LOCUS       Exported                7691 bp DNA     circular SYN 20-AUG-2022
DEFINITION  .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7691)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Aug 20, 2022 from SnapGene Viewer 6.0.2
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..7691
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     1..20
                     /label=Forward primer (1-20 bp_20 bp)
                     /note="color: #ff0000; direction: RIGHT"
     CDS             6..944
                     /label=HALOTag
                     /note="color: #ff00ff; direction: RIGHT"
     misc_feature    1201..2004
                     /label=URA3
                     /note="color: #00ffff"
     primer_bind     complement(2233..2252)
                     /label=Reverse primer (2233-2252 bp_20 bp)
                     /note="color: #ff0000; direction: LEFT"
     misc_feature    complement(3169..3828)
                     /label=HIS3
                     /note="color: #a6acb3; direction: LEFT"
     misc_feature    4521..5381
                     /label=AmpR
                     /note="color: #a6acb3"
     misc_feature    6630..6987
                     /label=pCAP2
                     /note="color: #a6acb3"
     misc_feature    join(7020..7691,1..2)
                     /label=ACE1
                     /note="color: #a6acb3"
ORIGIN
        1 caagcggccg ccgctgctgc cgctgcagct gctgcagctg cgatgggttc cgaaatcggt
       61 actggctttc cattcgaccc ccattatgtg gaagtcctgg gcgagcgcat gcactacgtc
      121 gatgttggtc cgcgcgatgg cacccctgtg ctgttcctgc acggtaaccc gacctcctcc
      181 tacgtgtggc gcaacatcat cccgcatgtt gcaccgaccc atcgctgcat tgctccagac
      241 ctgatcggta tgggcaaatc cgacaaacca gacctgggtt atttcttcga cgaccacgtc
      301 cgcttcatgg atgccttcat cgaagccctg ggtctggaag aggtcgtcct ggtcattcac
      361 gactggggct ccgctctggg tttccactgg gccaagcgca atccagagcg cgtcaaaggt
      421 attgcattta tggagttcat ccgccctatc ccgacctggg acgaatggcc agaatttgcc
      481 cgcgagacct tccaggcctt ccgcaccacc gacgtcggcc gcaagctgat catcgatcag
      541 aacgttttta tcgagggtac gctgccgatg ggtgtcgtcc gcccgctgac tgaagtcgag
      601 atggaccatt accgcgagcc gttcctgaat cctgttgacc gcgagccact gtggcgcttc
      661 ccaaacgagc tgccaatcgc cggtgagcca gcgaacatcg tcgcgctggt cgaagaatac
      721 atggactggc tgcaccagtc ccctgtcccg aagctgctgt tctggggcac cccaggcgtt
      781 ctgatcccac cggccgaagc cgctcgcctg gccaaaagcc tgcctaactg caaggctgtg
      841 gacatcggcc cgggtctgaa tctgctgcaa gaagacaacc cggacctgat cggcagcgag
      901 atcgcgcgct ggctgtctac tctggagatt tccggtccga ccgcggggtt aatgtggctg
      961 tggtttcagg gtccataaag cttttcaatt catctttttt ttttttgttc ttttttttga
     1021 ttccggtttc tttgaaattt ttttgattcg gtaatctccg agcagaagga agaacgaagg
     1081 aaggagcaca gacttagatt ggtatatata cgcatatgtg gtgttgaaga aacatgaaat
     1141 tgcccagtat tcttaaccca actgcacaga acaaaaacct gcaggaaacg aagataaatc
     1201 atgtcgaaag ctacatataa ggaacgtgct gctactcatc ctagtcctgt tgctgccaag
     1261 ctatttaata tcatgcacga aaagcaaaca aacttgtgtg cttcattgga tgttcgtacc
     1321 accaaggaat tactggagtt agttgaagca ttaggtccca aaatttgttt actaaaaaca
     1381 catgtggata tcttgactga tttttccatg gagggcacag ttaagccgct aaaggcatta
     1441 tccgccaagt acaatttttt actcttcgaa gacagaaaat ttgctgacat tggtaataca
     1501 gtcaaattgc agtactctgc gggtgtatac agaatagcag aatgggcaga cattacgaat
     1561 gcacacggtg tggtgggccc aggtattgtt agcggtttga agcaggcggc ggaagaagta
     1621 acaaaggaac ctagaggcct tttgatgtta gcagaattgt catgcaaggg ctccctagct
     1681 actggagaat atactaaggg tactgttgac attgcgaaga gcgacaaaga ttttgttatc
     1741 ggctttattg ctcaaagaga catgggtgga agagatgaag gttacgattg gttgattatg
     1801 acacccggtg tgggtttaga tgacaaggga gacgcattgg gtcaacagta tagaaccgtg
     1861 gatgatgtgg tctctacagg atctgacatt attattgttg gaagaggact atttgcaaag
     1921 ggaagggatg ctaaggtaga gggtgaacgt tacagaaaag caggctggga agcatatttg
     1981 agaagatgcg gccagcaaaa ctaaaaaact gtattataag taaatgcatg tatactaaac
     2041 tcacaaatta gagcttcaat ttaattatat cagttattac ccgggaatct cggtcgtaat
     2101 gatttctata atgacgaaaa aaaaaaaatt ggaaagaaaa agcttcatgg cctttataaa
     2161 aaggaactat ccaatacctc gccagaacca agtaacagta ttttacgggg cacaaatcaa
     2221 gaacaataag acaggactgt aaagatggac gcgagctcca attcgcccta tagtgagtcg
     2281 tattacaatt cactggccgt cgttttacaa cgtcgtgact gggaaaaccc tggcgttacc
     2341 caacttaatc gccttgcagc acatcccccc ttcgccagct ggcgtaatag cgaagaggcc
     2401 cgcaccgatc gcccttccca acagttgcgc agcctgaatg gcgaatggcg cgacgcgccc
     2461 tgtagcggcg cattaagcgc ggcgggtgtg gtggttacgc gcagcgtgac cgctacactt
     2521 gccagcgccc tagcgcccgc tcctttcgct ttcttccctt cctttctcgc cacgttcgcc
     2581 ggctttcccc gtcaagctct aaatcggggg ctccctttag ggttccgatt tagtgcttta
     2641 cggcacctcg accccaaaaa acttgattag ggtgatggtt cacgtagtgg gccatcgccc
     2701 tgatagacgg tttttcgccc tttgacgttg gagtccacgt tctttaatag tggactcttg
     2761 ttccaaactg gaacaacact caaccctatc tcggtctatt cttttgattt ataagggatt
     2821 ttgccgattt cggcctattg gttaaaaaat gagctgattt aacaaaaatt taacgcgaat
     2881 tttaacaaaa tattaacgtt tacaatttcc tgatgcggta ttttctcctt acgcatctgt
     2941 gcggtatttc acaccgcata tgatccgtcg agttcaagag aaaaaaaaag aaaaagcaaa
     3001 aagaaaaaag gaaagcgcgc ctcgttcaga atgacacgta tagaatgatg cattaccttg
     3061 tcatcttcag tatcatactg ttcgtataca tacttactga cattcatagg tatacatata
     3121 tacacatgta tatatatcgt atgctgcagc tttaaataat cggtgtcact acataagaac
     3181 acctttggtg gagggaacat cgttggtacc attgggcgag gtggcttctc ttatggcaac
     3241 cgcaagagcc ttgaacgcac tctcactacg gtgatgatca ttcttgcctc gcagacaatc
     3301 aacgtggagg gtaattctgc tagcctctgc aaagctttca agaaaatgcg ggatcatctc
     3361 gcaagagaga tctcctactt tctccctttg caaaccaagt tcgacaactg cgtacggcct
     3421 gttcgaaaga tctaccaccg ctctggaaag tgcctcatcc aaaggcgcaa atcctgatcc
     3481 aaaccttttt actccacgcg ccagtagggc ctctttaaaa gcttgaccga gagcaatccc
     3541 gcagtcttca gtggtgtgat ggtcgtctat gtgtaagtca ccaatgcact caacgattag
     3601 cgaccagccg gaatgcttgg ccagagcatg tatcatatgg tccagaaacc ctatacctgt
     3661 gtggacgtta atcacttgcg attgtgtggc ctgttctgct actgcttctg cctctttttc
     3721 tgggaagatc gagtgctcta tcgctagggg accacccttt aaagagatcg caatctgaat
     3781 cttggtttca tttgtaatac gctttactag ggctttctgc tctgtcatct ttgccttcgt
     3841 ttatcttgcc tgctcatttt ttagtatatt cttcgaagaa atcacattac tttatataat
     3901 gtataattca ttatgtgata atgccaatcg ctaagaaaaa aaaagagtca tccgctaggt
     3961 ggaaaaaaaa aaatgaaaat cattaccgag gcataaaaaa atatagagtg tactagagga
     4021 ggccaagagt aatagaaaaa gaaaattgcg ggaaaggact gtgttatgac ttccctgact
     4081 aatgccgtgt tcaaacgata cctggcagtg actcctagcg ctcaccaagc tcttaaaacg
     4141 gaattatggt gcactctcag tacaatctgc tctgatgccg catagttaag ccagccccga
     4201 cacccgccaa cacccgctga cgcgccctga cgggcttgtc tgctcccggc atccgcttac
     4261 agacaagctg tgaccgtctc cgggagctgc atgtgtcaga ggttttcacc gtcatcaccg
     4321 aaacgcgcga gacgaaaggg cctcgtgata cgcctatttt tataggttaa tgtcatgata
     4381 ataagacgtc aggtggcact tttcggggaa atgtgcgcgg aacccctatt tgtttatttt
     4441 tctaaataca ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat
     4501 aatattgaaa aaggaagagt atgagtattc aacatttccg tgtcgccctt attccctttt
     4561 ttgcggcatt ttgccttcct gtttttgctc acccagaaac gctggtgaaa gtaaaagatg
     4621 ctgaagatca gttgggtgca cgagtgggtt acatcgaact ggatctcaac agcggtaaga
     4681 tccttgagag ttttcgcccc gaagaacgtt ttccaatgat gagcactttt aaagttctgc
     4741 tatgtggcgc ggtattatcc cgtattgacg ccgggcaaga gcaactcggt cgccgcatac
     4801 actattctca gaatgacttg gttgagtact caccagtcac agaaaagcat cttacggatg
     4861 gcatgacagt aagagaatta tgcagtgctg ccataaccat gagtgataac actgcggcca
     4921 acttacttct gacaacgatc ggaggaccga aggagctaac cgcttttttt cacaacatgg
     4981 gggatcatgt aactcgcctt gatcgttggg aaccggagct gaatgaagcc ataccaaacg
     5041 acgagcgtga caccacgatg cctgtagcaa tggcaacaac gttgcgcaaa ctattaactg
     5101 gcgaactact tactctagct tcccggcaac aattaataga ctggatggag gcggataaag
     5161 ttgcaggacc acttctgcgc tcggcccttc cggctggctg gtttattgct gataaatctg
     5221 gagccggtga gcgtgggtct cgcggtatca ttgcagcact ggggccagat ggtaagccct
     5281 cccgtatcgt agttatctac acgacgggca gtcaggcaac tatggatgaa cgaaatagac
     5341 agatcgctga gataggtgcc tcactgatta agcattggta actgtcagac caagtttact
     5401 catatatact ttagattgat ttaaaacttc atttttaatt taaaaggatc taggtgaaga
     5461 tcctttttga taatctcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt
     5521 cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct
     5581 gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc
     5641 taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgtcc
     5701 ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc
     5761 tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg
     5821 ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt
     5881 cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg
     5941 agcattgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg
     6001 gcagggtcgg aacaggagag cgcacgaggg agcttccagg ggggaacgcc tggtatcttt
     6061 atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag
     6121 gggggccgag cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt
     6181 gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg gataaccgta
     6241 ttaccgcctt tgagtgagct gataccgctc gccgcagccg aacgaccgag cgcagcgagt
     6301 cagtgagcga ggaagcggaa gagcgcccaa tacgcaaacc gcctctcccc gcgcgttggc
     6361 cgattcatta atgcagctgg cacgacaggt ttcccgactg gaaagcgggc agtgagcgca
     6421 acgcaattaa tgtgagttac ctcactcatt aggcacccca ggctttacac tttatgcttc
     6481 cggctcctat gttgtgtgga attgtgagcg gataacaatt tcacacagga aacagctatg
     6541 accatgatta cgccaagctc ggaattaacc ctcactaaag ggaacaaaag ctgggtaccg
     6601 ggccccccct cgaggtcgac ggtatcgatg tagacgtatt aaagctcaat gatcctacaa
     6661 gacattaagt aaaaagggga agcgatctta aatccacatg aatttccttt ctctttttct
     6721 tcttcttctg ttcccttttt ctttcttctt ccttcaacgt ctacgtaaat ataatgtata
     6781 acatctccac tttcctttcc cttatagcgt tatgttacct gtttaaactc taaattcttc
     6841 atctactact gctgttacta catgtatata tgttcatttt tttttttttt tttcaccacg
     6901 gaaaacgtaa aaatgagagc atttgtaaca aataaaggag aactgttcaa tcaagtgaca
     6961 tacaaaaact ctctccaact ctacaatgga tcccgaataa acacacataa ataaacaaaa
     7021 tggtcgtaat taacggggtc aaatatgcct gtgaaacgtg tatcaggggt cacagggcgg
     7081 cgcagtgtac tcacactgat ggtccgctac agatgatcag acgcaaggga agaccatcga
     7141 ccacatgtgg ccattgtaaa gagctgagaa gaaccaagaa cttcaaccca tccggtgggt
     7201 gcatgtgtgc ctctgcacga cggccagctg ttggcagcaa ggaagatgaa acacgatgtc
     7261 gttgtgatga gggtgaacct tgtaaatgtc ataccaagag gaaaagcagc cggaaatcaa
     7321 agggagggtc atgccacaga agggcaaatg atgaagcagc gcatgtcaat ggtctcggta
     7381 ttgcagatct ggacgttctt ttgggcctaa atggtcgctc gtcggatgta gacatgacaa
     7441 ccacattgcc gagtttgaag ccacctctgc aaaacggaga aattaaggcc gacagcattg
     7501 acaatcttga tttggcttcc ctcgatccgc ttgagcaaag ccctagtata tctatggaac
     7561 ctgttagtat caatgaaaca ggaagcgcat atacaactac gaacacagca ctaaacgata
     7621 ttgacattcc attctccatc aatgagttga acgagctata caaacaagta tcttcgcata
     7681 actcacattc a
//
