LOCUS       Exported               10840 bp ds-DNA     circular SYN 22-AUG-2022
DEFINITION  synthetic DNA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10840)
  TITLE     NEBuilder-generated Construct
  JOURNAL   Exported  3-JUL-2019 from NEBuilder https://nebuilder.neb.com
REFERENCE   2  (bases 1 to 10840)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Monday, Aug 22, 2022 from SnapGene 5.0.8
            https://www.snapgene.com
COMMENT     NEBuilder-generated oligos (UPPERCASE = gene-specific, lowercase = 
            overlap)
            APOE E3_fwd: 
            gattaattaagctagctaatATTAAGCTAGTAATTCGATATCAAGCTTATCGATAATCAACC
            APOE E3_fwd 3'Tm: 67.6  3'Ta: 68.6
            APOE E3_rev: AGCCATCTCTAGAGGGCCGG
            APOE E3_rev 3'Tm: 71.5  3'Ta: 68.6
            PuroR_fwd: ccggccctctagagatggctATGACCGAGTACAAGCCCAC
            PuroR_fwd 3'Tm: 67.1  3'Ta: 63.2
            PuroR_rev: ATTAGCTAGCTTAATTAATCAGGCAC
            PuroR_rev 3'Tm: 62.2  3'Ta: 63.2
FEATURES             Location/Qualifiers
     source          1..10840
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     source          join(2877..10840,1..2876)
                     /organism="synthetic DNA construct"
                     /plasmid="FUGW APOE E3 PuroR"
                     /mol_type="other DNA"
     promoter        1..1212
                     /label=UbC promoter
                     /note="human ubiquitin C promoter"
     primer_bind     1216..1260
                     /label=APOE_fwd
                     /note="APOE_fwd"
                     /note="gene-specific Tm: 64.9  Ta: 65.9"
                     /note="overlap: gcttgggctgcaggtcgact"
                     /note="spacer: accacc"
                     /note="gene-specific primer: ATGAAGGTTCTGTGGGCTG"
     gene            1236..2250
                     /label=APOE
                     /note="APOE"
     sig_peptide     1242..1295
                     /gene="APOE"
                     /gene_synonym="AD2; APO-E; LDLCQ5; LPG"
                     /inference="COORDINATES:same species:SignalP"
     exon            1285..1477
                     /gene="APOE"
                     /gene_synonym="AD2; APO-E; LDLCQ5; LPG"
                     /inference="alignment:same species:1.39.8"
     CDS             1296..2192
                     /codon_start=1
                     /gene="APOE"
                     /gene_synonym="AD2; APO-E; LDLCQ5; LPG"
                     /product="Apolipoprotein E"
                     /label=APOE
                     /note="propagated from UniProtKB/Swiss-Prot (P02649.1)"
                     /experiment="experimental evidence, no additional details 
                     recorded"
                     /translation="KVEQAVETEPEPELRQQTEWQSGQRWELALGRFWDYLRWVQTLSE
                     QVQEELLSSQVTQELRALMDETMKELKAYKSELEEQLTPVAEETRARLSKELQAAQARL
                     GADMEDVCGRLVQYRGEVQAMLGQSTEELRVRLASHLRKLRKRLLRDADDLQKRLAVYQ
                     AGAREGAERGLSAIRERLGPLVEQGRVRAATVGSLAGQPLQERAQAWGERLRARMEEMG
                     SRTRDRLDEVKEQVAEVRAKLEEQAQQIRLQAEAFQARLKSWFEPLVEDMQRQWAGLVE
                     KVQAAVGTSAAPVPSDNH"
     STS             1407..1514
                     /gene="APOE"
                     /gene_synonym="AD2; APO-E; LDLCQ5; LPG"
                     /standard_name="PMC99927P2"
                     /label=PMC99927P2
                     /db_xref="UniSTS:273705"
     misc_feature    1479..2006
                     /gene="APOE"
                     /gene_synonym="AD2; APO-E; LDLCQ5; LPG"
                     /note="propagated from UniProtKB/Swiss-Prot (P02649.1); 
                     Region: 8 X 22 AA approximate tandem repeats"
                     /experiment="experimental evidence, no additional details 
                     recorded"
     STS             1500..1798
                     /gene="APOE"
                     /gene_synonym="AD2; APO-E; LDLCQ5; LPG"
                     /standard_name="GDB:171177"
                     /label=GDB:171177
                     /db_xref="UniSTS:154776"
     STS             1533..1798
                     /gene="APOE"
                     /gene_synonym="AD2; APO-E; LDLCQ5; LPG"
                     /standard_name="GDB:181693"
                     /label=GDB:181693
                     /db_xref="UniSTS:155329"
     misc_feature    1551..2138
                     /gene="APOE"
                     /gene_synonym="AD2; APO-E; LDLCQ5; LPG"
                     /note="Apolipoprotein A1/A4/E domain; Region: 
                     Apolipoprotein; pfam01442"
                     /db_xref="CDD:250625"
     STS             1562..1789
                     /gene="APOE"
                     /gene_synonym="AD2; APO-E; LDLCQ5; LPG"
                     /standard_name="GDB:169570"
                     /label=GDB:169570
                     /db_xref="UniSTS:154765"
     STS             1565..1790
                     /gene="APOE"
                     /gene_synonym="AD2; APO-E; LDLCQ5; LPG"
                     /standard_name="GDB:438097"
                     /label=GDB:438097
                     /db_xref="UniSTS:157265"
     STS             1566..1836
                     /gene="APOE"
                     /gene_synonym="AD2; APO-E; LDLCQ5; LPG"
                     /standard_name="GDB:196979"
                     /label=GDB:196979
                     /db_xref="UniSTS:155901"
     STS             1575..1904
                     /gene="APOE"
                     /gene_synonym="AD2; APO-E; LDLCQ5; LPG"
                     /standard_name="GDB:177380"
                     /label=GDB:177380
                     /db_xref="UniSTS:154875"
     STS             1575..1792
                     /gene="APOE"
                     /gene_synonym="AD2; APO-E; LDLCQ5; LPG"
                     /standard_name="PMC117303P1"
                     /label=PMC117303P1
                     /db_xref="UniSTS:270328"
     misc_feature    1611..1630
                     /label=CRISPR9
     misc_feature    1619..1638
                     /label=CRISPR10
     variation       1629
                     /label=rs429358
     misc_feature    1713..1745
                     /gene="APOE"
                     /gene_synonym="AD2; APO-E; LDLCQ5; LPG"
                     /label=LDL Receptor Binding
                     /note="propagated from UniProtKB/Swiss-Prot (P02649.1); 
                     Region: LDL receptor binding. {ECO:0000255}"
                     /experiment="experimental evidence, no additional details 
                     recorded"
     misc_feature    1725..1736
                     /gene="APOE"
                     /gene_synonym="AD2; APO-E; LDLCQ5; LPG"
                     /note="propagated from UniProtKB/Swiss-Prot (P02649.1); 
                     Region: Heparin-binding"
                     /experiment="experimental evidence, no additional details 
                     recorded"
     STS             1767..1910
                     /gene="APOE"
                     /gene_synonym="AD2; APO-E; LDLCQ5; LPG"
                     /standard_name="STS-N29699"
                     /label=STS-N29699
                     /db_xref="UniSTS:34644"
     variation       1767
                     /label=rs7412
     misc_feature    1794..1850
                     /label=Hinge Region
     STS             1894..2094
                     /gene="APOE"
                     /gene_synonym="AD2; APO-E; LDLCQ5; LPG"
                     /standard_name="PMC310963P4"
                     /label=PMC310963P4
                     /db_xref="UniSTS:272827"
     misc_feature    1926..1949
                     /gene="APOE"
                     /gene_synonym="AD2; APO-E; LDLCQ5; LPG"
                     /note="propagated from UniProtKB/Swiss-Prot (P02649.1); 
                     Region: Heparin-binding"
                     /experiment="experimental evidence, no additional details 
                     recorded"
     STS             1981..2112
                     /gene="APOE"
                     /gene_synonym="AD2; APO-E; LDLCQ5; LPG"
                     /standard_name="RH11470"
                     /label=RH11470
                     /db_xref="UniSTS:72925"
     misc_feature    2025..2111
                     /label=Lipid Binding
     CDS             2193..2246
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     primer_bind     2239..2278
                     /label=PuroR_fwd
                     /note="PuroR_fwd"
                     /note="gene-specific Tm: 67.1  Ta: 63.2"
                     /note="overlap: ccggccctctagagatggct"
                     /note="gene-specific primer: ATGACCGAGTACAAGCCCAC"
     primer_bind     complement(2239..2258)
                     /label=APOE E3_rev
                     /note="APOE E3_rev"
                     /note="gene-specific Tm: 71.5  Ta: 68.6"
                     /note="gene-specific primer: AGCCATCTCTAGAGGGCCGG"
     gene            2259..2876
                     /label=PuroR
                     /note="PuroR"
     CDS             2259..2858
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(2851..2877)
                     /label=PuroR_rev
                     /note="PuroR_rev"
                     /note="gene-specific Tm: 62.2  Ta: 63.2"
                     /note="gene-specific primer: ATTAGCTAGCTTAATTAATCAGGCAC"
     primer_bind     2857..2918
                     /label=APOE E3_fwd
                     /note="APOE E3_fwd"
                     /note="gene-specific Tm: 67.6  Ta: 68.6"
                     /note="overlap: gattaattaagctagctaat"
                     /note="gene-specific primer: 
                     ATTAAGCTAGTAATTCGATATCAAGCTTATCGATAATCAACC"
     gene            join(2877..10840,1..2258)
                     /label=APOE E3
                     /note="APOE E3"
     gene            join(2888..10840,1..2246)
                     /label=FUGW-APOE
                     /note="FUGW-APOE"
     gene            join(2888..10840,1..1235)
                     /label=FUGW
                     /note="FUGW"
     misc_feature    2911..3499
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     CDS             complement(3382..3393)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(3502..3518)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     LTR             4024..4204
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     polyA_signal    4236..4460
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      4506..4934
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     rep_origin      4506..4864
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     promoter        4948..5277
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      5128..5263
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     promoter        5325..5372
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             5391..5765
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    5895..6016
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6065..6081)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    6089..6105
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(6113..6143)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6158..6179
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     rep_origin      complement(6467..7055)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(7226..8086)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(8087..8191)
                     /gene="bla"
                     /label=AmpR promoter
     enhancer        8457..8836
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        8837..9040
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     LTR             9054..9234
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    9281..9406
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    9899..10132
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     misc_feature    10659..10776
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
ORIGIN
        1 ggcctccgcg ccgggttttg gcgcctcccg cgggcgcccc cctcctcacg gcgagcgctg
       61 ccacgtcaga cgaagggcgc agcgagcgtc ctgatccttc cgcccggacg ctcaggacag
      121 cggcccgctg ctcataagac tcggccttag aaccccagta tcagcagaag gacattttag
      181 gacgggactt gggtgactct agggcactgg ttttctttcc agagagcgga acaggcgagg
      241 aaaagtagtc ccttctcggc gattctgcgg agggatctcc gtggggcggt gaacgccgat
      301 gattatataa ggacgcgccg ggtgtggcac agctagttcc gtcgcagccg ggatttgggt
      361 cgcggttctt gtttgtggat cgctgtgatc gtcacttggt gagtagcggg ctgctgggct
      421 ggccggggct ttcgtggccg ccgggccgct cggtgggacg gaagcgtgtg gagagaccgc
      481 caagggctgt agtctgggtc cgcgagcaag gttgccctga actgggggtt ggggggagcg
      541 cagcaaaatg gcggctgttc ccgagtcttg aatggaagac gcttgtgagg cgggctgtga
      601 ggtcgttgaa acaaggtggg gggcatggtg ggcggcaaga acccaaggtc ttgaggcctt
      661 cgctaatgcg ggaaagctct tattcgggtg agatgggctg gggcaccatc tggggaccct
      721 gacgtgaagt ttgtcactga ctggagaact cggtttgtcg tctgttgcgg gggcggcagt
      781 tatggcggtg ccgttgggca gtgcacccgt acctttggga gcgcgcgccc tcgtcgtgtc
      841 gtgacgtcac ccgttctgtt ggcttataat gcagggtggg gccacctgcc ggtaggtgtg
      901 cggtaggctt ttctccgtcg caggacgcag ggttcgggcc tagggtaggc tctcctgaat
      961 cgacaggcgc cggacctctg gtgaggggag ggataagtga ggcgtcagtt tctttggtcg
     1021 gttttatgta cctatcttct taagtagctg aagctccggt tttgaactat gcgctcgggg
     1081 ttggcgagtg tgttttgtga agttttttag gcaccttttg aaatgtaatc atttgggtca
     1141 atatgtaatt ttcagtgtta gactagtaaa ttgtccgcta aattctggcc gtttttggct
     1201 tttttgttag acgaagcttg ggctgcaggt cgactaccac catgaaggtt ctgtgggctg
     1261 cgttgctggt cacattcctg gcaggatgcc aggccaaggt ggagcaagcg gtggagacag
     1321 agccggagcc cgagctgcgc cagcagaccg agtggcagag cggccagcgc tgggaactgg
     1381 cactgggtcg cttttgggat tacctgcgct gggtgcagac actgtctgag caggtgcagg
     1441 aggagctgct cagctcccag gtcacccagg aactgagggc gctgatggac gagaccatga
     1501 aggagttgaa ggcctacaaa tcggaactgg aggaacaact gaccccggtg gcggaggaga
     1561 cgcgggcacg gctgtccaag gagctgcagg cggcgcaggc ccggctgggc gcggacatgg
     1621 aggacgtgcg cggccgcctg gtgcagtacc gcggcgaggt gcaggccatg ctcggccaga
     1681 gcaccgagga gctgcgggtg cgcctcgcct cccacctgcg caagctgcgt aagcggctcc
     1741 tccgcgatgc cgatgacctg cagaagcgcc tggcagtgta ccaggccggg gcccgcgagg
     1801 gcgccgagcg cggcctcagc gccatccgcg agcgcctggg gcccctggtg gaacagggcc
     1861 gcgtgcgggc cgccactgtg ggctccctgg ccggccagcc gctacaggag cgggcccagg
     1921 cctggggcga gcggctgcgc gcgcggatgg aggagatggg cagccggacc cgcgaccgcc
     1981 tggacgaggt gaaggagcag gtggcggagg tgcgcgccaa gctggaggag caggcccagc
     2041 agatacgcct gcaggccgag gccttccagg cccgcctcaa gagctggttc gagcccctgg
     2101 tggaagacat gcagcgccag tgggccgggc tggtggagaa ggtgcaggct gccgtgggca
     2161 ccagcgccgc ccctgtgccc agcgacaatc acgagggcag gggaagtctt ctaacatgcg
     2221 gggacgtgga ggaaaatccc ggccctctag agatggctat gaccgagtac aagcccacgg
     2281 tgcgcctcgc cacccgcgac gacgtcccca gggccgtacg caccctcgcc gccgcgttcg
     2341 ccgactaccc cgccacgcgc cacaccgtcg atccggaccg ccacatcgag cgggtcaccg
     2401 agctgcaaga actcttcctc acgcgcgtcg ggctcgacat cggcaaggtg tgggtcgcgg
     2461 acgacggcgc cgcggtggcg gtctggacca cgccggagag cgtcgaagcg ggggcggtgt
     2521 tcgccgagat cggcccgcgc atggccgagt tgagcggttc ccggctggcc gcgcagcaac
     2581 agatggaagg cctcctggcg ccgcaccggc ccaaggagcc cgcgtggttc ctggccaccg
     2641 tcggcgtctc gcccgaccac cagggcaagg gtctgggcag cgccgtcgtg ctccccggag
     2701 tggaggcggc cgagcgcgcc ggggtgcccg ccttcctgga gacctccgcg ccccgcaacc
     2761 tccccttcta cgagcggctc ggcttcaccg tcaccgccga cgtcgaggtg cccgaaggac
     2821 cgcgcacctg gtgcatgacc cgcaagcccg gtgcctgatt aattaagcta gctaatatta
     2881 agctagtaat tcgatatcaa gcttatcgat aatcaacctc tggattacaa aatttgtgaa
     2941 agattgactg gtattcttaa ctatgttgct ccttttacgc tatgtggata cgctgcttta
     3001 atgcctttgt atcatgctat tgcttcccgt atggctttca ttttctcctc cttgtataaa
     3061 tcctggttgc tgtctcttta tgaggagttg tggcccgttg tcaggcaacg tggcgtggtg
     3121 tgcactgtgt ttgctgacgc aacccccact ggttggggca ttgccaccac ctgtcagctc
     3181 ctttccggga ctttcgcttt ccccctccct attgccacgg cggaactcat cgccgcctgc
     3241 cttgcccgct gctggacagg ggctcggctg ttgggcactg acaattccgt ggtgttgtcg
     3301 gggaaatcat cgtcctttcc ttggctgctc gcctgtgttg ccacctggat tctgcgcggg
     3361 acgtccttct gctacgtccc ttcggccctc aatccagcgg accttccttc ccgcggcctg
     3421 ctgccggctc tgcggcctct tccgcgtctt cgccttcgcc ctcagacgag tcggatctcc
     3481 ctttgggccg cctccccgca tcgataccgt cgacctcgag acctagaaaa acatggagca
     3541 atcacaagta gcaatacagc agctaccaat gctgattgtg cctggctaga agcacaagag
     3601 gaggaggagg tgggttttcc agtcacacct caggtacctt taagaccaat gacttacaag
     3661 gcagctgtag atcttagcca ctttttaaaa gaaaaggggg gactggaagg gctaattcac
     3721 tcccaacgaa gacaagatat ccttgatctg tggatctacc acacacaagg ctacttccct
     3781 gattggcaga actacacacc agggccaggg atcagatatc cactgacctt tggatggtgc
     3841 tacaagctag taccagttga gcaagagaag gtagaagaag ccaatgaagg agagaacacc
     3901 cgcttgttac accctgtgag cctgcatggg atggatgacc cggagagaga agtattagag
     3961 tggaggtttg acagccgcct agcatttcat cacatggccc gagagctgca tccggactgt
     4021 actgggtctc tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac
     4081 ccactgctta agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg
     4141 ttgtgtgact ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct
     4201 agcagggccc gtttaaaccc gctgatcagc ctcgactgtg ccttctagtt gccagccatc
     4261 tgttgtttgc ccctcccccg tgccttcctt gaccctggaa ggtgccactc ccactgtcct
     4321 ttcctaataa aatgaggaaa ttgcatcgca ttgtctgagt aggtgtcatt ctattctggg
     4381 gggtggggtg gggcaggaca gcaaggggga ggattgggaa gacaatagca ggcatgctgg
     4441 ggatgcggtg ggctctatgg cttctgaggc ggaaagaacc agctggggct ctagggggta
     4501 tccccacgcg ccctgtagcg gcgcattaag cgcggcgggt gtggtggtta cgcgcagcgt
     4561 gaccgctaca cttgccagcg ccctagcgcc cgctcctttc gctttcttcc cttcctttct
     4621 cgccacgttc gccggctttc cccgtcaagc tctaaatcgg gggctccctt tagggttccg
     4681 atttagtgct ttacggcacc tcgaccccaa aaaacttgat tagggtgatg gttcacgtag
     4741 tgggccatcg ccctgataga cggtttttcg ccctttgacg ttggagtcca cgttctttaa
     4801 tagtggactc ttgttccaaa ctggaacaac actcaaccct atctcggtct attcttttga
     4861 tttataaggg attttgccga tttcggccta ttggttaaaa aatgagctga tttaacaaaa
     4921 atttaacgcg aattaattct gtggaatgtg tgtcagttag ggtgtggaaa gtccccaggc
     4981 tccccagcag gcagaagtat gcaaagcatg catctcaatt agtcagcaac caggtgtgga
     5041 aagtccccag gctccccagc aggcagaagt atgcaaagca tgcatctcaa ttagtcagca
     5101 accatagtcc cgcccctaac tccgcccatc ccgcccctaa ctccgcccag ttccgcccat
     5161 tctccgcccc atggctgact aatttttttt atttatgcag aggccgaggc cgcctctgcc
     5221 tctgagctat tccagaagta gtgaggaggc ttttttggag gcctaggctt ttgcaaaaag
     5281 ctcccgggag cttgtatatc cattttcgga tctgatcagc acgtgttgac aattaatcat
     5341 cggcatagta tatcggcata gtataatacg acaaggtgag gaactaaacc atggccaagt
     5401 tgaccagtgc cgttccggtg ctcaccgcgc gcgacgtcgc cggagcggtc gagttctgga
     5461 ccgaccggct cgggttctcc cgggacttcg tggaggacga cttcgccggt gtggtccggg
     5521 acgacgtgac cctgttcatc agcgcggtcc aggaccaggt ggtgccggac aacaccctgg
     5581 cctgggtgtg ggtgcgcggc ctggacgagc tgtacgccga gtggtcggag gtcgtgtcca
     5641 cgaacttccg ggacgcctcc gggccggcca tgaccgagat cggcgagcag ccgtgggggc
     5701 gggagttcgc cctgcgcgac ccggccggca actgcgtgca cttcgtggcc gaggagcagg
     5761 actgacacgt gctacgagat ttcgattcca ccgccgcctt ctatgaaagg ttgggcttcg
     5821 gaatcgtttt ccgggacgcc ggctggatga tcctccagcg cggggatctc atgctggagt
     5881 tcttcgccca ccccaacttg tttattgcag cttataatgg ttacaaataa agcaatagca
     5941 tcacaaattt cacaaataaa gcattttttt cactgcattc tagttgtggt ttgtccaaac
     6001 tcatcaatgt atcttatcat gtctgtatac cgtcgacctc tagctagagc ttggcgtaat
     6061 catggtcata gctgtttcct gtgtgaaatt gttatccgct cacaattcca cacaacatac
     6121 gagccggaag cataaagtgt aaagcctggg gtgcctaatg agtgagctaa ctcacattaa
     6181 ttgcgttgcg ctcactgccc gctttccagt cgggaaacct gtcgtgccag ctgcattaat
     6241 gaatcggcca acgcgcgggg agaggcggtt tgcgtattgg gcgctcttcc gcttcctcgc
     6301 tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg
     6361 cggtaatacg gttatccaca gaatcagggg ataacgcagg aaagaacatg tgagcaaaag
     6421 gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc
     6481 gcccccctga cgagcatcac aaaaatcgac gctcaagtca gaggtggcga aacccgacag
     6541 gactataaag ataccaggcg tttccccctg gaagctccct cgtgcgctct cctgttccga
     6601 ccctgccgct taccggatac ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc
     6661 atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg
     6721 tgcacgaacc ccccgttcag cccgaccgct gcgccttatc cggtaactat cgtcttgagt
     6781 ccaacccggt aagacacgac ttatcgccac tggcagcagc cactggtaac aggattagca
     6841 gagcgaggta tgtaggcggt gctacagagt tcttgaagtg gtggcctaac tacggctaca
     6901 ctagaagaac agtatttggt atctgcgctc tgctgaagcc agttaccttc ggaaaaagag
     6961 ttggtagctc ttgatccggc aaacaaacca ccgctggtag cggtggtttt tttgtttgca
     7021 agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg
     7081 ggtctgacgc tcagtggaac gaaaactcac gttaagggat tttggtcatg agattatcaa
     7141 aaaggatctt cacctagatc cttttaaatt aaaaatgaag ttttaaatca atctaaagta
     7201 tatatgagta aacttggtct gacagttacc aatgcttaat cagtgaggca cctatctcag
     7261 cgatctgtct atttcgttca tccatagttg cctgactccc cgtcgtgtag ataactacga
     7321 tacgggaggg cttaccatct ggccccagtg ctgcaatgat accgcgagac ccacgctcac
     7381 cggctccaga tttatcagca ataaaccagc cagccggaag ggccgagcgc agaagtggtc
     7441 ctgcaacttt atccgcctcc atccagtcta ttaattgttg ccgggaagct agagtaagta
     7501 gttcgccagt taatagtttg cgcaacgttg ttgccattgc tacaggcatc gtggtgtcac
     7561 gctcgtcgtt tggtatggct tcattcagct ccggttccca acgatcaagg cgagttacat
     7621 gatcccccat gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc gttgtcagaa
     7681 gtaagttggc cgcagtgtta tcactcatgg ttatggcagc actgcataat tctcttactg
     7741 tcatgccatc cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag tcattctgag
     7801 aatagtgtat gcggcgaccg agttgctctt gcccggcgtc aatacgggat aataccgcgc
     7861 cacatagcag aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct
     7921 caaggatctt accgctgttg agatccagtt cgatgtaacc cactcgtgca cccaactgat
     7981 cttcagcatc ttttactttc accagcgttt ctgggtgagc aaaaacagga aggcaaaatg
     8041 ccgcaaaaaa gggaataagg gcgacacgga aatgttgaat actcatactc ttcctttttc
     8101 aatattattg aagcatttat cagggttatt gtctcatgag cggatacata tttgaatgta
     8161 tttagaaaaa taaacaaata ggggttccgc gcacatttcc ccgaaaagtg ccacctgacg
     8221 tcgacggatc gggagatctc ccgatcccct atggtgcact ctcagtacaa tctgctctga
     8281 tgccgcatag ttaagccagt atctgctccc tgcttgtgtg ttggaggtcg ctgagtagtg
     8341 cgcgagcaaa atttaagcta caacaaggca aggcttgacc gacaattgca tgaagaatct
     8401 gcttagggtt aggcgttttg cgctgcttcg cgatgtacgg gccagatata cgcgttgaca
     8461 ttgattattg actagttatt aatagtaatc aattacgggg tcattagttc atagcccata
     8521 tatggagttc cgcgttacat aacttacggt aaatggcccg cctggctgac cgcccaacga
     8581 cccccgccca ttgacgtcaa taatgacgta tgttcccata gtaacgccaa tagggacttt
     8641 ccattgacgt caatgggtgg agtatttacg gtaaactgcc cacttggcag tacatcaagt
     8701 gtatcatatg ccaagtacgc cccctattga cgtcaatgac ggtaaatggc ccgcctggca
     8761 ttatgcccag tacatgacct tatgggactt tcctacttgg cagtacatct acgtattagt
     8821 catcgctatt accatggtga tgcggttttg gcagtacatc aatgggcgtg gatagcggtt
     8881 tgactcacgg ggatttccaa gtctccaccc cattgacgtc aatgggagtt tgttttggca
     8941 ccaaaatcaa cgggactttc caaaatgtcg taacaactcc gccccattga cgcaaatggg
     9001 cggtaggcgt gtacggtggg aggtctatat aagcagcgcg ttttgcctgt actgggtctc
     9061 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta
     9121 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact
     9181 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg
     9241 cccgaacagg gacttgaaag cgaaagggaa accagaggag ctctctcgac gcaggactcg
     9301 gcttgctgaa gcgcgcacgg caagaggcga ggggcggcga ctggtgagta cgccaaaaat
     9361 tttgactagc ggaggctaga aggagagaga tgggtgcgag agcgtcagta ttaagcgggg
     9421 gagaattaga tcgcgatggg aaaaaattcg gttaaggcca gggggaaaga aaaaatataa
     9481 attaaaacat atagtatggg caagcaggga gctagaacga ttcgcagtta atcctggcct
     9541 gttagaaaca tcagaaggct gtagacaaat actgggacag ctacaaccat cccttcagac
     9601 aggatcagaa gaacttagat cattatataa tacagtagca accctctatt gtgtgcatca
     9661 aaggatagag ataaaagaca ccaaggaagc tttagacaag atagaggaag agcaaaacaa
     9721 aagtaagacc accgcacagc aagcggccgc tgatcttcag acctggagga ggagatatga
     9781 gggacaattg gagaagtgaa ttatataaat ataaagtagt aaaaattgaa ccattaggag
     9841 tagcacccac caaggcaaag agaagagtgg tgcagagaga aaaaagagca gtgggaatag
     9901 gagctttgtt ccttgggttc ttgggagcag caggaagcac tatgggcgca gcgtcaatga
     9961 cgctgacggt acaggccaga caattattgt ctggtatagt gcagcagcag aacaatttgc
    10021 tgagggctat tgaggcgcaa cagcatctgt tgcaactcac agtctggggc atcaagcagc
    10081 tccaggcaag aatcctggct gtggaaagat acctaaagga tcaacagctc ctggggattt
    10141 ggggttgctc tggaaaactc atttgcacca ctgctgtgcc ttggaatgct agttggagta
    10201 ataaatctct ggaacagatt tggaatcaca cgacctggat ggagtgggac agagaaatta
    10261 acaattacac aagcttaata cactccttaa ttgaagaatc gcaaaaccag caagaaaaga
    10321 atgaacaaga attattggaa ttagataaat gggcaagttt gtggaattgg tttaacataa
    10381 caaattggct gtggtatata aaattattca taatgatagt aggaggcttg gtaggtttaa
    10441 gaatagtttt tgctgtactt tctatagtga atagagttag gcagggatat tcaccattat
    10501 cgtttcagac ccacctccca accccgaggg gacccgacag gcccgaagga atagaagaag
    10561 aaggtggaga gagagacaga gacagatcca ttcgattagt gaacggatcg gcactgcgtg
    10621 cgccaattct gcagacaaat ggcagtattc atccacaatt ttaaaagaaa aggggggatt
    10681 ggggggtaca gtgcagggga aagaatagta gacataatag caacagacat acaaactaaa
    10741 gaattacaaa aacaaattac aaaaattcaa aattttcggg tttattacag ggacagcaga
    10801 gatccagttt ggttaattaa cccgtgtcgg ctccagatct
//
