LOCUS       Exported                6401 bp ds-DNA     circular SYN 07-JUN-2022
DEFINITION  synthetic circular DNA
ACCESSION   VB220105-1313dtw
VERSION     .
KEYWORDS    VB220105-1313dtw.
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6401)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Monday, Aug 8, 2022 from SnapGene 5.0.8
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..6401
                     /organism="synthetic DNA construct"
     promoter        10..339
                     /label=SV40 promoter
                     /note="/__SeqFeature__=True"
                     /note="/__level__=0"
                     /note="/name=SV40 promoter"
                     /note="/official_designation=SV40 promoter"
                     /note="/uuid=57b0f08bed9c4d3b88821b51b0a9ac17"
                     /note="/vntifkey=30"
     misc_feature    1022..1027
                     /label=XhoI
                     /note="/__SeqFeature__=True"
                     /note="/__level__=0"
                     /note="/application_notes=Facilitates folding of shRNA into
                     hairpin."
                     /note="/name=XhoI"
                     /note="/official_designation=Loop"
                     /note="/uuid=a8ebc898e58a420897d3b88d510c8102"
                     /note="/vntifkey=21"
     misc_feature    1028..1409
                     /label=hRPH3A_c.1853Awt
                     /note="/__SeqFeature__=True"
                     /note="/__level__=0"
                     /note="/name=hRPH3A_c.1853Awt"
                     /note="/official_designation=hRPH3A_c.1853Awt"
                     /note="/uuid=db707443973a4e58863d02ee686b52da"
                     /note="/vntifkey=21"
     misc_feature    1410..1415
                     /label=BamHI
                     /note="/__SeqFeature__=True"
                     /note="/__level__=0"
                     /note="/name=BamHI"
                     /note="/official_designation=BamHI"
                     /note="/uuid=2e24d26a052f4a8f9539513b7d1f974d"
                     /note="/vntifkey=21"
     misc_feature    2855..3088
                     /label=RRE
                     /note="/__SeqFeature__=True"
                     /note="/__level__=0"
                     /note="/application_notes=Rev protein binding site that 
                     allows
                     Rev-dependent nuclear export of viral RNA during viral 
                     packaging."
                     /note="/full_name=HIV-1 Rev response element"
                     /note="/name=RRE"
                     /note="/official_designation=RRE"
                     /note="/uuid=df07099e49674cf3aba3661b54e28bd7"
                     /note="/vntifkey=21"
     CDS             3273..3317
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="/__SeqFeature__=True"
                     /note="/__level__=0"
                     /note="/name=gp41 peptide"
                     /note="/official_designation=gp41 peptide"
                     /note="/uuid=80bcf8ce11ca408a8e96bb9de38e9e5e"
                     /note="/vntifkey=4"
                     /translation="KNEQELLELDKWASL"
     polyA_signal    3633..3767
                     /label=SV40 poly(A) signal
                     /note="/__SeqFeature__=True"
                     /note="/__level__=0"
                     /note="/name=SV40 poly(A) signal"
                     /note="/official_designation=SV40 poly(A) signal"
                     /note="/uuid=a1bc5125c7ac4bbbaee97ac15d7007c7"
                     /note="/vntifkey=25"
     promoter        4441..4545
                     /gene="bla"
                     /label=AmpR promoter
                     /note="/__SeqFeature__=True"
                     /note="/__level__=0"
                     /note="/name=AmpR promoter"
                     /note="/official_designation=AmpR promoter"
                     /note="/uuid=593ab506493a4ba19e374b1fa589278a"
                     /note="/vntifkey=30"
     CDS             4546..5406
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="/__SeqFeature__=True"
                     /note="/__level__=0"
                     /note="/application_notes=Allows E. coli to be resistant to
                     ampicillin."
                     /note="/full_name=Ampicillin resistance gene"
                     /note="/name=AmpR"
                     /note="/official_designation=Ampicillin"
                     /note="/uuid=6a7994dc58024d80b36e4a85ad2e1d99"
                     /note="/vntifkey=4"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      5577..6165
                     /direction=RIGHT
                     /label=ori
                     /note="/__SeqFeature__=True"
                     /note="/__level__=0"
                     /note="/name=ori"
                     /note="/official_designation=ori"
                     /note="/uuid=73c6cded97ba4795868bcd036b548ca2"
                     /note="/vntifkey=33"
ORIGIN
        1 ctgtggaatg tgtgtcagtt agggtgtgga aagtccccag gctccccagc aggcagaagt
       61 atgcaaagca tgcatctcaa ttagtcagca accaggtgtg gaaagtcccc aggctcccca
      121 gcaggcagaa gtatgcaaag catgcatctc aattagtcag caaccatagt cccgccccta
      181 actccgccca tcccgcccct aactccgccc agttccgccc attctccgcc ccatggctga
      241 ctaatttttt ttatttatgc agaggccgag gccgcctcgg cctctgagct attccagaag
      301 tagtgaggag gcttttttgg aggcctaggc ttttgcaaaa agcttggact gtgtttactt
      361 gcaatccccc aaaacagaca gaatggtgca tctgtccagt gaggagaagt ctgcggtcac
      421 tgccctgtgg ggcaaggtga atgtggaaga agttggtggt gaggccctgg gcaggctgct
      481 ggttgtctac ccatggaccc agaggttctt cgagtccttt ggggacctgt cctctgcaaa
      541 tgctgttatg aacaatccta aggtgaaggc tcatggcaag aaggtgctgg ctgccttcag
      601 tgagggtctg agtcacctgg acaacctcaa aggcaccttt gctaagctga gtgaactgca
      661 ctgtgacaag ctgcacgtgc tctagagtcg acccagcagt aagtaataca tgtaatgcaa
      721 cctatacaaa tagcaatagt agcattagta gtagcaataa taatagcaat agttgtgtgg
      781 tccatagtaa tcatagaata taggaaaata ttaagacaaa gaaaaataga caggttaatt
      841 gatagactaa tagaaagagc agaagacagt ggcaatgaga gtgaaggaga aatatcagca
      901 cttgtggaga tgggggtgga gatggggcac catgctcctt gggatgttga tgatctgtag
      961 tgctacagaa aaattgtggg tcacagtcta ttatggggta gggatcacca gaattctgga
     1021 gctcgaggat gctctaatgc atattggtca ttatcaatgt tgatcccttt ctgatttatt
     1081 catcaattca ccctgataaa gaaggggcct ttggggtcaa gaggctgttc ttattcatta
     1141 aacgtcatcc ataatagcat gttgtgtcgc ctcccagctg gctgaaaccg gacatgggaa
     1201 agaaggccaa acacaagact caaattaaaa agaaaacctt gaatcccgaa ttcaatgagg
     1261 taaggctgcc ctattctttt tcatgctctg ggatatttgc tgtgtgttag agaggcacaa
     1321 atagccacat agccattaaa tatgtggcca catagccatt aaatgcagcc atttgaaatg
     1381 atgatgtaga aaaatatttg aaaaatattg gatcccagat atctggtgat cccgtacctg
     1441 tgtggaagga agcaaccacc actctatttt gtgcatcaga tgctaaagca tatgatacag
     1501 aggtacataa tgtttgggcc acacatgcct gtgtacccac agaccccaac ccacaagaag
     1561 tagtattggt aaatgtgaca gaaaatttta acatgtggaa aaatgacatg gtagaacaga
     1621 tgcatgagga tataatcagt ttatgggatc aaagcctaaa gccatgtgta aaattaaccc
     1681 cactctgtgt tagtttaaag tgcactgatt tgaagaatga tactaatacc aatagtagta
     1741 gcgggagaat gataatggag aaaggagaga taaaaaactg ctctttcaat atcagcacaa
     1801 gcataagagg taaggtgcag aaagaatatg cattttttta taaacttgat ataataccaa
     1861 tagataatga tactaccagc tatacgttga caagttgtaa cacctcagtc attacacagg
     1921 cctgtccaaa ggtatccttt gagccaattc ccatacatta ttgtgccccg gctggttttg
     1981 cgattctaaa atgtaataat aagacgttca atggaacagg accatgtaca aatgtcagca
     2041 cagtacaatg tacacatgga attaggccag tagtatcaac tcaactgctg ttaaatggca
     2101 gtctagcaga agaagaggta gtaattagat ctgtcaattt cacggacaat gctaaaacca
     2161 taatagtaca gctgaacaca tctgtagaaa ttaattgtac aagacccaac aacaatacaa
     2221 gaaaaaaaat ccgtatccag aggggaccag ggagagcatt tgttacaata ggaaaaatag
     2281 gaaatatgag acaagcacat tgtaacatta gtagagcaaa atggaatgcc actttaaaac
     2341 agatagctag caaattaaga gaacaatttg gaaataataa aacaataatc tttaagcaat
     2401 cctcaggagg ggacccagaa attgtaacgc acagttttaa ttgtggaggg gaatttttct
     2461 actgtaattc aacacaactg tttaatagta cttggtttaa tagtacttgg agtactgaag
     2521 ggtcaaataa cactgaagga agtgacacaa tcacactccc atgcagaata aaacaattta
     2581 taaacatgtg gcaggaagta ggaaaagcaa tgtatgcccc tcccatcagc ggacaaatta
     2641 gatgttcatc aaatattaca gggctgctat taacaagaga tggtggtaat aacaacaatg
     2701 ggtccgagat cttcagacct ggaggaggag atatgaggga caattggaga agtgaattat
     2761 ataaatataa agtagtaaaa attgaaccat taggagtagc acccaccaag gcaaagagaa
     2821 gagtggtgca gagagaaaaa agagcagtgg gaataggagc tttgttcctt gggttcttgg
     2881 gagcagcagg aagcactatg ggcgcagcgt caatgacgct gacggtacag gccagacaat
     2941 tattgtctgg tatagtgcag cagcagaaca atttgctgag ggctattgag gcgcaacagc
     3001 atctgttgca actcacagtc tggggcatca agcagctcca ggcaagaatc ctggctgtgg
     3061 aaagatacct aaaggatcaa cagctcctgg ggatttgggg ttgctctgga aaactacttt
     3121 gcaccactgc tgtgccttgg aatgctagtt ggagtaataa atctctggaa cagatttgga
     3181 atcacacgac gtggatggag tgggacagag aaattaacaa ttacacaagc ttaatacact
     3241 ccttaattga agaatcgcaa aaccagcaag aaaagaatga acaagaatta ttggaattag
     3301 ataaatgggc aagtttgtgg aattggttta acataacaaa ttggctgtgg tatataaaat
     3361 tattcataat gatagtagga ggcttggtag gtttaagaat agtttttgct gtactttctg
     3421 tagtgaatag agttaggcag ggatattcac cattatcgtt tcagacctgg agatctcccg
     3481 aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga cagagacaga
     3541 tccatttcga ccaattcact cctcaggtgc aggctgccta tcagaaggtg gtggctggtg
     3601 tggccaatgc cctggctcac aaataccact gagatccaga catgataaga tacattgatg
     3661 agtttggaca aaccacaact agaatgcagt gaaaaaaatg ctttatttgt gaaatttgtg
     3721 atgctattgc tttatttgta accattataa gctgcaataa acaagttaac aacaacaatt
     3781 gcattcattt tatgtttcag gttcaggggg aggtgtggga ggttttttaa agcaagtaaa
     3841 acctctacaa atgtggtatg gctgattatg atccccagga agctcctctg tgtcctcata
     3901 aaccctaacc tcctctactt gagaggacat tccaatcata ggctgcccat ccaccctctg
     3961 tgtcctcctg ttaattaggt cacttaacaa aaaggaaatt gggtaggggt ttttcacaga
     4021 ccgctttcta agggtaattt taaaatatct gggaagtccc ttccactgct gtgttccaga
     4081 agtgttggta aacagcccac aaatgtcaac agcagaaaca tacaagctgt cagctttgca
     4141 caagggccca acaccctgct catcaagaag cactgtggtt gctgtgttag taatgtgcaa
     4201 aacaggaggc acattttccc cacctgtgta ggttccaaaa tatctagtgt tttcattttt
     4261 acttggatca ggaacccagc actccactgg ataagcatta tccttatcca aaacagcctt
     4321 gtggtcagtg ttcatctgct gactgtcaac tgtagcattt tttggggtta cagtttgagc
     4381 aggatatttg gtcctgtagt ttgctaacac accccaggtg gcacttttcg gggaaatgtg
     4441 cgcggaaccc ctatttgttt atttttctaa atacattcaa atatgtatcc gctcatgaga
     4501 caataaccct gataaatgct tcaataatat tgaaaaagga agagtatgag tattcaacat
     4561 ttccgtgtcg cccttattcc cttttttgcg gcattttgcc ttcctgtttt tgctcaccca
     4621 gaaacgctgg tgaaagtaaa agatgctgaa gatcagttgg gtgcacgagt gggttacatc
     4681 gaactggatc tcaacagcgg taagatcctt gagagttttc gccccgaaga acgttttcca
     4741 atgatgagca cttttaaagt tctgctatgt ggcgcggtat tatcccgtat tgacgccggg
     4801 caagagcaac tcggtcgccg catacactat tctcagaatg acttggttga gtactcacca
     4861 gtcacagaaa agcatcttac ggatggcatg acagtaagag aattatgcag tgctgccata
     4921 accatgagtg ataacactgc ggccaactta cttctgacaa cgatcggagg accgaaggag
     4981 ctaaccgctt ttttgcacaa catgggggat catgtaactc gccttgatcg ttgggaaccg
     5041 gagctgaatg aagccatacc aaacgacgag cgtgacacca cgatgcctgt agcaatggca
     5101 acaacgttgc gcaaactatt aactggcgaa ctacttactc tagcttcccg gcaacaatta
     5161 atagactgga tggaggcgga taaagttgca ggaccacttc tgcgctcggc ccttccggct
     5221 ggctggttta ttgctgataa atctggagcc ggtgagcgtg ggtctcgcgg tatcattgca
     5281 gcactggggc cagatggtaa gccctcccgt atcgtagtta tctacacgac ggggagtcag
     5341 gcaactatgg atgaacgaaa tagacagatc gctgagatag gtgcctcact gattaagcat
     5401 tggtaactgt cagaccaagt ttactcatat atactttaga ttgatttaaa acttcatttt
     5461 taatttaaaa ggatctaggt gaagatcctt tttgataatc tcatgaccaa aatccttaac
     5521 ggtgagtttt cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga
     5581 gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg
     5641 gtggtttgtt tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc
     5701 agagcgcaga taccaaatac tgtccttcta gtgtagccgt agttaggcca ccacttcaag
     5761 aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc
     5821 agtggcgata agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg
     5881 cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac
     5941 accgaactga gatacctaca gcgcgagcat tgagaaagcg ccacgcttcc cgaagggaga
     6001 aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt
     6061 ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag
     6121 cgtcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg
     6181 gcctttttac ggttcctggc cttttgctgg ccttttgctc acatgttctt tcctgcgtta
     6241 tcccctgatt ctgtggataa ccgtattacc gcctttgagt gagctgatac cgctcgccgc
     6301 agccgaacga ccgagcgcag cgagtcagtg agcgaggaag cggaagagcg cccaatacgc
     6361 aaaccgcctc tccccgcgcg ttggccgatt cattaatgca g
//
