LOCUS       Exported                7971 bp DNA     circular SYN 10-AUG-2022
DEFINITION  .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7971)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Aug 10, 2022 from SnapGene Viewer 6.0.5
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..7971
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    1..1622
                     /locus_tag="CAG"
                     /label=CAG
                     /note="color: #ff5101"
     CDS             1731..3572
                     /label=Sema7A-Delta-PSI
                     /note="color: #ccffcc; direction: RIGHT"
     misc_feature    1731..1868
                     /locus_tag="Signal Peptide"
                     /label=Signal Peptide
                     /note="color: #ff49a4"
     misc_feature    1890..3191
                     /locus_tag="SEMA DOMAIN"
                     /label=SEMA DOMAIN
                     /note="color: #993366"
     misc_feature    3201..3458
                     /locus_tag="IG-LIKE"
                     /label=IG-LIKE
                     /note="color: #58ff69"
     misc_feature    3507..3563
                     /locus_tag="Transmembrane"
                     /label=Transmembrane
                     /note="color: #f8ff4d"
     misc_feature    3570..3572
                     /locus_tag="STOP"
                     /label=STOP
                     /note="color: #ff2c25"
     misc_feature    3614..4185
                     /locus_tag="IRES"
                     /label=IRES
                     /note="color: #fffa71"
     misc_feature    4191..4909
                     /locus_tag="EGFP"
                     /label=EGFP
                     /note="color: #20ff3c"
ORIGIN
        1 gtcgacattg attattgact agttattaat agtaatcaat tacggggtca ttagttcata
       61 gcccatatat ggagttccgc gttacataac ttacggtaaa tggcccgcct ggctgaccgc
      121 ccaacgaccc ccgcccattg acgtcaataa tgacgtatgt tcccatagta acgccaatag
      181 ggactttcca ttgacgtcaa tgggtggact atttacggta aactgcccac ttggcagtac
      241 atcaagtgta tcatatgcca agtacgcccc ctattgacgt caatgacggt aaatggcccg
      301 cctggcatta tgcccagtac atgaccttat gggactttcc tacttggcag tacatctacg
      361 tattagtcat cgctattacc atgggtcgag gtgagcccca cgttctgctt cactctcccc
      421 atctcccccc cctccccacc cccaattttg tatttattta ttttttaatt attttgtgca
      481 gcgatggggg cggggggggg gggggcgcgc gccaggcggg gcggggcggg gcgaggggcg
      541 gggcggggcg aggcggagag gtgcggcggc agccaatcag agcggcgcgc tccgaaagtt
      601 tccttttatg gcgaggcggc ggcggcggcg gccctataaa aagcgaagcg cgcggcgggc
      661 gggagtcgct gcgttgcctt cgccccgtgc cccgctccgc gccgcctcgc gccgcccgcc
      721 ccggctctga ctgaccgcgt tactcccaca ggtgagcggg cgggacggcc cttctcctcc
      781 gggctgtaat tagcgcttgg tttaatgacg gctcgtttct tttctgtggc tgcgtgaaag
      841 ccttaaaggg ctccgggagg gccctttgtg cgggggggag cggctcgggg ggtgcgtgcg
      901 tgtgtgtgtg cgtggggagc gccgcgtgcg gcccgcgctg cccggcggct gtgagcgctg
      961 cgggcgcggc gcggggcttt gtgcgctccg cgtgtgcgcg aggggagcgc ggccgggggc
     1021 ggtgccccgc ggtgcggggg ggctgcgagg ggaacaaagg ctgcgtgcgg ggtgtgtgcg
     1081 tgggggggtg agcagggggt gtgggcgcgg cggtcgggct gtaacccccc cctgcacccc
     1141 cctccccgag ttgctgagca cggcccggct tcgggtgcgg ggctccgtgc ggggcgtggc
     1201 gcggggctcg ccgtgccggg cggggggtgg cggcaggtgg gggtgccggg cggggcgggg
     1261 ccgcctcggg ccggggaggg ctcgggggag gggcgcggcg gccccggagc gccggcggct
     1321 gtcgaggcgc ggcgagccgc agccattgcc ttttatggta atcgtgcgag agggcgcagg
     1381 gacttccttt gtcccaaatc tggcggagcc gaaatctggg aggcgccgcc gcaccccctc
     1441 tagcgggcgc gggcgaagcg gtgcggcgcc ggcaggaagg aaatgggcgg ggagggcctt
     1501 cgtgcgtcgc cgcgccgccg tccccttctc catctccagc ctcggggctg ccgcaggggg
     1561 acggctgcct tcggggggga cggggcaggg cggggttcgg cttctggcgt gtgaccggcg
     1621 gctctagagc ctctgctaac catgttcatg ccttcttctt tttcctacag ctcctgggca
     1681 acgtgctggt tgttgtgctg tctcatcatt ttggcaaaga attctgcggg atgacgcctc
     1741 ctcctcccgg acgtgccgcc cccagcgcac cgcgcgcccg cgtcctcagc ctgccggctc
     1801 ggttcgggct cccgctgcgg ctgcggcttc tgctggtgtt ctgggtggcc gccgcctccg
     1861 cccaaggcca ctcgaggagc ggaccccgca tctccgccgt ctggaaaggg caggaccatg
     1921 tggactttag ccagcctgag ccacacaccg tgcttttcca tgagccgggc agcttctctg
     1981 tctgggtggg tggacgtggc aaggtctacc acttcaactt ccccgagggc aagaatgcct
     2041 ctgtgcgcac ggtgaacatc ggctccacaa aggggtcctg tcaggacaaa caggactgtg
     2101 ggaattacat cactcttcta gaaaggcggg gtaatgggct gctggtctgt ggcaccaatg
     2161 cccggaagcc cagctgctgg aacttggtga atgacagtgt ggtgatgtca cttggtgaga
     2221 tgaaaggcta tgcccccttc agcccggatg agaactccct ggttctgttt gaaggagatg
     2281 aagtgtactc taccatccgg aagcaggaat acaacgggaa gatccctcgg tttcgacgca
     2341 ttcggggcga gagtgaactg tacacaagtg atacagtcat gcagaaccca cagttcatca
     2401 aggccaccat tgtgcaccaa gaccaagcct atgatgataa gatctactac ttcttccgag
     2461 aagacaaccc tgacaagaac cccgaggctc ctctcaatgt gtcccgagta gcccagttgt
     2521 gcagggggga ccagggtggt gagagttcgt tgtctgtctc caagtggaac accttcctga
     2581 aagccatgtt ggtctgcagc gatgcagcca ccaacaggaa cttcaatcgg ctgcaagatg
     2641 tcttcctgct ccctgacccc agtggccagt ggagagatac cagggtctat ggcgttttct
     2701 ccaacccctg gaactactca gctgtctgcg tgtattcgct tggtgacatt gacagagtct
     2761 tccgtacctc atcgctcaaa ggctaccaca tgggccttcc caaccctcga cctggcatgt
     2821 gcctcccaaa aaagcagccc atacccacag aaaccttcca ggtagctgat agtcacccag
     2881 aggtggctca gagggtggaa cctatggggc cactgaagac accattgttc cattctaagt
     2941 accattacca gaaagtggtc gttcaccgca tgcaagccag caatggagag accttccatg
     3001 tgctttatct aaccacagac aggggcacca ttcacaaggt ggtggaatca ggggaccagg
     3061 accatagctt tgtcttcaat atcatggaga tccagccctt tcaccgtgca gcggccatcc
     3121 aggctatatc attggatgct gaccggcgga agctctatgt gacctcccag tgggaagtga
     3181 gccaggtacc cctggacaac cctaaaccag acgaggctcc attgcagaag gtttccctgg
     3241 ctcggaactc tcgatactac ctgacctgcc ccatggagtc ccgccacgcc acttacttat
     3301 ggcgccatga ggagaatgtg gaacagagct gtgagccagg ccaccaaagc cctagctgca
     3361 tcctgttcat tgagaacctc acggcccgtc agtatggcca ctaccgctgc gaggcccaag
     3421 agggctccta cctccgtgag gctcaacact gggagctgct gccagaggac agagcactgg
     3481 ccgaacaact aatgggccat gcccgggccc tggccgcctc cttctggctg ggggtcctgc
     3541 ccacactcat acttggtctg ctggttcact aggcggccgc tacgtaaatt ccgccccccc
     3601 cccccctctc cctccccccc ccctaacgtt actggccgaa gccgcttgga ataaggccgg
     3661 tgtgcgtttg tctatatgtt attttccacc atattgccgt cttttggcaa tgtgagggcc
     3721 cggaaacctg gccctgtctt cttgacgagc attcctaggg gtctttcccc tctcgccaaa
     3781 ggaatgcaag gtctgttgaa tgtcgtgaag gaagcagttc ctctggaagc ttcttgaaga
     3841 caaacaacgt ctgtagcgac cctttgcagg cagcggaacc ccccacctgg cgacaggtgc
     3901 ctctgcggcc aaaagccacg tgtataagat acacctgcaa aggcggcaca accccagtgc
     3961 cacgttgtga gttggatagt tgtggaaaga gtcaaatggc tctcctcaag cgtattcaac
     4021 aaggggctga aggatgccca gaaggtaccc cattgtatgg gatctgatct ggggcctcgg
     4081 tgcacatgct ttacatgtgt ttagtcgagg ttaaaaaaac gtctaggccc cccgaaccac
     4141 ggggacgtgg ttttcctttg aaaaacacga tgataatatg gccacaacca tggtgagcaa
     4201 gggcgaggag ctgttcaccg gggtggtgcc catcctggtc gagctggacg gcgacgtaaa
     4261 cggccacaag ttcagcgtgt ccggcgaggg cgagggcgat gccacctacg gcaagctgac
     4321 cctgaagttc atctgcacca ccggcaagct gcccgtgccc tggcccaccc tcgtgaccac
     4381 cctgacctac ggcgtgcagt gcttcagccg ctaccccgac cacatgaagc agcacgactt
     4441 cttcaagtcc gccatgcccg aaggctacgt ccaggagcgc accatcttct tcaaggacga
     4501 cggcaactac aagacccgcg ccgaggtgaa gttcgagggc gacaccctgg tgaaccgcat
     4561 cgagctgaag ggcatcgact tcaaggagga cggcaacatc ctggggcaca agctggagta
     4621 caactacaac agccacaacg tctatatcat ggccgacaag cagaagaacg gcatcaaggt
     4681 gaacttcaag atccgccaca acatcgagga cggcagcgtg cagctcgccg accactacca
     4741 gcagaacacc cccatcggcg acggccccgt gctgctgccc gacaaccact acctgagcac
     4801 ccagtccgcc ctgagcaaag accccaacga gaagcgcgat cacatggtcc tgctggagtt
     4861 cgtgaccgcc gccgggatca ctctcggcat ggacgagctg tacaagtaaa gcggccggcc
     4921 gccagcacag tggtcgatcg acctgcaggc atgcaagctt cagccaatgc cctggctcac
     4981 aaataccact gagatctttt tccctctgcc aaaaattatg gggacatcat gaagcccctt
     5041 gagcatctga cttctggcta ataaaggaaa tttattttca ttgcaatagt gtgttggaat
     5101 tttttgtgtc tctcactcgg aaggacatat gggagggcaa atcatttaaa acatcagaat
     5161 gagtatttgg tttagagttt ggcaacatat gccatatgct ggctgccatg aacaaaggtg
     5221 gctataaaga ggtcatcagt atatgaaaca gccccctgct gtccattcct tattccatag
     5281 aaaagccttg acttgaggtt agattttttt tatattttgt tttgtgttat ttttttcttt
     5341 aacatcccta aaattttcct tacatgtttt actagccaga tttttcctcc tctcctgact
     5401 actcccagtc atagctgtcc ctcttctctt atgaagatcc ctcgacctgc agcccaagct
     5461 tggcgtaatc atggtcatag ctgtttcctg tgtgaaattg ttatccgctc acaattccac
     5521 acaacatacg agccggaagc ataaagtgta aagcctgggg tgcctaatga gtgagctaac
     5581 tcacattaat tgcgttgcgc tcactgcccg ctttccagtc gggaaacctg tcgtgccagc
     5641 ggatccgcat ctcaattagt cagcaaccat agtcccgccc ctaactccgc ccatcccgcc
     5701 cctaactccg cccagttccg cccattctcc gccccatggc tgactaattt tttttattta
     5761 tgcagaggcc gaggccgcct cggcctctga gctattccag aagtagtgag gaggcttttt
     5821 tggaggccta ggcttttgca aaaagctaac ttgtttattg cagcttataa tggttacaaa
     5881 taaagcaata gcatcacaaa tttcacaaat aaagcatttt tttcactgca ttctagttgt
     5941 ggtttgtcca aactcatcaa tgtatcttat catgtctgga tccgctgcat taatgaatcg
     6001 gccaacgcgc ggggagaggc ggtttgcgta ttgggcgctc ttccgcttcc tcgctcactg
     6061 actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc agctcactca aaggcggtaa
     6121 tacggttatc cacagaatca ggggataacg caggaaagaa catgtgagca aaaggccagc
     6181 aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg ctccgccccc
     6241 ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg acaggactat
     6301 aaagatacca ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc
     6361 cgcttaccgg atacctgtcc gcctttctcc cttcgggaag cgtggcgctt tctcaatgct
     6421 cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg
     6481 aaccccccgt tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc
     6541 cggtaagaca cgacttatcg ccactggcag cagccactgg taacaggatt agcagagcga
     6601 ggtatgtagg cggtgctaca gagttcttga agtggtggcc taactacggc tacactagaa
     6661 ggacagtatt tggtatctgc gctctgctga agccagttac cttcggaaaa agagttggta
     6721 gctcttgatc cggcaaacaa accaccgctg gtagcggtgg tttttttgtt tgcaagcagc
     6781 agattacgcg cagaaaaaaa ggatctcaag aagatccttt gatcttttct acggggtctg
     6841 acgctcagtg gaacgaaaac tcacgttaag ggattttggt catgagatta tcaaaaagga
     6901 tcttcaccta gatcctttta aattaaaaat gaagttttaa atcaatctaa agtatatatg
     6961 agtaaacttg gtctgacagt taccaatgct taatcagtga ggcacctatc tcagcgatct
     7021 gtctatttcg ttcatccata gttgcctgac tccccgtcgt gtagataact acgatacggg
     7081 agggcttacc atctggcccc agtgctgcaa tgataccgcg agacccacgc tcaccggctc
     7141 cagatttatc agcaataaac cagccagccg gaagggccga gcgcagaagt ggtcctgcaa
     7201 ctttatccgc ctccatccag tctattaatt gttgccggga agctagagta agtagttcgc
     7261 cagttaatag tttgcgcaac gttgttgcca ttgctacagg catcgtggtg tcacgctcgt
     7321 cgtttggtat ggcttcattc agctccggtt cccaacgatc aaggcgagtt acatgatccc
     7381 ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc agaagtaagt
     7441 tggccgcagt gttatcactc atggttatgg cagcactgca taattctctt actgtcatgc
     7501 catccgtaag atgcttttct gtgactggtg agtactcaac caagtcattc tgagaatagt
     7561 gtatgcggcg accgagttgc tcttgcccgg cgtcaatacg ggataatacc gcgccacata
     7621 gcagaacttt aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa ctctcaagga
     7681 tcttaccgct gttgagatcc agttcgatgt aacccactcg tgcacccaac tgatcttcag
     7741 catcttttac tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa
     7801 aaaagggaat aagggcgaca cggaaatgtt gaatactcat actcttcctt tttcaatatt
     7861 attgaagcat ttatcagggt tattgtctca tgagcggata catatttgaa tgtatttaga
     7921 aaaataaaca aataggggtt ccgcgcacat ttccccgaaa agtgccacct g
//
