LOCUS       mfdpsl7                 7642 bp    DNA     circular SYN 20-AUG-2022
DEFINITION  .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
COMMENT     Annotated with pLannotate v1.2.0
FEATURES             Location/Qualifiers
     CDS             2324..3295
                     /note="pLannotate"
                     /label="RSF1010 RepB"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="CDS"
     CDS             3806..4645
                     /note="pLannotate"
                     /label="RSF1010 RepA"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="CDS"
     CDS             complement(6693..7367)
                     /note="pLannotate"
                     /label="mRFP1"
                     /database="fpbase"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="CDS"
     rep_origin      complement(262..656)
                     /note="pLannotate"
                     /label="RSF1010 oriV"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="rep_origin"
     promoter        5536..5640
                     /note="pLannotate"
                     /label="AmpR promoter"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="promoter"
     oriT            998..1085
                     /note="pLannotate"
                     /label="RSF1010 oriT"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="oriT"
     CDS             5641..6500
                     /note="pLannotate"
                     /label="AmpR (fragment)"
                     /database="snapgene"
                     /identity="99.5"
                     /match_length="99.9"
                     /fragment="True"
                     /other="CDS"
     CDS             4632..5483
                     /note="pLannotate"
                     /label="RSF1010 RepC (fragment)"
                     /database="snapgene"
                     /identity="99.8"
                     /match_length="100.1"
                     /fragment="True"
                     /other="CDS"
     terminator      complement(6599..6670)
                     /note="pLannotate"
                     /label="rrnB T1 terminator"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="terminator"
     terminator      complement(6556..6583)
                     /note="pLannotate"
                     /label="T7Te terminator"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="terminator"
     ncRNA           3738..3812
                     /note="pLannotate"
                     /label="Plasmid R1162"
                     /database="Rfam"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="ncRNA"
     CDS             1914..2324
                     /note="pLannotate"
                     /label="mobB"
                     /database="swissprot"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="CDS"
     CDS             complement(686..967)
                     /note="pLannotate"
                     /label="mobC"
                     /database="swissprot"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="CDS"
ORIGIN
        1 cataagcgca gcgaatgccg ggtaatactc gtcaacgatc tgatagagaa gggtttgctc
       61 gggtcggtgg ctctggtaac gaccagtatc ccgatcccgg ctggccgtcc tggccgccac
      121 atgaggcatg ttccgcgtcc ttgcaatact gtgtttacat acagtctatc gcttagcgga
      181 aagttctttt accctcagcc gaaatgcctg ccgttgctag acattgccag ccagtgcccg
      241 tcactcccgt actaactgtc acgaacccct gcaataactg tcacgccccc ctgcaataac
      301 tgtcacgaac ccctgcaata actgtcacgc ccccaaacct gcaaacccag caggggcggg
      361 ggctggcggg gtgttggaaa aatccatcca tgattatcta agaataatcc actaggcgcg
      421 gttatcagcg cccttgtggg gcgctgctgc ccttgcccaa tatgcccggc cagaggccgg
      481 atagctggtc tattcgctgc gctaggctac acaccgcccc accgctgcgc ggcaggggga
      541 aaggcgggca aagcccgcta aaccccacac caaaccccgc agaaatacgc tggagcgctt
      601 ttagccgctt tagcggcctt tccccctacc cgaagggtgg gggcgcgtgt gcagccccgc
      661 agggcctgtc tcggtcgatc attcagcccg gctcatcctt ctggcgtggc ggcagaccga
      721 acaaggcgcg gtcgtggtcg cgttcaaggt acgcatccat tgccgccatg agccgatcct
      781 ccggccactc gctgctgttc accttggcca aaatcatggc ccccaccagc accttgcgcc
      841 ttgtttcgtt cttgcgctct tgctgctgtt cccttgcccg cacccgctga atttcggcat
      901 tgattcgcgc tcgttgttct tcgagcttgg ccagccgatc cgccgccttg ttgctcccct
      961 taaccatctt gacaccccat tgttaatgtg ctgtctcgta ggctatcatg gaggcacagc
     1021 ggcggcaatc ccgaccctac tttgtagggg agggcgcact taccggtttc tcttcgagaa
     1081 actggcctaa cggccaccct tcgggcggtg cgctctccga gggccattgc atggagccga
     1141 aaagcaaaag caacagcgag gcagcatggc gatttatcac cttacggcga aaaccggcag
     1201 caggtcgggc ggccaatcgg ccagggccaa ggccgactac atccagcgcg aaggcaagta
     1261 tgcccgcgac atggatgaag tcttgcacgc cgaatccggg cacatgccgg agttcgtcga
     1321 gcggcccgcc gactactggg atgctgccga cctgtatgaa cgcgccaatg ggcggctgtt
     1381 caaggaggtc gaatttgccc tgccggtcga gctgaccctc gaccagcaga aggcgctggc
     1441 gtccgagttc gcccagcacc tgaccggtgc cgagcgcctg ccgtatacgc tggccatcca
     1501 tgccggtggc ggcgagaacc cgcactgcca cctgatgatc tccgagcgga tcaatgacgg
     1561 catcgagcgg cccgccgctc agtggttcaa gcggtacaac ggcaagaccc cggagaaggg
     1621 cggggcacag aagaccgaag cgctcaagcc caaggcatgg cttgagcaga cccgcgaggc
     1681 atgggccgac catgccaacc gggcattaga gcgggctggc cacgacgccc gcattgacca
     1741 cagaacactt gaggcgcagg gcatcgagcg cctgcccggt gttcacctgg ggccgaacgt
     1801 ggtggagatg gaaggccggg gcatccgcac cgaccgggca gacgtggccc tgaacatcga
     1861 caccgccaac gcccagatca tcgacttaca ggaataccgg gaggcaatag accatgaacg
     1921 caatcgacag agtgaagaaa tccagaggca tcaacgagtt agcggagcag atcgaaccgc
     1981 tggcccagag catggcgaca ctggccgacg aagcccggca ggtcatgagc cagacccagc
     2041 aggccagcga ggcgcaggcg gcggagtggc tgaaagccca gcgccagaca ggggcggcat
     2101 gggtggagct ggccaaagag ttgcgggagg tagccgccga ggtgagcagc gccgcgcaga
     2161 gcgcccggag cgcgtcgcgg gggtggcact ggaagctatg gctaaccgtg atgctggctt
     2221 ccatgatgcc tacggtggtg ctgctgatcg catcgttgct cttgctcgac ctgacgccac
     2281 tgacaaccga ggacggctcg atctggctgc gcttggtggc ccgatgaaga acgacaggac
     2341 tttgcaggcc ataggccgac agctcaaggc catgggctgt gagcgcttcg atatcggcgt
     2401 cagggacgcc accaccggcc agatgatgaa ccgggaatgg tcagccgccg aagtgctcca
     2461 gaacacgcca tggctcaagc ggatgaatgc ccagggcaat gacgtgtata tcaggcccgc
     2521 cgagcaggag cggcatggtc tggtgctggt ggacgacctc agcgagtttg acctggatga
     2581 catgaaagcc gagggccggg agcctgccct ggtagtggaa accagcccga agaactatca
     2641 ggcatgggtc aaggtggccg acgccgcagg cggtgaactt cgggggcaga ttgcccggac
     2701 gctggccagc gagtacgacg ccgacccggc cagcgccgac agccgccact atggccgctt
     2761 ggcgggcttc accaaccgca aggacaagca caccacccgc gccggttatc agccgtgggt
     2821 gctgctgcgt gaatccaagg gcaagaccgc caccgctggc ccggcgctgg tgcagcaggc
     2881 tggccagcag atcgagcagg cccagcggca gcaggagaag gcccgcaggc tggccagcct
     2941 cgaactgccc gagcggcagc ttagccgcca ccggcgcacg gcgctggacg agtaccgcag
     3001 cgagatggcc gggctggtca agcgcttcgg tgatgacctc agcaagtgcg actttatcgc
     3061 cgcgcagaag ctggccagcc ggggccgcag tgccgaggaa atcggcaagg ccatggccga
     3121 ggccagccca gcgctggcag agcgcaagcc cggccacgaa gcggattaca tcgagcgcac
     3181 cgtcagcaag gtcatgggtc tgcccagcgt ccagcttgcg cgggccgagc tggcacgggc
     3241 accggcaccc cgccagcgag gcatggacag gggcgggcca gatttcagca tgtagtgctt
     3301 gcgttggtac tcacgcctgt tatactatga gtactcacgc acagaagggg gttttatgga
     3361 atacgaaaaa agcgcttcag ggtcggtcta cctgatcaaa agtgacaagg gctattggtt
     3421 gcccggtggc tttggttata cgtcaaacaa ggccgaggct ggccgctttt cagtcgctga
     3481 tatggccagc cttaaccttg acggctgcac cttgtccttg ttccgcgaag acaagccttt
     3541 cggccccggc aagtttctcg gtgactgata tgaaagacca aaaggacaag cagaccggcg
     3601 acctgctggc cagccctgac gctgtacgcc aagcgcgata tgccgagcgc atgaaggcca
     3661 aagggatgcg tcagcgcaag ttctggctga ccgacgacga atacgaggcg ctgcgcgagt
     3721 gcctggaaga actcagagcg gcgcagggcg ggggtagtga ccccgccagc gcctaaccac
     3781 caactgcctg caaaggaggc aatcaatggc tacccataag cctatcaata ttctggaggc
     3841 gttcgcagca gcgccgccac cgctggacta cgttttgccc aacatggtgg ccggtacggt
     3901 cggggcgctg gtgtcgcccg gtggtgccgg taaatccatg ctggccctgc aactggccgc
     3961 acagattgca ggcgggccgg atctgctgga ggtgggcgaa ctgcccaccg gcccggtgat
     4021 ctacctgccc gccgaagacc cgcccaccgc cattcatcac cgcctgcacg cccttggggc
     4081 gcacctcagc gccgaggaac ggcaagccgt ggctgacggc ctgctgatcc agccgctgat
     4141 cggcagcctg cccaacatca tggccccgga gtggttcgac ggcctcaagc gcgccgccga
     4201 gggccgccgc ctgatggtgc tggacacgct gcgccggttc cacatcgagg aagaaaacgc
     4261 cagcggcccc atggcccagg tcatcggtcg catggaggcc atcgccgccg ataccgggtg
     4321 ctctatcgtg ttcctgcacc atgccagcaa gggcgcggcc atgatgggcg caggcgacca
     4381 gcagcaggcc agccggggca gctcggtact ggtcgataac atccgctggc agtcctacct
     4441 gtcgagcatg accagcgccg aggccgagga atggggtgtg gacgacgacc agcgccggtt
     4501 cttcgtccgc ttcggtgtga gcaaggccaa ctatggcgca ccgttcgctg atcggtggtt
     4561 caggcggcat gacggcgggg tgctcaagcc cgccgtgctg gagaggcagc gcaagagcaa
     4621 gggggtgccc cgtggtgaag cctaagaaca agcacagcct cagccacgtc cggcacgacc
     4681 cggcgcactg tctggccccc ggcctgttcc gtgccctcaa gcggggcgag cgcaagcgca
     4741 gcaagctgga cgtgacgtat gactacggcg acggcaagcg gatcgagttc agcggcccgg
     4801 agccgctggg cgctgatgat ctgcgcatcc tgcaagggct ggtggccatg gctgggccta
     4861 atggcctagt gcttggcccg gaacccaaga ccgaaggcgg acggcagctc cggctgttcc
     4921 tggaacccaa gtgggaggcc gtcaccgctg atgccatggt ggtcaaaggt agctatcggg
     4981 cgctggcaaa ggaaatcggg gcagaggtcg atagtggtgg ggcgctcaag cacatacagg
     5041 actgcatcga gcgcctttgg aaggtatcca tcatcgccca gaatggccgc aagcggcagg
     5101 ggtttcggct gctgtcggag tacgccagcg acgaggcgga cgggcgcctg tacgtggccc
     5161 tgaacccctt gatcgcgcag gccgtcatgg gtggcggcca gcatgtgcgc atcagcatgg
     5221 acgaggtgcg ggcgctggac agcgaaaccg cccgcctgct gcaccagcgg ctgtgtggct
     5281 ggatcgaccc cggcaaaacc ggcaaggctt ccatagatac cttgtgcggc tatgtctggc
     5341 cgtcagaggc cagtggttcg accatgcgca agcgccgcca gcgggtgcgc gaggcgttgc
     5401 cggagctggt cgcgctgggc tggacggtaa ccgagttcgc ggcgggcaag tacgacatca
     5461 cccggcccaa ggcggcaggc tgactcacgt taagggattt tggtcatgac aggtggcact
     5521 tttcggggaa atgtgcgcgg aacccctatt tgtttatttt tctaaataca ttcaaatatg
     5581 tatccgctca tgagacaata accctgataa atgcttcaat aatattgaaa aaggaagagt
     5641 atgagtattc aacatttccg tgtcgccctt attccctttt ttgcggcatt ttgccttcct
     5701 gtttttgctc acccagaaac gctggtgaaa gtaaaagatg ctgaagatca gttgggtgca
     5761 ctgagtgggt tacatcgaac tggatctcaa cagcggtaag atccttgaga gttttcgccc
     5821 cgaagaacgt tttccaatga tgagcacttt taaagttctg ctatgtggcg cggtattatc
     5881 ccgtattgac gccgggcaag agcaactcgg tcgccgcata cactattctc agaatgactt
     5941 ggttgagtac tcaccagtca cagaaaagca tcttacggat ggcatgacag taagagaatt
     6001 atgcagtgct gccataacca tgagtgataa cactgcggcc aacttacttc tgacaacgat
     6061 cggaggaccg aaggagctaa ccgctttttt gcacaacatg ggggatcatg taactcgcct
     6121 tgatcgttgg gaaccggagc tgaatgaagc cataccaaac gacgagcgtg acaccacgat
     6181 gcctgtagca atggcaacaa cgttgcgcaa actattaact ggcgaactac ttactctagc
     6241 ttcccggcaa caattaatag actggatgga ggcggataaa gttgcaggac cacttctgcg
     6301 ctcggccctt ccggctggct ggtttattgc tgataaatct ggagccggtg agcgtgggtc
     6361 ccgcggtatc attgcagcac tggggccaga tggtaagccc tcccgtatcg tagttatcta
     6421 cacgacgggg agtcaggcaa ctatggatga acgaaataga cagatcgctg agataggtgc
     6481 ctcactgatt aagcattggt cagaattggt taattggttg taacacgcgg ccgctcggtg
     6541 agaccgactt ataaacgcag aaaggcccac ccgaaggtga gccagtgtga ctctagtaga
     6601 gagcgttcac cgacaaacaa cagataaaac gaaaggccca gtctttcgac tgagcctttc
     6661 gttttatttg atgcctggag atccttatat taagcgccgg tggagtgacg accttcagca
     6721 cgttcgtact gttcaacgat ggtgtagtct tcgttgtggg aggtgatgtc cagtttgatg
     6781 tcggttttgt aagcacccgg cagctgaacc ggttttttag ccatgtaggt ggttttaact
     6841 tcagcgtcgt agtgaccacc gtctttcagt ttcagacgca ttttgatttc acctttcaga
     6901 gcaccgtctt ccgggtacat acgttcggtg gaagcttccc aacccatggt ttttttctgc
     6961 ataaccggac cgtcggacgg gaagttggta ccacgcagtt taactttgta gatgaactca
     7021 ccgtcttgca gggaggagtc ctgggtaacg gtaacaacac caccgtcttc gaagttcata
     7081 acacgttccc atttgaaacc ttccgggaag gacagtttca ggtagtccgg gatgtcagcc
     7141 gggtgtttaa cgtaagcttt ggaaccgtac tggaactgcg gggacaggat gtcccaagcg
     7201 aacggcagcg gaccaccttt ggtaactttc agtttagcgg tctgggtacc ttcgtacgga
     7261 cgaccttcac cttcaccttc gatttcgaac tcgtgaccgt taacggaacc ttccatacga
     7321 actttgaaac gcatgaactc tttgataacg tcttcggagg aagccatatg taatatctcc
     7381 tctgtttctg tataacagta ctatgtgatt ataccagccc cctcactaca tgtcaagaat
     7441 aaactgccaa agctggtctc aaggggcctg tcagaccaag tttactcata tatactttag
     7501 attgatttct gaaagcgacc aggtgctcgg cgtggcaaga ctcgcagcga acccgtagaa
     7561 agccatgctc cagccgcccg cattggagaa attcttcaaa ttcccgttgc acatagcccg
     7621 gcaattcctt tccctgctct gc
//
