LOCUS       Exported                6403 bp ds-DNA     circular SYN 07-JUN-2022
DEFINITION  synthetic circular DNA
ACCESSION   VB220105-1309dnf
VERSION     .
KEYWORDS    VB220105-1309dnf.
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6403)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Monday, Aug 8, 2022 from SnapGene 5.0.8
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..6403
                     /organism="synthetic DNA construct"
     promoter        10..339
                     /label=SV40 promoter
                     /note="/__SeqFeature__=True"
                     /note="/__level__=0"
                     /note="/name=SV40 promoter"
                     /note="/official_designation=SV40 promoter"
                     /note="/uuid=57b0f08bed9c4d3b88821b51b0a9ac17"
                     /note="/vntifkey=30"
     primer_bind     607..626
                     /label=Minige-new_SD6_F
     misc_feature    1022..1027
                     /label=XhoI
                     /note="/__SeqFeature__=True"
                     /note="/__level__=0"
                     /note="/application_notes=Facilitates folding of shRNA into
                     hairpin."
                     /note="/name=XhoI"
                     /note="/official_designation=Loop"
                     /note="/uuid=a8ebc898e58a420897d3b88d510c8102"
                     /note="/vntifkey=21"
     misc_feature    1028..1411
                     /label=hRPH3A_c.444Gwt
                     /note="/__SeqFeature__=True"
                     /note="/__level__=0"
                     /note="/name=hRPH3A_c.444Gwt"
                     /note="/official_designation=hRPH3A_c.444Gwt"
                     /note="/uuid=18dab13e822c4d84a95fba1d9d9deb65"
                     /note="/vntifkey=21"
     misc_feature    1412..1417
                     /label=BamHI
                     /note="/__SeqFeature__=True"
                     /note="/__level__=0"
                     /note="/name=BamHI"
                     /note="/official_designation=BamHI"
                     /note="/uuid=2e24d26a052f4a8f9539513b7d1f974d"
                     /note="/vntifkey=21"
     misc_feature    2857..3090
                     /label=RRE
                     /note="/__SeqFeature__=True"
                     /note="/__level__=0"
                     /note="/application_notes=Rev protein binding site that 
                     allows
                     Rev-dependent nuclear export of viral RNA during viral 
                     packaging."
                     /note="/full_name=HIV-1 Rev response element"
                     /note="/name=RRE"
                     /note="/official_designation=RRE"
                     /note="/uuid=df07099e49674cf3aba3661b54e28bd7"
                     /note="/vntifkey=21"
     CDS             3275..3319
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="/__SeqFeature__=True"
                     /note="/__level__=0"
                     /note="/name=gp41 peptide"
                     /note="/official_designation=gp41 peptide"
                     /note="/uuid=80bcf8ce11ca408a8e96bb9de38e9e5e"
                     /note="/vntifkey=4"
                     /translation="KNEQELLELDKWASL"
     polyA_signal    3635..3769
                     /label=SV40 poly(A) signal
                     /note="/__SeqFeature__=True"
                     /note="/__level__=0"
                     /note="/name=SV40 poly(A) signal"
                     /note="/official_designation=SV40 poly(A) signal"
                     /note="/uuid=a1bc5125c7ac4bbbaee97ac15d7007c7"
                     /note="/vntifkey=25"
     promoter        4443..4547
                     /gene="bla"
                     /label=AmpR promoter
                     /note="/__SeqFeature__=True"
                     /note="/__level__=0"
                     /note="/name=AmpR promoter"
                     /note="/official_designation=AmpR promoter"
                     /note="/uuid=593ab506493a4ba19e374b1fa589278a"
                     /note="/vntifkey=30"
     CDS             4548..5408
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="/__SeqFeature__=True"
                     /note="/__level__=0"
                     /note="/application_notes=Allows E. coli to be resistant to
                     ampicillin."
                     /note="/full_name=Ampicillin resistance gene"
                     /note="/name=AmpR"
                     /note="/official_designation=Ampicillin"
                     /note="/uuid=6a7994dc58024d80b36e4a85ad2e1d99"
                     /note="/vntifkey=4"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      5579..6167
                     /direction=RIGHT
                     /label=ori
                     /note="/__SeqFeature__=True"
                     /note="/__level__=0"
                     /note="/name=ori"
                     /note="/official_designation=ori"
                     /note="/uuid=73c6cded97ba4795868bcd036b548ca2"
                     /note="/vntifkey=33"
ORIGIN
        1 ctgtggaatg tgtgtcagtt agggtgtgga aagtccccag gctccccagc aggcagaagt
       61 atgcaaagca tgcatctcaa ttagtcagca accaggtgtg gaaagtcccc aggctcccca
      121 gcaggcagaa gtatgcaaag catgcatctc aattagtcag caaccatagt cccgccccta
      181 actccgccca tcccgcccct aactccgccc agttccgccc attctccgcc ccatggctga
      241 ctaatttttt ttatttatgc agaggccgag gccgcctcgg cctctgagct attccagaag
      301 tagtgaggag gcttttttgg aggcctaggc ttttgcaaaa agcttggact gtgtttactt
      361 gcaatccccc aaaacagaca gaatggtgca tctgtccagt gaggagaagt ctgcggtcac
      421 tgccctgtgg ggcaaggtga atgtggaaga agttggtggt gaggccctgg gcaggctgct
      481 ggttgtctac ccatggaccc agaggttctt cgagtccttt ggggacctgt cctctgcaaa
      541 tgctgttatg aacaatccta aggtgaaggc tcatggcaag aaggtgctgg ctgccttcag
      601 tgagggtctg agtcacctgg acaacctcaa aggcaccttt gctaagctga gtgaactgca
      661 ctgtgacaag ctgcacgtgc tctagagtcg acccagcagt aagtaataca tgtaatgcaa
      721 cctatacaaa tagcaatagt agcattagta gtagcaataa taatagcaat agttgtgtgg
      781 tccatagtaa tcatagaata taggaaaata ttaagacaaa gaaaaataga caggttaatt
      841 gatagactaa tagaaagagc agaagacagt ggcaatgaga gtgaaggaga aatatcagca
      901 cttgtggaga tgggggtgga gatggggcac catgctcctt gggatgttga tgatctgtag
      961 tgctacagaa aaattgtggg tcacagtcta ttatggggta gggatcacca gaattctgga
     1021 gctcgagatt ctaactggcc ttgctcatag aaggaagtgg gagaaatgga agcaagttct
     1081 tcctctttac atccaccaag agaaagtggg gggctacgtt gccatgcctc ccatgagcat
     1141 ggctcatctg agtgtgttgg ctgtgtttca tccacagaac gtctgcacca agtgcggagt
     1201 ggagaccaac aaccgcctgc attctgtgtg gctctgcaaa atctgcattg agcagaggga
     1261 ggtgagtgcc ctggtcccac ctggtgccta gatcaccctc ctttcttggc cagcttaaga
     1321 ggtgccttaa gaggtttgta tgaaaggacc cagctcagca gtgaacattg acagaacaac
     1381 ctcagagtat ttggatgaat tatttctcct cggatcccag atatctggtg atcccgtacc
     1441 tgtgtggaag gaagcaacca ccactctatt ttgtgcatca gatgctaaag catatgatac
     1501 agaggtacat aatgtttggg ccacacatgc ctgtgtaccc acagacccca acccacaaga
     1561 agtagtattg gtaaatgtga cagaaaattt taacatgtgg aaaaatgaca tggtagaaca
     1621 gatgcatgag gatataatca gtttatggga tcaaagccta aagccatgtg taaaattaac
     1681 cccactctgt gttagtttaa agtgcactga tttgaagaat gatactaata ccaatagtag
     1741 tagcgggaga atgataatgg agaaaggaga gataaaaaac tgctctttca atatcagcac
     1801 aagcataaga ggtaaggtgc agaaagaata tgcatttttt tataaacttg atataatacc
     1861 aatagataat gatactacca gctatacgtt gacaagttgt aacacctcag tcattacaca
     1921 ggcctgtcca aaggtatcct ttgagccaat tcccatacat tattgtgccc cggctggttt
     1981 tgcgattcta aaatgtaata ataagacgtt caatggaaca ggaccatgta caaatgtcag
     2041 cacagtacaa tgtacacatg gaattaggcc agtagtatca actcaactgc tgttaaatgg
     2101 cagtctagca gaagaagagg tagtaattag atctgtcaat ttcacggaca atgctaaaac
     2161 cataatagta cagctgaaca catctgtaga aattaattgt acaagaccca acaacaatac
     2221 aagaaaaaaa atccgtatcc agaggggacc agggagagca tttgttacaa taggaaaaat
     2281 aggaaatatg agacaagcac attgtaacat tagtagagca aaatggaatg ccactttaaa
     2341 acagatagct agcaaattaa gagaacaatt tggaaataat aaaacaataa tctttaagca
     2401 atcctcagga ggggacccag aaattgtaac gcacagtttt aattgtggag gggaattttt
     2461 ctactgtaat tcaacacaac tgtttaatag tacttggttt aatagtactt ggagtactga
     2521 agggtcaaat aacactgaag gaagtgacac aatcacactc ccatgcagaa taaaacaatt
     2581 tataaacatg tggcaggaag taggaaaagc aatgtatgcc cctcccatca gcggacaaat
     2641 tagatgttca tcaaatatta cagggctgct attaacaaga gatggtggta ataacaacaa
     2701 tgggtccgag atcttcagac ctggaggagg agatatgagg gacaattgga gaagtgaatt
     2761 atataaatat aaagtagtaa aaattgaacc attaggagta gcacccacca aggcaaagag
     2821 aagagtggtg cagagagaaa aaagagcagt gggaatagga gctttgttcc ttgggttctt
     2881 gggagcagca ggaagcacta tgggcgcagc gtcaatgacg ctgacggtac aggccagaca
     2941 attattgtct ggtatagtgc agcagcagaa caatttgctg agggctattg aggcgcaaca
     3001 gcatctgttg caactcacag tctggggcat caagcagctc caggcaagaa tcctggctgt
     3061 ggaaagatac ctaaaggatc aacagctcct ggggatttgg ggttgctctg gaaaactact
     3121 ttgcaccact gctgtgcctt ggaatgctag ttggagtaat aaatctctgg aacagatttg
     3181 gaatcacacg acgtggatgg agtgggacag agaaattaac aattacacaa gcttaataca
     3241 ctccttaatt gaagaatcgc aaaaccagca agaaaagaat gaacaagaat tattggaatt
     3301 agataaatgg gcaagtttgt ggaattggtt taacataaca aattggctgt ggtatataaa
     3361 attattcata atgatagtag gaggcttggt aggtttaaga atagtttttg ctgtactttc
     3421 tgtagtgaat agagttaggc agggatattc accattatcg tttcagacct ggagatctcc
     3481 cgaggggacc cgacaggccc gaaggaatag aagaagaagg tggagagaga gacagagaca
     3541 gatccatttc gaccaattca ctcctcaggt gcaggctgcc tatcagaagg tggtggctgg
     3601 tgtggccaat gccctggctc acaaatacca ctgagatcca gacatgataa gatacattga
     3661 tgagtttgga caaaccacaa ctagaatgca gtgaaaaaaa tgctttattt gtgaaatttg
     3721 tgatgctatt gctttatttg taaccattat aagctgcaat aaacaagtta acaacaacaa
     3781 ttgcattcat tttatgtttc aggttcaggg ggaggtgtgg gaggtttttt aaagcaagta
     3841 aaacctctac aaatgtggta tggctgatta tgatccccag gaagctcctc tgtgtcctca
     3901 taaaccctaa cctcctctac ttgagaggac attccaatca taggctgccc atccaccctc
     3961 tgtgtcctcc tgttaattag gtcacttaac aaaaaggaaa ttgggtaggg gtttttcaca
     4021 gaccgctttc taagggtaat tttaaaatat ctgggaagtc ccttccactg ctgtgttcca
     4081 gaagtgttgg taaacagccc acaaatgtca acagcagaaa catacaagct gtcagctttg
     4141 cacaagggcc caacaccctg ctcatcaaga agcactgtgg ttgctgtgtt agtaatgtgc
     4201 aaaacaggag gcacattttc cccacctgtg taggttccaa aatatctagt gttttcattt
     4261 ttacttggat caggaaccca gcactccact ggataagcat tatccttatc caaaacagcc
     4321 ttgtggtcag tgttcatctg ctgactgtca actgtagcat tttttggggt tacagtttga
     4381 gcaggatatt tggtcctgta gtttgctaac acaccccagg tggcactttt cggggaaatg
     4441 tgcgcggaac ccctatttgt ttatttttct aaatacattc aaatatgtat ccgctcatga
     4501 gacaataacc ctgataaatg cttcaataat attgaaaaag gaagagtatg agtattcaac
     4561 atttccgtgt cgcccttatt cccttttttg cggcattttg ccttcctgtt tttgctcacc
     4621 cagaaacgct ggtgaaagta aaagatgctg aagatcagtt gggtgcacga gtgggttaca
     4681 tcgaactgga tctcaacagc ggtaagatcc ttgagagttt tcgccccgaa gaacgttttc
     4741 caatgatgag cacttttaaa gttctgctat gtggcgcggt attatcccgt attgacgccg
     4801 ggcaagagca actcggtcgc cgcatacact attctcagaa tgacttggtt gagtactcac
     4861 cagtcacaga aaagcatctt acggatggca tgacagtaag agaattatgc agtgctgcca
     4921 taaccatgag tgataacact gcggccaact tacttctgac aacgatcgga ggaccgaagg
     4981 agctaaccgc ttttttgcac aacatggggg atcatgtaac tcgccttgat cgttgggaac
     5041 cggagctgaa tgaagccata ccaaacgacg agcgtgacac cacgatgcct gtagcaatgg
     5101 caacaacgtt gcgcaaacta ttaactggcg aactacttac tctagcttcc cggcaacaat
     5161 taatagactg gatggaggcg gataaagttg caggaccact tctgcgctcg gcccttccgg
     5221 ctggctggtt tattgctgat aaatctggag ccggtgagcg tgggtctcgc ggtatcattg
     5281 cagcactggg gccagatggt aagccctccc gtatcgtagt tatctacacg acggggagtc
     5341 aggcaactat ggatgaacga aatagacaga tcgctgagat aggtgcctca ctgattaagc
     5401 attggtaact gtcagaccaa gtttactcat atatacttta gattgattta aaacttcatt
     5461 tttaatttaa aaggatctag gtgaagatcc tttttgataa tctcatgacc aaaatcctta
     5521 acggtgagtt ttcgttccac tgagcgtcag accccgtaga aaagatcaaa ggatcttctt
     5581 gagatccttt ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag
     5641 cggtggtttg tttgccggat caagagctac caactctttt tccgaaggta actggcttca
     5701 gcagagcgca gataccaaat actgtccttc tagtgtagcc gtagttaggc caccacttca
     5761 agaactctgt agcaccgcct acatacctcg ctctgctaat cctgttacca gtggctgctg
     5821 ccagtggcga taagtcgtgt cttaccgggt tggactcaag acgatagtta ccggataagg
     5881 cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag cgaacgacct
     5941 acaccgaact gagataccta cagcgcgagc attgagaaag cgccacgctt cccgaaggga
     6001 gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc acgagggagc
     6061 ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac ctctgacttg
     6121 agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac gccagcaacg
     6181 cggccttttt acggttcctg gccttttgct ggccttttgc tcacatgttc tttcctgcgt
     6241 tatcccctga ttctgtggat aaccgtatta ccgcctttga gtgagctgat accgctcgcc
     6301 gcagccgaac gaccgagcgc agcgagtcag tgagcgagga agcggaagag cgcccaatac
     6361 gcaaaccgcc tctccccgcg cgttggccga ttcattaatg cag
//
