatt gacgcttaga caacttaata acacattgcg
       61 gacgttttta atgtaatcag cttgcatgcc ggtcgatcta gtaacataga tgacaccgcg
      121 cgcgataatt tatcctagtt tgcgcgctat attttgtttt ctatcgcgta ttaaatgtat
      181 aattgcggga ctctaatcat aaaaacccat ctcataaata acgtcatgca ttacatgtta
      241 attattacat gcttaacgta attcaacaga aattatatga taatcatcgc aagaccggca
      301 acaggattca atcttaagaa actttattgc caaatgtttg aacgatctgc ttgactctag
      361 gggtcatcag atttcggtga cgggcaggac cggacggggc ggcaccggca ggctgaagtc
      421 cagctgccag aaacccacgt catgccagtt cccgtgcttg aagccggccg cccgcagcat
      481 gccgcggggg gcatatccga gcgcctcgtg catgcgcacg ctcgggtcgt tgggcagccc
      541 gatgacagcg accacgctct tgaagccctg tgcctccagg gacttcagca ggtgggtgta
      601 gagcgtggag cccagtcccg tccgctggtg gcggggggaC acgtacacgg tcgactcggc
      661 cgtccagtcg taggcgttgc gtgccttcca gggacccgcg taggcgatgc cggcgacctc
      721 gccgtccacc tcggcgacga gccagggata gcgctcccgc agacggacga ggtcgtccgt
      781 ccactcctgc ggttcctgcg gctcggtacg gaagttgacc gtgcttgtct cgatgtagtg
      841 gttgacgatg gtgcagaccg ccggcatgtc cgcctcggtg gcacggcgga tgtcggccgg
      901 gcgtcgttct gggctcatgg tagatcccct cgatcgagtt gagagtgaat atgagactct
      961 aattggatac cgaggggaat ttatggaacg tcagtggagc atttttgaca agaaatattt
     1021 gctagctgat agtgacctta ggcgactttt gaacgcgcaa taatggtttc tgacgtatgt
     1081 gcttagctca ttaaactcca gaaacccgcg gctcagtggc tccttcaacg ttgcggttct
     1141 gtcagttcca aacgtaaaac ggcttgtccc gcgtcatcgg cgggggtcat aacgtgactc
     1201 ccttaattct ccgctcatga tcgataacat gtcgactatc ggacgagctc aaagtttaac
     1261 gTgtGAAGAC gtgcttctaa tgtagtttaa actgaaggcg ggaaacgaca atctgatcca
     1321 agctcaagct aagcttgcat gcctgcagga tatcgtggat ccaagcttgc cacgtgccgc
     1381 cacgtgccgc cacgtgccgc cacgtgcctc tagaggatcc atctccactg acgtaaggga
     1441 tgacgcacaa tcccactatc cttcgcaaga cccttcctct atataaggaa gttcatttca
     1501 tttggagagg acacgctggg atcCccaccA TGAAACCAGT AACGTTATAC GATGTCGCAG
     1561 AGTATGCCGG TGTCTCTTAT CAGACCGTTT CCCGCGTGGT GAACCAGGCC AGCCACGTTT
     1621 CTGCGAAAAC GCGGGAAAAA GTGGAAGCGG CGATGGCGGA GCTGAATTAC ATTCCCAACC
     1681 GCGTGGCACA ACAACTGGCG GGCAAACAGT CGTTGCTGAT TGGCGTTGCC ACCTCCAGTC
     1741 TGGCCCTGCA CGCGCCGTCG CAAATTGTCG CGGCGATTAA ATCTCGCGCC GATCAACTGG
     1801 GTGCCAGCGT GGTGGTGTCG ATGGTAGAAC GAAGCGGCGT CGAAGCCTGT AAAGCGGCGG
     1861 TGCACAATCT TCTCGCGCAA CGCGTCAGTG GGCTGATCAT TAACTATCCG CTGGATGACC
     1921 AGGATGCCAT TGCTGTGGAA GCTGCCTGCA CTAATGTTCC GGCGTTATTT CTTGATGTCT
     1981 CTGACCAGAC ACCCATCAAC AGTATTATTT TCTCCCATGA GGACGGTACG CGACTGGGCG
     2041 TGGAGCATCT GGTCGCATTG GGTCACCAGC AAATCGCGCT GTTAGCGGGC CCATTAAGTT
     2101 CTGTCTCGGC GCGTCTGCGT CTGGCTGGCT GGCATAAATA TCTCACTCGC AATCAAATTC
     2161 AGCCGATAGC GGAACGGGAA GGCGACTGGA GTGCCATGTC CGGTTTTCAA CAAACCATGC
     2221 AAATGCTGAA TGAGGGCATC GTTCCCACTG CGATGCTGGT TGCCAACGAT CAGATGGCGC
     2281 TGGGCGCAAT GCGCGCCATT ACCGAGTCCG GGCTGCGCGT TGGTGCGGAT ATCTCGGTAG
     2341 TGGGATACGA CGATACCGAA GATAGCTCAT GTTATATCCC GCCGTTAACC ACCATCAAAC
     2401 AGGATTTTCG CCTGCTGGGG CAAACCAGCG TGGACCGCTT GCTGCAACTC TCTCAGGGCC
     2461 AGGCGGTGAA GGGCAATCAG CTGTTGCCAG TCTCACTGGT GAAAAGAAAA ACCACCCTGG
     2521 CGCCCAATAC GCAAACCGCC TCTCCCCGCG CGTTGGCCGA TTCATTAATG CAGCTGGCAC
     2581 GACAGGTTTC CCGACTGGAA AGCGGGCAGG GAGGAGGTGC CCCCCCGACC GACGTCAGCC
     2641 TGGGGGACGA GCTCCACTTA GACGGCGAGG ACGTGGCGAT GGCGCATGCC GACGCGCTAG
     2701 ACGATTTCGA TCTGGACATG TTGGGGGACG GGGATTCCCC GGGGCCGGGA TTTACCCCCC
     2761 ACGACTCCGC CCCCTACGGC GCTCTGGATA CGGCCGACTT CGAGTTTGAG CAGATGTTTA
     2821 CCGATGCCCT TGGAATTGAC GAGTACGGTG GGccgaagaa gaagaggaag gttTAGtgtc
     2881 ggtacgctga aatcaccagt ctctctctac aaatctatct ctctctattt tctccataaa
     2941 taatgtgtga gtagtttccc gataagggaa attagggttc ttatagggtt tcgctcatgt
     3001 gttgagcata taagaaaccc ttagtatgta tttgtatttg taaaatactt ctatcaataa
     3061 aatttctaat tcctaaaacc aaaatccagt actaaaatcc agatcgatgc ttgatactac
     3121 GTCTTCgggc tagagcggac tagttctaga gcggccgcca ccgcggtgga gctccagctt
     3181 ttgttccctt tagtgagggt taattgcgcg cttggcgtaa tcatggtcat agctgtttcc
     3241 tgtgtgaaat tgttatccgc tcacaattcc acacaacata cgagccggaa gcataaagtg
     3301 taaagcctgg ggtgcctaat gagtgagcta actcacatta attgcgttgc gctcactgcc
     3361 cgctttccag tcgggaaacc tgtcgtgcca gctgcattaa tgaatcggcc aacgcgcggg
     3421 gagaggcggt ttgcgtattg ggcgctcttc cgcttcctcg ctcactgact cgctgcgctc
     3481 ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag gcggtaatac ggttatccac
     3541 agaatcaggg gataacgcag gaaagaacat gaaggccttg acaggatata ttggcgggta
     3601 aactaagtcg ctgtatgtgt ttgtttgaga tctcatgtga gcaaaaggcc agcaaaaggc
     3661 caggaaccgt aaaaaggccg cgttgctggc gtttttccat aggctccgcc cccctgacga
     3721 gcatcacaaa aatcgacgct caagtcagag gtggcgaaac ccgacaggac tataaagata
     3781 ccaggcgttt ccccctggaa gctccctcgt gcgctctcct gttccgaccc tgccgcttac
     3841 cggatacctg tccgcctttc tcccttcggg aagcgtggcg ctttctcata gctcacgctg
     3901 taggtatctc agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc
     3961 cgttcagccc gaccgctgcg ccttatccgg taactatcgt cttgagtcca acccggtaag
     4021 acacgactta tcgccactgg cagcagccac tggtaacagg attagcagag cgaggtatgt
     4081 aggcggtgct acagagttct tgaagtggtg gcctaactac ggctacacta gaagaacagt
     4141 atttggtatc tgTgctctgc tgaagccagt taccttcgga agaagagttg gtagctcttg
     4201 atccggcaaa caaaccaccg ctggtagcgg tggttttttt gtttgcaagc agcagattac
     4261 gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt tctacggggt ctgacgctca
     4321 gtggaacgaa aactcacgtt aagggatttt ggtcatgaat tcttgttaga cattatttgc
     4381 cgactacctt ggtgatctcg cctttcacgt agtgaacaaa ttcttccaac tgatctgcgc
     4441 gcgaggccaa gcgatcttct tgtccaagat aagcctgcct agcttcaagt atgacgggct
     4501 gatactgggc cggcaggcgc tccattgccc agtcggcagc gacatccttc ggcgcgattt
     4561 tgccggttac tgcgctgtac caaatgcggg acaacgtaag cactacattt cgctcatcgc
     4621 cagcccagtc gggcggcgag ttccatagcg ttaaggtttc atttagcgcc tcaaatagat
     4681 cctgttcagg aaccggatca aagagttcct ccgccgctgg acctaccaag gcaacgctat
     4741 gttctcttgc ttttgtcagc aagatagcca gatcaatgtc gatcgtggct ggctcgaaga
     4801 tacctgcaag aatgtcattg cgctgccatt ctccaaattg cagttcgcgc ttagctggat
     4861 aacgccacgg aatgatgtcg tcgtgcacaa caatggtgac ttctacagcg cggagaatct
     4921 cgctctctcc aggggaagcc gaagtttcca aaaggtcgtt gatcaaagct cgccgcgttg
     4981 tttcatcaag ccttacggtc accgtaacca gcaaatcaat atcactgtgt ggcttcaggc
     5041 cgccatccac tgcggagccg tacaaatgta cggccagcaa cgtcggttcg agatggcgct
     5101 cgatgacgcc aactacctct gatagttgag tcgatacttc ggcgatcacc gcttccctca
     5161 tgatgtttaa ctcctgaatt aagccgcgcc gcgaagcggt gtCggcttga atgaattgtt
     5221 aggcgtcatc ctgtgctccc gagaaccagt accagtacat cgctgtttcg ttcgagactt
     5281 gaggtctagt tttatacgtg aacaggtcaa tgccgccgag agtaaagcca caTtttgcgt
     5341 acaaattgca ggcaggtaca ttgttcgttt gtgCCATGAT GATATATTTT TATCTTGTGC
     5401 AATGTAACAT CAGAGATTTT GAGACACAAC GTGGCTTTGT TGAATAAATC GAACTTTTGC
     5461 TGAGTTGAAG GATCAGATCA CGCATCTTCC CGACAACGCA GACCGTTCCG TGGCAAAGCA
     5521 AAAGTTCAAA ATCACCAACT GGTCCACCTA CAACAAAGCT CTCATCAACC GTGGCTCCCT
     5581 CACTTTCTGG CTGGATGATG GGGCGATTCA GGCAGATCCC CATCCAACAG CCCGCCGTCG
     5641 AGCGGGCTTT TTTATCCCCG GAAGCCTGTG GATAGAGGGT AGTTATCCAC GTGAAACCGC
     5701 TAATGCCCCG CAAAGCCTTG ATTCACGGGG CTTTCCGGCC CGCTCCAAAA ACTATCCACG
     5761 TGAAATCGCT AATCAGGGTA CGTGAAATCG CTAATCGGAG TACGTGAAAT CGCTAATAAG
     5821 GTCACGTGAA ATCGCTAATC AAAAAGGCAC GTGAGAACGC TAATAGCCCT TTCAGATCAA
     5881 CAGCTTGCAA ACACCCCTCG CTCCGGCAAG TAGTTACAGC AAGTAGTATG TTCAATTAGC
     5941 TTTTCAATTA TGAATATATA TATCAATTAT TGGTCGCCCT TGGCTTGTGG ACAATGCGCT
     6001 ACGCGCACCG GCTCCGCCCG TGGACAACCG CAAGCGGTTG CCCACCGTCG AGCGCCTTTG
     6061 CCCACAACCC GGCGGCCGGC CGCAACAGAT CGTTTTATAA ATTTTTTTTT TTGAAAAAGA
     6121 AAAAGCCCGA AAGGCGGCAA CCTCTCGGGC TTCTGGATTT CCGATCCccg gaattagact
     6181 ctggctgcct gtatcgagtg gtgattttgt gccgagctgc cggtcgggga gctgttggct
     6241 ggctgg
//