LOCUS       pBAD-6H-CC-Di-(12AA_Lin 4425 bp ds-DNA     circular     14-APR-2021
DEFINITION  .
KEYWORDS    "marker:AmpR"                                                      
FEATURES             Location/Qualifiers
     promoter        540..824
                     /label="araBAD promoter"
                     /ApEinfo_revcolor=#c6c9d1
                     /ApEinfo_fwdcolor=#c6c9d1
     CDS             859..1314
                     /label="Translation 859-1314"
     CDS             871..888
                     /label="6xHis"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     misc_feature    907..996
                     /label="CC-Di"
                     /ApEinfo_revcolor=#f8d3a9
                     /ApEinfo_fwdcolor=#f8d3a9
     CDS             1027..1027
                     /label="SUMO"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     misc_feature    1030..1030
                     /label="Split Site"
                     /ApEinfo_revcolor=#c7b0e3
                     /ApEinfo_fwdcolor=#c7b0e3
     misc_feature    1030..1281
                     /label="C-Venus"
                     /ApEinfo_revcolor=#b4abac
                     /ApEinfo_fwdcolor=#b4abac
     misc_feature    1282..1299
                     /label="GSGSGS Linker"
                     /ApEinfo_revcolor=#84b0dc
                     /ApEinfo_fwdcolor=#84b0dc
     misc_feature    1282..1314
                     /label="12 AA Linker"
                     /ApEinfo_revcolor=#b1ff67
                     /ApEinfo_fwdcolor=#b1ff67
     CDS             1315..1327
                     /label="Translation 1315-1327"
     terminator      1534..1620
                     /label="rrnB T1 terminator"
                     /ApEinfo_revcolor=#c6c9d1
                     /ApEinfo_fwdcolor=#c6c9d1
     terminator      1712..1739
                     /label="rrnB T2 terminator"
                     /ApEinfo_revcolor=#c6c9d1
                     /ApEinfo_fwdcolor=#c6c9d1
     promoter        1759..1850
                     /label="AmpR promoter"
                     /ApEinfo_revcolor=#c6c9d1
                     /ApEinfo_fwdcolor=#c6c9d1
     CDS             1851..1919
                     /label="AmpR"
                     /ApEinfo_revcolor=#b7e6d7
                     /ApEinfo_fwdcolor=#b7e6d7
     CDS             1920..2711
                     /label="AmpR"
                     /ApEinfo_revcolor=#b7e6d7
                     /ApEinfo_fwdcolor=#b7e6d7
     rep_origin      2882..3470
                     /label="ori"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     misc_feature    3656..3796
                     /label="bom"
                     /ApEinfo_revcolor=#b4abac
                     /ApEinfo_fwdcolor=#b4abac
     CDS             complement(4060..513)
                     /label="araC"
                     /ApEinfo_revcolor=#993366
                     /ApEinfo_fwdcolor=#993366
ORIGIN
        1 gagcgactcg ttaatcgctt ccatgcgccg cagtaacaat tgctcaagca gatttatcgc
       61 cagcagctcc gaatagcgcc cttccccttg cccggcgtta atgatttgcc caaacaggtc
      121 gctgaaatgc ggctggtgcg cttcatccgg gcgaaagaac cccgtattgg caaatattga
      181 cggccagtta agccattcat gccagtaggc gcgcggacga aagtaaaccc actggtgata
      241 ccattcgcga gcctccggat gacgaccgta gtgatgaatc tctcctggcg ggaacagcaa
      301 aatatcaccc ggtcggcaaa caaattctcg tccctgattt ttcaccaccc cctgaccgcg
      361 aatggtgaga ttgagaatat aacctttcat tcccagcggt cggtcgataa aaaaatcgag
      421 ataaccgttg gcctcaatcg gcgttaaacc cgccaccaga tgggcattaa acgagtatcc
      481 cggcagcagg ggatcatttt gcgcttcagc catacttttc atactcccgc cattcagaga
      541 agaaaccaat tgtccatatt gcatcagaca ttgccgtcac tgcgtctttt actggctctt
      601 ctcgctaacc aaaccggtaa ccccgcttat taaaagcatt ctgtaacaaa gcgggaccaa
      661 agccatgaca aaaacgcgta acaaaagtgt ctataatcac ggcagaaaag tccacattga
      721 ttatttgcac ggcgtcacac tttgctatgc catagcattt ttatccataa gattagcgga
      781 tcctacctga cgctttttat cgcaactctc tactgtttct ccatacccgt ttttttgggc
      841 taacaggagg aattaaccat ggggggttct catcatcatc atcatcatgg tatggctagc
      901 CTGCAGGGCg aaattgctgc gctgaaacag gaaatcgctg ccctgaaaaa agagaatgcc
      961 gctctgaaat gggaaatcgc agcgctgaaa caaGGTAGCG GTAGCGGCAG CGGtgagGGC
     1021 CTGCAGGGGG ACAAGCAGAA GAACGGCATC AAGGCCAACT TCAAGATCCG CCACAACATC
     1081 GAGGACGGCG GCGTGCAGCT CGCCGACCAC TACCAGCAGA ACACCCCCAT CGGCGACGGC
     1141 CCCGTGCTGC TGCCCGACAA CCACTACCTG AGCTACCAGT CCAAGCTGAG CAAAGACCCC
     1201 AACGAGAAGC GCGATCACAT GGTCCTGCTG GAGTTCGTGA CCGCCGCCGG GATCACTCTC
     1261 GGCATGGACG AGCTGTACAA GGGCAGCGGC AGCGGCAGCG Gtctagaggg tagtGGCTGA
     1321 GGTACCAAGC TTGGCTGTTT GGCGGATGAG AGAAGATTtt cagcctgata cagattaaat
     1381 cagaacgcag aagcggtctg ataaaacaga atttgcctgg cggcagtagc gcggtggtcc
     1441 cacctgaccc catgccgaac tcagaagtga aacgccgtag cgccgatggt agtgtggggt
     1501 ctccccatgc gagagtaggg aactgccagg catcaaataa aacgaaaggc tcagtcgaaa
     1561 gactgggcct ttcgttttat ctgttgtttg tcggtgaacg ctctcctgag taggacaaat
     1621 ccgccgggag cggatttgaa cgttgcgaag caacggcccg gagggtggcg ggcaggacgc
     1681 ccgccataaa ctgccaggca tcaaattaag cagaaggcca tcctgacgga tggccttttt
     1741 gcgtttctac aaactctttt tgtttatttt tctaaataca ttcaaatatg tatccgctca
     1801 tgagacaata accctgataa atgcttcaat aatattgaaa aaggaagagt atgagtattc
     1861 aacatttccg tgtcgccctt attccctttt ttgcggcatt ttgccttcct gtttttgctc
     1921 acccagaaac gctggtgaaa gtaaaagatg ctgaagatca gttgggtgca cgagtgggtt
     1981 acatcgaact ggatctcaac agcggtaaga tccttgagag ttttcgcccc gaagaacgtt
     2041 ttccaatgat gagcactttt aaagttctgc tatgtggcgc ggtattatcc cgtgttgacg
     2101 ccgggcaaga gcaactcggt cgccgcatac actattctca gaatgacttg gttgagtact
     2161 caccagtcac agaaaagcat cttacggatg gcatgacagt aagagaatta tgcagtgctg
     2221 ccataaccat gagtgataac actgcggcca acttacttct gacaacgatc ggaggaccga
     2281 aggagctaac cgcttttttg cacaacatgg gggatcatgt aactcgcctt gatcgttggg
     2341 aaccggagct gaatgaagcc ataccaaacg acgagcgtga caccacgatg cctgtagcaa
     2401 tggcaacaac gttgcgcaaa ctattaactg gcgaactact tactctagct tcccggcaac
     2461 aattaataga ctggatggag gcggataaag ttgcaggacc acttctgcgc tcggcccttc
     2521 cggctggctg gtttattgct gataaatctg gagccggtga gcgtgggtct cgcggtatca
     2581 ttgcagcact ggggccagat ggtaagccct cccgtatcgt agttatctac acgacgggga
     2641 gtcaggcaac tatggatgaa cgaaatagac agatcgctga gataggtgcc tcactgatta
     2701 agcattggta actgtcagac caagtttact catatatact ttagattgat ttaaaacttc
     2761 atttttaatt taaaaggatc taggtgaaga tcctttttga taatctcatg accaaaatcc
     2821 cttaacgtga gttttcgttc cactgagcgt cagaccccgt agaaaagatc aaaggatctt
     2881 cttgagatcc tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac
     2941 cagcggtggt ttgtttgccg gatcaagagc taccaactct ttttccgaag gtaactggct
     3001 tcagcagagc gcagatacca aatactgtcc ttctagtgta gccgtagtta ggccaccact
     3061 tcaagaactc tgtagcaccg cctacatacc tcgctctgct aatcctgtta ccagtggctg
     3121 ctgccagtgg cgataagtcg tgtcttaccg ggttggactc aagacgatag ttaccggata
     3181 aggcgcagcg gtcgggctga acggggggtt cgtgcacaca gcccagcttg gagcgaacga
     3241 cctacaccga actgagatac ctacagcgtg agctatgaga aagcgccacg cttcccgaag
     3301 ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg
     3361 agcttccagg gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc cacctctgac
     3421 ttgagcgtcg atttttgtga tgctcgtcag gggggcggag cctatggaaa aacgccagca
     3481 acgcggcctt tttacggttc ctggcctttt gctggccttt tgctcacatg ttctttcctg
     3541 cgttatcccc tgattctgtg gataaccgta ttaccgcctt tgagtgagct gataccgctc
     3601 gccgcagccg aacgaccgag cgcagcgagt cagtgagcga ggaagcggaa gagcgcctga
     3661 tgcggtattt tctccttacg catctgtgcg gtatttcaca ccgcatatgg tgcactctca
     3721 gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga
     3781 ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg
     3841 tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca
     3901 gaggttttca ccgtcatcac cgaaacgcgc gaggcagcag atcaattcgc gcgcgaaggc
     3961 gaagcggcat gcataatgtg cctgtcaaat ggacgaagca gggattctgc aaaccctatg
     4021 ctactccgtc aagccgtcaa ttgtctgatt cgttaccaat tatgacaact tgacggctac
     4081 atcattcact ttttcttcac aaccggcacg gaactcgctc gggctggccc cggtgcattt
     4141 tttaaatacc cgcgagaaat agagttgatc gtcaaaacca acattgcgac cgacggtggc
     4201 gataggcatc cgggtggtgc tcaaaagcag cttcgcctgg ctgatacgtt ggtcctcgcg
     4261 ccagcttaag acgctaatcc ctaactgctg gcggaaaaga tgtgacagac gcgacggcga
     4321 caagcaaaca tgctgtgcga cgctggcgat atcaaaattg ctgtctgcca ggtgatcgct
     4381 gatgtactga caagcctcgc gtacccgatt atccatcggt ggatg
//