LOCUS       Exported               10013 bp ds-DNA     circular SYN 22-AUG-2022
DEFINITION  synthetic circular DNA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10013)
  AUTHORS   .
  TITLE     NEBuilder-generated Construct
  JOURNAL   Exported  13-OCT-2017 from NEBuilder http://nebuilder.neb.com
REFERENCE   2  (bases 1 to 10013)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Monday, Aug 22, 2022 from SnapGene 5.0.8
            https://www.snapgene.com
COMMENT     NEBuilder-generated oligos (UPPERCASE = gene-specific, lowercase = 
            overlap)
            APOE-Fwd: gaccgatccagcctccgcggccccggccaccATGAAGGTTCTGTGGGCTG
            APOE-Fwd Tm: 64.9  Ta: 65.9
            APOE-Rev: tgttagaagacttcccctgccctctGTGATTGTCGCTGGGCAC
            APOE-Rev Tm: 67.0  Ta: 65.9
            Vector backbone digested with EcoRI and XbaI
FEATURES             Location/Qualifiers
     source          1..10013
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     source          437..1393
                     /organism="synthetic DNA construct
                     /mol_type=""other DNA
                     /note=""APOE"
     source          1394..5920
                     /organism="synthetic DNA construct
                     /mol_type=""other DNA
                     /note=""pTet-O-Ngn2-Puro"
     source          join(5921..10013,1..5920)
                     /organism="synthetic DNA construct"
                     /plasmid="NEBuilder_Assembly"
                     /mol_type="other DNA"
     source          join(5921..10013,1..436)
                     /organism="synthetic DNA construct
                     /mol_type=""other DNA
                     /note=""pTet-O-Ngn2-Puro"
     protein_bind    1..271
                     /label=tetracycline response element
                     /bound_moiety="tetracycline repressor TetR"
                     /note="contains seven copies of the tetracycline operator 
                     tetO"
     promoter        304..342
                     /label=minimal CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     regulatory      436..442
                     /regulatory_class="ribosome_binding_site"
                     /label=Kozak sequence
     CDS             443..1393
                     /codon_start=1
                     /label=APOE
                     /translation="MKVLWAALLVTFLAGCQAKVEQAVETEPEPELRQQTEWQSGQRWE
                     LALGRFWDYLRWVQTLSEQVQEELLSSQVTQELRALMDETMKELKAYKSELEEQLTPVA
                     EETRARLSKELQAAQARLGADMEDVCGRLVQYRGEVQAMLGQSTEELRVRLASHLRKLR
                     KRLLRDADDLQKRLAVYQAGAREGAERGLSAIRERLGPLVEQGRVRAATVGSLAGQPLQ
                     ERAQAWGERLRARMEEMGSRTRDRLDEVKEQVAEVRAKLEEQAQQIRLQAEAFQARLKS
                     WFEPLVEDMQRQWAGLVEKVQAAVGTSAAPVPSDNH"
     variation       830
                     /label=rs429358
                     /note="E2 = T; E3 = T; E4 = C; Cys (TGC)->Arg (CGC)"
     variation       968
                     /label=rs7412
                     /note="E2 = T; E3 = C; E4 = C"
     CDS             1394..1447
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     misc_structure  1418..1444
                     /label=T2A antibody epitope
                     /note="http://www.emdmillipore.com/US/en/product/Anti-2A-pe
                     ptide-Antibody-clone-3H4,MM_NF-MABS2005"
     CDS             1454..2053
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     misc_feature    2092..2680
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     CDS             complement(2563..2574)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(2683..2699)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     LTR             3205..3385
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     polyA_signal    3417..3641
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      3687..4115
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     promoter        4129..4458
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      4309..4444
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     promoter        4506..4553
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             4572..4946
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    5076..5197
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5246..5262)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    5270..5286
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(5294..5324)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    5339..5360
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     rep_origin      complement(5648..6236)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(5921..5962)
                     /label=APOE-Rev
                     /note="APOE-Rev
                     gene-specific Tm: 67.0  Ta: 65.9
                     overlap: tgttagaagacttcccctgccctct
                     gene-specific primer: GTGATTGTCGCTGGGCAC"
     primer_bind     5921..5938
                     /label=APOE-Fwd
                     /note="APOE-Fwd
                     gene-specific Tm: 64.9  Ta: 65.9
                     overlap: gaccgatccagcctccgcggccccg
                     spacer: gccacc
                     gene-specific primer: ATGAAGGTTCTGTGGGCTG"
     CDS             complement(6407..7267)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(7268..7372)
                     /gene="bla"
                     /label=AmpR promoter
     enhancer        7638..8017
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        8019..8217
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     LTR             8235..8415
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    8462..8587
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    9080..9313
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     misc_feature    9840..9957
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
ORIGIN
        1 tccctatcag tgatagagaa aagtgaaagt cgagtttacc actccctatc agtgatagag
       61 aaaagtgaaa gtcgagttta ccactcccta tcagtgatag agaaaagtga aagtcgagtt
      121 taccactccc tatcagtgat agagaaaagt gaaagtcgag tttaccactc cctatcagtg
      181 atagagaaaa gtgaaagtcg agtttaccac tccctatcag tgatagagaa aagtgaaagt
      241 cgagtttacc actccctatc agtgatagag aaaagtgaaa gtcgagctcg gtacccgggt
      301 cgaggtaggc gtgtacggtg ggaggcctat ataagcagag ctcgtttagt gaaccgtcag
      361 atcgcctgga gacgccatcc acgctgtttt gacctccata gaagacaccg ggaccgatcc
      421 agcctccgcg gccccggcca ccatgaaggt tctgtgggct gcgttgctgg tcacattcct
      481 ggcaggatgc caggccaagg tggagcaagc ggtggagaca gagccggagc ccgagctgcg
      541 ccagcagacc gagtggcaga gcggccagcg ctgggaactg gcactgggtc gcttttggga
      601 ttacctgcgc tgggtgcaga cactgtctga gcaggtgcag gaggagctgc tcagctccca
      661 ggtcacccag gaactgaggg cgctgatgga cgagaccatg aaggagttga aggcctacaa
      721 atcggaactg gaggaacaac tgaccccggt ggcggaggag acgcgggcac ggctgtccaa
      781 ggagctgcag gcggcgcagg cccggctggg cgcggacatg gaggacgtgt gcggccgcct
      841 ggtgcagtac cgcggcgagg tgcaggccat gctcggccag agcaccgagg agctgcgggt
      901 gcgcctcgcc tcccacctgc gcaagctgcg taagcggctc ctccgcgatg ccgatgacct
      961 gcagaagtgc ctggcagtgt accaggccgg ggcccgcgag ggcgccgagc gcggcctcag
     1021 cgccatccgc gagcgcctgg ggcccctggt ggaacagggc cgcgtgcggg ccgccactgt
     1081 gggctccctg gccggccagc cgctacagga gcgggcccag gcctggggcg agcggctgcg
     1141 cgcgcggatg gaggagatgg gcagccggac ccgcgaccgc ctggacgagg tgaaggagca
     1201 ggtggcggag gtgcgcgcca agctggagga gcaggcccag cagatacgcc tgcaggccga
     1261 ggccttccag gcccgcctca agagctggtt cgagcccctg gtggaagaca tgcagcgcca
     1321 gtgggccggg ctggtggaga aggtgcaggc tgccgtgggc accagcgccg cccctgtgcc
     1381 cagcgacaat cacgagggca ggggaagtct tctaacatgc ggggacgtgg aggaaaatcc
     1441 cggcccactc gagatgaccg agtacaagcc cacggtgcgc ctcgccaccc gcgacgacgt
     1501 ccccagggcc gtacgcaccc tcgccgccgc gttcgccgac taccccgcca cgcgccacac
     1561 cgtcgatccg gaccgccaca tcgagcgggt caccgagctg caagaactct tcctcacgcg
     1621 cgtcgggctc gacatcggca aggtgtgggt cgcggacgac ggcgccgcgg tggcggtctg
     1681 gaccacgccg gagagcgtcg aagcgggggc ggtgttcgcc gagatcggcc cgcgcatggc
     1741 cgagttgagc ggttcccggc tggccgcgca gcaacagatg gaaggcctcc tggcgccgca
     1801 ccggcccaag gagcccgcgt ggttcctggc caccgtcggc gtctcgcccg accaccaggg
     1861 caagggtctg ggcagcgccg tcgtgctccc cggagtggag gcggccgagc gcgccggggt
     1921 gcccgccttc ctggagacct ccgcgccccg caacctcccc ttctacgagc ggctcggctt
     1981 caccgtcacc gccgacgtcg aggtgcccga aggaccgcgc acctggtgca tgacccgcaa
     2041 gcccggtgcc tgattaatta agctagctaa ttcgatatca agcttatcga taatcaacct
     2101 ctggattaca aaatttgtga aagattgact ggtattctta actatgttgc tccttttacg
     2161 ctatgtggat acgctgcttt aatgcctttg tatcatgcta ttgcttcccg tatggctttc
     2221 attttctcct ccttgtataa atcctggttg ctgtctcttt atgaggagtt gtggcccgtt
     2281 gtcaggcaac gtggcgtggt gtgcactgtg tttgctgacg caacccccac tggttggggc
     2341 attgccacca cctgtcagct cctttccggg actttcgctt tccccctccc tattgccacg
     2401 gcggaactca tcgccgcctg ccttgcccgc tgctggacag gggctcggct gttgggcact
     2461 gacaattccg tggtgttgtc ggggaaatca tcgtcctttc cttggctgct cgcctgtgtt
     2521 gccacctgga ttctgcgcgg gacgtccttc tgctacgtcc cttcggccct caatccagcg
     2581 gaccttcctt cccgcggcct gctgccggct ctgcggcctc ttccgcgtct tcgccttcgc
     2641 cctcagacga gtcggatctc cctttgggcc gcctccccgc atcgataccg tcgacctcga
     2701 gacctagaaa aacatggagc aatcacaagt agcaatacag cagctaccaa tgctgattgt
     2761 gcctggctag aagcacaaga ggaggaggag gtgggttttc cagtcacacc tcaggtacct
     2821 ttaagaccaa tgacttacaa ggcagctgta gatcttagcc actttttaaa agaaaagggg
     2881 ggactggaag ggctaattca ctcccaacga agacaagata tccttgatct gtggatctac
     2941 cacacacaag gctacttccc tgattggcag aactacacac cagggccagg gatcagatat
     3001 ccactgacct ttggatggtg ctacaagcta gtaccagttg agcaagagaa ggtagaagaa
     3061 gccaatgaag gagagaacac ccgcttgtta caccctgtga gcctgcatgg gatggatgac
     3121 ccggagagag aagtattaga gtggaggttt gacagccgcc tagcatttca tcacatggcc
     3181 cgagagctgc atccggactg tactgggtct ctctggttag accagatctg agcctgggag
     3241 ctctctggct aactagggaa cccactgctt aagcctcaat aaagcttgcc ttgagtgctt
     3301 caagtagtgt gtgcccgtct gttgtgtgac tctggtaact agagatccct cagacccttt
     3361 tagtcagtgt ggaaaatctc tagcagggcc cgtttaaacc cgctgatcag cctcgactgt
     3421 gccttctagt tgccagccat ctgttgtttg cccctccccc gtgccttcct tgaccctgga
     3481 aggtgccact cccactgtcc tttcctaata aaatgaggaa attgcatcgc attgtctgag
     3541 taggtgtcat tctattctgg ggggtggggt ggggcaggac agcaaggggg aggattggga
     3601 agacaatagc aggcatgctg gggatgcggt gggctctatg gcttctgagg cggaaagaac
     3661 cagctggggc tctagggggt atccccacgc gccctgtagc ggcgcattaa gcgcggcggg
     3721 tgtggtggtt acgcgcagcg tgaccgctac acttgccagc gccctagcgc ccgctccttt
     3781 cgctttcttc ccttcctttc tcgccacgtt cgccggcttt ccccgtcaag ctctaaatcg
     3841 ggggctccct ttagggttcc gatttagtgc tttacggcac ctcgacccca aaaaacttga
     3901 ttagggtgat ggttcacgta gtgggccatc gccctgatag acggtttttc gccctttgac
     3961 gttggagtcc acgttcttta atagtggact cttgttccaa actggaacaa cactcaaccc
     4021 tatctcggtc tattcttttg atttataagg gattttgccg atttcggcct attggttaaa
     4081 aaatgagctg atttaacaaa aatttaacgc gaattaattc tgtggaatgt gtgtcagtta
     4141 gggtgtggaa agtccccagg ctccccagca ggcagaagta tgcaaagcat gcatctcaat
     4201 tagtcagcaa ccaggtgtgg aaagtcccca ggctccccag caggcagaag tatgcaaagc
     4261 atgcatctca attagtcagc aaccatagtc ccgcccctaa ctccgcccat cccgccccta
     4321 actccgccca gttccgccca ttctccgccc catggctgac taattttttt tatttatgca
     4381 gaggccgagg ccgcctctgc ctctgagcta ttccagaagt agtgaggagg cttttttgga
     4441 ggcctaggct tttgcaaaaa gctcccggga gcttgtatat ccattttcgg atctgatcag
     4501 cacgtgttga caattaatca tcggcatagt atatcggcat agtataatac gacaaggtga
     4561 ggaactaaac catggccaag ttgaccagtg ccgttccggt gctcaccgcg cgcgacgtcg
     4621 ccggagcggt cgagttctgg accgaccggc tcgggttctc ccgggacttc gtggaggacg
     4681 acttcgccgg tgtggtccgg gacgacgtga ccctgttcat cagcgcggtc caggaccagg
     4741 tggtgccgga caacaccctg gcctgggtgt gggtgcgcgg cctggacgag ctgtacgccg
     4801 agtggtcgga ggtcgtgtcc acgaacttcc gggacgcctc cgggccggcc atgaccgaga
     4861 tcggcgagca gccgtggggg cgggagttcg ccctgcgcga cccggccggc aactgcgtgc
     4921 acttcgtggc cgaggagcag gactgacacg tgctacgaga tttcgattcc accgccgcct
     4981 tctatgaaag gttgggcttc ggaatcgttt tccgggacgc cggctggatg atcctccagc
     5041 gcggggatct catgctggag ttcttcgccc accccaactt gtttattgca gcttataatg
     5101 gttacaaata aagcaatagc atcacaaatt tcacaaataa agcatttttt tcactgcatt
     5161 ctagttgtgg tttgtccaaa ctcatcaatg tatcttatca tgtctgtata ccgtcgacct
     5221 ctagctagag cttggcgtaa tcatggtcat agctgtttcc tgtgtgaaat tgttatccgc
     5281 tcacaattcc acacaacata cgagccggaa gcataaagtg taaagcctgg ggtgcctaat
     5341 gagtgagcta actcacatta attgcgttgc gctcactgcc cgctttccag tcgggaaacc
     5401 tgtcgtgcca gctgcattaa tgaatcggcc aacgcgcggg gagaggcggt ttgcgtattg
     5461 ggcgctcttc cgcttcctcg ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag
     5521 cggtatcagc tcactcaaag gcggtaatac ggttatccac agaatcaggg gataacgcag
     5581 gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc
     5641 tggcgttttt ccataggctc cgcccccctg acgagcatca caaaaatcga cgctcaagtc
     5701 agaggtggcg aaacccgaca ggactataaa gataccaggc gtttccccct ggaagctccc
     5761 tcgtgcgctc tcctgttccg accctgccgc ttaccggata cctgtccgcc tttctccctt
     5821 cgggaagcgt ggcgctttct catagctcac gctgtaggta tctcagttcg gtgtaggtcg
     5881 ttcgctccaa gctgggctgt gtgcacgaac cccccgttca gcccgaccgc tgcgccttat
     5941 ccggtaacta tcgtcttgag tccaacccgg taagacacga cttatcgcca ctggcagcag
     6001 ccactggtaa caggattagc agagcgaggt atgtaggcgg tgctacagag ttcttgaagt
     6061 ggtggcctaa ctacggctac actagaagaa cagtatttgg tatctgcgct ctgctgaagc
     6121 cagttacctt cggaaaaaga gttggtagct cttgatccgg caaacaaacc accgctggta
     6181 gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag
     6241 atcctttgat cttttctacg gggtctgacg ctcagtggaa cgaaaactca cgttaaggga
     6301 ttttggtcat gagattatca aaaaggatct tcacctagat ccttttaaat taaaaatgaa
     6361 gttttaaatc aatctaaagt atatatgagt aaacttggtc tgacagttac caatgcttaa
     6421 tcagtgaggc acctatctca gcgatctgtc tatttcgttc atccatagtt gcctgactcc
     6481 ccgtcgtgta gataactacg atacgggagg gcttaccatc tggccccagt gctgcaatga
     6541 taccgcgaga cccacgctca ccggctccag atttatcagc aataaaccag ccagccggaa
     6601 gggccgagcg cagaagtggt cctgcaactt tatccgcctc catccagtct attaattgtt
     6661 gccgggaagc tagagtaagt agttcgccag ttaatagttt gcgcaacgtt gttgccattg
     6721 ctacaggcat cgtggtgtca cgctcgtcgt ttggtatggc ttcattcagc tccggttccc
     6781 aacgatcaag gcgagttaca tgatccccca tgttgtgcaa aaaagcggtt agctccttcg
     6841 gtcctccgat cgttgtcaga agtaagttgg ccgcagtgtt atcactcatg gttatggcag
     6901 cactgcataa ttctcttact gtcatgccat ccgtaagatg cttttctgtg actggtgagt
     6961 actcaaccaa gtcattctga gaatagtgta tgcggcgacc gagttgctct tgcccggcgt
     7021 caatacggga taataccgcg ccacatagca gaactttaaa agtgctcatc attggaaaac
     7081 gttcttcggg gcgaaaactc tcaaggatct taccgctgtt gagatccagt tcgatgtaac
     7141 ccactcgtgc acccaactga tcttcagcat cttttacttt caccagcgtt tctgggtgag
     7201 caaaaacagg aaggcaaaat gccgcaaaaa agggaataag ggcgacacgg aaatgttgaa
     7261 tactcatact cttccttttt caatattatt gaagcattta tcagggttat tgtctcatga
     7321 gcggatacat atttgaatgt atttagaaaa ataaacaaat aggggttccg cgcacatttc
     7381 cccgaaaagt gccacctgac gtcgacggat cgggagatct cccgatcccc tatggtgcac
     7441 tctcagtaca atctgctctg atgccgcata gttaagccag tatctgctcc ctgcttgtgt
     7501 gttggaggtc gctgagtagt gcgcgagcaa aatttaagct acaacaaggc aaggcttgac
     7561 cgacaattgc atgaagaatc tgcttagggt taggcgtttt gcgctgcttc gcgatgtacg
     7621 ggccagatat acgcgttgac attgattatt gactagttat taatagtaat caattacggg
     7681 gtcattagtt catagcccat atatggagtt ccgcgttaca taacttacgg taaatggccc
     7741 gcctggctga ccgcccaacg acccccgccc attgacgtca ataatgacgt atgttcccat
     7801 agtaacgcca atagggactt tccattgacg tcaatgggtg gagtatttac ggtaaactgc
     7861 ccacttggca gtacatcaag tgtatcatat gccaagtacg ccccctattg acgtcaatga
     7921 cggtaaatgg cccgcctggc attatgccca gtacatgacc ttatgggact ttcctacttg
     7981 gcagtacatc tacgtattag tcatcgctat taccatggtg atgcggtttt ggcagtacat
     8041 caatgggcgt ggatagcggt ttgactcacg gggatttcca agtctccacc ccattgacgt
     8101 caatgggagt ttgttttggc accaaaatca acgggacttt ccaaaatgtc gtaacaactc
     8161 cgccccattg acgcaaatgg gcggtaggcg tgtacggtgg gaggtctata taagcagcgc
     8221 gttttgcctg tactgggtct ctctggttag accagatctg agcctgggag ctctctggct
     8281 aactagggaa cccactgctt aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt
     8341 gtgcccgtct gttgtgtgac tctggtaact agagatccct cagacccttt tagtcagtgt
     8401 ggaaaatctc tagcagtggc gcccgaacag ggacttgaaa gcgaaaggga aaccagagga
     8461 gctctctcga cgcaggactc ggcttgctga agcgcgcacg gcaagaggcg aggggcggcg
     8521 actggtgagt acgccaaaaa ttttgactag cggaggctag aaggagagag atgggtgcga
     8581 gagcgtcagt attaagcggg ggagaattag atcgcgatgg gaaaaaattc ggttaaggcc
     8641 agggggaaag aaaaaatata aattaaaaca tatagtatgg gcaagcaggg agctagaacg
     8701 attcgcagtt aatcctggcc tgttagaaac atcagaaggc tgtagacaaa tactgggaca
     8761 gctacaacca tcccttcaga caggatcaga agaacttaga tcattatata atacagtagc
     8821 aaccctctat tgtgtgcatc aaaggataga gataaaagac accaaggaag ctttagacaa
     8881 gatagaggaa gagcaaaaca aaagtaagac caccgcacag caagcggccg ctgatcttca
     8941 gacctggagg aggagatatg agggacaatt ggagaagtga attatataaa tataaagtag
     9001 taaaaattga accattagga gtagcaccca ccaaggcaaa gagaagagtg gtgcagagag
     9061 aaaaaagagc agtgggaata ggagctttgt tccttgggtt cttgggagca gcaggaagca
     9121 ctatgggcgc agcgtcaatg acgctgacgg tacaggccag acaattattg tctggtatag
     9181 tgcagcagca gaacaatttg ctgagggcta ttgaggcgca acagcatctg ttgcaactca
     9241 cagtctgggg catcaagcag ctccaggcaa gaatcctggc tgtggaaaga tacctaaagg
     9301 atcaacagct cctggggatt tggggttgct ctggaaaact catttgcacc actgctgtgc
     9361 cttggaatgc tagttggagt aataaatctc tggaacagat ttggaatcac acgacctgga
     9421 tggagtggga cagagaaatt aacaattaca caagcttaat acactcctta attgaagaat
     9481 cgcaaaacca gcaagaaaag aatgaacaag aattattgga attagataaa tgggcaagtt
     9541 tgtggaattg gtttaacata acaaattggc tgtggtatat aaaattattc ataatgatag
     9601 taggaggctt ggtaggttta agaatagttt ttgctgtact ttctatagtg aatagagtta
     9661 ggcagggata ttcaccatta tcgtttcaga cccacctccc aaccccgagg ggacccgaca
     9721 ggcccgaagg aatagaagaa gaaggtggag agagagacag agacagatcc attcgattag
     9781 tgaacggatc ggcactgcgt gcgccaattc tgcagacaaa tggcagtatt catccacaat
     9841 tttaaaagaa aaggggggat tggggggtac agtgcagggg aaagaatagt agacataata
     9901 gcaacagaca tacaaactaa agaattacaa aaacaaatta caaaaattca aaattttcgg
     9961 gtttattaca gggacagcag agatccagtt tggttagatc tcgagtttac cac
//
