LOCUS       Exported                7866 bp DNA     circular SYN 10-AUG-2022
DEFINITION  .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7866)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Aug 10, 2022 from SnapGene Viewer 6.0.5
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..7866
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    1..1622
                     /locus_tag="CAG"
                     /label=CAG
                     /note="color: #ff5101"
     CDS             1731..3467
                     /label=Sema7A Delta-IG
                     /note="color: #ccffcc; direction: RIGHT"
     misc_feature    1731..1868
                     /locus_tag="signal peptide"
                     /label=signal peptide
                     /note="color: #ff4bb6"
     misc_feature    1890..3191
                     /locus_tag="SEMA DOMAIN"
                     /label=SEMA DOMAIN
                     /note="color: #00ffff"
     misc_feature    3198..3350
                     /locus_tag="PSI DOMAIN"
                     /label=PSI DOMAIN
                     /note="color: #ffb65f"
     misc_feature    3402..3458
                     /locus_tag="TRANSMEMBRANE"
                     /label=TRANSMEMBRANE
                     /note="color: #efff8e"
     misc_feature    3465..3467
                     /locus_tag="STOP"
                     /label=STOP
                     /note="color: #ff283a"
     misc_feature    3509..4080
                     /locus_tag="IRES"
                     /label=IRES
                     /note="color: #fffa71"
     misc_feature    4086..4804
                     /locus_tag="EGFP"
                     /label=EGFP
                     /note="color: #20ff3c"
ORIGIN
        1 gtcgacattg attattgact agttattaat agtaatcaat tacggggtca ttagttcata
       61 gcccatatat ggagttccgc gttacataac ttacggtaaa tggcccgcct ggctgaccgc
      121 ccaacgaccc ccgcccattg acgtcaataa tgacgtatgt tcccatagta acgccaatag
      181 ggactttcca ttgacgtcaa tgggtggact atttacggta aactgcccac ttggcagtac
      241 atcaagtgta tcatatgcca agtacgcccc ctattgacgt caatgacggt aaatggcccg
      301 cctggcatta tgcccagtac atgaccttat gggactttcc tacttggcag tacatctacg
      361 tattagtcat cgctattacc atgggtcgag gtgagcccca cgttctgctt cactctcccc
      421 atctcccccc cctccccacc cccaattttg tatttattta ttttttaatt attttgtgca
      481 gcgatggggg cggggggggg gggggcgcgc gccaggcggg gcggggcggg gcgaggggcg
      541 gggcggggcg aggcggagag gtgcggcggc agccaatcag agcggcgcgc tccgaaagtt
      601 tccttttatg gcgaggcggc ggcggcggcg gccctataaa aagcgaagcg cgcggcgggc
      661 gggagtcgct gcgttgcctt cgccccgtgc cccgctccgc gccgcctcgc gccgcccgcc
      721 ccggctctga ctgaccgcgt tactcccaca ggtgagcggg cgggacggcc cttctcctcc
      781 gggctgtaat tagcgcttgg tttaatgacg gctcgtttct tttctgtggc tgcgtgaaag
      841 ccttaaaggg ctccgggagg gccctttgtg cgggggggag cggctcgggg ggtgcgtgcg
      901 tgtgtgtgtg cgtggggagc gccgcgtgcg gcccgcgctg cccggcggct gtgagcgctg
      961 cgggcgcggc gcggggcttt gtgcgctccg cgtgtgcgcg aggggagcgc ggccgggggc
     1021 ggtgccccgc ggtgcggggg ggctgcgagg ggaacaaagg ctgcgtgcgg ggtgtgtgcg
     1081 tgggggggtg agcagggggt gtgggcgcgg cggtcgggct gtaacccccc cctgcacccc
     1141 cctccccgag ttgctgagca cggcccggct tcgggtgcgg ggctccgtgc ggggcgtggc
     1201 gcggggctcg ccgtgccggg cggggggtgg cggcaggtgg gggtgccggg cggggcgggg
     1261 ccgcctcggg ccggggaggg ctcgggggag gggcgcggcg gccccggagc gccggcggct
     1321 gtcgaggcgc ggcgagccgc agccattgcc ttttatggta atcgtgcgag agggcgcagg
     1381 gacttccttt gtcccaaatc tggcggagcc gaaatctggg aggcgccgcc gcaccccctc
     1441 tagcgggcgc gggcgaagcg gtgcggcgcc ggcaggaagg aaatgggcgg ggagggcctt
     1501 cgtgcgtcgc cgcgccgccg tccccttctc catctccagc ctcggggctg ccgcaggggg
     1561 acggctgcct tcggggggga cggggcaggg cggggttcgg cttctggcgt gtgaccggcg
     1621 gctctagagc ctctgctaac catgttcatg ccttcttctt tttcctacag ctcctgggca
     1681 acgtgctggt tgttgtgctg tctcatcatt ttggcaaaga attctgcggg atgacgcctc
     1741 ctcctcccgg acgtgccgcc cccagcgcac cgcgcgcccg cgtcctcagc ctgccggctc
     1801 ggttcgggct cccgctgcgg ctgcggcttc tgctggtgtt ctgggtggcc gccgcctccg
     1861 cccaaggcca ctcgaggagc ggaccccgca tctccgccgt ctggaaaggg caggaccatg
     1921 tggactttag ccagcctgag ccacacaccg tgcttttcca tgagccgggc agcttctctg
     1981 tctgggtggg tggacgtggc aaggtctacc acttcaactt ccccgagggc aagaatgcct
     2041 ctgtgcgcac ggtgaacatc ggctccacaa aggggtcctg tcaggacaaa caggactgtg
     2101 ggaattacat cactcttcta gaaaggcggg gtaatgggct gctggtctgt ggcaccaatg
     2161 cccggaagcc cagctgctgg aacttggtga atgacagtgt ggtgatgtca cttggtgaga
     2221 tgaaaggcta tgcccccttc agcccggatg agaactccct ggttctgttt gaaggagatg
     2281 aagtgtactc taccatccgg aagcaggaat acaacgggaa gatccctcgg tttcgacgca
     2341 ttcggggcga gagtgaactg tacacaagtg atacagtcat gcagaaccca cagttcatca
     2401 aggccaccat tgtgcaccaa gaccaagcct atgatgataa gatctactac ttcttccgag
     2461 aagacaaccc tgacaagaac cccgaggctc ctctcaatgt gtcccgagta gcccagttgt
     2521 gcagggggga ccagggtggt gagagttcgt tgtctgtctc caagtggaac accttcctga
     2581 aagccatgtt ggtctgcagc gatgcagcca ccaacaggaa cttcaatcgg ctgcaagatg
     2641 tcttcctgct ccctgacccc agtggccagt ggagagatac cagggtctat ggcgttttct
     2701 ccaacccctg gaactactca gctgtctgcg tgtattcgct tggtgacatt gacagagtct
     2761 tccgtacctc atcgctcaaa ggctaccaca tgggccttcc caaccctcga cctggcatgt
     2821 gcctcccaaa aaagcagccc atacccacag aaaccttcca ggtagctgat agtcacccag
     2881 aggtggctca gagggtggaa cctatggggc cactgaagac accattgttc cattctaagt
     2941 accattacca gaaagtggtc gttcaccgca tgcaagccag caatggagag accttccatg
     3001 tgctttatct aaccacagac aggggcacca ttcacaaggt ggtggaatca ggggaccagg
     3061 accatagctt tgtcttcaat atcatggaga tccagccctt tcaccgtgca gcggccatcc
     3121 aggctatatc attggatgct gaccggcgga agctctatgt gacctcccag tgggaagtga
     3181 gccaggtacc cctggacatg tgtgaggtct acagcggggg ctgccatggc tgcctcatgt
     3241 cccgagaccc ctactgtggc tgggaccagg accgctgcgt gtctatctac agctcccaac
     3301 gatcagtgct gcagtccatt aatccagcgg agccacacag agagtgtccc aacctgccag
     3361 aggacagagc actggccgaa caactaatgg gccatgcccg ggccctggcc gcctccttct
     3421 ggctgggggt cctgcccaca ctcatacttg gtctgctggt tcactaggcg gccgctacgt
     3481 aaattccgcc cccccccccc ctctccctcc ccccccccta acgttactgg ccgaagccgc
     3541 ttggaataag gccggtgtgc gtttgtctat atgttatttt ccaccatatt gccgtctttt
     3601 ggcaatgtga gggcccggaa acctggccct gtcttcttga cgagcattcc taggggtctt
     3661 tcccctctcg ccaaaggaat gcaaggtctg ttgaatgtcg tgaaggaagc agttcctctg
     3721 gaagcttctt gaagacaaac aacgtctgta gcgacccttt gcaggcagcg gaacccccca
     3781 cctggcgaca ggtgcctctg cggccaaaag ccacgtgtat aagatacacc tgcaaaggcg
     3841 gcacaacccc agtgccacgt tgtgagttgg atagttgtgg aaagagtcaa atggctctcc
     3901 tcaagcgtat tcaacaaggg gctgaaggat gcccagaagg taccccattg tatgggatct
     3961 gatctggggc ctcggtgcac atgctttaca tgtgtttagt cgaggttaaa aaaacgtcta
     4021 ggccccccga accacgggga cgtggttttc ctttgaaaaa cacgatgata atatggccac
     4081 aaccatggtg agcaagggcg aggagctgtt caccggggtg gtgcccatcc tggtcgagct
     4141 ggacggcgac gtaaacggcc acaagttcag cgtgtccggc gagggcgagg gcgatgccac
     4201 ctacggcaag ctgaccctga agttcatctg caccaccggc aagctgcccg tgccctggcc
     4261 caccctcgtg accaccctga cctacggcgt gcagtgcttc agccgctacc ccgaccacat
     4321 gaagcagcac gacttcttca agtccgccat gcccgaaggc tacgtccagg agcgcaccat
     4381 cttcttcaag gacgacggca actacaagac ccgcgccgag gtgaagttcg agggcgacac
     4441 cctggtgaac cgcatcgagc tgaagggcat cgacttcaag gaggacggca acatcctggg
     4501 gcacaagctg gagtacaact acaacagcca caacgtctat atcatggccg acaagcagaa
     4561 gaacggcatc aaggtgaact tcaagatccg ccacaacatc gaggacggca gcgtgcagct
     4621 cgccgaccac taccagcaga acacccccat cggcgacggc cccgtgctgc tgcccgacaa
     4681 ccactacctg agcacccagt ccgccctgag caaagacccc aacgagaagc gcgatcacat
     4741 ggtcctgctg gagttcgtga ccgccgccgg gatcactctc ggcatggacg agctgtacaa
     4801 gtaaagcggc cggccgccag cacagtggtc gatcgacctg caggcatgca agcttcagcc
     4861 aatgccctgg ctcacaaata ccactgagat ctttttccct ctgccaaaaa ttatggggac
     4921 atcatgaagc cccttgagca tctgacttct ggctaataaa ggaaatttat tttcattgca
     4981 atagtgtgtt ggaatttttt gtgtctctca ctcggaagga catatgggag ggcaaatcat
     5041 ttaaaacatc agaatgagta tttggtttag agtttggcaa catatgccat atgctggctg
     5101 ccatgaacaa aggtggctat aaagaggtca tcagtatatg aaacagcccc ctgctgtcca
     5161 ttccttattc catagaaaag ccttgacttg aggttagatt ttttttatat tttgttttgt
     5221 gttatttttt tctttaacat ccctaaaatt ttccttacat gttttactag ccagattttt
     5281 cctcctctcc tgactactcc cagtcatagc tgtccctctt ctcttatgaa gatccctcga
     5341 cctgcagccc aagcttggcg taatcatggt catagctgtt tcctgtgtga aattgttatc
     5401 cgctcacaat tccacacaac atacgagccg gaagcataaa gtgtaaagcc tggggtgcct
     5461 aatgagtgag ctaactcaca ttaattgcgt tgcgctcact gcccgctttc cagtcgggaa
     5521 acctgtcgtg ccagcggatc cgcatctcaa ttagtcagca accatagtcc cgcccctaac
     5581 tccgcccatc ccgcccctaa ctccgcccag ttccgcccat tctccgcccc atggctgact
     5641 aatttttttt atttatgcag aggccgaggc cgcctcggcc tctgagctat tccagaagta
     5701 gtgaggaggc ttttttggag gcctaggctt ttgcaaaaag ctaacttgtt tattgcagct
     5761 tataatggtt acaaataaag caatagcatc acaaatttca caaataaagc atttttttca
     5821 ctgcattcta gttgtggttt gtccaaactc atcaatgtat cttatcatgt ctggatccgc
     5881 tgcattaatg aatcggccaa cgcgcgggga gaggcggttt gcgtattggg cgctcttccg
     5941 cttcctcgct cactgactcg ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc
     6001 actcaaaggc ggtaatacgg ttatccacag aatcagggga taacgcagga aagaacatgt
     6061 gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc
     6121 ataggctccg cccccctgac gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa
     6181 acccgacagg actataaaga taccaggcgt ttccccctgg aagctccctc gtgcgctctc
     6241 ctgttccgac cctgccgctt accggatacc tgtccgcctt tctcccttcg ggaagcgtgg
     6301 cgctttctca atgctcacgc tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc
     6361 tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc ggtaactatc
     6421 gtcttgagtc caacccggta agacacgact tatcgccact ggcagcagcc actggtaaca
     6481 ggattagcag agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg tggcctaact
     6541 acggctacac tagaaggaca gtatttggta tctgcgctct gctgaagcca gttaccttcg
     6601 gaaaaagagt tggtagctct tgatccggca aacaaaccac cgctggtagc ggtggttttt
     6661 ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat cctttgatct
     6721 tttctacggg gtctgacgct cagtggaacg aaaactcacg ttaagggatt ttggtcatga
     6781 gattatcaaa aaggatcttc acctagatcc ttttaaatta aaaatgaagt tttaaatcaa
     6841 tctaaagtat atatgagtaa acttggtctg acagttacca atgcttaatc agtgaggcac
     6901 ctatctcagc gatctgtcta tttcgttcat ccatagttgc ctgactcccc gtcgtgtaga
     6961 taactacgat acgggagggc ttaccatctg gccccagtgc tgcaatgata ccgcgagacc
     7021 cacgctcacc ggctccagat ttatcagcaa taaaccagcc agccggaagg gccgagcgca
     7081 gaagtggtcc tgcaacttta tccgcctcca tccagtctat taattgttgc cgggaagcta
     7141 gagtaagtag ttcgccagtt aatagtttgc gcaacgttgt tgccattgct acaggcatcg
     7201 tggtgtcacg ctcgtcgttt ggtatggctt cattcagctc cggttcccaa cgatcaaggc
     7261 gagttacatg atcccccatg ttgtgcaaaa aagcggttag ctccttcggt cctccgatcg
     7321 ttgtcagaag taagttggcc gcagtgttat cactcatggt tatggcagca ctgcataatt
     7381 ctcttactgt catgccatcc gtaagatgct tttctgtgac tggtgagtac tcaaccaagt
     7441 cattctgaga atagtgtatg cggcgaccga gttgctcttg cccggcgtca atacgggata
     7501 ataccgcgcc acatagcaga actttaaaag tgctcatcat tggaaaacgt tcttcggggc
     7561 gaaaactctc aaggatctta ccgctgttga gatccagttc gatgtaaccc actcgtgcac
     7621 ccaactgatc ttcagcatct tttactttca ccagcgtttc tgggtgagca aaaacaggaa
     7681 ggcaaaatgc cgcaaaaaag ggaataaggg cgacacggaa atgttgaata ctcatactct
     7741 tcctttttca atattattga agcatttatc agggttattg tctcatgagc ggatacatat
     7801 ttgaatgtat ttagaaaaat aaacaaatag gggttccgcg cacatttccc cgaaaagtgc
     7861 cacctg
//
