LOCUS       Exported                7418 bp DNA     circular SYN 10-AUG-2022
DEFINITION  .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7418)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Aug 10, 2022 from SnapGene Viewer 6.0.5
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..7418
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    1..1622
                     /locus_tag="CAG"
                     /label=CAG
                     /note="color: #ff4922"
     misc_feature    1719..1730
                     /locus_tag="MCS"
                     /label=MCS
                     /note="color: #00ffff"
     CDS             1741..4356
                     /label=Sema4D
                     /note="color: #ffff99; direction: RIGHT"
     misc_feature    1741..1743
                     /locus_tag="START"
                     /label=START
                     /note="color: #2aff0c"
     misc_feature    1744..1809
                     /locus_tag="signal peptide"
                     /label=signal peptide
                     /note="color: #5aff95"
     misc_feature    1810..1839
                     /locus_tag="myc"
                     /label=myc
                     /note="color: #fc743a"
     misc_feature    1946..1965
                     /locus_tag="Sema4D_N_F1"
                     /label=Sema4D_N_F1
                     /note="color: #fc5dae"
     misc_feature    complement(2108..2127)
                     /locus_tag="Sema4D_N_R1"
                     /label=Sema4D_N_R1
                     /note="color: #00ff00; direction: LEFT"
     misc_feature    2396..2415
                     /locus_tag="SEMA4D_N_F2"
                     /label=SEMA4D_N_F2
                     /note="color: #fb3895"
     misc_feature    2793..2812
                     /locus_tag="Sema4D_N_F3"
                     /label=Sema4D_N_F3
                     /note="color: #fb1ca8"
     misc_feature    3190..3209
                     /locus_tag="Sema4D_N_F4"
                     /label=Sema4D_N_F4
                     /note="color: #fb15b6"
     misc_feature    3563..3582
                     /locus_tag="Sema4D_N_F5"
                     /label=Sema4D_N_F5
                     /note="color: #fb00ac"
     misc_feature    3944..3963
                     /locus_tag="Sema4D_N_F6"
                     /label=Sema4D_N_F6
                     /note="color: #fb149e"
     misc_feature    4033..4356
                     /locus_tag="Cytoplasmic"
                     /label=Cytoplasmic
                     /note="color: #8eff38"
     misc_feature    complement(6427..7287)
                     /locus_tag="Amp"
                     /label=Amp
                     /note="color: #1f28ff; direction: LEFT"
ORIGIN
        1 gtcgacattg attattgact agttattaat agtaatcaat tacggggtca ttagttcata
       61 gcccatatat ggagttccgc gttacataac ttacggtaaa tggcccgcct ggctgaccgc
      121 ccaacgaccc ccgcccattg acgtcaataa tgacgtatgt tcccatagta acgccaatag
      181 ggactttcca ttgacgtcaa tgggtggact atttacggta aactgcccac ttggcagtac
      241 atcaagtgta tcatatgcca agtacgcccc ctattgacgt caatgacggt aaatggcccg
      301 cctggcatta tgcccagtac atgaccttat gggactttcc tacttggcag tacatctacg
      361 tattagtcat cgctattacc atgggtcgag gtgagcccca cgttctgctt cactctcccc
      421 atctcccccc cctccccacc cccaattttg tatttattta ttttttaatt attttgtgca
      481 gcgatggggg cggggggggg gggggcgcgc gccaggcggg gcggggcggg gcgaggggcg
      541 gggcggggcg aggcggagag gtgcggcggc agccaatcag agcggcgcgc tccgaaagtt
      601 tccttttatg gcgaggcggc ggcggcggcg gccctataaa aagcgaagcg cgcggcgggc
      661 gggagtcgct gcgttgcctt cgccccgtgc cccgctccgc gccgcctcgc gccgcccgcc
      721 ccggctctga ctgaccgcgt tactcccaca ggtgagcggg cgggacggcc cttctcctcc
      781 gggctgtaat tagcgcttgg tttaatgacg gctcgtttct tttctgtggc tgcgtgaaag
      841 ccttaaaggg ctccgggagg gccctttgtg cgggggggag cggctcgggg ggtgcgtgcg
      901 tgtgtgtgtg cgtggggagc gccgcgtgcg gcccgcgctg cccggcggct gtgagcgctg
      961 cgggcgcggc gcggggcttt gtgcgctccg cgtgtgcgcg aggggagcgc ggccgggggc
     1021 ggtgccccgc ggtgcggggg ggctgcgagg ggaacaaagg ctgcgtgcgg ggtgtgtgcg
     1081 tgggggggtg agcagggggt gtgggcgcgg cggtcgggct gtaacccccc cctgcacccc
     1141 cctccccgag ttgctgagca cggcccggct tcgggtgcgg ggctccgtgc ggggcgtggc
     1201 gcggggctcg ccgtgccggg cggggggtgg cggcaggtgg gggtgccggg cggggcgggg
     1261 ccgcctcggg ccggggaggg ctcgggggag gggcgcggcg gccccggagc gccggcggct
     1321 gtcgaggcgc ggcgagccgc agccattgcc ttttatggta atcgtgcgag agggcgcagg
     1381 gacttccttt gtcccaaatc tggcggagcc gaaatctggg aggcgccgcc gcaccccctc
     1441 tagcgggcgc gggcgaagcg gtgcggcgcc ggcaggaagg aaatgggcgg ggagggcctt
     1501 cgtgcgtcgc cgcgccgccg tccccttctc catctccagc ctcggggctg ccgcaggggg
     1561 acggctgcct tcggggggga cggggcaggg cggggttcgg cttctggcgt gtgaccggcg
     1621 gctctagagc ctctgctaac catgttcatg ccttcttctt tttcctacag ctcctgggca
     1681 acgtgctggt tattgtgctg tctcatcatt ttggcaaaga attcacccgg gctagccacc
     1741 atgaggatgt gtgcccccgt tagggggctg ttcttggccc tggtggtagt gttgagaacc
     1801 gcggtggcag aacagaaact gattagcgaa gaagatctgt ttgcacctgt gcctcggctc
     1861 acctgggaac atggagaggt aggtctggtg cagtttcaca agccaggcat ctttaactac
     1921 tcggccttgc tgatgagtga ggacaaagac actctgtatg taggcgcccg ggaagcagtc
     1981 tttgcagtga atgcgctgaa catctctgag aagcaacatg aggtatattg gaaggtctct
     2041 gaagacaaaa aatccaagtg tgcagagaag gggaaatcaa agcagacgga atgcctaaac
     2101 tacattcgag tactacagcc actaagcagc acttccctct atgtgtgtgg gaccaatgcg
     2161 ttccagccca cctgtgacca cctgaacttg acatccttca agtttctggg gaaaagtgaa
     2221 gatggcaaag gaagatgccc cttcgacccc gcccacagct acacatcagt catggttggg
     2281 ggcgagctct actctgggac gtcctataat ttcttgggca gtgaacccat catctctcga
     2341 aactcttccc acagtccctt gaggacggag tatgccatcc cgtggctgaa cgagcctagc
     2401 ttcgtctttg ctgacgtgat ccagaaaagc ccagatggtc cggagggtga agatgacaag
     2461 gtctacttct tttttacgga ggtatccgtg gagtacgaat tcgtcttcaa gttgatgatc
     2521 ccgcgagttg ccagggtgtg caagggcgac cagggcggcc tgcggacttt gcaaaaaaag
     2581 tggacctcct tcctaaaggc caggctgatc tgctccaagc cagacagtgg cctggtcttc
     2641 aacatacttc aggatgtgtt tgtgctgagg gccccgggcc tcaaggagcc tgtgttctat
     2701 gcggtcttca ccccacagct gaacaatgtg ggtctgtcag cggtgtgcgc ctacacactg
     2761 gccacggtgg aggcagtctt ctcccgtgga aagtacatgc agagtgccac agtggagcag
     2821 tctcacacca agtgggtgcg ctacaatggc ccagtgccca ctccccgacc tggagcgtgt
     2881 atcgacagtg aggcccgggc agccaactac accagctcct tgaatctccc agacaaaaca
     2941 ctgcagtttg taaaagacca ccctttgatg gatgactcag tgaccccgat agacaacaga
     3001 cccaagctga tcaaaaaaga tgtaaactac acccagatag tggtagacag gacccaggcc
     3061 ctggatggga ctttctacga cgtcatgttc atcagcacag accggggagc tctgcataaa
     3121 gcagtcatcc tcacaaaaga ggtgcatgtc atcgaggaga cccaactctt ccgggactct
     3181 gaaccggtcc taactctgct gctatcgtca aagaagggga ggaagtttgt ctatgcaggc
     3241 tccaactctg gagtggtcca agcgcccctg gcattctgcg aaaagcacgg tagctgtgaa
     3301 gactgtgtgt tagcacggga cccctactgt gcctggagcc cagccatcaa ggcctgtgtt
     3361 accctgcacc aggaagaggc ctccagcagg ggctggattc aggacatgag cggtgacaca
     3421 tcctcatgcc tggataagag taaagaaagt ttcaaccagc attttttcaa gcacggcggc
     3481 acagcggaac tcaaatgttt ccaaaagtcc aacctagccc gggtggtatg gaagttccag
     3541 aatggcgagt tgaaggccgc aagtcccaag tacggctttg tgggcaggaa gcacctgctc
     3601 atcttcaacc tgtcggacgg agacagcggc gtgtaccagt gcctgtcaga ggaaagggtg
     3661 aggaataaaa cggtctccca gctgctggcc aagcacgttc tggaagtgaa gatggtacct
     3721 cggacccccc cctcacctac ctcagaggat gctcagacag aaggtagtaa gatcacatcc
     3781 aaaatgccgg ttgcatctac ccaggggtcc tctcccccta ccccggctct gtgggcaacc
     3841 tcccccagag ccgccaccct acctcccaag tcctcctccg gcacatcctg tgaaccaaag
     3901 atggtcatca acacggtccc ccagctccac tcagagaaga cggtgtatct caagtccagt
     3961 gacaaccgcc tgctcatgtc tctcctcctc ttcatctttg tcctcttcct ctgcctcttt
     4021 tcctacaact gctacaaggg ctacctgccc ggacagtgct taaaattccg ctcagccctg
     4081 ctgcttggaa agaaaacacc caagtcagac ttctctgacc tggagcagag tgtgaaggag
     4141 acactggtcg agcctgggag cttctcccag cagaacggcg accaccccaa gccagccctg
     4201 gatacgggct atgaaacgga gcaggacacc atcaccagca aagtccccac ggatcgtgag
     4261 gactcgcaac ggatcgatga actctctgcc cgggacaaac cgtttgatgt caagtgtgaa
     4321 ctgaagtttg cagattcgga tgctgacggg gactgagcgg ccgcactcct caggtgcagg
     4381 ctgcctatca gaaggtggtg gctggtgtgg ccaatgccct ggctcacaaa taccactgag
     4441 atctttttcc ctctgccaaa aattatgggg acatcatgaa gccccttgag catctgactt
     4501 ctggctaata aaggaaattt attttcattg caatagtgtg ttggaatttt ttgtgtctct
     4561 cactcggaag gacatatggg agggcaaatc atttaaaaca tcagaatgag tatttggttt
     4621 agagtttggc aacatatgcc atatgctggc tgccatgaac aaaggtggct ataaagaggt
     4681 catcagtata tgaaacagcc ccctgctgtc cattccttat tccatagaaa agccttgact
     4741 tgaggttaga ttttttttat attttgtttt gtgttatttt tttctttaac atccctaaaa
     4801 ttttccttac atgttttact agccagattt ttcctcctct cctgactact cccagtcata
     4861 gctgtccctc ttctcttatg aagatccctc gacctgcagc ccaagcttgg cgtaatcatg
     4921 gtcatagctg tttcctgtgt gaaattgtta tccgctcaca attccacaca acatacgagc
     4981 cggaagcata aagtgtaaag cctggggtgc ctaatgagtg agctaactca cattaattgc
     5041 gttgcgctca ctgcccgctt tccagtcggg aaacctgtcg tgccagcgga tccgcatctc
     5101 aattagtcag caaccatagt cccgccccta actccgccca tcccgcccct aactccgccc
     5161 agttccgccc attctccgcc ccatggctga ctaatttttt ttatttatgc agaggccgag
     5221 gccgcctcgg cctctgagct attccagaag tagtgaggag gcttttttgg aggcctaggc
     5281 ttttgcaaaa agctaacttg tttattgcag cttataatgg ttacaaataa agcaatagca
     5341 tcacaaattt cacaaataaa gcattttttt cactgcattc tagttgtggt ttgtccaaac
     5401 tcatcaatgt atcttatcat gtctggatcc gctgcattaa tgaatcggcc aacgcgcggg
     5461 gagaggcggt ttgcgtattg ggcgctcttc cgcttcctcg ctcactgact cgctgcgctc
     5521 ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag gcggtaatac ggttatccac
     5581 agaatcaggg gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa
     5641 ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc cgcccccctg acgagcatca
     5701 caaaaatcga cgctcaagtc agaggtggcg aaacccgaca ggactataaa gataccaggc
     5761 gtttccccct ggaagctccc tcgtgcgctc tcctgttccg accctgccgc ttaccggata
     5821 cctgtccgcc tttctccctt cgggaagcgt ggcgctttct caatgctcac gctgtaggta
     5881 tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac cccccgttca
     5941 gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag tccaacccgg taagacacga
     6001 cttatcgcca ctggcagcag ccactggtaa caggattagc agagcgaggt atgtaggcgg
     6061 tgctacagag ttcttgaagt ggtggcctaa ctacggctac actagaagga cagtatttgg
     6121 tatctgcgct ctgctgaagc cagttacctt cggaaaaaga gttggtagct cttgatccgg
     6181 caaacaaacc accgctggta gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag
     6241 aaaaaaagga tctcaagaag atcctttgat cttttctacg gggtctgacg ctcagtggaa
     6301 cgaaaactca cgttaaggga ttttggtcat gagattatca aaaaggatct tcacctagat
     6361 ccttttaaat taaaaatgaa gttttaaatc aatctaaagt atatatgagt aaacttggtc
     6421 tgacagttac caatgcttaa tcagtgaggc acctatctca gcgatctgtc tatttcgttc
     6481 atccatagtt gcctgactcc ccgtcgtgta gataactacg atacgggagg gcttaccatc
     6541 tggccccagt gctgcaatga taccgcgaga cccacgctca ccggctccag atttatcagc
     6601 aataaaccag ccagccggaa gggccgagcg cagaagtggt cctgcaactt tatccgcctc
     6661 catccagtct attaattgtt gccgggaagc tagagtaagt agttcgccag ttaatagttt
     6721 gcgcaacgtt gttgccattg ctacaggcat cgtggtgtca cgctcgtcgt ttggtatggc
     6781 ttcattcagc tccggttccc aacgatcaag gcgagttaca tgatccccca tgttgtgcaa
     6841 aaaagcggtt agctccttcg gtcctccgat cgttgtcaga agtaagttgg ccgcagtgtt
     6901 atcactcatg gttatggcag cactgcataa ttctcttact gtcatgccat ccgtaagatg
     6961 cttttctgtg actggtgagt actcaaccaa gtcattctga gaatagtgta tgcggcgacc
     7021 gagttgctct tgcccggcgt caatacggga taataccgcg ccacatagca gaactttaaa
     7081 agtgctcatc attggaaaac gttcttcggg gcgaaaactc tcaaggatct taccgctgtt
     7141 gagatccagt tcgatgtaac ccactcgtgc acccaactga tcttcagcat cttttacttt
     7201 caccagcgtt tctgggtgag caaaaacagg aaggcaaaat gccgcaaaaa agggaataag
     7261 ggcgacacgg aaatgttgaa tactcatact cttccttttt caatattatt gaagcattta
     7321 tcagggttat tgtctcatga gcggatacat atttgaatgt atttagaaaa ataaacaaat
     7381 aggggttccg cgcacatttc cccgaaaagt gccacctg
//
