LOCUS       mfdpsl25                 4489 bp    DNA     circular SYN 20-AUG-2022
DEFINITION  .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
COMMENT     Annotated with pLannotate v1.2.0
FEATURES             Location/Qualifiers
     CDS             1335..2483
                     /note="pLannotate"
                     /label="trfA"
                     /database="snapgene"
                     /identity="99.7"
                     /match_length="100.0"
                     /fragment="False"
                     /other="CDS"
     CDS             2970..3785
                     /note="pLannotate"
                     /label="KanR"
                     /database="snapgene"
                     /identity="99.8"
                     /match_length="100.0"
                     /fragment="False"
                     /other="CDS"
     terminator      complement(3885..3956)
                     /note="pLannotate"
                     /label="rrnB T1 terminator"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="terminator"
     CDS             complement(join(3979..4489,1..152))
                     /note="pLannotate"
                     /label="amilCP"
                     /database="fpbase"
                     /identity="99.5"
                     /match_length="100.0"
                     /fragment="False"
                     /other="CDS"
     rep_origin      411..942
                     /note="pLannotate"
                     /label="oriV"
                     /database="snapgene"
                     /identity="99.8"
                     /match_length="100.0"
                     /fragment="False"
                     /other="rep_origin"
     terminator      complement(3842..3869)
                     /note="pLannotate"
                     /label="T7Te terminator"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="terminator"
     oriT            complement(2544..2652)
                     /note="pLannotate"
                     /label="oriT"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="99.1"
                     /fragment="False"
                     /other="oriT"
     CDS             1002..1283
                     /note="pLannotate"
                     /label="tnpA (fragment)"
                     /database="swissprot"
                     /identity="95.7"
                     /match_length="19.7"
                     /fragment="True"
                     /other="CDS"
     rep_origin      321..355
                     /note="pLannotate"
                     /label="CloDF13 ori (fragment)"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="4.7"
                     /fragment="True"
                     /other="rep_origin"
ORIGIN
        1 ggcagaggtc cgcccttggt gacagtgagc tttaccgtct gttccccctc gtagggctta
       61 ccttttccat cgccttcgac ctcaaagtag tgtccattga ccgtgcctga catataaacc
      121 ttgtaggtca tttgtttagc gatcacactc atatgtaata tctcctctgt ttctgtataa
      181 cagtactatg tgattatacc agccccctca ctacatgtca agaataaact gccaaagctg
      241 gtctcaaggg gcctgtcaga ccaagtttac tcatatatac tttagattga tttcagacag
      301 ctgggcgcgc cgtagaaaaa atcaaaggat cttcttgaga tccttttttt ctgcggggga
      361 tcaggaccgc tgccggagcg caacccactc actacagcag agccatgtag ggccgccggc
      421 gttgtggata cctcgcggaa aacttggccc tcactgacag atgaggggcg gacgttgaca
      481 cttgaggggc cgactcaccc ggcgcggcgt tgacagatga ggggcaggct cgatttcggc
      541 cggcgacgtg gagctggcca gcctcgcaaa tcggcgaaaa cgcctgattt tacgcgagtt
      601 tcccacggat gatgtggaca agcctgggga taagtgccct gcggtattga cacttgaggg
      661 gcgcgactac tgacagatga ggggcgcgat ccttgacact tgaggggcag agtgctgaca
      721 gatgaggggc gcacctattg acatttgagg ggctgtccac aggcagaaaa tccagcattt
      781 gcaagggttt ccgcccgttt ttcggccacc gctaacctgt cttttaacct gcttttaaac
      841 caatatttat aaaccttgtt tttaaccagg gctgcgccct gtgcgcgtga ccgcgcacgc
      901 cgaagggggg tgccccccct tctcgaaccc tcccggcccg ctaacgcggg cctcccatcc
      961 ccccaggggc tgcgcccctc ggccgcgaac ggcctcaccc caaaaatggc agccacgtag
     1021 aaagccagtc cgcagaaacg gtgctgaccc cggatgaatg tcagctactg ggctatctgg
     1081 acaagggaaa acgcaagcgc aaagagaaag caggtagctt gcagtgggct tacatggcga
     1141 tagctagact gggcggtttt atggacagca agcgaaccgg aattgccagc tggggcgccc
     1201 tctggtaagg ttgggaagcc ctgcaaagta aactggatgg ctttcttgcc gccaaggatc
     1261 tgatggcgca ggggatcaag atcgacggat cgatccgggg aattaattcc ggggcaatcc
     1321 cgcaaggagg gtgaatgaat cggacgtttg accggaaggc atacaggcaa gaactgatcg
     1381 acgcggggtt ttccgccgag gatgccgaaa ccatcgcaag ccgcaccgtc atgcgtgcgc
     1441 cccgcgaaac cttccagtcc gtcggctcga tagtccagca agctacggcc aagatcgagc
     1501 gcgacagcgt gcaactggct ccccctgccc tgcccgcgcc atcggccgcc gtggagcgtt
     1561 cgcgtcgtct ggaacaggag gcggcaggtt tggcgaagtc gatgaccatc gacacgcgag
     1621 gaactatgac gaccaagaag cgaaaaaccg ccggcgagga cctggcaaaa caggtcagcg
     1681 aagccaagca ggccgcgttg ctgaaacaca cgaagcagca gatcaaggaa atgcagcttt
     1741 ccttgttcga tattgcgccg tggccggaca cgatgcgagc gatgccaaac gacacggccc
     1801 gctctgccct gttcaccacg cgcaacaaga aaatcccgcg cgaggcgctg caaaacaagg
     1861 tcattttcca cgtcaacaag gacgtgaaga tcacctacac cggcgtcgag ctgcgggccg
     1921 acgatgacga actggtgtgg cagcaggtgt tggagtacgc gaagcgcacc cctatcggcg
     1981 agccgatcac cttcacgttc tacgagcttt gccaggacct gggctggtcg atcaatggcc
     2041 ggtattacac gaaggccgag gaatgcctgt cgcgcctaca ggcgacggcg atgggcttca
     2101 cgtccgaccg cgttgggcac ctggaatcgg tgtcgctgct gcaccgcttc cgcgtcctgg
     2161 accgtggcaa gaaaacgtcc cgttgccagg tcctgatcga cgaggaaatc gtcgtgctgt
     2221 ttgctggcga ccactacacg aaattcatat gggagaagta ccgcaagctg tcgccgacgg
     2281 cccgacggat gttcgactat ttcagctcgc accgggagcc gtacccgctc aagctggaaa
     2341 ccttccgcct catgtgcgga tcggattcca cccgcgtgaa gaagtggcgc gagcaggtcg
     2401 gcgaagcctg cgaagagttg cgaggcagcg gcctggtgga acacgcctgg gtcaatgatg
     2461 acctggtgca ttgcaaacgc tagggccttg tggggtcagt tccggctggg ggttcagcag
     2521 ccacctgcat cgcggccggc ctacggccag cctcgcagag caggattccc gttgagcacc
     2581 gccaggtgcg aataagggac agtgaagaag gaacacccgc tcgcgggtgg gcctacttca
     2641 cctatcctgc ccggctgacg ccgttggata caccaaggaa agtctacacg aaccctttgg
     2701 caaaatcctg tatatcgtgc gaaaaaggat ggatataccg aaaaaatcgc tataatgacc
     2761 ccgaagcagg gttatgcagc aaggcggcag gctgactcac gttaagggat tttggtcatg
     2821 actgatcctt caactcagca aaagttcgat ttattcaaca aagccacgtt gtgtctcaaa
     2881 atctctgatg ttacattgca caagataaaa atatatcatc atgaacaata aaactgtctg
     2941 cttacataaa cagtaataca aggggtgtta tgagccatat tcaacgggaa acgtcttgct
     3001 ccaggccgcg attaaattcc aacatggatg ctgatttata tgggtataaa tgggctcgcg
     3061 ataatgtcgg gcaatcaggt gcgacaatct atcgattgta tgggaagccc gatgcgccag
     3121 agttgtttct gaaacatggc aaaggtagcg ttgccaatga tgttacagat gagatggtca
     3181 gactaaactg gctgacggaa tttatgcctc ttccgaccat caagcatttt atccgtactc
     3241 ctgatgatgc atggttactc accactgcga tccccgggaa aacagcattc caggtattag
     3301 aagaatatcc tgattcaggt gaaaatattg ttgatgcgct ggcagtgttc ctgcgccggt
     3361 tgcattcgat tcctgtttgt aattgtcctt ttaacagcga tcgcgtattt cgcctcgctc
     3421 aggcgcaatc acgaatgaat aacggtttgg ttgatgcgag tgattttgat gacgagcgta
     3481 atggctggcc tgttgaacaa gtctggaaag aaatgcataa gcttttgcca ttctcaccgg
     3541 attcagtcgt cactcatggt gatttctcac ttgataacct tatttttgac gaggggaaat
     3601 taataggttg tattgatgtt ggacgagtcg gaatcgcaga ccgataccag gatcttgcca
     3661 tcctatggaa ctgcctcggt gagttttctc cttcattaca gaaacggctt tttcaaaaat
     3721 atggtattga taatcctgat atgaataaat tgcagtttca tttgatgctc gatgagtttt
     3781 tctaatcaga attggttaat tggttgtaac acgcggccgc tcggtgagac cgacttataa
     3841 acgcagaaag gcccacccga aggtgagcca gtgtaactct agtagagagc gttcaccgac
     3901 aaacaacaga taaaacgaaa ggcccagtct ttcgactgag cctttcgttt tatttgatgc
     3961 ctggagatcc ttttattagg cgaccacagg tttgcgtgca atggaaattt cacactgctc
     4021 aaccgaagtg taatccttgt tgtgattggt tacatccagt ttgcggtcaa catagtgata
     4081 ccctggcatc ttcacaggct tctttgcctt gtaagtagtt ttaaattcac acaaatagtg
     4141 accgcctcct tctaacttca gagccataaa gttgtttcct agcagcattc catctcgtgc
     4201 aaagagacgc tcagtgttgg gttcccagcc ctgtgtcttc ttctgcatga caggtccatt
     4261 gggaggaaag ttcaaaccag agaacttgac atggtagatg aaacagttgc cttggatgct
     4321 ggaatcattg ctgacagtac acactgcacc atcttcaaag ttcatgatcc tctcccatgt
     4381 atagccctcc gggaatgact gctttacata gtcagggatg tctccagggt acttggtgaa
     4441 tggtatgctt ccgtactgac actgtggtga taaaatatcc caagcaaat
//
