LOCUS       Exported                6550 bp ds-DNA     circular UNA 15-NOV-2021
DEFINITION  natural circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 6550)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Aug 7, 2022 from SnapGene 4.2.4
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..6550
                     /organism="unspecified"
                     /mol_type="genomic DNA"
     unsure          1..141
                     /label=L-ITR
                     /note="/vntifkey=21"
     promoter        156..597
                     /label=synapsin
     misc_feature    519..536
                     /label=Forward primer region
                     /note="Forward primer region"
     primer_bind     569..597
                     /label=F_hSyn_IgK
     primer_bind     572..597
                     /label=c3 dio fw 4
     CDS             598..2652
                     /codon_start=1
                     /label=HIs2.0
                     /translation="METDTLLLWVLLLWVPGSTGDTSLYKKVGTTGMEPAGAGGAAILL
                     DDDDDYYLTTPGYPGSGSNASLSPPADLFPAASNHSTAVVVSGDLDLYVIIPLGIFLAV
                     LSLVTFAGNVMVLHALRTEKKLQTVSNIFIMSLAIADCSVGLFAMPLSAVYALLGYWPF
                     GLVVCNMWLSMDYVASTASIFNLFALSLDRFWSIISPLRYLKKRTRKRAIIMCSSVWLA
                     SLLWIIPVVGWHQMFNDGRRDVPVEECDTEFADEQVFKVLTAIFNFYVPMAAMIALYGR
                     IFHEIKLRAASQPAPAGPRDTDALDLEEGGNVYIKADKQKNGIKANFVIRHNIEDGGVQ
                     LAYHYQQNTPIGDGPVLLPDNHYLSVQSKLSKDPNEKRDHMVLLEFVTAAGITLGMDEL
                     YKGGTGGSMVRKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATEGKLTLKFICTT
                     GKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYIQERTIFFKDDGNYKTR
                     AEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNTGAAARWRAKKAAFQLGVILGAFC
                     LCFMPYFISFIITAFCKDCVSPAVHSIFTWLGYVNSTTNPFLYTWTNANFRSAFRKILR
                     MKETPKKPVYMQMPHKTPRPKKQPLEARVSGTASGGTTAPARKSDDTRL"
     primer_bind     complement(2653..2683)
                     /label=R_pAAV_AVP1.5
     unsure          2667..3275
                     /label=WPRE
                     /note="/vntifkey=21"
     primer_bind     complement(2752..2769)
                     /label=pWPTRev
     polyA_signal    3295..3773
                     /label=hGH polyA
                     /label=hGH polyA
                     /label=PolyA signal
                     /note="/vntifkey=25"
     unsure          3813..3953
                     /label=R-ITR
                     /note="/vntifkey=21"
     rep_origin      4045..4351
                     /label=f1 Origin
                     /label=f1 Origin
                     /label=Replication origin
                     /note="/vntifkey=33"
     unsure          4870..5727
                     /label=AmpR
                     /note="/vntifkey=21"
     rep_origin      5878..6545
                     /label=pUC ori
                     /label=pUC ori
                     /label=Replication origin
                     /note="/vntifkey=33"
ORIGIN
        1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcaaag cccgggcgtc
       61 gggcgacctt tggtcgcccg gcctcagtga gcgagcgagc gcgcagagag ggagtggcca
      121 actccatcac taggggttcc tgcggccgca cgcgtatgag gcggggtggg ggtgcctacc
      181 tgacgaccga ccccgaccca ctggacaagc acccaacccc cattccccaa attgcgcatc
      241 ccctatcaga gagggggagg ggaaacagga tgcggcgagg cgcgtgcgca ctgccagctt
      301 cagcaccgcg gacagtgcct tcgcccccgc ctggcggcgc gcgccaccgc cgcctcagca
      361 ctgaaggcgc gctgacgtca ctcgccggtc ccccgcaaac tccccttccc ggccaccttg
      421 gtcgcgtccg cgccgccgcc ggcccagccg gaccgcacca cgcgaggcgc gagatagggg
      481 ggcacgggcg cgaccatctg cgctgcggcg ccggcgactc agcgctgcct cagtctgcgg
      541 tgggcagcgg aggagtcgtg tcgtgcctga gagcgcagtc gagaaaccgg tgccaccatg
      601 gagacagaca cactcctgct atgggtactg ctgctctggg ttccaggttc cactggtgac
      661 acaagtttgt acaaaaaagt tggcaccacc ggtatggagc ccgccggcgc cggcggcgcc
      721 gccattctgc tggacgacga cgacgactac tacctcacaa cccccggcta ccccggctcc
      781 ggctctaacg cctccctgtc tcctccagcc gacctgttcc ccgccgcctc taaccactcg
      841 accgctgtcg tggtgtctgg cgacctggac ctgtacgtga tcattccact gggcatcttc
      901 ctcgccgtgc tgtctctcgt gacattcgcc ggcaacgtga tggtgctgca cgccctcaga
      961 acagagaaga agctccaaac agtgtctaac attttcatca tgtctctcgc cattgccgac
     1021 tgctctgtgg gcctgttcgc catgccactg agcgccgtgt acgccctgct gggctactgg
     1081 cctttcggcc tcgtggtgtg caacatgtgg ttgagtatgg actacgtggc cagcacagcc
     1141 tctattttca acctgttcgc cctgtccctc gaccggttct ggtctattat ttctccactc
     1201 agatacctca agaagcggac acgcaagcgg gccattatta tgtgctctag cgtgtggctc
     1261 gcctctctcc tgtggattat cccggttgta ggatggcacc agatgttcaa cgacggcagg
     1321 cgggacgtgc cagtggagga gtgcgacacc gagttcgccg acgagcaggt gttcaaggtg
     1381 ctgacagcca tcttcaactt ctacgtgcct atggccgcca tgattgccct gtacggcagg
     1441 attttccacg agattaagct cagagccgcg tctcagccag caccggccgg gccgcgcgac
     1501 accgacgcgc tggacctgga ggagggagga aacgtctata tcaaggccga caagcagaag
     1561 aacggcatca aggcgaactt cgtgatccgc cacaacatcg aggacggcgg cgtgcagctc
     1621 gcctaccact accagcagaa cacccccatc ggcgacggcc ccgtgctgct gcccgacaac
     1681 cactacctga gcgtgcagtc caaactttcg aaagacccca acgagaagcg cgatcacatg
     1741 gtcctgctgg agttcgtgac cgccgccggg atcactctcg gcatggacga gctgtacaag
     1801 ggcggtaccg gagggagcat ggtgagaaag ggcgaggagc tgttcaccgg ggtggtgccc
     1861 atcctggtcg agctggacgg cgacgtaaac ggccacaagt tcagcgtgtc cggcgagggt
     1921 gagggcgatg ccaccgaggg caagctgacc ctgaagttca tctgcaccac cggcaagctg
     1981 cccgtgccct ggcccaccct cgtgaccacc ctgacctacg gcgtgcagtg cttcagccgc
     2041 taccccgacc acatgaagca gcacgacttc ttcaagtccg ccatgcccga aggctacatc
     2101 caggagcgca ccatcttctt caaggacgac ggcaactaca agacccgcgc cgaggtgaag
     2161 ttcgagggcg acaccctggt gaaccgcatc gagctgaagg gcatcgactt caaggaggac
     2221 ggcaacatcc tggggcacaa gctggagtac aacaccggag cagcagcacg ctggcgcgcg
     2281 aagaaggccg ccttccagct gggcgtgatc ctgggcgcct tctgcctgtg cttcatgcct
     2341 tacttcatta gcttcatcat taccgccttc tgcaaggact gcgtgtctcc tgccgtgcac
     2401 tctattttca catggctggg ctacgtgaac tctacaacaa accctttcct gtacacatgg
     2461 acaaacgcca acttccggtc cgccttccgg aagatcctcc ggatgaagga gacaccaaag
     2521 aagccagtgt acatgcagat gcctcacaag acccctagac caaagaagca gccattagaa
     2581 gctagggtga gcggcacagc ctctggcggc accacagccc cagcccgcaa gtctgacgac
     2641 accagactct aagaattcga tatcaagctt atcgataatc aacctctgga ttacaaaatt
     2701 tgtgaaagat tgactggtat tcttaactat gttgctcctt ttacgctatg tggatacgct
     2761 gctttaatgc ctttgtatca tgctattgct tcccgtatgg ctttcatttt ctcctccttg
     2821 tataaatcct ggttgctgtc tctttatgag gagttgtggc ccgttgtcag gcaacgtggc
     2881 gtggtgtgca ctgtgtttgc tgacgcaacc cccactggtt ggggcattgc caccacctgt
     2941 cagctccttt ccgggacttt cgctttcccc ctccctattg ccacggcgga actcatcgcc
     3001 gcctgccttg cccgctgctg gacaggggct cggctgttgg gcactgacaa ttccgtggtg
     3061 ttgtcgggga aatcatcgtc ctttccttgg ctgctcgcct atgttgccac ctggattctg
     3121 cgcgggacgt ccttctgcta cgtcccttcg gccctcaatc cagcggacct tccttcccgc
     3181 ggcctgctgc cggctctgcg gcctcttccg cgtcttcgcc ttcgccctca gacgagtcgg
     3241 atctcccttt gggccgcctc cccgcatcga taccgagcgc tgctcgagag atctacgggt
     3301 ggcatccctg tgacccctcc ccagtgcctc tcctggccct ggaagttgcc actccagtgc
     3361 ccaccagcct tgtcctaata aaattaagtt gcatcatttt gtctgactag gtgtccttct
     3421 ataatattat ggggtggagg ggggtggtat ggagcaaggg gcaagttggg aagacaacct
     3481 gtagggcctg cggggtctat tgggaaccaa gctggagtgc agtggcacaa tcttggctca
     3541 ctgcaatctc cgcctcctgg gttcaagcga ttctcctgcc tcagcctccc gagttgttgg
     3601 gattccaggc atgcatgacc aggctcagct aatttttgtt tttttggtag agacggggtt
     3661 tcaccatatt ggccaggctg gtctccaact cctaatctca ggtgatctac ccaccttggc
     3721 ctcccaaatt gctgggatta caggcgtgaa ccactgctcc cttccctgtc cttctgattt
     3781 tgtaggtaac cacgtgcgga ccgagcggcc gcaggaaccc ctagtgatgg agttggccac
     3841 tccctctctg cgcgctcgct cgctcactga ggccgggcga ccaaaggtcg cccgacgccc
     3901 gggctttgcc cgggcggcct cagtgagcga gcgagcgcgc agctgcctgc aggggcgcct
     3961 gatgcggtat tttctcctta cgcatctgtg cggtatttca caccgcatac gtcaaagcaa
     4021 ccatagtacg cgccctgtag cggcgcatta agcgcggcgg gtgtggtggt tacgcgcagc
     4081 gtgaccgcta cacttgccag cgccctagcg cccgctcctt tcgctttctt cccttccttt
     4141 ctcgccacgt tcgccggctt tccccgtcaa gctctaaatc gggggctccc tttagggttc
     4201 cgatttagtg ctttacggca cctcgacccc aaaaaacttg atttgggtga tggttcacgt
     4261 agtgggccat cgccctgata gacggttttt cgccctttga cgttggagtc cacgttcttt
     4321 aatagtggac tcttgttcca aactggaaca acactcaacc ctatctcggg ctattctttt
     4381 gatttataag ggattttgcc gatttcggcc tattggttaa aaaatgagct gatttaacaa
     4441 aaatttaacg cgaattttaa caaaatatta acgtttacaa ttttatggtg cactctcagt
     4501 acaatctgct ctgatgccgc atagttaagc cagccccgac acccgccaac acccgctgac
     4561 gcgccctgac gggcttgtct gctcccggca tccgcttaca gacaagctgt gaccgtctcc
     4621 gggagctgca tgtgtcagag gttttcaccg tcatcaccga aacgcgcgag acgaaagggc
     4681 ctcgtgatac gcctattttt ataggttaat gtcatgataa taatggtttc ttagacgtca
     4741 ggtggcactt ttcggggaaa tgtgcgcgga acccctattt gtttattttt ctaaatacat
     4801 tcaaatatgt atccgctcat gagacaataa ccctgataaa tgcttcaata atattgaaaa
     4861 aggaagagta tgagtattca acatttccgt gtcgccctta ttcccttttt tgcggcattt
     4921 tgccttcctg tttttgctca cccagaaacg ctggtgaaag taaaagatgc tgaagatcag
     4981 ttgggtgcac gagtgggtta catcgaactg gatctcaaca gcggtaagat ccttgagagt
     5041 tttcgccccg aagaacgttt tccaatgatg agcactttta aagttctgct atgtggcgcg
     5101 gtattatccc gtattgacgc cgggcaagag caactcggtc gccgcataca ctattctcag
     5161 aatgacttgg ttgagtactc accagtcaca gaaaagcatc ttacggatgg catgacagta
     5221 agagaattat gcagtgctgc cataaccatg agtgataaca ctgcggccaa cttacttctg
     5281 acaacgatcg gaggaccgaa ggagctaacc gcttttttgc acaacatggg ggatcatgta
     5341 actcgccttg atcgttggga accggagctg aatgaagcca taccaaacga cgagcgtgac
     5401 accacgatgc ctgtagcaat ggcaacaacg ttgcgcaaac tattaactgg cgaactactt
     5461 actctagctt cccggcaaca attaatagac tggatggagg cggataaagt tgcaggacca
     5521 cttctgcgct cggcccttcc ggctggctgg tttattgctg ataaatctgg agccggtgag
     5581 cgtgggtctc gcggtatcat tgcagcactg gggccagatg gtaagccctc ccgtatcgta
     5641 gttatctaca cgacggggag tcaggcaact atggatgaac gaaatagaca gatcgctgag
     5701 ataggtgcct cactgattaa gcattggtaa ctgtcagacc aagtttactc atatatactt
     5761 tagattgatt taaaacttca tttttaattt aaaaggatct aggtgaagat cctttttgat
     5821 aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc agaccccgta
     5881 gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa
     5941 acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt
     6001 tttccgaagg taactggctt cagcagagcg cagataccaa atactgtcct tctagtgtag
     6061 ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta
     6121 atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca
     6181 agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag
     6241 cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga gctatgagaa
     6301 agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga
     6361 acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc
     6421 gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc
     6481 ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg ctggcctttt
     6541 gctcacatgt
//
