LOCUS       pCAG_Sema4D_Flag        7412 bp    DNA        circular     10-AUG-2022
DEFINITION  .
ACCESSION   
VERSION     
SOURCE      .
  ORGANISM  .
COMMENT     
COMMENT     ApEinfo:methylated:1
FEATURES             Location/Qualifiers
     misc_feature    1..1622
                     /locus_tag="CAG"
                     /label="CAG"
                     /ApEinfo_label="CAG"
                     /ApEinfo_fwdcolor="#ff4922"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    4324..4347
                     /locus_tag="FLAG"
                     /label="FLAG"
                     /ApEinfo_label="FLAG"
                     /ApEinfo_fwdcolor="#ff6d1f"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    complement(6421..7281)
                     /locus_tag="Amp"
                     /label="Amp"
                     /ApEinfo_label="Amp"
                     /ApEinfo_fwdcolor="#ff0fff"
                     /ApEinfo_revcolor="#1f28ff"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    1719..1730
                     /locus_tag="MCS"
                     /label="MCS"
                     /ApEinfo_label="MCS"
                     /ApEinfo_fwdcolor="#fc9b22"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    1741..4323
                     /locus_tag="Sema4D"
                     /label="Sema4D"
                     /ApEinfo_label="Sema4D"
                     /ApEinfo_fwdcolor="cyan"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    complement(4516..4538)
                     /locus_tag="CAG rev sequencing primer (#8)"
                     /label="CAG rev sequencing primer (#8)"
                     /ApEinfo_label="CAG rev sequencing primer (#8)"
                     /ApEinfo_fwdcolor="#f4ff38"
                     /ApEinfo_revcolor="#f4ff38"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    1623..1642
                     /locus_tag="pcag_fw_seq primer (#7)"
                     /label="pcag_fw_seq primer (#7)"
                     /ApEinfo_label="pcag_fw_seq primer (#7)"
                     /ApEinfo_fwdcolor="#faff28"
                     /ApEinfo_revcolor="#faff28"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    4003..4323
                     /locus_tag="cytoplasmic"
                     /label="cytoplasmic"
                     /ApEinfo_label="cytoplasmic"
                     /ApEinfo_fwdcolor="#5cff31"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    complement(2078..2097)
                     /locus_tag="Sema4D_N_R1"
                     /label="Sema4D_N_R1"
                     /ApEinfo_label="Sema4D_N_R1"
                     /ApEinfo_fwdcolor="cyan"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    1916..1935
                     /locus_tag="Sema4D_N_F1"
                     /label="Sema4D_N_F1"
                     /ApEinfo_label="Sema4D_N_F1"
                     /ApEinfo_fwdcolor="#fb4ab8"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    2366..2385
                     /locus_tag="Sema4D_N_F2"
                     /label="Sema4D_N_F2"
                     /ApEinfo_label="Sema4D_N_F2"
                     /ApEinfo_fwdcolor="#fb38e6"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    2763..2782
                     /locus_tag="Sema4D_N_F3"
                     /label="Sema4D_N_F3"
                     /ApEinfo_label="Sema4D_N_F3"
                     /ApEinfo_fwdcolor="#fb43e4"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    3160..3179
                     /locus_tag="Sema4D_N_F4"
                     /label="Sema4D_N_F4"
                     /ApEinfo_label="Sema4D_N_F4"
                     /ApEinfo_fwdcolor="#fb10f8"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    3533..3552
                     /locus_tag="Sema4D_N_F5"
                     /label="Sema4D_N_F5"
                     /ApEinfo_label="Sema4D_N_F5"
                     /ApEinfo_fwdcolor="#fb3cf0"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    3914..3933
                     /locus_tag="Sema4D_N_F6"
                     /label="Sema4D_N_F6"
                     /ApEinfo_label="Sema4D_N_F6"
                     /ApEinfo_fwdcolor="#fb2ee2"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
ORIGIN
        1 GTCGACATTG ATTATTGACT AGTTATTAAT AGTAATCAAT TACGGGGTCA TTAGTTCATA
       61 GCCCATATAT GGAGTTCCGC GTTACATAAC TTACGGTAAA TGGCCCGCCT GGCTGACCGC
      121 CCAACGACCC CCGCCCATTG ACGTCAATAA TGACGTATGT TCCCATAGTA ACGCCAATAG
      181 GGACTTTCCA TTGACGTCAA TGGGTGGACT ATTTACGGTA AACTGCCCAC TTGGCAGTAC
      241 ATCAAGTGTA TCATATGCCA AGTACGCCCC CTATTGACGT CAATGACGGT AAATGGCCCG
      301 CCTGGCATTA TGCCCAGTAC ATGACCTTAT GGGACTTTCC TACTTGGCAG TACATCTACG
      361 TATTAGTCAT CGCTATTACC ATGGGTCGAG GTGAGCCCCA CGTTCTGCTT CACTCTCCCC
      421 ATCTCCCCCC CCTCCCCACC CCCAATTTTG TATTTATTTA TTTTTTAATT ATTTTGTGCA
      481 GCGATGGGGG CGGGGGGGGG GGGGGCGCGC GCCAGGCGGG GCGGGGCGGG GCGAGGGGCG
      541 GGGCGGGGCG AGGCGGAGAG GTGCGGCGGC AGCCAATCAG AGCGGCGCGC TCCGAAAGTT
      601 TCCTTTTATG GCGAGGCGGC GGCGGCGGCG GCCCTATAAA AAGCGAAGCG CGCGGCGGGC
      661 GGGAGTCGCT GCGTTGCCTT CGCCCCGTGC CCCGCTCCGC GCCGCCTCGC GCCGCCCGCC
      721 CCGGCTCTGA CTGACCGCGT TACTCCCACA GGTGAGCGGG CGGGACGGCC CTTCTCCTCC
      781 GGGCTGTAAT TAGCGCTTGG TTTAATGACG GCTCGTTTCT TTTCTGTGGC TGCGTGAAAG
      841 CCTTAAAGGG CTCCGGGAGG GCCCTTTGTG CGGGGGGGAG CGGCTCGGGG GGTGCGTGCG
      901 TGTGTGTGTG CGTGGGGAGC GCCGCGTGCG GCCCGCGCTG CCCGGCGGCT GTGAGCGCTG
      961 CGGGCGCGGC GCGGGGCTTT GTGCGCTCCG CGTGTGCGCG AGGGGAGCGC GGCCGGGGGC
     1021 GGTGCCCCGC GGTGCGGGGG GGCTGCGAGG GGAACAAAGG CTGCGTGCGG GGTGTGTGCG
     1081 TGGGGGGGTG AGCAGGGGGT GTGGGCGCGG CGGTCGGGCT GTAACCCCCC CCTGCACCCC
     1141 CCTCCCCGAG TTGCTGAGCA CGGCCCGGCT TCGGGTGCGG GGCTCCGTGC GGGGCGTGGC
     1201 GCGGGGCTCG CCGTGCCGGG CGGGGGGTGG CGGCAGGTGG GGGTGCCGGG CGGGGCGGGG
     1261 CCGCCTCGGG CCGGGGAGGG CTCGGGGGAG GGGCGCGGCG GCCCCGGAGC GCCGGCGGCT
     1321 GTCGAGGCGC GGCGAGCCGC AGCCATTGCC TTTTATGGTA ATCGTGCGAG AGGGCGCAGG
     1381 GACTTCCTTT GTCCCAAATC TGGCGGAGCC GAAATCTGGG AGGCGCCGCC GCACCCCCTC
     1441 TAGCGGGCGC GGGCGAAGCG GTGCGGCGCC GGCAGGAAGG AAATGGGCGG GGAGGGCCTT
     1501 CGTGCGTCGC CGCGCCGCCG TCCCCTTCTC CATCTCCAGC CTCGGGGCTG CCGCAGGGGG
     1561 ACGGCTGCCT TCGGGGGGGA CGGGGCAGGG CGGGGTTCGG CTTCTGGCGT GTGACCGGCG
     1621 GCTCTAGAGC CTCTGCTAAC CATGTTCATG CCTTCTTCTT TTTCCTACAG CTCCTGGGCA
     1681 ACGTGCTGGT TATTGTGCTG TCTCATCATT TTGGCAAAGA ATTCACCCGG GCTAGCCACC
     1741 ATGAGGATGT GTGCCCCCGT TAGGGGGCTG TTCTTGGCCC TGGTGGTAGT GTTGAGAACC
     1801 GCGGTGGCAT TTGCACCTGT GCCTCGGCTC ACCTGGGAAC ATGGAGAGGT AGGTCTGGTG
     1861 CAGTTTCACA AGCCAGGCAT CTTTAACTAC TCGGCCTTGC TGATGAGTGA GGACAAAGAC
     1921 ACTCTGTATG TAGGCGCCCG GGAAGCAGTC TTTGCAGTGA ATGCGCTGAA CATCTCTGAG
     1981 AAGCAACATG AGGTATATTG GAAGGTCTCT GAAGACAAAA AATCCAAGTG TGCAGAGAAG
     2041 GGGAAATCAA AGCAGACGGA ATGCCTAAAC TACATTCGAG TACTACAGCC ACTAAGCAGC
     2101 ACTTCCCTCT ATGTGTGTGG GACCAATGCG TTCCAGCCCA CCTGTGACCA CCTGAACTTG
     2161 ACATCCTTCA AGTTTCTGGG GAAAAGTGAA GATGGCAAAG GAAGATGCCC CTTCGACCCC
     2221 GCCCACAGCT ACACATCAGT CATGGTTGGG GGCGAGCTCT ACTCTGGGAC GTCCTATAAT
     2281 TTCTTGGGCA GTGAACCCAT CATCTCTCGA AACTCTTCCC ACAGTCCCTT GAGGACGGAG
     2341 TATGCCATCC CGTGGCTGAA CGAGCCTAGC TTCGTCTTTG CTGACGTGAT CCAGAAAAGC
     2401 CCAGATGGTC CGGAGGGTGA AGATGACAAG GTCTACTTCT TTTTTACGGA GGTATCCGTG
     2461 GAGTACGAAT TCGTCTTCAA GTTGATGATC CCGCGAGTTG CCAGGGTGTG CAAGGGCGAC
     2521 CAGGGCGGCC TGCGGACTTT GCAAAAAAAG TGGACCTCCT TCCTAAAGGC CAGGCTGATC
     2581 TGCTCCAAGC CAGACAGTGG CCTGGTCTTC AACATACTTC AGGATGTGTT TGTGCTGAGG
     2641 GCCCCGGGCC TCAAGGAGCC TGTGTTCTAT GCGGTCTTCA CCCCACAGCT GAACAATGTG
     2701 GGTCTGTCAG CGGTGTGCGC CTACACACTG GCCACGGTGG AGGCAGTCTT CTCCCGTGGA
     2761 AAGTACATGC AGAGTGCCAC AGTGGAGCAG TCTCACACCA AGTGGGTGCG CTACAATGGC
     2821 CCAGTGCCCA CTCCCCGACC TGGAGCGTGT ATCGACAGTG AGGCCCGGGC AGCCAACTAC
     2881 ACCAGCTCCT TGAATCTCCC AGACAAAACA CTGCAGTTTG TAAAAGACCA CCCTTTGATG
     2941 GATGACTCAG TGACCCCGAT AGACAACAGA CCCAAGCTGA TCAAAAAAGA TGTAAACTAC
     3001 ACCCAGATAG TGGTAGACAG GACCCAGGCC CTGGATGGGA CTTTCTACGA CGTCATGTTC
     3061 ATCAGCACAG ACCGGGGAGC TCTGCATAAA GCAGTCATCC TCACAAAAGA GGTGCATGTC
     3121 ATCGAGGAGA CCCAACTCTT CCGGGACTCT GAACCGGTCC TAACTCTGCT GCTATCGTCA
     3181 AAGAAGGGGA GGAAGTTTGT CTATGCAGGC TCCAACTCTG GAGTGGTCCA AGCGCCCCTG
     3241 GCATTCTGCG AAAAGCACGG TAGCTGTGAA GACTGTGTGT TAGCACGGGA CCCCTACTGT
     3301 GCCTGGAGCC CAGCCATCAA GGCCTGTGTT ACCCTGCACC AGGAAGAGGC CTCCAGCAGG
     3361 GGCTGGATTC AGGACATGAG CGGTGACACA TCCTCATGCC TGGATAAGAG TAAAGAAAGT
     3421 TTCAACCAGC ATTTTTTCAA GCACGGCGGC ACAGCGGAAC TCAAATGTTT CCAAAAGTCC
     3481 AACCTAGCCC GGGTGGTATG GAAGTTCCAG AATGGCGAGT TGAAGGCCGC AAGTCCCAAG
     3541 TACGGCTTTG TGGGCAGGAA GCACCTGCTC ATCTTCAACC TGTCGGACGG AGACAGCGGC
     3601 GTGTACCAGT GCCTGTCAGA GGAAAGGGTG AGGAATAAAA CGGTCTCCCA GCTGCTGGCC
     3661 AAGCACGTTC TGGAAGTGAA GATGGTACCT CGGACCCCCC CCTCACCTAC CTCAGAGGAT
     3721 GCTCAGACAG AAGGTAGTAA GATCACATCC AAAATGCCGG TTGCATCTAC CCAGGGGTCC
     3781 TCTCCCCCTA CCCCGGCTCT GTGGGCAACC TCCCCCAGAG CCGCCACCCT ACCTCCCAAG
     3841 TCCTCCTCCG GCACATCCTG TGAACCAAAG ATGGTCATCA ACACGGTCCC CCAGCTCCAC
     3901 TCAGAGAAGA CGGTGTATCT CAAGTCCAGT GACAACCGCC TGCTCATGTC TCTCCTCCTC
     3961 TTCATCTTTG TCCTCTTCCT CTGCCTCTTT TCCTACAACT GCTACAAGGG CTACCTGCCC
     4021 GGACAGTGCT TAAAATTCCG CTCAGCCCTG CTGCTTGGAA AGAAAACACC CAAGTCAGAC
     4081 TTCTCTGACC TGGAGCAGAG TGTGAAGGAG ACACTGGTCG AGCCTGGGAG CTTCTCCCAG
     4141 CAGAACGGCG ACCACCCCAA GCCAGCCCTG GATACGGGCT ATGAAACGGA GCAGGACACC
     4201 ATCACCAGCA AAGTCCCCAC GGATCGTGAG GACTCGCAAC GGATCGATGA ACTCTCTGCC
     4261 CGGGACAAAC CGTTTGATGT CAAGTGTGAA CTGAAGTTTG CAGATTCGGA TGCTGACGGG
     4321 GACgattata aagatgatga tgataaaTGA GCGGCCGCAC TCCTCAGGTG CAGGCTGCCT
     4381 ATCAGAAGGT GGTGGCTGGT GTGGCCAATG CCCTGGCTCA CAAATACCAC TGAGATCTTT
     4441 TTCCCTCTGC CAAAAATTAT GGGGACATCA TGAAGCCCCT TGAGCATCTG ACTTCTGGCT
     4501 AATAAAGGAA ATTTATTTTC ATTGCAATAG TGTGTTGGAA TTTTTTGTGT CTCTCACTCG
     4561 GAAGGACATA TGGGAGGGCA AATCATTTAA AACATCAGAA TGAGTATTTG GTTTAGAGTT
     4621 TGGCAACATA TGCCATATGC TGGCTGCCAT GAACAAAGGT GGCTATAAAG AGGTCATCAG
     4681 TATATGAAAC AGCCCCCTGC TGTCCATTCC TTATTCCATA GAAAAGCCTT GACTTGAGGT
     4741 TAGATTTTTT TTATATTTTG TTTTGTGTTA TTTTTTTCTT TAACATCCCT AAAATTTTCC
     4801 TTACATGTTT TACTAGCCAG ATTTTTCCTC CTCTCCTGAC TACTCCCAGT CATAGCTGTC
     4861 CCTCTTCTCT TATGAAGATC CCTCGACCTG CAGCCCAAGC TTGGCGTAAT CATGGTCATA
     4921 GCTGTTTCCT GTGTGAAATT GTTATCCGCT CACAATTCCA CACAACATAC GAGCCGGAAG
     4981 CATAAAGTGT AAAGCCTGGG GTGCCTAATG AGTGAGCTAA CTCACATTAA TTGCGTTGCG
     5041 CTCACTGCCC GCTTTCCAGT CGGGAAACCT GTCGTGCCAG CGGATCCGCA TCTCAATTAG
     5101 TCAGCAACCA TAGTCCCGCC CCTAACTCCG CCCATCCCGC CCCTAACTCC GCCCAGTTCC
     5161 GCCCATTCTC CGCCCCATGG CTGACTAATT TTTTTTATTT ATGCAGAGGC CGAGGCCGCC
     5221 TCGGCCTCTG AGCTATTCCA GAAGTAGTGA GGAGGCTTTT TTGGAGGCCT AGGCTTTTGC
     5281 AAAAAGCTAA CTTGTTTATT GCAGCTTATA ATGGTTACAA ATAAAGCAAT AGCATCACAA
     5341 ATTTCACAAA TAAAGCATTT TTTTCACTGC ATTCTAGTTG TGGTTTGTCC AAACTCATCA
     5401 ATGTATCTTA TCATGTCTGG ATCCGCTGCA TTAATGAATC GGCCAACGCG CGGGGAGAGG
     5461 CGGTTTGCGT ATTGGGCGCT CTTCCGCTTC CTCGCTCACT GACTCGCTGC GCTCGGTCGT
     5521 TCGGCTGCGG CGAGCGGTAT CAGCTCACTC AAAGGCGGTA ATACGGTTAT CCACAGAATC
     5581 AGGGGATAAC GCAGGAAAGA ACATGTGAGC AAAAGGCCAG CAAAAGGCCA GGAACCGTAA
     5641 AAAGGCCGCG TTGCTGGCGT TTTTCCATAG GCTCCGCCCC CCTGACGAGC ATCACAAAAA
     5701 TCGACGCTCA AGTCAGAGGT GGCGAAACCC GACAGGACTA TAAAGATACC AGGCGTTTCC
     5761 CCCTGGAAGC TCCCTCGTGC GCTCTCCTGT TCCGACCCTG CCGCTTACCG GATACCTGTC
     5821 CGCCTTTCTC CCTTCGGGAA GCGTGGCGCT TTCTCAATGC TCACGCTGTA GGTATCTCAG
     5881 TTCGGTGTAG GTCGTTCGCT CCAAGCTGGG CTGTGTGCAC GAACCCCCCG TTCAGCCCGA
     5941 CCGCTGCGCC TTATCCGGTA ACTATCGTCT TGAGTCCAAC CCGGTAAGAC ACGACTTATC
     6001 GCCACTGGCA GCAGCCACTG GTAACAGGAT TAGCAGAGCG AGGTATGTAG GCGGTGCTAC
     6061 AGAGTTCTTG AAGTGGTGGC CTAACTACGG CTACACTAGA AGGACAGTAT TTGGTATCTG
     6121 CGCTCTGCTG AAGCCAGTTA CCTTCGGAAA AAGAGTTGGT AGCTCTTGAT CCGGCAAACA
     6181 AACCACCGCT GGTAGCGGTG GTTTTTTTGT TTGCAAGCAG CAGATTACGC GCAGAAAAAA
     6241 AGGATCTCAA GAAGATCCTT TGATCTTTTC TACGGGGTCT GACGCTCAGT GGAACGAAAA
     6301 CTCACGTTAA GGGATTTTGG TCATGAGATT ATCAAAAAGG ATCTTCACCT AGATCCTTTT
     6361 AAATTAAAAA TGAAGTTTTA AATCAATCTA AAGTATATAT GAGTAAACTT GGTCTGACAG
     6421 TTACCAATGC TTAATCAGTG AGGCACCTAT CTCAGCGATC TGTCTATTTC GTTCATCCAT
     6481 AGTTGCCTGA CTCCCCGTCG TGTAGATAAC TACGATACGG GAGGGCTTAC CATCTGGCCC
     6541 CAGTGCTGCA ATGATACCGC GAGACCCACG CTCACCGGCT CCAGATTTAT CAGCAATAAA
     6601 CCAGCCAGCC GGAAGGGCCG AGCGCAGAAG TGGTCCTGCA ACTTTATCCG CCTCCATCCA
     6661 GTCTATTAAT TGTTGCCGGG AAGCTAGAGT AAGTAGTTCG CCAGTTAATA GTTTGCGCAA
     6721 CGTTGTTGCC ATTGCTACAG GCATCGTGGT GTCACGCTCG TCGTTTGGTA TGGCTTCATT
     6781 CAGCTCCGGT TCCCAACGAT CAAGGCGAGT TACATGATCC CCCATGTTGT GCAAAAAAGC
     6841 GGTTAGCTCC TTCGGTCCTC CGATCGTTGT CAGAAGTAAG TTGGCCGCAG TGTTATCACT
     6901 CATGGTTATG GCAGCACTGC ATAATTCTCT TACTGTCATG CCATCCGTAA GATGCTTTTC
     6961 TGTGACTGGT GAGTACTCAA CCAAGTCATT CTGAGAATAG TGTATGCGGC GACCGAGTTG
     7021 CTCTTGCCCG GCGTCAATAC GGGATAATAC CGCGCCACAT AGCAGAACTT TAAAAGTGCT
     7081 CATCATTGGA AAACGTTCTT CGGGGCGAAA ACTCTCAAGG ATCTTACCGC TGTTGAGATC
     7141 CAGTTCGATG TAACCCACTC GTGCACCCAA CTGATCTTCA GCATCTTTTA CTTTCACCAG
     7201 CGTTTCTGGG TGAGCAAAAA CAGGAAGGCA AAATGCCGCA AAAAAGGGAA TAAGGGCGAC
     7261 ACGGAAATGT TGAATACTCA TACTCTTCCT TTTTCAATAT TATTGAAGCA TTTATCAGGG
     7321 TTATTGTCTC ATGAGCGGAT ACATATTTGA ATGTATTTAG AAAAATAAAC AAATAGGGGT
     7381 TCCGCGCACA TTTCCCCGAA AAGTGCCACC TG  
//
