LOCUS       Exported                9183 bp DNA     circular SYN 16-AUG-2022
DEFINITION  .
ACCESSION   pmCherry2-GNB1-T2A-mCherry2-GNG2-IRES-GNAI1-mYFP
VERSION     .
KEYWORDS    pmCherry2-GNB1-T2A-mCherry2-GNG2-IRES-GNAI1-mYFP
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9183)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Aug 16, 2022 from SnapGene 6.0.2
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..9183
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..589
                     /label=CMV
                     /note="/vntifkey=29"
                     /note="color: #ffffff"
     misc_feature    613..1320
                     /label=mCherry2
                     /note="/vntifkey=21"
                     /note="color: #a6acb3"
     misc_feature    complement(724..745)
                     /label=EGFP_N_primer
                     /note="/vntifkey=21"
                     /note="color: #a6acb3; direction: LEFT"
     primer_bind     complement(764..782)
                     /label=mCherry-R
                     /note="/vntifkey=28"
                     /note="color: #a020f0; direction: LEFT"
     primer_bind     complement(764..782)
                     /label=mCherry-R
                     /note="/vntifkey=28"
                     /note="color: #a020f0; direction: LEFT"
     intron          1321..1374
                     /label=linker
                     /note="/vntifkey=15"
                     /note="color: #------"
     misc_feature    1375..2394
                     /label=GNB1
                     /note="/vntifkey=21"
                     /note="color: #a6acb3"
     promoter        complement(2157..2174)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
                     /note="/vntifkey=30"
                     /note="color: #ffffff; direction: LEFT"
     primer_bind     complement(2157..2174)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
                     /note="/vntifkey=28"
                     /note="color: #a020f0; direction: LEFT"
     primer_bind     complement(2353..2370)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer.
                     Also called pCDH-rev"
                     /note="/vntifkey=28"
                     /note="color: #a020f0; direction: LEFT"
     CDS$CDS_pept    2395..2448
                     /direction=RIGHT
                     /codon_start=1
                     /product="""2A peptide from Thosea asigna virus capsid"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /note="/vntifkey=4"
                     /note="color: #a6acb3"
                     /translation="EGRGSLLTCGDVEENPGP"
     misc_feature    2449..2457
                     /label=linker
                     /note="/vntifkey=21"
                     /note="color: #a6acb3"
     misc_feature    2458..3165
                     /label=mCherry2
                     /note="/vntifkey=21"
                     /note="color: #a6acb3"
     misc_feature    3181..3393
                     /label=GNG2
                     /note="/vntifkey=21"
                     /note="color: #a6acb3"
     misc_feature    3494..4067
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
                     /note="/vntifkey=21"
                     /note="color: #a6acb3"
     misc_feature    3517..4069
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
                     /note="/vntifkey=21"
                     /note="color: #a6acb3"
     primer_bind     3888..3907
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
                     /note="/vntifkey=28"
                     /note="color: #a020f0; direction: RIGHT"
     misc_feature    4069..4431
                     /label=GNAI1
                     /note="/vntifkey=21"
                     /note="color: #a6acb3"
     misc_feature    4432..4440
                     /label=linker
                     /note="/vntifkey=21"
                     /note="color: #a6acb3"
     misc_feature    4441..5133
                     /label=mYFP
                     /note="/vntifkey=21"
                     /note="color: #a6acb3"
     misc_feature    4648..4650
                     /label=Q69K
                     /note="/vntifkey=21"
                     /note="color: #a6acb3"
     misc_feature    5050..5052
                     /label=T203Y (EYFP)
                     /note="/vntifkey=21"
                     /note="color: #a6acb3"
     misc_feature    5059..5061
                     /label=A206K
                     /note="/vntifkey=21"
                     /note="color: #a6acb3"
     misc_feature    5094..5115
                     /label=EGFP_C_primer
                     /note="/vntifkey=21"
                     /note="color: #a6acb3"
     misc_feature    5134..5136
                     /label=linker
                     /note="/vntifkey=21"
                     /note="color: #a6acb3"
     misc_feature    5137..5835
                     /label=GNAI1
                     /note="/vntifkey=21"
                     /note="color: #a6acb3"
     misc_feature    6002..6052
                     /label=sv40 ori
                     /note="/vntifkey=21"
                     /note="color: #a6acb3"
     misc_feature    6099..6554
                     /label=f1
                     /note="/vntifkey=21"
                     /note="color: #a6acb3"
     misc_feature    6728..7030
                     /label=sv40 ori
                     /note="/vntifkey=21"
                     /note="color: #a6acb3"
     CDS$CDS_pept    7079..7873
                     /label=Kan/Neo r
                     /note="/vntifkey=4"
                     /note="color: #a6acb3"
     rep_origin      8458..9101
                     /label=pUC
                     /note="/vntifkey=33"
                     /note="color: #ffff00"
ORIGIN
        1 tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata tggagttccg
       61 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt
      121 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca
      181 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc
      241 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta
      301 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac
      361 catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg
      421 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg
      481 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt
      541 acggtgggag gtctatataa gcagagctgg tttagtgaac cgtcagatcc gctagcgcta
      601 ccggtcgcca ccatggtgag caagggcgag gaggataaca tggccatcat caaggagttc
      661 atgcgcttca aggtgcacat ggagggctcc gtgaacggcc acgagttcga gatcgagggc
      721 gagggcgagg gccgccccta cgagggcacc cagaccgcca agctgaaggt gaccaagggt
      781 ggccccctgc ccttcgcctg ggacatcctg tcccctcagt tcatgtacgg ctccaaggcc
      841 tacgtgaagc accccgccga catccccgac tacttgaagc tgtccttccc cgagggcttc
      901 aattgggagc gcgtgatgaa cttcgaggac ggcggcgtgg tgaccgtgac ccaggactcc
      961 tccctgcagg acggcgagtt catctacaag gtgaagctgc gcggcaccaa cttcccctcc
     1021 gacggccccg taatgcagtg tcgtaccatg ggctgggagg cctccactga gcggatgtac
     1081 cccgaggacg gcgccctgaa gggcgagatc aagcagaggc tgaagctgaa ggacggcggc
     1141 cactacgacg ctgaggtcaa gaccacctac aaggccaaga agcccgtgca gctgcccggc
     1201 gcctacaacg tcgacatcaa gttggacatc ctttcccaca acgaggacta caccatcgtg
     1261 gaacagtacg aacgcgccga gggccgccac tccaccggcg gcatggacga gctgtacaag
     1321 tccggactca gatctcgagc tcaagcttcg aattcactag tgattgccgc caccatgagt
     1381 gagcttgacc agttacggca ggaggccgag caacttaaga accagattcg agacgccagg
     1441 aaagcatgtg cagatgcaac tctctctcag atcacaaaca acatcgaccc agtgggaaga
     1501 atccaaatgc gcacgaggag gacactgcgg gggcacctgg ccaagatcta cgccatgcac
     1561 tggggcacag actccaggct tctcgtcagt gcctcgcagg atggtaaact tatcatctgg
     1621 gacagctaca ccaccaacaa ggtccacgcc atccctctgc gctcctcctg ggtcatgacc
     1681 tgtgcatatg ccccttctgg gaactatgtg gcctgcggtg gcctggataa catttgctcc
     1741 atttacaatc tgaaaactcg tgaggggaac gtgcgcgtga gtcgtgagct ggcaggacac
     1801 acaggttacc tgtcctgctg ccgattcctg gatgacaatc agatcgtcac cagctctgga
     1861 gacaccacgt gtgccctgtg ggacatcgag accggccagc agacgaccac gtttaccgga
     1921 cacactggag atgtcatgag cctttctctt gctcctgaca ccagactgtt cgtctctggt
     1981 gcttgtgatg cttcagccaa actctgggat gtgcgagaag gcatgtgccg gcagaccttc
     2041 actggccacg agtctgacat caatgccatt tgcttctttc caaatggcaa tgcatttgcc
     2101 actggctcag acgacgccac ctgcaggctg tttgaccttc gtgctgacca ggagctcatg
     2161 acttactccc atgacaacat catctgcggg atcacctctg tctccttctc caagagcggg
     2221 cgcctcctcc ttgctgggta cgacgacttc aactgcaacg tctgggatgc actcaaagcc
     2281 gaccgggcag gtgtcttggc tgggcatgac aaccgcgtca gctgcctggg cgtgactgac
     2341 gatggcatgg ctgtggcgac agggtcctgg gatagcttcc tcaagatctg gaacgagggc
     2401 agaggaagtc ttctaacatg cggtgacgtg gaggagaatc ccggccctac cggtatgatg
     2461 gtgagcaagg gcgaggagga taacatggcc atcatcaagg agttcatgcg cttcaaggtg
     2521 cacatggagg gctccgtgaa cggccacgag ttcgagatcg agggcgaggg cgagggccgc
     2581 ccctacgagg gcacccagac cgccaagctg aaggtgacca agggtggccc cctgcccttc
     2641 gcctgggaca tcctgtcccc tcagttcatg tacggctcca aggcctacgt gaagcacccc
     2701 gccgacatcc ccgactactt gaagctgtcc ttccccgagg gcttcaattg ggagcgcgtg
     2761 atgaacttcg aggacggcgg cgtggtgacc gtgacccagg actcctccct gcaggacggc
     2821 gagttcatct acaaggtgaa gctgcgcggc accaacttcc cctccgacgg ccccgtaatg
     2881 cagtgtcgta ccatgggctg ggaggcctcc actgagcgga tgtaccccga ggacggcgcc
     2941 ctgaagggcg agatcaagca gaggctgaag ctgaaggacg gcggccacta cgacgctgag
     3001 gtcaagacca cctacaaggc caagaagccc gtgcagctgc ccggcgccta caacgtcgac
     3061 atcaagttgg acatcctttc ccacaacgag gactacacca tcgtggaaca gtacgaacgc
     3121 gccgagggcc gccactccac cggcggcatg gacgagctgt acaagtccgg actcagatct
     3181 atggccagca acaacaccgc cagcatagca caagccagga agctggtaga gcagcttaag
     3241 atggaagcca atatcgacag gataaaggtg tccaaggcag ctgcagattt gatggcctac
     3301 tgtgaagcac atgccaagga agaccccctc ctgacccctg ttccggcttc agaaaacccg
     3361 tttagggaga agaagttttt ctgtgccatc ctttaaccgc ggcgtacgag gcctgcatgc
     3421 tccggacctg caggttcgaa gtcgacagat ctcaattggg gcccctatag tgtcacctaa
     3481 ataattccgc ccccccctct ccctcccccc cccctaacgt tactggccga agccgcttgg
     3541 aataaggccg gtgtgcgttt gtctatatgt tattttccac catattgccg tcttttggca
     3601 atgtgagggc ccggaaacct ggccctgtct tcttgacgag cattcctagg ggtctttccc
     3661 ctctcgccaa aggaatgcaa ggtctgttga atgtcgtgaa ggaagcagtt cctctggaag
     3721 cttcttgaag acaaacaacg tctgtagcga ccctttgcag gcagcggaac cccccacctg
     3781 gcgacaggtg cctctgcggc caaaagccac gtgtataaga tacacctgca aaggcggcac
     3841 aaccccagtg ccacgttgtg agttggatag ttgtggaaag agtcaaatgg ctctcctcaa
     3901 gcgtattcaa caaggggctg aaggatgccc agaaggtacc ccattgtatg ggatctgatc
     3961 tggggcctcg gtgcacatgc tttacatgtg tttagtcgag gttaaaaaaa cgtctaggcc
     4021 ccccgaacca cggggacgtg gttttccttt gaaaaacacg atgataatat gggctgcaca
     4081 ctgagcgctg aggacaaggc ggccgtggag cgcagcaaga tgatcgaccg caacctccgg
     4141 gaggacggag agaaggcagc gcgcgaggtc aagctgctgc tgctgggtgc tggtgaatcc
     4201 gggaagagca caattgtgaa gcagatgaaa attatccacg aggctggcta ctcagaggaa
     4261 gagtgtaagc agtacaaagc agtggtctac agcaacacca tccagtccat cattgccatc
     4321 attagagcta tggggagatt gaaaatcgac tttggagacg ctgctcgtgc ggatgatgct
     4381 cgccaactct tcgtgcttgc tggggctgca gaggaaggct ttatgaccgc gaccggttct
     4441 atggtgagca agggcgagga gctgttcacc ggggtggtgc ccatcctggt cgagctggac
     4501 ggcgacgtaa acggccacaa gttcagcgtg tccggcgagg gcgagggcga tgccacctac
     4561 ggcaagctga ccctgaagtt catctgcacc accggcaagc tgcccgtgcc ctggcccacc
     4621 ctcgtgacca ccttcggcta cggcctgaag tgcttcgccc gctaccccga ccacatgaag
     4681 cagcacgact tcttcaagtc cgccatgccc gaaggctacg tccaggagcg caccatcttc
     4741 ttcaaggacg acggcaacta caagacccgc gccgaggtga agttcgaggg cgacaccctg
     4801 gtgaaccgca tcgagctgaa gggcatcgac ttcaaggagg acggcaacat cctggggcac
     4861 aagctggagt acaactacaa cagccacaac gtctatatca tggccgacaa gcagaagaac
     4921 ggcatcaagg tgaacttcaa gatccgccac aacatcgagg acggcagcgt gcagctcgcc
     4981 gaccactacc agcagaacac ccccatcggc gacggccccg tgctgctgcc cgacaaccac
     5041 tacctgagct accagtccaa gctgagcaaa gaccccaacg agaagcgcga tcacatggtc
     5101 ctgctggagt tcgtgaccgc cgccgggatc actggtgaac tcgccggcgt cataaagaga
     5161 ctgtggaagg acagcggtgt gcaagcctgc ttcaacagat cccgggagta ccagctgaac
     5221 gattcggcgg cgtactacct gaatgacttg gacagaatag cacaaccaaa ttacatccca
     5281 acccagcagg atgttctcag aactagagtg aaaacgacgg gaattgtgga aacccacttt
     5341 actttcaaag atcttcattt taaaatgttt gacgtgggag gccagagatc agagcggaag
     5401 aagtggattc actgctttga aggcgtgact gccatcatct tctgtgtggc cctgagtgac
     5461 tatgacctgg ttcttgctga ggatgaagaa atgaaccgga tgcacgaaag catgaagctg
     5521 ttcgatagca tatgtaacaa caagtggttt acggacacat ccatcatcct tttcctgaac
     5581 aagaaggacc tcttcgaaga gaagatcaaa aagagtcccc tcacgatatg ctatccagaa
     5641 tatgcaggct caaacacata tgaagaggcg gctgcgtata tccagtgtca gtttgaagac
     5701 ctcaataaaa ggaaggacac aaaggaaatt tacacccact tcacttgcgc cacggatacg
     5761 aagaatgtgc agtttgtgtt cgatgctgta acggacgtca tcataaagaa taacctaaaa
     5821 gactgtggtc tcttctaagc tggatccacc ggatctagat aactgatcat aatcagccat
     5881 accacatttg tagaggtttt acttgcttta aaaaacctcc cacacctccc cctgaacctg
     5941 aaacataaaa tgaatgcaat tgttgttgtt aacttgttta ttgcagctta taatggttac
     6001 aaataaagca atagcatcac aaatttcaca aataaagcat ttttttcact gcattctagt
     6061 tgtggtttgt ccaaactcat caatgtatct taaggcgtaa attgtaagcg ttaatatttt
     6121 gttaaaattc gcgttaaatt tttgttaaat cagctcattt tttaaccaat aggccgaaat
     6181 cggcaaaatc ccttataaat caaaagaata gaccgagata gggttgagtg ttgttccagt
     6241 ttggaacaag agtccactat taaagaacgt ggactccaac gtcaaagggc gaaaaaccgt
     6301 ctatcagggc gatggcccac tacgtgaacc atcaccctaa tcaagttttt tggggtcgag
     6361 gtgccgtaaa gcactaaatc ggaaccctaa agggagcccc cgatttagag cttgacgggg
     6421 aaagccggcg aacgtggcga gaaaggaagg gaagaaagcg aaaggagcgg gcgctagggc
     6481 gctggcaagt gtagcggtca cgctgcgcgt aaccaccaca cccgccgcgc ttaatgcgcc
     6541 gctacagggc gcgtcaggtg gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt
     6601 atttttctaa atacattcaa atatgtatcc gctcatgaga caataaccct gataaatgct
     6661 tcaataatat tgaaaaagga agagtcctga ggcggaaaga accagctgtg gaatgtgtgt
     6721 cagttagggt gtggaaagtc cccaggctcc ccagcaggca gaagtatgca aagcatgcat
     6781 ctcaattagt cagcaaccag gtgtggaaag tccccaggct ccccagcagg cagaagtatg
     6841 caaagcatgc atctcaatta gtcagcaacc atagtcccgc ccctaactcc gcccatcccg
     6901 cccctaactc cgcccagttc cgcccattct ccgccccatg gctgactaat tttttttatt
     6961 tatgcagagg ccgaggccgc ctcggcctct gagctattcc agaagtagtg aggaggcttt
     7021 tttggaggcc taggcttttg caaagatcga tcaagagaca ggatgaggat cgtttcgcat
     7081 gattgaacaa gatggattgc acgcaggttc tccggccgct tgggtggaga ggctattcgg
     7141 ctatgactgg gcacaacaga caatcggctg ctctgatgcc gccgtgttcc ggctgtcagc
     7201 gcaggggcgc ccggttcttt ttgtcaagac cgacctgtcc ggtgccctga atgaactgca
     7261 agacgaggca gcgcggctat cgtggctggc cacgacgggc gttccttgcg cagctgtgct
     7321 cgacgttgtc actgaagcgg gaagggactg gctgctattg ggcgaagtgc cggggcagga
     7381 tctcctgtca tctcaccttg ctcctgccga gaaagtatcc atcatggctg atgcaatgcg
     7441 gcggctgcat acgcttgatc cggctacctg cccattcgac caccaagcga aacatcgcat
     7501 cgagcgagca cgtactcgga tggaagccgg tcttgtcgat caggatgatc tggacgaaga
     7561 gcatcagggg ctcgcgccag ccgaactgtt cgccaggctc aaggcgagca tgcccgacgg
     7621 cgaggatctc gtcgtgaccc atggcgatgc ctgcttgccg aatatcatgg tggaaaatgg
     7681 ccgcttttct ggattcatcg actgtggccg gctgggtgtg gcggaccgct atcaggacat
     7741 agcgttggct acccgtgata ttgctgaaga gcttggcggc gaatgggctg accgcttcct
     7801 cgtgctttac ggtatcgccg ctcccgattc gcagcgcatc gccttctatc gccttcttga
     7861 cgagttcttc tgagcgggac tctggggttc gaaatgaccg accaagcgac gcccaacctg
     7921 ccatcacgag atttcgattc caccgccgcc ttctatgaaa ggttgggctt cggaatcgtt
     7981 ttccgggacg ccggctggat gatcctccag cgcggggatc tcatgctgga gttcttcgcc
     8041 caccctaggg ggaggctaac tgaaacacgg aaggagacaa taccggaagg aacccgcgct
     8101 atgacggcaa taaaaagaca gaataaaacg cacggtgttg ggtcgtttgt tcataaacgc
     8161 ggggttcggt cccagggctg gcactctgtc gataccccac cgagacccca ttggggccaa
     8221 tacgcccgcg tttcttcctt ttccccaccc caccccccaa gttcgggtga aggcccaggg
     8281 ctcgcagcca acgtcggggc ggcaggccct gccatagcct caggttactc atatatactt
     8341 tagattgatt taaaacttca tttttaattt aaaaggatct aggtgaagat cctttttgat
     8401 aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc agaccccgta
     8461 gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa
     8521 acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt
     8581 tttccgaagg taactggctt cagcagagcg cagataccaa atactgtcct tctagtgtag
     8641 ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta
     8701 atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca
     8761 agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag
     8821 cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga gctatgagaa
     8881 agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga
     8941 acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc
     9001 gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc
     9061 ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg ctggcctttt
     9121 gctcacatgt tctttcctgc gttatcccct gattctgtgg ataaccgtat taccgccatg
     9181 cat
//
