LOCUS       pMD19-PJ23117-sgRNA(dummy)-B0015-SpR_(+BsaI-sites)        3987 bp    DNA     circular UNA 18-AUG-2022
DEFINITION  Base-vector for sgRNA array cloning - flanking BsaI sites.
ACCESSION   urn.local...h-ezr66vj
VERSION     urn.local...h-ezr66vj
KEYWORDS    .
SOURCE      
  ORGANISM  
            .
FEATURES             Location/Qualifiers
     Misc            454..470
                     /created_by="User"
                     /modified_by="User"
                     /label="Universal BsaI-OH"
     misc_feature    460..465
                     /Recognition_pattern="GGTCTC(1/5)"
                     /annotation_group="GGTCTC"
                     /label="BsaI"
                     /note="Geneious type: restriction site"
     promoter        471..507
                     /created_by="User"
                     /modified_by="User"
                     /label="J23117"
     unsure          507..526
                     /created_by="User"
                     /label="sgRNA"
     unsure          507
                     /created_by="User"
                     /label="TSS"
     motif           527..568
                     /created_by="User"
                     /modified_by="User"
                     /label="Cas9 binding"
     terminator      569..609
                     /created_by="User"
                     /modified_by="User"
                     /label="S.pyogenes terminator"
     Misc            610..626
                     /created_by="User"
                     /modified_by="User"
                     /label="Universal BsaI-OH"
     misc_feature    complement(615..620)
                     /Recognition_pattern="GGTCTC(1/5)"
                     /annotation_group="GGTCTC"
                     /label="BsaI"
                     /note="Geneious type: restriction site"
     terminator      634..762
                     /created_by="User"
                     /label="B0015"
     gene            complement(798..1589)
                     /gene="aadA"
                     /note="derived from correct, original source Tn21"
                     /label="aadA gene"
     CDS             complement(798..1589)
                     /gene="aadA"
                     /note="aminoglycoside resistance protein"
                     /codon_start=1
                     /transl_table=11
                     /product="streptomycin 3'-adenylyltransferase"
                     /protein_id="AEP13899.1"
                     /db_xref="GI:347595846"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKP
                     HSDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYP
                     AKRELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQ
                     DLFEALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPA
                     QYQPVILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
                     /label="aadA CDS"
     promoter        complement(1631..1659)
                     /created_by="User"
                     /label="AmpR_promoter"
     ligation        1721..1724
                     /label="Ligation"
     rep_origin      complement(2168..2756)
                     /created_by="User"
                     /label="ori"
     misc_feature    complement(2772..2791)
                     /created_by="User"
                     /modified_by="User"
                     /label
                     /note="Geneious type: primer_bind_reverse"
     CDS             complement(2927..3787)
                     /created_by="User"
                     /label="AmpR"
     misc_feature    3071
                     /Original_Bases="C"
                     /label="C"
                     /note="Geneious type: Editing History Replacement"
ORIGIN      
        1 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca
       61 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg
      121 ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc
      181 accatatgcg gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc atcaggcgcc
      241 attcgccatt caggctgcgc aactgttggg aagggcgatc ggtgcgggcc tcttcgctat
      301 tacgccagct ggcgaaaggg ggatgtgctg caaggcgatt aagttgggta acgccagggt
      361 tttcccagtc acgacgttgt aaaacgacgg ccagtgaatt agaactcggt acgcgcggat
      421 cttccagaga ttgaattcgc ggccgcttct agaacacgtg gtctcggttt ttgacagcta
      481 gctcagtcct agggattgtg ctagctacta tatttttcaa agatgagttt tagagctaga
      541 aatagcaagt taaaataagg ctagtccgtt atcaacttga aaaagtggca ccgagtcggt
      601 gctttttttt tcgcgagacc gacgttacta gagccaggca tcaaataaaa cgaaaggctc
      661 agtcgaaaga ctgggccttt cgttttatct gttgtttgtc ggtgaacgct ctctactaga
      721 gtcacactgg ctcaccttcg ggtgggcctt tctgcgttta tatactagag ccctcggctt
      781 gaacgaattg ttagacatta tttgccgact accttggtga tctcgccttt cacgtagtgg
      841 acaaattctt ccaactgatc tgcgcgcgag gccaagcgat cttcttcttg tccaagataa
      901 gcctgtctag cttcaagtat gacgggctga tactgggccg gcaggcgctc cattgcccag
      961 tcggcagcga catccttcgg cgcgattttg ccggttactg cgctgtacca aatgcgggac
     1021 aacgtaagca ctacatttcg ctcatcgcca gcccagtcgg gcggcgagtt ccatagcgtt
     1081 aaggtttcat ttagcgcctc aaatagatcc tgttcaggaa ccggatcaaa gagttcctcc
     1141 gccgctggac ctaccaaggc aacgctatgt tctcttgctt ttgtcagcaa gatagccaga
     1201 tcaatgtcga tcgtggctgg ctcgaagata cctgcaagaa tgtcattgcg ctgccattct
     1261 ccaaattgca gttcgcgctt agctggataa cgccacggaa tgatgtcgtc gtgcacaaca
     1321 atggtgactt ctacagcgcg gagaatctcg ctctctccag gggaagccga agtttccaaa
     1381 aggtcgttga tcaaagctcg ccgcgttgtt tcatcaagcc ttacggtcac cgtaaccagc
     1441 aaatcaatat cactgtgtgg cttcaggccg ccatccactg cggagccgta caaatgtacg
     1501 gccagcaacg tcggttcgag atggcgctcg atgacgccaa ctacctctga tagttgagtc
     1561 gatacttcgg cgatcaccgc ttccctcata ctcttccttt ttcaatatta ttgaagcatt
     1621 tatcagggtt attgtctcat gagcggatac atatttgaat gtatttagaa aaataaacaa
     1681 atagctagct cactcggtcg ctactactag tagcggccgc tgcagaatcg tcgaacggca
     1741 ggcgtgcaaa cttggcgtaa tcatggtcat agctgtttcc tgtgtgaaat tgttatccgc
     1801 tcacaattcc acacaacata cgagccggaa gcataaagtg taaagcctgg ggtgcctaat
     1861 gagtgagcta actcacatta attgcgttgc gctcactgcc cgctttccag tcgggaaacc
     1921 tgtcgtgcca gctgcattaa tgaatcggcc aacgcgcggg gagaggcggt ttgcgtattg
     1981 ggcgctcttc cgcttcctcg ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag
     2041 cggtatcagc tcactcaaag gcggtaatac ggttatccac agaatcaggg gataacgcag
     2101 gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc
     2161 tggcgttttt ccataggctc cgcccccctg acgagcatca caaaaatcga cgctcaagtc
     2221 agaggtggcg aaacccgaca ggactataaa gataccaggc gtttccccct ggaagctccc
     2281 tcgtgcgctc tcctgttccg accctgccgc ttaccggata cctgtccgcc tttctccctt
     2341 cgggaagcgt ggcgctttct catagctcac gctgtaggta tctcagttcg gtgtaggtcg
     2401 ttcgctccaa gctgggctgt gtgcacgaac cccccgttca gcccgaccgc tgcgccttat
     2461 ccggtaacta tcgtcttgag tccaacccgg taagacacga cttatcgcca ctggcagcag
     2521 ccactggtaa caggattagc agagcgaggt atgtaggcgg tgctacagag ttcttgaagt
     2581 ggtggcctaa ctacggctac actagaagaa cagtatttgg tatctgcgct ctgctgaagc
     2641 cagttacctt cggaaaaaga gttggtagct cttgatccgg caaacaaacc accgctggta
     2701 gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag
     2761 atcctttgat cttttctacg gggtctgacg ctcagtggaa cgaaaactca cgttaaggga
     2821 ttttggtcat gagattatca aaaaggatct tcacctagat ccttttaaat taaaaatgaa
     2881 gttttaaatc aatctaaagt atatatgagt aaacttggtc tgacagttac caatgcttaa
     2941 tcagtgaggc acctatctca gcgatctgtc tatttcgttc atccatagtt gcctgactcc
     3001 ccgtcgtgta gataactacg atacgggagg gcttaccatc tggccccagt gctgcaatga
     3061 taccgcgaga tccacgctca ccggctccag atttatcagc aataaaccag ccagccggaa
     3121 gggccgagcg cagaagtggt cctgcaactt tatccgcctc catccagtct attaattgtt
     3181 gccgggaagc tagagtaagt agttcgccag ttaatagttt gcgcaacgtt gttgccattg
     3241 ctacaggcat cgtggtgtca cgctcgtcgt ttggtatggc ttcattcagc tccggttccc
     3301 aacgatcaag gcgagttaca tgatccccca tgttgtgcaa aaaagcggtt agctccttcg
     3361 gtcctccgat cgttgtcaga agtaagttgg ccgcagtgtt atcactcatg gttatggcag
     3421 cactgcataa ttctcttact gtcatgccat ccgtaagatg cttttctgtg actggtgagt
     3481 actcaaccaa gtcattctga gaatagtgta tgcggcgacc gagttgctct tgcccggcgt
     3541 caatacggga taataccgcg ccacatagca gaactttaaa agtgctcatc attggaaaac
     3601 gttcttcggg gcgaaaactc tcaaggatct taccgctgtt gagatccagt tcgatgtaac
     3661 ccactcgtgc acccaactga tcttcagcat cttttacttt caccagcgtt tctgggtgag
     3721 caaaaacagg aaggcaaaat gccgcaaaaa agggaataag ggcgacacgg aaatgttgaa
     3781 tactcatact cttccttttt caatattatt gaagcattta tcagggttat tgtctcatga
     3841 gcggatacat atttgaatgt atttagaaaa ataaacaaat gggggttccg cgcacatttc
     3901 cccgaaaagt gccacctgac gtctaagaaa ccattattat catgacatta acctataaaa
     3961 ataggcgtat cacgaggccc tttcgtc
//
