LOCUS       Exported                7407 bp DNA     circular SYN 10-AUG-2022
DEFINITION  .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7407)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Aug 10, 2022 from SnapGene Viewer 6.0.5
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..7407
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    34..356
                     /locus_tag="CMV"
                     /label=CMV
                     /note="color: #00ffff"
     misc_feature    170..189
                     /locus_tag="pCAGkanafwsequ"
                     /label=pCAGkanafwsequ
                     /note="color: #00ffff"
     misc_feature    386..661
                     /locus_tag="CAG enhancer"
                     /label=CAG enhancer
                     /note="color: #1460eb"
     misc_feature    662..1669
                     /locus_tag="rb beta globin stuff"
                     /label=rb beta globin stuff
                     /note="color: #808000"
     misc_feature    1678..1697
                     /locus_tag="CAGfw"
                     /label=CAGfw
                     /note="color: #00ffff"
     CDS             1720..4332
                     /codon_start=1
                     /locus_tag="Signal-flag-hmSEMA4D"
                     /label=Signal-flag-hmSEMA4D
                     /note="color: #ccffcc"
                     /translation="MRMCTPIRGLLMALAVMFGTAMADYKDDDDKFAPIPRITWEHREV
                     HLVQFHEPDIYNYSALLLSEDKDTLYIGAREAVFAVNALNISEKQHEVYWKVSEDKKAK
                     CAEKGKSKQTECLNYIRVLQPLSATSLYVCGTNAFQPACDHLNLTSFKFLGKNEDGKGR
                     CPFDPAHSYTSVMVDGELYSGTSYNFLGSEPIISRNSSHSPLRTEYAIPWLNEPSFVFA
                     DVIRKSPDSPDGEDDRVYFFFTEVSVEYEFVFRVLIPRIARVCKGDQGGLRTLQKKWTS
                     FLKARLICSRPDSGLVFNVLRDVFVLRSPGLKVPVFYALFTPQLNNVGLSAVCAYNLST
                     AEEVFSHGKYMQSTTVEQSHTKWVRYNGPVPKPRPGACIDSEARAANYTSSLNLPDKTL
                     QFVKDHPLMDDSVTPIDNRPRLIKKDVNYTQIVVDRTQALDGTVYDVMFVSTDRGALHK
                     AISLEHAVHIIEETQLFQDFEPVQTLLLSSKKGNRFVYAGSNSGVVPAPLAFCGKHGTC
                     EDCVLARDPYCAWSPPTATCVALHQTESPSRGLIQEMSGDASVCPDKSKGSYRQHFFKH
                     GGTAELKCSQKSNLARVFWKFQNGVLKAESPKYGLMGRKNLLIFNLSEGDSGVYQCLSE
                     ERVKNKTVFQVVAKHVLEVKVVPKPVVAPTLSVVQTEGSRIATKVLVASTQGSSPPTPA
                     VQATSSGAITLPPKPAPTGTSCEPKIVINTVPQLHSEKTMYLKSSDNRLLMSLFLFFFV
                     LFLCLFFYNCYKGYLPRQCLKFRSALLIGKKKPKSDFCDREQSLKETLVEPGSFSQQNG
                     EHPKPALDTGYETEQDTITSKVPTDREDSQRIDDLSARDKPFDVKCELKFADSDADGD"
     misc_feature    1720..1788
                     /label=signal peptide
                     /note="color: #a6acb3; direction: RIGHT"
     misc_feature    1789..1812
                     /locus_tag="flag"
                     /label=flag
                     /note="color: #800080"
     misc_feature    3232..3234
                     /label=497P
                     /note="color: #ff6600"
     misc_feature    4511..4533
                     /locus_tag="CAG rev"
                     /label=CAG rev
                     /note="color: #00ffff"
     primer_bind     complement(4906..4926)
                     /locus_tag="M13-rev"
                     /label=M13-rev
                     /note="color: #00ff00; direction: LEFT"
     misc_feature    4910..4926
                     /locus_tag="M13rev"
                     /label=M13rev
                     /note="color: #00ffff"
     misc_binding    complement(4932..4954)
                     /locus_tag="LacO"
                     /label=LacO
                     /note="color: #6495ed; direction: LEFT"
     misc_feature    5372..5392
                     /locus_tag="pCAGkanarevsequ"
                     /label=pCAGkanarevsequ
                     /note="color: #00ffff"
     rep_origin      complement(5639..6321)
                     /direction=LEFT
                     /locus_tag="ColE1 origin"
                     /label=ColE1 origin
                     /note="color: #808080"
     CDS             complement(6419..7078)
                     /locus_tag="AmpR"
                     /label=AmpR
                     /note="color: #ffff00; direction: LEFT"
ORIGIN
        1 gtcgacattg attattgact agttattaat agtaatcaat tacggggtca ttagttcata
       61 gcccatatat ggagttccgc gttacataac ttacggtaaa tggcccgcct ggctgaccgc
      121 ccaacgaccc ccgcccattg acgtcaataa tgacgtatgt tcccatagta acgccaatag
      181 ggactttcca ttgacgtcaa tgggtggact atttacggta aactgcccac ttggcagtac
      241 atcaagtgta tcatatgcca agtacgcccc ctattgacgt caatgacggt aaatggcccg
      301 cctggcatta tgcccagtac atgaccttat gggactttcc tacttggcag tacatctacg
      361 tattagtcat cgctattacc atgggtcgag gtgagcccca cgttctgctt cactctcccc
      421 atctcccccc cctccccacc cccaattttg tatttattta ttttttaatt attttgtgca
      481 gcgatggggg cggggggggg gggggcgcgc gccaggcggg gcggggcggg gcgaggggcg
      541 gggcggggcg aggcggagag gtgcggcggc agccaatcag agcggcgcgc tccgaaagtt
      601 tccttttatg gcgaggcggc ggcggcggcg gccctataaa aagcgaagcg cgcggcgggc
      661 gggagtcgct gcgttgcctt cgccccgtgc cccgctccgc gccgcctcgc gccgcccgcc
      721 ccggctctga ctgaccgcgt tactcccaca ggtgagcggg cgggacggcc cttctcctcc
      781 gggctgtaat tagcgcttgg tttaatgacg gctcgtttct tttctgtggc tgcgtgaaag
      841 ccttaaaggg ctccgggagg gccctttgtg cgggggggag cggctcgggg ggtgcgtgcg
      901 tgtgtgtgtg cgtggggagc gccgcgtgcg gcccgcgctg cccggcggct gtgagcgctg
      961 cgggcgcggc gcggggcttt gtgcgctccg cgtgtgcgcg aggggagcgc ggccgggggc
     1021 ggtgccccgc ggtgcggggg ggctgcgagg ggaacaaagg ctgcgtgcgg ggtgtgtgcg
     1081 tgggggggtg agcagggggt gtgggcgcgg cggtcgggct gtaacccccc cctgcacccc
     1141 cctccccgag ttgctgagca cggcccggct tcgggtgcgg ggctccgtgc ggggcgtggc
     1201 gcggggctcg ccgtgccggg cggggggtgg cggcaggtgg gggtgccggg cggggcgggg
     1261 ccgcctcggg ccggggaggg ctcgggggag gggcgcggcg gccccggagc gccggcggct
     1321 gtcgaggcgc ggcgagccgc agccattgcc ttttatggta atcgtgcgag agggcgcagg
     1381 gacttccttt gtcccaaatc tggcggagcc gaaatctggg aggcgccgcc gcaccccctc
     1441 tagcgggcgc gggcgaagcg gtgcggcgcc ggcaggaagg aaatgggcgg ggagggcctt
     1501 cgtgcgtcgc cgcgccgccg tccccttctc catctccagc ctcggggctg ccgcaggggg
     1561 acggctgcct tcggggggga cggggcaggg cggggttcgg cttctggcgt gtgaccggcg
     1621 gctctagagc ctctgctaac catgttcatg ccttcttctt tttcctacag ctcctgggca
     1681 acgtgctggt tattgtgctg tctcatcatt ttggcaaaga tgaggatgtg cacccccatt
     1741 agggggctgc tcatggccct tgcagtgatg tttgggacag cgatggcaga ttataaagat
     1801 gatgatgata aatttgcacc cataccccgg atcacctggg agcacagaga ggtgcacctg
     1861 gtgcagtttc atgagccaga catctacaac tactcagcct tgctgctgag cgaggacaag
     1921 gacaccttgt acataggtgc ccgggaggcg gtcttcgctg tgaacgcact caacatctcc
     1981 gagaagcagc atgaggtgta ttggaaggtc tcagaagaca aaaaagcaaa atgtgcagaa
     2041 aaggggaaat caaaacagac agagtgcctc aactacatcc gggtgctgca gccactcagc
     2101 gccacttccc tttacgtgtg tgggaccaac gcattccagc cggcctgtga ccacctgaac
     2161 ttaacatcct ttaagtttct ggggaaaaat gaagatggca aaggaagatg tccctttgac
     2221 ccagcacaca gctacacatc cgtcatggtt gatggagaac tttattcggg gacgtcgtat
     2281 aattttttgg gaagtgaacc catcatctcc cgaaattctt cccacagtcc tctgaggaca
     2341 gaatatgcaa tcccttggct gaacgagcct agtttcgtgt ttgctgacgt gatccgaaaa
     2401 agcccagaca gccccgacgg cgaggatgac agggtctact tcttcttcac ggaggtgtct
     2461 gtggagtatg agtttgtgtt cagggtgctg atcccacgga tagcaagagt gtgcaagggg
     2521 gaccagggcg gcctgaggac cttgcagaag aaatggacct ccttcctgaa agcccgactc
     2581 atctgctccc ggccagacag cggcttggtc ttcaatgtgc tgcgggatgt cttcgtgctc
     2641 aggtccccgg gcctgaaggt gcctgtgttc tatgcactct tcaccccaca gctgaacaac
     2701 gtggggctgt cggcagtgtg cgcctacaac ctgtccacag ccgaggaggt cttctcccac
     2761 gggaagtaca tgcagagcac cacagtggag cagtcccaca ccaagtgggt gcgctataat
     2821 ggcccggtac ccaagccgcg gcctggagcg tgcatcgaca gcgaggcacg ggccgccaac
     2881 tacaccagct ccttgaattt gccagacaag acgctgcagt tcgttaaaga ccaccctttg
     2941 atggatgact cggtaacccc aatagacaac aggcccaggt taatcaagaa agatgtgaac
     3001 tacacccaga tcgtggtgga ccggacccag gccctggatg ggactgtcta tgatgtcatg
     3061 tttgtcagca cagaccgggg agctctgcac aaagccatca gcctcgagca cgctgttcac
     3121 atcatcgagg agacccagct cttccaggac tttgagccag tccagaccct gctgctgtct
     3181 tcaaagaagg gcaacaggtt tgtctatgct ggctctaact cgggcgtggt ccccgccccg
     3241 ctggccttct gtgggaagca cggcacctgc gaggactgtg tgctggcgcg ggacccctac
     3301 tgcgcctgga gcccgcccac agcgacctgc gtggctctgc accagaccga gagccccagc
     3361 aggggtttga ttcaggagat gagcggcgat gcttctgtgt gcccggataa aagtaaagga
     3421 agttaccggc agcatttttt caagcacggt ggcacagcgg aactgaaatg ctcccaaaaa
     3481 tccaacctgg cccgggtctt ttggaagttc cagaatggcg tgttgaaggc cgagagcccc
     3541 aagtacggtc ttatgggcag aaaaaacttg ctcatcttca acttgtcaga aggagacagt
     3601 ggggtgtacc agtgcctgtc agaggagagg gttaagaaca aaacggtctt ccaagtggtc
     3661 gccaagcacg tcctggaagt gaaggtggtt ccaaagcccg tagtggcccc caccttgtca
     3721 gttgttcaga cagaaggtag taggattgcc accaaagtgt tggtggcatc cacccaaggg
     3781 tcttctcccc caaccccagc cgtgcaggcc acctcctccg gggccatcac ccttcctccc
     3841 aagcctgcgc ccaccggcac atcctgcgaa ccaaagatcg tcatcaacac ggtcccccag
     3901 ctccactcgg agaaaaccat gtatcttaag tccagcgaca accgcctcct catgtccctc
     3961 ttcctcttct tctttgttct cttcctctgc ctctttttct acaactgcta taagggatac
     4021 ctgcccagac agtgcttgaa attccgctcg gccctactaa ttgggaagaa gaagcccaag
     4081 tcagatttct gtgaccgtga gcagagcctg aaggagacgt tagtagagcc agggagcttc
     4141 tcccagcaga atggggagca ccccaagcca gccctggaca ccggctatga gaccgagcaa
     4201 gacaccatca ccagcaaagt ccccacggat agggaggact cacagaggat cgacgacctt
     4261 tctgccaggg acaagccctt tgacgtcaag tgtgagctga agttcgctga ctcagacgca
     4321 gatggagact gacctcgagg atatcgcggc cgcactcctc aggtgcaggc tgcctatcag
     4381 aaggtggtgg ctggtgtggc caatgccctg gctcacaaat accactgaga tctttttccc
     4441 tctgccaaaa attatgggga catcatgaag ccccttgagc atctgacttc tggctaataa
     4501 aggaaattta ttttcattgc aatagtgtgt tggaattttt tgtgtctctc actcggaagg
     4561 acatatggga gggcaaatca tttaaaacat cagaatgagt atttggttta gagtttggca
     4621 acatatgcca tatgctggct gccatgaaca aaggtggcta taaagaggtc atcagtatat
     4681 gaaacagccc cctgctgtcc attccttatt ccatagaaaa gccttgactt gaggttagat
     4741 tttttttata ttttgttttg tgttattttt ttctttaaca tccctaaaat tttccttaca
     4801 tgttttacta gccagatttt tcctcctctc ctgactactc ccagtcatag ctgtccctct
     4861 tctcttatga agatccctcg acctgcagcc caagcttggc gtaatcatgg tcatagctgt
     4921 ttcctgtgtg aaattgttat ccgctcacaa ttccacacaa catacgagcc ggaagcataa
     4981 agtgtaaagc ctggggtgcc taatgagtga gctaactcac attaattgcg ttgcgctcac
     5041 tgcccgcttt ccagtcggga aacctgtcgt gccagcggat ccgcatctca attagtcagc
     5101 aaccatagtc ccgcccctaa ctccgcccat cccgccccta actccgccca gttccgccca
     5161 ttctccgccc catggctgac taattttttt tatttatgca gaggccgagg ccgcctcggc
     5221 ctctgagcta ttccagaagt agtgaggagg cttttttgga ggcctaggct tttgcaaaaa
     5281 gctaacttgt ttattgcagc ttataatggt tacaaataaa gcaatagcat cacaaatttc
     5341 acaaataaag catttttttc actgcattct agttgtggtt tgtccaaact catcaatgta
     5401 tcttatcatg tctggatccg ctgcattaat gaatcggcca acgcgcgggg agaggcggtt
     5461 tgcgtattgg gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc
     5521 tgcggcgagc ggtatcagct cactcaaagg cggtaatacg gttatccaca gaatcagggg
     5581 ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg
     5641 ccgcgttgct ggcgtttttc cataggctcc gcccccctga cgagcatcac aaaaatcgac
     5701 gctcaagtca gaggtggcga aacccgacag gactataaag ataccaggcg tttccccctg
     5761 gaagctccct cgtgcgctct cctgttccga ccctgccgct taccggatac ctgtccgcct
     5821 ttctcccttc gggaagcgtg gcgctttctc aatgctcacg ctgtaggtat ctcagttcgg
     5881 tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct
     5941 gcgccttatc cggtaactat cgtcttgagt ccaacccggt aagacacgac ttatcgccac
     6001 tggcagcagc cactggtaac aggattagca gagcgaggta tgtaggcggt gctacagagt
     6061 tcttgaagtg gtggcctaac tacggctaca ctagaaggac agtatttggt atctgcgctc
     6121 tgctgaagcc agttaccttc ggaaaaagag ttggtagctc ttgatccggc aaacaaacca
     6181 ccgctggtag cggtggtttt tttgtttgca agcagcagat tacgcgcaga aaaaaaggat
     6241 ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc tcagtggaac gaaaactcac
     6301 gttaagggat tttggtcatg agattatcaa aaaggatctt cacctagatc cttttaaatt
     6361 aaaaatgaag ttttaaatca atctaaagta tatatgagta aacttggtct gacagttacc
     6421 aatgcttaat cagtgaggca cctatctcag cgatctgtct atttcgttca tccatagttg
     6481 cctgactccc cgtcgtgtag ataactacga tacgggaggg cttaccatct ggccccagtg
     6541 ctgcaatgat accgcgagac ccacgctcac cggctccaga tttatcagca ataaaccagc
     6601 cagccggaag ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc atccagtcta
     6661 ttaattgttg ccgggaagct agagtaagta gttcgccagt taatagtttg cgcaacgttg
     6721 ttgccattgc tacaggcatc gtggtgtcac gctcgtcgtt tggtatggct tcattcagct
     6781 ccggttccca acgatcaagg cgagttacat gatcccccat gttgtgcaaa aaagcggtta
     6841 gctccttcgg tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta tcactcatgg
     6901 ttatggcagc actgcataat tctcttactg tcatgccatc cgtaagatgc ttttctgtga
     6961 ctggtgagta ctcaaccaag tcattctgag aatagtgtat gcggcgaccg agttgctctt
     7021 gcccggcgtc aatacgggat aataccgcgc cacatagcag aactttaaaa gtgctcatca
     7081 ttggaaaacg ttcttcgggg cgaaaactct caaggatctt accgctgttg agatccagtt
     7141 cgatgtaacc cactcgtgca cccaactgat cttcagcatc ttttactttc accagcgttt
     7201 ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg gcgacacgga
     7261 aatgttgaat actcatactc ttcctttttc aatattattg aagcatttat cagggttatt
     7321 gtctcatgag cggatacata tttgaatgta tttagaaaaa taaacaaata ggggttccgc
     7381 gcacatttcc ccgaaaagtg ccacctg
//
