LOCUS       Exported                7897 bp DNA     circular SYN 23-AUG-2022
DEFINITION  .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7897)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Aug 23, 2022 from SnapGene Viewer 6.0.2
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..7897
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     1..20
                     /label=Forward primer
                     /note="color: black; sequence: caagcggccgccgctgctgc; added:
                     2022-08-23"
     CDS             6..944
                     /label=HALOTag
                     /note="color: #ff00ff; direction: RIGHT"
     primer_bind     7..17
                     /label=Forward primer
                     /note="color: black; sequence: caagcggccgccgctgctgc; added:
                     2022-08-23"
     misc_feature    947..1346
                     /label=pTRP1
                     /note="color: #00ffff"
     CDS             1347..2021
                     /label=TRP1
                     /note="color: #00ffff; direction: RIGHT"
     primer_bind     complement(2000..2021)
                     /label=Reverse primer
                     /note="color: black; sequence: ctatttcttagcatttttgacg; 
                     added: 2022-08-23"
     misc_feature    2028..2284
                     /label=3' of ACE1 ORF
                     /note="color: #a6acb3"
     promoter        complement(2298..2317)
                     /label=T7_P
                     /note="T7 promoter"
                     /note="color: #ffffff; direction: LEFT"
     rep_origin      complement(2617..2716)
                     /direction=LEFT
                     /label=F1_ORI
                     /note="origin of replication"
                     /note="color: #999999"
     CDS             complement(3201..3860)
                     /label=HIS3
                     /note="HIS3 imidazoleglycerolphosphate dehydratase"
                     /note="color: #999999; direction: LEFT"
     CDS             4553..5413
                     /label=AmpR
                     /note="bla beta-lactamase, ampR ampicillin resistance"
                     /note="color: #999999; direction: RIGHT"
     rep_origin      complement(6171)
                     /direction=LEFT
                     /label=PMB1
                     /note="origin of replication"
                     /note="color: #ffff00"
     misc_feature    6646..6820
                     /label=5' of ACE1 ORF
                     /note="color: #a6acb3"
     misc_feature    6836..7193
                     /label=CAP2p
                     /note="Yeast promoter of CAP2 gene"
                     /note="color: #a6acb3"
     misc_feature    7200..7225
                     /label=Yeast leader
                     /note="Yeast translational leader sequence"
                     /note="color: #a6acb3"
     CDS             7226..7897
                     /label=ACE1
                     /note="ACE1 coding region, no stop signal"
                     /note="color: #999999; direction: RIGHT"
ORIGIN
        1 caagcggccg ccgctgctgc cgctgcagct gctgcagctg cgatgggttc cgaaatcggt
       61 actggctttc cattcgaccc ccattatgtg gaagtcctgg gcgagcgcat gcactacgtc
      121 gatgttggtc cgcgcgatgg cacccctgtg ctgttcctgc acggtaaccc gacctcctcc
      181 tacgtgtggc gcaacatcat cccgcatgtt gcaccgaccc atcgctgcat tgctccagac
      241 ctgatcggta tgggcaaatc cgacaaacca gacctgggtt atttcttcga cgaccacgtc
      301 cgcttcatgg atgccttcat cgaagccctg ggtctggaag aggtcgtcct ggtcattcac
      361 gactggggct ccgctctggg tttccactgg gccaagcgca atccagagcg cgtcaaaggt
      421 attgcattta tggagttcat ccgccctatc ccgacctggg acgaatggcc agaatttgcc
      481 cgcgagacct tccaggcctt ccgcaccacc gacgtcggcc gcaagctgat catcgatcag
      541 aacgttttta tcgagggtac gctgccgatg ggtgtcgtcc gcccgctgac tgaagtcgag
      601 atggaccatt accgcgagcc gttcctgaat cctgttgacc gcgagccact gtggcgcttc
      661 ccaaacgagc tgccaatcgc cggtgagcca gcgaacatcg tcgcgctggt cgaagaatac
      721 atggactggc tgcaccagtc ccctgtcccg aagctgctgt tctggggcac cccaggcgtt
      781 ctgatcccac cggccgaagc cgctcgcctg gccaaaagcc tgcctaactg caaggctgtg
      841 gacatcggcc cgggtctgaa tctgctgcaa gaagacaacc cggacctgat cggcagcgag
      901 atcgcgcgct ggctgtctac tctggagatt tccggtccga ccgcggaaac ggcagccccg
      961 atctaaaaga gctgacaggg aaatggtcag aaaaagaaac gtgcacccgc ccgtctggac
     1021 gcgccgctca cccgcacggc agagaccaat cagtaaaaat caacggttaa cgacattact
     1081 atatatataa tataggaagc atttaataga acagcatcgt aatatatgtg tactttgcag
     1141 ttatgacgcc agatggcagt agtggaagat attctttatt gaaaaatagc ttgtcacctt
     1201 acgtacaatc ttgatccgga gcttttcttt ttttgccgat taagaattcg gtcgaaaaaa
     1261 gaaaaggaga gggccaagag ggagggcatt ggtgactatt gagcacgtga gtatacgtga
     1321 ttaagcacac aaaggcagct tggagtatgt ctgttattaa tttcacaggt agttctggtc
     1381 cattggtgaa agtttgcggc ttgcagagca cagaggccgc agaatgtgct ctagattccg
     1441 atgctgactt gctgggtatt atatgtgtgc ccaatagaaa gagaacaatt gacccggtta
     1501 ttgcaaggaa aatttcaagt cttgtaaaag catataaaaa tagttcaggc actccgaaat
     1561 acttggttgg cgtgtttcgt aatcaaccta aggaggatgt tttggctctg gtcaatgatt
     1621 acggcattga tatcgtccaa ctgcatggag atgagtcgtg gcaagaatac caagagttcc
     1681 tcggtttgcc agttattaaa agactcgtat ttccaaaaga ctgcaacata ctactcagtg
     1741 cagcttcaca gaaacctcat tcgtttattc ccttgtttga ttcagaagca ggtgggacag
     1801 gtgaactttt ggattggaac tcgatttctg actgggttgg aaggcaagag agccccgaaa
     1861 gcttacattt tatgttagct ggtggactga cgccagaaaa tgttggtgat gcgcttagat
     1921 taaatggcgt tattggtgtt gatgtaagcg gaggtgtgga gacaaatggt gtaaaagact
     1981 ctaacaaaat agcaaatttc gtcaaaaatg ctaagaaata ggagctctgc atctggacat
     2041 ctatataaat atacatatac atatattggc ggttgtttgt tctcccggca agctggcagt
     2101 cgggccgaga ttcggataaa tttttccatt cgtatacata tacatagata tatacaatta
     2161 taaaatttgt ccaaggctgc acaggatacg taaccatcct aaagttttag gttttttaac
     2221 tttttttctg tctcttcaat tgtcgaattg cttattctat cctttctgga gttcgtattt
     2281 aaatgagctc caattcgccc tatagtgagt cgtattacaa ttcactggcc gtcgttttac
     2341 aacgtcgtga ctgggaaaac cctggcgtta cccaacttaa tcgccttgca gcacatcccc
     2401 ccttcgccag ctggcgtaat agcgaagagg cccgcaccga tcgcccttcc caacagttgc
     2461 gcagcctgaa tggcgaatgg cgcgacgcgc cctgtagcgg cgcattaagc gcggcgggtg
     2521 tggtggttac gcgcagcgtg accgctacac ttgccagcgc cctagcgccc gctcctttcg
     2581 ctttcttccc ttcctttctc gccacgttcg ccggctttcc ccgtcaagct ctaaatcggg
     2641 ggctcccttt agggttccga tttagtgctt tacggcacct cgaccccaaa aaacttgatt
     2701 agggtgatgg ttcacgtagt gggccatcgc cctgatagac ggtttttcgc cctttgacgt
     2761 tggagtccac gttctttaat agtggactct tgttccaaac tggaacaaca ctcaacccta
     2821 tctcggtcta ttcttttgat ttataaggga ttttgccgat ttcggcctat tggttaaaaa
     2881 atgagctgat ttaacaaaaa tttaacgcga attttaacaa aatattaacg tttacaattt
     2941 cctgatgcgg tattttctcc ttacgcatct gtgcggtatt tcacaccgca tatgatccgt
     3001 cgagttcaag agaaaaaaaa agaaaaagca aaaagaaaaa aggaaagcgc gcctcgttca
     3061 gaatgacacg tatagaatga tgcattacct tgtcatcttc agtatcatac tgttcgtata
     3121 catacttact gacattcata ggtatacata tatacacatg tatatatatc gtatgctgca
     3181 gctttaaata atcggtgtca ctacataaga acacctttgg tggagggaac atcgttggta
     3241 ccattgggcg aggtggcttc tcttatggca accgcaagag ccttgaacgc actctcacta
     3301 cggtgatgat cattcttgcc tcgcagacaa tcaacgtgga gggtaattct gctagcctct
     3361 gcaaagcttt caagaaaatg cgggatcatc tcgcaagaga gatctcctac tttctccctt
     3421 tgcaaaccaa gttcgacaac tgcgtacggc ctgttcgaaa gatctaccac cgctctggaa
     3481 agtgcctcat ccaaaggcgc aaatcctgat ccaaaccttt ttactccacg cgccagtagg
     3541 gcctctttaa aagcttgacc gagagcaatc ccgcagtctt cagtggtgtg atggtcgtct
     3601 atgtgtaagt caccaatgca ctcaacgatt agcgaccagc cggaatgctt ggccagagca
     3661 tgtatcatat ggtccagaaa ccctatacct gtgtggacgt taatcacttg cgattgtgtg
     3721 gcctgttctg ctactgcttc tgcctctttt tctgggaaga tcgagtgctc tatcgctagg
     3781 ggaccaccct ttaaagagat cgcaatctga atcttggttt catttgtaat acgctttact
     3841 agggctttct gctctgtcat ctttgccttc gtttatcttg cctgctcatt ttttagtata
     3901 ttcttcgaag aaatcacatt actttatata atgtataatt cattatgtga taatgccaat
     3961 cgctaagaaa aaaaaagagt catccgctag gtggaaaaaa aaaaatgaaa atcattaccg
     4021 aggcataaaa aaatatagag tgtactagag gaggccaaga gtaatagaaa aagaaaattg
     4081 cgggaaagga ctgtgttatg acttccctga ctaatgccgt gttcaaacga tacctggcag
     4141 tgactcctag cgctcaccaa gctcttaaaa cggaattatg gtgcactctc agtacaatct
     4201 gctctgatgc cgcatagtta agccagcccc gacacccgcc aacacccgct gacgcgccct
     4261 gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct
     4321 gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gagacgaaag ggcctcgtga
     4381 tacgcctatt tttataggtt aatgtcatga taataagacg tcaggtggca cttttcgggg
     4441 aaatgtgcgc ggaaccccta tttgtttatt tttctaaata cattcaaata tgtatccgct
     4501 catgagacaa taaccctgat aaatgcttca ataatattga aaaaggaaga gtatgagtat
     4561 tcaacatttc cgtgtcgccc ttattccctt ttttgcggca ttttgccttc ctgtttttgc
     4621 tcacccagaa acgctggtga aagtaaaaga tgctgaagat cagttgggtg cacgagtggg
     4681 ttacatcgaa ctggatctca acagcggtaa gatccttgag agttttcgcc ccgaagaacg
     4741 ttttccaatg atgagcactt ttaaagttct gctatgtggc gcggtattat cccgtattga
     4801 cgccgggcaa gagcaactcg gtcgccgcat acactattct cagaatgact tggttgagta
     4861 ctcaccagtc acagaaaagc atcttacgga tggcatgaca gtaagagaat tatgcagtgc
     4921 tgccataacc atgagtgata acactgcggc caacttactt ctgacaacga tcggaggacc
     4981 gaaggagcta accgcttttt ttcacaacat gggggatcat gtaactcgcc ttgatcgttg
     5041 ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt gacaccacga tgcctgtagc
     5101 aatggcaaca acgttgcgca aactattaac tggcgaacta cttactctag cttcccggca
     5161 acaattaata gactggatgg aggcggataa agttgcagga ccacttctgc gctcggccct
     5221 tccggctggc tggtttattg ctgataaatc tggagccggt gagcgtgggt ctcgcggtat
     5281 cattgcagca ctggggccag atggtaagcc ctcccgtatc gtagttatct acacgacggg
     5341 cagtcaggca actatggatg aacgaaatag acagatcgct gagataggtg cctcactgat
     5401 taagcattgg taactgtcag accaagttta ctcatatata ctttagattg atttaaaact
     5461 tcatttttaa tttaaaagga tctaggtgaa gatccttttt gataatctca tgaccaaaat
     5521 cccttaacgt gagttttcgt tccactgagc gtcagacccc gtagaaaaga tcaaaggatc
     5581 ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct
     5641 accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga aggtaactgg
     5701 cttcagcaga gcgcagatac caaatactgt ccttctagtg tagccgtagt taggccacca
     5761 cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtggc
     5821 tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat agttaccgga
     5881 taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct tggagcgaac
     5941 gacctacacc gaactgagat acctacagcg tgagcattga gaaagcgcca cgcttcccga
     6001 agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag
     6061 ggagcttcca ggggggaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg
     6121 acttgagcgt cgatttttgt gatgctcgtc aggggggccg agcctatgga aaaacgccag
     6181 caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca tgttctttcc
     6241 tgcgttatcc cctgattctg tggataaccg tattaccgcc tttgagtgag ctgataccgc
     6301 tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg aagagcgccc
     6361 aatacgcaaa ccgcctctcc ccgcgcgttg gccgattcat taatgcagct ggcacgacag
     6421 gtttcccgac tggaaagcgg gcagtgagcg caacgcaatt aatgtgagtt acctcactca
     6481 ttaggcaccc caggctttac actttatgct tccggctcct atgttgtgtg gaattgtgag
     6541 cggataacaa tttcacacag gaaacagcta tgaccatgat tacgccaagc tcggaattaa
     6601 ccctcactaa agggaacaaa agctggtacc gggccccccc tcgagattta aatcgccaaa
     6661 acgaggcgaa gtcgccaggg tgcgcgggca cgcagggtaa gagccgcaag gcctggttcg
     6721 gtaagtatta agctgtggac gaaatagcag tagccatggc gatgtaattt attagacggc
     6781 ggcttgataa aagaggactg ataatcagtg tattcacaga gtcgacggta tcgatgtaga
     6841 cgtattaaag ctcaatgatc ctacaagaca ttaagtaaaa aggggaagcg atcttaaatc
     6901 cacatgaatt tcctttctct ttttcttctt cttctgttcc ctttttcttt cttcttcctt
     6961 caacgtctac gtaaatataa tgtataacat ctccactttc ctttccctta tagcgttatg
     7021 ttacctgttt aaactctaaa ttcttcatct actactgctg ttactacatg tatatatgtt
     7081 catttttttt tttttttttc accacggaaa acgtaaaaat gagagcattt gtaacaaata
     7141 aaggagaact gttcaatcaa gtgacataca aaaactctct ccaactctac aatggatccc
     7201 gaataaacac acataaataa acaaaatggt cgtaattaac ggggtcaaat atgcctgtga
     7261 aacgtgtatc aggggtcaca gggcggcgca gtgtactcac actgatggtc cgctacagat
     7321 gatcagacgc aagggaagac catcgaccac atgtggccat tgtaaagagc tgagaagaac
     7381 caagaacttc aacccatccg gtgggtgcat gtgtgcctct gcacgacggc cagctgttgg
     7441 cagcaaggaa gatgaaacac gatgtcgttg tgatgagggt gaaccttgta aatgtcatac
     7501 caagaggaaa agcagccgga aatcaaaggg agggtcatgc cacagaaggg caaatgatga
     7561 agcagcgcat gtcaatggtc tcggtattgc agatctggac gttcttttgg gcctaaatgg
     7621 tcgctcgtcg gatgtagaca tgacaaccac attgccgagt ttgaagccac ctctgcaaaa
     7681 cggagaaatt aaggccgaca gcattgacaa tcttgatttg gcttccctcg atccgcttga
     7741 gcaaagccct agtatatcta tggaacctgt tagtatcaat gaaacaggaa gcgcatatac
     7801 aactacgaac acagcactaa acgatattga cattccattc tccatcaatg agttgaacga
     7861 gctatacaaa caagtatctt cgcataactc acattca
//
