LOCUS       mfdpsl4                 7516 bp    DNA     circular SYN 20-AUG-2022
DEFINITION  .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
COMMENT     Annotated with pLannotate v1.2.0
FEATURES             Location/Qualifiers
     CDS             complement(470..1309)
                     /note="pLannotate"
                     /label="RSF1010 RepA"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="CDS"
     rep_origin      4459..4853
                     /note="pLannotate"
                     /label="RSF1010 oriV"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="rep_origin"
     CDS             complement(6360..7016)
                     /note="pLannotate"
                     /label="cat"
                     /database="swissprot"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="CDS"
     CDS             complement(1823..3949)
                     /note="pLannotate"
                     /label="mobA"
                     /database="swissprot"
                     /identity="99.9"
                     /match_length="100.0"
                     /fragment="False"
                     /other="CDS"
     oriT            complement(4030..4117)
                     /note="pLannotate"
                     /label="RSF1010 oriT"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="oriT"
     CDS             complement(join(7148..7516,1..483))
                     /note="pLannotate"
                     /label="RSF1010 RepC (fragment)"
                     /database="snapgene"
                     /identity="99.8"
                     /match_length="100.1"
                     /fragment="True"
                     /other="CDS"
     terminator      6213..6284
                     /note="pLannotate"
                     /label="rrnB T1 terminator"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="terminator"
     CDS             5527..6193
                     /note="pLannotate"
                     /label="mRFP1 (fragment)"
                     /database="snapgene"
                     /identity="99.8"
                     /match_length="98.4"
                     /fragment="True"
                     /other="CDS"
     terminator      6300..6327
                     /note="pLannotate"
                     /label="T7Te terminator"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="terminator"
     promoter        complement(7017..7114)
                     /note="pLannotate"
                     /label="cat promoter"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="95.1"
                     /fragment="False"
                     /other="promoter"
     ncRNA           complement(1301..1375)
                     /note="pLannotate"
                     /label="Plasmid R1162"
                     /database="Rfam"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="ncRNA"
     CDS             4148..4429
                     /note="pLannotate"
                     /label="mobC"
                     /database="swissprot"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="CDS"
ORIGIN
        1 cagcagccga aacccctgcc gcttgcggcc attctgggcg atgatggata ccttccaaag
       61 gcgctcgatg cagtcctgta tgtgcttgag cgccccacca ctatcgacct ctgccccgat
      121 ttcctttgcc agcgcccgat agctaccttt gaccaccatg gcatcagcgg tgacggcctc
      181 ccacttgggt tccaggaaca gccggagctg ccgtccgcct tcggtcttgg gttccgggcc
      241 aagcactagg ccattaggcc cagccatggc caccagccct tgcaggatgc gcagatcatc
      301 agcgcccagc ggctccgggc cgctgaactc gatccgcttg ccgtcgccgt agtcatacgt
      361 cacgtccagc ttgctgcgct tgcgctcgcc ccgcttgagg gcacggaaca ggccgggggc
      421 cagacagtgc gccgggtcgt gccggacgtg gctgaggctg tgcttgttct taggcttcac
      481 cacggggcac ccccttgctc ttgcgctgcc tctccagcac ggcgggcttg agcaccccgc
      541 cgtcatgccg cctgaaccac cgatcagcga acggtgcgcc atagttggcc ttgctcacac
      601 cgaagcggac gaagaaccgg cgctggtcgt cgtccacacc ccattcctcg gcctcggcgc
      661 tggtcatgct cgacaggtag gactgccagc ggatgttatc gaccagtacc gagctgcccc
      721 ggctggcctg ctgctggtcg cctgcgccca tcatggccgc gcccttgctg gcatggtgca
      781 ggaacacgat agagcacccg gtatcggcgg cgatggcctc catgcgaccg atgacctggg
      841 ccatggggcc gctggcgttt tcttcctcga tgtggaaccg gcgcagcgtg tccagcacca
      901 tcaggcggcg gccctcggcg gcgcgcttga ggccgtcgaa ccactccggg gccatgatgt
      961 tgggcaggct gccgatcagc ggctggatca gcaggccgtc agccacggct tgccgttcct
     1021 cggcgctgag gtgcgcccca agggcgtgca ggcggtgatg aatggcggtg ggcgggtctt
     1081 cggcgggcag gtagatcacc gggccggtgg gcagttcgcc cacctccagc agatccggcc
     1141 cgcctgcaat ctgtgcggcc agttgcaggg ccagcatgga tttaccggca ccaccgggcg
     1201 acaccagcgc cccgaccgta ccggccacca tgttgggcaa aacgtagtcc agcggtggcg
     1261 gcgctgctgc gaacgcctcc agaatattga taggcttatg ggtagccatt gattgcctcc
     1321 tttgcaggca gttggtggtt aggcgctggc ggggtcacta cccccgccct gcgccgctct
     1381 gagttcttcc aggcactcgc gcagcgcctc gtattcgtcg tcggtcagcc agaacttgcg
     1441 ctgacgcatc cctttggcct tcatgcgctc ggcatatcgc gcttggcgta cagcgtcagg
     1501 gctggccagc aggtcgccgg tctgcttgtc cttttggtct ttcatatcag tcaccgagaa
     1561 acttgccggg gccgaaaggc ttgtcttcgc ggaacaagga caaggtgcag ccgtcaaggt
     1621 taaggctggc catatcagcg actgaaaagc ggccagcctc ggccttgttt gacgtataac
     1681 caaagccacc gggcaaccaa tagcccttgt cacttttgat caggtagacc gaccctgaag
     1741 cgcttttttc gtattccata aaaccccctt ctgtgcgtga gtactcatag tataacaggc
     1801 gtgagtacca acgcaagcac tacatgctga aatctggccc gcccctgtcc atgcctcgct
     1861 ggcggggtgc cggtgcccgt gccagctcgg cccgcgcaag ctggacgctg ggcagaccca
     1921 tgaccttgct gacggtgcgc tcgatgtaat ccgcttcgtg gccgggcttg cgctctgcca
     1981 gcgctgggct ggcctcggcc atggccttgc cgatttcctc ggcactgcgg ccccggctgg
     2041 ccagcttctg cgcggcgata aagtcgcact tgctgaggtc atcaccgaag cgcttgacca
     2101 gcccggccat ctcgctgcgg tactcgtcca gcgccgtgcg ccggtggcgg ctaagctgcc
     2161 gctcgggcag ttcgaggctg gccagcctgc gggccttctc ctgctgccgc tgggcctgct
     2221 cgatctgctg gccagcctgc tgcaccagcg ccgggccagc ggtggcggtc ttgcccttgg
     2281 attcacgcag cagcacccac ggctgataac cggcgcgggt ggtgtgcttg tccttgcggt
     2341 tggtgaagcc cgccaagcgg ccatagtggc ggctgtcggc gctggccggg tcggcgtcgt
     2401 actcgctggc cagcgtccgg gcaatctgcc cccgaagttc accgcctgcg gcgtcggcca
     2461 ccttgaccca tgcctgatag ttcttcgggc tggtttccac taccagggca ggctcccggc
     2521 cctcggcttt catgtcatcg aggtcaaact cgctgaggtc gtccaccagc accagaccat
     2581 gccgctcctg ctcggcgggc ctgatataca cgtcattgcc cagggcattc atccgcttga
     2641 gccatggcgt gttctggagc acttcggcgg ctgaccattc ccggttcatc atctggccgg
     2701 tggtggcgtc cctgacgccg atatcgaagc gctcacagcc catggccttg agctgtcggc
     2761 ctatggcctg caaagtcctg tcgttcttca tcgggccacc aagcgcagcc agatcgagcc
     2821 gtcctcggtt gtcagtggcg tcaggtcgag caagagcaac gatgcgatca gcagcaccac
     2881 cgtaggcatc atggaagcca gcatcacggt tagccatagc ttccagtgcc acccccgcga
     2941 cgcgctccgg gcgctctgcg cggcgctgct cacctcggcg gctacctccc gcaactcttt
     3001 ggccagctcc acccatgccg cccctgtctg gcgctgggct ttcagccact ccgccgcctg
     3061 cgcctcgctg gcctgctggg tctggctcat gacctgccgg gcttcgtcgg ccagtgtcgc
     3121 catgctctgg gccagcggtt cgatctgctc cgctaactcg ttgatgcctc tggatttctt
     3181 cactctgtcg attgcgttca tggtctattg cctcccggta ttcctgtaag tcgatgatct
     3241 gggcgttggc ggtgtcgatg ttcagggcca cgtctgcccg gtcggtgcgg atgccccggc
     3301 cttccatctc caccacgttc ggccccaggt gaacaccggg caggcgctcg atgccctgcg
     3361 cctcaagtgt tctgtggtca atgcgggcgt cgtggccagc ccgctctaat gcccggttgg
     3421 catggtcggc ccatgcctcg cgggtctgct caagccatgc cttgggcttg agcgcttcgg
     3481 tcttctgtgc cccgcccttc tccggggtct tgccgttgta ccgcttgaac cactgagcgg
     3541 cgggccgctc gatgccgtca ttgatccgct cggagatcat caggtggcag tgcgggttct
     3601 cgccgccacc ggcatggatg gccagcgtat acggcaggcg ctcggcaccg gtcaggtgct
     3661 gggcgaactc ggacgccagc gccttctgct ggtcgagggt cagctcgacc ggcagggcaa
     3721 attcgacctc cttgaacagc cgcccattgg cgcgttcata caggtcggca gcatcccagt
     3781 agtcggcggg ccgctcgacg aactccggca tgtgcccgga ttcggcgtgc aagacttcat
     3841 ccatgtcgcg ggcatacttg ccttcgcgct ggatgtagtc ggccttggcc ctggccgatt
     3901 ggccgcccga cctgctgccg gttttcgccg taaggtgata aatcgccatg ctgcctcgct
     3961 gttgcttttg cttttcggct ccatgcaatg gccctcggag agcgcaccgc ccgaagggtg
     4021 gccgttaggc cagtttctcg aagagaaacc ggtaagtgcg ccctccccta caaagtaggg
     4081 tcgggattgc cgccgctgtg cctccatgat agcctacgag acagcacatt aacaatgggg
     4141 tgtcaagatg gttaagggga gcaacaaggc ggcggatcgg ctggccaagc tcgaagaaca
     4201 acgagcgcga atcaatgccg aaattcagcg ggtgcgggca agggaacagc agcaagagcg
     4261 caagaacgaa acaaggcgca aggtgctggt gggggccatg attttggcca aggtgaacag
     4321 cagcgagtgg ccggaggatc ggctcatggc ggcaatggat gcgtaccttg aacgcgacca
     4381 cgaccgcgcc ttgttcggtc tgccgccacg ccagaaggat gagccgggct gaatgatcga
     4441 ccgagacagg ccctgcgggg ctgcacacgc gcccccaccc ttcgggtagg gggaaaggcc
     4501 gctaaagcgg ctaaaagcgc tccagcgtat ttctgcgggg tttggtgtgg ggtttagcgg
     4561 gctttgcccg cctttccccc tgccgcgcag cggtggggcg gtgtgtagcc tagcgcagcg
     4621 aatagaccag ctatccggcc tctggccggg catattgggc aagggcagca gcgccccaca
     4681 agggcgctga taaccgcgcc tagtggatta ttcttagata atcatggatg gatttttcca
     4741 acaccccgcc agcccccgcc cctgctgggt ttgcaggttt gggggcgtga cagttattgc
     4801 aggggttcgt gacagttatt gcaggggggc gtgacagtta ttgcaggggt tcgtgacagt
     4861 tagtacggga gtgacgggca ctggctggca atgtctagca acggcaggca tttcggctga
     4921 gggtaaaaga actttccgct aagcgataga ctgtatgtaa acacagtatt gcaaggacgc
     4981 ggaacatgcc tcatgtggcg gccaggacgg ccagccggga tcgggatact ggtcgttacc
     5041 agagccaccg acccgagcaa acccttctct atcagatcgt tgacgagtat tacccggcat
     5101 tcgctgcgct tatggcagag cagggaaagg aattgccggg ctatgtgcaa cgggaatttg
     5161 aagaatttct ccaatgcggg cggctggagc atggctttct acgggttcgc tgcgagtctt
     5221 gccacgccga gcacctggtc gctttcagaa atcaatctaa agtatatatg agtaaacttg
     5281 gtctgacagg ccccttgaga ccagctttgg cagtttattc ttgacatgta gtgagggggc
     5341 tggtataatc acatagtact gttatacaga aacagaggag atattacata tggcttcctc
     5401 cgaagacgtt atcaggcccc ttgagaccag ctttggcagt ttattcttga catgtagtga
     5461 gggggctggt ataatcacat agtactgtta tacagaaaca gaggagatat tacatatggc
     5521 ttcctccgaa gacgttatca aagagttcat gcgtttcaaa gttcgtatgg aaggttccgt
     5581 taacggtcac gagttcgaaa tcgaaggtga aggtgaaggt cgtccgtacg aaggtaccca
     5641 gaccgctaaa ctgaaagtta ccaaaggtgg tccgctgccg ttcgcttggg acatcctgtc
     5701 cccgcagttc cagtacggtt ccaaagctta cgttaaacac ccggctgaca tcccggacta
     5761 cctgaaactg tccttcccgg aaggtttcaa atgggaacgt gttatgaact tcgaagacgg
     5821 tggtgttgtt accgttaccc aggactcctc cctgcaagac ggtgagttca tctacaaagt
     5881 taaactgcgt ggtaccaact tcccgtccga cggtccggtt atgcagaaaa aaaccatggg
     5941 ttgggaagct tccaccgaac gtatgtaccc ggaagacggt gctctgaaag gtgaaatcaa
     6001 aatgcgtctg aaactgaaag acggtggtca ctacgacgct gaagttaaaa ccacctacat
     6061 ggctaaaaaa ccggttcagc tgccgggtgc ttacaaaacc gacatcaaac tggacatcac
     6121 ctcccacaac gaagactaca ccatcgttga acagtacgaa cgtgctgaag gtcgtcactc
     6181 caccggcgct taatataagg atctccaggc atcaaataaa acgaaaggct cagtcgaaag
     6241 actgggcctt tcgttttatc tgttgtttgt cggtgaacgc tctctactag agtcacactg
     6301 gctcaccttc gggtgggcct ttctgcgttt ataagtcggt ctcaccgagc ggccgcttac
     6361 gccccgccct gccactcatc gcagtactgt tgtaattcat taagcattct gccgacatgg
     6421 aagccatcac aaacggcatg atgaacctga atcgccagcg gcatcagcac cttgtcgcct
     6481 tgcgtataat atttgcccat ggtgaaaacg ggggcgaaga agttgtccat attggccacg
     6541 tttaaatcaa aactggtgaa actcacccag ggattggctg aaacgaaaaa catattctca
     6601 ataaaccctt tagggaaata ggccaggttt tcaccgtaac acgccacatc ttgcgaatat
     6661 atgtgtagaa actgccggaa atcgtcgtgg tattcactcc agagcgatga aaacgtttca
     6721 gtttgctcat ggaaaacggt gtaacaaggg tgaacactat cccatatcac cagctcaccg
     6781 tctttcattg ccatacgaaa ttccggatga gcattcatca ggcgggcaag aatgtgaata
     6841 aaggccggat aaaacttgtg cttatttttc tttacggtct ttaaaaaggc cgtaatatcc
     6901 agctgaacgg tctggttata ggtacattga gcaactgact gaaatgcctc aaaatgttct
     6961 ttacgatgcc attgggatat atcaacggtg gtatatccag tgattttttt ctccatttta
     7021 gcttccttag ctcctgaaaa tctcgataac tcaaaaaata cgcccggtag tgatcttatt
     7081 tcattatggt gaaagttgga acctcttacg tgcccgatca atcatgacca aaatccctta
     7141 acgtgagtca gcctgccgcc ttgggccggg tgatgtcgta cttgcccgcc gcgaactcgg
     7201 ttaccgtcca gcccagcgcg accagctccg gcaacgcctc gcgcacccgc tggcggcgct
     7261 tgcgcatggt cgaaccactg gcctctgacg gccagacata gccgcacaag gtatctatgg
     7321 aagccttgcc ggttttgccg gggtcgatcc agccacacag ccgctggtgc agcaggcggg
     7381 cggtttcgct gtccagcgcc cgcacctcgt ccatgctgat gcgcacatgc tggccgccac
     7441 ccatgacggc ctgcgcgatc aaggggttca gggccacgta caggcgcccg tccgcctcgt
     7501 cgctggcgta ctccga
//
