LOCUS       Exported                7871 bp DNA     circular SYN 16-AUG-2022
DEFINITION  (Invitrogen Vector).
ACCESSION   pmEGFP(A206K)-hDRD2s
VERSION     .
KEYWORDS    pmEGFP(A206K)-hDRD2s
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7871)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Aug 16, 2022 from SnapGene 6.0.2
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..7871
                     /label=Source_1
                     /note="/invitrogen=146"
                     /note="/vntifkey=98"
                     /note="color: #ffffff"
                     /organism="synthetic DNA construct"
     promoter        232..819
                     /label=CMV promoter
                     /note="/invitrogen=2180000"
                     /note="/vntifkey=29"
                     /note="color: #ffffff"
     variation       440
                     /label=addgene mutation
                     /label=nonstandard type: mutation
                     /note="in the addgene sequence this C is a G"
                     /note="/vntifkey=62"
                     /note="color: #a6acb3"
     primer_bind     769..789
                     /label=CMV forward primer
                     /note="color: black; sequence: cgcaaatgggcggtaggcgtg"
     unsure          811..3265
                     /label=sequence confirmed
                     /note="/vntifkey=99"
                     /note="color: #a6acb3"
     primer_bind     863..882
                     /label=T7 primer
                     /note="color: black; sequence: taatacgactcactataggg"
     promoter        863..879
                     /label=T7 promoter
                     /note="/invitrogen=2370000"
                     /note="/vntifkey=30"
                     /note="color: #ffffff"
     misc_feature    925..1014
                     /label=a7-AChR-signal peptide
                     /note="/vntifkey=21"
                     /note="color: #a6acb3"
     loci            1015..1026
                     /label=linker
                     /note="/vntifkey=18"
                     /note="color: #a6acb3"
     misc_feature    1027..1740
                     /label=EGFP
                     /note="/vntifkey=21"
                     /note="color: #a6acb3"
     misc_feature    1645..1647
                     /label=A206K
                     /note="/vntifkey=21"
                     /note="color: #a6acb3"
     misc_feature    1741..2979
                     /label=hDRD2short
                     /note="/vntifkey=21"
                     /note="color: #a6acb3"
     misc_feature    3186..3280
                     /label=MCS
                     /note="/invitrogen=1630000"
                     /note="/vntifkey=21"
                     /note="color: #a6acb3"
     primer_bind     complement(3292..3309)
                     /label=BGH reverse primer
                     /note="color: black; sequence: tagaaggcacagtcgagg"
     polyA_site      3298..3522
                     /label=BGH pA
                     /note="/invitrogen=1870000"
                     /note="/vntifkey=26"
                     /note="color: #a6acb3"
     rep_origin      3568..3996
                     /label=f1 origin
                     /note="/invitrogen=2500000"
                     /note="/vntifkey=33"
                     /note="color: #ffff00"
     promoter        4001..4371
                     /label=SV40 early promoter
                     /note="/invitrogen=2290000"
                     /note="/vntifkey=29"
                     /note="color: #ffffff"
     CDS$CDS_pept    4389..5414
                     /label=Hygro(R)
                     /note="/invitrogen=1180000"
                     /note="/vntifkey=4"
                     /note="color: #a6acb3"
     polyA_signal    5544..5674
                     /label=SV40 pA
                     /note="/invitrogen=1840000"
                     /note="/vntifkey=25"
                     /note="color: #a6acb3"
     rep_origin      complement(6057..6730)
                     /direction=LEFT
                     /label=pUC origin
                     /note="/invitrogen=2540000"
                     /note="/vntifkey=33"
                     /note="color: #ffff00"
     CDS$CDS_pept    complement(6875..7735)
                     /direction=LEFT
                     /label=Amp(R)
                     /note="/invitrogen=1020000"
                     /note="/vntifkey=4"
                     /note="color: #a6acb3"
     promoter        complement(7736..7834)
                     /label=bla promoter
                     /note="/invitrogen=2330000"
                     /note="/vntifkey=30"
                     /note="color: #ffffff; direction: LEFT"
ORIGIN
        1 gacggatcgg gagatctccc gatcccctat ggtcgactct cagtacaatc tgctctgatg
       61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg
      121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc
      181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt
      241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata
      301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc
      361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc
      421 attgacgtca atgggtggac tatttacggt aaactgccca cttggcagta catcaagtgt
      481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt
      541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca
      601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg
      661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc
      721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg
      781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca
      841 ctgcttactg gcttatcgaa attaatacga ctcactatag ggagacccaa gctggctaga
      901 ctcgagggcc gcccagcttc caccatgggc ctccgggcgc tgatgctgtg gttgctggcg
      961 gcgacggggc tcgtgcgcga gtccctgcaa ggagagttcc aaaggaagct gtacccggtc
     1021 gccaccatgg tgagcaaggg cgaggagctg ttcaccgggg tggtgcccat cctggtcgag
     1081 ctggacggcg acgtaaacgg ccacaagttc agcgtgtccg gcgagggcga gggcgatgcc
     1141 acctacggca agctgaccct gaagttcatc tgcaccaccg gcaagctgcc cgtgccctgg
     1201 cccaccctcg tgaccaccct gacctacggc gtgcagtgct tcagccgcta ccccgaccac
     1261 atgaagcagc acgacttctt caagtccgcc atgcccgaag gctacgtcca ggagcgcacc
     1321 atcttcttca aggacgacgg caactacaag acccgcgccg aggtgaagtt cgagggcgac
     1381 accctggtga accgcatcga gctgaagggc atcgacttca aggaggacgg caacatcctg
     1441 gggcacaagc tggagtacaa ctacaacagc cacaacgtct atatcatggc cgacaagcag
     1501 aagaacggca tcaaggtgaa cttcaagatc cgccacaaca tcgaggacgg cagcgtgcag
     1561 ctcgccgacc actaccagca gaacaccccc atcggcgacg gccccgtgct gctgcccgac
     1621 aaccactacc tgagcaccca gtccaagctg agcaaagacc ccaacgagaa gcgcgatcac
     1681 atggtcctgc tggagttcgt gaccgccgcc gggatcactc tcggcatgga cgagctgtac
     1741 gatccactga atctgtcctg gtatgatgat gatctggaga ggcagaactg gagccggccc
     1801 ttcaacgggt cagacgggaa ggcggacaga ccccactaca actactatgc cacactgctc
     1861 accctgctca tcgctgtcat cgtcttcggc aacgtgctgg tgtgcatggc tgtgtctaga
     1921 gagaaggcgc tgcagaccac caccaactac ctgatcgtca gcctcgcagt ggccgacctc
     1981 ctcgtcgcca cactggtcat gccctgggtt gtctacctgg aggtggtagg tgagtggaaa
     2041 ttcagcagga ttcactgtga catcttcgtc actctggacg tcatgatgtg cacggcgagc
     2101 atcctgaact tgtgtgccat cagcatcgac aggtacacag ctgtggccat gcccatgctg
     2161 tacaatacgc gctacagctc caagcgccgg gtcaccgtca tgatctccat cgtctgggtc
     2221 ctgtccttca ccatctcctg cccactcctc ttcggactca ataacgcaga ccagaacgag
     2281 tgcatcattg ccaacccggc cttcgtggtc tactcctcca tcgtctcctt ctacgtgccc
     2341 ttcattgtca ccctgctggt ctacatcaag atctacattg tcctccgcag acgccgcaag
     2401 cgagtcaaca ccaaacgcag cagccgagct ttcagggccc acctgagggc tccactaaag
     2461 gaggctgccc ggcgagccca ggagctggag atggagatgc tctccagcac cagcccaccc
     2521 gagaggaccc ggtacagccc catcccaccc agccaccacc agctgactct ccccgacccg
     2581 tcccaccacg gtctccacag cactcctgac agccccgcca aaccagagaa gaatgggcat
     2641 gccaaagacc accccaagat tgccaagatc tttgagatcc agaccatgcc caatggcaaa
     2701 acccggacct ccctcaagac catgagccgt aggaagctct cccagcagaa ggagaagaaa
     2761 gccactcaga tgctcgccat tgttctcggc gtgttcatca tctgctggct gcccttcttc
     2821 atcacacaca tcctgaacat acactgtgac tgcaacatcc cgcctgtcct gtacagcgcc
     2881 ttcacgtggc tgggctatgt caacagcgcc gtgaacccca tcatctacac caccttcaac
     2941 attgagttcc gcaaggcctt cctgaagatc ctccactgct gactctgctg cctgcccgca
     3001 cagcagcctg cttcccacct ccctgcccag gccagccagc ctcacccttg cgaaccgtga
     3061 gcaggaaggc ctgggtggat cggcctcctc ttcaccccgg caggccctgc agtgttcgct
     3121 tggctccatg ctcctcactg cccgcacacc ctcactctgc cagggcagtg ctagtgagct
     3181 gggcatggta ccgagctcgg atccactagt ccagtgtggt ggaattctgc agatatccag
     3241 cacagtggcg gccgctcgag tctagagggc ccgtttaaac ccgctgatca gcctcgactg
     3301 tgccttctag ttgccagcca tctgttgttt gcccctcccc cgtgccttcc ttgaccctgg
     3361 aaggtgccac tcccactgtc ctttcctaat aaaatgagga aattgcatcg cattgtctga
     3421 gtaggtgtca ttctattctg gggggtgggg tggggcagga cagcaagggg gaggattggg
     3481 aagacaatag caggcatgct ggggatgcgg tgggctctat ggcttctgag gcggaaagaa
     3541 ccagctgggg ctctaggggg tatccccacg cgccctgtag cggcgcatta agcgcggcgg
     3601 gtgtggtggt tacgcgcagc gtgaccgcta cacttgccag cgccctagcg cccgctcctt
     3661 tcgctttctt cccttccttt ctcgccacgt tcgccggctt tccccgtcaa gctctaaatc
     3721 ggggcatccc tttagggttc cgatttagtg ctttacggca cctcgacccc aaaaaacttg
     3781 attagggtga tggttcacgt agtgggccat cgccctgata gacggttttt cgccctttga
     3841 cgttggagtc cacgttcttt aatagtggac tcttgttcca aactggaaca acactcaacc
     3901 ctatctcggt ctattctttt gatttataag ggattttggg gatttcggcc tattggttaa
     3961 aaaatgagct gatttaacaa aaatttaacg cgaattaatt ctgtggaatg tgtgtcagtt
     4021 agggtgtgga aagtccccag gctccccagg caggcagaag tatgcaaagc atgcatctca
     4081 attagtcagc aaccaggtgt ggaaagtccc caggctcccc agcaggcaga agtatgcaaa
     4141 gcatgcatct caattagtca gcaaccatag tcccgcccct aactccgccc atcccgcccc
     4201 taactccgcc cagttccgcc cattctccgc cccatggctg actaattttt tttatttatg
     4261 cagaggccga ggccgcctct gcctctgagc tattccagaa gtagtgagga ggcttttttg
     4321 gaggcctagg cttttgcaaa aagctcccgg gagcttgtat atccattttc ggatctgatc
     4381 agcacgtgat gaaaaagcct gaactcaccg cgacgtctgt cgagaagttt ctgatcgaaa
     4441 agttcgacag cgtctccgac ctgatgcagc tctcggaggg cgaagaatct cgtgctttca
     4501 gcttcgatgt aggagggcgt ggatatgtcc tgcgggtaaa tagctgcgcc gatggtttct
     4561 acaaagatcg ttatgtttat cggcactttg catcggccgc gctcccgatt ccggaagtgc
     4621 ttgacattgg ggaattcagc gagagcctga cctattgcat ctcccgccgt gcacagggtg
     4681 tcacgttgca agacctgcct gaaaccgaac tgcccgctgt tctgcagccg gtcgcggagg
     4741 ccatggatgc gatcgctgcg gccgatctta gccagacgag cgggttcggc ccattcggac
     4801 cgcaaggaat cggtcaatac actacatggc gtgatttcat atgcgcgatt gctgatcccc
     4861 atgtgtatca ctggcaaact gtgatggacg acaccgtcag tgcgtccgtc gcgcaggctc
     4921 tcgatgagct gatgctttgg gccgaggact gccccgaagt ccggcacctc gtgcacgcgg
     4981 atttcggctc caacaatgtc ctgacggaca atggccgcat aacagcggtc attgactgga
     5041 gcgaggcgat gttcggggat tcccaatacg aggtcgccaa catcttcttc tggaggccgt
     5101 ggttggcttg tatggagcag cagacgcgct acttcgagcg gaggcatccg gagcttgcag
     5161 gatcgccgcg gctccgggcg tatatgctcc gcattggtct tgaccaactc tatcagagct
     5221 tggttgacgg caatttcgat gatgcagctt gggcgcaggg tcgatgcgac gcaatcgtcc
     5281 gatccggagc cgggactgtc gggcgtacac aaatcgcccg cagaagcgcg gccgtctgga
     5341 ccgatggctg tgtagaagta ctcgccgata gtggaaaccg acgccccagc actcgtccga
     5401 gggcaaagga atagcacgtg ctacgagatt tcgattccac cgccgccttc tatgaaaggt
     5461 tgggcttcgg aatcgttttc cgggacgccg gctggatgat cctccagcgc ggggatctca
     5521 tgctggagtt cttcgcccac cccaacttgt ttattgcagc ttataatggt tacaaataaa
     5581 gcaatagcat cacaaatttc acaaataaag catttttttc actgcattct agttgtggtt
     5641 tgtccaaact catcaatgta tcttatcatg tctgtatacc gtcgacctct agctagagct
     5701 tggcgtaatc atggtcatag ctgtttcctg tgtgaaattg ttatccgctc acaattccac
     5761 acaacatacg agccggaagc ataaagtgta aagcctgggg tgcctaatga gtgagctaac
     5821 tcacattaat tgcgttgcgc tcactgcccg ctttccagtc gggaaacctg tcgtgccagc
     5881 tgcattaatg aatcggccaa cgcgcgggga gaggcggttt gcgtattggg cgctcttccg
     5941 cttcctcgct cactgactcg ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc
     6001 actcaaaggc ggtaatacgg ttatccacag aatcagggga taacgcagga aagaacatgt
     6061 gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc
     6121 ataggctccg cccccctgac gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa
     6181 acccgacagg actataaaga taccaggcgt ttccccctgg aagctccctc gtgcgctctc
     6241 ctgttccgac cctgccgctt accggatacc tgtccgcctt tctcccttcg ggaagcgtgg
     6301 cgctttctca atgctcacgc tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc
     6361 tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc ggtaactatc
     6421 gtcttgagtc caacccggta agacacgact tatcgccact ggcagcagcc actggtaaca
     6481 ggattagcag agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg tggcctaact
     6541 acggctacac tagaaggaca gtatttggta tctgcgctct gctgaagcca gttaccttcg
     6601 gaaaaagagt tggtagctct tgatccggca aacaaaccac cgctggtagc ggtggttttt
     6661 ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat cctttgatct
     6721 tttctacggg gtctgacgct cagtggaacg aaaactcacg ttaagggatt ttggtcatga
     6781 gattatcaaa aaggatcttc acctagatcc ttttaaatta aaaatgaagt tttaaatcaa
     6841 tctaaagtat atatgagtaa acttggtctg acagttacca atgcttaatc agtgaggcac
     6901 ctatctcagc gatctgtcta tttcgttcat ccatagttgc ctgactcccc gtcgtgtaga
     6961 taactacgat acgggagggc ttaccatctg gccccagtgc tgcaatgata ccgcgagacc
     7021 cacgctcacc ggctccagat ttatcagcaa taaaccagcc agccggaagg gccgagcgca
     7081 gaagtggtcc tgcaacttta tccgcctcca tccagtctat taattgttgc cgggaagcta
     7141 gagtaagtag ttcgccagtt aatagtttgc gcaacgttgt tgccattgct acaggcatcg
     7201 tggtgtcacg ctcgtcgttt ggtatggctt cattcagctc cggttcccaa cgatcaaggc
     7261 gagttacatg atcccccatg ttgtgcaaaa aagcggttag ctccttcggt cctccgatcg
     7321 ttgtcagaag taagttggcc gcagtgttat cactcatggt tatggcagca ctgcataatt
     7381 ctcttactgt catgccatcc gtaagatgct tttctgtgac tggtgagtac tcaaccaagt
     7441 cattctgaga atagtgtatg cggcgaccga gttgctcttg cccggcgtca atacgggata
     7501 ataccgcgcc acatagcaga actttaaaag tgctcatcat tggaaaacgt tcttcggggc
     7561 gaaaactctc aaggatctta ccgctgttga gatccagttc gatgtaaccc actcgtgcac
     7621 ccaactgatc ttcagcatct tttactttca ccagcgtttc tgggtgagca aaaacaggaa
     7681 ggcaaaatgc cgcaaaaaag ggaataaggg cgacacggaa atgttgaata ctcatactct
     7741 tcctttttca atattattga agcatttatc agggttattg tctcatgagc ggatacatat
     7801 ttgaatgtat ttagaaaaat aaacaaatag gggttccgcg cacatttccc cgaaaagtgc
     7861 cacctgacgt c
//
