LOCUS       pThpR                   6470 bp ds-DNA     circular     14-OCT-2022
DEFINITION  .
FEATURES             Location/Qualifiers
     misc_feature    complement(212..273)
                     /label="L3S2P00 terminator"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
     misc_feature    complement(542..1357)
                     /label="KanR"
                     /ApEinfo_revcolor="#75c6a9"
                     /ApEinfo_fwdcolor="#75c6a9"
     misc_feature    1719..2558
                     /label="p15A"
                     /ApEinfo_revcolor="#faac61"
                     /ApEinfo_fwdcolor="#faac61"
     misc_feature    complement(2756..3715)
                     /label="LacI"
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_fwdcolor="#85dae9"
     misc_feature    4011..4093
                     /label="rpoC terminator"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
     Promoter        4195..4240
                     /label="Pramr promoter"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     Promoter        4250..4330
                     /label="PlmJ"
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_fwdcolor="#ff9ccd"
     misc_feature    4361..5086
                     /label="sfGFP"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_fwdcolor="#b1ff67"
     misc_feature    5114..5488
                     /label="Sh ble"
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
     Terminator      5558..5639
                     /label="rnpB T1 terminator"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
     Terminator      complement(5721..5777)
                     /label="L3S2P22 terminator"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
     misc_feature    complement(5849..6433)
                     /label="THP4.1"
                     /ApEinfo_revcolor="#c7b0e3"
                     /ApEinfo_fwdcolor="#c7b0e3"
     Promoter        complement(6458..62)
                     /label="RiboJ"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
ORIGIN
        1 TTTGTAGAGG CTGTTTCGTC CTCACGGACT CATCAGACCG GAAAGCACAT CCGGTGACAG
       61 CTGTGCTCAG TATCTTGATT GCTAGCACTA TACCTAGGAC TGAGCTAGCC GTAAAGGTAC
      121 TTTTCAAAGG TCGACAGCCC ATGCCCTTGC GTTCGGCAGG TGTACAATGA TACGAGGTAA
      181 TGAAGATGAA GTCCATACAA TCGATAGATT GGGACCAAAA CGAAAAAAGG GGAGCGGTTT
      241 CCCGCTCCCC TCTTTCTGGA ATTTGGTACC GAGTCGCACC TGATTGCCCG ACATTATCGC
      301 ACGGTGTCTC ATCTCTGATA ACGCATATTG TCGTTAGAAC TCGGCGCGGC CGCTCACACT
      361 GCTTCCGGTA GTCAATAAAC CGGTAAACCA GCAATAGACA TAAGCGGCTA TTTAACGACC
      421 CTGCCCTGAA CCGACGACCG GGTCGAATTT GCTTTCGAAT TTCTGCCATT CATCCGCTTA
      481 TTATCACTTA TTCAGGCGTA GCAACCAGGC GTTTAAGGGC ACCAATAACT GCCTTAAAAA
      541 ATTAGAAAAA CTCATCGAGC ATCAAATGAA ACTGCAATTT ATTCATATCA GGATTATCAA
      601 TACCATATTT TTGAAAAAGC CGTTTCTGTA ATGAAGGAGA AAACTCACCG AGGCAGTTCC
      661 ATAGGATGGC AAGATCCTGG TATCGGTCTG CGATTCCGAC TCGTCCAACA TCAATACAAC
      721 CTATTAATTT CCCCTCGTCA AAAATAAGGT TATCAAGTGA GAAATCACCA TGAGTGACGA
      781 CTGAATCCGG TGAGAATGGC AAAAGTTTAT GCATTTCTTT CCAGACTTGT TCAACAGGCC
      841 AGCCATTACG CTCGTCATCA AAATCACTCG CATCAACCAA ACCGTTATTC ATTCGTGATT
      901 GCGCCTGAGC GAGACGAAAT ACGCGGTCGC TGTTAAAAGG ACAATTACAA ACAGGAATCG
      961 AATGCAACCG GCGCAGGAAC ACTGCCAGCG CATCAACAAT ATTTTCACCT GAATCAGGAT
     1021 ATTCTTCTAA TACCTGGAAT GCTGTTTTCC CGGGGATCGC AGTGGTGAGT AACCATGCAT
     1081 CATCAGGAGT ACGGATAAAA TGCTTGATGG TCGGAAGAGG CATAAATTCC GTCAGCCAGT
     1141 TTAGTCTGAC CATCTCATCT GTAACATCAT TGGCAACGCT ACCTTTGCCA TGTTTCAGAA
     1201 ACAACTCTGG CGCATCGGGC TTCCCATACA ATCGATAGAT TGTCGCACCT GATTGCCCGA
     1261 CATTATCGCG AGCCCATTTA TACCCATATA AATCAGCATC CATGTTGGAA TTTAATCGCG
     1321 GCCTAGAGCA AGACGTTTCC CGTTGAATAT GGCTCATTTT AGCTTCCTTA GCTCCTGAAA
     1381 ATCTCGATAA CTCAAAAAAT ACGCCCGGTA GTGATCTTAT TTCATTATGG TGAAAGTTGG
     1441 AACCTCTTAC GTGCCGATCA ACGTCTCATT TTCGCCAAAA GTTGGCCCAG GGCTTCCCGG
     1501 TATCAACAGG GACACCAGGA TTTATTTATT CTGCGAAGTG ATCTTCCGTC ACAGGTATTT
     1561 ATTCGGCGCA AAGTGCGTCG GGTGATGCTG CCAACTTACT GATTTAGTGT ATGATGGTGT
     1621 TTTTGAGGTG CTCCAGTGGC TTCTGTTTCT ATCAGCTGTC CCTCCTGTTC AGCTACTGAC
     1681 GGGGTGGTGC GTAACGGCAA AAGCACCGCC GGACATCAGC GCTAGCGGAG TGTATACTGG
     1741 CTTACTATGT TGGCACTGAT GAGGGTGTCA GTGAAGTGCT TCATGTGGCA GGAGAAAAAA
     1801 GGCTGCACCG GTGCGTCAGC AGAATATGTG ATACAGGATA TATTCCGCTT CCTCGCTCAC
     1861 TGACTCGCTA CGCTCGGTCG TTCGACTGCG GCGAGCGGAA ATGGCTTACG AACGGGGCGG
     1921 AGATTTCCTG GAAGATGCCA GGAAGATACT TAACAGGGAA GTGAGATGGC CGCGGCAAAG
     1981 CCGTTTTTCC ATAGGCTCCG CCCCCCTGAC AAGCATCACG AAATCTGACG CTCAAATCAG
     2041 TGGTGGCGAA ACCCGACAGG ACTATAAAGA TACCAGGCGT TTCCCCCTGG CGGCTCCCTC
     2101 GTGCGCTCTC CTGTTCCTGC CTTTCGGTTT ACCGGTGTCA TTCCGCTGTT ATGGCCGCGT
     2161 TTGTCTCATT CCACGCCTGA CACTCAGTTC CGGGCAGGCA GTTCGCTCCA AGCTGGACTG
     2221 TATGCACGAA CCCCCCGTTC AGTCCGACCG CTGCGCCTTA TCCGGTAACT ATCGTCTTGA
     2281 GTCCAACCCG GAAAGACATG CAAAAGCACC ACTGGCAGCA GCCACTGGTA ATTGATTTAG
     2341 AGGAGTTAGT CTTGAAGTCA TGCGCCGGTT AAGGCTAAAC TGAAAGGACA AGTTTTGGTG
     2401 ACTGCGCTCC TCCAAGCCAG TTACCTCGGT TCAAAGAGTT GGTAGCTCAG AGAACCTTCG
     2461 AAAAACCGCC CTGCAAGGCG GTTTTTTCGT TTTCAGAGCA AGAGATTACG CGCAGACCAA
     2521 AACGATCTCA AGAAGATCAT CTTATTAATC AGATAAAATA TTTCTAGATT TCAGTGCAAT
     2581 TTATCTCTTC AAATGTAGCA CCTGAAGTCA GCCCCATACG ATATAAGTTG TAATTCTCAT
     2641 GTTAGTCATG CCCCGCGCCC ACCGGAAGGA GCTGACTGGG TTGAAGGCTC TCAAGGGCAT
     2701 CGGTCGAGAT CCCGGTGCCT AATGAGTGAG CTAACTTACA TTAATTGCGT TGCGCTCACT
     2761 GCCCGCTTTC CAGTCGGGAA ACCTGTCGTG CCAGCTGCAT TAATGAATCG GCCAACGCGC
     2821 GGGGAGAGGC GGTTTGCGTA TTGGGCGCCA GGGTGGTTTT TCTTTTCACC AGTGAGACGG
     2881 GCAACAGCTG ATTGCCCTTC ACCGCCTGGC CCTGAGAGAG TTGCAGCAAG CGGTCCACGC
     2941 TGGTTTGCCC CAGCAGGCGA AAATCCTGTT TGATGGTGGT TAACGGCGGG ATATAACATG
     3001 AGCTATCTTC GGTATCGTCG TATCCCACTA CCGAGATATC CGCACCAACG CGCAGCCCGG
     3061 ACTCGGTAAT GGCGCGCATT GCGCCCAGCG CCATCTGATC GTTGGCAACC AGCATCGCAG
     3121 TGGGAACGAT GCCCTCATTC AGCATTTGCA TGGTTTGTTG AAAACCGGAC ATGGCACTCC
     3181 AGTCGCCTTC CCGTTCCGCT ATCGGCTGAA TTTGATTGCG AGTGAGATAT TTATGCCAGC
     3241 CAGCCAGACG CAGACGCGCC GAGACAGAAC TTAATGGGCC CGCTAACAGC GCGATTTGCT
     3301 GGTGACCCAA TGCGACCAGA TGCTCCACGC CCAGTCGCGT ACCATCTTCA TGGGAGAAAA
     3361 TAATACTGTT GATGGGTGTC TGGTCAGAGA CATCAAGAAA TAACGCCGGA ACATTAGTGC
     3421 AGGCAGCTTC CACAGCAATG GCATCCTGGT CATCCAGCGG ATAGTTAATG ATCAGCCCAC
     3481 TGACGCGTTG CGCGAGAAGA TTGTGCACCG CCGCTTTACA GGCTTCGACG CCGCTTCGTT
     3541 CTACCATCGA CACCACCACG CTGGCACCCA GTTGATCGGC GCGAGATTTA ATCGCCGCGA
     3601 CAATTTGCGA CGGCGCGTGC AGGGCCAGAC TAGAGGTGGC AACGCCAATC AGCAACGACT
     3661 GTTTGCCCGC CAGTTGTTGT GCCACGCGGT TGGGAATGTA ATTCAGCTCC GCCATCGCCG
     3721 CTTCCACTTT TTCCCGCGTT TTCGCAGAAA CGTGGCTGGC CTGGTTCACC ACGCGGGAAA
     3781 CGGTCTGATA AGAGACACCG GCATACTCTG CGACATCGTA TAACGTTACT GGTTTCACAT
     3841 TCACCACCCT GAATTGACTC TCTTCCGGGC GCTATCATGC CATACCGCGA AAGGTTTTGC
     3901 GCCATTCGAT GGTGTCCGGG ATCTCGACGC TCTCCCTTAT GCGACGCGGC CGCGGCATCA
     3961 GAGCAGATTG TACTGTGTCC TCAATGGTTC GTTGTGATGG CGGTAGGAAT GTAATCGTTA
     4021 ATCCGCAAAT AACGTAAAAA CCCGCTTCGG CGGGTTTTTT TATGGGGGGA GTTTAGGGAA
     4081 AGAGCATTTG TCATCCCGTT GAATATGGCT CGCATCTTAT CGAGCATACT ATCACGTCGG
     4141 CGACCACTAG TCAGTTAACG CAAGGGCATG GGCTGTCGAC CTTTGAAAAG TACCTTGACG
     4201 GCGTATCTTT GCTTTCTATA ATGAGTGCTT ACTCACTCAT ACAATAGTCA GTCATAAGTC
     4261 TGGGCTAAGC CCACTGATGA GTCGCTGAAA TGCGACGAAA CTTATGACCT CTACAAATAA
     4321 TTTTGTTTAA CGTAAACCTC CGGGTTAATA AGGAGTAATT ATGGCATCCA AGGGCGAGGA
     4381 GCTCTTTACT GGCGTAGTAC CAATTCTCGT AGAGCTCGAT GGCGATGTAA ATGGCCATAA
     4441 GTTTTCCGTA CGCGGCGAGG GCGAGGGCGA TGCAACTAAC GGCAAGCTCA CTCTCAAGTT
     4501 TATTTGTACT ACTGGCAAGC TCCCAGTACC ATGGCCAACT CTCGTAACTA CTCTGACCTA
     4561 TGGCGTACAA TGTTTTTCCC GCTATCCAGA TCACATGAAG CAACATGATT TTTTTAAGTC
     4621 CGCAATGCCA GAGGGCTATG TACAAGAGCG CACTATTAGC TTTAAGGATG ATGGCACCTA
     4681 TAAGACTCGC GCAGAGGTAA AGTTTGAGGG CGATACTCTC GTAAATCGCA TTGAGCTCAA
     4741 GGGCATTGAT TTTAAGGAGG ATGGCAATAT TCTCGGCCAT AAGCTGGAGT ATAATTTCAA
     4801 TTCCCATAAT GTATATATTA CCGCAGATAA GCAAAAGAAT GGCATTAAGG CGAATTTTAA
     4861 GATTCGCCAT AATGTGGAGG ATGGCTCCGT ACAACTCGCA GATCATTATC AACAAAATAC
     4921 TCCAATTGGC GATGGCCCAG TACTCCTCCC AGATAATCAT TATCTCTCCA CTCAATCCGT
     4981 GCTCTCCAAA GATCCAAATG AGAAGCGCGA TCACATGGTA CTCCTGGAGT TTGTAACTGC
     5041 AGCAGGCATT ACTCATGGCA TGGATGAGCT CTATAAGCTC GAGTGATAAA CTAGTTAGAT
     5101 AAGGAGGTTA CATATGTCTA AATTGACATC TGCTGTTCCA GTTTTGACCG CTAGAGATGT
     5161 TGCTGGTGCT GTTGAATTTT GGACTGATAG ATTGGGTTTC TCCAGAGATT TCGTTGAAGA
     5221 TGATTTCGCT GGTGTTGTTA GAGATGATGT TACCTTGTTC ATTTCCGCCG TTCAAGATCA
     5281 AGTTGTTCCA GATAATACTT TGGCTTGGGT TTGGGTTAGA GGTTTGGATG AATTATATGC
     5341 CGAATGGTCC GAAGTTGTTT CTACCAATTT TAGAGATGCT TCTGGTCCAG CTATGACCGA
     5401 AATTGGTGAA CAACCTTGGG GTAGAGAATT TGCTTTGAGA GATCCAGCTG GTAATTGCGT
     5461 TCATTTTGTT GCTGAAGAAC AAGATTAATA ATCCAAACCT GTTATATGTT AGCTGAGACT
     5521 AGTTGGAAGT GTGGCTGTCC TCAAGCGTTT TAGTTCGTCG GTCAGTTTCA CCTGATTTAC
     5581 GTAAAAACCC GCTTCGGCGG GTTTTTGCTT TTGGAGGGGC AGAAAGATGA ATGACTGTCG
     5641 GCCATTCGAT GGTGTCGGGT AGCATAACCC CTTGTGATAC CTTTGCCATG TTTCAGAAAC
     5701 AACTCTGGCG CATCGGGCTT GGACCAAAAC GAAAAAAGGC CGCTTTCGCG GCCTCTTTTC
     5761 TGGAATTTGG TACCGAGCTC ACAGCGGCGA TAGTCAGATA GCTAGACCGT ATGTTACCGA
     5821 GCCTGCACTC CTCGAATTCT TAGGATTATT ACTGCTCTTC GCGCGTAAGT GCGCGCCACA
     5881 TAGCCTCGAA GCCCAACGCA ATGTACTCAC CAGCGCGAGC CGGGTTGCGC GCAGCGAAAT
     5941 CCATAGTCGT CTCAGCAAGC GCCAAGAACA ACCCGTCGCC GAAGGCGCGG TACTCGTCGG
     6001 ACATAAACAC CATAAGAACC TCACGGTGGA CGATGTCGCG TAACTCCGGG AACATATCAT
     6061 CCGCGCGTTG TTCGGTCTCC TTCGTCAACT TTTCAGAAAC CGCCAACTGA CGTATGGCAC
     6121 GATGGCGAGC TGGGTGGTTC AATCCCCAGC TAATGATACT GTTCCAGAGA AAACGCGACA
     6181 TCATCTTAGC GTCAGTAATA GAACGATCCA ATTCCATGAT GGATGATTGG CACCAGTCCT
     6241 GGAACAAATG TAAGTAAAGG GTGTTGATCA ACTCATCTTT CGTTGCGAAA TAGCGGAACA
     6301 ACGTCCCTTC CGCAACTCCC GCATTGCGTG CAATTACAGC GGTACTAGCG GCAATGCCTG
     6361 ATTGCGCGAT GGCTTGAGTT GCCGCTTCAA GCAATGCCTG CTTTTTGTCC TCAGACTTTG
     6421 GGCGAGCAAC CATATGTAGG CCTCGTGGTT CACTAGTTTA AACAAAATTA
//