LOCUS       pJAB3423_proUBQ10::PIP2 7242 bp ds-DNA     circular     30-JUN-2022
DEFINITION  .
FEATURES             Location/Qualifiers
     misc_feature    1..24
                     /label="LB left border"
                     /ApEinfo_revcolor=#b1ff67
                     /ApEinfo_fwdcolor=#b1ff67
     misc_feature    34..674
                     /label="region used as proUBQ10 promoter (JANB)"
                     /ApEinfo_revcolor=#84b0dc
                     /ApEinfo_fwdcolor=#84b0dc
     misc_feature    369..672
                     /label="UBQ10 5' intron"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     misc_feature    675..678
                     /label="scar"
                     /ApEinfo_revcolor=#f8d3a9
                     /ApEinfo_fwdcolor=#f8d3a9
     CDS             679..1540
                     /label="PIP2A"
                     /ApEinfo_revcolor=#104aff
                     /ApEinfo_fwdcolor=#104aff
     CDS             1546..2256
                     /label="mCherry"
                     /ApEinfo_revcolor=#ff0000
                     /ApEinfo_fwdcolor=#ff0000
     misc_feature    1974..1974
                     /label="G->T mutation to remove Bbs1"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     terminator      2280..2529
                     /label="nos\3'"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     misc_feature    2544..2549
                     /label="Bbs1"
                     /ApEinfo_revcolor=#ff0000
                     /ApEinfo_fwdcolor=#ff0000
     misc_feature    2552..2555
                     /label="Bsa1 seam"
                     /ApEinfo_revcolor=#f8d3a9
                     /ApEinfo_fwdcolor=#f8d3a9
     misc_feature    2556..3196
                     /label="proUBQ10s"
                     /ApEinfo_revcolor=#84b0dc
                     /ApEinfo_fwdcolor=#84b0dc
     misc_feature    3197..3200
                     /label="Bsa1 seam"
                     /ApEinfo_revcolor=#f8d3a9
                     /ApEinfo_fwdcolor=#f8d3a9
     CDS             3201..3397
                     /label="mgfp6"
                     /ApEinfo_revcolor=#b1ff67
                     /ApEinfo_fwdcolor=#b1ff67
     CDS             3398..3401
                     /label="mgfp6"
                     /ApEinfo_revcolor=#b1ff67
                     /ApEinfo_fwdcolor=#b1ff67
     CDS             3402..3920
                     /label="mGFP6"
                     /ApEinfo_revcolor=#b1ff67
                     /ApEinfo_fwdcolor=#b1ff67
     primer          complement(3446..3467)
                     /label="sJAB063"
                     /note="sequence: catggcgctcttgaagaagtcg"
                     /ApEinfo_revcolor=#c6c9d1
                     /ApEinfo_fwdcolor=#c6c9d1
     misc_feature    3551..3551
                     /label="A->T mutation to remove Bbs1"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     misc_feature    3719..3719
                     /label="A->G mutation to remove Bbs1"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     primer          3800..3824
                     /label="sJAB041"
                     /note="sequence: ttacctgtccacacaatctgccctt"
                     /ApEinfo_revcolor=#9eafd2
                     /ApEinfo_fwdcolor=#9eafd2
     misc_feature    3851..3851
                     /label="C->T to remove Bsa1"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     misc_feature    3921..3924
                     /label="Bsa1 seam"
                     /ApEinfo_revcolor=#f8d3a9
                     /ApEinfo_fwdcolor=#f8d3a9
     misc_feature    3925..4175
                     /label="ADH terminator"
                     /ApEinfo_revcolor=#d59687
                     /ApEinfo_fwdcolor=#d59687
     misc_feature    4176..4179
                     /label="Bsa1 seam"
                     /ApEinfo_revcolor=#f8d3a9
                     /ApEinfo_fwdcolor=#f8d3a9
     misc_feature    complement(4182..4187)
                     /label="Bbs1"
                     /ApEinfo_revcolor=#ff0000
                     /ApEinfo_fwdcolor=#ff0000
     primer_bind     complement(4246..4265)
                     /label="T3"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     primer_bind     complement(4283..4303)
                     /label="M13-rev"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     misc_feature    4640..4688
                     /label="RB right border"
                     /ApEinfo_revcolor=#b1ff67
                     /ApEinfo_fwdcolor=#b1ff67
     misc_feature    4737..5418
                     /label="colE1"
                     /ApEinfo_revcolor=#7f7f7f
                     /ApEinfo_fwdcolor=#7f7f7f
     misc_marker     complement(5437..6222)
                     /label="Spec-R"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     misc_feature    complement(6223..6434)
                     /label="spec promoter"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     misc_feature    6674..6674
                     /label="C->A mutation"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     misc_feature    6675..7227
                     /label="pSa ori"
                     /ApEinfo_revcolor=#84b0dc
                     /ApEinfo_fwdcolor=#84b0dc
ORIGIN
        1 tggcaggata tattgtggtg taacgttAAG CTTTGGTTGT CGACGAGTCA GTAATAAACG
       61 GCGTCAAAGT GGTTGCAGCC GGCACACACG AGTCGTGTTT ATCAACTCAA AGCACAAATA
      121 CTTTTCCTCA ACCTAAAAAT AAGGCAATTA GCCAAAAACA ACTTTGCGTG TAAACAACGC
      181 TCAATACACG TGTCATTTTA TTATTAGCTA TTGCTTCACC GCCTTAGCTT TCTCGTGACC
      241 TAGTCGTCCT CGTCTTTTCT TCTTCTTCTT CTATAAAACA ATACCCAAAG AGCTCTTCTT
      301 CTTCACAATT CAGATTTCAA TTTCTCAAAA TCTTAAAAAC TTTCTCTCAA TTCTCTCTAC
      361 CGTGATCAAG GTAAATTTCT GTGTTCCTTA TTCTCTCAAA ATCTTCGATT TTGTTTTCGT
      421 TCGATCCCAA TTTCGTATAT GTTCTTTGGT TTAGATTCTG TTAATCTTAG ATCGTAGACG
      481 ATTTTCTGGG TTTGATCGTT AGATATCATC TTAATTCTCG ATTAGGGTTT CATAGATATC
      541 ATCCGATTTG TTCAAATAAT TTGAGTTTTG TCGAATAATT ACTCTTCGAT TTGTGATTTC
      601 TATCTAGATC TGGTGTTAGT TTCTAGTTTG TGCGATCGAA TTTGTCGATT AATCTGAGTT
      661 TTTCTGATTA ACAGCACCat ggcaaaggat gtggaagccg ttcccggaga aggatttcag
      721 acaagagact atcaagatcc gccaccagct ccgtttattg atggagcgga gctaaagaag
      781 tggtctttct acagagcagt tatcgcAgag ttcgtagcca ctctcctctt cttatacatc
      841 accgttttga cagtcatcgg ttacaagatt cagtccgata ctgatgccgg tggCgtagat
      901 tgcggcggag ttggaatcct cggtatcgct tgggcctttg gtggtatgat cttcatcctc
      961 gtctactgca ccgccggtat ctctggtggt cacattaacc cagcggtgac atttgggcta
     1021 ttcttggcac gtaaagtgtc gttacctagg gccctattgt acataatcgc tcagtgtttg
     1081 ggtgcgattt gtggagttgg ttttgtcaaa gccttccaaa gctcttacta cacccgttac
     1141 ggaggtggag ccaactctct agccgatggc tacagcacag ggaccggtCt agccgcagag
     1201 atcattggta ctttcgttct tgtctacacC gtAttctctg cCactgaccc caaacgtagt
     1261 gcCagagact cccacgttcc ggtgttggcg ccacttccaa tcggatttgc cgtgttcatg
     1321 gtacatttgg ctaccattcc cattaccgga accggaatta acccggcaag gagtttcgga
     1381 gctgccgtaa tctacaacaa gagcaagcca tgggatgacc actggatatt ttgggttgga
     1441 ccattcattg gagctgcgat agctgcattc taccaccaat tcgttctAag agcttcaggt
     1501 tctaagtctc tTggatcatt cagaagtgct gccaacgtcg gatccatggt gagcaagggc
     1561 gaggaggata acatggccat catcaaggag ttcatgcgct tcaaggtgca catggagggc
     1621 tccgtgaacg gccacgagtt cgagatcgag ggcgagggcg agggccgccc ctacgagggc
     1681 acccagaccg ccaagctgaa ggtgaccaag ggtggccccc tgcccttcgc ctgggacatc
     1741 ctgtcccctc agttcatgta cggctccaag gcctacgtga agcaccccgc cgacatcccc
     1801 gactacttga agctgtcctt ccccgagggc ttcaagtggg agcgcgtgat gaacttcgag
     1861 gacggcggcg tggtgaccgt gacccaggac tcctccctgc aggacggcga gttcatctac
     1921 aaggtgaagc tgcgcggcac caacttcccc tccgacggcc ccgtaatgca gaaTAAGACc
     1981 atgggctggg aggcctcctc cgagcggatg taccccgagg acggcgccct gaagggcgag
     2041 atcaagcaga ggctgaagct gaaggacggc ggccactacg acgctgaggt caagaccacc
     2101 tacaaggcca agaagcccgt gcagctgccc ggcgcctaca acgtcaacat caagttggac
     2161 atcacctccc acaacgagga ctacaccatc gtggaacagt acgaacgcgc cgagggccgc
     2221 cactccaccg gcggcatgga Ggagctgtac AAGtaaagcg gccgcccggc tgcaGATCgt
     2281 tcaaacattt ggcaataaag tttcttaaga ttgaatcctg ttgccggtct tgcgatgatt
     2341 atcatataat ttctgttgaa ttacgttaag catgtaataa ttaacatgta atgcatgacg
     2401 ttatttatga gatgggtttt tatgattaga gtcccgcaat tatacattta atacgcgata
     2461 gaaaacaaaa tatagcgcgc aaactaggat aaattatcgc gcgcggtgtc atctatgtta
     2521 ctagatcggg gcgcgccctc gagGAAGACg taatgTGGTT GTCGACGAGT CAGTAATAAA
     2581 CGGCGTCAAA GTGGTTGCAG CCGGCACACA CGAGTCGTGT TTATCAACTC AAAGCACAAA
     2641 TACTTTTCCT CAACCTAAAA ATAAGGCAAT TAGCCAAAAA CAACTTTGCG TGTAAACAAC
     2701 GCTCAATACA CGTGTCATTT TATTATTAGC TATTGCTTCA CCGCCTTAGC TTTCTCGTGA
     2761 CCTAGTCGTC CTCGTCTTTT CTTCTTCTTC TTCTATAAAA CAATACCCAA AGAGCTCTTC
     2821 TTCTTCACAA TTCAGATTTC AATTTCTCAA AATCTTAAAA ACTTTCTCTC AATTCTCTCT
     2881 ACCGTGATCA AGGTAAATTT CTGTGTTCCT TATTCTCTCA AAATCTTCGA TTTTGTTTTC
     2941 GTTCGATCCC AATTTCGTAT ATGTTCTTTG GTTTAGATTC TGTTAATCTT AGATCGAAGA
     3001 CGATTTTCTG GGTTTGATCG TTAGATATCA TCTTAATTCT CGATTAGGGT TTCATAGATA
     3061 TCATCCGATT TGTTCAAATA ATTTGAGTTT TGTCGAATAA TTACTCTTCG ATTTGTGATT
     3121 TCTATCTAGA TCTGGTGTTA GTTTCTAGTT TGTGCGATCG AATTTGTCGA TTAATCTGAG
     3181 TTTTTCTGAT TAACAGCACC ATGGTGAGTA AAGGAGAAGA ACTTTTCACT GGAGTTGTCC
     3241 CAATTCTTGT TGAATTAGAT GGTGATGTTA ATGGGCACAA ATTTTCTGTC AGTGGAGAGG
     3301 GTGAAGGTGA TGCAACATAC GGAAAACTTA CCCTTAAATT TATTTGCACT ACTGGAAAAC
     3361 TACCTGTTCC ATGGCCAACC CTGGTCACCA CCCTGACCTA CGGCGTGCAG TGCTTCTCCC
     3421 GTTACCCTGA TCATATGAAG CGGCACGACT TCTTCAAGAG CGCCATGCCT GAGGGATACG
     3481 TGCAGGAGAG GACCATCTTC TTCAAGGACG ACGGGAACTA CAAGACACGT GCTGAAGTCA
     3541 AGTTTGAGGG TGACACCCTC GTCAACAGGA TCGAGCTTAA GGGAATCGAT TTCAAGGAGG
     3601 ACGGAAACAT CCTCGGCCAC AAGTTGGAAT ACAACTACAA CTCCCACAAC GTATACATCA
     3661 TGGCCGACAA GCAAAAGAAC GGCATCAAAG CCAACTTCAA GACCCGCCAC AACATCGAGG
     3721 ACGGCGGCGT GCAACTCGCT GATCATTATC AACAAAATAC TCCAATTGGC GATGGCCCTG
     3781 TCCTTTTACC AGACAACCAT TACCTGTCCA CACAATCTGC CCTTTCGAAA GATCCCAACG
     3841 AAAAGAGAGA TCACATGGTC CTTCTTGAGT TTGTAACAGC TGCTGGGATT ACACATGGCA
     3901 TGGATGAACT ATACAAATAA TGTCaagcca ttctctcgca gatgatgttc actttgtgtt
     3961 ttacttcctt tatgcattca cagcaataaa agaaagaaat ctccatcgct tttggttttc
     4021 ttctctgtct taagttagtc gttttcgtgt ctaatctatt acttatcatt gtaatagact
     4081 cttcttctat tgagatttga atataaacta aaacacattc cattttactg tgttctcaac
     4141 attcagaatg caaacggact aaccgtagtg actcggcttg aGTCTTCggg ctagagcgga
     4201 ctagttctag agcggccgcc accgcggtgg agctccagct tttgttccct ttagtgaggg
     4261 ttaattgcgc gcttggcgta atcatggtca tagctgtttc ctgtgtgaaa ttgttatccg
     4321 ctcacaattc cacacaacat acgagccgga agcataaagt gtaaagcctg gggtgcctaa
     4381 tgagtgagct aactcacatt aattgcgttg cgctcactgc ccgctttcca gtcgggaaac
     4441 ctgtcgtgcc agctgcatta atgaatcggc caacgcgcgg ggagaggcgg tttgcgtatt
     4501 gggcgctctt ccgcttcctc gctcactgac tcgctgcgct cggtcgttcg gctgcggcga
     4561 gcggtatcag ctcactcaaa ggcggtaata cggttatcca cagaatcagg ggataacgca
     4621 ggaaagaaca tgaaggcctt gacaggatat attggcgggt aaactaagtc gctgtatgtg
     4681 tttgtttgag atctcatgtg agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc
     4741 gcgttgctgg cgtttttcca taggctccgc ccccctgacg agcatcacaa aaatcgacgc
     4801 tcaagtcaga ggtggcgaaa cccgacagga ctataaagat accaggcgtt tccccctgga
     4861 agctccctcg tgcgctctcc tgttccgacc ctgccgctta ccggatacct gtccgccttt
     4921 ctcccttcgg gaagcgtggc gctttctcat agctcacgct gtaggtatct cagttcggtg
     4981 taggtcgttc gctccaagct gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc
     5041 gccttatccg gtaactatcg tcttgagtcc aacccggtaa gacacgactt atcgccactg
     5101 gcagcagcca ctggtaacag gattagcaga gcgaggtatg taggcggtgc tacagagttc
     5161 ttgaagtggt ggcctaacta cggctacact agaagaacag tatttggtat ctgTgctctg
     5221 ctgaagccag ttaccttcgg aagaagagtt ggtagctctt gatccggcaa acaaaccacc
     5281 gctggtagcg gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct
     5341 caagaagatc ctttgatctt ttctacgggg tctgacgctc agtggaacga aaactcacgt
     5401 taagggattt tggtcatgaa ttcttgttag acattatttg ccgactacct tggtgatctc
     5461 gcctttcacg tagtgaacaa attcttccaa ctgatctgcg cgcgaggcca agcgatcttc
     5521 ttgtccaaga taagcctgcc tagcttcaag tatgacgggc tgatactggg ccggcaggcg
     5581 ctccattgcc cagtcggcag cgacatcctt cggcgcgatt ttgccggtta ctgcgctgta
     5641 ccaaatgcgg gacaacgtaa gcactacatt tcgctcatcg ccagcccagt cgggcggcga
     5701 gttccatagc gttaaggttt catttagcgc ctcaaataga tcctgttcag gaaccggatc
     5761 aaagagttcc tccgccgctg gacctaccaa ggcaacgcta tgttctcttg cttttgtcag
     5821 caagatagcc agatcaatgt cgatcgtggc tggctcgaag atacctgcaa gaatgtcatt
     5881 gcgctgccat tctccaaatt gcagttcgcg cttagctgga taacgccacg gaatgatgtc
     5941 gtcgtgcaca acaatggtga cttctacagc gcggagaatc tcgctctctc caggggaagc
     6001 cgaagtttcc aaaaggtcgt tgatcaaagc tcgccgcgtt gtttcatcaa gccttacggt
     6061 caccgtaacc agcaaatcaa tatcactgtg tggcttcagg ccgccatcca ctgcggagcc
     6121 gtacaaatgt acggccagca acgtcggttc gagatggcgc tcgatgacgc caactacctc
     6181 tgatagttga gtcgatactt cggcgatcac cgcttccctc atgatgttta actcctgaat
     6241 taagccgcgc cgcgaagcgg tgtCggcttg aatgaattgt taggcgtcat cctgtgctcc
     6301 cgagaaccag taccagtaca tcgctgtttc gttcgagact tgaggtctag ttttatacgt
     6361 gaacaggtca atgccgccga gagtaaagcc acaTtttgcg tacaaattgc aggcaggtac
     6421 attgttcgtt tgtgCCATGA TGATATATTT TTATCTTGTG CAATGTAACA TCAGAGATTT
     6481 TGAGACACAA CGTGGCTTTG TTGAATAAAT CGAACTTTTG CTGAGTTGAA GGATCAGATC
     6541 ACGCATCTTC CCGACAACGC AGACCGTTCC GTGGCAAAGC AAAAGTTCAA AATCACCAAC
     6601 TGGTCCACCT ACAACAAAGC TCTCATCAAC CGTGGCTCCC TCACTTTCTG GCTGGATGAT
     6661 GGGGCGATTC AGGAGATCCC CATCCAACAG CCCGCCGTCG AGCGGGCTTT TTTATCCCCG
     6721 GAAGCCTGTG GATAGAGGGT AGTTATCCAC GTGAAACCGC TAATGCCCCG CAAAGCCTTG
     6781 ATTCACGGGG CTTTCCGGCC CGCTCCAAAA ACTATCCACG TGAAATCGCT AATCAGGGTA
     6841 CGTGAAATCG CTAATCGGAG TACGTGAAAT CGCTAATAAG GTCACGTGAA ATCGCTAATC
     6901 AAAAAGGCAC GTGAGAACGC TAATAGCCCT TTCAGATCAA CAGCTTGCAA ACACCCCTCG
     6961 CTCCGGCAAG TAGTTACAGC AAGTAGTATG TTCAATTAGC TTTTCAATTA TGAATATATA
     7021 TATCAATTAT TGGTCGCCCT TGGCTTGTGG ACAATGCGCT ACGCGCACCG GCTCCGCCCG
     7081 TGGACAACCG CAAGCGGTTG CCCACCGTCG AGCGCCTTTG CCCACAACCC GGCGGCCGGC
     7141 CGCAACAGAT CGTTTTATAA ATTTTTTTTT TTGAAAAAGA AAAAGCCCGA AAGGCGGCAA
     7201 CCTCTCGGGC TTCTGGATTT CCGATCCccg gaattagact ct
//