LOCUS       pJAB3616_proUBQ10::PIP2 8923 bp ds-DNA     circular     30-JUN-2022
DEFINITION  .
FEATURES             Location/Qualifiers
     misc_feature    1..24
                     /label="LB left border"
                     /ApEinfo_revcolor=#b1ff67
                     /ApEinfo_fwdcolor=#b1ff67
     misc_feature    34..674
                     /label="region used as proUBQ10 promoter (JANB)"
                     /ApEinfo_revcolor=#84b0dc
                     /ApEinfo_fwdcolor=#84b0dc
     misc_feature    369..672
                     /label="UBQ10 5' intron"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     misc_feature    675..678
                     /label="scar"
                     /ApEinfo_revcolor=#f8d3a9
                     /ApEinfo_fwdcolor=#f8d3a9
     CDS             679..1540
                     /label="PIP2A"
                     /ApEinfo_revcolor=#104aff
                     /ApEinfo_fwdcolor=#104aff
     CDS             1546..2256
                     /label="mCherry"
                     /ApEinfo_revcolor=#ff0000
                     /ApEinfo_fwdcolor=#ff0000
     misc_feature    1974..1974
                     /label="G->T mutation to remove Bbs1"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     terminator      2280..2529
                     /label="nos\3'"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     misc_feature    2544..2549
                     /label="Bsa1"
                     /ApEinfo_revcolor=#ff0000
                     /ApEinfo_fwdcolor=#ff0000
     misc_feature    2551..2554
                     /label="Bsa1 seam"
                     /ApEinfo_revcolor=#f8d3a9
                     /ApEinfo_fwdcolor=#f8d3a9
     misc_feature    2555..3368
                     /label="35S promoter"
                     /ApEinfo_revcolor=#b7e6d7
                     /ApEinfo_fwdcolor=#b7e6d7
     misc_feature    2628..2628
                     /label="G->C mutation to remove Bsa1"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     primer          3283..3310
                     /label="sJAB064"
                     /note="sequence: gcaagtggattgatgtgatatctccact"
                     /ApEinfo_revcolor=#b7e6d7
                     /ApEinfo_fwdcolor=#b7e6d7
     misc_feature    3309..3329
                     /label="as-1 (Lam et al, 1989)"
                     /ApEinfo_revcolor=#213e1f
                     /ApEinfo_fwdcolor=#213e1f
     misc_feature    3330..3332
                     /label="CACAAT box P35S"
                     /ApEinfo_revcolor=#00ae00
                     /ApEinfo_fwdcolor=#00ae00
     misc_feature    3352..3359
                     /label="35S mutation to increase expression?"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     misc_feature    3362..3368
                     /label="TATA extended"
                     /ApEinfo_revcolor=#00bb00
                     /ApEinfo_fwdcolor=#00bb00
     misc_feature    3369..3387
                     /label="tetO (Triple-Op, Gatz 1992)"
                     /ApEinfo_revcolor=#84b0dc
                     /ApEinfo_fwdcolor=#84b0dc
     misc_feature    3388..3411
                     /label="35S promoter"
                     /ApEinfo_revcolor=#b7e6d7
                     /ApEinfo_fwdcolor=#b7e6d7
     misc_feature    3394..3394
                     /label="+1 P35S"
                     /ApEinfo_revcolor=#00c500
                     /ApEinfo_fwdcolor=#00c500
     misc_feature    3412..3415
                     /label="Bsa1 seam"
                     /ApEinfo_revcolor=#f8d3a9
                     /ApEinfo_fwdcolor=#f8d3a9
     misc_feature    3416..3616
                     /label="mGFP6"
                     /ApEinfo_revcolor=#b1ff67
                     /ApEinfo_fwdcolor=#b1ff67
     misc_feature    3617..3805
                     /label="ST-LS1 intron2"
                     /ApEinfo_revcolor=#8493ca
                     /ApEinfo_fwdcolor=#8493ca
     misc_feature    3806..4324
                     /label="mGFP6"
                     /ApEinfo_revcolor=#b1ff67
                     /ApEinfo_fwdcolor=#b1ff67
     misc_feature    3955..3955
                     /label="A->T mutation to remove Bbs1"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     misc_feature    4325..4328
                     /label="Bsa1 seam"
                     /ApEinfo_revcolor=#f8d3a9
                     /ApEinfo_fwdcolor=#f8d3a9
     misc_feature    4329..4579
                     /label="ADH terminator"
                     /ApEinfo_revcolor=#d59687
                     /ApEinfo_fwdcolor=#d59687
     misc_feature    4580..4583
                     /label="Bsa1 seam"
                     /ApEinfo_revcolor=#f8d3a9
                     /ApEinfo_fwdcolor=#f8d3a9
     terminator      complement(4584..4808)
                     /label="19STer"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     misc_feature    complement(4812..4815)
                     /label="Bsa1 seam"
                     /ApEinfo_revcolor=#f8d3a9
                     /ApEinfo_fwdcolor=#f8d3a9
     misc_feature    complement(4816..4818)
                     /label="stop"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     misc_feature    complement(4819..4839)
                     /label="SV40 NLS"
                     /ApEinfo_revcolor=#84b0dc
                     /ApEinfo_fwdcolor=#84b0dc
     misc_feature    complement(4840..5460)
                     /label="TetR"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     primer          complement(4974..5001)
                     /label="sJAB151"
                     /note="sequence: gccaaagaggaaagagagacacccacca"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     misc_feature    complement(5461..5464)
                     /label="Bsa1 seam"
                     /ApEinfo_revcolor=#f8d3a9
                     /ApEinfo_fwdcolor=#f8d3a9
     misc_feature    complement(5465..5494)
                     /label="PhlFO"
                     /ApEinfo_revcolor=#008080
                     /ApEinfo_fwdcolor=#008080
     misc_feature    complement(5494..5494)
                     /label="A->T mutation to remove ATG"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     misc_feature    complement(5495..5498)
                     /label="Bsa1 seam"
                     /ApEinfo_revcolor=#f8d3a9
                     /ApEinfo_fwdcolor=#f8d3a9
     misc_feature    complement(5499..5581)
                     /label="min35S"
                     /ApEinfo_revcolor=#b1ff67
                     /ApEinfo_fwdcolor=#b1ff67
     misc_feature    complement(5516..5516)
                     /label="TSS should be a 'G'"
                     /ApEinfo_revcolor=#008000
                     /ApEinfo_fwdcolor=#008000
     misc_feature    complement(5540..5546)
                     /label="TATA extended"
                     /ApEinfo_revcolor=#00bb00
                     /ApEinfo_fwdcolor=#00bb00
     misc_feature    complement(5570..5574)
                     /label="CCAAT box"
                     /ApEinfo_revcolor=#008000
                     /ApEinfo_fwdcolor=#008000
     misc_feature    complement(5576..5581)
                     /label="CACAAT box"
                     /ApEinfo_revcolor=#008000
                     /ApEinfo_fwdcolor=#008000
     misc_feature    complement(5582..5585)
                     /label="Bsa1 seam"
                     /ApEinfo_revcolor=#f8d3a9
                     /ApEinfo_fwdcolor=#f8d3a9
     misc_feature    complement(5586..5615)
                     /label="PhlFO"
                     /ApEinfo_revcolor=#008080
                     /ApEinfo_fwdcolor=#008080
     misc_feature    complement(5616..5619)
                     /label="Bsa1 seam"
                     /ApEinfo_revcolor=#f8d3a9
                     /ApEinfo_fwdcolor=#f8d3a9
     misc_feature    complement(5644..5664)
                     /label="AmtRO"
                     /ApEinfo_revcolor=#008080
                     /ApEinfo_fwdcolor=#008080
     misc_feature    complement(5668..5688)
                     /label="AmtRO"
                     /ApEinfo_revcolor=#008080
                     /ApEinfo_fwdcolor=#008080
     misc_feature    complement(5692..5712)
                     /label="AmtRO"
                     /ApEinfo_revcolor=#008080
                     /ApEinfo_fwdcolor=#008080
     misc_feature    complement(5716..5736)
                     /label="AmtRO"
                     /ApEinfo_revcolor=#008080
                     /ApEinfo_fwdcolor=#008080
     misc_feature    complement(5740..5760)
                     /label="AmtRO"
                     /ApEinfo_revcolor=#008080
                     /ApEinfo_fwdcolor=#008080
     misc_feature    complement(5764..5784)
                     /label="AmtRO"
                     /ApEinfo_revcolor=#008080
                     /ApEinfo_fwdcolor=#008080
     misc_feature    5812..5815
                     /label="Bsa1 seam"
                     /ApEinfo_revcolor=#f8d3a9
                     /ApEinfo_fwdcolor=#f8d3a9
     misc_feature    5817..5820
                     /label="Bsa1 seam"
                     /ApEinfo_revcolor=#f8d3a9
                     /ApEinfo_fwdcolor=#f8d3a9
     misc_feature    complement(5822..5827)
                     /label="Bsa1"
                     /ApEinfo_revcolor=#ff0000
                     /ApEinfo_fwdcolor=#ff0000
     primer_bind     complement(5886..5905)
                     /label="T3"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     primer_bind     complement(5923..5943)
                     /label="M13-rev"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     misc_feature    6280..6328
                     /label="RB right border"
                     /ApEinfo_revcolor=#b1ff67
                     /ApEinfo_fwdcolor=#b1ff67
     misc_feature    6377..7058
                     /label="colE1"
                     /ApEinfo_revcolor=#7f7f7f
                     /ApEinfo_fwdcolor=#7f7f7f
     misc_feature    7070..7072
                     /label="kanR stop codon"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     CDS             complement(7073..7864)
                     /label="kanamycin (R)"
                     /ApEinfo_revcolor=#1e5bff
                     /ApEinfo_fwdcolor=#1e5bff
     misc_feature    complement(7289..7289)
                     /label="A->T mutation to remove Bbs1 site"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     misc_feature    complement(7379..7379)
                     /label="C->T mutation to remove Bbs1 site"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     misc_feature    complement(7784..7784)
                     /label="T->A mutation to remove Bbs1 site"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     misc_feature    complement(7865..8136)
                     /label="kanR promoter"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     misc_feature    8355..8355
                     /label="C->A mutation"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     misc_feature    8356..8908
                     /label="pSa ori"
                     /ApEinfo_revcolor=#84b0dc
                     /ApEinfo_fwdcolor=#84b0dc
ORIGIN
        1 tggcaggata tattgtggtg taacgttAAG CTTTGGTTGT CGACGAGTCA GTAATAAACG
       61 GCGTCAAAGT GGTTGCAGCC GGCACACACG AGTCGTGTTT ATCAACTCAA AGCACAAATA
      121 CTTTTCCTCA ACCTAAAAAT AAGGCAATTA GCCAAAAACA ACTTTGCGTG TAAACAACGC
      181 TCAATACACG TGTCATTTTA TTATTAGCTA TTGCTTCACC GCCTTAGCTT TCTCGTGACC
      241 TAGTCGTCCT CGTCTTTTCT TCTTCTTCTT CTATAAAACA ATACCCAAAG AGCTCTTCTT
      301 CTTCACAATT CAGATTTCAA TTTCTCAAAA TCTTAAAAAC TTTCTCTCAA TTCTCTCTAC
      361 CGTGATCAAG GTAAATTTCT GTGTTCCTTA TTCTCTCAAA ATCTTCGATT TTGTTTTCGT
      421 TCGATCCCAA TTTCGTATAT GTTCTTTGGT TTAGATTCTG TTAATCTTAG ATCGTAGACG
      481 ATTTTCTGGG TTTGATCGTT AGATATCATC TTAATTCTCG ATTAGGGTTT CATAGATATC
      541 ATCCGATTTG TTCAAATAAT TTGAGTTTTG TCGAATAATT ACTCTTCGAT TTGTGATTTC
      601 TATCTAGATC TGGTGTTAGT TTCTAGTTTG TGCGATCGAA TTTGTCGATT AATCTGAGTT
      661 TTTCTGATTA ACAGCACCat ggcaaaggat gtggaagccg ttcccggaga aggatttcag
      721 acaagagact atcaagatcc gccaccagct ccgtttattg atggagcgga gctaaagaag
      781 tggtctttct acagagcagt tatcgcAgag ttcgtagcca ctctcctctt cttatacatc
      841 accgttttga cagtcatcgg ttacaagatt cagtccgata ctgatgccgg tggCgtagat
      901 tgcggcggag ttggaatcct cggtatcgct tgggcctttg gtggtatgat cttcatcctc
      961 gtctactgca ccgccggtat ctctggtggt cacattaacc cagcggtgac atttgggcta
     1021 ttcttggcac gtaaagtgtc gttacctagg gccctattgt acataatcgc tcagtgtttg
     1081 ggtgcgattt gtggagttgg ttttgtcaaa gccttccaaa gctcttacta cacccgttac
     1141 ggaggtggag ccaactctct agccgatggc tacagcacag ggaccggtCt agccgcagag
     1201 atcattggta ctttcgttct tgtctacacC gtAttctctg cCactgaccc caaacgtagt
     1261 gcCagagact cccacgttcc ggtgttggcg ccacttccaa tcggatttgc cgtgttcatg
     1321 gtacatttgg ctaccattcc cattaccgga accggaatta acccggcaag gagtttcgga
     1381 gctgccgtaa tctacaacaa gagcaagcca tgggatgacc actggatatt ttgggttgga
     1441 ccattcattg gagctgcgat agctgcattc taccaccaat tcgttctAag agcttcaggt
     1501 tctaagtctc tTggatcatt cagaagtgct gccaacgtcg gatccatggt gagcaagggc
     1561 gaggaggata acatggccat catcaaggag ttcatgcgct tcaaggtgca catggagggc
     1621 tccgtgaacg gccacgagtt cgagatcgag ggcgagggcg agggccgccc ctacgagggc
     1681 acccagaccg ccaagctgaa ggtgaccaag ggtggccccc tgcccttcgc ctgggacatc
     1741 ctgtcccctc agttcatgta cggctccaag gcctacgtga agcaccccgc cgacatcccc
     1801 gactacttga agctgtcctt ccccgagggc ttcaagtggg agcgcgtgat gaacttcgag
     1861 gacggcggcg tggtgaccgt gacccaggac tcctccctgc aggacggcga gttcatctac
     1921 aaggtgaagc tgcgcggcac caacttcccc tccgacggcc ccgtaatgca gaaTAAGACc
     1981 atgggctggg aggcctcctc cgagcggatg taccccgagg acggcgccct gaagggcgag
     2041 atcaagcaga ggctgaagct gaaggacggc ggccactacg acgctgaggt caagaccacc
     2101 tacaaggcca agaagcccgt gcagctgccc ggcgcctaca acgtcaacat caagttggac
     2161 atcacctccc acaacgagga ctacaccatc gtggaacagt acgaacgcgc cgagggccgc
     2221 cactccaccg gcggcatgga Ggagctgtac AAGtaaagcg gccgcccggc tgcaGATCgt
     2281 tcaaacattt ggcaataaag tttcttaaga ttgaatcctg ttgccggtct tgcgatgatt
     2341 atcatataat ttctgttgaa ttacgttaag catgtaataa ttaacatgta atgcatgacg
     2401 ttatttatga gatgggtttt tatgattaga gtcccgcaat tatacattta atacgcgata
     2461 gaaaacaaaa tatagcgcgc aaactaggat aaattatcgc gcgcggtgtc atctatgtta
     2521 ctagatcggg gcgcgccctc gagGGTCTCt aatgGGTCCC CAGATTTGCC TTTTCAATTT
     2581 CAGAAAGAAT GCTAACCCAC AGATGGTTAG AGAGGCTTAC GCAGCAGCTC TCATCAAGAC
     2641 GATCTACCCG AGCAATAATC TCCAGGAAAT CAAATACCTT CCCAAGAAGG TTAAAGATGC
     2701 AGTCAAAAGA TTCAGGACTA ACTGCATCAA GAACACAGAG AAAGATATAT TTCTCAAGAT
     2761 CAGAAGTACT ATTCCAGTAT GGACGATTCA AGGCTTGCTT CACAAACCAA GGCAAGTAAT
     2821 AGAGATTGGA GTCTCTAAAA AGGTAGTTCC CACTGAATCA AAGGCCATGG AGTCAAAGAT
     2881 TCAAATAGAG GACCTAACAG AACTCGCCGT AAAGACTGGC GAACAGTTCA TACAGAGTCT
     2941 CTTACGACTC AATGACAAGA AGAAAATCTT CGTCAACATG GTGGAGCACG ACACACTTGT
     3001 CTACTCCAAA AATATCAAAG ATACAGTCTC AGAAGACCAA AGGGCAATTG AGACTTTTCA
     3061 ACAAAGGGTA ATATCCGGAA ACCTCCTCGG ATTCCATTGC CCAGCTATCT GTCACTTTAT
     3121 TGTGAAGATA GTGGAAAAGG AAGGTGGCTC CTACAAATGC CATCATTGCG ATAAAGGAAA
     3181 GGCCATCGTT GAAGATGCCT CTGCCGACAG TGGTCCCAAA GATGGACCCC CACCCACGAG
     3241 GAGCATCGTG GAAAAAGAAG ACGTTCCAAC CACGTCTTCA AAGCAAGTGG ATTGATGTGA
     3301 TATCTCCACT GACGTAAGGG ATGACGCACA ATCCCACTAT CCTTCGCAAG ATAGATAATT
     3361 CTATATAAAC TCTATCACTG ATAGAGTGGA GAGAACACGG GGGACTCTAG AcaccATGGT
     3421 GAGTAAAGGA GAAGAACTTT TCACTGGAGT TGTCCCAATT CTTGTTGAAT TAGATGGTGA
     3481 TGTTAATGGG CACAAATTTT CTGTCAGTGG AGAGGGTGAA GGTGATGCAA CATACGGAAA
     3541 ACTTACCCTT AAATTTATTT GCACTACTGG AAAACTACCT GTTCCATGGC CAACCCTGGT
     3601 CACCACCCTG ACCTACgtaa gtttctgctt ctacctttga tatatatata ataattatca
     3661 ttaattagta gtaatataat atttcaaata tttttttcaa aataaaagaa tgtagtatat
     3721 agcaattgct tttctgtagt ttataagtgt gtatatttta atttataact tttctaatat
     3781 atgaccaaaa tttgttgatg tgcagGGCGT GCAGTGCTTC TCCCGTTACC CTGATCATAT
     3841 GAAGCGGCAC GACTTCTTCA AGAGCGCCAT GCCTGAGGGA TACGTGCAGG AGAGGACCAT
     3901 CTTCTTCAAG GACGACGGGA ACTACAAGAC ACGTGCTGAA GTCAAGTTTG AGGGTGACAC
     3961 CCTCGTCAAC AGGATCGAGC TTAAGGGAAT CGATTTCAAG GAGGACGGAA ACATCCTCGG
     4021 CCACAAGTTG GAATACAACT ACAACTCCCA CAACGTATAC ATCATGGCCG ACAAGCAAAA
     4081 GAACGGCATC AAAGCCAACT TCAAGACCCG CCACAACATC GAGGACGGCG GCGTGCAACT
     4141 CGCTGATCAT TATCAACAAA ATACTCCAAT TGGCGATGGC CCTGTCCTTT TACCAGACAA
     4201 CCATTACCTG TCCACACAAT CTGCCCTTTC GAAAGATCCC AACGAAAAGA GAGATCACAT
     4261 GGTCCTTCTT GAGTTTGTAA CAGCTGCTGG GATTACACAT GGCATGGATG AACTATACAA
     4321 ATAATGTCaa gccattctct cgcagatgat gttcactttg tgttttactt cctttatgca
     4381 ttcacagcaa taaaagaaag aaatctccat cgcttttggt tttcttctct gtcttaagtt
     4441 agtcgttttc gtgtctaatc tattacttat cattgtaata gactcttctt ctattgagat
     4501 ttgaatataa actaaaacac attccatttt actgtgttct caacattcag aatgcaaacg
     4561 gactaaccgt agtgactcgg cttatcgatc tggattttag tactggattt tggttttagg
     4621 aattagaaat tttattgata gaagtatttt acaaatacaa atacatacta agggtttctt
     4681 atatgctcaa cacatgagcg aaaccctata agaaccctaa tttcccttat cgggaaacta
     4741 ctcacacatt atttatggag aaaatagaga gagatagatt tgtagagaga gactggtgat
     4801 ttcagcgtac cgacactaaa ccttcctctt cttcttcggG CTGCCGGACT CGCACTTCAG
     4861 CTGTTTTTCG AGGCCACAGA TGATCAGCTC GAGGCCGAAC AGAAAGGCGG GCTCGGCGCC
     4921 CTGATGATCG AACAGCTCAA TGGCCTGTCT CAGCAGAGGG GGCATGCTGT CGGTGGTGGG
     4981 TGTCTCTCTT TCCTCTTTGG CGACCTGGTG TTCCTGATCT TCCAGCACGC AGCCCAGTGT
     5041 AAAGTGGCCC ACGGCGCTCA GGGCGTACAG GGCATTTTCC AGGCTGAAGC CCTGCTGGCA
     5101 CAGGAAGGCC AGCTGGTTTT CCAGTGTCTC GTACTGCTTC TCGGTAGGTC TGGTGCCCAG
     5161 GTGCACTTTG GCGCCATCCC GGTGGGACAG CAGAGCGCAT CTGAAGCTCT TGGCGTTGTT
     5221 CCGCAGGAAA TCCTGCCAGC TCTCGCCTTC CAGGGGGCAA AAGTGGGTGT GGTGCCGGTC
     5281 CAGCATCTCG ATGGCCAGGG CGTCCAGCAG GGCTCTCTTG TTCTTCACGT GCCAGTACAG
     5341 GGTGGGCTGT TCCACGCCCA GTTTCTGAGC CAGCTTCCGG GTGGTCAGGC CCTCGATGCC
     5401 CACTTCGTTG AGCAGTTCCA GGGCGCTGTT GATCACTTTG CTCTTGTCCA GCCGGCTCAT
     5461 GGTGACCTTA ACGATACGGT ACGTTTCGTA TCAATTACtc tagagtcccc cgtgtTctct
     5521 ccaaatgaaa tgaacttccT TATATAgagg aagggtcttg cgaaaggatA GTGGgATTGT
     5581 GACCTACCTT AACGATACGG TACGTTTCGT ATCATGCTAG GTGGCCTAGC TAGCTTAGAC
     5641 CCGATTATCT ATAGATCGAT AGAAACTATT ATCTATAGAT CGATAGAAAA CATTATCTAT
     5701 AGATCGATAG AAATGATTAT CTATAGATCG ATAGAAATGA TTATCTATAG ATCGATAGAA
     5761 ATCATTATCT ATAGATCGAT AGAAATTCGC GGCCGCAGCC AACTTTTCTA TTACTcgctt
     5821 cGAGACCggg ctagagcgga ctagttctag agcggccgcc accgcggtgg agctccagct
     5881 tttgttccct ttagtgaggg ttaattgcgc gcttggcgta atcatggtca tagctgtttc
     5941 ctgtgtgaaa ttgttatccg ctcacaattc cacacaacat acgagccgga agcataaagt
     6001 gtaaagcctg gggtgcctaa tgagtgagct aactcacatt aattgcgttg cgctcactgc
     6061 ccgctttcca gtcgggaaac ctgtcgtgcc agctgcatta atgaatcggc caacgcgcgg
     6121 ggagaggcgg tttgcgtatt gggcgctctt ccgcttcctc gctcactgac tcgctgcgct
     6181 cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa ggcggtaata cggttatcca
     6241 cagaatcagg ggataacgca ggaaagaaca tgaaggcctt gacaggatat attggcgggt
     6301 aaactaagtc gctgtatgtg tttgtttgag atctcatgtg agcaaaaggc cagcaaaagg
     6361 ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca taggctccgc ccccctgacg
     6421 agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga ctataaagat
     6481 accaggcgtt tccccctgga agctccctcg tgcgctctcc tgttccgacc ctgccgctta
     6541 ccggatacct gtccgccttt ctcccttcgg gaagcgtggc gctttctcat agctcacgct
     6601 gtaggtatct cagttcggtg taggtcgttc gctccaagct gggctgtgtg cacgaacccc
     6661 ccgttcagcc cgaccgctgc gccttatccg gtaactatcg tcttgagtcc aacccggtaa
     6721 gacacgactt atcgccactg gcagcagcca ctggtaacag gattagcaga gcgaggtatg
     6781 taggcggtgc tacagagttc ttgaagtggt ggcctaacta cggctacact agaagaacag
     6841 tatttggtat ctgTgctctg ctgaagccag ttaccttcgg aagaagagtt ggtagctctt
     6901 gatccggcaa acaaaccacc gctggtagcg gtggtttttt tgtttgcaag cagcagatta
     6961 cgcgcagaaa aaaaggatct caagaagatc ctttgatctt ttctacgggg tctgacgctc
     7021 agtggaacga aaactcacgt taagggattt tggtcatgca ttctaggtac taaaacaatt
     7081 catccagtaa aatataatat tttattttct cccaatcagg cttgatcccc agtaagtcaa
     7141 aaaatagctc gacatactgt tcttccccga tatcctccct gatcgaccgg acgcagaagg
     7201 caatgtcata ccacttgtcc gccctgccgc ttctcccaag atcaataaag ccacttactt
     7261 tgccatcttt cacaaagatg ttgctgtcAc ccaggtcgcc gtgggaaaag acaagttcct
     7321 cttcgggctt ttccgtcttt aaaaaatcat acagctcgcg cggatcttta aatggagtAt
     7381 cttcttccca gttttcgcaa tccacatcgg ccagatcgtt attcagtaag taatccaatt
     7441 cggctaagcg gctgtctaag ctattcgtat agggacaatc cgatatgtcg atggagtgaa
     7501 agagcctgat gcactccgca tacagctcga taatcttttc agggctttgt tcatcttcat
     7561 actcttccga gcaaaggacg ccatcggcct cactcatgag cagattgctc cagccatcat
     7621 gccgttcaaa gtgcaggacc tttggaacag gcagctttcc ttccagccat agcatcatgt
     7681 ccttttcccg ttccacatca taggtggtcc ctttataccg gctgtccgtc atttttaaat
     7741 ataggttttc attttctccc accagcttat ataccttagc aggTgacatt ccttccgtat
     7801 cttttacgca gcggtatttt tcgatcagtt ttttcaattc cggtgatatt ctcattttag
     7861 ccatttatta tttccttcct cttttctaca gtatttaaag ataccccaag aagctaatta
     7921 taacaagacg aactccaatt cactgttcct tgcattctaa aaccttaaat accagaaaac
     7981 agctttttca aagttgtttt caaagttggc gtataacata gtatcgacgg agccgatttt
     8041 gaaaccgcgg tgatcacagg cagcaacgct ctgtcatcgt tacaatcaac atgctaccct
     8101 ccgcgagatc atccgtgttt caaacccggc agcttaTTGT GCAATGTAAC ATCAGAGATT
     8161 TTGAGACACA ACGTGGCTTT GTTGAATAAA TCGAACTTTT GCTGAGTTGA AGGATCAGAT
     8221 CACGCATCTT CCCGACAACG CAGACCGTTC CGTGGCAAAG CAAAAGTTCA AAATCACCAA
     8281 CTGGTCCACC TACAACAAAG CTCTCATCAA CCGTGGCTCC CTCACTTTCT GGCTGGATGA
     8341 TGGGGCGATT CAGGAGATCC CCATCCAACA GCCCGCCGTC GAGCGGGCTT TTTTATCCCC
     8401 GGAAGCCTGT GGATAGAGGG TAGTTATCCA CGTGAAACCG CTAATGCCCC GCAAAGCCTT
     8461 GATTCACGGG GCTTTCCGGC CCGCTCCAAA AACTATCCAC GTGAAATCGC TAATCAGGGT
     8521 ACGTGAAATC GCTAATCGGA GTACGTGAAA TCGCTAATAA GGTCACGTGA AATCGCTAAT
     8581 CAAAAAGGCA CGTGAGAACG CTAATAGCCC TTTCAGATCA ACAGCTTGCA AACACCCCTC
     8641 GCTCCGGCAA GTAGTTACAG CAAGTAGTAT GTTCAATTAG CTTTTCAATT ATGAATATAT
     8701 ATATCAATTA TTGGTCGCCC TTGGCTTGTG GACAATGCGC TACGCGCACC GGCTCCGCCC
     8761 GTGGACAACC GCAAGCGGTT GCCCACCGTC GAGCGCCTTT GCCCACAACC CGGCGGCCGG
     8821 CCGCAACAGA TCGTTTTATA AATTTTTTTT TTTGAAAAAG AAAAAGCCCG AAAGGCGGCA
     8881 ACCTCTCGGG CTTCTGGATT TCCGATCCcc ggaattagac tct
//