LOCUS       pJAB3603_proUBQ10::PIP2 7049 bp ds-DNA     circular     30-JUN-2022
DEFINITION  .
FEATURES             Location/Qualifiers
     misc_feature    1..24
                     /label="LB left border"
                     /ApEinfo_revcolor=#b1ff67
                     /ApEinfo_fwdcolor=#b1ff67
     misc_feature    34..674
                     /label="region used as proUBQ10 promoter (JANB)"
                     /ApEinfo_revcolor=#84b0dc
                     /ApEinfo_fwdcolor=#84b0dc
     misc_feature    369..672
                     /label="UBQ10 5' intron"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     misc_feature    675..678
                     /label="scar"
                     /ApEinfo_revcolor=#f8d3a9
                     /ApEinfo_fwdcolor=#f8d3a9
     CDS             679..1540
                     /label="PIP2A"
                     /ApEinfo_revcolor=#104aff
                     /ApEinfo_fwdcolor=#104aff
     CDS             1546..2256
                     /label="mCherry"
                     /ApEinfo_revcolor=#ff0000
                     /ApEinfo_fwdcolor=#ff0000
     misc_feature    1974..1974
                     /label="G->T mutation to remove Bbs1"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     terminator      2280..2529
                     /label="nos\3'"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     misc_feature    2544..2549
                     /label="Bbs1"
                     /ApEinfo_revcolor=#ff0000
                     /ApEinfo_fwdcolor=#ff0000
     misc_feature    2552..2555
                     /label="Bsa1 seam"
                     /ApEinfo_revcolor=#f8d3a9
                     /ApEinfo_fwdcolor=#f8d3a9
     misc_feature    2582..2611
                     /label="PhlFO"
                     /ApEinfo_revcolor=#008080
                     /ApEinfo_fwdcolor=#008080
     misc_feature    2614..2643
                     /label="PhlFO"
                     /ApEinfo_revcolor=#008080
                     /ApEinfo_fwdcolor=#008080
     misc_feature    2646..2675
                     /label="PhlFO"
                     /ApEinfo_revcolor=#008080
                     /ApEinfo_fwdcolor=#008080
     misc_feature    2678..2707
                     /label="PhlFO"
                     /ApEinfo_revcolor=#008080
                     /ApEinfo_fwdcolor=#008080
     misc_feature    2715..2715
                     /label="C ->A mutation to remove Bsa1"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     misc_feature    2728..2731
                     /label="Bsa1 seam"
                     /ApEinfo_revcolor=#f8d3a9
                     /ApEinfo_fwdcolor=#f8d3a9
     misc_feature    2732..2737
                     /label="CACAAT box"
                     /ApEinfo_revcolor=#008000
                     /ApEinfo_fwdcolor=#008000
     misc_feature    2732..2814
                     /label="min35S"
                     /ApEinfo_revcolor=#b1ff67
                     /ApEinfo_fwdcolor=#b1ff67
     misc_feature    2739..2743
                     /label="CCAAT box"
                     /ApEinfo_revcolor=#008000
                     /ApEinfo_fwdcolor=#008000
     misc_feature    2767..2773
                     /label="TATA extended"
                     /ApEinfo_revcolor=#00bb00
                     /ApEinfo_fwdcolor=#00bb00
     misc_feature    2797..2797
                     /label="TSS should be a 'G'"
                     /ApEinfo_revcolor=#008000
                     /ApEinfo_fwdcolor=#008000
     misc_feature    2815..2818
                     /label="Bsa1 seam"
                     /ApEinfo_revcolor=#f8d3a9
                     /ApEinfo_fwdcolor=#f8d3a9
     misc_feature    2819..3019
                     /label="mGFP6"
                     /ApEinfo_revcolor=#b1ff67
                     /ApEinfo_fwdcolor=#b1ff67
     misc_feature    3020..3208
                     /label="ST-LS1 intron2"
                     /ApEinfo_revcolor=#8493ca
                     /ApEinfo_fwdcolor=#8493ca
     misc_feature    3209..3727
                     /label="mGFP6"
                     /ApEinfo_revcolor=#b1ff67
                     /ApEinfo_fwdcolor=#b1ff67
     misc_feature    3358..3358
                     /label="A->T mutation to remove Bbs1"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     misc_feature    3728..3731
                     /label="Bsa1 seam"
                     /ApEinfo_revcolor=#f8d3a9
                     /ApEinfo_fwdcolor=#f8d3a9
     misc_feature    3732..3982
                     /label="ADH terminator"
                     /ApEinfo_revcolor=#d59687
                     /ApEinfo_fwdcolor=#d59687
     misc_feature    3983..3986
                     /label="Bsa1 seam"
                     /ApEinfo_revcolor=#f8d3a9
                     /ApEinfo_fwdcolor=#f8d3a9
     misc_feature    complement(3989..3994)
                     /label="Bbs1"
                     /ApEinfo_revcolor=#ff0000
                     /ApEinfo_fwdcolor=#ff0000
     primer_bind     complement(4053..4072)
                     /label="T3"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     primer_bind     complement(4090..4110)
                     /label="M13-rev"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     misc_feature    4447..4495
                     /label="RB right border"
                     /ApEinfo_revcolor=#b1ff67
                     /ApEinfo_fwdcolor=#b1ff67
     misc_feature    4544..5225
                     /label="colE1"
                     /ApEinfo_revcolor=#7f7f7f
                     /ApEinfo_fwdcolor=#7f7f7f
     misc_marker     complement(5244..6029)
                     /label="Spec-R"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     misc_feature    complement(6030..6241)
                     /label="spec promoter"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     misc_feature    6481..6481
                     /label="C->A mutation"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     misc_feature    6482..7034
                     /label="pSa ori"
                     /ApEinfo_revcolor=#84b0dc
                     /ApEinfo_fwdcolor=#84b0dc
ORIGIN
        1 tggcaggata tattgtggtg taacgttAAG CTTTGGTTGT CGACGAGTCA GTAATAAACG
       61 GCGTCAAAGT GGTTGCAGCC GGCACACACG AGTCGTGTTT ATCAACTCAA AGCACAAATA
      121 CTTTTCCTCA ACCTAAAAAT AAGGCAATTA GCCAAAAACA ACTTTGCGTG TAAACAACGC
      181 TCAATACACG TGTCATTTTA TTATTAGCTA TTGCTTCACC GCCTTAGCTT TCTCGTGACC
      241 TAGTCGTCCT CGTCTTTTCT TCTTCTTCTT CTATAAAACA ATACCCAAAG AGCTCTTCTT
      301 CTTCACAATT CAGATTTCAA TTTCTCAAAA TCTTAAAAAC TTTCTCTCAA TTCTCTCTAC
      361 CGTGATCAAG GTAAATTTCT GTGTTCCTTA TTCTCTCAAA ATCTTCGATT TTGTTTTCGT
      421 TCGATCCCAA TTTCGTATAT GTTCTTTGGT TTAGATTCTG TTAATCTTAG ATCGTAGACG
      481 ATTTTCTGGG TTTGATCGTT AGATATCATC TTAATTCTCG ATTAGGGTTT CATAGATATC
      541 ATCCGATTTG TTCAAATAAT TTGAGTTTTG TCGAATAATT ACTCTTCGAT TTGTGATTTC
      601 TATCTAGATC TGGTGTTAGT TTCTAGTTTG TGCGATCGAA TTTGTCGATT AATCTGAGTT
      661 TTTCTGATTA ACAGCACCat ggcaaaggat gtggaagccg ttcccggaga aggatttcag
      721 acaagagact atcaagatcc gccaccagct ccgtttattg atggagcgga gctaaagaag
      781 tggtctttct acagagcagt tatcgcAgag ttcgtagcca ctctcctctt cttatacatc
      841 accgttttga cagtcatcgg ttacaagatt cagtccgata ctgatgccgg tggCgtagat
      901 tgcggcggag ttggaatcct cggtatcgct tgggcctttg gtggtatgat cttcatcctc
      961 gtctactgca ccgccggtat ctctggtggt cacattaacc cagcggtgac atttgggcta
     1021 ttcttggcac gtaaagtgtc gttacctagg gccctattgt acataatcgc tcagtgtttg
     1081 ggtgcgattt gtggagttgg ttttgtcaaa gccttccaaa gctcttacta cacccgttac
     1141 ggaggtggag ccaactctct agccgatggc tacagcacag ggaccggtCt agccgcagag
     1201 atcattggta ctttcgttct tgtctacacC gtAttctctg cCactgaccc caaacgtagt
     1261 gcCagagact cccacgttcc ggtgttggcg ccacttccaa tcggatttgc cgtgttcatg
     1321 gtacatttgg ctaccattcc cattaccgga accggaatta acccggcaag gagtttcgga
     1381 gctgccgtaa tctacaacaa gagcaagcca tgggatgacc actggatatt ttgggttgga
     1441 ccattcattg gagctgcgat agctgcattc taccaccaat tcgttctAag agcttcaggt
     1501 tctaagtctc tTggatcatt cagaagtgct gccaacgtcg gatccatggt gagcaagggc
     1561 gaggaggata acatggccat catcaaggag ttcatgcgct tcaaggtgca catggagggc
     1621 tccgtgaacg gccacgagtt cgagatcgag ggcgagggcg agggccgccc ctacgagggc
     1681 acccagaccg ccaagctgaa ggtgaccaag ggtggccccc tgcccttcgc ctgggacatc
     1741 ctgtcccctc agttcatgta cggctccaag gcctacgtga agcaccccgc cgacatcccc
     1801 gactacttga agctgtcctt ccccgagggc ttcaagtggg agcgcgtgat gaacttcgag
     1861 gacggcggcg tggtgaccgt gacccaggac tcctccctgc aggacggcga gttcatctac
     1921 aaggtgaagc tgcgcggcac caacttcccc tccgacggcc ccgtaatgca gaaTAAGACc
     1981 atgggctggg aggcctcctc cgagcggatg taccccgagg acggcgccct gaagggcgag
     2041 atcaagcaga ggctgaagct gaaggacggc ggccactacg acgctgaggt caagaccacc
     2101 tacaaggcca agaagcccgt gcagctgccc ggcgcctaca acgtcaacat caagttggac
     2161 atcacctccc acaacgagga ctacaccatc gtggaacagt acgaacgcgc cgagggccgc
     2221 cactccaccg gcggcatgga Ggagctgtac AAGtaaagcg gccgcccggc tgcaGATCgt
     2281 tcaaacattt ggcaataaag tttcttaaga ttgaatcctg ttgccggtct tgcgatgatt
     2341 atcatataat ttctgttgaa ttacgttaag catgtaataa ttaacatgta atgcatgacg
     2401 ttatttatga gatgggtttt tatgattaga gtcccgcaat tatacattta atacgcgata
     2461 gaaaacaaaa tatagcgcgc aaactaggat aaattatcgc gcgcggtgtc atctatgtta
     2521 ctagatcggg gcgcgccctc gagGAAGACg taatgATAGA AAAGTTGGCT GCGGCCGCGA
     2581 AATGATACGA AACGTACCGT ATCGTTAAGG TCAATGATAC GAAACGTACC GTATCGTTAA
     2641 GGTGTATGAT ACGAAACGTA CCGTATCGTT AAGGTAGATG ATACGAAACG TACCGTATCG
     2701 TTAAGGTCGG GTCTAAGCTA GCTAGGCCAC CCACAATcCC ACTatccttt cgcaagaccc
     2761 ttcctcTATA TAAggaagtt catttcattt ggagagAaca cgggggactc tagaCACCAT
     2821 GGTGAGTAAA GGAGAAGAAC TTTTCACTGG AGTTGTCCCA ATTCTTGTTG AATTAGATGG
     2881 TGATGTTAAT GGGCACAAAT TTTCTGTCAG TGGAGAGGGT GAAGGTGATG CAACATACGG
     2941 AAAACTTACC CTTAAATTTA TTTGCACTAC TGGAAAACTA CCTGTTCCAT GGCCAACCCT
     3001 GGTCACCACC CTGACCTACg taagtttctg cttctacctt tgatatatat ataataatta
     3061 tcattaatta gtagtaatat aatatttcaa atattttttt caaaataaaa gaatgtagta
     3121 tatagcaatt gcttttctgt agtttataag tgtgtatatt ttaatttata acttttctaa
     3181 tatatgacca aaatttgttg atgtgcagGG CGTGCAGTGC TTCTCCCGTT ACCCTGATCA
     3241 TATGAAGCGG CACGACTTCT TCAAGAGCGC CATGCCTGAG GGATACGTGC AGGAGAGGAC
     3301 CATCTTCTTC AAGGACGACG GGAACTACAA GACACGTGCT GAAGTCAAGT TTGAGGGTGA
     3361 CACCCTCGTC AACAGGATCG AGCTTAAGGG AATCGATTTC AAGGAGGACG GAAACATCCT
     3421 CGGCCACAAG TTGGAATACA ACTACAACTC CCACAACGTA TACATCATGG CCGACAAGCA
     3481 AAAGAACGGC ATCAAAGCCA ACTTCAAGAC CCGCCACAAC ATCGAGGACG GCGGCGTGCA
     3541 ACTCGCTGAT CATTATCAAC AAAATACTCC AATTGGCGAT GGCCCTGTCC TTTTACCAGA
     3601 CAACCATTAC CTGTCCACAC AATCTGCCCT TTCGAAAGAT CCCAACGAAA AGAGAGATCA
     3661 CATGGTCCTT CTTGAGTTTG TAACAGCTGC TGGGATTACA CATGGCATGG ATGAACTATA
     3721 CAAATAAtgt caagccattc tctcgcagat gatgttcact ttgtgtttta cttcctttat
     3781 gcattcacag caataaaaga aagaaatctc catcgctttt ggttttcttc tctgtcttaa
     3841 gttagtcgtt ttcgtgtcta atctattact tatcattgta atagactctt cttctattga
     3901 gatttgaata taaactaaaa cacattccat tttactgtgt tctcaacatt cagaatgcaa
     3961 acggactaac cgtagtgact cggcttgaGT CTTCgggcta gagcggacta gttctagagc
     4021 ggccgccacc gcggtggagc tccagctttt gttcccttta gtgagggtta attgcgcgct
     4081 tggcgtaatc atggtcatag ctgtttcctg tgtgaaattg ttatccgctc acaattccac
     4141 acaacatacg agccggaagc ataaagtgta aagcctgggg tgcctaatga gtgagctaac
     4201 tcacattaat tgcgttgcgc tcactgcccg ctttccagtc gggaaacctg tcgtgccagc
     4261 tgcattaatg aatcggccaa cgcgcgggga gaggcggttt gcgtattggg cgctcttccg
     4321 cttcctcgct cactgactcg ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc
     4381 actcaaaggc ggtaatacgg ttatccacag aatcagggga taacgcagga aagaacatga
     4441 aggccttgac aggatatatt ggcgggtaaa ctaagtcgct gtatgtgttt gtttgagatc
     4501 tcatgtgagc aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt
     4561 ttttccatag gctccgcccc cctgacgagc atcacaaaaa tcgacgctca agtcagaggt
     4621 ggcgaaaccc gacaggacta taaagatacc aggcgtttcc ccctggaagc tccctcgtgc
     4681 gctctcctgt tccgaccctg ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa
     4741 gcgtggcgct ttctcatagc tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct
     4801 ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc ttatccggta
     4861 actatcgtct tgagtccaac ccggtaagac acgacttatc gccactggca gcagccactg
     4921 gtaacaggat tagcagagcg aggtatgtag gcggtgctac agagttcttg aagtggtggc
     4981 ctaactacgg ctacactaga agaacagtat ttggtatctg Tgctctgctg aagccagtta
     5041 ccttcggaag aagagttggt agctcttgat ccggcaaaca aaccaccgct ggtagcggtg
     5101 gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa gaagatcctt
     5161 tgatcttttc tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg
     5221 tcatgaattc ttgttagaca ttatttgccg actaccttgg tgatctcgcc tttcacgtag
     5281 tgaacaaatt cttccaactg atctgcgcgc gaggccaagc gatcttcttg tccaagataa
     5341 gcctgcctag cttcaagtat gacgggctga tactgggccg gcaggcgctc cattgcccag
     5401 tcggcagcga catccttcgg cgcgattttg ccggttactg cgctgtacca aatgcgggac
     5461 aacgtaagca ctacatttcg ctcatcgcca gcccagtcgg gcggcgagtt ccatagcgtt
     5521 aaggtttcat ttagcgcctc aaatagatcc tgttcaggaa ccggatcaaa gagttcctcc
     5581 gccgctggac ctaccaaggc aacgctatgt tctcttgctt ttgtcagcaa gatagccaga
     5641 tcaatgtcga tcgtggctgg ctcgaagata cctgcaagaa tgtcattgcg ctgccattct
     5701 ccaaattgca gttcgcgctt agctggataa cgccacggaa tgatgtcgtc gtgcacaaca
     5761 atggtgactt ctacagcgcg gagaatctcg ctctctccag gggaagccga agtttccaaa
     5821 aggtcgttga tcaaagctcg ccgcgttgtt tcatcaagcc ttacggtcac cgtaaccagc
     5881 aaatcaatat cactgtgtgg cttcaggccg ccatccactg cggagccgta caaatgtacg
     5941 gccagcaacg tcggttcgag atggcgctcg atgacgccaa ctacctctga tagttgagtc
     6001 gatacttcgg cgatcaccgc ttccctcatg atgtttaact cctgaattaa gccgcgccgc
     6061 gaagcggtgt Cggcttgaat gaattgttag gcgtcatcct gtgctcccga gaaccagtac
     6121 cagtacatcg ctgtttcgtt cgagacttga ggtctagttt tatacgtgaa caggtcaatg
     6181 ccgccgagag taaagccaca Ttttgcgtac aaattgcagg caggtacatt gttcgtttgt
     6241 gCCATGATGA TATATTTTTA TCTTGTGCAA TGTAACATCA GAGATTTTGA GACACAACGT
     6301 GGCTTTGTTG AATAAATCGA ACTTTTGCTG AGTTGAAGGA TCAGATCACG CATCTTCCCG
     6361 ACAACGCAGA CCGTTCCGTG GCAAAGCAAA AGTTCAAAAT CACCAACTGG TCCACCTACA
     6421 ACAAAGCTCT CATCAACCGT GGCTCCCTCA CTTTCTGGCT GGATGATGGG GCGATTCAGG
     6481 AGATCCCCAT CCAACAGCCC GCCGTCGAGC GGGCTTTTTT ATCCCCGGAA GCCTGTGGAT
     6541 AGAGGGTAGT TATCCACGTG AAACCGCTAA TGCCCCGCAA AGCCTTGATT CACGGGGCTT
     6601 TCCGGCCCGC TCCAAAAACT ATCCACGTGA AATCGCTAAT CAGGGTACGT GAAATCGCTA
     6661 ATCGGAGTAC GTGAAATCGC TAATAAGGTC ACGTGAAATC GCTAATCAAA AAGGCACGTG
     6721 AGAACGCTAA TAGCCCTTTC AGATCAACAG CTTGCAAACA CCCCTCGCTC CGGCAAGTAG
     6781 TTACAGCAAG TAGTATGTTC AATTAGCTTT TCAATTATGA ATATATATAT CAATTATTGG
     6841 TCGCCCTTGG CTTGTGGACA ATGCGCTACG CGCACCGGCT CCGCCCGTGG ACAACCGCAA
     6901 GCGGTTGCCC ACCGTCGAGC GCCTTTGCCC ACAACCCGGC GGCCGGCCGC AACAGATCGT
     6961 TTTATAAATT TTTTTTTTTG AAAAAGAAAA AGCCCGAAAG GCGGCAACCT CTCGGGCTTC
     7021 TGGATTTCCG ATCCccggaa ttagactct
//