LOCUS       pJAB3422_proUBQ10::PIP2 7644 bp ds-DNA     circular     30-JUN-2022
DEFINITION  .
FEATURES             Location/Qualifiers
     misc_feature    1..24
                     /label="LB left border"
                     /ApEinfo_revcolor=#b1ff67
                     /ApEinfo_fwdcolor=#b1ff67
     misc_feature    34..674
                     /label="region used as proUBQ10 promoter (JANB)"
                     /ApEinfo_revcolor=#84b0dc
                     /ApEinfo_fwdcolor=#84b0dc
     misc_feature    369..672
                     /label="UBQ10 5' intron"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     misc_feature    675..678
                     /label="scar"
                     /ApEinfo_revcolor=#f8d3a9
                     /ApEinfo_fwdcolor=#f8d3a9
     CDS             679..1540
                     /label="PIP2A"
                     /ApEinfo_revcolor=#104aff
                     /ApEinfo_fwdcolor=#104aff
     CDS             1546..2256
                     /label="mCherry"
                     /ApEinfo_revcolor=#ff0000
                     /ApEinfo_fwdcolor=#ff0000
     misc_feature    1974..1974
                     /label="G->T mutation to remove Bbs1"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     terminator      2280..2529
                     /label="nos\3'"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     misc_feature    2544..2549
                     /label="Bbs1"
                     /ApEinfo_revcolor=#ff0000
                     /ApEinfo_fwdcolor=#ff0000
     misc_feature    2552..2555
                     /label="Bsa1 seam"
                     /ApEinfo_revcolor=#f8d3a9
                     /ApEinfo_fwdcolor=#f8d3a9
     misc_feature    2556..3409
                     /label="35S Promoter"
                     /ApEinfo_revcolor=#b7e6d7
                     /ApEinfo_fwdcolor=#b7e6d7
     primer          3284..3311
                     /label="sJAB064"
                     /note="sequence: gcaagtggattgatgtgatatctccact"
                     /ApEinfo_revcolor=#b7e6d7
                     /ApEinfo_fwdcolor=#b7e6d7
     misc_feature    3310..3330
                     /label="as-1 (Lam et al, 1989)"
                     /ApEinfo_revcolor=#213e1f
                     /ApEinfo_fwdcolor=#213e1f
     misc_feature    3328..3330
                     /label="CACAAT box P35S"
                     /ApEinfo_revcolor=#00ae00
                     /ApEinfo_fwdcolor=#00ae00
     misc_feature    3331..3333
                     /label="CACAAT box P35S"
                     /ApEinfo_revcolor=#00ae00
                     /ApEinfo_fwdcolor=#00ae00
     misc_feature    3362..3368
                     /label="TATA extended"
                     /ApEinfo_revcolor=#00bb00
                     /ApEinfo_fwdcolor=#00bb00
     misc_feature    3392..3392
                     /label="+1 P35S"
                     /ApEinfo_revcolor=#00c500
                     /ApEinfo_fwdcolor=#00c500
     misc_feature    3410..3413
                     /label="Bsa1 seam"
                     /ApEinfo_revcolor=#f8d3a9
                     /ApEinfo_fwdcolor=#f8d3a9
     misc_feature    3414..3614
                     /label="mGFP6"
                     /ApEinfo_revcolor=#b1ff67
                     /ApEinfo_fwdcolor=#b1ff67
     misc_feature    3615..3803
                     /label="ST-LS1 intron2"
                     /ApEinfo_revcolor=#8493ca
                     /ApEinfo_fwdcolor=#8493ca
     misc_feature    3804..4322
                     /label="mGFP6"
                     /ApEinfo_revcolor=#b1ff67
                     /ApEinfo_fwdcolor=#b1ff67
     misc_feature    3953..3953
                     /label="A->T mutation to remove Bbs1"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     misc_feature    4323..4326
                     /label="Bsa1 seam"
                     /ApEinfo_revcolor=#f8d3a9
                     /ApEinfo_fwdcolor=#f8d3a9
     misc_feature    4327..4577
                     /label="ADH terminator"
                     /ApEinfo_revcolor=#d59687
                     /ApEinfo_fwdcolor=#d59687
     misc_feature    4578..4581
                     /label="Bsa1 seam"
                     /ApEinfo_revcolor=#f8d3a9
                     /ApEinfo_fwdcolor=#f8d3a9
     misc_feature    complement(4584..4589)
                     /label="Bbs1"
                     /ApEinfo_revcolor=#ff0000
                     /ApEinfo_fwdcolor=#ff0000
     primer_bind     complement(4648..4667)
                     /label="T3"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     primer_bind     complement(4685..4705)
                     /label="M13-rev"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     misc_feature    5042..5090
                     /label="RB right border"
                     /ApEinfo_revcolor=#b1ff67
                     /ApEinfo_fwdcolor=#b1ff67
     misc_feature    5139..5820
                     /label="colE1"
                     /ApEinfo_revcolor=#7f7f7f
                     /ApEinfo_fwdcolor=#7f7f7f
     misc_marker     complement(5839..6624)
                     /label="Spec-R"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     misc_feature    complement(6625..6836)
                     /label="spec promoter"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     misc_feature    7076..7076
                     /label="C->A mutation"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     misc_feature    7077..7629
                     /label="pSa ori"
                     /ApEinfo_revcolor=#84b0dc
                     /ApEinfo_fwdcolor=#84b0dc
ORIGIN
        1 tggcaggata tattgtggtg taacgttAAG CTTTGGTTGT CGACGAGTCA GTAATAAACG
       61 GCGTCAAAGT GGTTGCAGCC GGCACACACG AGTCGTGTTT ATCAACTCAA AGCACAAATA
      121 CTTTTCCTCA ACCTAAAAAT AAGGCAATTA GCCAAAAACA ACTTTGCGTG TAAACAACGC
      181 TCAATACACG TGTCATTTTA TTATTAGCTA TTGCTTCACC GCCTTAGCTT TCTCGTGACC
      241 TAGTCGTCCT CGTCTTTTCT TCTTCTTCTT CTATAAAACA ATACCCAAAG AGCTCTTCTT
      301 CTTCACAATT CAGATTTCAA TTTCTCAAAA TCTTAAAAAC TTTCTCTCAA TTCTCTCTAC
      361 CGTGATCAAG GTAAATTTCT GTGTTCCTTA TTCTCTCAAA ATCTTCGATT TTGTTTTCGT
      421 TCGATCCCAA TTTCGTATAT GTTCTTTGGT TTAGATTCTG TTAATCTTAG ATCGTAGACG
      481 ATTTTCTGGG TTTGATCGTT AGATATCATC TTAATTCTCG ATTAGGGTTT CATAGATATC
      541 ATCCGATTTG TTCAAATAAT TTGAGTTTTG TCGAATAATT ACTCTTCGAT TTGTGATTTC
      601 TATCTAGATC TGGTGTTAGT TTCTAGTTTG TGCGATCGAA TTTGTCGATT AATCTGAGTT
      661 TTTCTGATTA ACAGCACCat ggcaaaggat gtggaagccg ttcccggaga aggatttcag
      721 acaagagact atcaagatcc gccaccagct ccgtttattg atggagcgga gctaaagaag
      781 tggtctttct acagagcagt tatcgcAgag ttcgtagcca ctctcctctt cttatacatc
      841 accgttttga cagtcatcgg ttacaagatt cagtccgata ctgatgccgg tggCgtagat
      901 tgcggcggag ttggaatcct cggtatcgct tgggcctttg gtggtatgat cttcatcctc
      961 gtctactgca ccgccggtat ctctggtggt cacattaacc cagcggtgac atttgggcta
     1021 ttcttggcac gtaaagtgtc gttacctagg gccctattgt acataatcgc tcagtgtttg
     1081 ggtgcgattt gtggagttgg ttttgtcaaa gccttccaaa gctcttacta cacccgttac
     1141 ggaggtggag ccaactctct agccgatggc tacagcacag ggaccggtCt agccgcagag
     1201 atcattggta ctttcgttct tgtctacacC gtAttctctg cCactgaccc caaacgtagt
     1261 gcCagagact cccacgttcc ggtgttggcg ccacttccaa tcggatttgc cgtgttcatg
     1321 gtacatttgg ctaccattcc cattaccgga accggaatta acccggcaag gagtttcgga
     1381 gctgccgtaa tctacaacaa gagcaagcca tgggatgacc actggatatt ttgggttgga
     1441 ccattcattg gagctgcgat agctgcattc taccaccaat tcgttctAag agcttcaggt
     1501 tctaagtctc tTggatcatt cagaagtgct gccaacgtcg gatccatggt gagcaagggc
     1561 gaggaggata acatggccat catcaaggag ttcatgcgct tcaaggtgca catggagggc
     1621 tccgtgaacg gccacgagtt cgagatcgag ggcgagggcg agggccgccc ctacgagggc
     1681 acccagaccg ccaagctgaa ggtgaccaag ggtggccccc tgcccttcgc ctgggacatc
     1741 ctgtcccctc agttcatgta cggctccaag gcctacgtga agcaccccgc cgacatcccc
     1801 gactacttga agctgtcctt ccccgagggc ttcaagtggg agcgcgtgat gaacttcgag
     1861 gacggcggcg tggtgaccgt gacccaggac tcctccctgc aggacggcga gttcatctac
     1921 aaggtgaagc tgcgcggcac caacttcccc tccgacggcc ccgtaatgca gaaTAAGACc
     1981 atgggctggg aggcctcctc cgagcggatg taccccgagg acggcgccct gaagggcgag
     2041 atcaagcaga ggctgaagct gaaggacggc ggccactacg acgctgaggt caagaccacc
     2101 tacaaggcca agaagcccgt gcagctgccc ggcgcctaca acgtcaacat caagttggac
     2161 atcacctccc acaacgagga ctacaccatc gtggaacagt acgaacgcgc cgagggccgc
     2221 cactccaccg gcggcatgga Ggagctgtac AAGtaaagcg gccgcccggc tgcaGATCgt
     2281 tcaaacattt ggcaataaag tttcttaaga ttgaatcctg ttgccggtct tgcgatgatt
     2341 atcatataat ttctgttgaa ttacgttaag catgtaataa ttaacatgta atgcatgacg
     2401 ttatttatga gatgggtttt tatgattaga gtcccgcaat tatacattta atacgcgata
     2461 gaaaacaaaa tatagcgcgc aaactaggat aaattatcgc gcgcggtgtc atctatgtta
     2521 ctagatcggg gcgcgccctc gagGAAGACg taatgGGTCC CCAGATTTGC CTTTTCAATT
     2581 TCAGAAAGAA TGCTAACCCA CAGATGGTTA GAGAGGCTTA CGCAGCAGCT CTCATCAAGA
     2641 CGATCTACCC GAGCAATAAT CTCCAGGAAA TCAAATACCT TCCCAAGAAG GTTAAAGATG
     2701 CAGTCAAAAG ATTCAGGACT AACTGCATCA AGAACACAGA GAAAGATATA TTTCTCAAGA
     2761 TCAGAAGTAC TATTCCAGTA TGGACGATTC AAGGCTTGCT TCACAAACCA AGGCAAGTAA
     2821 TAGAGATTGG AGTCTCTAAA AAGGTAGTTC CCACTGAATC AAAGGCCATG GAGTCAAAGA
     2881 TTCAAATAGA GGACCTAACA GAACTCGCCG TAAAGACTGG CGAACAGTTC ATACAGAGTC
     2941 TCTTACGACT CAATGACAAG AAGAAAATCT TCGTCAACAT GGTGGAGCAC GACACACTTG
     3001 TCTACTCCAA AAATATCAAA GATACAGTCT CAGAAGACCA AAGGGCAATT GAGACTTTTC
     3061 AACAAAGGGT AATATCCGGA AACCTCCTCG GATTCCATTG CCCAGCTATC TGTCACTTTA
     3121 TTGTGAAGAT AGTGGAAAAG GAAGGTGGCT CCTACAAATG CCATCATTGC GATAAAGGAA
     3181 AGGCCATCGT TGAAGATGCC TCTGCCGACA GTGGTCCCAA AGATGGACCC CCACCCACGA
     3241 GGAGCATCGT GGAAAAAGAA GACGTTCCAA CCACGTCTTC AAAGCAAGTG GATTGATGTG
     3301 ATATCTCCAC TGACGTAAGG GATGACGCAC AATCCCACTA TCCTTCGCAA GACCCTTCCT
     3361 CTATATAAGG AAGTTCATTT CATTTGGAGA GAACACGGGG GACTCTAGAC ACCATGGTGA
     3421 GTAAAGGAGA AGAACTTTTC ACTGGAGTTG TCCCAATTCT TGTTGAATTA GATGGTGATG
     3481 TTAATGGGCA CAAATTTTCT GTCAGTGGAG AGGGTGAAGG TGATGCAACA TACGGAAAAC
     3541 TTACCCTTAA ATTTATTTGC ACTACTGGAA AACTACCTGT TCCATGGCCA ACCCTGGTCA
     3601 CCACCCTGAC CTACgtaagt ttctgcttct acctttgata tatatataat aattatcatt
     3661 aattagtagt aatataatat ttcaaatatt tttttcaaaa taaaagaatg tagtatatag
     3721 caattgcttt tctgtagttt ataagtgtgt atattttaat ttataacttt tctaatatat
     3781 gaccaaaatt tgttgatgtg cagGGCGTGC AGTGCTTCTC CCGTTACCCT GATCATATGA
     3841 AGCGGCACGA CTTCTTCAAG AGCGCCATGC CTGAGGGATA CGTGCAGGAG AGGACCATCT
     3901 TCTTCAAGGA CGACGGGAAC TACAAGACAC GTGCTGAAGT CAAGTTTGAG GGTGACACCC
     3961 TCGTCAACAG GATCGAGCTT AAGGGAATCG ATTTCAAGGA GGACGGAAAC ATCCTCGGCC
     4021 ACAAGTTGGA ATACAACTAC AACTCCCACA ACGTATACAT CATGGCCGAC AAGCAAAAGA
     4081 ACGGCATCAA AGCCAACTTC AAGACCCGCC ACAACATCGA GGACGGCGGC GTGCAACTCG
     4141 CTGATCATTA TCAACAAAAT ACTCCAATTG GCGATGGCCC TGTCCTTTTA CCAGACAACC
     4201 ATTACCTGTC CACACAATCT GCCCTTTCGA AAGATCCCAA CGAAAAGAGA GATCACATGG
     4261 TCCTTCTTGA GTTTGTAACA GCTGCTGGGA TTACACATGG CATGGATGAA CTATACAAAT
     4321 AAtgtcaagc cattctctcg cagatgatgt tcactttgtg ttttacttcc tttatgcatt
     4381 cacagcaata aaagaaagaa atctccatcg cttttggttt tcttctctgt cttaagttag
     4441 tcgttttcgt gtctaatcta ttacttatca ttgtaataga ctcttcttct attgagattt
     4501 gaatataaac taaaacacat tccattttac tgtgttctca acattcagaa tgcaaacgga
     4561 ctaaccgtag tgactcggct tgaGTCTTCg ggctagagcg gactagttct agagcggccg
     4621 ccaccgcggt ggagctccag cttttgttcc ctttagtgag ggttaattgc gcgcttggcg
     4681 taatcatggt catagctgtt tcctgtgtga aattgttatc cgctcacaat tccacacaac
     4741 atacgagccg gaagcataaa gtgtaaagcc tggggtgcct aatgagtgag ctaactcaca
     4801 ttaattgcgt tgcgctcact gcccgctttc cagtcgggaa acctgtcgtg ccagctgcat
     4861 taatgaatcg gccaacgcgc ggggagaggc ggtttgcgta ttgggcgctc ttccgcttcc
     4921 tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc agctcactca
     4981 aaggcggtaa tacggttatc cacagaatca ggggataacg caggaaagaa catgaaggcc
     5041 ttgacaggat atattggcgg gtaaactaag tcgctgtatg tgtttgtttg agatctcatg
     5101 tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc
     5161 cataggctcc gcccccctga cgagcatcac aaaaatcgac gctcaagtca gaggtggcga
     5221 aacccgacag gactataaag ataccaggcg tttccccctg gaagctccct cgtgcgctct
     5281 cctgttccga ccctgccgct taccggatac ctgtccgcct ttctcccttc gggaagcgtg
     5341 gcgctttctc atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag
     5401 ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct gcgccttatc cggtaactat
     5461 cgtcttgagt ccaacccggt aagacacgac ttatcgccac tggcagcagc cactggtaac
     5521 aggattagca gagcgaggta tgtaggcggt gctacagagt tcttgaagtg gtggcctaac
     5581 tacggctaca ctagaagaac agtatttggt atctgTgctc tgctgaagcc agttaccttc
     5641 ggaagaagag ttggtagctc ttgatccggc aaacaaacca ccgctggtag cggtggtttt
     5701 tttgtttgca agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc
     5761 ttttctacgg ggtctgacgc tcagtggaac gaaaactcac gttaagggat tttggtcatg
     5821 aattcttgtt agacattatt tgccgactac cttggtgatc tcgcctttca cgtagtgaac
     5881 aaattcttcc aactgatctg cgcgcgaggc caagcgatct tcttgtccaa gataagcctg
     5941 cctagcttca agtatgacgg gctgatactg ggccggcagg cgctccattg cccagtcggc
     6001 agcgacatcc ttcggcgcga ttttgccggt tactgcgctg taccaaatgc gggacaacgt
     6061 aagcactaca tttcgctcat cgccagccca gtcgggcggc gagttccata gcgttaaggt
     6121 ttcatttagc gcctcaaata gatcctgttc aggaaccgga tcaaagagtt cctccgccgc
     6181 tggacctacc aaggcaacgc tatgttctct tgcttttgtc agcaagatag ccagatcaat
     6241 gtcgatcgtg gctggctcga agatacctgc aagaatgtca ttgcgctgcc attctccaaa
     6301 ttgcagttcg cgcttagctg gataacgcca cggaatgatg tcgtcgtgca caacaatggt
     6361 gacttctaca gcgcggagaa tctcgctctc tccaggggaa gccgaagttt ccaaaaggtc
     6421 gttgatcaaa gctcgccgcg ttgtttcatc aagccttacg gtcaccgtaa ccagcaaatc
     6481 aatatcactg tgtggcttca ggccgccatc cactgcggag ccgtacaaat gtacggccag
     6541 caacgtcggt tcgagatggc gctcgatgac gccaactacc tctgatagtt gagtcgatac
     6601 ttcggcgatc accgcttccc tcatgatgtt taactcctga attaagccgc gccgcgaagc
     6661 ggtgtCggct tgaatgaatt gttaggcgtc atcctgtgct cccgagaacc agtaccagta
     6721 catcgctgtt tcgttcgaga cttgaggtct agttttatac gtgaacaggt caatgccgcc
     6781 gagagtaaag ccacaTtttg cgtacaaatt gcaggcaggt acattgttcg tttgtgCCAT
     6841 GATGATATAT TTTTATCTTG TGCAATGTAA CATCAGAGAT TTTGAGACAC AACGTGGCTT
     6901 TGTTGAATAA ATCGAACTTT TGCTGAGTTG AAGGATCAGA TCACGCATCT TCCCGACAAC
     6961 GCAGACCGTT CCGTGGCAAA GCAAAAGTTC AAAATCACCA ACTGGTCCAC CTACAACAAA
     7021 GCTCTCATCA ACCGTGGCTC CCTCACTTTC TGGCTGGATG ATGGGGCGAT TCAGGAGATC
     7081 CCCATCCAAC AGCCCGCCGT CGAGCGGGCT TTTTTATCCC CGGAAGCCTG TGGATAGAGG
     7141 GTAGTTATCC ACGTGAAACC GCTAATGCCC CGCAAAGCCT TGATTCACGG GGCTTTCCGG
     7201 CCCGCTCCAA AAACTATCCA CGTGAAATCG CTAATCAGGG TACGTGAAAT CGCTAATCGG
     7261 AGTACGTGAA ATCGCTAATA AGGTCACGTG AAATCGCTAA TCAAAAAGGC ACGTGAGAAC
     7321 GCTAATAGCC CTTTCAGATC AACAGCTTGC AAACACCCCT CGCTCCGGCA AGTAGTTACA
     7381 GCAAGTAGTA TGTTCAATTA GCTTTTCAAT TATGAATATA TATATCAATT ATTGGTCGCC
     7441 CTTGGCTTGT GGACAATGCG CTACGCGCAC CGGCTCCGCC CGTGGACAAC CGCAAGCGGT
     7501 TGCCCACCGT CGAGCGCCTT TGCCCACAAC CCGGCGGCCG GCCGCAACAG ATCGTTTTAT
     7561 AAATTTTTTT TTTTGAAAAA GAAAAAGCCC GAAAGGCGGC AACCTCTCGG GCTTCTGGAT
     7621 TTCCGATCCc cggaattaga ctct
//