LOCUS       Exported                8027 bp DNA     circular SYN 16-AUG-2022
DEFINITION  (Invitrogen Vector).
ACCESSION   pmEGFP(A206K)-ADRA2A
VERSION     .
KEYWORDS    pmEGFP(A206K)-ADRA2A
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8027)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Aug 16, 2022 from SnapGene 6.0.2
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..8027
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        232..819
                     /label=CMV promoter
                     /note="/invitrogen=2180000"
                     /note="/vntifkey=29"
                     /note="color: #ffffff"
     variation       440
                     /label=addgene mutation
                     /label=nonstandard type: mutation
                     /note="in the addgene sequence this C is a G"
                     /note="/vntifkey=62"
                     /note="color: #a6acb3"
     primer_bind     769..789
                     /label=CMV forward primer
                     /note="color: black; sequence: cgcaaatgggcggtaggcgtg"
     primer_bind     863..882
                     /label=T7 primer
                     /note="color: black; sequence: taatacgactcactataggg"
     promoter        863..879
                     /label=T7 promoter
                     /note="/invitrogen=2370000"
                     /note="/vntifkey=30"
                     /note="color: #ffffff"
     misc_feature    925..1014
                     /label=a7-AChR-signal peptide
                     /note="/vntifkey=21"
                     /note="color: #a6acb3"
     loci            1015..1026
                     /label=linker
                     /note="/vntifkey=18"
                     /note="color: #a6acb3"
     misc_feature    1027..1740
                     /label=EGFP
                     /note="/vntifkey=21"
                     /note="color: #a6acb3"
     misc_feature    1645..1647
                     /label=A206K
                     /note="/vntifkey=21"
                     /note="color: #a6acb3"
     misc_feature    1741..3135
                     /label=ADRA2A
                     /note="/vntifkey=21"
                     /note="color: #a6acb3"
     misc_feature    3342..3436
                     /label=MCS
                     /note="/invitrogen=1630000"
                     /note="/vntifkey=21"
                     /note="color: #a6acb3"
     primer_bind     complement(3448..3465)
                     /label=BGH reverse primer
                     /note="color: black; sequence: tagaaggcacagtcgagg"
     polyA_site      3454..3678
                     /label=BGH pA
                     /note="/invitrogen=1870000"
                     /note="/vntifkey=26"
                     /note="color: #a6acb3"
     rep_origin      3724..4152
                     /label=f1 origin
                     /note="/invitrogen=2500000"
                     /note="/vntifkey=33"
                     /note="color: #ffff00"
     promoter        4157..4527
                     /label=SV40 early promoter
                     /note="/invitrogen=2290000"
                     /note="/vntifkey=29"
                     /note="color: #ffffff"
     CDS$CDS_pept    4545..5570
                     /label=Hygro(R)
                     /note="/invitrogen=1180000"
                     /note="/vntifkey=4"
                     /note="color: #a6acb3"
     polyA_signal    5700..5830
                     /label=SV40 pA
                     /note="/invitrogen=1840000"
                     /note="/vntifkey=25"
                     /note="color: #a6acb3"
     rep_origin      complement(6213..6886)
                     /direction=LEFT
                     /label=pUC origin
                     /note="/invitrogen=2540000"
                     /note="/vntifkey=33"
                     /note="color: #ffff00"
     CDS$CDS_pept    complement(7031..7891)
                     /direction=LEFT
                     /label=Amp(R)
                     /note="/invitrogen=1020000"
                     /note="/vntifkey=4"
                     /note="color: #a6acb3"
     promoter        complement(7892..7990)
                     /label=bla promoter
                     /note="/invitrogen=2330000"
                     /note="/vntifkey=30"
                     /note="color: #ffffff; direction: LEFT"
ORIGIN
        1 gacggatcgg gagatctccc gatcccctat ggtcgactct cagtacaatc tgctctgatg
       61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg
      121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc
      181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt
      241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata
      301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc
      361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc
      421 attgacgtca atgggtggac tatttacggt aaactgccca cttggcagta catcaagtgt
      481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt
      541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca
      601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg
      661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc
      721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg
      781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca
      841 ctgcttactg gcttatcgaa attaatacga ctcactatag ggagacccaa gctggctaga
      901 ctcgagggcc gcccagcttc caccatgggc ctccgggcgc tgatgctgtg gttgctggcg
      961 gcgacggggc tcgtgcgcga gtccctgcaa ggagagttcc aaaggaagct gtacccggtc
     1021 gccaccatgg tgagcaaggg cgaggagctg ttcaccgggg tggtgcccat cctggtcgag
     1081 ctggacggcg acgtaaacgg ccacaagttc agcgtgtccg gcgagggcga gggcgatgcc
     1141 acctacggca agctgaccct gaagttcatc tgcaccaccg gcaagctgcc cgtgccctgg
     1201 cccaccctcg tgaccaccct gacctacggc gtgcagtgct tcagccgcta ccccgaccac
     1261 atgaagcagc acgacttctt caagtccgcc atgcccgaag gctacgtcca ggagcgcacc
     1321 atcttcttca aggacgacgg caactacaag acccgcgccg aggtgaagtt cgagggcgac
     1381 accctggtga accgcatcga gctgaagggc atcgacttca aggaggacgg caacatcctg
     1441 gggcacaagc tggagtacaa ctacaacagc cacaacgtct atatcatggc cgacaagcag
     1501 aagaacggca tcaaggtgaa cttcaagatc cgccacaaca tcgaggacgg cagcgtgcag
     1561 ctcgccgacc actaccagca gaacaccccc atcggcgacg gccccgtgct gctgcccgac
     1621 aaccactacc tgagcaccca gtccaagctg agcaaagacc ccaacgagaa gcgcgatcac
     1681 atggtcctgc tggagttcgt gaccgccgcc gggatcactc tcggcatgga cgagctgtac
     1741 atgttccggc aggagcagcc actcgccgag ggttcctttg cccccatggg aagtcttcag
     1801 cctgacgccg gcaacgctag ctggaatggc accgaagcgc ccggtggagg ggctcgggct
     1861 accccatatt ctctccaggt cacactgacc ctggtttgtc tggccggtct cctgatgctg
     1921 ctgacggtct ttggcaatgt tttggtgata attgcggtgt tcacctccag agcactcaag
     1981 gctccccaga acctgttctt ggtcagcctg gcgtctgccg atattctggt cgccaccctc
     2041 gtaattccgt tttctcttgc caatgaggtt atgggttact ggtattttgg taaggcatgg
     2101 tgcgagatat accttgctct cgacgttctc ttctgcacca gcagtattgt gcatctgtgc
     2161 gcaattagtc tggacagata ttggtccatc acgcaggcaa tagaatataa cttgaaaagg
     2221 actcctcggc gcattaaggc tatcatcatc actgtctggg tcattagcgc tgtgataagc
     2281 ttccctccac ttatttccat tgaaaaaaaa ggaggaggcg gagggccgca gcctgcggag
     2341 ccccgctgtg agatcaacga tcagaaatgg tatgtcatct cctcctgcat tgggtccttc
     2401 ttcgccccat gtctgatcat gatcctcgta tatgttagga tttatcagat agctaagcga
     2461 aggacccggg tccccccatc caggagaggt ccagacgcag tggccgctcc ccccggaggc
     2521 acagaaagga gacctaacgg tctgggacca gagcgctccg caggacctgg aggtgcagaa
     2581 gccgagccct tgccgacaca actgaacgga gctcccggcg aaccagcccc agcaggtcct
     2641 cgcgatactg acgcactgga cctcgaagag tcctctagca gcgatcatgc agagcgccct
     2701 ccagggccta ggagacctga aagaggaccc agaggtaaag gcaaggcccg ggcaagtcaa
     2761 gtgaaaccgg gagactctct gcctcggagg ggtccaggtg ctactggtat agggactcct
     2821 gccgccggtc ccggagaaga gcgggtcggc gccgctaagg caagtcgctg gcggggcaga
     2881 caaaaccggg aaaaaaggtt tacattcgtg ctcgcggtag tgattggcgt cttcgtcgtg
     2941 tgctggtttc cattcttttt tacctatacc ctgacagccg tcggctgttc tgttccacgg
     3001 acactgttca agttcttttt ctggtttggc tactgtaact caagcctgaa cccggtaatc
     3061 tatacaatat ttaaccacga ttttcggagg gcttttaaaa agatcctgtg cagaggcgat
     3121 aggaaacgaa tcgtttgact ctgctgcctg cccgcacagc agcctgcttc ccacctccct
     3181 gcccaggcca gccagcctca cccttgcgaa ccgtgagcag gaaggcctgg gtggatcggc
     3241 ctcctcttca ccccggcagg ccctgcagtg ttcgcttggc tccatgctcc tcactgcccg
     3301 cacaccctca ctctgccagg gcagtgctag tgagctgggc atggtaccga gctcggatcc
     3361 actagtccag tgtggtggaa ttctgcagat atccagcaca gtggcggccg ctcgagtcta
     3421 gagggcccgt ttaaacccgc tgatcagcct cgactgtgcc ttctagttgc cagccatctg
     3481 ttgtttgccc ctcccccgtg ccttccttga ccctggaagg tgccactccc actgtccttt
     3541 cctaataaaa tgaggaaatt gcatcgcatt gtctgagtag gtgtcattct attctggggg
     3601 gtggggtggg gcaggacagc aagggggagg attgggaaga caatagcagg catgctgggg
     3661 atgcggtggg ctctatggct tctgaggcgg aaagaaccag ctggggctct agggggtatc
     3721 cccacgcgcc ctgtagcggc gcattaagcg cggcgggtgt ggtggttacg cgcagcgtga
     3781 ccgctacact tgccagcgcc ctagcgcccg ctcctttcgc tttcttccct tcctttctcg
     3841 ccacgttcgc cggctttccc cgtcaagctc taaatcgggg catcccttta gggttccgat
     3901 ttagtgcttt acggcacctc gaccccaaaa aacttgatta gggtgatggt tcacgtagtg
     3961 ggccatcgcc ctgatagacg gtttttcgcc ctttgacgtt ggagtccacg ttctttaata
     4021 gtggactctt gttccaaact ggaacaacac tcaaccctat ctcggtctat tcttttgatt
     4081 tataagggat tttggggatt tcggcctatt ggttaaaaaa tgagctgatt taacaaaaat
     4141 ttaacgcgaa ttaattctgt ggaatgtgtg tcagttaggg tgtggaaagt ccccaggctc
     4201 cccaggcagg cagaagtatg caaagcatgc atctcaatta gtcagcaacc aggtgtggaa
     4261 agtccccagg ctccccagca ggcagaagta tgcaaagcat gcatctcaat tagtcagcaa
     4321 ccatagtccc gcccctaact ccgcccatcc cgcccctaac tccgcccagt tccgcccatt
     4381 ctccgcccca tggctgacta atttttttta tttatgcaga ggccgaggcc gcctctgcct
     4441 ctgagctatt ccagaagtag tgaggaggct tttttggagg cctaggcttt tgcaaaaagc
     4501 tcccgggagc ttgtatatcc attttcggat ctgatcagca cgtgatgaaa aagcctgaac
     4561 tcaccgcgac gtctgtcgag aagtttctga tcgaaaagtt cgacagcgtc tccgacctga
     4621 tgcagctctc ggagggcgaa gaatctcgtg ctttcagctt cgatgtagga gggcgtggat
     4681 atgtcctgcg ggtaaatagc tgcgccgatg gtttctacaa agatcgttat gtttatcggc
     4741 actttgcatc ggccgcgctc ccgattccgg aagtgcttga cattggggaa ttcagcgaga
     4801 gcctgaccta ttgcatctcc cgccgtgcac agggtgtcac gttgcaagac ctgcctgaaa
     4861 ccgaactgcc cgctgttctg cagccggtcg cggaggccat ggatgcgatc gctgcggccg
     4921 atcttagcca gacgagcggg ttcggcccat tcggaccgca aggaatcggt caatacacta
     4981 catggcgtga tttcatatgc gcgattgctg atccccatgt gtatcactgg caaactgtga
     5041 tggacgacac cgtcagtgcg tccgtcgcgc aggctctcga tgagctgatg ctttgggccg
     5101 aggactgccc cgaagtccgg cacctcgtgc acgcggattt cggctccaac aatgtcctga
     5161 cggacaatgg ccgcataaca gcggtcattg actggagcga ggcgatgttc ggggattccc
     5221 aatacgaggt cgccaacatc ttcttctgga ggccgtggtt ggcttgtatg gagcagcaga
     5281 cgcgctactt cgagcggagg catccggagc ttgcaggatc gccgcggctc cgggcgtata
     5341 tgctccgcat tggtcttgac caactctatc agagcttggt tgacggcaat ttcgatgatg
     5401 cagcttgggc gcagggtcga tgcgacgcaa tcgtccgatc cggagccggg actgtcgggc
     5461 gtacacaaat cgcccgcaga agcgcggccg tctggaccga tggctgtgta gaagtactcg
     5521 ccgatagtgg aaaccgacgc cccagcactc gtccgagggc aaaggaatag cacgtgctac
     5581 gagatttcga ttccaccgcc gccttctatg aaaggttggg cttcggaatc gttttccggg
     5641 acgccggctg gatgatcctc cagcgcgggg atctcatgct ggagttcttc gcccacccca
     5701 acttgtttat tgcagcttat aatggttaca aataaagcaa tagcatcaca aatttcacaa
     5761 ataaagcatt tttttcactg cattctagtt gtggtttgtc caaactcatc aatgtatctt
     5821 atcatgtctg tataccgtcg acctctagct agagcttggc gtaatcatgg tcatagctgt
     5881 ttcctgtgtg aaattgttat ccgctcacaa ttccacacaa catacgagcc ggaagcataa
     5941 agtgtaaagc ctggggtgcc taatgagtga gctaactcac attaattgcg ttgcgctcac
     6001 tgcccgcttt ccagtcggga aacctgtcgt gccagctgca ttaatgaatc ggccaacgcg
     6061 cggggagagg cggtttgcgt attgggcgct cttccgcttc ctcgctcact gactcgctgc
     6121 gctcggtcgt tcggctgcgg cgagcggtat cagctcactc aaaggcggta atacggttat
     6181 ccacagaatc aggggataac gcaggaaaga acatgtgagc aaaaggccag caaaaggcca
     6241 ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag gctccgcccc cctgacgagc
     6301 atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc gacaggacta taaagatacc
     6361 aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg
     6421 gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct ttctcaatgc tcacgctgta
     6481 ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg
     6541 ttcagcccga ccgctgcgcc ttatccggta actatcgtct tgagtccaac ccggtaagac
     6601 acgacttatc gccactggca gcagccactg gtaacaggat tagcagagcg aggtatgtag
     6661 gcggtgctac agagttcttg aagtggtggc ctaactacgg ctacactaga aggacagtat
     6721 ttggtatctg cgctctgctg aagccagtta ccttcggaaa aagagttggt agctcttgat
     6781 ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag cagattacgc
     6841 gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc tacggggtct gacgctcagt
     6901 ggaacgaaaa ctcacgttaa gggattttgg tcatgagatt atcaaaaagg atcttcacct
     6961 agatcctttt aaattaaaaa tgaagtttta aatcaatcta aagtatatat gagtaaactt
     7021 ggtctgacag ttaccaatgc ttaatcagtg aggcacctat ctcagcgatc tgtctatttc
     7081 gttcatccat agttgcctga ctccccgtcg tgtagataac tacgatacgg gagggcttac
     7141 catctggccc cagtgctgca atgataccgc gagacccacg ctcaccggct ccagatttat
     7201 cagcaataaa ccagccagcc ggaagggccg agcgcagaag tggtcctgca actttatccg
     7261 cctccatcca gtctattaat tgttgccggg aagctagagt aagtagttcg ccagttaata
     7321 gtttgcgcaa cgttgttgcc attgctacag gcatcgtggt gtcacgctcg tcgtttggta
     7381 tggcttcatt cagctccggt tcccaacgat caaggcgagt tacatgatcc cccatgttgt
     7441 gcaaaaaagc ggttagctcc ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag
     7501 tgttatcact catggttatg gcagcactgc ataattctct tactgtcatg ccatccgtaa
     7561 gatgcttttc tgtgactggt gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc
     7621 gaccgagttg ctcttgcccg gcgtcaatac gggataatac cgcgccacat agcagaactt
     7681 taaaagtgct catcattgga aaacgttctt cggggcgaaa actctcaagg atcttaccgc
     7741 tgttgagatc cagttcgatg taacccactc gtgcacccaa ctgatcttca gcatctttta
     7801 ctttcaccag cgtttctggg tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa
     7861 taagggcgac acggaaatgt tgaatactca tactcttcct ttttcaatat tattgaagca
     7921 tttatcaggg ttattgtctc atgagcggat acatatttga atgtatttag aaaaataaac
     7981 aaataggggt tccgcgcaca tttccccgaa aagtgccacc tgacgtc
//
