LOCUS       Exported                8124 bp DNA     circular SYN 10-AUG-2022
DEFINITION  .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8124)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Aug 10, 2022 from SnapGene Viewer 6.0.5
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..8124
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    1..1622
                     /locus_tag="CAG"
                     /label=CAG
                     /note="color: #ff5101"
     CDS             1731..3725
                     /label=Sema7A-KCE
                     /note="color: #ccffcc; direction: RIGHT"
     misc_feature    1731..1868
                     /locus_tag="signal peptide"
                     /label=signal peptide
                     /note="color: #ff8294"
     misc_feature    1890..3191
                     /label=Sema Domain
                     /note="color: #993366"
     misc_feature    2523..2531
                     /locus_tag="KCE mutation"
                     /label=KCE mutation
                     /note="color: #ff9e1c"
     misc_feature    3198..3350
                     /label=PSI Domain
                     /note="color: #ff0000"
     misc_feature    3354..3611
                     /label=IG-like Domain
                     /note="color: #ffff99"
     misc_feature    3660..3716
                     /label=Transmembrane Domain
                     /note="color: #ff9900"
     misc_feature    3767..4338
                     /locus_tag="IRES"
                     /label=IRES
                     /note="color: #fffa71"
     misc_feature    4344..5062
                     /locus_tag="EGFP"
                     /label=EGFP
                     /note="color: #20ff3c"
ORIGIN
        1 gtcgacattg attattgact agttattaat agtaatcaat tacggggtca ttagttcata
       61 gcccatatat ggagttccgc gttacataac ttacggtaaa tggcccgcct ggctgaccgc
      121 ccaacgaccc ccgcccattg acgtcaataa tgacgtatgt tcccatagta acgccaatag
      181 ggactttcca ttgacgtcaa tgggtggact atttacggta aactgcccac ttggcagtac
      241 atcaagtgta tcatatgcca agtacgcccc ctattgacgt caatgacggt aaatggcccg
      301 cctggcatta tgcccagtac atgaccttat gggactttcc tacttggcag tacatctacg
      361 tattagtcat cgctattacc atgggtcgag gtgagcccca cgttctgctt cactctcccc
      421 atctcccccc cctccccacc cccaattttg tatttattta ttttttaatt attttgtgca
      481 gcgatggggg cggggggggg gggggcgcgc gccaggcggg gcggggcggg gcgaggggcg
      541 gggcggggcg aggcggagag gtgcggcggc agccaatcag agcggcgcgc tccgaaagtt
      601 tccttttatg gcgaggcggc ggcggcggcg gccctataaa aagcgaagcg cgcggcgggc
      661 gggagtcgct gcgttgcctt cgccccgtgc cccgctccgc gccgcctcgc gccgcccgcc
      721 ccggctctga ctgaccgcgt tactcccaca ggtgagcggg cgggacggcc cttctcctcc
      781 gggctgtaat tagcgcttgg tttaatgacg gctcgtttct tttctgtggc tgcgtgaaag
      841 ccttaaaggg ctccgggagg gccctttgtg cgggggggag cggctcgggg ggtgcgtgcg
      901 tgtgtgtgtg cgtggggagc gccgcgtgcg gcccgcgctg cccggcggct gtgagcgctg
      961 cgggcgcggc gcggggcttt gtgcgctccg cgtgtgcgcg aggggagcgc ggccgggggc
     1021 ggtgccccgc ggtgcggggg ggctgcgagg ggaacaaagg ctgcgtgcgg ggtgtgtgcg
     1081 tgggggggtg agcagggggt gtgggcgcgg cggtcgggct gtaacccccc cctgcacccc
     1141 cctccccgag ttgctgagca cggcccggct tcgggtgcgg ggctccgtgc ggggcgtggc
     1201 gcggggctcg ccgtgccggg cggggggtgg cggcaggtgg gggtgccggg cggggcgggg
     1261 ccgcctcggg ccggggaggg ctcgggggag gggcgcggcg gccccggagc gccggcggct
     1321 gtcgaggcgc ggcgagccgc agccattgcc ttttatggta atcgtgcgag agggcgcagg
     1381 gacttccttt gtcccaaatc tggcggagcc gaaatctggg aggcgccgcc gcaccccctc
     1441 tagcgggcgc gggcgaagcg gtgcggcgcc ggcaggaagg aaatgggcgg ggagggcctt
     1501 cgtgcgtcgc cgcgccgccg tccccttctc catctccagc ctcggggctg ccgcaggggg
     1561 acggctgcct tcggggggga cggggcaggg cggggttcgg cttctggcgt gtgaccggcg
     1621 gctctagagc ctctgctaac catgttcatg ccttcttctt tttcctacag ctcctgggca
     1681 acgtgctggt tgttgtgctg tctcatcatt ttggcaaaga attctgcggg atgacgcctc
     1741 ctcctcccgg acgtgccgcc cccagcgcac cgcgcgcccg cgtcctcagc ctgccggctc
     1801 ggttcgggct cccgctgcgg ctgcggcttc tgctggtgtt ctgggtggcc gccgcctccg
     1861 cccaaggcca ctcgaggagc ggaccccgca tctccgccgt ctggaaaggg caggaccatg
     1921 tggactttag ccagcctgag ccacacaccg tgcttttcca tgagccgggc agcttctctg
     1981 tctgggtggg tggacgtggc aaggtctacc acttcaactt ccccgagggc aagaatgcct
     2041 ctgtgcgcac ggtgaacatc ggctccacaa aggggtcctg tcaggacaaa caggactgtg
     2101 ggaattacat cactcttcta gaaaggcggg gtaatgggct gctggtctgt ggcaccaatg
     2161 cccggaagcc cagctgctgg aacttggtga atgacagtgt ggtgatgtca cttggtgaga
     2221 tgaaaggcta tgcccccttc agcccggatg agaactccct ggttctgttt gaaggagatg
     2281 aagtgtactc taccatccgg aagcaggaat acaacgggaa gatccctcgg tttcgacgca
     2341 ttcggggcga gagtgaactg tacacaagtg atacagtcat gcagaaccca cagttcatca
     2401 aggccaccat tgtgcaccaa gaccaagcct atgatgataa gatctactac ttcttccgag
     2461 aagacaaccc tgacaagaac cccgaggctc ctctcaatgt gtcccgagta gcccagttgt
     2521 gcaagtgcga gcagggtggt gagagttcgt tgtctgtctc caagtggaac accttcctga
     2581 aagccatgtt ggtctgcagc gatgcagcca ccaacaggaa cttcaatcgg ctgcaagatg
     2641 tcttcctgct ccctgacccc agtggccagt ggagagatac cagggtctat ggcgttttct
     2701 ccaacccctg gaactactca gctgtctgcg tgtattcgct tggtgacatt gacagagtct
     2761 tccgtacctc atcgctcaaa ggctaccaca tgggccttcc caaccctcga cctggcatgt
     2821 gcctcccaaa aaagcagccc atacccacag aaaccttcca ggtagctgat agtcacccag
     2881 aggtggctca gagggtggaa cctatggggc cactgaagac accattgttc cattctaagt
     2941 accattacca gaaagtggtc gttcaccgca tgcaagccag caatggagag accttccatg
     3001 tgctttatct aaccacagac aggggcacca ttcacaaggt ggtggaatca ggggaccagg
     3061 accatagctt tgtcttcaat atcatggaga tccagccctt tcaccgtgca gcggccatcc
     3121 aggctatatc attggatgct gaccggcgga agctctatgt gacctcccag tgggaagtga
     3181 gccaggtacc cctggacatg tgtgaggtct acagcggggg ctgccatggc tgcctcatgt
     3241 cccgagaccc ctactgtggc tgggaccagg accgctgcgt gtctatctac agctcccaac
     3301 gatcagtgct gcagtccatt aatccagcgg agccacacag agagtgtccc aaccctaaac
     3361 cagacgaggc tccattgcag aaggtttccc tggctcggaa ctctcgatac tacctgacct
     3421 gccccatgga gtcccgccac gccacttact tatggcgcca tgaggagaat gtggaacaga
     3481 gctgtgagcc aggccaccaa agccctagct gcatcctgtt cattgagaac ctcacggccc
     3541 gtcagtatgg ccactaccgc tgcgaggccc aagagggctc ctacctccgt gaggctcaac
     3601 actgggagct gctgccagag gacagagcac tggccgaaca actaatgggc catgcccggg
     3661 ccctggccgc ctccttctgg ctgggggtcc tgcccacact catacttggt ctgctggttc
     3721 actaggcggc cgctacgtaa attccgcccc ccccccccct ctccctcccc cccccctaac
     3781 gttactggcc gaagccgctt ggaataaggc cggtgtgcgt ttgtctatat gttattttcc
     3841 accatattgc cgtcttttgg caatgtgagg gcccggaaac ctggccctgt cttcttgacg
     3901 agcattccta ggggtctttc ccctctcgcc aaaggaatgc aaggtctgtt gaatgtcgtg
     3961 aaggaagcag ttcctctgga agcttcttga agacaaacaa cgtctgtagc gaccctttgc
     4021 aggcagcgga accccccacc tggcgacagg tgcctctgcg gccaaaagcc acgtgtataa
     4081 gatacacctg caaaggcggc acaaccccag tgccacgttg tgagttggat agttgtggaa
     4141 agagtcaaat ggctctcctc aagcgtattc aacaaggggc tgaaggatgc ccagaaggta
     4201 ccccattgta tgggatctga tctggggcct cggtgcacat gctttacatg tgtttagtcg
     4261 aggttaaaaa aacgtctagg ccccccgaac cacggggacg tggttttcct ttgaaaaaca
     4321 cgatgataat atggccacaa ccatggtgag caagggcgag gagctgttca ccggggtggt
     4381 gcccatcctg gtcgagctgg acggcgacgt aaacggccac aagttcagcg tgtccggcga
     4441 gggcgagggc gatgccacct acggcaagct gaccctgaag ttcatctgca ccaccggcaa
     4501 gctgcccgtg ccctggccca ccctcgtgac caccctgacc tacggcgtgc agtgcttcag
     4561 ccgctacccc gaccacatga agcagcacga cttcttcaag tccgccatgc ccgaaggcta
     4621 cgtccaggag cgcaccatct tcttcaagga cgacggcaac tacaagaccc gcgccgaggt
     4681 gaagttcgag ggcgacaccc tggtgaaccg catcgagctg aagggcatcg acttcaagga
     4741 ggacggcaac atcctggggc acaagctgga gtacaactac aacagccaca acgtctatat
     4801 catggccgac aagcagaaga acggcatcaa ggtgaacttc aagatccgcc acaacatcga
     4861 ggacggcagc gtgcagctcg ccgaccacta ccagcagaac acccccatcg gcgacggccc
     4921 cgtgctgctg cccgacaacc actacctgag cacccagtcc gccctgagca aagaccccaa
     4981 cgagaagcgc gatcacatgg tcctgctgga gttcgtgacc gccgccggga tcactctcgg
     5041 catggacgag ctgtacaagt aaagcggccg gccgccagca cagtggtcga tcgacctgca
     5101 ggcatgcaag cttcagccaa tgccctggct cacaaatacc actgagatct ttttccctct
     5161 gccaaaaatt atggggacat catgaagccc cttgagcatc tgacttctgg ctaataaagg
     5221 aaatttattt tcattgcaat agtgtgttgg aattttttgt gtctctcact cggaaggaca
     5281 tatgggaggg caaatcattt aaaacatcag aatgagtatt tggtttagag tttggcaaca
     5341 tatgccatat gctggctgcc atgaacaaag gtggctataa agaggtcatc agtatatgaa
     5401 acagccccct gctgtccatt ccttattcca tagaaaagcc ttgacttgag gttagatttt
     5461 ttttatattt tgttttgtgt tatttttttc tttaacatcc ctaaaatttt ccttacatgt
     5521 tttactagcc agatttttcc tcctctcctg actactccca gtcatagctg tccctcttct
     5581 cttatgaaga tccctcgacc tgcagcccaa gcttggcgta atcatggtca tagctgtttc
     5641 ctgtgtgaaa ttgttatccg ctcacaattc cacacaacat acgagccgga agcataaagt
     5701 gtaaagcctg gggtgcctaa tgagtgagct aactcacatt aattgcgttg cgctcactgc
     5761 ccgctttcca gtcgggaaac ctgtcgtgcc agcggatccg catctcaatt agtcagcaac
     5821 catagtcccg cccctaactc cgcccatccc gcccctaact ccgcccagtt ccgcccattc
     5881 tccgccccat ggctgactaa ttttttttat ttatgcagag gccgaggccg cctcggcctc
     5941 tgagctattc cagaagtagt gaggaggctt ttttggaggc ctaggctttt gcaaaaagct
     6001 aacttgttta ttgcagctta taatggttac aaataaagca atagcatcac aaatttcaca
     6061 aataaagcat ttttttcact gcattctagt tgtggtttgt ccaaactcat caatgtatct
     6121 tatcatgtct ggatccgctg cattaatgaa tcggccaacg cgcggggaga ggcggtttgc
     6181 gtattgggcg ctcttccgct tcctcgctca ctgactcgct gcgctcggtc gttcggctgc
     6241 ggcgagcggt atcagctcac tcaaaggcgg taatacggtt atccacagaa tcaggggata
     6301 acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg
     6361 cgttgctggc gtttttccat aggctccgcc cccctgacga gcatcacaaa aatcgacgct
     6421 caagtcagag gtggcgaaac ccgacaggac tataaagata ccaggcgttt ccccctggaa
     6481 gctccctcgt gcgctctcct gttccgaccc tgccgcttac cggatacctg tccgcctttc
     6541 tcccttcggg aagcgtggcg ctttctcaat gctcacgctg taggtatctc agttcggtgt
     6601 aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg
     6661 ccttatccgg taactatcgt cttgagtcca acccggtaag acacgactta tcgccactgg
     6721 cagcagccac tggtaacagg attagcagag cgaggtatgt aggcggtgct acagagttct
     6781 tgaagtggtg gcctaactac ggctacacta gaaggacagt atttggtatc tgcgctctgc
     6841 tgaagccagt taccttcgga aaaagagttg gtagctcttg atccggcaaa caaaccaccg
     6901 ctggtagcgg tggttttttt gtttgcaagc agcagattac gcgcagaaaa aaaggatctc
     6961 aagaagatcc tttgatcttt tctacggggt ctgacgctca gtggaacgaa aactcacgtt
     7021 aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa
     7081 aatgaagttt taaatcaatc taaagtatat atgagtaaac ttggtctgac agttaccaat
     7141 gcttaatcag tgaggcacct atctcagcga tctgtctatt tcgttcatcc atagttgcct
     7201 gactccccgt cgtgtagata actacgatac gggagggctt accatctggc cccagtgctg
     7261 caatgatacc gcgagaccca cgctcaccgg ctccagattt atcagcaata aaccagccag
     7321 ccggaagggc cgagcgcaga agtggtcctg caactttatc cgcctccatc cagtctatta
     7381 attgttgccg ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg
     7441 ccattgctac aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg
     7501 gttcccaacg atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa gcggttagct
     7561 ccttcggtcc tccgatcgtt gtcagaagta agttggccgc agtgttatca ctcatggtta
     7621 tggcagcact gcataattct cttactgtca tgccatccgt aagatgcttt tctgtgactg
     7681 gtgagtactc aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc
     7741 cggcgtcaat acgggataat accgcgccac atagcagaac tttaaaagtg ctcatcattg
     7801 gaaaacgttc ttcggggcga aaactctcaa ggatcttacc gctgttgaga tccagttcga
     7861 tgtaacccac tcgtgcaccc aactgatctt cagcatcttt tactttcacc agcgtttctg
     7921 ggtgagcaaa aacaggaagg caaaatgccg caaaaaaggg aataagggcg acacggaaat
     7981 gttgaatact catactcttc ctttttcaat attattgaag catttatcag ggttattgtc
     8041 tcatgagcgg atacatattt gaatgtattt agaaaaataa acaaataggg gttccgcgca
     8101 catttccccg aaaagtgcca cctg
//
