LOCUS       Exported               10550 bp ds-DNA     circular SYN 12-AUG-2022
DEFINITION  Target plasmid containing PSP1.
ACCESSION   .
VERSION     .
KEYWORDS    pEcCas-2.0
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10550)
  TITLE     RNA-guided DNA insertion with CRISPR-associated transposases
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 10550)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Friday, Aug 12, 2022 from SnapGene 5.0.8
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..10550
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     rep_origin      complement(422..967)
                     /direction=LEFT
                     /label=p15A ori
                     /note="Plasmids containing the medium-copy-number p15A 
                     origin of replication can be propagated in E. coli cells 
                     that contain a second plasmid with the ColE1 origin.
                     "
                     /note="color: #ffff00"
     primer_bind     450..470
                     /label=P15A-F
                     /note="color: black; sequence: catcacgaaatctgacgctca"
     primer_bind     complement(699..720)
                     /label=P15A-R
                     /note="color: black; sequence: ggactcaagacgatagttaccg"
     primer_bind     781..802
                     /label=pTargetseq-F
                     /note="color: black; sequence: gttagtcttgaagtcatgcgcc"
     primer_bind     1141..1161
                     /label=Cas+red-pA-up
                     /note="color: black; sequence: acatacgcagcgatttccaac"
     misc_feature    1173..1192
                     /label=up
                     /note="color: #a6acb3"
     primer_bind     complement(1173..1192)
                     /label=p15ABack-R
                     /note="color: black; sequence: aggacccgcattaaagcttc"
     primer_bind     complement(1186..1209)
                     /label=Cas+red-pC-dn
                     /note="color: black; sequence: Gtaatacaaggggtgttaggaccc"
     misc_feature    1314..1328
                     /label='tracrR171
                     /note="/vntifkey=21"
                     /note="color: #a6acb3"
     CDS             1540..5646
                     /codon_start=1
                     /locus_tag="M5005_Spy_0769"
                     /product="hypothetical protein"
                     /label=csn1
                     /note="hypothetical protein"
                     /note="/vntifkey=4"
                     /note="color: #993366"
                     /db_xref="GI:71910582"
                     /db_xref="GeneID:3572134"
                     /protein_id="YP_282132.1"
                     /translation="MDKKYSIGLDIGTNSVGWAVITDDYKVPSKKFKVLGNTDRHSIKK
                     NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES
                     FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK
                     FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL
                     ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ
                     IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR
                     QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL
                     RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG
                     NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY
                     FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFD
                     SVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEER
                     LKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFM
                     QLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGR
                     HKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLY
                     LYYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVP
                     SEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKH
                     VAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYL
                     NAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKT
                     EITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSK
                     ESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGI
                     TIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNE
                     LALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD
                     ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVL
                     DATLIHQSITGLYETRIDLSQLGGD"
     primer_bind     complement(5756..5798)
                     /label=Cas+red-pA-dn
                     /note="color: black; sequence: 
                     atgtagccgtcaagttgtcataataaatcgatgcaggtggcaC"
     primer_bind     5756..5798
                     /label=Red-up
                     /note="color: black; sequence: 
                     Gtgccacctgcatcgatttattatgacaacttgacggctacat"
     CDS             complement(5776..6654)
                     /codon_start=1
                     /label=araC
                     /note="araC protein"
                     /note="/vntifkey=4"
                     /note="color: #993366"
                     /translation="MAEAQNDPLLPGYSFNAHLVAGLTPIEANGYLDFFIDRPLGMKGY
                     ILNLTIRGQGVVKNQGREFVCRPGDILLFPPGEIHHYGRHPEAREWYHQWVYFRPRAYW
                     HEWLNWPSIFANTGFFRPDEAHQPHFSDLFGQIINAGQGEGRYSELLAINLLEQLLLRR
                     MEAINESLHPPMDNRVREACQYISDHLADSNFDIASVAQHVCLSPSRLSHLFRQQLGIS
                     VLSWREDQRISQAKLLLSTTRMPIATVGRNVGFDDQLYFSRVFKKCTGASPSEFRAGCE
                     EKVNDVAVKLS"
     promoter        6681..6965
                     /gene="araBAD"
                     /label=araBAD promoter
                     /note="promoter of the L-arabinose operon of E. coli; the 
                     araC regulatory gene is transcribed in the opposite 
                     direction (Guzman et al., 1995)"
                     /note="color: #ffffff; direction: RIGHT"
     RBS             6992..7000
                     /label=Shine-Dalgarno sequence
                     /note="full consensus sequence for ribosome-binding sites 
                     upstream of start codons in E. coli; complementary to a 
                     region in the 3' end of the 16S rRNA (Chen et al., 1994)"
                     /note="color: #a6acb3"
     CDS             7009..7425
                     /codon_start=1
                     /label=gam
                     /note="gam protein"
                     /note="/vntifkey=4"
                     /note="color: #993366"
                     /translation="MDINTETEIKQKHSLTPFPVFLISPAFRGRYFHSYFRSSAMNAYY
                     IQDRLEAQSWARHYQQLAREEKEAELADDMEKGLPQHLFESLCIDHLQRHGASKKSITR
                     AFDDDVEFQERMAEHIRYMVETIAHHQVDIDSEV"
     CDS             7431..8216
                     /codon_start=1
                     /label=bet
                     /note="bet protein"
                     /note="/vntifkey=4"
                     /note="color: #993366"
                     /translation="MSTALATLAGKLAERVGMDSVDPQELITTLRQTAFKGDASDAQFI
                     ALLIVANQYGLNPWTKEIYAFPDKQNGIVPVVGVDGWSRIINENQQFDGMDFEQDNESC
                     TCRIYRKDRNHPICVTEWMDECRREPFKTREGREITGPWQSHPKRMLRHKAMIQCARLA
                     FGFAGIYDKDEAERIVENTAYTAERQPERDITPVNDETMQEINTLLIALDKTWDDDLLP
                     LCSQIFRRDIRASSELTQAEAVKALGFLKQKAAEQKVAA"
     CDS             8213..8893
                     /codon_start=1
                     /label=exonuclease
                     /note="exonuclease"
                     /note="/vntifkey=4"
                     /note="color: #993366"
                     /translation="MTPDIILQRTGIDVRAVEQGDDAWHKLRLGVITASEVHNVIAKPR
                     SGKKWPDMKMSYFHTLLAEVCTGVAPEVNAKALAWGKQYENDARTLFEFTSGVNVTESP
                     IIYRDESMRTACSPDGLCSDGNGLELKCPFTSRDFMKFRLGGFEAIKSAYMAQVQYSMW
                     VTRKNAWYFANYDPRMKREGLHYVVIERDEKYMASFDEIVPEFIEKMDEALAEIGFVFG
                     EQWR"
     CDS             8890..9072
                     /codon_start=1
                     /label=orf60a
                     /note="orf60a protein"
                     /note="/vntifkey=4"
                     /note="color: #993366"
                     /translation="MTHPHDNIRVGAITFVYSVTKRGWVFPGLSVIRNPLKAQRLAEEI
                     NNKRGAVCTKHLLLS"
     terminator      8894..9138
                     /label=lambda tL3 terminator
                     /note="transcription terminator tL3 from phage lambda"
                     /note="color: #ffffff"
     primer_bind     9147..9187
                     /label=Cas+red-pC-up
                     /note="color: black; sequence: 
                     Gaagaatccatgggtatggacagtggatcctctacgccgga"
     primer_bind     complement(9147..9187)
                     /label=Red-dn
                     /note="color: black; sequence: 
                     Tccggcgtagaggatccactgtccatacccatggattcttc"
     misc_feature    9169..9195
                     /label=dn
                     /note="color: #a6acb3"
     primer_bind     9169..9187
                     /label=P15ABack-F
                     /note="color: black; sequence: gtggatcctctacgccgga"
     primer_bind     complement(9234..9253)
                     /label=pBRrevBam
                     /note="color: black; sequence: ggtgatgtcggcgatatagg"
     CDS             complement(9684..10343)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /note="color: #ccffcc"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     promoter        complement(10344..10446)
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding 
                     chloramphenicol acetyltransferase"
                     /note="color: #ffffff; direction: LEFT"
ORIGIN
        1 ggcgcaaagt gcgtcgggtg atgctgccaa cttactgatt tagtgtatga tggtgttttt
       61 gaggtgctcc agtggcttct gtttctatca gctgtccctc ctgttcagct actgacgggg
      121 tggtgcgtaa cggcaaaagc accgccggac atcagcgcta gcggagtgta tactggctta
      181 ctatgttggc actgatgagg gtgtcagtga agtgcttcat gtggcaggag aaaaaaggct
      241 gcaccggtgc gtcagcagaa tatgtgatac aggatatatt ccgcttcctc gctcactgac
      301 tcgctacgct cggtcgttcg actgcggcga gcggaaatgg cttacgaacg gggcggagat
      361 ttcctggaag atgccaggaa gatacttaac agggaagtga gagggccgcg gcaaagccgt
      421 ttttccatag gctccgcccc cctgacaagc atcacgaaat ctgacgctca aatcagtggt
      481 ggcgaaaccc gacaggacta taaagatacc aggcgtttcc ccctggcggc tccctcgtgc
      541 gctctcctgt tcctgccttt cggtttaccg gtgtcattcc gctgttatgg ccgcgtttgt
      601 ctcattccac gcctgacact cagttccggg taggcagttc gctccaagct ggactgtatg
      661 cacgaacccc ccgttcagtc cgaccgctgc gccttatccg gtaactatcg tcttgagtcc
      721 aacccggaaa gacatgcaaa agcaccactg gcagcagcca ctggtaattg atttagagga
      781 gttagtcttg aagtcatgcg ccggttaagg ctaaactgaa aggacaagtt ttggtgactg
      841 cgctcctcca agccagttac ctcggttcaa agagttggta gctcagagaa ccttcgaaaa
      901 accgccctgc aaggcggttt tttcgttttc agagcaagag attacgcgca gaccaaaacg
      961 atctcaagaa gatcatctta ttaatatacg tagtatctac gatacgtagt atctacgata
     1021 cgtaggaggt tgagaagtca tttaataagg ccactgttaa acgtagatat atctacgcgt
     1081 agatatatct acgatacgta gcggccgacg cgctgggcta cgtcttaatg ggcagcgccc
     1141 acatacgcag cgatttccaa cagcttgagg cggaagcttt aatgcgggtc ctaacacccc
     1201 ttgtattact gtttatgtaa gcagacagtt ttattgttca tgatgatata tttttatctt
     1261 gtgcaatgta acatcagaga ttttgagaca caacgtggct ttccctgcag ctgaacaata
     1321 cttaatacta tagaatgata acaaaataaa ctacttttta aaagaatttt gtgttataat
     1381 ctatttatta ttaagtattg ggtaatattt tttgaagaga tattttgaaa aagaaaaatt
     1441 aaagcatatt aaactaattt cggaggtcat taaaactatt attgaaatca tcaaactcat
     1501 tatggattta atttaaactt tttattttag gaggcaaaaa tggataagaa atactcaata
     1561 ggcttagata tcggcacaaa tagcgtcgga tgggcggtga tcactgatga ttataaggtt
     1621 ccgtctaaaa agttcaaggt tctgggaaat acagaccgcc acagtatcaa aaaaaatctt
     1681 ataggggctc ttttatttga cagtggagag acagcggaag cgactcgtct caaacggaca
     1741 gctcgtagaa ggtatacacg tcggaagaat cgtatttgtt atctacagga gattttttca
     1801 aatgagatgg cgaaagtaga tgatagtttc tttcatcgac ttgaagagtc ttttttggtg
     1861 gaagaagaca agaagcatga acgtcatcct atttttggaa atatagtaga tgaagttgct
     1921 tatcatgaga aatatccaac tatctatcat ctgcgaaaaa aattggtaga ttctactgat
     1981 aaagcggatt tgcgcttaat ctatttggcc ttagcgcata tgattaagtt tcgtggtcat
     2041 tttttgattg agggagattt aaatcctgat aatagtgatg tggacaaact atttatccag
     2101 ttggtacaaa cctacaatca attatttgaa gaaaacccta ttaacgcaag tggagtagat
     2161 gctaaagcga ttctttctgc acgattgagt aaatcaagac gattagaaaa tctcattgct
     2221 cagctccccg gtgagaagaa aaatggctta tttgggaatc tcattgcttt gtcattgggt
     2281 ttgaccccta attttaaatc aaattttgat ttggcagaag atgctaaatt acagctttca
     2341 aaagatactt acgatgatga tttagataat ttattggcgc aaattggaga tcaatatgct
     2401 gatttgtttt tggcagctaa gaatttatca gatgctattt tactttcaga tatcctaaga
     2461 gtaaatactg aaataactaa ggctccccta tcagcttcaa tgattaaacg ctacgatgaa
     2521 catcatcaag acttgactct tttaaaagct ttagttcgac aacaacttcc agaaaagtat
     2581 aaagaaatct tttttgatca atcaaaaaac ggatatgcag gttatattga tgggggagct
     2641 agccaagaag aattttataa atttatcaaa ccaattttag aaaaaatgga tggtactgag
     2701 gaattattgg tgaaactaaa tcgtgaagat ttgctgcgca agcaacggac ctttgacaac
     2761 ggctctattc cccatcaaat tcacttgggt gagctgcatg ctattttgag aagacaagaa
     2821 gacttttatc catttttaaa agacaatcgt gagaagattg aaaaaatctt gacttttcga
     2881 attccttatt atgttggtcc attggcgcgt ggcaatagtc gttttgcatg gatgactcgg
     2941 aagtctgaag aaacaattac cccatggaat tttgaagaag ttgtcgataa aggtgcttca
     3001 gctcaatcat ttattgaacg catgacaaac tttgataaaa atcttccaaa tgaaaaagta
     3061 ctaccaaaac atagtttgct ttatgagtat tttacggttt ataacgaatt gacaaaggtc
     3121 aaatatgtta ctgaaggaat gcgaaaacca gcatttcttt caggtgaaca gaagaaagcc
     3181 attgttgatt tactcttcaa aacaaatcga aaagtaaccg ttaagcaatt aaaagaagat
     3241 tatttcaaaa aaatagaatg ttttgatagt gttgaaattt caggagttga agatagattt
     3301 aatgcttcat taggtaccta ccatgatttg ctaaaaatta ttaaagataa agattttttg
     3361 gataatgaag aaaatgaaga tatcttagag gatattgttt taacattgac cttatttgaa
     3421 gatagggaga tgattgagga aagacttaaa acatatgctc acctctttga tgataaggtg
     3481 atgaaacagc ttaaacgtcg ccgttatact ggttggggac gtttgtctcg aaaattgatt
     3541 aatggtatta gggataagca atctggcaaa acaatattag attttttgaa atcagatggt
     3601 tttgccaatc gcaattttat gcagctgatc catgatgata gtttgacatt taaagaagac
     3661 attcaaaaag cacaagtgtc tggacaaggc gatagtttac atgaacatat tgcaaattta
     3721 gctggtagcc ctgctattaa aaaaggtatt ttacagactg taaaagttgt tgatgaattg
     3781 gtcaaagtaa tggggcggca taagccagaa aatatcgtta ttgaaatggc acgtgaaaat
     3841 cagacaactc aaaagggcca gaaaaattcg cgagagcgta tgaaacgaat cgaagaaggt
     3901 atcaaagaat taggaagtca gattcttaaa gagcatcctg ttgaaaatac tcaattgcaa
     3961 aatgaaaagc tctatctcta ttatctccaa aatggaagag acatgtatgt ggaccaagaa
     4021 ttagatatta atcgtttaag tgattatgat gtcgatcaca ttgttccaca aagtttcctt
     4081 aaagacgatt caatagacaa taaggtctta acgcgttctg ataaaaatcg tggtaaatcg
     4141 gataacgttc caagtgaaga agtagtcaaa aagatgaaaa actattggag acaacttcta
     4201 aacgccaagt taatcactca acgtaagttt gataatttaa cgaaagctga acgtggaggt
     4261 ttgagtgaac ttgataaagc tggttttatc aaacgccaat tggttgaaac tcgccaaatc
     4321 actaagcatg tggcacaaat tttggatagt cgcatgaata ctaaatacga tgaaaatgat
     4381 aaacttattc gagaggttaa agtgattacc ttaaaatcta aattagtttc tgacttccga
     4441 aaagatttcc aattctataa agtacgtgag attaacaatt accatcatgc ccatgatgcg
     4501 tatctaaatg ccgtcgttgg aactgctttg attaagaaat atccaaaact tgaatcggag
     4561 tttgtctatg gtgattataa agtttatgat gttcgtaaaa tgattgctaa gtctgagcaa
     4621 gaaataggca aagcaaccgc aaaatatttc ttttactcta atatcatgaa cttcttcaaa
     4681 acagaaatta cacttgcaaa tggagagatt cgcaaacgcc ctctaatcga aactaatggg
     4741 gaaactggag aaattgtctg ggataaaggg cgagattttg ccacagtgcg caaagtattg
     4801 tccatgcccc aagtcaatat tgtcaagaaa acagaagtac agacaggcgg attctccaag
     4861 gagtcaattt taccaaaaag aaattcggac aagcttattg ctcgtaaaaa agactgggat
     4921 ccaaaaaaat atggtggttt tgatagtcca acggtagctt attcagtcct agtggttgct
     4981 aaggtggaaa aagggaaatc gaagaagtta aaatccgtta aagagttact agggatcaca
     5041 attatggaaa gaagttcctt tgaaaaaaat ccgattgact ttttagaagc taaaggatat
     5101 aaggaagtta aaaaagactt aatcattaaa ctacctaaat atagtctttt tgagttagaa
     5161 aacggtcgta aacggatgct ggctagtgcc ggagaattac aaaaaggaaa tgagctggct
     5221 ctgccaagca aatatgtgaa ttttttatat ttagctagtc attatgaaaa gttgaagggt
     5281 agtccagaag ataacgaaca aaaacaattg tttgtggagc agcataagca ttatttagat
     5341 gagattattg agcaaatcag tgaattttct aagcgtgtta ttttagcaga tgccaattta
     5401 gataaagttc ttagtgcata taacaaacat agagacaaac caatacgtga acaagcagaa
     5461 aatattattc atttatttac gttgacgaat cttggagctc ccgctgcttt taaatatttt
     5521 gatacaacaa ttgatcgtaa acgatatacg tctacaaaag aagttttaga tgccactctt
     5581 atccatcaat ccatcactgg tctttatgaa acacgcattg atttgagtca gctaggaggt
     5641 gactgaagta tattttagat gaagattatt tcttaatcta gacatgagcg gatacatatt
     5701 tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc
     5761 acctgcatcg atttattatg acaacttgac ggctacatca ttcacttttt cttcacaacc
     5821 ggcacggaac tcgctcgggc tggccccggt gcatttttta aatacccgcg agaaatagag
     5881 ttgatcgtca aaaccaacat tgcgaccgac ggtggcgata ggcatccggg tggtgctcaa
     5941 aagcagcttc gcctggctga tacgttggtc ctcgcgccag cttaagacgc taatccctaa
     6001 ctgctggcgg aaaagatgtg acagacgcga cggcgacaag caaacatgct gtgcgacgct
     6061 ggcgatatca aaattgctgt ctgccaggtg atcgctgatg tactgacaag cctcgcgtac
     6121 ccgattatcc atcggtggat ggagcgactc gttaatcgct tccatgcgcc gcagtaacaa
     6181 ttgctcaagc agatttatcg ccagcagctc cgaatagcgc ccttcccctt gcccggcgtt
     6241 aatgatttgc ccaaacaggt cgctgaaatg cggctggtgc gcttcatccg ggcgaaagaa
     6301 ccccgtattg gcaaatattg acggccagtt aagccattca tgccagtagg cgcgcggacg
     6361 aaagtaaacc cactggtgat accattcgcg agcctccgga tgacgaccgt agtgatgaat
     6421 ctctcctggc gggaacagca aaatatcacc cggtcggcaa acaaattctc gtccctgatt
     6481 tttcaccacc ccctgaccgc gaatggtgag attgagaata taacctttca ttcccagcgg
     6541 tcggtcgata aaaaaatcga gataaccgtt ggcctcaatc ggcgttaaac ccgccaccag
     6601 atgggcatta aacgagtatc ccggcagcag gggatcattt tgcgcttcag ccatactttt
     6661 catactcccg ccattcagag aagaaaccaa ttgtccatat tgcatcagac attgccgtca
     6721 ctgcgtcttt tactggctct tctcgctaac caaaccggta accccgctta ttaaaagcat
     6781 tctgtaacaa agcgggacca aagccatgac aaaaacgcgt aacaaaagtg tctataatca
     6841 cggcagaaaa gtccacattg attatttgca cggcgtcaca ctttgctatg ccatagcatt
     6901 tttatccata agattagcgg atcctacctg acgcttttta tcgcaactct ctactgtttc
     6961 tccatacccg tttttttggg aattcgagct ctaaggaggt tataaaaaat ggatattaat
     7021 actgaaactg agatcaagca aaagcattca ctaaccccct ttcctgtttt cctaatcagc
     7081 ccggcatttc gcgggcgata ttttcacagc tatttcagga gttcagccat gaacgcttat
     7141 tacattcagg atcgtcttga ggctcagagc tgggcgcgtc actaccagca gctcgcccgt
     7201 gaagagaaag aggcagaact ggcagacgac atggaaaaag gcctgcccca gcacctgttt
     7261 gaatcgctat gcatcgatca tttgcaacgc cacggggcca gcaaaaaatc cattacccgt
     7321 gcgtttgatg acgatgttga gtttcaggag cgcatggcag aacacatccg gtacatggtt
     7381 gaaaccattg ctcaccacca ggttgatatt gattcagagg tataaaacga atgagtactg
     7441 cactcgcaac gctggctggg aagctggctg aacgtgtcgg catggattct gtcgacccac
     7501 aggaactgat caccactctt cgccagacgg catttaaagg tgatgccagc gatgcgcagt
     7561 tcatcgcatt actgatcgtt gccaaccagt acggccttaa tccgtggacg aaagaaattt
     7621 acgcctttcc tgataagcag aatggcatcg ttccggtggt gggcgttgat ggctggtccc
     7681 gcatcatcaa tgaaaaccag cagtttgatg gcatggactt tgagcaggac aatgaatcct
     7741 gtacatgccg gatttaccgc aaggaccgta atcatccgat ctgcgttacc gaatggatgg
     7801 atgaatgccg ccgcgaacca ttcaaaactc gcgaaggcag agaaatcacg gggccgtggc
     7861 agtcgcatcc caaacggatg ttacgtcata aagccatgat tcagtgtgcc cgtctggcct
     7921 tcggatttgc tggtatctat gacaaggatg aagccgagcg cattgtcgaa aatactgcat
     7981 acactgcaga acgtcagccg gaacgcgaca tcactccggt taacgatgaa accatgcagg
     8041 agattaacac tctgctgatc gccctggata aaacatggga tgacgactta ttgccgctct
     8101 gttcccagat atttcgccgc gacattcgtg catcgtcaga actgacacag gccgaagcag
     8161 taaaagctct tggattcctg aaacagaaag ccgcagagca gaaggtggca gcatgacacc
     8221 ggacattatc ctgcagcgta ccgggatcga tgtgagagct gtcgaacagg gggatgatgc
     8281 gtggcacaaa ttacggctcg gcgtcatcac cgcttcagaa gttcacaacg tgatagcaaa
     8341 accccgctcc ggaaagaagt ggcctgacat gaaaatgtcc tacttccaca ccctgcttgc
     8401 tgaggtttgc accggtgtgg ctccggaagt taacgctaaa gcactggcct ggggaaaaca
     8461 gtacgagaac gacgccagaa ccctgtttga attcacttcc ggcgtgaatg ttactgaatc
     8521 cccgatcatc tatcgcgacg aaagtatgcg taccgcctgc tctcccgatg gtttatgcag
     8581 tgacggcaac ggccttgaac tgaaatgccc gtttacctcc cgggatttca tgaagttccg
     8641 gctcggtggt ttcgaggcca taaagtcagc ttacatggcc caggtgcagt acagcatgtg
     8701 ggtgacgcga aaaaatgcct ggtactttgc caactatgac ccgcgtatga agcgtgaagg
     8761 cctgcattat gtcgtgattg agcgggatga aaagtacatg gcgagttttg acgagatcgt
     8821 gccggagttc atcgaaaaaa tggacgaggc actggctgaa attggttttg tatttgggga
     8881 gcaatggcga tgacgcatcc tcacgataat atccgggtag gcgcaatcac tttcgtctac
     8941 tccgttacaa agcgaggctg ggtatttccc ggcctttctg ttatccgaaa tccactgaaa
     9001 gcacagcggc tggctgagga gataaataat aaacgagggg ctgtatgcac aaagcatctt
     9061 ctgttgagtt aagaacgagt atcgagatgg cacatagcct tgctcaaatt ggaatcaggt
     9121 ttgtgccaat accagtagaa acagacgaag aatccatggg tatggacagt ggatcctcta
     9181 cgccggacgc atcgtggccg gcatcaccgg cgccacaggt gcggttgctg gcgcctatat
     9241 cgccgacatc accgatgggg aagatcgggc ctgccaccat acccacgccg aaacaagcgc
     9301 cctgcaccat tatgttccgg atctgcatcg caggatgctg ctggctaccc tgtggaacac
     9361 ctacatctgt attaacgaag cgctaaccgt ttttatcagg ctctgggagg cagaataaat
     9421 gatcatatcg tcaattatta cctccacggg gagagcctga gcaaactggc ctcaggcatt
     9481 tgagaagcac acggtcacac tgcttccggt agtcaataaa ccggtaaacc agcaatagac
     9541 ataagcggct atttaacgac cctgccctga accgacgacc gggtcgaatt tgctttcgaa
     9601 tttctgccat tcatccgctt attatcactt attcaggcgt agcaaccagg cgtttaaggg
     9661 caccaataac tgccttaaaa aaattacgcc ccgccctgcc actcatcgca gtactgttgt
     9721 aattcattaa gcattctgcc gacatggaag ccatcacaaa cggcatgatg aacctgaatc
     9781 gccagcggca tcagcacctt gtcgccttgc gtataatatt tgcccatggt gaaaacgggg
     9841 gcgaagaagt tgtccatatt ggccacgttt aaatcaaaac tggtgaaact cacccaggga
     9901 ttggctgaga cgaaaaacat attctcaata aaccctttag ggaaataggc caggttttca
     9961 ccgtaacacg ccacatcttg cgaatatatg tgtagaaact gccggaaatc gtcgtggtat
    10021 tcactccaga gcgatgaaaa cgtttcagtt tgctcatgga aaacggtgta acaagggtga
    10081 acactatccc atatcaccag ctcaccgtct ttcattgcca tacggaattc cggatgagca
    10141 ttcatcaggc gggcaagaat gtgaataaag gccggataaa acttgtgctt atttttcttt
    10201 acggtcttta aaaaggccgt aatatccagc tgaacggtct ggttataggt acattgagca
    10261 actgactgaa atgcctcaaa atgttcttta cgatgccatt gggatatatc aacggtggta
    10321 tatccagtga tttttttctc cattttagct tccttagctc ctgaaaatct cgataactca
    10381 aaaaatacgc ccggtagtga tcttatttca ttatggtgaa agttggaacc tcttacgtgc
    10441 cgatcaacgt ctcattttcg ccaaaagttg gcccagggct tcccggtatc aacagggaca
    10501 ccaggattta tttattctgc gaagtgatct tccgtcacag gtatttattc
//
