LOCUS       pAAA059                10680 bp ds-DNA     circular     13-JUN-2022
DEFINITION  .
SOURCE      synthetic DNA construct                                            
  ORGANISM  synthetic DNA construct                                            
FEATURES             Location/Qualifiers
     CDS                complement(153..1595)
                        /label="pSG5"
                        /ApEinfo_revcolor=#ffef86
                        /ApEinfo_fwdcolor=#ffef86
     ori                1674..2064
                        /label="pSG5 double-stranded origin"
                        /ApEinfo_revcolor=#84b0dc
                        /ApEinfo_fwdcolor=#84b0dc
     origin of transfer complement(2762..2871)
                        /label="RP4 oriT"
                        /ApEinfo_revcolor=#ff9ccd
                        /ApEinfo_fwdcolor=#ff9ccd
     misc_feature       2817..2835
                        /label="Sequencing primer 1 (Fwd)"
                        /ApEinfo_revcolor=#f60021
                        /ApEinfo_fwdcolor=#f60021
     misc_feature       3093..3118
                        /label="Spacer"
                        /ApEinfo_revcolor=#85dae9
                        /ApEinfo_fwdcolor=#85dae9
     Promoter           3119..3181
                        /label="SP30"
                        /ApEinfo_revcolor=#ffef86
                        /ApEinfo_fwdcolor=#ffef86
     Insulator          3182..3256
                        /label="RiboJ"
                        /ApEinfo_revcolor=#ff9ccd
                        /ApEinfo_fwdcolor=#ff9ccd
     RBS                3257..3276
                        /label="R09"
                        /ApEinfo_revcolor=#faac61
                        /ApEinfo_fwdcolor=#faac61
     CDS                3277..7383
                        /label="dCas9"
                        /ApEinfo_revcolor=#ffef86
                        /ApEinfo_fwdcolor=#ffef86
     misc_feature       3282..3309
                        /label="R2"
                        /ApEinfo_revcolor=#0000f9
                        /ApEinfo_fwdcolor=#0000f9
     misc_feature       3289..3311
                        /label="F1"
                        /ApEinfo_revcolor=#b1ff67
                        /ApEinfo_fwdcolor=#b1ff67
     misc_feature       3304..3306
                        /label="mutation_1_D10A"
                        /ApEinfo_revcolor=#e100fa
                        /ApEinfo_fwdcolor=#e100fa
     misc_feature       3580..3598
                        /label="Sequencing primer 2"
                        /ApEinfo_revcolor=#f6001b
                        /ApEinfo_fwdcolor=#f6001b
     misc_feature       4461..4478
                        /label="Sequencing primer 3"
                        /ApEinfo_revcolor=#f60014
                        /ApEinfo_fwdcolor=#f60014
     misc_feature       5341..5359
                        /label="Sequencing primer 4"
                        /ApEinfo_revcolor=#f60017
                        /ApEinfo_fwdcolor=#f60017
     misc_feature       5788..5807
                        /label="F2"
                        /ApEinfo_revcolor=#1900f9
                        /ApEinfo_fwdcolor=#1900f9
     misc_feature       5788..5808
                        /label="R1"
                        /ApEinfo_revcolor=#b1ff67
                        /ApEinfo_fwdcolor=#b1ff67
     misc_feature       5794..5796
                        /label="mutation2_H840A"
                        /ApEinfo_revcolor=#85dae9
                        /ApEinfo_fwdcolor=#85dae9
     misc_feature       6237..6254
                        /label="Sequencing primer 5"
                        /ApEinfo_revcolor=#f60014
                        /ApEinfo_fwdcolor=#f60014
     misc_feature       7101..7119
                        /label="Sequencing primer 6"
                        /ApEinfo_revcolor=#f6001d
                        /ApEinfo_fwdcolor=#f6001d
     misc_feature       7542..7582
                        /label="Fd_Terminator"
                        /ApEinfo_revcolor=#ff0000
                        /ApEinfo_fwdcolor=#ff0000
     misc_feature       7583..7608
                        /label="Spacer"
                        /ApEinfo_revcolor=#85dae9
                        /ApEinfo_fwdcolor=#85dae9
     Promoter           7609..7671
                        /label="SP43"
                        /ApEinfo_revcolor=#ffef86
                        /ApEinfo_fwdcolor=#ffef86
     misc_feature       7672..8128
                        /label="Insert (removed with Bbs1)"
                        /ApEinfo_revcolor=#85dae9
                        /ApEinfo_fwdcolor=#85dae9
     CDS                complement(7680..7976)
                        /label="lacZ-alpha"
                        /ApEinfo_revcolor=#ffef86
                        /ApEinfo_fwdcolor=#ffef86
     promoter           7867..7885
                        /label="T7 promoter"
                        /ApEinfo_revcolor=#346ee0
                        /ApEinfo_fwdcolor=#346ee0
     promoter           complement(7933..7951)
                        /label="T3 promoter"
                        /ApEinfo_revcolor=#346ee0
                        /ApEinfo_fwdcolor=#346ee0
     protein_bind       7996..8012
                        /label="lac operator"
                        /ApEinfo_revcolor=#ff9ccd
                        /ApEinfo_fwdcolor=#ff9ccd
     promoter           complement(8020..8050)
                        /label="lac promoter"
                        /ApEinfo_revcolor=#346ee0
                        /ApEinfo_fwdcolor=#346ee0
     protein_bind       8065..8086
                        /label="CAP binding site"
                        /ApEinfo_revcolor=#ff9ccd
                        /ApEinfo_fwdcolor=#ff9ccd
     misc_RNA           8129..8204
                        /label="gRNA scaffold"
                        /ApEinfo_revcolor=#ff9ccd
                        /ApEinfo_fwdcolor=#ff9ccd
     terminator         8226..8260
                        /label="lambda t0 terminator"
                        /ApEinfo_revcolor=#9d1b1c
                        /ApEinfo_fwdcolor=#9d1b1c
     ori                complement(8302..8890)
                        /label="pUC"
                        /ApEinfo_revcolor=#84b0dc
                        /ApEinfo_fwdcolor=#84b0dc
     CDS                9094..9894
                        /label="ApmR"
                        /ApEinfo_revcolor=#ffef86
                        /ApEinfo_fwdcolor=#ffef86
     misc_feature       10160..10183
                        /label="F3"
                        /ApEinfo_revcolor=#1c1de0
                        /ApEinfo_fwdcolor=#1c1de0
     misc_feature       10162..10184
                        /label="R3"
                        /ApEinfo_revcolor=#b1ff67
                        /ApEinfo_fwdcolor=#b1ff67
ORIGIN
        1 cggacgctcg gactgggcgc gggattccag cagtaaccca ggtccgccgg ccacctcacg
       61 gcaggcagac cctcggcttt cgcgccggga ccgccatgag accgccaccc ggatgtccgg
      121 ggtggcggtc tcatggcggt ccctcagcgg ccctacgcga ccgctgtgtc gacgcggagg
      181 cagtctccgg ggtgctggcc ccagggctgg agtaccgggg cgagcttgcc cttgcagcgg
      241 cggcacacgg gcgacgcggc gccgggcggg ggagtcttga cgtcgacggt gaccggcttc
      301 ggcttcaggc gcttccgaag gctgatcgtc gggcggatcg cctccttctt cggctgacgc
      361 actcggtcga gcgaagcggc cagctcctcc gtgcgggcct cgttggcctt ccggcgcgcc
      421 tcgcggaaag cagcttcctc ctcggacatg acctcgaacc tcatctggtc agcgtcaagg
      481 tcgcccggcg cggccggcgc ttccggggcg ggcgggtcca ggacgtcctt gccccacacc
      541 aggccccagg actcgacgag ccgccggacg cccggtaggc cgtacgtctc ggcgaccttg
      601 atgaggtcga ggcgacgtcc ggcgacgcgg gcgatgtatc ggtaccagat gtaggccggg
      661 atgaccgcga tggcgaccag gccctcggtg tcgtcggtga tctcctcctc ggtgcggacg
      721 tcctgctgga tgccgagttc cttgatcagc cggttcaggt tctgcgaccg gtagtgcttg
      781 cggacctgga acacgccgaa ctcgcgctcg cggtacttct cgacgaacgg gccgggccga
      841 cgaagccgct gcagctcggc ggccgccgcg tcgcccaggt cgagcggtcc catgcggtcg
      901 tcgccgcgac cggccttgaa gttctgtccg gccagctcca ggccgatctt ggcgacgccg
      961 cccttggtct tgtcgccgtc cttgtagagg tagcgggcct gcttgcccgc atcgccgtca
     1021 gcggcgtccg cgccgttgag tgggcgcacg tcggtgccgt ggcccttgcc ctcacaggag
     1081 caaccgggcc ggtcgcacgt ctcgctgacg gtgtagccgc ccgcggattc gaccccggcg
     1141 gcccaggctc cggcgagtgc gtcgcggaac gcggcctggg cgtccgggcc gagcacctcg
     1201 cgggtgaccc agagcgtgtg ccagtgcagg tgccagccgg agccccagcc gaaggtgtcc
     1261 tcgaaggccc gctcgtagcc gatgatcccg aagtcgtcgc gcatcgtgcg ccagcggcgg
     1321 ccggacgagc cgtacgcgcc cttccagccg tcgtgcaaga ccgcgaccag gccgtgccgc
     1381 attcccttgc ggacggtgcc gaacgccatg cgctcgaagt ggcgcaacgt gttcgtgcca
     1441 aggtgcagcc cgtacccggc gtccgcgaga ccgtcggcgg cgagctgcac gttcgagccc
     1501 cgtacggcca ggatgcggct catgcaccac gggcaggtgt ggacgttgtt gcagcggcac
     1561 gtgttgcccc acgtcgcctc gcccggcttc cacatcagct cggccgtccc ggcagtgagc
     1621 cgggtcccgc agcccttgaa cgcctcgttc agcgacaccg tctggtgccg gtcccgccgg
     1681 gcgaaccgct cgtcgcgcgg gtcctcccgc ccggctgtcg cggcaccctc gtttggggta
     1741 gaacccgttc cagttacagc gctctgacct gcagtggacg gagattttcc cttactacta
     1801 aagcccgcgt ccggattacc cgctgtagtc gtgcttgcta cgctgcgtga ctggtccgca
     1861 atgagacgct ttgcgcgctt tcggcaggcg tccgagcagt agattttggg gcgcttcccg
     1921 gggatgtgga cgatcggggt gccgcagtgg cacttcggtc cggcggggcg cggtggtgtc
     1981 gacgcgctgt tctctcgtac gctcgtcaca gagcaaacgt cctcactcgg catgctgcgc
     2041 cggttcgggg gcggcgagcc cgggaggcca atcccgggct cgtgccattt ctgggtcctg
     2101 ttgatcctgg cattggtgtg gccgttcatt gcccctgctc gctcctgacg cgccgataga
     2161 cgtccgatac gcccggtgct ggtgggattt gataggtcgg aagaagcccc gcccgggcct
     2221 gggcggggct tcctgtgcgt caggacctcc tcgtcgtgag cctcttcggc ctatggacgg
     2281 agtgacctcg tgatccgtta cagccgcgcg cgctcgcgta gagcggtctc atcagttcca
     2341 cgaacggtcc tcttcgcaga tcagggcgtt ggggcggagt ctcaccaagg actacgtctg
     2401 ctggcgattt ccgttacacc ccgggcggtg gccggcgcac acgcgcgccc gcgttgggca
     2461 gtgcagaaag tgcagaaacc taggcgctga tggtccaggt ccacggttcg tcgtcggcgg
     2521 cggcgcgggc ggcggcgtcg gccagggcgc gggcgagacc ggctacggcg ggcttgatgc
     2581 gccggttgcg ggcgaccttg agcagctagt atgcaggtcg acggatcttt tccgctgcat
     2641 aaccctgctt cggggtcatt atagcgattt tttcggtata tccatccttt ttcgcacgat
     2701 atacaggatt ttgccaaagg gttcgtgtag actttccttg gtgtatccaa cggcgtcagc
     2761 cgggcaggat aggtgaagta ggcccacccg cgagcgggtg ttccttcttc actgtccctt
     2821 attcgcacct ggcggtgctc aacgggaatc ctgctctgcg aggctggccg gctaccgccg
     2881 gcgtaacaga tgagggcaag cggatggctg atgaaaccaa gccaaccagg aagggcagcc
     2941 cacctatcaa ggtgtactgc cttccagacg aacgaagagc gattgaggaa aaggcggcgg
     3001 cggccggcat gagcctgtcg gcctacctgc tggccgtcgg ccagggctac aaaatcacgg
     3061 gcgtcgtgga ctatgagcac gtccgcgagc tgGACAGCGT GCAGGACTGG GGGAGTTAtg
     3121 ttcacattcg aaccgtctct gctTTGACAt cgtgtggcgc ttgggtgTAA AGTcgtggcc
     3181 Attaaacaaa attatttgta gaggctgttt cgtcctcacg gactcatcag accggaaagc
     3241 acatccggtg acagctaact acgaagggga gtcagtatgg acaagaagta cagcatcggc
     3301 ctggccatcg gcaccaacag cgtgggctgg gcggtcatca ccgacgagta caaggtcccc
     3361 tccaagaagt tcaaggtcct gggcaacacc gaccggcact cgatcaagaa gaacctgatc
     3421 ggcgccctgc tcttcgacag cggcgaaacc gccgaggcga cccgcctgaa gcggaccgcc
     3481 cgtcgccgct acacccggcg caagaaccgc atctgctacc tgcaggagat cttctccaac
     3541 gagatggcca aggtcgacga ctcgttcttc caccggctcg aggagagctt cctggtggag
     3601 gaggacaaga agcacgagcg ccacccgatc ttcggcaaca tcgtcgacga ggtggcctac
     3661 cacgagaagt accccaccat ctaccacctc cgcaagaagc tggtcgactc gaccgacaag
     3721 gcggacctgc ggctcatcta cctggccctc gcgcacatga tcaagttccg cggccacttc
     3781 ctcatcgagg gcgacctgaa cccggacaac tccgacgtcg acaagctctt catccagctg
     3841 gtgcagacct acaaccagct gttcgaggag aaccccatca acgccagcgg cgtcgacgcc
     3901 aaggcgatcc tctccgcgcg cctgagcaag tcccggcgcc tggagaacct catcgcccag
     3961 ctgccgggcg agaagaagaa cggcctcttc ggcaacctga tcgcgctgtc gctcggcctg
     4021 acccccaact tcaagagcaa cttcgacctg gccgaggacg cgaagctcca gctgtccaag
     4081 gacacctacg acgacgacct ggacaacctg ctcgcccaga tcggcgacca gtacgcggac
     4141 ctcttcctgg ccgcgaagaa cctctcggac gccatcctgc tcagcgacat cctgcgggtc
     4201 aacaccgaga tcaccaaggc cccgctgtcg gcgagcatga tcaagcggta cgacgagcac
     4261 caccaggacc tgaccctgct caaggccctc gtgcgccagc agctgcccga gaagtacaag
     4321 gagatcttct tcgaccagtc caagaacggc tacgccggct acatcgacgg cggcgcgtcg
     4381 caggaggagt tctacaagtt catcaagccg atcctggaga agatggacgg caccgaggag
     4441 ctgctcgtca agctgaaccg cgaggacctg ctccgcaagc agcggacctt cgacaacggc
     4501 tccatcccgc accagatcca cctgggcgag ctccacgcca tcctccggcg ccaggaggac
     4561 ttctacccct tcctgaagga caaccgcgag aagatcgaga agatcctgac cttccggatc
     4621 ccgtactacg tcggccccct ggcccgcggc aactcccggt tcgcgtggat gacccggaag
     4681 tcggaggaaa ccatcacccc gtggaacttc gaggaggtcg tggacaaggg cgcctccgcg
     4741 cagtcgttca tcgagcgcat gaccaacttc gacaagaacc tcccgaacga gaaggtcctg
     4801 cccaagcaca gcctgctcta cgagtacttc accgtgtaca acgagctgac caaggtcaag
     4861 tacgtgaccg agggcatgcg gaagccggcc ttcctgtccg gcgagcagaa gaaggcgatc
     4921 gtcgacctgc tcttcaagac caaccgcaag gtcaccgtga agcagctgaa ggaggactac
     4981 ttcaagaaga tcgagtgctt cgactccgtc gagatctcgg gcgtggagga ccgcttcaac
     5041 gcctccctgg gcacctacca cgacctgctc aagatcatca aggacaagga cttcctcgac
     5101 aacgaggaga acgaggacat cctggaggac atcgtcctca ccctgaccct cttcgaggac
     5161 cgcgagatga tcgaggagcg gctcaagacc tacgcccacc tgttcgacga caaggtgatg
     5221 aagcagctga agcggcgccg gtacaccggc tggggccgcc tctcccggaa gctgatcaac
     5281 ggcatccggg acaagcagag cggcaagacc atcctggact tcctcaagtc cgacggcttc
     5341 gccaaccgca acttcatgca gctcatccac gacgactcgc tgaccttcaa ggaggacatc
     5401 cagaaggccc aggtgtccgg ccagggcgac agcctccacg agcacatcgc caacctggcg
     5461 ggctccccgg cgatcaagaa gggcatcctc cagaccgtca aggtcgtgga cgagctggtc
     5521 aaggtgatgg gccgccacaa gcccgagaac atcgtgatcg agatggcccg ggagaaccag
     5581 accacccaga agggccagaa gaactcccgc gagcggatga agcgcatcga ggagggcatc
     5641 aaggagctcg gctcgcagat cctgaaggag cacccggtcg agaacaccca gctccagaac
     5701 gagaagctgt acctctacta cctgcagaac ggccgcgaca tgtacgtgga ccaggagctc
     5761 gacatcaacc ggctgagcga ctacgacgtc gacgccatcg tgccgcagtc cttcctgaag
     5821 gacgactcga tcgacaacaa ggtcctgacc cgctccgaca agaaccgggg caagtccgac
     5881 aacgtgccct cggaggaggt cgtgaagaag atgaagaact actggcgcca gctgctcaac
     5941 gccaagctca tcacccagcg caagttcgac aacctgacca aggccgagcg gggcggcctg
     6001 tcggagctcg acaaggcggg cttcatcaag cgccagctcg tcgaaacccg gcagatcacc
     6061 aagcacgtgg cccagatcct ggacagccgg atgaacacca agtacgacga gaacgacaag
     6121 ctgatccgcg aggtcaaggt gatcaccctc aagagcaagc tggtgtccga cttccgcaag
     6181 gacttccagt tctacaaggt ccgggagatc aacaactacc accacgccca cgacgcgtac
     6241 ctgaacgccg tcgtgggcac cgcgctgatc aagaagtacc cgaagctgga gtccgagttc
     6301 gtctacggcg actacaaggt ctacgacgtg cgcaagatga tcgccaagtc ggagcaggag
     6361 atcggcaagg ccaccgcgaa gtacttcttc tacagcaaca tcatgaactt cttcaagacc
     6421 gagatcaccc tggccaacgg cgagatccgc aagcggcccc tgatcgaaac caacggcgaa
     6481 accggcgaga tcgtctggga caagggccgc gacttcgcca ccgtccggaa ggtgctgtcc
     6541 atgccgcagg tcaacatcgt caagaaaacc gaggtgcaga ccggcggctt cagcaaggag
     6601 tccatcctcc ccaagcgcaa ctcggacaag ctgatcgccc ggaagaagga ctgggacccg
     6661 aagaagtacg gcggcttcga cagccccacc gtcgcctact ccgtgctggt cgtggcgaag
     6721 gtcgagaagg gcaagagcaa gaagctgaag tccgtgaagg agctgctcgg catcaccatc
     6781 atggagcgct cctcgttcga gaagaacccg atcgacttcc tggaggccaa gggctacaag
     6841 gaggtcaaga aggacctcat catcaagctg cccaagtact cgctgttcga gctcgagaac
     6901 ggccgcaagc ggatgctcgc cagcgcgggc gagctgcaga agggcaacga gctggccctc
     6961 ccgtccaagt acgtcaactt cctgtacctc gcgtcccact acgagaagct gaagggctcg
     7021 cccgaggaca acgagcagaa gcagctcttc gtggagcagc acaagcacta cctggacgag
     7081 atcatcgagc agatctcgga gttcagcaag cgggtcatcc tggccgacgc gaacctcgac
     7141 aaggtgctgt ccgcctacaa caagcaccgc gacaagccga tccgggagca ggcggagaac
     7201 atcatccacc tgttcaccct caccaacctg ggcgcccccg ccgcgttcaa gtacttcgac
     7261 accaccatcg accgcaagcg gtacaccagc accaaggagg tcctcgacgc gaccctgatc
     7321 caccagtcca tcaccggcct gtacgaaacc cgcatcgacc tctcccagct cggcggcgac
     7381 tgagaattca gatctacgcg ttccccgcaa aagcggcctt tgactccctg caagcctcag
     7441 cgaccgaata tatcggttat gcgtgggcga tggttgttgt cattgtcggc gcaactatcg
     7501 gtatcaagct gtttaagaaa ttcacctcga aagcaagctg ataaaccgat acaattaaag
     7561 gctccttttg gagccttttt ttGACAGCGT GCAGGACTGG GGGAGTTAtg ttcacattcg
     7621 aaccgtctct gctTTGACAc ggacaagcgc tatggtgTAA AGTcgtggcc Aaagtcttct
     7681 cagccgctac agggcgcgtc ccattcgcca ttcaggctgc gcaactgttg ggaagggcga
     7741 tcggtgcggg cctcttcgct attacgccag ctggcgaaag ggggatgtgc tgcaaggcga
     7801 ttaagttggg taacgccagg gttttcccag tcacgacgtt gtaaaacgac ggccagtgag
     7861 cgcgcgtaat acgactcact atagggcgaa ttgggtaccg ggccccccct cgaggtcctc
     7921 cagcttttgt tccctttagt gagggttaat tgcgcgcttg gcgtaatcat ggtcatagct
     7981 gtttcctgtg tgaaattgtt atccgctcac aattccacac aacatacgag ccggaagcat
     8041 aaagtgtaaa gcctggggtg cctaatgagt gagctaactc acattaattg cgttgcgctc
     8101 actgcccgct ttccaccggt gaagacatgt tttagagcta gaaatagcaa gttaaaataa
     8161 ggctagtccg ttatcaactt gaaaaagtgg caccgagtcg gtgcttttta ctccatctgg
     8221 atttgttcag aacgctcggt tgccgccggg cgttttttat ctagaggcca ggaaccgtaa
     8281 aaaggccgcg ttgctggcgt ttttccatag gctccgcccc cctgacgagc atcacaaaaa
     8341 tcgacgctca agtcagaggt ggcgaaaccc gacaggacta taaagatacc aggcgtttcc
     8401 ccctggaagc tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg gatacctgtc
     8461 cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc tcacgctgta ggtatctcag
     8521 ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac gacccccccg ttcagcccga
     8581 ccgctgcgcc ttatccggta actatcgtct tgagtccaac ccggtaagac acgacttatc
     8641 gccactggca gcagccactg gtaacaggat tagcagagcg aggtatgtag gcggtgctac
     8701 agagttcttg aagtggtggc ctaactacgg ctacactaga agaacagtat ttggtatctg
     8761 cgctctgctg aagccagtta ccttcggaaa aagagttggt agctcttgat ccggcaaaca
     8821 aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa
     8881 aggatctcaa gaagatcctt tgatcttttc tacggggtct gacgctcagt ggaacgaaaa
     8941 ctcacgttaa gggattttgg tcatgagatt atcaaaaagg atcttcacct agatcctttt
     9001 ggttcatgtg cagctccatc agcaaaaggg gatgataagt ttatcaccac cgactatttg
     9061 caacagtgcc gttgatcgtg ctatgatcga ctgatgtcat cagcggtgga gtgcaatgtc
     9121 gtgcaatacg aatggcgaaa agccgagctc atcggtcagc ttctcaacct tggggttacc
     9181 cccggcggtg tgctgctggt ccacagctcc ttccgtagcg tccggcccct cgaagatggg
     9241 ccacttggac tgatcgaggc cctgcgtgct gcgctgggtc cgggagggac gctcgtcatg
     9301 ccctcgtggt caggtctgga cgacgagccg ttcgatcctg ccacgtcgcc cgttacaccg
     9361 gaccttggag ttgtctctga cacattctgg cgcctgccaa atgtaaagcg cagcgcccat
     9421 ccatttgcct ttgcggcagc ggggccacag gcagagcaga tcatctctga tccattgccc
     9481 ctgccacctc actcgcctgc aagcccggtc gcccgtgtcc atgaactcga tgggcaggta
     9541 cttctcctcg gcgtgggaca cgatgccaac acgacgctgc atcttgccga gttgatggca
     9601 aaggttccct atggggtgcc gagacactgc accattcttc aggatggcaa gttggtacgc
     9661 gtcgattatc tcgagaatga ccactgctgt gagcgctttg ccttggcgga caggtggctc
     9721 aaggagaaga gccttcagaa ggaaggtcca gtcggtcatg cctttgctcg gttgatccgc
     9781 tcccgcgaca ttgtggcgac agccctgggt caactgggcc gagatccgtt gatcttcctg
     9841 catccgccag aggcgggatg cgaagaatgc gatgccgctc gccagtcgat tggctgagct
     9901 catgagcgga gaacgagatg acgttggagg ggcaaggtcg cgctgattgc tggggcaaca
     9961 cgtggagcgg atcggggatt gtctttcttc agctcgctga tgatatgctg acgctcaatg
    10021 ccgtttggcc tccgactaac gaaaatcccg catttggacg gctgatccga ttggcacggc
    10081 ggacggcgaa tggcggagca gacgctcgtc cgggggcaat gagatatgaa aaagcctgaa
    10141 ctcaccgcga cgtatcgatg tcgaggttcc tcaggggagc caccccagag aagccctcgg
    10201 agctgagcgg agctatttcc aaagccatgc cagctagaga cagtgcacac cgccaagcat
    10261 ctgcaaaacc ctcgcctgga gggaaagtgc aatgtacgta cgctggtttc cctccagaaa
    10321 gggattctgc ggcttctcaa ctttggaaga agaggcggaa cgaactgctg tttcagccta
    10381 ctcatgtgag gaggctggtc ctttaccctg atgcccgagg gcatcagggt aaaggaccag
    10441 cctcgccaac taggagcgct accaaggcga acacaaccca ggacttggta gaacccttcg
    10501 ccggagaagt tcagactcat cggcatgagc acccctcgcc gcgcactcgg caccggtccg
    10561 tcgaccacca ccaggacgtc gttgtcgacg tcggccccgc ggctcctgcc cgccgaacgc
    10621 gtcgtcgtcg acggcctggt gctcatcgac gagcacccgg agccaggtga aaagcgccgg
//