LOCUS       pVRc-pBAD-N-Venus-V150A 5453 bp ds-DNA     circular     14-APR-2021
DEFINITION  .
KEYWORDS    "marker:CmR"                                                       
FEATURES             Location/Qualifiers
     terminator      26..112
                     /label="rrnB T1 terminator"
                     /ApEinfo_revcolor=#c6c9d1
                     /ApEinfo_fwdcolor=#c6c9d1
     terminator      204..231
                     /label="rrnB T2 terminator"
                     /ApEinfo_revcolor=#c6c9d1
                     /ApEinfo_fwdcolor=#c6c9d1
     promoter        250..341
                     /label="AmpR promoter"
                     /ApEinfo_revcolor=#c6c9d1
                     /ApEinfo_fwdcolor=#c6c9d1
     CDS             511..1170
                     /label="CmR"
                     /ApEinfo_revcolor=#b7e6d7
                     /ApEinfo_fwdcolor=#b7e6d7
     rep_origin      1312..1767
                     /label="f1 ori"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     rep_origin      complement(2587..3132)
                     /label="p15A ori"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     CDS             complement(3403..4281)
                     /label="araC"
                     /ApEinfo_revcolor=#993366
                     /ApEinfo_fwdcolor=#993366
     promoter        4308..4592
                     /label="araBAD promoter"
                     /ApEinfo_revcolor=#c6c9d1
                     /ApEinfo_fwdcolor=#c6c9d1
     CDS             4627..4674
                     /label="Translation 4627-4674"
     misc_feature    4675..5139
                     /label="N-Venus"
                     /ApEinfo_revcolor=#b4abac
                     /ApEinfo_fwdcolor=#b4abac
     CDS             4675..5159
                     /label="Translation 4675-5159"
     misc_feature    5125..5127
                     /label="V150A"
                     /ApEinfo_revcolor=#d6b295
                     /ApEinfo_fwdcolor=#d6b295
     misc_feature    5140..5159
                     /label="12AA Linker"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     CDS             5158..5159
                     /label="Translation 5158-5159"
     CDS             5158..5271
                     /label="Translation 5158-5271"
     misc_feature    5167..5172
                     /label="gsg LINKER"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     misc_feature    5173..5262
                     /label="apCC-Di"
                     /ApEinfo_revcolor=#f8d3a9
                     /ApEinfo_fwdcolor=#f8d3a9
ORIGIN
        1 GCGAGAGTAG GGAACTGCCA GGCATCAAAT AAAACGAAAG GCTCAGTCGA AAGACTGGGC
       61 CTTTCGTTTT ATCTGTTGTT TGTCGGTGAA CGCTCTCCTG AGTAGGACAA ATCCGCCGGG
      121 AGCGGATTTG AACGTTGCGA AGCAACGGCC CGGAGGGTGG CGGGCAGGAC GCCCGCCATA
      181 AACTGCCAGG CATCAAATTA AGCAGAAGGC CATCCTGACG GATGGCCTTT TTGCGTTTCT
      241 ACAAACTCTT TTGTTTATTT TTCTAAATAC ATTCAAATAT GTATCCGCTC ATGAGACAAT
      301 AACCCTGATA AATGCTTCAA TAATATTGAA AAAGGAAGAC TATGAGTATT CAACATTTCC
      361 GTGTCTGCTT ACATAAACAG TAATACAAGG GGTGTTTACT AGAGGTTGAT CGGGCACGTA
      421 AGAGGTTCCA ACTTTCACCA TAATGAAATA AGATCACTAC CGGGCGTATT TTTTGAGTTA
      481 TCGAGATTTT CAGGAGCTAA GGAAGCTAAA ATGGAGAAAA AAATCACGGG ATATACCACC
      541 GTTGATATAT CCCAATGGCA TCGTAAAGAA CATTTTGAGG CATTTCAGTC AGTTGCTCAA
      601 TGTACCTATA ACCAGACCGT TCAGCTGGAT ATTACGGCCT TTTTAAAGAC CGTAAAGAAA
      661 AATAAGCACA AGTTTTATCC GGCCTTTATT CACATTCTTG CCCGCCTGAT GAACGCTCAC
      721 CCGGAGTTTC GTATGGCCAT GAAAGACGGT GAGCTGGTGA TCTGGGATAG TGTTCACCCT
      781 TGTTACACCG TTTTCCATGA GCAAACTGAA ACGTTTTCGT CCCTCTGGAG TGAATACCAC
      841 GACGATTTCC GGCAGTTTCT CCACATATAT TCGCAAGATG TGGCGTGTTA CGGTGAAAAC
      901 CTGGCCTATT TCCCTAAAGG GTTTATTGAG AATATGTTTT TTGTCTCAGC CAATCCCTGG
      961 GTGAGTTTCA CCAGTTTTGA TTTAAACGTG GCCAATATGG ACAACTTCTT CGCCCCCGTT
     1021 TTCACGATGG GCAAATATTA TACGCAAGGC GACAAGGTGC TGATGCCGCT GGCGATCCAG
     1081 GTTCATCATG CCGTTTGTGA TGGCTTCCAT GTCGGCCGCA TGCTTAATGA ATTACAACAG
     1141 TACTGTGATG AGTGGCAGGG CGGGGCGTAA TAATACTAGC TCCGGCAAAA AAACGGGCAA
     1201 GGTGTCACCA CCCTGCCCTT TTTCTTTAAA ACCGAAAAGA TTACTTCGCG TTTGCCACCT
     1261 GACGTCTAAG CTGTCAGACC AAGTTTACTC ATATATACTT TAGATTGATT TACGCGCCCT
     1321 GTAGCGGCGC ATTAAGCGCG GCGGGTGTGG TGGTTACGCG CAGCGTGACC GCTACACTTG
     1381 CCAGCGCCCT AGCGCCCGCT CCTTTCGCTT TCTTCCCTTC CTTTCTCGCC ACGTTCGCCG
     1441 GCTTTCCCCG TCAAGCTCTA AATCGGGGGC TCCCTTTAGG GTTCCGATTT AGTGCTTTAC
     1501 GGCACCTCGA CCCCAAAAAA CTTGATTTGG GTGATGGTTC ACGTAGTGGG CCATCGCCCT
     1561 GATAGACGGT TTTTCGCCCT TTGACGTTGG AGTCCACGTT CTTTAATAGT GGACTCTTGT
     1621 TCCAAACTTG AACAACACTC AACCCTATCT CGGGCTATTC TTTTGATTTA TAAGGGATTT
     1681 TGCCGATTTC GGCCTATTGG TTAAAAAATG AGCTGATTTA ACAAAAATTT AACGCGAATT
     1741 TTAACAAAAT ATTAACGTTT ACAATTTAAA AGGATCTAGG TGAAGATCCT TTTTGATAAT
     1801 CTCATGACCA AAATCCCTTA ACGTGAGTTT TCGTTCCACT GAGCGTCAGA CCCCGTAGAA
     1861 AAGATCAAAG GATCTTCTTG AGATCCTTTT TTTCTGCGCG TAATCTGCTG CTTGCAAACA
     1921 AAAAAACCAC CGCTACCAGC GGTGGTTTGT TTGCCGGATC AAGAGCTACC AACTCTTTTT
     1981 CCGAAGGTAA CTGGCTTCAG CAGAGCGCAG ATACCAAATA CTGTCCTTCT AGTGTAGCCG
     2041 TAGTTAGGCC ACCACTTCAA GAACTCTGTA GCACCGCCTA CATACCTCGC TCTGCTAATC
     2101 CTGTTACCAG TCAGGCATTT GAGAAGCACA CGGTCACACT GCTTCCGGTA GTCAATAAAC
     2161 CGGTAAACCA GCAATAGACA TAAGCGGCTA TTTAACGACC CTGCCCTGAA CCGACGACCG
     2221 GGTCGAATTT GCTTTCGAAT TTCTGCCATT CATCCGCTTA TTATCACTTA TTCAGGCGTA
     2281 GCACCAGGCG TTTAAGGGCA CCAATAACTG CCTTAAAAAA AGATAGCGGA GTGTATACTG
     2341 GCTTACTATG TTGGCACTGA TGAGGGTGTC AGTGAAGTGC TTCATGTGGC AGGAGAAAAA
     2401 AGGCTGCACC GGTGCGTCAG CAGAATATGT GATACAGGAT ATATTCCGCT TCCTCGCTCA
     2461 CTGACTCGCT ACGCTCGGTC GTTCGACTGC GGCGAGCGGA AATGGCTTAC GAACGGGGCG
     2521 GAGATTTCCT GGAAGATGCC AGGAAGATAC TTAACAGGGA AGTGAGAGGG CCGCGGCAAA
     2581 GCCGTTTTTC CATAGGCTCC GCCCCCCTGA CAAGCATCAC GAAATCTGAC GCTCAAATCA
     2641 GTGGTGGCGA AACCCGACAG GACTATAAAG ATACCAGGCG TTTCCCCCTG GCGGCTCCCT
     2701 CGTGCGCTCT CCTGTTCCTG CCTTTCGGTT TACCGGTGTC ATTCCGCTGT TATGGCCGCG
     2761 TTTGTCTCAT TCCACGCCTG ACACTCAGTT CCGGGTAGGC AGTTCGCTCC AAGCTGGACT
     2821 GTATGCACGA ACCCCCCGTT CAGTCCGACC GCTGCGCCTT ATCCGGTAAC TATCGTCTTG
     2881 AGTCCAACCC GGAAAGACAT GCAAAAGCAC CACTGGCAGC AGCCACTGGT AATTGATTTA
     2941 GAGGAGTTAG TCTTGAAGTC ATGCGCCGGT TAAGGCTAAA CTGAAAGGAC AAGTTTTGGT
     3001 GACTGCGCTC CTCCAAGCCA GTTACCTCGG TTCAAAGAGT TGGTAGCTCA GAGAACCTTC
     3061 GAAAAACCGC CCTGCAAGGC GGTTTTTTCG TTTTCAGAGC AAGAGATTAC GCGCAGACCA
     3121 AAACGATCTC AAGAAGATCA TCTTATTAAT CAGATAAAAT ATTTGCTCAT GAGCCCGAAG
     3181 TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT
     3241 GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCTGCTC ATGTTTGACA
     3301 GCTTATCATC GATGCATaat gtgcctgtca aatggacgaa gcagggattc tgcaaaccct
     3361 atgctactcc gtcaagccgt caattgtctg attcgttacc aattatgaca acttgacggc
     3421 tacatcattc actttttctt cacaaccggc acggaactcg ctcgggctgg ccccggtgca
     3481 ttttttaaat acccgcgaga aatagagttg atcgtcaaaa ccaacattgc gaccgacggt
     3541 ggcgataggc atccgggtgg tgctcaaaag cagcttcgcc tggctgatac gttggtcctc
     3601 gcgccagctt aagacgctaa tccctaactg ctggcggaaa agatgtgaca gacgcgacgg
     3661 cgacaagcaa acatgctgtg cgacgctggc gatatcaaaa ttgctgtctg ccaggtgatc
     3721 gctgatgtac tgacaagcct cgcgtacccg attatccatc ggtggatgga gcgactcgtt
     3781 aatcgcttcc atgcgccgca gtaacaattg ctcaagcaga tttatcgcca gcagctccga
     3841 atagcgccct tccccttgcc cggcgttaat gatttgccca aacaggtcgc tgaaatgcgg
     3901 ctggtgcgct tcatccgggc gaaagaaccc cgtattggca aatattgacg gccagttaag
     3961 ccattcatgc cagtaggcgc gcggacgaaa gtaaacccac tggtgatacc attcgcgagc
     4021 ctccggatga cgaccgtagt gatgaatctc tcctggcggg aacagcaaaa tatcacccgg
     4081 tcggcaaaca aattctcgtc cctgattttt caccaccccc tgaccgcgaa tggtgagatt
     4141 gagaatataa cctttcattc ccagcggtcg gtcgataaaa aaatcgagat aaccgttggc
     4201 ctcaatcggc gttaaacccg ccaccagatg ggcattaaac gagtatcccg gcagcagggg
     4261 atcattttgc gcttcagcca tacttttcat actcccgcca ttcagagaag aaaccaattg
     4321 tccatattgc atcagacatt gccgtcactg cgtcttttac tggctcttct cgctaaccaa
     4381 accggtaacc ccgcttatta aaagcattct gtaacaaagc gggaccaaag ccatgacaaa
     4441 aacgcgtaac aaaagtgtct ataatcacgg cagaaaagtc cacattgatt atttgcacgg
     4501 cgtcacactt tgctatgcca tagcattttt atccataaga ttagcggatc ctacctgacg
     4561 ctttttatcg caactctcta ctgtttctcc atacccgttt ttttgggcta acaggaggaa
     4621 ttaaccATGG GGGGTTCTCA TCATCATCAT CATCATGGTA TGGCTAGCGC CGGCATGGTG
     4681 AGCAAGGGCG AGGAGCTGTT CACCGGGGTG GTGCCCATCC TGGTCGAGCT GGACGGCGAC
     4741 GTAAACGGCC ACAAGTTCAG CGTGTCCGGC GAGGGCGAGG GCGATGCCAC CTACGGCAAG
     4801 CTGACCCTGA AGCTCATCTG CACCACCGGC AAGCTGCCCG TGCCCTGGCC CACCCTCGTG
     4861 ACCACCCTCG GCTACGGCCT GCAGTGCTTC GCCCGCTACC CCGACCACAT GAAGCAGCAC
     4921 GACTTCTTCA AGTCCGCCAT GCCCGAAGGC TACGTCCAGG AGCGCACCAT CTTCTTCAAG
     4981 GACGACGGCA ACTACAAGAC CCGCGCCGAG GTGAAGTTCG AGGGCGACAC CCTGGTGAAC
     5041 CGCATCGAGC TGAAGGGCAT CGACTTCAAG GAGGACGGCA ACATCCTGGG GCACAAGCTG
     5101 GAGTACAACT ACAACAGCCA CAACGCCTAT ATCACCGCCG GCTCTGGCTC TGGTAGCGGt
     5161 CTAGAGGGTA GTGGCCAGCT GGAACAAGAG TTGGCTGCGC TGGACCAACA GATTGCCGCG
     5221 CTGAAACAGC GTCGCGCAGC TCTGAAATGG CAGATCCAAG GTTAGgtacc aagcttggct
     5281 gttttggcgg atgagagaag attttcagcc tgatacagat taaatcagaa cgcagaagcg
     5341 gtctgataaa acagaatttg cctggcggca gtagcgcggt ggtcccacct gaccccatgc
     5401 cgaactcaga agtgaaacgc cgtagcgccg atggtagtgt ggggtctccc cat
//