LOCUS       dora[A]_rescue_c        8953 bp DNA     circular SYN 09-AUG-2022
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8953)
  AUTHORS   Glick Lab
  TITLE     Direct Submission
  JOURNAL   Exported Aug 10, 2022 from SnapGene 6.1.0
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..8953
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             2864..5695
                     /codon_start=1
                     /translation="DRFSFDDSIRFEEDSLCSWSSEPESLCNNWRGWKKPAAGSGAGNG
                     GLSGMGGMAVAGGSGAPPFGGAGASGSCTPGGNGTAMANTSTCGRHYEVSTLAELAARC
                     VASYIPFELVEHVYPPVPEQLQLRIAFWSFPDNEEDIRLYSCLANSSADEFNRGDSLFR
                     LRAVKDPLQIGFHLSASVVNQTPRAYFNVAVTFDRRRISSCNCTCTSSAYWCSHVVAVC
                     LHRIHCPQEVCLRAPVSESLTRLQRDQLQKFAQYLISELPQQILPTAQRLLDELLSAQP
                     TAINTVCGAPDPTAGASINDQTSWYLDEKTLHNNIKRILIKFCLPAPIVFSDVNYLTNS
                     APPAAAEWSSLLRPLRGREPEGMWNLLSIVREMYRRCDRNAVRLLEIITEECLYCDQIL
                     IWWFQTKLALMMGSHGHSGGKHSNTHSNSTALQHACSSLCDEIVALWRLAALNPGLAPD
                     ERDMLHAQFTAWHLKILDRVVKSRMMPSYTNKHQQNSRSETELFIGFKPAIEACYLDWE
                     GYPIPGVTHTHDTNPIYYSPFTCFKHTDLKGESNNPGQLNATQALMSNNKHYNYFSSSS
                     DHGMHAGAFKQRLDRPFRESRFYTNPADLDGGAALAGGGSGRFSTGLGSVAVGGAGAGG
                     LGQMSGGVNNSVGGSAAAEVNPVQQQLPSGSAGVGASVTNAKVVVATDGNRSSASSEGF
                     CENDDFGGDTSSSHNYCPPGQAVVPGQKSALTESDSQSSFDAVSHQSKDEPPVGGVGVA
                     VGVEQALSTSDTSSASSSSSSASTASGSSNSSTSSMLMAGQEATVGVGGAVQQTTRRLS
                     KDESFSSSSDEFNQGGVGGSAGRVAMASSGSGSGAGDTVAVGSGSTAGGDLTPVPSTSA
                     AARAALAASSTGPVISASPHLAASAAGGLGVLGVGADQPCTSSAHAARALAAAVAASSD
                     KPHVFSNVRPTEDA"
     misc_feature    2864..3140
                     /label=Exon1
     misc_feature    3141..3383
                     /label=Exon2
     misc_feature    3384..3547
                     /label=Exon3
     misc_feature    3548..3858
                     /label=Exon4
     misc_feature    3859..5695
                     /label=Exon5
     CDS             5738..5791
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             5816..6415
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     polyA_signal    6423..6557
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     rep_origin      complement(7158..7746)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(7917..8777)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(8778..8882)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 ggcgtatcac gaggcccttt cgtcttcaag aattaattct atattctaaa aacacaaatg
       61 atacttctaa aaaaaaatca tgaatggcat caactctgaa tcaaatcttt gcagatgcac
      121 ctacttctca tttccactgt cacatcattt ttccagatct aaaaaagcac cgactcggtg
      181 ccactttttc aagttgataa cggactagcc ttattttaac ttgctatttc tagctctaaa
      241 actacataac gaataaaaaa ggatttaagt gcaaatggta aaaaagtgtc gagtttttct
      301 tgagttgatt gtgctgtaat gagactctgc attcggcatt tgactcggct tttcctactc
      361 gtgccgtatt tcaggctgca agtcgaacag atctcgctgc ctgttatgtg gcccacaaac
      421 caagacacgt tttatggcca ttaaagctgg ctgatcgtcg ccaaacacca aatacataat
      481 gaatatgtac acattcgaga aagaagcgat caaagaagcg tcttcgggcg gagtaggaga
      541 atgcggagga gaaggagaac gagctgatct agtatctctc cacaatccaa tgccaactga
      601 ccaactggcc atattcggag caatttgaag ccaatttcca tcgcctggcg atcgctccat
      661 tcttggctat atgtttttca ccgttacccg gggccatttt caaagactcg tcggcaagat
      721 aagattgtgt cactcgctgt ctctcttcat ttgtcgaaga atgctgagga atttcgcgat
      781 gacgtcggcg agtattttga agaatgagaa taatttgtat ttatacgaaa atcagttagt
      841 ggaattttct acaaaaacat gttatctata gataattttg ttgcaaaata tgttgactat
      901 gacaaagatt gtatgtatat ctttaatgta ttctcatttt cttatgtatt tataatggca
      961 atgatgatac tgatgatatt ttaagatgat gccagaccaa aaggcttgaa tttctgcgtc
     1021 ttttgccgaa cgcagtgcat gtgcaattgt tgttttttgg aatattcaat tttcggactg
     1081 tccgctttga tttcagtttc ttggcttatt caaaaagcaa agtaaagcca aaaaagcgag
     1141 atggcaatac caaatgcggc aaaacggtag tggaaggaaa ggggtgcggg gcagcggaag
     1201 gaagggtggg gcggggcgtg gcggggtctg tggctgggcg cgacgtcacc gacgttggag
     1261 ccactccttt gaccatgtgt gcgtgtgtgt attattcgtg tctcgccact cgccggttgt
     1321 ttttttcttt ttatgctgcg ctctctctag cgccatctcg cttacgcatg ctcaacgcac
     1381 cgcatgttgc cgtttccttt tatgcgtcat tttggctcga aataggcaat tatttaaaca
     1441 aagattagtc aacgaaaacg ctaaaataaa taagtctaca atatggttac ttattgccat
     1501 gtgtgtgcag ccaacgatag caacaaaagc aacaacacag gtggctttcc ctctttcact
     1561 ttttgtttgc aagccgcgtg cgagcaagac ggcacgaccg gcaaacgcaa ttacgctgac
     1621 aaagagcaga cgaagttttg gcgaaaaaca tcaaggcgcc tgatacgaat gcatttgcaa
     1681 taacaattgc gatatttaat attgtttatg aagctgtttg acttcaaaac acacaaaaaa
     1741 aaaaataaaa caaattattt gaaagagaat taggaatcgg acgcttatcg ttagggtaac
     1801 aacaagaaat gcttactgag tcacagcctc tggaaaactg ccgcaagcca gagagagaga
     1861 gaaaaagagg gagagcagct tagaccgcat gtgcttgtgt gtgaggcgtc tctctcttcg
     1921 tctctgttgc gcaaacgcat agactgcact gaaaaaatcg attacctatt ttttatgaat
     1981 gaatatttgc actattacta ttcaaaacta ttaagatagc aatcacattc aatagccaaa
     2041 tactatacca cctgagcgat gcaacgaaat gatcaatttg agcaaaaatg ctgcatattt
     2101 aggacggcat cattatagaa atgcttcttg ctgtgtactt ttctctcgtc tggcagctgt
     2161 ttcgccgtta ttgttaaaac cggcttaagt taggtgtgtt ttctacgact agtgaatgcc
     2221 ctactagaag atgtgtgttg cacaaaatgt ccctggaata accaatttga agtgcagata
     2281 gcagtaaacg taagctaata tgaatattat ttaactgtaa tgttttaata tcgctggaca
     2341 ttactaataa acccactata aacacatgta catatgtatg ttttggcata caatgagtag
     2401 ttggggaaaa aatgtgtaaa agcaccgtga ccatcacagc ataaagataa ccagctgaag
     2461 tatcgaatat gagtaacccc caaattgaat cacatgccgc aactgatagg acccatggaa
     2521 gtacactctt catggcgata tacaagacac acacaagcac gaacacccag ttgcggagga
     2581 aattctccgt aaatgaaaac ccaatcggcg aacaattcat acccatatat ggtaaaagtt
     2641 ttgaacgcga cttgagagcg gagagcattg cggctgataa ggttttagcg ctaagcgggc
     2701 tttataaaac gggctgcggg accagttttc atatcactac cgtttgagtt cttgtgctgt
     2761 gtggatactc ctcccgacac aaagccgctc catcagccag cagtcgtcta atccagagac
     2821 cccggatcgg ggtacctact agtccagtgt ggtggaattc atggaccgct tcagcttcga
     2881 cgactccatc cgcttcgagg aagactccct ctgcagctgg agctctgaac cggaatcgct
     2941 gtgcaacaac tggcgcggct ggaagaagcc cgctgcaggc agcggcgcag gcaatggcgg
     3001 cttgagcggc atgggcggta tggctgtggc cggcggctca ggagcaccgc cgttcggagg
     3061 agcaggagcc tctggttcct gcacaccggg cggcaatgga acggccatgg ccaacacatc
     3121 cacctgcggc agacattatg aagtatccac attggccgaa ctggcagccc gctgtgtggc
     3181 ctcctacata ccgttcgagc tggttgagca cgtgtatccg ccggtgccgg agcagctgca
     3241 gctgcgcatt gcgttctgga gctttccgga caacgaggag gacatccggc tctactcctg
     3301 tctggccaat agctcggcgg acgagttcaa tcgcggcgac tcgttgtttc ggttgcgagc
     3361 tgtcaaggat ccgctgcaga taggctttca cctctcggcc agcgttgtca atcagacgcc
     3421 gcgtgcctat ttcaatgtgg ccgtcacctt tgaccgccga cgcatctcct cttgcaactg
     3481 cacctgcacc tcgtccgcct actggtgctc ccacgtagtc gccgtttgtc tgcatcgcat
     3541 ccattgcccc caggaggtct gtctgcgtgc cccggtatcg gaatcattga ctcgcctgca
     3601 acgcgaccaa ttgcaaaagt ttgcccagta tctgatcagc gagctgcctc agcagatctt
     3661 gccgacggct cagcggctcc tggatgagtt gctcagtgcg caacccacgg cgatcaacac
     3721 tgtgtgcggt gcacctgatc ccacggctgg ggcctcgatc aacgaccaga ccagctggta
     3781 tctggacgag aagacgttgc ataacaacat taaacgaatc ctcatcaagt tctgtttgcc
     3841 cgccccgatt gtgtttagcg acgtaaacta tctgacgaac tcggctcccc cagcggcggc
     3901 cgaatggagc tcgctactcc gaccgctgcg gggacgtgag ccggagggca tgtggaatct
     3961 actttcgatc gtacgcgaga tgtacaggcg ctgtgatcgg aacgcagtgc gtctgctgga
     4021 gatcatcacg gaggagtgct tgtactgcga tcagatactc atctggtggt tccagacgaa
     4081 gttggcactg atgatggggt cccacggcca ctccggcggt aagcattcta acactcactc
     4141 caactccacg gcactgcagc atgcttgcag ttcgctgtgc gacgaaatcg tggcactgtg
     4201 gcgattggca gcgctcaatc ccggcctggc gccggatgag cgagatatgc tgcacgctca
     4261 gttcacggcc tggcatctaa agattcttga ccgggtggtc aagagccgaa tgatgccgtc
     4321 gtacaccaac aagcaccagc agaactcgcg atccgagacg gagctgttta tcggcttcaa
     4381 gccggccatc gaggcgtgct acttggactg ggagggctac cccataccgg gagtgacgca
     4441 cacccacgac acgaatccca tctactactc gccgttcact tgcttcaagc acacggacct
     4501 caagggcgag tctaacaatc ccggccagct gaatgccacc caagcgctga tgtcgaacaa
     4561 caagcactac aattacttta gttcatccag cgatcatggc atgcacgcag gagccttcaa
     4621 gcagaggttg gaccggcctt ttcgtgagtc gcgattctat acgaatccgg ccgatctgga
     4681 cggaggagca gcgttggcgg gcggaggatc cggtaggttt tccacgggac tgggctccgt
     4741 tgccgttgga ggagctggtg caggaggctt gggtcagatg agcggaggag ttaacaactc
     4801 tgttggagga tcagcggccg ccgaagtgaa tcccgtgcag cagcagcttc ccagtggctc
     4861 ggcgggagtc ggtgcctctg tgacaaatgc caaggttgtg gtagcaacag atggcaatcg
     4921 ttcgagtgcc agcagcgagg gattctgcga gaacgacgat ttcggcggcg acaccagcag
     4981 cagtcacaac tactgtccac ctggacaggc ggttgtgcct ggccagaaat cagccctcac
     5041 ggagtcggat tcgcagagca gcttcgatgc agtgtcgcat cagagcaagg atgagccacc
     5101 agtcggggga gtgggtgtgg cagtgggtgt tgaacaagcc ctttccacat ctgacacttc
     5161 gtcggcctcc tcctcatcgt catctgcttc tacagcttca gggagctcta atagttccac
     5221 ttcctctatg cttatggctg gacaggaggc tactgtcggt gttggtggcg ctgtgcagca
     5281 aacaactcgt cgcctcagca aggatgaatc ctttagtagc agtagcgacg agttcaacca
     5341 gggaggcgtg ggtggatcag caggtcgagt ggctatggcc tccagtggaa gcggatcggg
     5401 tgctggcgac acagtagcgg tgggatcggg atcaacagct ggcggagatc tgacgccggt
     5461 gcccagcacg tcggcagctg cccgtgctgc cttggccgcc agctcgacag ggccggtgat
     5521 ttctgcttca cctcatctcg cagctagcgc cgctggaggc ttaggagtgc tgggtgtggg
     5581 tgcagaccag ccttgcacct catccgcgca tgcagcccga gcactggcag ctgcagtggc
     5641 tgcttccagc gataagccgc atgttttctc caatgtccgg cccacagagg acgccaaggc
     5701 cggccaggca aaaaagaaaa agggatcagg caagcttgag ggcagaggaa gtcttctaac
     5761 atgcggtgac gtggaggaga atcccggccc tgctagcggt agcggcagcg gtagcatgac
     5821 cgagtacaag cccacggtgc gcctcgccac ccgcgacgac gtcccccggg ccgtacgcac
     5881 cctcgccgcc gcgttcgccg actaccccgc cacgcgccac accgtcgacc cggaccgcca
     5941 catcgagcgg gtcaccgagc tgcaagaact cttcctcacg cgcgtcgggc tcgacatcgg
     6001 caaggtgtgg gtcgcggacg acggcgccgc ggtggcggtc tggaccacgc cggagagcgt
     6061 cgaagcgggg gcggtgttcg ccgagatcgg cccgcgcatg gccgagttga gcggttcccg
     6121 gctggccgcg cagcaacaga tggaaggcct cctggcgccg caccggccca aggagcccgc
     6181 gtggttcctg gccaccgtcg gcgtctcgcc cgaccaccag ggcaagggtc tgggcagcgc
     6241 cgtcgtgctc cccggagtgg aggcggccga gcgcgccggg gtgcccgcct tcctggagac
     6301 ctccgcgccc cgcaacctcc ccttctacga gcggctcggc ttcaccgtca ccgccgacgt
     6361 cgaggtgccc gaaggaccgc gcacctggtg catgacccgc aagcccggtg cctgactcga
     6421 gggatccaga catgataaga tacattgatg agtttggaca aaccacaact agaatgcagt
     6481 gaaaaaaatg ctttatttgt gaaatttgtg atgctattgc tttatttgta accattataa
     6541 gctgcaataa acaagttaac aacaacaatt gcattcattt tatgtttcag gttcaggggg
     6601 aggtgtggga ggttttttaa agcaagtaaa acctctacaa atgtggtatg gctgattatg
     6661 atcagtcgac cgatgccctt gagagccttc aacccagtca gctccttccg gtgggcgcgg
     6721 ggcatgacta tcgtcgccgc acttatgact gtcttcttta tcatgcaact cgtaggacag
     6781 gtgccggcag cgctctgggt cattttcggc gaggaccgct ttcgctggag cgcgacgatg
     6841 atcggcctgt cgcttgcggt attcggaatc ttgcacgccc tcgctcaagc cttcgtcact
     6901 ggtcccgcca ccaaacgttt cggcgagaag caggccatta tcgccggcat ggcggccgac
     6961 gcgctgggct acgtcttgct ggcgttcgcg acgcgaggct ggatggcctt ccccattatg
     7021 attcttctcg cttccggcgg catcgggatg cccgcgttgc aggccatgct gtccaggcag
     7081 gtagatgacg accatcaggg acagcttcaa ggccagcaaa aggccaggaa ccgtaaaaag
     7141 gccgcgttgc tggcgttttt ccataggctc cgcccccctg acgagcatca caaaaatcga
     7201 cgctcaagtc agaggtggcg aaacccgaca ggactataaa gataccaggc gtttccccct
     7261 ggaagctccc tcgtgcgctc tcctgttccg accctgccgc ttaccggata cctgtccgcc
     7321 tttctccctt cgggaagcgt ggcgctttct catagctcac gctgtaggta tctcagttcg
     7381 gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac cccccgttca gcccgaccgc
     7441 tgcgccttat ccggtaacta tcgtcttgag tccaacccgg taagacacga cttatcgcca
     7501 ctggcagcag ccactggtaa caggattagc agagcgaggt atgtaggcgg tgctacagag
     7561 ttcttgaagt ggtggcctaa ctacggctac actagaagga cagtatttgg tatctgcgct
     7621 ctgctgaagc cagttacctt cggaaaaaga gttggtagct cttgatccgg caaacaaacc
     7681 accgctggta gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga
     7741 tctcaagaag atcctttgat cttttctacg gggtctgacg ctcagtggaa cgaaaactca
     7801 cgttaaggga ttttggtcat gagattatca aaaaggatct tcacctagat ccttttaaat
     7861 taaaaatgaa gttttaaatc aatctaaagt atatatgagt aaacttggtc tgacagttac
     7921 caatgcttaa tcagtgaggc acctatctca gcgatctgtc tatttcgttc atccatagtt
     7981 gcctgactcc ccgtcgtgta gataactacg atacgggagg gcttaccatc tggccccagt
     8041 gctgcaatga taccgcgaga cccacgctca ccggctccag atttatcagc aataaaccag
     8101 ccagccggaa gggccgagcg cagaagtggt cctgcaactt tatccgcctc catccagtct
     8161 attaattgtt gccgggaagc tagagtaagt agttcgccag ttaatagttt gcgcaacgtt
     8221 gttgccattg ctgcaggcat cgtggtgtca cgctcgtcgt ttggtatggc ttcattcagc
     8281 tccggttccc aacgatcaag gcgagttaca tgatccccca tgttgtgcaa aaaagcggtt
     8341 agctccttcg gtcctccgat cgttgtcaga agtaagttgg ccgcagtgtt atcactcatg
     8401 gttatggcag cactgcataa ttctcttact gtcatgccat ccgtaagatg cttttctgtg
     8461 actggtgagt actcaaccaa gtcattctga gaatagtgta tgcggcgacc gagttgctct
     8521 tgcccggcgt caacacggga taataccgcg ccacatagca gaactttaaa agtgctcatc
     8581 attggaaaac gttcttcggg gcgaaaactc tcaaggatct taccgctgtt gagatccagt
     8641 tcgatgtaac ccactcgtgc acccaactga tcttcagcat cttttacttt caccagcgtt
     8701 tctgggtgag caaaaacagg aaggcaaaat gccgcaaaaa agggaataag ggcgacacgg
     8761 aaatgttgaa tactcatact cttccttttt caatattatt gaagcattta tcagggttat
     8821 tgtctcatga gcggatacat atttgaatgt atttagaaaa ataaacaaat aggggttccg
     8881 cgcacatttc cccgaaaagt gccacctgac gtctaagaaa ccattattat catgacatta
     8941 acctataaaa ata
//
