LOCUS       pJAB2657_proG1090::LacI 6003 bp ds-DNA     circular     30-JUN-2022
DEFINITION  .
FEATURES             Location/Qualifiers
     misc_feature    1..25
                     /label="T-Border (left)"
                     /ApEinfo_revcolor=#808000
                     /ApEinfo_fwdcolor=#808000
     terminator      complement(76..359)
                     /label="NOS\terminator"
                     /ApEinfo_revcolor=#d59687
                     /ApEinfo_fwdcolor=#d59687
     misc_marker     complement(367..918)
                     /label="BAR"
                     /ApEinfo_revcolor=#84b0dc
                     /ApEinfo_fwdcolor=#84b0dc
     misc_feature    complement(640..640)
                     /label="G->T mutation to remove Bbs1 site"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     promoter        complement(935..1227)
                     /label="pNOS"
                     /ApEinfo_revcolor=#84b0dc
                     /ApEinfo_fwdcolor=#84b0dc
     misc_feature    1265..1270
                     /label="Bbs1"
                     /ApEinfo_revcolor=#ff0000
                     /ApEinfo_fwdcolor=#ff0000
     misc_feature    1273..1276
                     /label="Bsa1 seam"
                     /ApEinfo_revcolor=#f8d3a9
                     /ApEinfo_fwdcolor=#f8d3a9
     misc_feature    1279..1282
                     /label="Bsa1 seam"
                     /ApEinfo_revcolor=#f8d3a9
                     /ApEinfo_fwdcolor=#f8d3a9
     misc_feature    1283..1525
                     /label="G10-90 promoter"
                     /ApEinfo_revcolor=#84b0dc
                     /ApEinfo_fwdcolor=#84b0dc
     misc_feature    1526..1529
                     /label="Bsa1 seam"
                     /ApEinfo_revcolor=#f8d3a9
                     /ApEinfo_fwdcolor=#f8d3a9
     misc_feature    1530..2609
                     /label="LacI"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     misc_feature    2610..2630
                     /label="SV40 NLS"
                     /ApEinfo_revcolor=#84b0dc
                     /ApEinfo_fwdcolor=#84b0dc
     misc_feature    2631..2633
                     /label="Activator stop codon"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     misc_feature    2634..2637
                     /label="Bsa1 seam"
                     /ApEinfo_revcolor=#f8d3a9
                     /ApEinfo_fwdcolor=#f8d3a9
     terminator      2641..2865
                     /label="19STer"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     misc_feature    2866..2869
                     /label="Bsa1 seam"
                     /ApEinfo_revcolor=#f8d3a9
                     /ApEinfo_fwdcolor=#f8d3a9
     misc_feature    2872..2875
                     /label="Bsa1 seam"
                     /ApEinfo_revcolor=#f8d3a9
                     /ApEinfo_fwdcolor=#f8d3a9
     misc_feature    complement(2878..2883)
                     /label="Bbs1"
                     /ApEinfo_revcolor=#ff0000
                     /ApEinfo_fwdcolor=#ff0000
     primer_bind     complement(2942..2961)
                     /label="T3"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     primer_bind     complement(2979..2999)
                     /label="M13-rev"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     misc_feature    3336..3384
                     /label="RB right border"
                     /ApEinfo_revcolor=#b1ff67
                     /ApEinfo_fwdcolor=#b1ff67
     misc_feature    3433..4114
                     /label="colE1"
                     /ApEinfo_revcolor=#7f7f7f
                     /ApEinfo_fwdcolor=#7f7f7f
     misc_marker     complement(4133..4918)
                     /label="Spec-R"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     misc_feature    complement(4919..5130)
                     /label="spec promoter"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     misc_feature    5371..5371
                     /label="C->A mutation"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     misc_feature    5372..5924
                     /label="pSa ori"
                     /ApEinfo_revcolor=#84b0dc
                     /ApEinfo_fwdcolor=#84b0dc
ORIGIN
        1 tggcaggata tattgtggtg taaacaaatt gacgcttaga caacttaata acacattgcg
       61 gacgttttta atgtaatcag cttgcatgcc ggtcgatcta gtaacataga tgacaccgcg
      121 cgcgataatt tatcctagtt tgcgcgctat attttgtttt ctatcgcgta ttaaatgtat
      181 aattgcggga ctctaatcat aaaaacccat ctcataaata acgtcatgca ttacatgtta
      241 attattacat gcttaacgta attcaacaga aattatatga taatcatcgc aagaccggca
      301 acaggattca atcttaagaa actttattgc caaatgtttg aacgatctgc ttgactctag
      361 gggtcatcag atttcggtga cgggcaggac cggacggggc ggcaccggca ggctgaagtc
      421 cagctgccag aaacccacgt catgccagtt cccgtgcttg aagccggccg cccgcagcat
      481 gccgcggggg gcatatccga gcgcctcgtg catgcgcacg ctcgggtcgt tgggcagccc
      541 gatgacagcg accacgctct tgaagccctg tgcctccagg gacttcagca ggtgggtgta
      601 gagcgtggag cccagtcccg tccgctggtg gcggggggaC acgtacacgg tcgactcggc
      661 cgtccagtcg taggcgttgc gtgccttcca gggacccgcg taggcgatgc cggcgacctc
      721 gccgtccacc tcggcgacga gccagggata gcgctcccgc agacggacga ggtcgtccgt
      781 ccactcctgc ggttcctgcg gctcggtacg gaagttgacc gtgcttgtct cgatgtagtg
      841 gttgacgatg gtgcagaccg ccggcatgtc cgcctcggtg gcacggcgga tgtcggccgg
      901 gcgtcgttct gggctcatgg tagatcccct cgatcgagtt gagagtgaat atgagactct
      961 aattggatac cgaggggaat ttatggaacg tcagtggagc atttttgaca agaaatattt
     1021 gctagctgat agtgacctta ggcgactttt gaacgcgcaa taatggtttc tgacgtatgt
     1081 gcttagctca ttaaactcca gaaacccgcg gctcagtggc tccttcaacg ttgcggttct
     1141 gtcagttcca aacgtaaaac ggcttgtccc gcgtcatcgg cgggggtcat aacgtgactc
     1201 ccttaattct ccgctcatga tcgataacat gtcgactatc ggacgagctc aaagtttaac
     1261 gTgtGAAGAC gtgcttctaa tgtagtttaa actgaaggcg ggaaacgaca atctgatcca
     1321 agctcaagct aagcttgcat gcctgcagga tatcgtggat ccaagcttgc cacgtgccgc
     1381 cacgtgccgc cacgtgccgc cacgtgcctc tagaggatcc atctccactg acgtaaggga
     1441 tgacgcacaa tcccactatc cttcgcaaga cccttcctct atataaggaa gttcatttca
     1501 tttggagagg acacgctggg atcCccaccA TGAAACCAGT AACGTTATAC GATGTCGCAG
     1561 AGTATGCCGG TGTCTCTTAT CAGACCGTTT CCCGCGTGGT GAACCAGGCC AGCCACGTTT
     1621 CTGCGAAAAC GCGGGAAAAA GTGGAAGCGG CGATGGCGGA GCTGAATTAC ATTCCCAACC
     1681 GCGTGGCACA ACAACTGGCG GGCAAACAGT CGTTGCTGAT TGGCGTTGCC ACCTCCAGTC
     1741 TGGCCCTGCA CGCGCCGTCG CAAATTGTCG CGGCGATTAA ATCTCGCGCC GATCAACTGG
     1801 GTGCCAGCGT GGTGGTGTCG ATGGTAGAAC GAAGCGGCGT CGAAGCCTGT AAAGCGGCGG
     1861 TGCACAATCT TCTCGCGCAA CGCGTCAGTG GGCTGATCAT TAACTATCCG CTGGATGACC
     1921 AGGATGCCAT TGCTGTGGAA GCTGCCTGCA CTAATGTTCC GGCGTTATTT CTTGATGTCT
     1981 CTGACCAGAC ACCCATCAAC AGTATTATTT TCTCCCATGA GGACGGTACG CGACTGGGCG
     2041 TGGAGCATCT GGTCGCATTG GGTCACCAGC AAATCGCGCT GTTAGCGGGC CCATTAAGTT
     2101 CTGTCTCGGC GCGTCTGCGT CTGGCTGGCT GGCATAAATA TCTCACTCGC AATCAAATTC
     2161 AGCCGATAGC GGAACGGGAA GGCGACTGGA GTGCCATGTC CGGTTTTCAA CAAACCATGC
     2221 AAATGCTGAA TGAGGGCATC GTTCCCACTG CGATGCTGGT TGCCAACGAT CAGATGGCGC
     2281 TGGGCGCAAT GCGCGCCATT ACCGAGTCCG GGCTGCGCGT TGGTGCGGAT ATCTCGGTAG
     2341 TGGGATACGA CGATACCGAA GATAGCTCAT GTTATATCCC GCCGTTAACC ACCATCAAAC
     2401 AGGATTTTCG CCTGCTGGGG CAAACCAGCG TGGACCGCTT GCTGCAACTC TCTCAGGGCC
     2461 AGGCGGTGAA GGGCAATCAG CTGTTGCCAG TCTCACTGGT GAAAAGAAAA ACCACCCTGG
     2521 CGCCCAATAC GCAAACCGCC TCTCCCCGCG CGTTGGCCGA TTCATTAATG CAGCTGGCAC
     2581 GACAGGTTTC CCGACTGGAA AGCGGGCAGc cgaagaagaa gaggaaggtt TAGtgtcggt
     2641 acgctgaaat caccagtctc tctctacaaa tctatctctc tctattttct ccataaataa
     2701 tgtgtgagta gtttcccgat aagggaaatt agggttctta tagggtttcg ctcatgtgtt
     2761 gagcatataa gaaaccctta gtatgtattt gtatttgtaa aatacttcta tcaataaaat
     2821 ttctaattcc taaaaccaaa atccagtact aaaatccaga tcgatgcttg atactacGTC
     2881 TTCgggctag agcggactag ttctagagcg gccgccaccg cggtggagct ccagcttttg
     2941 ttccctttag tgagggttaa ttgcgcgctt ggcgtaatca tggtcatagc tgtttcctgt
     3001 gtgaaattgt tatccgctca caattccaca caacatacga gccggaagca taaagtgtaa
     3061 agcctggggt gcctaatgag tgagctaact cacattaatt gcgttgcgct cactgcccgc
     3121 tttccagtcg ggaaacctgt cgtgccagct gcattaatga atcggccaac gcgcggggag
     3181 aggcggtttg cgtattgggc gctcttccgc ttcctcgctc actgactcgc tgcgctcggt
     3241 cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt tatccacaga
     3301 atcaggggat aacgcaggaa agaacatgaa ggccttgaca ggatatattg gcgggtaaac
     3361 taagtcgctg tatgtgtttg tttgagatct catgtgagca aaaggccagc aaaaggccag
     3421 gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg ctccgccccc ctgacgagca
     3481 tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg acaggactat aaagatacca
     3541 ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg
     3601 atacctgtcc gcctttctcc cttcgggaag cgtggcgctt tctcatagct cacgctgtag
     3661 gtatctcagt tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt
     3721 tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc cggtaagaca
     3781 cgacttatcg ccactggcag cagccactgg taacaggatt agcagagcga ggtatgtagg
     3841 cggtgctaca gagttcttga agtggtggcc taactacggc tacactagaa gaacagtatt
     3901 tggtatctgT gctctgctga agccagttac cttcggaaga agagttggta gctcttgatc
     3961 cggcaaacaa accaccgctg gtagcggtgg tttttttgtt tgcaagcagc agattacgcg
     4021 cagaaaaaaa ggatctcaag aagatccttt gatcttttct acggggtctg acgctcagtg
     4081 gaacgaaaac tcacgttaag ggattttggt catgaattct tgttagacat tatttgccga
     4141 ctaccttggt gatctcgcct ttcacgtagt gaacaaattc ttccaactga tctgcgcgcg
     4201 aggccaagcg atcttcttgt ccaagataag cctgcctagc ttcaagtatg acgggctgat
     4261 actgggccgg caggcgctcc attgcccagt cggcagcgac atccttcggc gcgattttgc
     4321 cggttactgc gctgtaccaa atgcgggaca acgtaagcac tacatttcgc tcatcgccag
     4381 cccagtcggg cggcgagttc catagcgtta aggtttcatt tagcgcctca aatagatcct
     4441 gttcaggaac cggatcaaag agttcctccg ccgctggacc taccaaggca acgctatgtt
     4501 ctcttgcttt tgtcagcaag atagccagat caatgtcgat cgtggctggc tcgaagatac
     4561 ctgcaagaat gtcattgcgc tgccattctc caaattgcag ttcgcgctta gctggataac
     4621 gccacggaat gatgtcgtcg tgcacaacaa tggtgacttc tacagcgcgg agaatctcgc
     4681 tctctccagg ggaagccgaa gtttccaaaa ggtcgttgat caaagctcgc cgcgttgttt
     4741 catcaagcct tacggtcacc gtaaccagca aatcaatatc actgtgtggc ttcaggccgc
     4801 catccactgc ggagccgtac aaatgtacgg ccagcaacgt cggttcgaga tggcgctcga
     4861 tgacgccaac tacctctgat agttgagtcg atacttcggc gatcaccgct tccctcatga
     4921 tgtttaactc ctgaattaag ccgcgccgcg aagcggtgtC ggcttgaatg aattgttagg
     4981 cgtcatcctg tgctcccgag aaccagtacc agtacatcgc tgtttcgttc gagacttgag
     5041 gtctagtttt atacgtgaac aggtcaatgc cgccgagagt aaagccacaT tttgcgtaca
     5101 aattgcaggc aggtacattg ttcgtttgtg CCATGATGAT ATATTTTTAT CTTGTGCAAT
     5161 GTAACATCAG AGATTTTGAG ACACAACGTG GCTTTGTTGA ATAAATCGAA CTTTTGCTGA
     5221 GTTGAAGGAT CAGATCACGC ATCTTCCCGA CAACGCAGAC CGTTCCGTGG CAAAGCAAAA
     5281 GTTCAAAATC ACCAACTGGT CCACCTACAA CAAAGCTCTC ATCAACCGTG GCTCCCTCAC
     5341 TTTCTGGCTG GATGATGGGG CGATTCAGGC AGATCCCCAT CCAACAGCCC GCCGTCGAGC
     5401 GGGCTTTTTT ATCCCCGGAA GCCTGTGGAT AGAGGGTAGT TATCCACGTG AAACCGCTAA
     5461 TGCCCCGCAA AGCCTTGATT CACGGGGCTT TCCGGCCCGC TCCAAAAACT ATCCACGTGA
     5521 AATCGCTAAT CAGGGTACGT GAAATCGCTA ATCGGAGTAC GTGAAATCGC TAATAAGGTC
     5581 ACGTGAAATC GCTAATCAAA AAGGCACGTG AGAACGCTAA TAGCCCTTTC AGATCAACAG
     5641 CTTGCAAACA CCCCTCGCTC CGGCAAGTAG TTACAGCAAG TAGTATGTTC AATTAGCTTT
     5701 TCAATTATGA ATATATATAT CAATTATTGG TCGCCCTTGG CTTGTGGACA ATGCGCTACG
     5761 CGCACCGGCT CCGCCCGTGG ACAACCGCAA GCGGTTGCCC ACCGTCGAGC GCCTTTGCCC
     5821 ACAACCCGGC GGCCGGCCGC AACAGATCGT TTTATAAATT TTTTTTTTTG AAAAAGAAAA
     5881 AGCCCGAAAG GCGGCAACCT CTCGGGCTTC TGGATTTCCG ATCCccggaa ttagactctg
     5941 gctgcctgta tcgagtggtg attttgtgcc gagctgccgg tcggggagct gttggctggc
     6001 tgg
//