LOCUS       Exported               13542 bp ds-DNA     circular SYN 04-FEB-2019
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 13542)
  AUTHORS   Dr. Michael Ward
  TITLE     Direct Submission
  JOURNAL   Exported Monday, Sep 19, 2022 from SnapGene 5.0.8
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..13542
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     misc_feature    413..568
                     /label=AAV3'
     misc_feature    569..1364
                     /label=C13-RA
     misc_feature    1386..1761
                     /label=TRE3G
     protein_bind    1391..1409
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    1427..1445
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    1463..1481
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    1499..1517
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    1535..1553
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    1571..1589
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    1607..1625
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     misc_feature    1769..5274
                     /label=genewiz insert SPI1-CEBP-IRF5
     CDS             1783..5268
                     /codon_start=1
                     /label=SPI1-CEBP-IRF5
                     /translation="MEGFPLVPPPSEDLVPYDTDLYQRQTHEYYPYLSSDGESHSDHYW
                     DFHPHHVHSEFESFAENNFTELQSVQPPQLQQLYRHMELEQMHVLDTPMVPPHPSLGHQ
                     VSYLPRMCLQYPSLSPAQPSSDEEEGERQSPPLEVSDGEADGLEPGPGLLPGETGSKKK
                     IRLYQFLLDLLRSGDMKDSIWWVDKDKGTFQFSSKHKEALAHRWGIQKGNRKKMTYQKM
                     ARALRNYGKTGEVKKVKKKLTYQFSGEVLGRGGLAERRHPPHSRMHSRGSGEGRGSLLT
                     CGDVEENPGPMQRLVAWDPACLPLPPPPPAFKSMEVANFYYEADCLAAAYGGKAAPAAP
                     PAARPGPRPPAGELGSIGDHERAIDFSPYLEPLGAPQAPAPATATDTFEAAPPAPAPAP
                     ASSGQHHDFLSDLFSDDYGGKNCKKPAEYGYVSLGRLGAAKGALHPGCFAPLHPPPPPP
                     PPPAELKAEPGFEPADCKRKEEAGAPGGGAGMAAGFPYALRAYLGYQAVPSGSSGSLST
                     SSSSSPPGTPSPADAKAPPTACYAGAAPAPSQVKSKAKKTVDKHSDEYKIRRERNNIAV
                     RKSRDKAKMRNLETQHKVLELTAENERLQKKVEQLSRELSTLRNLFKQLPEPLLASSGH
                     CGSMHSRGSGEGRGSLLTCGDVEENPGPMNQSIPVAPTPPRRVRLKPWLVAQVNSCQYP
                     GLQWVNGEKKLFCIPWRHATRHGPSQDGDNTIFKAWAKETGKYTEGVDEADPAKWKANL
                     RCALNKSRDFRLIYDGPRDMPPQPYKIYEVCSNGPAPTDSQPPEDYSFGAGEEEEEEEE
                     LQRMLPSLSLTEDVKWPPTLQPPTLRPPTLQPPTLQPPVVLGPPAPDPSPLAPPPGNPA
                     GFRELLSEVLEPGPLPASLPPAGEQLLPDLLISPHMLPLTDLEIKFQYRGRPPRALTIS
                     NPHGCRLFYSQLEATQEQVELFGPISLEQVRFPSPEDIPSDKQRFYTNQLLDVLDRGLI
                     LQLQGQDLYAIRLCQCKVFWSGPCASAHDSCPNPIQREVKTKLFSLEHFLNELILFQKG
                     QTNTPPPFEIFFCFGEEWPDRKPREKKLITVQVVPVAARLLLEMFSGELSWSADSIRLQ
                     ISNPDLKDRMVEQFKELHHIWQSQQRLQPVAQAPPGAGLGVGQGPWPMHPAGMQ"
     CDS             2602..2655
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             3718..3771
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     polyA_signal    5285..5406
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5455..5471)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    5479..5495
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(5503..5533)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    5548..5569
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     misc_feature    5759..6213
                     /label=RBGpolyA (minus last 72bp)
     CDS             complement(6223..6969)
                     /codon_start=1
                     /label=rtTA3G
                     /translation="MSRLDKSKVINSALELLNGVGIEGLTTRKLAQKLGVEQPTLYWHV
                     KNKRALLDALPIEMLDRHHTHSCPLEGESWQDFLRNNAKSYRCALLSHRDGAKVHLGTR
                     PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEEQEHQVAKEERETPTT
                     DSMPPLLKQAIELFDRQGAEPAFLFGLELIICGLEKQLKCESGGPTDALDDFDLDMLPA
                     DALDDFDLDMLPADALDDFDLDMLPG"
     misc_feature    complement(7077..8705)
                     /label=CAG
     polyA_signal    8711..8935
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     protein_bind    complement(8957..8990)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (GCATACAT)."
     CDS             complement(8997..9698)
                     /codon_start=1
                     /label=mCherry
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDE"
     misc_feature    complement(9721..10263)
                     /label=EF-1alpha promoter
     polyA_signal    10270..10494
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     CDS             complement(10499..11290)
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="IEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRPV
                     LFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLSS
                     HLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQG
                     LAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIAL
                     ATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             complement(11294..11347)
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     misc_feature    11371..11396
                     /label=SA
                     /note="splice acceptor site"
     protein_bind    complement(11397..11430)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (GCATACAT)."
     misc_feature    11439..12270
                     /label=C13-LA
     CDS             12288..13148
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      join(13319..13542,1..365)
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac
       61 gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca
      121 gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg
      181 ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag
      241 gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt
      301 ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat
      361 ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc gaattcgccc ttagagcaga
      421 gccaggaacc cctgtaggga aggggcagga gagccagggg catgagatgg tggacgagga
      481 agggggacag ggaagcctga gcgcctctcc tgggcttgcc aaggactcaa acccagaagc
      541 ccagagcagg gccttaggga agcgggacgg ctctgttgga gagtccagta ttgaattagt
      601 gatagataca cagaaaaaaa gcaaattgaa taggtgaatg gataaacaaa ctgtggaaca
      661 tccagacaat gaaatattca ccaataaaaa gaaatgagct atcaagccac attggctatg
      721 cccttcacat ttaaggagga accttaaatg catattgcca aattttaaaa gctagtctga
      781 aaatgctaca gactgtatga ttccaattat atgacatttt agaaaaggca aaactataga
      841 gatcagtggt tgccagcggc tcatggaggc aggatgaatt ggtggaacac agggatgctt
      901 tcagggtaat gaaaatatct tgtgtgatcc tgtaataatg gatacgtggc ataatgcatt
      961 tgttaaaacc tgtaaactag ccaggtatag tggctttttt gcacctatag tctcagctac
     1021 ttggtaggct gagacaggaa gatcacttga gcccaggact tcaagaccag cctgggtaat
     1081 atagtaagac cccatctcta ttttttaaag aaattaagaa agaaaagaaa cccatcaact
     1141 gtataacaca aagaatcgta atgtaaacta cagtcctgag taatgaaaca tttttaaaga
     1201 aataaataaa ttcaaggcaa ttattaactt caggaaaaac agtaaagtaa acataagtat
     1261 acaaatcaaa catggctcag ttgtgaaaat atttacaagg tcactttaag taacattgaa
     1321 tgttgatata atcccaaaat atatttatgt tggaaggatg aggagatatc tccaccccac
     1381 agtggtttac tccctatcag tgatagagaa cgtatgaaga gtttactccc tatcagtgat
     1441 agagaacgta tgcagacttt actccctatc agtgatagag aacgtataag gagtttactc
     1501 cctatcagtg atagagaacg tatgaccagt ttactcccta tcagtgatag agaacgtatc
     1561 tacagtttac tccctatcag tgatagagaa cgtatatcca gtttactccc tatcagtgat
     1621 agagaacgta taagctttag gcgtgtacgg tgggcgccta taaaagcaga gctcgtttag
     1681 tgaaccgtca gatcgcctgg agcaattcca caacactttt gtcttatacc aactttccgt
     1741 accacttcct accctcgtaa acttaaggtt aattaagcca ccatggaagg attccccctg
     1801 gtgccccctc cctccgaaga tctcgtgccc tacgacaccg atctgtacca gagacagaca
     1861 catgagtatt acccctacct gtccagcgac ggagagtccc acagcgacca ctattgggat
     1921 tttcaccccc accatgtgca ctccgagttc gagagctttg ctgagaacaa cttcaccgag
     1981 ctccagtccg tgcagcctcc tcaactccag cagctctata gacacatgga gctggagcag
     2041 atgcatgtgc tggatacccc catggtgcct ccccacccta gcctgggaca ccaagtctcc
     2101 tacctgccca ggatgtgcct ccagtaccct tccctgtccc ccgctcagcc cagcagcgat
     2161 gaggaagaag gcgaaaggca gtcccctcct ctggaggtgt ccgacggaga ggctgatggc
     2221 ctcgagcctg gacccggact gctgcccggc gagaccggca gcaaaaagaa gattaggctg
     2281 taccagttcc tcctggatct gctcagatcc ggcgacatga aggacagcat ttggtgggtc
     2341 gacaaggaca agggcacctt tcagttctcc agcaagcaca aagaggctct ggctcacagg
     2401 tggggcatcc agaagggcaa caggaagaag atgacctatc agaagatggc cagggccctc
     2461 agaaactacg gcaagaccgg agaggtgaaa aaagtcaaga agaagctcac ataccaattc
     2521 tccggcgagg tgctgggaag aggaggactg gctgagagga gacaccctcc tcactccagg
     2581 atgcatagca ggggcagcgg cgagggaagg ggttctctgc tcacctgtgg agatgtcgag
     2641 gagaaccccg gccccatgca gaggctggtg gcttgggacc ctgcctgcct ccctctccct
     2701 cctccccctc ctgctttcaa gagcatggag gtcgccaact tttactacga ggccgattgt
     2761 ctggccgccg cttatggcgg aaaagctgcc cccgccgccc cccccgccgc cagacccggc
     2821 cccaggcccc ccgctggcga actgggaagc atcggcgacc acgagagggc catcgacttt
     2881 agcccctacc tggagcctct cggcgccccc caagcccccg cccctgccac agctaccgac
     2941 acattcgagg ccgccccccc tgcccccgct cccgcccccg cctccagcgg ccagcaccat
     3001 gactttctgt ccgacctgtt ctccgatgac tacggcggca aaaactgcaa gaaacccgcc
     3061 gagtacggct atgtgtccct gggcaggctg ggagccgcta aaggagccct gcaccctggc
     3121 tgttttgccc ctctgcaccc tcctcctccc cccccccccc cccccgccga gctcaaagct
     3181 gagcctggct tcgagcccgc cgattgcaag agaaaagagg aggctggcgc ccctggagga
     3241 ggcgccggca tggctgctgg attcccctat gctctgaggg cctacctggg ctaccaagcc
     3301 gtccctagcg gaagcagcgg ctccctgtcc acatcctcct cctccagccc tcctggaaca
     3361 ccttcccccg ccgatgctaa agcccccccc acagcctgtt acgccggcgc cgctcccgcc
     3421 ccttcccaag tgaaaagcaa ggccaagaag accgtggaca agcactccga cgagtataaa
     3481 atcagaaggg aaagaaacaa catcgccgtc agaaagtcca gggataaggc caagatgagg
     3541 aacctcgaga cacagcacaa ggtgctcgag ctcaccgccg agaacgaaag actgcaaaag
     3601 aaagtcgagc agctctccag ggaactgtcc accctcagaa acctgttcaa acagctgccc
     3661 gagcccctcc tggccagcag cggccactgt ggaagcatgc attccagagg ttctggcgaa
     3721 ggaaggggca gcctgctgac atgcggcgac gtggaagaaa atcccggccc catgaatcag
     3781 agcattcccg tggcccctac cccccctaga agagtcagac tgaaaccttg gctcgtcgcc
     3841 caggtgaata gctgtcagta ccctggcctg caatgggtga acggagagaa gaagctcttt
     3901 tgcatcccct ggagacacgc taccaggcac ggaccttccc aggatggcga caacaccatt
     3961 ttcaaggcct gggccaagga gaccggaaaa tacaccgagg gcgtcgacga agccgaccct
     4021 gccaagtgga aagccaacct cagatgtgcc ctcaacaaga gcagggattt caggctgatt
     4081 tacgacggcc ccagggacat gcctcctcaa ccttacaaaa tctatgaggt gtgcagcaat
     4141 ggccctgccc ccacagacag ccagcctccc gaggactatt ccttcggagc cggagaagag
     4201 gaagaagaag aagaggagct ccagaggatg ctccccagcc tgagcctcac cgaagacgtg
     4261 aagtggcccc ctacactcca gcctcccaca ctgaggcccc ccacactcca accccctaca
     4321 ctgcagcccc ccgtggtgct gggccccccc gcccccgacc ccagccctct ggctcctcct
     4381 cccggcaatc ctgccggctt tagggaactg ctgagcgaag tcctggagcc cggccccctg
     4441 cctgctagcc tgccccctgc tggagagcag ctgctgcctg atctcctcat tagcccccac
     4501 atgctgcccc tcaccgatct ggagatcaag ttccagtaca gaggcaggcc ccccagagcc
     4561 ctgaccatct ccaaccctca cggctgtagg ctcttctact cccagctgga ggccacacaa
     4621 gagcaggtgg aactgttcgg ccccatcagc ctggaacagg tgaggttccc ctcccccgag
     4681 gatatcccct ccgacaagca gaggttttac accaatcagc tcctggatgt gctggacaga
     4741 ggcctgatcc tgcagctgca gggccaggat ctctacgcta tcaggctctg ccagtgcaag
     4801 gtgttttgga gcggaccttg tgctagcgcc cacgactcct gtcccaaccc cattcaaaga
     4861 gaggtgaaga ccaaactctt cagcctcgag cacttcctca atgagctcat cctgttccag
     4921 aaaggccaaa ccaatacccc cccccctttc gagatcttct tctgcttcgg agaggagtgg
     4981 cctgacagga agcccagaga aaaaaagctc attacagtgc aggtcgtgcc cgtcgctgcc
     5041 agactcctcc tggagatgtt tagcggcgag ctcagctgga gcgccgacag catcaggctg
     5101 cagatcagca accccgacct caaagatagg atggtggagc agtttaaaga gctccaccac
     5161 atctggcaat cccagcagag actgcagcct gtcgctcagg ctccccccgg cgccggcctg
     5221 ggcgtgggcc aaggcccctg gcctatgcac cctgctggaa tgcagtgaat cgatcgccca
     5281 ccccaacttg tttattgcag cttataatgg ttacaaataa agcaatagca tcacaaattt
     5341 cacaaataaa gcattttttt cactgcattc tagttgtggt ttgtccaaac tcatcaatgt
     5401 atcttatcat gtctgtatac cgtcgacctc tagctagagc ttggcgtaat catggtcata
     5461 gctgtttcct gtgtgaaatt gttatccgct cacaattcca cacaacatac gagccggaag
     5521 cataaagtgt aaagcctggg gtgcctaatg agtgagctaa ctcacattaa ttgcgttgcg
     5581 ctcactgccc gctttccagt cgggaaacct gtcgtgccag ctgcattaat gaatcggcca
     5641 acgcgcgggg agaggcggtt tgcgtattgg gcgctcttcc gcttcctcgc tcactgactc
     5701 gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg acggccgcac
     5761 atgtaaggaa aattttaggg atgttaaaga aaaaaataac acaaaacaaa atataaaaaa
     5821 aatctaacct caagtcaagg cttttctatg gaataaggaa tggacagcag ggggctgttt
     5881 catatactga tgacctcttt atagccaacc tttgttcatg gcagccagca tatgggcata
     5941 tgttgccaaa ctctaaacca aatactcatt ctgatgtttt aaatgatttg ccctcccata
     6001 tgtccttccg agtgagagac acaaaaaatt ccaacacact attgcaatga aaataaattt
     6061 cctttattag ccagaagtca gatgctcaag gggcttcatg atgtccccat aatttttggc
     6121 agagggaaaa agatctcagt ggtatttgtg agccagggca ttggccacac cagccaccac
     6181 cttctgatag gcagcctgca cctgaggagt gaattcacgc gtttagcctg gcagcatatc
     6241 gagatcgaag tcatcgagag cgtcagcagg gagcatatcc agatcaaaat catccagggc
     6301 atcggcgggc agcatgtcca ggtcgaagtc gtccagggcg tcggtgggtc cgccgctctc
     6361 gcacttcagc tgcttctcga ggccgcagat gatcagttcc aggccgaaca ggaaggcggg
     6421 ctcggcgccc tgtctgtcga acagctcgat ggcctgcttc agcagggggg gcatgctgtc
     6481 ggtggtgggt gtctctcttt cctctttggc gacctggtgt tcctgttctt ccagcacgca
     6541 gcccagggtg aagtggccca cggcgctcag ggcgtacagg gcgttttcca ggctgaagcc
     6601 ctgctggcac aggaaggcca gctggttttc cagtgtctcg tactgcttct cggtgggtct
     6661 ggtgcccagg tgcactttgg cgccgtcccg gtggctcagc agggcgcatc tgtagctctt
     6721 ggcgttgttc cgcaggaagt cctgccagct ctcgccttcc agagggcagc tgtgggtgtg
     6781 gtgccggtcc agcatctcga tgggcagggc gtccagcagg gcccgcttgt tcttcacgtg
     6841 ccagtacagg gtgggctgtt ccacgcccag tttctgggcc agcttccggg tggtcaggcc
     6901 ctcgatgccc acgccgttca gcagttccag ggcgctgttg atcactttgc tcttgtccag
     6961 ccggctcatg tcgacattct ttgccaaaat gatgagacag cacaataacc agcacgttgc
     7021 ccaggagctg taggaaaaag aagaaggcat gaacatggtt agcagaggct ctagagccgc
     7081 cggtcacacg ccagaagccg aaccccgccc tgccccgtcc cccccgaagg cagccgtccc
     7141 cccgcggaca gccccgaggc tggagaggga gaaggggacg gcggcgcggc gacgcacgaa
     7201 ggccctcccc gcccatttcc ttcctgccgg cgccgcaccg cttcgccccg cgcccgctag
     7261 agggggtgcg gcggcgcctc ccagatttcg gctccgcaca gatttgggac aaaggaagtc
     7321 cctgcgccct ctcgcacgat taccataaaa ggcaatggct gcggctcgcc gcgcctcgac
     7381 agccgccggc gctccggggg ccgccgcgcc cctcccccga gccctccccg gcccgaggcg
     7441 gccccgcccc gcccggcacc cccacctgcc gccacccccc gcccggcacg gcgagccccg
     7501 cgccacgccc cgtacggagc cccgcacccg aagccgggcc gtgctcagca actcggggag
     7561 gggggtgcag ggggggttgc agcccgaccg acgcgcccac accccctgct caccccccca
     7621 cgcacacacc ccgcacgcag cctttgttcc cctcgcagcc ccccccgcac cgcggggcac
     7681 cgcccccggc cgcgctcccc tcgcgcacac tgcggagcgc acaaagcccc gcgccgcgcc
     7741 cgcagcgctc acagccgccg ggcagcgcgg agccgcacgc ggcgctcccc acgcacacac
     7801 acacgcacgc accccccgag ccgctccccc cgcacaaagg gccctcccgg agcccctcaa
     7861 ggctttcacg cagccacaga aaagaaacaa gccgtcatta aaccaagcgc taattacagc
     7921 ccggaggaga agggccgtcc cgcccgctca cctgtgggag taacgcggtc agtcagagcc
     7981 ggggcgggcg gcgcgaggcg gcggcggagc ggggcacggg gcgaaggcag cgtcgcagcg
     8041 actccccgcc cgccgcgcgc ttcgcttttt atagggccgc cgccgccgcc gcctcgccat
     8101 aaaaggaaac tttcggagcg cgccgctctg attggctgcc gccgcacctc tccgcctcgc
     8161 cccgccccgc ccctcgcccc gccccgcccc gcctggcgcg cgcccccccc ccccccccgc
     8221 ccccatcgct gcacaaaata attaaaaaat aaataaatac aaaattgggg gtggggaggg
     8281 gggggagatg gggagagtga agcagaacgt ggggctcacc tcgaccatgg taatagcgat
     8341 gactaatacg tagatgtact gccaagtagg aaagtcccat aaggtcatgt actgggcata
     8401 atgccaggcg ggccatttac cgtcattgac gtcaataggg ggcgtacttg gcatatgata
     8461 cacttgatgt actgccaagt gggcagttta ccgtaaatac tccacccatt gacgtcaatg
     8521 gaaagtccct attggcgtta ctatgggaac atacgtcatt attgacgtca atgggcgggg
     8581 gtcgttgggc ggtcagccag gcgggccatt taccgtaagt tatgtaacgc ggaactccat
     8641 atatgggcta tgaactaatg accccgtaat tgattactat taataactag tcaataatca
     8701 atgtcgagac ccatagagcc caccgcatcc ccagcatgcc tgctattgtc ttcccaatcc
     8761 tcccccttgc tgtcctgccc caccccaccc cccagaatag aatgacacct actcagacaa
     8821 tgcgatgcaa tttcctcatt ttattaggaa aggacagtgg gagtggcacc ttccagggtc
     8881 aaggaaggca cgggggaggg gcaaacaaca gatggctggc aactagaagg cacagtcgag
     8941 gctgatcagc gggtttataa cttcgtataa tgtatgctat acgaagttat ggtaccttac
     9001 tcgtccatgc cgccggtgga gtggcggccc tcggcgcgtt cgtactgttc cacgatggtg
     9061 tagtcctcgt tgtgggaggt gatgtccaac ttgatgttga cgttgtaggc gccgggcagc
     9121 tgcacgggct tcttggcctt gtaggtggtc ttgacctcag cgtcgtagtg gccgccgtcc
     9181 ttcagcttca gcctctgctt gatctcgccc ttcagggcgc cgtcctcggg gtacatccgc
     9241 tcggaggagg cctcccagcc catggtcttc ttctgcatta cggggccgtc ggaggggaag
     9301 ttggtgccgc gcagcttcac cttgtagatg aactcgccgt cctgcaggga ggagtcctgg
     9361 gtcacggtca ccacgccgcc gtcctcgaag ttcatcacgc gctcccactt gaagccctcg
     9421 gggaaggaca gcttcaagta gtcggggatg tcggcggggt gcttcacgta ggccttggag
     9481 ccgtacatga actgagggga caggatgtcc caggcgaagg gcagggggcc acccttggtc
     9541 accttcagct tggcggtctg ggtgccctcg taggggcggc cctcgccctc gccctcgatc
     9601 tcgaactcgt ggccgttcac ggagccctcc atgtgcacct tgaagcgcat gaactccttg
     9661 atgatggcca tgttatcctc ctcgcccttg ctcaccatgg tggcgaccgg tagcgctagc
     9721 gtaggcgccg gtcacagctt ggatctgtaa cggcgcagaa cagaaaacga aacaaagacg
     9781 tagagttgag caagcagggt caggcaaagc gtggagagcc ggctgagtct aggtaggctc
     9841 caagggagcg ccggacaaag gcccggtctc gacctgagct ttaaacttac ctagacggcg
     9901 gacgcagttc aggaggcacc acaggcggga ggcggcagaa cgcgactcaa ccggcgtgga
     9961 tggcggcctc aggtagggcg gcgggcgcgt gaaggagaga tgcgagcccc tcgaagcttc
    10021 agctgtgttc tggcggcaaa cccgttgcga aaaagaacgt tcacggcgac tactgcactt
    10081 atatacggtt ctcccccacc ctcgggaaaa aggcggagcc agtacacgac atcactttcc
    10141 cagtttaccc cgcgccacct tctctaggca ccggttcaat tgccgacccc tccccccaac
    10201 ttctcgggga ctgtgggcga tgtgcgctct gcccactgac gggcaccgga gcgatcgcag
    10261 atcatgcatc catagagccc accgcatccc cagcatgcct gctattgtct tcccaatcct
    10321 cccccttgct gtcctgcccc accccacccc ccagaataga atgacaccta ctcagacaat
    10381 gcgatgcaat ttcctcattt tattaggaaa ggacagtggg agtggcacct tccagggtca
    10441 aggaaggcac gggggagggg caaacaacag atggctggca actagaaggc acaggttttt
    10501 agaagaactc gtcaagaagg cgatagaagg cgatgcgctg cgaatcggga gcggcgatac
    10561 cgtaaagcac gaggaagcgg tcagcccatt cgccgccaag ctcttcagca atatcacggg
    10621 tagccaacgc tatgtcctga tagcggtccg ccacacccag ccggccacag tcgatgaatc
    10681 cagaaaagcg gccattttcc accatgatat tcggcaagca ggcatcgcca tgggtcacga
    10741 cgagatcctc gccgtcgggc atgcgcgcct tgagcctggc gaacagttcg gctggcgcga
    10801 gcccctgatg ctcttcgtcc agatcatcct gatcgacaag accggcttcc atccgagtac
    10861 gtgctcgctc gatgcgatgt ttcgcttggt ggtcgaatgg gcaggtagcc ggatcaagcg
    10921 tatgcagccg ccgcattgca tcagccatga tggatacttt ctcggcagga gcaaggtgag
    10981 atgacaggag atcctgcccc ggcacttcgc ccaatagcag ccagtccctt cccgcttcag
    11041 tgacaacgtc gagcacagct gcgcaaggaa cgcccgtcgt ggccagccac gatagccgcg
    11101 ctgcctcgtc ctgcagttca ttcagggcac cggacaggtc ggtcttgaca aaaagaaccg
    11161 ggcgcccctg cgctgacagc cggaacacgg cggcatcaga gcagccgatt gtctgttgtg
    11221 cccagtcata gccgaatagc ctctccaccc aagcggccgg agaacctgcg tgcaatccat
    11281 cttgttcaat cctagggccg ggattctcct ccacgtcacc gcatgttaga agacttcctc
    11341 tgccctctcc gctgccagat ctctcgaggc cctgtgggag gaagagaaga ggtcagataa
    11401 cttcgtataa tgtatgctat acgaagttat gcggccgctg ggagaagggt aagagagctc
    11461 aatcctcatc ttacatagaa ggaagtcagt agataatatc ttacatttaa aagtcaagaa
    11521 ataacagtct gaccatacta tctagaaata tggaagttaa cagtaaggaa gttaaaacag
    11581 ttgaaagtag tttcgtctag aaagaaagac ttggggaaga acaaagaagg ctaattttca
    11641 ttatgagctt tattgtaaca gaaaagagac cactaactca gctaggtttt cccaattcct
    11701 caacactttg gggcacagtg tttagtcaga gctgtgatca ctctctgagc acatggattg
    11761 tatcttaatt ttcatctcat tgaaaatatg taccatttgt ccaagtggaa actgggctga
    11821 agcatgactg tgaccaccac caaagaaatg gctagactcg ctaaggtctg ctggggtgac
    11881 ccgtgattgt gttgtgcttg atcttgttga gcatggctct tttaagtcac catacctgcc
    11941 cctgccaatg caccaaacac caacttaaag ggactttcag cttttgtaag tcagtctcag
    12001 ccttacttct gaaccatttc tttgacttct ttcatgactc tgatcctgtc ccagttttaa
    12061 gcctcccagc caccagtcat gctgttttgc tctgctattt acattcttct ctacctactt
    12121 tggacttgat ggtatgccta ctatattagt tatttattgc tgtgtaacaa accactctga
    12181 aacatagtgg cttacaacca caaccatttt attttattat gaaactgtgg gtcagaatgt
    12241 atattggccc attggcatga ttctcctgct attgaaaaag gaagagtatg agtattcaac
    12301 atttccgtgt cgcccttatt cccttttttg cggcattttg ccttcctgtt tttgctcacc
    12361 cagaaacgct ggtgaaagta aaagatgctg aagatcagtt gggtgcacga gtgggttaca
    12421 tcgaactgga tctcaacagc ggtaagatcc ttgagagttt tcgccccgaa gaacgttttc
    12481 caatgatgag cacttttaaa gttctgctat gtggcgcggt attatcccgt attgacgccg
    12541 ggcaagagca actcggtcgc cgcatacact attctcagaa tgacttggtt gagtactcac
    12601 cagtcacaga aaagcatctt acggatggca tgacagtaag agaattatgc agtgctgcca
    12661 taaccatgag tgataacact gcggccaact tacttctgac aacgatcgga ggaccgaagg
    12721 agctaaccgc ttttttgcac aacatggggg atcatgtaac tcgccttgat cgttgggaac
    12781 cggagctgaa tgaagccata ccaaacgacg agcgtgacac cacgatgcct gtagcaatgg
    12841 caacaacgtt gcgcaaacta ttaactggcg aactacttac tctagcttcc cggcaacaat
    12901 taatagactg gatggaggcg gataaagttg caggaccact tctgcgctcg gcccttccgg
    12961 ctggctggtt tattgctgat aaatctggag ccggtgagcg tgggtctcgc ggtatcattg
    13021 cagcactggg gccagatggt aagccctccc gtatcgtagt tatctacacg acggggagtc
    13081 aggcaactat ggatgaacga aatagacaga tcgctgagat aggtgcctca ctgattaagc
    13141 attggtaact gtcagaccaa gtttactcat atatacttta gattgattta aaacttcatt
    13201 tttaatttaa aaggatctag gtgaagatcc tttttgataa tctcatgacc aaaatccctt
    13261 aacgtgagtt ttcgttccac tgagcgtcag accccgtaga aaagatcaaa ggatcttctt
    13321 gagatccttt ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag
    13381 cggtggtttg tttgccggat caagagctac caactctttt tccgaaggta actggcttca
    13441 gcagagcgca gataccaaat actgtccttc tagtgtagcc gtagttaggc caccacttca
    13501 agaactctgt agcaccgcct acatacctcg ctctgctaat cc
//
