LOCUS       Exported                6482 bp DNA     circular SYN 16-AUG-2022
DEFINITION  .
ACCESSION   pVec1
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6482)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Aug 16, 2022 from SnapGene 6.0.2
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..6482
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..589
                     /label=CMV
                     /note="/vntifkey=29"
                     /note="color: #ffffff"
     misc_feature    679..1647
                     /label=CD86
                     /note="color: #a6acb3"
     misc_feature    1648..1677
                     /label=linker
                     /note="/vntifkey=21"
                     /note="color: #a6acb3"
     misc_feature    1678..2394
                     /label=mEYFP(Q69K)
                     /note="/vntifkey=21"
                     /note="color: #a6acb3"
     misc_feature    1885..1887
                     /label=Q69K
                     /note="/vntifkey=21"
                     /note="color: #a6acb3"
     misc_feature    2269..2307
                     /label=T203Y (EYFP)
                     /note="/vntifkey=21"
                     /note="color: #a6acb3"
     misc_feature    2296..2298
                     /label=A206K
                     /note="/vntifkey=21"
                     /note="color: #a6acb3"
     misc_feature    2331..2352
                     /label=EGFP_C_primer
                     /note="/vntifkey=21"
                     /note="color: #a6acb3"
     misc_feature    2395..2427
                     /label=linker
                     /note="/vntifkey=21"
                     /note="color: #a6acb3"
     CDS$CDS_pept    2428..3147
                     /label=EGFP
                     /note="/vntifkey=4"
                     /note="color: #a6acb3"
     unsure          2428..3147
                     /label=sequence verified
                     /note="/vntifkey=99"
                     /note="color: #a6acb3"
     unsure          3046..3048
                     /label=A206K
                     /note="/vntifkey=99"
                     /note="color: #a6acb3"
     rep_origin      3301..3351
                     /label=SV40 ori
                     /note="/vntifkey=33"
                     /note="color: #ffff00"
     misc_feature    4378..5172
                     /label=kan/neo r
                     /note="/vntifkey=21"
                     /note="color: #a6acb3"
     rep_origin      5757..6400
                     /label=pUC
                     /note="/vntifkey=33"
                     /note="color: #ffff00"
ORIGIN
        1 tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata tggagttccg
       61 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt
      121 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca
      181 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc
      241 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta
      301 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac
      361 catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg
      421 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg
      481 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt
      541 acggtgggag gtctatataa gcagagctgg tttagtgaac cgtcagatcc gctagcgcta
      601 ccggactcag atctcgagct caagcttcga attctgcagt cgacggtacc gcgggcccgg
      661 gatccaccgg tcgccaccat gggactgagt aacattctct ttgtgatggc cttcctgctc
      721 tctggtgctg ctcctctgaa gattcaagct tatttcaatg agactgcaga cctgccatgc
      781 caatttgcaa actctcaaaa ccaaagcctg agtgagctag tagtattttg gcaggaccag
      841 gaaaacttgg ttctgaatga ggtatactta ggcaaagaga aatttgacag tgttcattcc
      901 aagtatatgg gccgcacaag ttttgattcg gacagttgga ccctgagact tcacaatctt
      961 cagatcaagg acaagggctt gtatcaatgt atcatccatc acaaaaagcc cacaggaatg
     1021 attcgcatcc accagatgaa ttctgaactg tcagtgcttg ctaacttcag tcaacctgaa
     1081 atagtaccaa tttctaatat aacagaaaat gtgtacataa atttgacctg ctcatctata
     1141 cacggttacc cagaacctaa gaagatgagt gttttgctaa gaaccaagaa ttcaactatc
     1201 gagtatgatg gtattatgca gaaatctcaa gataatgtca cagaactgta cgacgtttcc
     1261 atcagcttgt ctgtttcatt ccctgatgtt acgagcaata tgaccatctt ctgtattctg
     1321 gaaactgaca agacgcggct tttatcttca cctttctcta tagagcttga ggaccctcag
     1381 cctcccccag accacattcc ttggattaca gctgtacttc caacagttat tatatgtgtg
     1441 atggttttct gtctaattct atggaaatgg aagaagaaga agcggcctcg caactcttat
     1501 aaatgtggaa ccaacacaat ggagagggaa gagagtgaac agaccaagaa aagagaaaaa
     1561 atccatatac ctgaaagatc tgatgaaacc cagcgtgttt ttaaaagttc gaagacatct
     1621 tcatgcgaca aaagtgatac atgttttgga ggcggaccgg tgccccagtg ggagggaatg
     1681 gtgagcaagg gcgaggagct gttcaccggg gtggtgccca tcctggtcga gctggacggc
     1741 gacgtaaacg gccacaagtt cagcgtgtcc ggcgagggcg agggcgatgc cacctacggc
     1801 aagctgaccc tgaagttcat ctgcaccacc ggcaagctgc ccgtgccctg gcccaccctc
     1861 gtgaccacct tcggctacgg cctgaagtgc ttcgcccgct accccgacca catgaagcag
     1921 cacgacttct tcaagtccgc catgcccgaa ggctacgtcc aggagcgcac catcttcttc
     1981 aaggacgacg gcaactacaa gacccgcgcc gaggtgaagt tcgagggcga caccctggtg
     2041 aaccgcatcg agctgaaggg catcgacttc aaggaggacg gcaacatcct ggggcacaag
     2101 ctggagtaca actacaacag ccacaacgtc tatatcatgg ccgacaagca gaagaacggc
     2161 atcaaggtga acttcaagat ccgccacaac atcgaggacg gcagcgtgca gctcgccgac
     2221 cactaccagc agaacacccc catcggcgac ggccccgtgc tgctgcccga caaccactac
     2281 ctgagctacc agtccaagct gagcaaagac cccaacgaga agcgcgatca catggtcctg
     2341 ctggagttcg tgaccgccgc cgggatcact ctcggcatgg acgagctgta caagttcgct
     2401 gcgctgctgg caaccccggt cgctactatg gtgagcaagg gcgaggagct gttcaccggg
     2461 gtggtgccca tcctggtcga gctggacggc gacgtaaacg gccacaagtt cagcgtgtcc
     2521 ggcgagggcg agggcgatgc cacctacggc aagctgaccc tgaagttcat ctgcaccacc
     2581 ggcaagctgc ccgtgccctg gcccaccctc gtgaccaccc tgacctacgg cgtgcagtgc
     2641 ttcagccgct accccgacca catgaagcag cacgacttct tcaagtccgc catgcccgaa
     2701 ggctacgtcc aggagcgcac catcttcttc aaggacgacg gcaactacaa gacccgcgcc
     2761 gaggtgaagt tcgagggcga caccctggtg aaccgcatcg agctgaaggg catcgacttc
     2821 aaggaggacg gcaacatcct ggggcacaag ctggagtaca actacaacag ccacaacgtc
     2881 tatatcatgg ccgacaagca gaagaacggc atcaaggtga acttcaagat ccgccacaac
     2941 atcgaggacg gcagcgtgca gctcgccgac cactaccagc agaacacccc catcggcgac
     3001 ggccccgtgc tgctgcccga caaccactac ctgagcaccc agtccaagct gagcaaagac
     3061 cccaacgaga agcgcgatca catggtcctg ctggagttcg tgaccgccgc cgggatcact
     3121 ctcggcatgg acgagctgta caagtaaagc ggccgcgact ctagatcata atcagccata
     3181 ccacatttgt agaggtttta cttgctttaa aaaacctccc acacctcccc ctgaacctga
     3241 aacataaaat gaatgcaatt gttgttgtta acttgtttat tgcagcttat aatggttaca
     3301 aataaagcaa tagcatcaca aatttcacaa ataaagcatt tttttcactg cattctagtt
     3361 gtggtttgtc caaactcatc aatgtatctt aaggcgtaaa ttgtaagcgt taatattttg
     3421 ttaaaattcg cgttaaattt ttgttaaatc agctcatttt ttaaccaata ggccgaaatc
     3481 ggcaaaatcc cttataaatc aaaagaatag accgagatag ggttgagtgt tgttccagtt
     3541 tggaacaaga gtccactatt aaagaacgtg gactccaacg tcaaagggcg aaaaaccgtc
     3601 tatcagggcg atggcccact acgtgaacca tcaccctaat caagtttttt ggggtcgagg
     3661 tgccgtaaag cactaaatcg gaaccctaaa gggagccccc gatttagagc ttgacgggga
     3721 aagccggcga acgtggcgag aaaggaaggg aagaaagcga aaggagcggg cgctagggcg
     3781 ctggcaagtg tagcggtcac gctgcgcgta accaccacac ccgccgcgct taatgcgccg
     3841 ctacagggcg cgtcaggtgg cacttttcgg ggaaatgtgc gcggaacccc tatttgttta
     3901 tttttctaaa tacattcaaa tatgtatccg ctcatgagac aataaccctg ataaatgctt
     3961 caataatatt gaaaaaggaa gagtcctgag gcggaaagaa ccagctgtgg aatgtgtgtc
     4021 agttagggtg tggaaagtcc ccaggctccc cagcaggcag aagtatgcaa agcatgcatc
     4081 tcaattagtc agcaaccagg tgtggaaagt ccccaggctc cccagcaggc agaagtatgc
     4141 aaagcatgca tctcaattag tcagcaacca tagtcccgcc cctaactccg cccatcccgc
     4201 ccctaactcc gcccagttcc gcccattctc cgccccatgg ctgactaatt ttttttattt
     4261 atgcagaggc cgaggccgcc tcggcctctg agctattcca gaagtagtga ggaggctttt
     4321 ttggaggcct aggcttttgc aaagatcgat caagagacag gatgaggatc gtttcgcatg
     4381 attgaacaag atggattgca cgcaggttct ccggccgctt gggtggagag gctattcggc
     4441 tatgactggg cacaacagac aatcggctgc tctgatgccg ccgtgttccg gctgtcagcg
     4501 caggggcgcc cggttctttt tgtcaagacc gacctgtccg gtgccctgaa tgaactgcaa
     4561 gacgaggcag cgcggctatc gtggctggcc acgacgggcg ttccttgcgc agctgtgctc
     4621 gacgttgtca ctgaagcggg aagggactgg ctgctattgg gcgaagtgcc ggggcaggat
     4681 ctcctgtcat ctcaccttgc tcctgccgag aaagtatcca tcatggctga tgcaatgcgg
     4741 cggctgcata cgcttgatcc ggctacctgc ccattcgacc accaagcgaa acatcgcatc
     4801 gagcgagcac gtactcggat ggaagccggt cttgtcgatc aggatgatct ggacgaagag
     4861 catcaggggc tcgcgccagc cgaactgttc gccaggctca aggcgagcat gcccgacggc
     4921 gaggatctcg tcgtgaccca tggcgatgcc tgcttgccga atatcatggt ggaaaatggc
     4981 cgcttttctg gattcatcga ctgtggccgg ctgggtgtgg cggaccgcta tcaggacata
     5041 gcgttggcta cccgtgatat tgctgaagag cttggcggcg aatgggctga ccgcttcctc
     5101 gtgctttacg gtatcgccgc tcccgattcg cagcgcatcg ccttctatcg ccttcttgac
     5161 gagttcttct gagcgggact ctggggttcg aaatgaccga ccaagcgacg cccaacctgc
     5221 catcacgaga tttcgattcc accgccgcct tctatgaaag gttgggcttc ggaatcgttt
     5281 tccgggacgc cggctggatg atcctccagc gcggggatct catgctggag ttcttcgccc
     5341 accctagggg gaggctaact gaaacacgga aggagacaat accggaagga acccgcgcta
     5401 tgacggcaat aaaaagacag aataaaacgc acggtgttgg gtcgtttgtt cataaacgcg
     5461 gggttcggtc ccagggctgg cactctgtcg ataccccacc gagaccccat tggggccaat
     5521 acgcccgcgt ttcttccttt tccccacccc accccccaag ttcgggtgaa ggcccagggc
     5581 tcgcagccaa cgtcggggcg gcaggccctg ccatagcctc aggttactca tatatacttt
     5641 agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata
     5701 atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag
     5761 aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa
     5821 caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt
     5881 ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc
     5941 cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa
     6001 tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa
     6061 gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc
     6121 ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa
     6181 gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa
     6241 caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg
     6301 ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc
     6361 tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg
     6421 ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgccatgc
     6481 at
//
