LOCUS       pReg-FEN2               5697 bp ds-DNA     circular     14-OCT-2022
DEFINITION  .
FEATURES             Location/Qualifiers
     Promoter        complement(139..213)
                     /label="RiboJ"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     Terminator      complement(363..425)
                     /label="L3S2P00 terminator"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
     misc_feature    745..1704
                     /label="LacI"
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_fwdcolor="#85dae9"
     misc_feature    1964..2824
                     /label="AmpR"
                     /ApEinfo_revcolor="#75c6a9"
                     /ApEinfo_fwdcolor="#75c6a9"
     misc_feature    2979..3597
                     /label="pBR322"
                     /ApEinfo_revcolor="#faac61"
                     /ApEinfo_fwdcolor="#faac61"
     misc_feature    complement(4004..4195)
                     /label="ROP protein"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
     Terminator      complement(5123..5179)
                     /label="L3S2P22 terminator"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
     misc_feature    complement(5251..114)
                     /label="FEN2"
                     /ApEinfo_revcolor="#c7b0e3"
                     /ApEinfo_fwdcolor="#c7b0e3"
ORIGIN
        1 AGTTGGAGGA GTAACACCCA CTTCGGCGCA GATTGCACGC ATGGTAGCTC CGTCGCGGCC
       61 CTTGGCGCTA AGTAAGCGCA TAGCTGCGTG AAGTAACGAC TGCTTAATGT CCATATGTGC
      121 GGGTCTGCGG CAGCTAGTTT AAACAAAATT ATTTGTAGAG GCTGTTTCGT CCTCACGGAC
      181 TCATCAGACC GGAAAGCACA TCCGGTGACA GCTGTGCTCA GTATCTTGAT TGCTAGCACT
      241 ATACCTAGGA CTGAGCTAGC CGTAAAGGTA CTTTTCAAAG GTCGACAGCC CATGCCCTTG
      301 CGTTCGGCAG GTGTACAATG ATACGAGGTA ATGAAGATGA AGTCCATACA ATCGATAGAT
      361 TGGGACCAAA ACGAAAAAAG GGGAGCGGTT TCCCGCTCCC CTCTTTTCTG GAATTTGGTA
      421 CCGAGTCGCA CCTGATTGCC CGACATTATC GCACGGTGTC TCATCTCTGA TAACGCTGTC
      481 TTCGCGGCCG CAACAAATAG GGGTTCCGCG CACATTTCCC CGAAAAGTGC CACCTGACGT
      541 CCCGACACCA TCGAATGGTG CAAAACCTTT CGCGGTATGG CATGATAGCG CCCGGAAGAG
      601 AGTCAATTCA GGGTGGTGAA TGTGAAACCA GTAACGTTAT ACGATGTCGC AGAGTATGCC
      661 GGTGTCTCTT ATCAGACCGT TTCCCGCGTG GTGAACCAGG CCAGCCACGT TTCTGCGAAA
      721 ACGCGGGAAA AAGTGGAAGC GGCGATGGCG GAGCTGAATT ACATTCCCAA CCGCGTGGCA
      781 CAACAACTGG CGGGCAAACA GTCGTTGCTG ATTGGCGTTG CCACCTCCAG TCTGGCCCTG
      841 CACGCGCCGT CGCAAATTGT CGCGGCGATT AAATCTCGCG CCGATCAACT GGGTGCCAGC
      901 GTGGTGGTGT CGATGGTAGA ACGAAGCGGC GTCGAAGCCT GTAAAGCGGC GGTGCACAAT
      961 CTTCTCGCGC AACGCGTCAG TGGGCTGATC ATTAACTATC CGCTGGATGA CCAGGATGCC
     1021 ATTGCTGTGG AAGCTGCCTG CACTAATGTT CCGGCGTTAT TTCTTGATGT CTCTGACCAG
     1081 ACACCCATCA ACAGTATTAT TTTCTCCCAT GAAGATGGTA CGCGACTGGG CGTGGAGCAT
     1141 CTGGTCGCAT TGGGTCACCA GCAAATCGCG CTGTTAGCGG GCCCATTAAG TTCTGTCTCG
     1201 GCGCGTCTGC GTCTGGCTGG CTGGCATAAA TATCTCACTC GCAATCAAAT TCAGCCGATA
     1261 GCGGAACGGG AAGGCGACTG GAGTGCCATG TCCGGTTTTC AACAAACCAT GCAAATGCTG
     1321 AATGAGGGCA TCGTTCCCAC TGCGATGCTG GTTGCCAACG ATCAGATGGC GCTGGGCGCA
     1381 ATGCGCGCCA TTACCGAGTC CGGGCTGCGC GTTGGTGCGG ATATCTCGGT AGTGGGATAC
     1441 GACGATACCG AAGATAGCTC ATGTTATATC CCGCCGTTAA CCACCATCAA ACAGGATTTT
     1501 CGCCTGCTGG GGCAAACCAG CGTGGACCGC TTGCTGCAAC TCTCTCAGGG CCAGGCGGTG
     1561 AAGGGCAATC AGCTGTTGCC CGTCTCACTG GTGAAAAGAA AAACCACCCT GGCGCCCAAT
     1621 ACGCAAACCG CCTCTCCCCG CGCGTTGGCC GATTCATTAA TGCAGCTGGC ACGACAGGTT
     1681 TCCCGACTGG AAAGCGGGCA GTGAGCGCAA CGCAATTAAT GTAAGTTAGC TCACTCATTA
     1741 GGCACAATTC TCATGTTTGA CAGCTTATCA TCGACTGCAC GGTGCACCAA TGCTTCTGGC
     1801 GTCAGGCAGC CATCGGAAGC TGTGGTATGG CTGTGCAGGT CGTAAATCAC TGCATAATTC
     1861 GTGTCGCTCA AGGCGCATAT TTTTCTAAAT ACATTCAAAT ATGTATCCGC TCATGAGACA
     1921 ATAACCCTGA TAAATGCTTC AATAATATTG AAAAAGGAAG AGTATGAGTA TTCAACATTT
     1981 CCGTGTCGCC CTTATTCCCT TTTTTGCGGC ATTTTGCCTT CCTGTTTTTG CTCACCCAGA
     2041 AACGCTGGTG AAAGTAAAAG ATGCTGAAGA TCAGTTGGGT GCACGAGTGG GTTACATCGA
     2101 ACTGGATCTC AACAGCGGTA AGATCCTTGA GAGTTTTCGC CCCGAAGAAC GTTTTCCAAT
     2161 GATGAGCACT TTTAAAGTTC TGCTATGTGG CGCGGTATTA TCCCGTGTTG ACGCCGGGCA
     2221 AGAGCAACTC GGTCGCCGCA TACACTATTC TCAGAATGAC TTGGTTGAGT ACTCACCAGT
     2281 CACAGAAAAG CATCTTACGG ATGGCATGAC AGTAAGAGAA TTATGCAGTG CTGCCATAAC
     2341 CATGAGTGAT AACACTGCGG CCAACTTACT TCTGACAACG ATCGGAGGAC CGAAGGAGCT
     2401 AACCGCTTTT TTGCACAACA TGGGGGATCA TGTAACTCGC CTTGATCGTT GGGAACCGGA
     2461 GCTGAATGAA GCCATACCAA ACGACGAGCG TGACACCACG ATGCCTGCAG CAATGGCAAC
     2521 AACGTTGCGC AAACTATTAA CTGGCGAACT ACTTACTCTA GCTTCCCGGC AACAATTAAT
     2581 AGACTGGATG GAGGCGGATA AAGTTGCAGG ACCACTTCTG CGCTCGGCCC TTCCGGCTGG
     2641 CTGGTTTATT GCTGATAAAT CTGGAGCCGG TGAGCGTGGA TCGCGCGGTA TCATTGCAGC
     2701 ACTGGGGCCA GATGGTAAGC CCTCCCGTAT CGTAGTTATC TACACGACGG GGAGTCAGGC
     2761 AACTATGGAT GAACGAAATA GACAGATCGC TGAGATAGGT GCCTCACTGA TTAAGCATTG
     2821 GTAACTGTTA GACCAAGTTT ACTCATATAT ACTTTAGATT GATTTAAAAC TTCATTTTTA
     2881 ATTTAAAAGG ATCTAGGTGA AGATCCTTTT TGATAATCTC ATGACCAAAA TCCCTTAACG
     2941 TGAGTTTTCG TTCCACTGAG CGTCAGACCC CGTAGAAAAG ATCAAAGGAT CTTCTTGAGA
     3001 TCCTTTTTTC TGCGCGTAAT CTGCTGCTTG CAAACAAAAA AACCACCGCT ACCAGCGGTG
     3061 GTTTGTTTGC CGGATCAAGA GCTACCAACT CTTTTTCCGA AGGTAACTGG CTTCAGCAGA
     3121 GCGCAGATAC CAAATACTGT CCTTCTAGTG TAGCCGTAGT TAGGCCACCA CTTCAAGAAC
     3181 TCTGTAGCAC CGCCTACATA CCTCGCTCTG CTAATCCTGT TACCAGTGGC TGCTGCCAGT
     3241 GGCGATAAGT CGTGTCTTAC CGGGTTGGAC TCAAGACGAT AGTTACCGGA TAAGGCGCAG
     3301 CGGTCGGGCT GAACGGGGGG TTCGTGCACA CAGCCCAGCT TGGAGCGAAC GACCTACACC
     3361 GAACTGAGAT ACCTACAGCG TGAGCTATGA GAAAGCGCCA CGCTTCCCGA AGGGAGAAAG
     3421 GCGGACAGGT ATCCGGTAAG CGGCAGGGTC GGAACAGGAG AGCGCACGAG GGAGCTTCCA
     3481 GGGGGAAACG CCTGGTATCT TTATAGTCCT GTCGGGTTTC GCCACCTCTG ACTTGAGCGT
     3541 CGATTTTTGT GATGCTCGTC AGGGGGGCGG AGCCTATGGA AAAACGCCAG CAACGCGGCC
     3601 TTTTTACGGT TCCTGGCCTT TTGCTGGCCT TTTGCTCACA TGTTCTTTCC TGCGTTATCC
     3661 CCTGATTCTG TGGATAACCG TATTACCGCC TTTGAGTGAG CTGATACCGC TCGCCGCAGC
     3721 CGAACGACCG AGCGCAGCGA GTCAGTGAGC GAGGAAGCGG AAGAGCGCCT GATGCGGTAT
     3781 TTTCTCCTTA CGCATCTGTG CGGTATTTCA CACCGGTGCA CTCTCAGTAC AATCTGCTCT
     3841 GATGCCGCAT AGTTAAGCCA GTATACACTC CGCTATCGCT ACGTGACTGG GTCATGGCTG
     3901 CGCCCCGACA CCCGCCAACA CCCGCTGACG CGCCCTGACG GGCTTGTCTG CTCCCGGCAT
     3961 CCGCTTACAG ACAAGCTGTG ACCGTCTCCG GGAGCTGCAT GTGTCAGAGG TTTTCACCGT
     4021 CATCACCGAA ACGCGCGAGG CAGCTGCGGT AAAGCTCATC AGCGTGGTCG TGAAGCGATT
     4081 CACAGATGTC TGCCTGTTCA TCCGCGTCCA GCTCGTTGAG TTTCTCCAGA AGCGTTAATG
     4141 TCTGGCTTCT GATAAAGCGG GCCATGTTAA GGGCGGTTTT TTCCTGTTTG GTCACTGATG
     4201 CCTCCGTGTA AGGGGGATTT CTGTTCATGG GGGTAATGAT ACCGATGAAA CGAGAGAGGA
     4261 TGCTCACGAT ACGGGTTACT GATGATGAAC ATGCCCGGTT ACTGGAACGT TGTGAGGGTA
     4321 AACAACTGGC GGTATGGATG CGGCGGGACC AGAGAAAAAT CACTCAGGGT CAATGCCAGC
     4381 GCTTCGTTAA TACAGATGTA GGTGTTCCAC AGGGTAGCCA GCAGCATCCT GCGATGCAGA
     4441 TCCGGAACAT AATGGTGCAG GGCGCTGACT TCCGCGTTTC CAGACTTTAC GAAACACGGA
     4501 AACCGATGAC CATTCATGTT GTTGCTCAGG TCGCAGACGT TTTGCAGCAG CAGTCACTTC
     4561 ACGTTCGCTC GCGTATCGGT GATTCATTCT GCTAACCAGT AAGGCAACCC CGCCAGCCTA
     4621 GCCGGGTCCT CAACGACAGG AGCACGATCA TGCGCACCCG TGGCCAGGAC CCAACGCTGC
     4681 CCGAGATGCG CCGCGTGCGG CTGCTGGAGA TGGCGGACGC GATGGATATG TTCTGCCAAG
     4741 GGTTGGTTTG CGCATTCACA GTTCTCCGCA AGAATTGATT GGCTCCAATT CTTGGAGTGG
     4801 TGAATCCGTT AGCGAGGTGC CGCCGGCTTC CATTCAGGTC GAGGTGGCCC GGCTCCATGC
     4861 ACCGCGACGC AACGCGGGGA GGCAGACAAG GTATAGGGCG GCGCCTACAA TCCATGCCAA
     4921 CCCGTTCCAT GTGCTCGCCG AGGCGGCATA AATCGCCGTG ACGATCAGCG GTCCAATGAT
     4981 CGAAGTTAGG CTGGTAAGAG CCGCGAGCGA TCCTTGAAGC TGTCCCTGAT GGTCGTCATC
     5041 TACCTGCCTG GACAGCATGG CGGCCGCGAA GACTAGTGAT ACCTTTGCCA TGTTTCAGAA
     5101 ACAACTCTGG CGCATCGGGC TTGGACCAAA ACGAAAAAAG GCCGCTTTCG CGGCCTCTTT
     5161 TCTGGAATTT GGTACCGAGC TCACAGCGGC GATAGTCAGA TAGCTAGACC GTATGTTACC
     5221 GAGCCTGCAC TCCTCGAATT CTTAGGATTA TTATTCCTCC TCGATATTAA ACAGTGCTAC
     5281 AAGTGTAATA TCTAATTTAG CCTTCTGCAG TGCCAAGTCC TGAGTGTAAC CTGCTCCTTC
     5341 ACGTGAAAGT GCATAGGTCA GTGCACCCAA AGCGCCCATC CATAATAATT GGGCAGTCTC
     5401 ACGCGGTGGG AAGGTTAAGC GGCCTTGTGC ATGGAACTGA GCTAAATCAT CTGCGACTCC
     5461 GCGAAGGGTA TCAGCCACCA TGGAAGGGGG TTCTCCTGCC ATGATATGTT GAACCAACAT
     5521 ACGGCACATA TTTGGCTCTG AATAGGCGAA CTGCAGGAAT GTGGCCCAGC CGTCGCGGAT
     5581 GCCTTTTAAC GGTCCACGCT CCTCGGTTCC GCCGTGATAT GCCTCTGCGA CCTGACGGAA
     5641 TGTTTCATCG ATAGCTGCTT TATGTAAACC TTGAAGATCG CCATAATGGT GATACAG
//