LOCUS       pCDH-EMTB-TurboI        9287 bp DNA     circular SYN 16-AUG-2022
DEFINITION  .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9287)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Aug 16, 2022 from SnapGene Viewer 6.1.0
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..9287
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        2005..2208
                     /label=CMV promoter
                     /note="color: #84a4c0; direction: RIGHT"
     CDS             2297..2329
                     /label=Myc
                     /note="color: #ff8000; direction: RIGHT"
     CDS             2330..3133
                     /label=EMTB
                     /note="color: #0080ff; direction: RIGHT"
     CDS             3203..4159
                     /label=TurboID
                     /note="color: #ff0000; direction: RIGHT"
     CDS             4166..4207
                     /label=V5
                     /note="color: #ff8000; direction: RIGHT"
     promoter        4257..4468
                     /label=EF1a promoter
                     /note="color: #84a4c0; direction: RIGHT"
     CDS             4776..5375
                     /label=PuroR
                     /note="color: #8000ff; direction: RIGHT"
     CDS             complement(7823..8683)
                     /label=AmpR
                     /note="color: #00ff00; direction: LEFT"
ORIGIN
        1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca
       61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta
      121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga
      181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc
      241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta
      301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact
      361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg
      421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct
      481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt
      541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag
      601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt
      661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt
      721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg
      781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag
      841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag
      901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg
      961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag
     1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag
     1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc
     1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga
     1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc
     1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg
     1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata
     1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa
     1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga
     1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa
     1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat
     1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt
     1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg
     1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggttaacttt
     1801 taaaagaaaa ggggggattg gggggtacag tgcaggggaa agaatagtag acataatagc
     1861 aacagacata caaactaaag aattacaaaa acaaattaca aaaattcaaa attttatcga
     1921 tactagtatt atgcccagta catgacctta tgggactttc ctacttggca gtacatctac
     1981 gtattagtca tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga
     2041 tagcggtttg actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg
     2101 ttttggcacc aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg
     2161 caaatgggcg gtaggcgtgt acggtgggag gtctatataa gcagagctcg tttagtgaac
     2221 cgtcagatcg cctggagacg ccatccacgc tgttttgacc tccatagaag attctagagc
     2281 tagcgaattc gccaccatgg agcagaagct catctcagaa gaagacctcg gcgcgccagt
     2341 gcgaagcgaa acagcacccg acagctacaa agtgcaagat aagaaaaatg cctccagccg
     2401 ccctgcctct gcaatttcag gacaaaataa caaccactca ggaaataaac cagaccctcc
     2461 gcctgtgtta cgtgttgatg accggcagcg gctggcccgg gagcgacgtg aggaacggga
     2521 gaaacagcta gctgcaagag aaatagtgtg gttagaaaga gaagagcgag ccaggcagca
     2581 ctacgagaag cacctggaag agcggaagaa gaggttggag gagcagaggc agaaggagga
     2641 gcggaggagg gctgctgtgg aggagaagcg gaggcagaga cttgaggagg acaaagaacg
     2701 ccacgaagct gttgtacggc gcacaatgga aaggagccag aagccaaaac agaagcataa
     2761 ccgttggtcg tggggaggct ctctccatgg gagccctagc atccacagtg cagatccaga
     2821 caggcggtca gtttccacca tgaatctttc gaaatatgtt gatcccgtca ttagcaagcg
     2881 gctctcctct tcatctgcaa ctttactaaa ttctccagat agagctcgcc gcctgcagct
     2941 cagcccatgg gagagcagcg ttgttaacag actcctgacg cccacacatt cgttcctggc
     3001 cagaagtaaa agcacagctg ccttgtctgg agaagcagca tcttgcagcc ccatcatcat
     3061 gccctacaaa gctgcacact ctagaaattc gatggatcga ccaaaactct ttgtaacacc
     3121 acctgagggc tctagcggcg gccgcggatc aggaagcgga tcaggaagcg gatcaggaag
     3181 cggatcagga agcggatcag gaaaagacaa tactgtgcct ctgaagctga tcgctctcct
     3241 ggctaatggc gagttccata gtggcgaaca gctgggagaa accctgggca tgtccagggc
     3301 cgctatcaac aagcacattc agactctgcg cgactggggc gtggacgtgt tcaccgtgcc
     3361 cggaaagggc tactctctgc ccgagcctat cccgctgctg aacgctaaac agattctggg
     3421 acagctggac ggcgggagcg tggcagtcct gcctgtggtc gactccacca atcagtacct
     3481 gctggatcga atcggcgagc tgaagagtgg ggatgcttgc attgcagaat atcagcaggc
     3541 agggagagga agcagaggga ggaaatggtt ctctcctttt ggagctaacc tgtacctgag
     3601 tatgttttgg cgcctgaagc ggggaccagc agcaatcggc ctgggcccgg tcatcggaat
     3661 tgtcatggca gaagcgctgc gaaagctggg agcagacaag gtgcgagtca aatggcccaa
     3721 tgacctgtat ctgcaggata gaaagctggc aggcatcctg gtggagctgg ccggaataac
     3781 aggcgatgct gcacagatcg tcattggcgc cgggattaac gtggctatga ggcgcgtgga
     3841 ggaaagcgtg gtcaatcagg gctggatcac actgcaggaa gcagggatta acctggacag
     3901 gaatactctg gccgctacgc tgatccgaga gctgcgggca gccctggaac tgttcgagca
     3961 ggaaggcctg gctccatatc tgccacggtg ggagaagctg gataacttca tcaatagacc
     4021 cgtgaagctg atcattgggg acaaagagat tttcgggatt agccggggga ttgataaaca
     4081 gggagccctg ctgctggaac aggacggagt tatcaaaccc tggatgggcg gagaaatcag
     4141 tctgcggtct gccgaaaagg ctagcggcaa gcccatcccc aaccccctgc tgggcctgga
     4201 cagcacctaa ggatccgcgg ccgcaaggat ctgcgatcgc tccggtgccc gtcagtgggc
     4261 agagcgcaca tcgcccacag tccccgagaa gttgggggga ggggtcggca attgaacggg
     4321 tgcctagaga aggtggcgcg gggtaaactg ggaaagtgat gtcgtgtact ggctccgcct
     4381 ttttcccgag ggtgggggag aaccgtatat aagtgcagta gtcgccgtga acgttctttt
     4441 tcgcaacggg tttgccgcca gaacacagct gaagcttcga ggggctcgca tctctccttc
     4501 acgcgcccgc cgccctacct gaggccgcca tccacgccgg ttgagtcgcg ttctgccgcc
     4561 tcccgcctgt ggtgcctcct gaactgcgtc cgccgtctag gtaagtttaa agctcaggtc
     4621 gagaccgggc ctttgtccgg cgctcccttg gagcctacct agactcagcc ggctctccac
     4681 gctttgcctg accctgcttg ctcaactcta cgtctttgtt tcgttttctg ttctgcgccg
     4741 ttacagatcc aagctgtgac cggcgcctac gctagatgac cgagtacaag cccacggtgc
     4801 gcctcgccac ccgcgacgac gtccccaggg ccgtacgcac cctcgccgcc gcgttcgccg
     4861 actaccccgc cacgcgccac accgtcgatc cggaccgcca catcgagcgg gtcaccgagc
     4921 tgcaagaact cttcctcacg cgcgtcgggc tcgacatcgg caaggtgtgg gtcgcggacg
     4981 acggcgccgc ggtggcggtc tggaccacgc cggagagcgt cgaagcgggg gcggtgttcg
     5041 ccgagatcgg cccgcgcatg gccgagttga gcggttcccg gctggccgcg cagcaacaga
     5101 tggaaggcct cctggcgccg caccggccca aggagcccgc gtggttcctg gccaccgtcg
     5161 gcgtctcgcc cgaccaccag ggcaagggtc tgggcagcgc cgtcgtgctc cccggagtgg
     5221 aggcggccga gcgcgccggg gtgcccgcct tcctggagac ctccgcgccc cgcaacctcc
     5281 ccttctacga gcggctcggc ttcaccgtca ccgccgacgt cgaggtgccc gaaggaccgc
     5341 gcacctggtg catgacccgc aagcccggtg cctgagtcga caatcaacct ctggattaca
     5401 aaatttgtga aagattgact ggtattctta actatgttgc tccttttacg ctatgtggat
     5461 acgctgcttt aatgcctttg tatcatgcta ttgcttcccg tatggctttc attttctcct
     5521 ccttgtataa atcctggttg ctgtctcttt atgaggagtt gtggcccgtt gtcaggcaac
     5581 gtggcgtggt gtgcactgtg tttgctgacg caacccccac tggttggggc attgccacca
     5641 cctgtcagct cctttccggg actttcgctt tccccctccc tattgccacg gcggaactca
     5701 tcgccgcctg ccttgcccgc tgctggacag gggctcggct gttgggcact gacaattccg
     5761 tggtgttgtc ggggaaatca tcgtcctttc cttggctgct cgcctgtgtt gccacctgga
     5821 ttctgcgcgg gacgtccttc tgctacgtcc cttcggccct caatccagcg gaccttcctt
     5881 cccgcggcct gctgccggct ctgcggcctc ttccgcgtct tcgccttcgc cctcagacga
     5941 gtcggatctc cctttgggcc gcctccccgc ctggtacctt taagaccaat gacttacaag
     6001 gcagctgtag atcttagcca ctttttaaaa gaaaaggggg gactggaagg gctaattcac
     6061 tcccaacgaa aataagatct gctttttgct tgtactgggt ctctctggtt agaccagatc
     6121 tgagcctggg agctctctgg ctaactaggg aacccactgc ttaagcctca ataaagcttg
     6181 ccttgagtgc ttcaagtagt gtgtgcccgt ctgttgtgtg actctggtaa ctagagatcc
     6241 ctcagaccct tttagtcagt gtggaaaatc tctagcagta gtagttcatg tcatcttatt
     6301 attcagtatt tataacttgc aaagaaatga atatcagaga gtgagaggaa cttgtttatt
     6361 gcagcttata atggttacaa ataaagcaat agcatcacaa atttcacaaa taaagcattt
     6421 ttttcactgc attctagttg tggtttgtcc aaactcatca atgtatctta tcatgtctgg
     6481 ctctagctat cccgccccta actccgccca gttccgccca ttctccgccc catggctgac
     6541 taattttttt tatttatgca gaggccgagg ccgcctcggc ctctgagcta ttccagaagt
     6601 agtgaggagg cttttttgga ggcctagact tttgcagaga cggcccaaat tcgtaatcat
     6661 ggtcatagct gtttcctgtg tgaaattgtt atccgctcac aattccacac aacatacgag
     6721 ccggaagcat aaagtgtaaa gcctggggtg cctaatgagt gagctaactc acattaattg
     6781 cgttgcgctc actgcccgct ttccagtcgg gaaacctgtc gtgccagctg cattaatgaa
     6841 tcggccaacg cgcggggaga ggcggtttgc gtattgggcg ctcttccgct tcctcgctca
     6901 ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg
     6961 taatacggtt atccacagaa tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc
     7021 agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc gtttttccat aggctccgcc
     7081 cccctgacga gcatcacaaa aatcgacgct caagtcagag gtggcgaaac ccgacaggac
     7141 tataaagata ccaggcgttt ccccctggaa gctccctcgt gcgctctcct gttccgaccc
     7201 tgccgcttac cggatacctg tccgcctttc tcccttcggg aagcgtggcg ctttctcata
     7261 gctcacgctg taggtatctc agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc
     7321 acgaaccccc cgttcagccc gaccgctgcg ccttatccgg taactatcgt cttgagtcca
     7381 acccggtaag acacgactta tcgccactgg cagcagccac tggtaacagg attagcagag
     7441 cgaggtatgt aggcggtgct acagagttct tgaagtggtg gcctaactac ggctacacta
     7501 gaaggacagt atttggtatc tgcgctctgc tgaagccagt taccttcgga aaaagagttg
     7561 gtagctcttg atccggcaaa caaaccaccg ctggtagcgg tggttttttt gtttgcaagc
     7621 agcagattac gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt tctacggggt
     7681 ctgacgctca gtggaacgaa aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa
     7741 ggatcttcac ctagatcctt ttaaattaaa aatgaagttt taaatcaatc taaagtatat
     7801 atgagtaaac ttggtctgac agttaccaat gcttaatcag tgaggcacct atctcagcga
     7861 tctgtctatt tcgttcatcc atagttgcct gactccccgt cgtgtagata actacgatac
     7921 gggagggctt accatctggc cccagtgctg caatgatacc gcgagaccca cgctcaccgg
     7981 ctccagattt atcagcaata aaccagccag ccggaagggc cgagcgcaga agtggtcctg
     8041 caactttatc cgcctccatc cagtctatta attgttgccg ggaagctaga gtaagtagtt
     8101 cgccagttaa tagtttgcgc aacgttgttg ccattgctac aggcatcgtg gtgtcacgct
     8161 cgtcgtttgg tatggcttca ttcagctccg gttcccaacg atcaaggcga gttacatgat
     8221 cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc tccgatcgtt gtcagaagta
     8281 agttggccgc agtgttatca ctcatggtta tggcagcact gcataattct cttactgtca
     8341 tgccatccgt aagatgcttt tctgtgactg gtgagtactc aaccaagtca ttctgagaat
     8401 agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaat acgggataat accgcgccac
     8461 atagcagaac tttaaaagtg ctcatcattg gaaaacgttc ttcggggcga aaactctcaa
     8521 ggatcttacc gctgttgaga tccagttcga tgtaacccac tcgtgcaccc aactgatctt
     8581 cagcatcttt tactttcacc agcgtttctg ggtgagcaaa aacaggaagg caaaatgccg
     8641 caaaaaaggg aataagggcg acacggaaat gttgaatact catactcttc ctttttcaat
     8701 attattgaag catttatcag ggttattgtc tcatgagcgg atacatattt gaatgtattt
     8761 agaaaaataa acaaataggg gttccgcgca catttccccg aaaagtgcca cctgacgtct
     8821 aagaaaccat tattatcatg acattaacct ataaaaatag gcgtatcacg aggccctttc
     8881 gtctcgcgcg tttcggtgat gacggtgaaa acctctgaca catgcagctc ccggagacgg
     8941 tcacagcttg tctgtaagcg gatgccggga gcagacaagc ccgtcagggc gcgtcagcgg
     9001 gtgttggcgg gtgtcggggc tggcttaact atgcggcatc agagcagatt gtactgagag
     9061 tgcaccatat gcggtgtgaa ataccgcaca gatgcgtaag gagaaaatac cgcatcaggc
     9121 gccattcgcc attcaggctg cgcaactgtt gggaagggcg atcggtgcgg gcctcttcgc
     9181 tattacgcca gctggcgaaa gggggatgtg ctgcaaggcg attaagttgg gtaacgccag
     9241 ggttttccca gtcacgacgt tgtaaaacga cggccagtgc caagctg
//
