LOCUS       Exported                7413 bp DNA     circular SYN 10-AUG-2022
DEFINITION  .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7413)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Aug 10, 2022 from SnapGene Viewer 6.0.5
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..7413
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    34..356
                     /locus_tag="CMV"
                     /label=CMV
                     /note="color: #00ffff"
     misc_feature    170..189
                     /locus_tag="pCAGkanafwsequ"
                     /label=pCAGkanafwsequ
                     /note="color: #00ffff"
     misc_feature    386..661
                     /locus_tag="CAG enhancer"
                     /label=CAG enhancer
                     /note="color: #1460eb"
     misc_feature    662..1669
                     /locus_tag="rb beta globin stuff"
                     /label=rb beta globin stuff
                     /note="color: #808000"
     misc_feature    1678..1697
                     /locus_tag="CAGfw"
                     /label=CAGfw
                     /note="color: #00ffff"
     CDS             1720..4338
                     /codon_start=1
                     /label=sp-myc-hSema4D-497P
                     /note="color: #ccffcc"
                     /translation="MRMCTPIRGLLMALAVMFGTAMAEQKLISEEDLFAPIPRITWEHR
                     EVHLVQFHEPDIYNYSALLLSEDKDTLYIGAREAVFAVNALNISEKQHEVYWKVSEDKK
                     AKCAEKGKSKQTECLNYIRVLQPLSATSLYVCGTNAFQPACDHLNLTSFKFLGKNEDGK
                     GRCPFDPAHSYTSVMVDGELYSGTSYNFLGSEPIISRNSSHSPLRTEYAIPWLNEPSFV
                     FADVIRKSPDSPDGEDDRVYFFFTEVSVEYEFVFRVLIPRIARVCKGDQGGLRTLQKKW
                     TSFLKARLICSRPDSGLVFNVLRDVFVLRSPGLKVPVFYALFTPQLNNVGLSAVCAYNL
                     STAEEVFSHGKYMQSTTVEQSHTKWVRYNGPVPKPRPGACIDSEARAANYTSSLNLPDK
                     TLQFVKDHPLMDDSVTPIDNRPRLIKKDVNYTQIVVDRTQALDGTVYDVMFVSTDRGAL
                     HKAISLEHAVHIIEETQLFQDFEPVQTLLLSSKKGNRFVYAGSNSGVVPAPLAFCGKHG
                     TCEDCVLARDPYCAWSPPTATCVALHQTESPSRGLIQEMSGDASVCPDKSKGSYRQHFF
                     KHGGTAELKCSQKSNLARVFWKFQNGVLKAESPKYGLMGRKNLLIFNLSEGDSGVYQCL
                     SEERVKNKTVFQVVAKHVLEVKVVPKPVVAPTLSVVQTEGSRIATKVLVASTQGSSPPT
                     PAVQATSSGAITLPPKPAPTGTSCEPKIVINTVPQLHSEKTMYLKSSDNRLLMSLFLFF
                     FVLFLCLFFYNCYKGYLPRQCLKFRSALLIGKKKPKSDFCDREQSLKETLVEPGSFSQQ
                     NGEHPKPALDTGYETEQDTITSKVPTDREDSQRIDDLSARDKPFDVKCELKFADSDADG
                     D"
     misc_feature    1720..1788
                     /locus_tag="Signal-myc-hmSEMA4D"
                     /label=Signal-myc-hmSEMA4D
                     /note="color: #00ffff"
     misc_feature    1789..1818
                     /locus_tag="myc"
                     /label=myc
                     /note="color: #800080"
     misc_feature    3238..3240
                     /label=497P
                     /note="color: #ff6600"
     misc_feature    4517..4539
                     /locus_tag="CAG rev"
                     /label=CAG rev
                     /note="color: #00ffff"
     primer_bind     complement(4912..4932)
                     /locus_tag="M13-rev"
                     /label=M13-rev
                     /note="color: #00ff00; direction: LEFT"
     misc_feature    4916..4932
                     /locus_tag="M13rev"
                     /label=M13rev
                     /note="color: #00ffff"
     misc_binding    complement(4938..4960)
                     /locus_tag="LacO"
                     /label=LacO
                     /note="color: #6495ed; direction: LEFT"
     misc_feature    5378..5398
                     /locus_tag="pCAGkanarevsequ"
                     /label=pCAGkanarevsequ
                     /note="color: #00ffff"
     rep_origin      complement(5645..6327)
                     /direction=LEFT
                     /locus_tag="ColE1 origin"
                     /label=ColE1 origin
                     /note="color: #808080"
     CDS             complement(6425..7084)
                     /locus_tag="AmpR"
                     /label=AmpR
                     /note="color: #ffff00; direction: LEFT"
ORIGIN
        1 gtcgacattg attattgact agttattaat agtaatcaat tacggggtca ttagttcata
       61 gcccatatat ggagttccgc gttacataac ttacggtaaa tggcccgcct ggctgaccgc
      121 ccaacgaccc ccgcccattg acgtcaataa tgacgtatgt tcccatagta acgccaatag
      181 ggactttcca ttgacgtcaa tgggtggact atttacggta aactgcccac ttggcagtac
      241 atcaagtgta tcatatgcca agtacgcccc ctattgacgt caatgacggt aaatggcccg
      301 cctggcatta tgcccagtac atgaccttat gggactttcc tacttggcag tacatctacg
      361 tattagtcat cgctattacc atgggtcgag gtgagcccca cgttctgctt cactctcccc
      421 atctcccccc cctccccacc cccaattttg tatttattta ttttttaatt attttgtgca
      481 gcgatggggg cggggggggg gggggcgcgc gccaggcggg gcggggcggg gcgaggggcg
      541 gggcggggcg aggcggagag gtgcggcggc agccaatcag agcggcgcgc tccgaaagtt
      601 tccttttatg gcgaggcggc ggcggcggcg gccctataaa aagcgaagcg cgcggcgggc
      661 gggagtcgct gcgttgcctt cgccccgtgc cccgctccgc gccgcctcgc gccgcccgcc
      721 ccggctctga ctgaccgcgt tactcccaca ggtgagcggg cgggacggcc cttctcctcc
      781 gggctgtaat tagcgcttgg tttaatgacg gctcgtttct tttctgtggc tgcgtgaaag
      841 ccttaaaggg ctccgggagg gccctttgtg cgggggggag cggctcgggg ggtgcgtgcg
      901 tgtgtgtgtg cgtggggagc gccgcgtgcg gcccgcgctg cccggcggct gtgagcgctg
      961 cgggcgcggc gcggggcttt gtgcgctccg cgtgtgcgcg aggggagcgc ggccgggggc
     1021 ggtgccccgc ggtgcggggg ggctgcgagg ggaacaaagg ctgcgtgcgg ggtgtgtgcg
     1081 tgggggggtg agcagggggt gtgggcgcgg cggtcgggct gtaacccccc cctgcacccc
     1141 cctccccgag ttgctgagca cggcccggct tcgggtgcgg ggctccgtgc ggggcgtggc
     1201 gcggggctcg ccgtgccggg cggggggtgg cggcaggtgg gggtgccggg cggggcgggg
     1261 ccgcctcggg ccggggaggg ctcgggggag gggcgcggcg gccccggagc gccggcggct
     1321 gtcgaggcgc ggcgagccgc agccattgcc ttttatggta atcgtgcgag agggcgcagg
     1381 gacttccttt gtcccaaatc tggcggagcc gaaatctggg aggcgccgcc gcaccccctc
     1441 tagcgggcgc gggcgaagcg gtgcggcgcc ggcaggaagg aaatgggcgg ggagggcctt
     1501 cgtgcgtcgc cgcgccgccg tccccttctc catctccagc ctcggggctg ccgcaggggg
     1561 acggctgcct tcggggggga cggggcaggg cggggttcgg cttctggcgt gtgaccggcg
     1621 gctctagagc ctctgctaac catgttcatg ccttcttctt tttcctacag ctcctgggca
     1681 acgtgctggt tattgtgctg tctcatcatt ttggcaaaga tgaggatgtg cacccccatt
     1741 agggggctgc tcatggccct tgcagtgatg tttgggacag cgatggcaga acaaaaactc
     1801 atctcagaag aggatctgtt tgcacccata ccccggatca cctgggagca cagagaggtg
     1861 cacctggtgc agtttcatga gccagacatc tacaactact cagccttgct gctgagcgag
     1921 gacaaggaca ccttgtacat aggtgcccgg gaggcggtct tcgctgtgaa cgcactcaac
     1981 atctccgaga agcagcatga ggtgtattgg aaggtctcag aagacaaaaa agcaaaatgt
     2041 gcagaaaagg ggaaatcaaa acagacagag tgcctcaact acatccgggt gctgcagcca
     2101 ctcagcgcca cttcccttta cgtgtgtggg accaacgcat tccagccggc ctgtgaccac
     2161 ctgaacttaa catcctttaa gtttctgggg aaaaatgaag atggcaaagg aagatgtccc
     2221 tttgacccag cacacagcta cacatccgtc atggttgatg gagaacttta ttcggggacg
     2281 tcgtataatt ttttgggaag tgaacccatc atctcccgaa attcttccca cagtcctctg
     2341 aggacagaat atgcaatccc ttggctgaac gagcctagtt tcgtgtttgc tgacgtgatc
     2401 cgaaaaagcc cagacagccc cgacggcgag gatgacaggg tctacttctt cttcacggag
     2461 gtgtctgtgg agtatgagtt tgtgttcagg gtgctgatcc cacggatagc aagagtgtgc
     2521 aagggggacc agggcggcct gaggaccttg cagaagaaat ggacctcctt cctgaaagcc
     2581 cgactcatct gctcccggcc agacagcggc ttggtcttca atgtgctgcg ggatgtcttc
     2641 gtgctcaggt ccccgggcct gaaggtgcct gtgttctatg cactcttcac cccacagctg
     2701 aacaacgtgg ggctgtcggc agtgtgcgcc tacaacctgt ccacagccga ggaggtcttc
     2761 tcccacggga agtacatgca gagcaccaca gtggagcagt cccacaccaa gtgggtgcgc
     2821 tataatggcc cggtacccaa gccgcggcct ggagcgtgca tcgacagcga ggcacgggcc
     2881 gccaactaca ccagctcctt gaatttgcca gacaagacgc tgcagttcgt taaagaccac
     2941 cctttgatgg atgactcggt aaccccaata gacaacaggc ccaggttaat caagaaagat
     3001 gtgaactaca cccagatcgt ggtggaccgg acccaggccc tggatgggac tgtctatgat
     3061 gtcatgtttg tcagcacaga ccggggagct ctgcacaaag ccatcagcct cgagcacgct
     3121 gttcacatca tcgaggagac ccagctcttc caggactttg agccagtcca gaccctgctg
     3181 ctgtcttcaa agaagggcaa caggtttgtc tatgctggct ctaactcggg cgtggtcccc
     3241 gccccgctgg ccttctgtgg gaagcacggc acctgcgagg actgtgtgct ggcgcgggac
     3301 ccctactgcg cctggagccc gcccacagcg acctgcgtgg ctctgcacca gaccgagagc
     3361 cccagcaggg gtttgattca ggagatgagc ggcgatgctt ctgtgtgccc ggataaaagt
     3421 aaaggaagtt accggcagca ttttttcaag cacggtggca cagcggaact gaaatgctcc
     3481 caaaaatcca acctggcccg ggtcttttgg aagttccaga atggcgtgtt gaaggccgag
     3541 agccccaagt acggtcttat gggcagaaaa aacttgctca tcttcaactt gtcagaagga
     3601 gacagtgggg tgtaccagtg cctgtcagag gagagggtta agaacaaaac ggtcttccaa
     3661 gtggtcgcca agcacgtcct ggaagtgaag gtggttccaa agcccgtagt ggcccccacc
     3721 ttgtcagttg ttcagacaga aggtagtagg attgccacca aagtgttggt ggcatccacc
     3781 caagggtctt ctcccccaac cccagccgtg caggccacct cctccggggc catcaccctt
     3841 cctcccaagc ctgcgcccac cggcacatcc tgcgaaccaa agatcgtcat caacacggtc
     3901 ccccagctcc actcggagaa aaccatgtat cttaagtcca gcgacaaccg cctcctcatg
     3961 tccctcttcc tcttcttctt tgttctcttc ctctgcctct ttttctacaa ctgctataag
     4021 ggatacctgc ccagacagtg cttgaaattc cgctcggccc tactaattgg gaagaagaag
     4081 cccaagtcag atttctgtga ccgtgagcag agcctgaagg agacgttagt agagccaggg
     4141 agcttctccc agcagaatgg ggagcacccc aagccagccc tggacaccgg ctatgagacc
     4201 gagcaagaca ccatcaccag caaagtcccc acggataggg aggactcaca gaggatcgac
     4261 gacctttctg ccagggacaa gccctttgac gtcaagtgtg agctgaagtt cgctgactca
     4321 gacgcagatg gagactgacc tcgaggatat cgcggccgca ctcctcaggt gcaggctgcc
     4381 tatcagaagg tggtggctgg tgtggccaat gccctggctc acaaatacca ctgagatctt
     4441 tttccctctg ccaaaaatta tggggacatc atgaagcccc ttgagcatct gacttctggc
     4501 taataaagga aatttatttt cattgcaata gtgtgttgga attttttgtg tctctcactc
     4561 ggaaggacat atgggagggc aaatcattta aaacatcaga atgagtattt ggtttagagt
     4621 ttggcaacat atgccatatg ctggctgcca tgaacaaagg tggctataaa gaggtcatca
     4681 gtatatgaaa cagccccctg ctgtccattc cttattccat agaaaagcct tgacttgagg
     4741 ttagattttt tttatatttt gttttgtgtt atttttttct ttaacatccc taaaattttc
     4801 cttacatgtt ttactagcca gatttttcct cctctcctga ctactcccag tcatagctgt
     4861 ccctcttctc ttatgaagat ccctcgacct gcagcccaag cttggcgtaa tcatggtcat
     4921 agctgtttcc tgtgtgaaat tgttatccgc tcacaattcc acacaacata cgagccggaa
     4981 gcataaagtg taaagcctgg ggtgcctaat gagtgagcta actcacatta attgcgttgc
     5041 gctcactgcc cgctttccag tcgggaaacc tgtcgtgcca gcggatccgc atctcaatta
     5101 gtcagcaacc atagtcccgc ccctaactcc gcccatcccg cccctaactc cgcccagttc
     5161 cgcccattct ccgccccatg gctgactaat tttttttatt tatgcagagg ccgaggccgc
     5221 ctcggcctct gagctattcc agaagtagtg aggaggcttt tttggaggcc taggcttttg
     5281 caaaaagcta acttgtttat tgcagcttat aatggttaca aataaagcaa tagcatcaca
     5341 aatttcacaa ataaagcatt tttttcactg cattctagtt gtggtttgtc caaactcatc
     5401 aatgtatctt atcatgtctg gatccgctgc attaatgaat cggccaacgc gcggggagag
     5461 gcggtttgcg tattgggcgc tcttccgctt cctcgctcac tgactcgctg cgctcggtcg
     5521 ttcggctgcg gcgagcggta tcagctcact caaaggcggt aatacggtta tccacagaat
     5581 caggggataa cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta
     5641 aaaaggccgc gttgctggcg tttttccata ggctccgccc ccctgacgag catcacaaaa
     5701 atcgacgctc aagtcagagg tggcgaaacc cgacaggact ataaagatac caggcgtttc
     5761 cccctggaag ctccctcgtg cgctctcctg ttccgaccct gccgcttacc ggatacctgt
     5821 ccgcctttct cccttcggga agcgtggcgc tttctcaatg ctcacgctgt aggtatctca
     5881 gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc gttcagcccg
     5941 accgctgcgc cttatccggt aactatcgtc ttgagtccaa cccggtaaga cacgacttat
     6001 cgccactggc agcagccact ggtaacagga ttagcagagc gaggtatgta ggcggtgcta
     6061 cagagttctt gaagtggtgg cctaactacg gctacactag aaggacagta tttggtatct
     6121 gcgctctgct gaagccagtt accttcggaa aaagagttgg tagctcttga tccggcaaac
     6181 aaaccaccgc tggtagcggt ggtttttttg tttgcaagca gcagattacg cgcagaaaaa
     6241 aaggatctca agaagatcct ttgatctttt ctacggggtc tgacgctcag tggaacgaaa
     6301 actcacgtta agggattttg gtcatgagat tatcaaaaag gatcttcacc tagatccttt
     6361 taaattaaaa atgaagtttt aaatcaatct aaagtatata tgagtaaact tggtctgaca
     6421 gttaccaatg cttaatcagt gaggcaccta tctcagcgat ctgtctattt cgttcatcca
     6481 tagttgcctg actccccgtc gtgtagataa ctacgatacg ggagggctta ccatctggcc
     6541 ccagtgctgc aatgataccg cgagacccac gctcaccggc tccagattta tcagcaataa
     6601 accagccagc cggaagggcc gagcgcagaa gtggtcctgc aactttatcc gcctccatcc
     6661 agtctattaa ttgttgccgg gaagctagag taagtagttc gccagttaat agtttgcgca
     6721 acgttgttgc cattgctaca ggcatcgtgg tgtcacgctc gtcgtttggt atggcttcat
     6781 tcagctccgg ttcccaacga tcaaggcgag ttacatgatc ccccatgttg tgcaaaaaag
     6841 cggttagctc cttcggtcct ccgatcgttg tcagaagtaa gttggccgca gtgttatcac
     6901 tcatggttat ggcagcactg cataattctc ttactgtcat gccatccgta agatgctttt
     6961 ctgtgactgg tgagtactca accaagtcat tctgagaata gtgtatgcgg cgaccgagtt
     7021 gctcttgccc ggcgtcaata cgggataata ccgcgccaca tagcagaact ttaaaagtgc
     7081 tcatcattgg aaaacgttct tcggggcgaa aactctcaag gatcttaccg ctgttgagat
     7141 ccagttcgat gtaacccact cgtgcaccca actgatcttc agcatctttt actttcacca
     7201 gcgtttctgg gtgagcaaaa acaggaaggc aaaatgccgc aaaaaaggga ataagggcga
     7261 cacggaaatg ttgaatactc atactcttcc tttttcaata ttattgaagc atttatcagg
     7321 gttattgtct catgagcgga tacatatttg aatgtattta gaaaaataaa caaatagggg
     7381 ttccgcgcac atttccccga aaagtgccac ctg
//
