LOCUS       pJAB3477_6XPhlFO-3XAmtR 5676 bp ds-DNA     circular     30-JUN-2022
DEFINITION  .
FEATURES             Location/Qualifiers
     misc_feature    1..25
                     /label="T-Border (left)"
                     /ApEinfo_revcolor=#808000
                     /ApEinfo_fwdcolor=#808000
     terminator      complement(76..359)
                     /label="NOS\terminator"
                     /ApEinfo_revcolor=#d59687
                     /ApEinfo_fwdcolor=#d59687
     misc_marker     complement(367..918)
                     /label="BAR"
                     /ApEinfo_revcolor=#84b0dc
                     /ApEinfo_fwdcolor=#84b0dc
     misc_feature    complement(640..640)
                     /label="G->T mutation to remove Bbs1 site"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     promoter        complement(935..1227)
                     /label="pNOS"
                     /ApEinfo_revcolor=#84b0dc
                     /ApEinfo_fwdcolor=#84b0dc
     misc_feature    1265..1270
                     /label="Bbs1"
                     /ApEinfo_revcolor=#ff0000
                     /ApEinfo_fwdcolor=#ff0000
     misc_feature    1273..1276
                     /label="Bsa1 seam"
                     /ApEinfo_revcolor=#f8d3a9
                     /ApEinfo_fwdcolor=#f8d3a9
     misc_feature    1279..1282
                     /label="Bsa1 seam"
                     /ApEinfo_revcolor=#f8d3a9
                     /ApEinfo_fwdcolor=#f8d3a9
     misc_feature    1309..1338
                     /label="PhlFO"
                     /ApEinfo_revcolor=#008080
                     /ApEinfo_fwdcolor=#008080
     misc_feature    1341..1370
                     /label="PhlFO"
                     /ApEinfo_revcolor=#008080
                     /ApEinfo_fwdcolor=#008080
     misc_feature    1373..1402
                     /label="PhlFO"
                     /ApEinfo_revcolor=#008080
                     /ApEinfo_fwdcolor=#008080
     misc_feature    1405..1434
                     /label="PhlFO"
                     /ApEinfo_revcolor=#008080
                     /ApEinfo_fwdcolor=#008080
     misc_feature    1437..1466
                     /label="PhlFA"
                     /ApEinfo_revcolor=#008080
                     /ApEinfo_fwdcolor=#008080
     misc_feature    1469..1498
                     /label="PhlFA"
                     /ApEinfo_revcolor=#008080
                     /ApEinfo_fwdcolor=#008080
     misc_feature    1506..1506
                     /label="C ->A mutation to remove Bsa1"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     misc_feature    1519..1522
                     /label="Bsa1 seam"
                     /ApEinfo_revcolor=#f8d3a9
                     /ApEinfo_fwdcolor=#f8d3a9
     misc_feature    1523..1581
                     /label="pro35S"
                     /ApEinfo_revcolor=#b1ff67
                     /ApEinfo_fwdcolor=#b1ff67
     misc_feature    1549..1569
                     /label="as-1 (Lam et al, 1989)"
                     /ApEinfo_revcolor=#213e1f
                     /ApEinfo_fwdcolor=#213e1f
     misc_feature    1558..1558
                     /label="G deleted"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     misc_feature    1567..1572
                     /label="CACAAT box P35S"
                     /ApEinfo_revcolor=#00ae00
                     /ApEinfo_fwdcolor=#00ae00
     misc_feature    1582..1602
                     /label="AmtRO"
                     /ApEinfo_revcolor=#008080
                     /ApEinfo_fwdcolor=#008080
     misc_feature    1604..1610
                     /label="TATA extended"
                     /ApEinfo_revcolor=#00bb00
                     /ApEinfo_fwdcolor=#00bb00
     misc_feature    1611..1631
                     /label="AmtRO"
                     /ApEinfo_revcolor=#008080
                     /ApEinfo_fwdcolor=#008080
     misc_feature    1634..1654
                     /label="AmtRO"
                     /ApEinfo_revcolor=#008080
                     /ApEinfo_fwdcolor=#008080
     promoter        1655..1657
                     /label="35S Promter"
                     /ApEinfo_revcolor=#b1ff67
                     /ApEinfo_fwdcolor=#b1ff67
     misc_feature    1658..1661
                     /label="Bsa1 seam"
                     /ApEinfo_revcolor=#f8d3a9
                     /ApEinfo_fwdcolor=#f8d3a9
     misc_feature    1662..2282
                     /label="TetR"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     misc_feature    2283..2303
                     /label="SV40 NLS"
                     /ApEinfo_revcolor=#84b0dc
                     /ApEinfo_fwdcolor=#84b0dc
     misc_feature    2304..2306
                     /label="stop"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     misc_feature    2307..2310
                     /label="Bsa1 seam"
                     /ApEinfo_revcolor=#f8d3a9
                     /ApEinfo_fwdcolor=#f8d3a9
     terminator      2314..2538
                     /label="19STer"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     misc_feature    2539..2542
                     /label="Bsa1 seam"
                     /ApEinfo_revcolor=#f8d3a9
                     /ApEinfo_fwdcolor=#f8d3a9
     misc_feature    2545..2548
                     /label="Bsa1 seam"
                     /ApEinfo_revcolor=#f8d3a9
                     /ApEinfo_fwdcolor=#f8d3a9
     misc_feature    complement(2551..2556)
                     /label="Bbs1"
                     /ApEinfo_revcolor=#ff0000
                     /ApEinfo_fwdcolor=#ff0000
     primer_bind     complement(2615..2634)
                     /label="T3"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     primer_bind     complement(2652..2672)
                     /label="M13-rev"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     misc_feature    3009..3057
                     /label="RB right border"
                     /ApEinfo_revcolor=#b1ff67
                     /ApEinfo_fwdcolor=#b1ff67
     misc_feature    3106..3787
                     /label="colE1"
                     /ApEinfo_revcolor=#7f7f7f
                     /ApEinfo_fwdcolor=#7f7f7f
     misc_marker     complement(3806..4591)
                     /label="Spec-R"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     misc_feature    complement(4592..4803)
                     /label="spec promoter"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     misc_feature    5044..5044
                     /label="C->A mutation"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     misc_feature    5045..5597
                     /label="pSa ori"
                     /ApEinfo_revcolor=#84b0dc
                     /ApEinfo_fwdcolor=#84b0dc
ORIGIN
        1 tggcaggata tattgtggtg taaacaaatt gacgcttaga caacttaata acacattgcg
       61 gacgttttta atgtaatcag cttgcatgcc ggtcgatcta gtaacataga tgacaccgcg
      121 cgcgataatt tatcctagtt tgcgcgctat attttgtttt ctatcgcgta ttaaatgtat
      181 aattgcggga ctctaatcat aaaaacccat ctcataaata acgtcatgca ttacatgtta
      241 attattacat gcttaacgta attcaacaga aattatatga taatcatcgc aagaccggca
      301 acaggattca atcttaagaa actttattgc caaatgtttg aacgatctgc ttgactctag
      361 gggtcatcag atttcggtga cgggcaggac cggacggggc ggcaccggca ggctgaagtc
      421 cagctgccag aaacccacgt catgccagtt cccgtgcttg aagccggccg cccgcagcat
      481 gccgcggggg gcatatccga gcgcctcgtg catgcgcacg ctcgggtcgt tgggcagccc
      541 gatgacagcg accacgctct tgaagccctg tgcctccagg gacttcagca ggtgggtgta
      601 gagcgtggag cccagtcccg tccgctggtg gcggggggaC acgtacacgg tcgactcggc
      661 cgtccagtcg taggcgttgc gtgccttcca gggacccgcg taggcgatgc cggcgacctc
      721 gccgtccacc tcggcgacga gccagggata gcgctcccgc agacggacga ggtcgtccgt
      781 ccactcctgc ggttcctgcg gctcggtacg gaagttgacc gtgcttgtct cgatgtagtg
      841 gttgacgatg gtgcagaccg ccggcatgtc cgcctcggtg gcacggcgga tgtcggccgg
      901 gcgtcgttct gggctcatgg tagatcccct cgatcgagtt gagagtgaat atgagactct
      961 aattggatac cgaggggaat ttatggaacg tcagtggagc atttttgaca agaaatattt
     1021 gctagctgat agtgacctta ggcgactttt gaacgcgcaa taatggtttc tgacgtatgt
     1081 gcttagctca ttaaactcca gaaacccgcg gctcagtggc tccttcaacg ttgcggttct
     1141 gtcagttcca aacgtaaaac ggcttgtccc gcgtcatcgg cgggggtcat aacgtgactc
     1201 ccttaattct ccgctcatga tcgataacat gtcgactatc ggacgagctc aaagtttaac
     1261 gTgtGAAGAC gtgcttctaa tgATAGAAAA GTTGGCTGCG GCCGCGAAAT GATACGAAAC
     1321 GTACCGTATC GTTAAGGTGA ATGATACGAA ACGTACCGTA TCGTTAAGGT CAATGATACG
     1381 AAACGTACCG TATCGTTAAG GTCAATGATA CGAAACGTAC CGTATCGTTA AGGTGTATGA
     1441 TACGAAACGT ACCGTATCGT TAAGGTAGAT GATACGAAAC GTACCGTATC GTTAAGGTCG
     1501 GGTCTAAGCT AGCTAGGCTA GCGCAAGTGG ATTGATGTGA TATCTCCACT GACGTAAGGG
     1561 ATGACGCACA ATCCCACTAT CTTCTATCGA TCTATAGATA ATCTATATAA TTCTATCGAT
     1621 CTATAGATAA TCGTTCTATC GATCTATAGA TAATAGACAC CATGAGCCGG CTGGACAAGA
     1681 GCAAAGTGAT CAACAGCGCC CTGGAACTGC TCAACGAAGT GGGCATCGAG GGCCTGACCA
     1741 CCCGGAAGCT GGCTCAGAAA CTGGGCGTGG AACAGCCCAC CCTGTACTGG CACGTGAAGA
     1801 ACAAGAGAGC CCTGCTGGAC GCCCTGGCCA TCGAGATGCT GGACCGGCAC CACACCCACT
     1861 TTTGCCCCCT GGAAGGCGAG AGCTGGCAGG ATTTCCTGCG GAACAACGCC AAGAGCTTCA
     1921 GATGCGCTCT GCTGTCCCAC CGGGATGGCG CCAAAGTGCA CCTGGGCACC AGACCTACCG
     1981 AGAAGCAGTA CGAGACACTG GAAAACCAGC TGGCCTTCCT GTGCCAGCAG GGCTTCAGCC
     2041 TGGAAAATGC CCTGTACGCC CTGAGCGCCG TGGGCCACTT TACACTGGGC TGCGTGCTGG
     2101 AAGATCAGGA ACACCAGGTC GCCAAAGAGG AAAGAGAGAC ACCCACCACC GACAGCATGC
     2161 CCCCTCTGCT GAGACAGGCC ATTGAGCTGT TCGATCATCA GGGCGCCGAG CCCGCCTTTC
     2221 TGTTCGGCCT CGAGCTGATC ATCTGTGGCC TCGAAAAACA GCTGAAGTGC GAGTCCGGCA
     2281 GCccgaagaa gaagaggaag gtttagtgtc ggtacgctga aatcaccagt ctctctctac
     2341 aaatctatct ctctctattt tctccataaa taatgtgtga gtagtttccc gataagggaa
     2401 attagggttc ttatagggtt tcgctcatgt gttgagcata taagaaaccc ttagtatgta
     2461 tttgtatttg taaaatactt ctatcaataa aatttctaat tcctaaaacc aaaatccagt
     2521 actaaaatcc agatcgatgc ttgatactac GTCTTCgggc tagagcggac tagttctaga
     2581 gcggccgcca ccgcggtgga gctccagctt ttgttccctt tagtgagggt taattgcgcg
     2641 cttggcgtaa tcatggtcat agctgtttcc tgtgtgaaat tgttatccgc tcacaattcc
     2701 acacaacata cgagccggaa gcataaagtg taaagcctgg ggtgcctaat gagtgagcta
     2761 actcacatta attgcgttgc gctcactgcc cgctttccag tcgggaaacc tgtcgtgcca
     2821 gctgcattaa tgaatcggcc aacgcgcggg gagaggcggt ttgcgtattg ggcgctcttc
     2881 cgcttcctcg ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag cggtatcagc
     2941 tcactcaaag gcggtaatac ggttatccac agaatcaggg gataacgcag gaaagaacat
     3001 gaaggccttg acaggatata ttggcgggta aactaagtcg ctgtatgtgt ttgtttgaga
     3061 tctcatgtga gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc
     3121 gtttttccat aggctccgcc cccctgacga gcatcacaaa aatcgacgct caagtcagag
     3181 gtggcgaaac ccgacaggac tataaagata ccaggcgttt ccccctggaa gctccctcgt
     3241 gcgctctcct gttccgaccc tgccgcttac cggatacctg tccgcctttc tcccttcggg
     3301 aagcgtggcg ctttctcata gctcacgctg taggtatctc agttcggtgt aggtcgttcg
     3361 ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg ccttatccgg
     3421 taactatcgt cttgagtcca acccggtaag acacgactta tcgccactgg cagcagccac
     3481 tggtaacagg attagcagag cgaggtatgt aggcggtgct acagagttct tgaagtggtg
     3541 gcctaactac ggctacacta gaagaacagt atttggtatc tgTgctctgc tgaagccagt
     3601 taccttcgga agaagagttg gtagctcttg atccggcaaa caaaccaccg ctggtagcgg
     3661 tggttttttt gtttgcaagc agcagattac gcgcagaaaa aaaggatctc aagaagatcc
     3721 tttgatcttt tctacggggt ctgacgctca gtggaacgaa aactcacgtt aagggatttt
     3781 ggtcatgaat tcttgttaga cattatttgc cgactacctt ggtgatctcg cctttcacgt
     3841 agtgaacaaa ttcttccaac tgatctgcgc gcgaggccaa gcgatcttct tgtccaagat
     3901 aagcctgcct agcttcaagt atgacgggct gatactgggc cggcaggcgc tccattgccc
     3961 agtcggcagc gacatccttc ggcgcgattt tgccggttac tgcgctgtac caaatgcggg
     4021 acaacgtaag cactacattt cgctcatcgc cagcccagtc gggcggcgag ttccatagcg
     4081 ttaaggtttc atttagcgcc tcaaatagat cctgttcagg aaccggatca aagagttcct
     4141 ccgccgctgg acctaccaag gcaacgctat gttctcttgc ttttgtcagc aagatagcca
     4201 gatcaatgtc gatcgtggct ggctcgaaga tacctgcaag aatgtcattg cgctgccatt
     4261 ctccaaattg cagttcgcgc ttagctggat aacgccacgg aatgatgtcg tcgtgcacaa
     4321 caatggtgac ttctacagcg cggagaatct cgctctctcc aggggaagcc gaagtttcca
     4381 aaaggtcgtt gatcaaagct cgccgcgttg tttcatcaag ccttacggtc accgtaacca
     4441 gcaaatcaat atcactgtgt ggcttcaggc cgccatccac tgcggagccg tacaaatgta
     4501 cggccagcaa cgtcggttcg agatggcgct cgatgacgcc aactacctct gatagttgag
     4561 tcgatacttc ggcgatcacc gcttccctca tgatgtttaa ctcctgaatt aagccgcgcc
     4621 gcgaagcggt gtCggcttga atgaattgtt aggcgtcatc ctgtgctccc gagaaccagt
     4681 accagtacat cgctgtttcg ttcgagactt gaggtctagt tttatacgtg aacaggtcaa
     4741 tgccgccgag agtaaagcca caTtttgcgt acaaattgca ggcaggtaca ttgttcgttt
     4801 gtgCCATGAT GATATATTTT TATCTTGTGC AATGTAACAT CAGAGATTTT GAGACACAAC
     4861 GTGGCTTTGT TGAATAAATC GAACTTTTGC TGAGTTGAAG GATCAGATCA CGCATCTTCC
     4921 CGACAACGCA GACCGTTCCG TGGCAAAGCA AAAGTTCAAA ATCACCAACT GGTCCACCTA
     4981 CAACAAAGCT CTCATCAACC GTGGCTCCCT CACTTTCTGG CTGGATGATG GGGCGATTCA
     5041 GGCAGATCCC CATCCAACAG CCCGCCGTCG AGCGGGCTTT TTTATCCCCG GAAGCCTGTG
     5101 GATAGAGGGT AGTTATCCAC GTGAAACCGC TAATGCCCCG CAAAGCCTTG ATTCACGGGG
     5161 CTTTCCGGCC CGCTCCAAAA ACTATCCACG TGAAATCGCT AATCAGGGTA CGTGAAATCG
     5221 CTAATCGGAG TACGTGAAAT CGCTAATAAG GTCACGTGAA ATCGCTAATC AAAAAGGCAC
     5281 GTGAGAACGC TAATAGCCCT TTCAGATCAA CAGCTTGCAA ACACCCCTCG CTCCGGCAAG
     5341 TAGTTACAGC AAGTAGTATG TTCAATTAGC TTTTCAATTA TGAATATATA TATCAATTAT
     5401 TGGTCGCCCT TGGCTTGTGG ACAATGCGCT ACGCGCACCG GCTCCGCCCG TGGACAACCG
     5461 CAAGCGGTTG CCCACCGTCG AGCGCCTTTG CCCACAACCC GGCGGCCGGC CGCAACAGAT
     5521 CGTTTTATAA ATTTTTTTTT TTGAAAAAGA AAAAGCCCGA AAGGCGGCAA CCTCTCGGGC
     5581 TTCTGGATTT CCGATCCccg gaattagact ctggctgcct gtatcgagtg gtgattttgt
     5641 gccgagctgc cggtcgggga gctgttggct ggctgg
//