LOCUS       pLenti6.3-IGFBP2        8689 bp DNA     circular SYN 26-AUG-2022
DEFINITION  .
ACCESSION   .
VERSION     .
KEYWORDS    pLenti6.3/TO/V5-DEST-IGFBP2
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8689)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Aug 26, 2022 from SnapGene 6.1.0
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..8689
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..229
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
                     /note="color: #ffffff; direction: RIGHT"
     LTR             230..410
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
                     /note="color: #ffe4c4"
     misc_feature    457..582
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
                     /note="color: #ffe4c4"
     misc_feature    1075..1308
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
                     /note="color: #ffe4c4"
     misc_feature    1804..1921
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
                     /note="color: #ffe4c4"
     promoter        2247..2450
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
                     /note="color: #ffffff; direction: RIGHT"
     TATA_signal     2436..2442
                     /note="color: #a6acb3"
     protein_bind    2452..2470
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
                     /note="color: #31849b"
     protein_bind    2473..2491
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
                     /note="color: #31849b"
     protein_bind    2532..2556
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
                     /note="color: #31849b; direction: RIGHT"
     CDS             2562..3548
                     /codon_start=1
                     /label=IGFBP2
                     /note="Synthetic construct clone IMAGE:100006080; 
                     FLH191662.01X; RZPDo839F0877D insulin-like growth factor 
                     binding protein 2, 36kDa (IGFBP2) gene, encodes complete 
                     protein 
                     GenBank: DQ893450.2 
                     >DQ893450.2 Synthetic construct clone IMAGE:100006080; 
                     FLH191662.01X; RZPDo839F0877D insulin-like growth factor 
                     binding protein 2, 36kDa (IGFBP2) gene, encodes complete 
                     protein"
                     /note="color: #ff9900"
                     /translation="MLPRVGCPALPLPPPPLLPLLPLLLLLLGASGGGGGARAEVLFRC
                     PPCTPERLAACGPPPVAPPAAVAAVAGGARMPCAELVREPGCGCCSVCARLEGEACGVY
                     TPRCGQGLRCYPHPGSELPLQALVMGEGTCEKRRDAEYGASPEQVADNGDDHSEGGLVE
                     NHVDSTMNMLGGGGSAGRKPLKSGMKELAVFREKVTEQHRQMGKGGKHHLGLEEPKKLR
                     PPPARTPCQQELDQVLERISTMRLPDERGPLEHLYSLHIPNCDKHGLYNLKQCKMSLNG
                     QRGECWCVNPNTGKLIQGAPTIRGDPECHLFYNEQQEARGVHTQRMQ"
     CDS             2565..2678
                     /codon_start=1
                     /label=Signal peptide
                     /note="color: #00ccff"
                     /translation="LPRVGCPALPLPPPPLLPLLPLLLLLLGASGGGGGARA"
     CDS             3213..3233
                     /codon_start=1
                     /label=cNLS
                     /note="color: #993366"
                     /translation="PKKLRPP"
     protein_bind    complement(3550..3574)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
                     /note="color: #31849b; direction: LEFT"
     CDS             3627..3668
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /note="color: #cc99b2"
                     /translation="GKPIPNPLLGLDST"
     misc_feature    3692..4280
                     /label=WPRE
                     /note="woodchuck posttranscriptional regulatory element"
                     /note="color: #ffffff"
     promoter        4295..4611
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
                     /note="color: #ffffff; direction: RIGHT"
     rep_origin      4462..4597
                     /label=SV40 ori
                     /note="SV40 origin of replication"
                     /note="color: #ffff00"
     promoter        4659..4706
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
                     /note="color: #ffffff; direction: RIGHT"
     CDS             4725..5123
                     /codon_start=1
                     /gene="Aspergillus terreus BSD"
                     /product="blasticidin S deaminase"
                     /label=BSD
                     /note="confers resistance to blasticidin"
                     /note="color: #ccffcc"
                     /translation="MAKPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFTG
                     VNVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHPGI
                     KAIVKDSDGQPTAVGIRELLPSGYVWEG"
     LTR             5210..5443
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
                     /note="color: #ffe4c4"
     polyA_signal    5515..5636
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
                     /note="color: #a6acb3"
     rep_origin      6018..6473
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
                     /note="color: #ffff00"
     promoter        6499..6603
                     /gene="bla"
                     /label=AmpR promoter
                     /note="color: #ffffff; direction: RIGHT"
     CDS             6604..7464
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /note="This feature has 2 segments:
                        1: 6604 .. 6672 / #ccffcc / signal sequence
                        2: 6673 .. 7464 / #ccffcc
                     Cleavage site after base 6672"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      7635..8223
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
                     /note="color: #ffff00"
ORIGIN
        1 aatgtagtct tatgcaatac tcttgtagtc ttgcaacatg gtaacgatga gttagcaaca
       61 tgccttacaa ggagagaaaa agcaccgtgc atgccgattg gtggaagtaa ggtggtacga
      121 tcgtgcctta ttaggaaggc aacagacggg tctgacatgg attggacgaa ccactgaatt
      181 gccgcattgc agagatattg tatttaagtg cctagctcga tacataaacg ggtctctctg
      241 gttagaccag atctgagcct gggagctctc tggctaacta gggaacccac tgcttaagcc
      301 tcaataaagc ttgccttgag tgcttcaagt agtgtgtgcc cgtctgttgt gtgactctgg
      361 taactagaga tccctcagac ccttttagtc agtgtggaaa atctctagca gtggcgcccg
      421 aacagggact tgaaagcgaa agggaaacca gaggagctct ctcgacgcag gactcggctt
      481 gctgaagcgc gcacggcaag aggcgagggg cggcgactgg tgagtacgcc aaaaattttg
      541 actagcggag gctagaagga gagagatggg tgcgagagcg tcagtattaa gcgggggaga
      601 attagatcgc gatgggaaaa aattcggtta aggccagggg gaaagaaaaa atataaatta
      661 aaacatatag tatgggcaag cagggagcta gaacgattcg cagttaatcc tggcctgtta
      721 gaaacatcag aaggctgtag acaaatactg ggacagctac aaccatccct tcagacagga
      781 tcagaagaac ttagatcatt atataataca gtagcaaccc tctattgtgt gcatcaaagg
      841 atagagataa aagacaccaa ggaagcttta gacaagatag aggaagagca aaacaaaagt
      901 aagaccaccg cacagcaagc ggccgctgat cttcagacct ggaggaggag atatgaggga
      961 caattggaga agtgaattat ataaatataa agtagtaaaa attgaaccat taggagtagc
     1021 acccaccaag gcaaagagaa gagtggtgca gagagaaaaa agagcagtgg gaataggagc
     1081 tttgttcctt gggttcttgg gagcagcagg aagcactatg ggcgcagcgt caatgacgct
     1141 gacggtacag gccagacaat tattgtctgg tatagtgcag cagcagaaca atttgctgag
     1201 ggctattgag gcgcaacagc atctgttgca actcacagtc tggggcatca agcagctcca
     1261 ggcaagaatc ctggctgtgg aaagatacct aaaggatcaa cagctcctgg ggatttgggg
     1321 ttgctctgga aaactcattt gcaccactgc tgtgccttgg aatgctagtt ggagtaataa
     1381 atctctggaa cagatttgga atcacacgac ctggatggag tgggacagag aaattaacaa
     1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga
     1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa
     1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat
     1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt
     1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg
     1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtatcggtt
     1801 aacttttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat
     1861 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaaa ttcaaaattt
     1921 tatcgataag cttgggagtt ccgcttacat aacttacggt aaatggcccg cctggctgac
     1981 cgcccaacga cccccgccca ttgacgtcaa taatgacgta tgttcccata gtaacgccaa
     2041 tagggacttt ccattgacgt caatgggtgg agtatttacg gtaaactgcc cacttggcag
     2101 tacatcaagt gtatcatatg ccaagtacgc cccctattga cgtcaatgac ggtaaatggc
     2161 ccgcctggca ttatgcccag tacatgacct tatgggactt tcctacttgg cagtacatct
     2221 acgtattagt catcgctatt accatggtga tgcggttttg gcagtacatc aatgggcgtg
     2281 gatagcggtt tgactcacgg ggatttccaa gtctccaccc cattgacgtc aatgggagtt
     2341 tgttttggca ccaaaatcaa cgggactttc caaaatgtcg taacaactcc gccccattga
     2401 cgcaaatggg cggtaggcgt gtacggtggg aggtctatat aagcagagct ctccctatca
     2461 gtgatagaga tctccctatc agtgatagag atcgtcgact agtccagtgt ggtggaattc
     2521 tgcagatatc aacaagtttg tacaaaaaag caggctccac catgctgccg agagtgggct
     2581 gccccgcgct gccgctgccg ccgccgccgc tgctgccgct gctgccgctg ctgctgctgc
     2641 tactgggcgc gagtggcggc ggcggcgggg cgcgcgcgga ggtgctgttc cgctgcccgc
     2701 cctgcacacc cgagcgcctg gccgcctgcg ggcccccgcc ggttgcgccg cccgccgcgg
     2761 tggccgcagt ggccggaggc gcccgcatgc catgcgcgga gctcgtccgg gagccgggct
     2821 gcggctgctg ctcggtgtgc gcccggctgg agggcgaggc gtgcggcgtc tacaccccgc
     2881 gctgcggcca ggggctgcgc tgctatcccc acccgggctc cgagctgccc ctgcaggcgc
     2941 tggtcatggg cgagggcact tgtgagaagc gccgggacgc cgagtatggc gccagcccgg
     3001 agcaggttgc agacaatggc gatgaccact cagaaggagg cctggtggag aaccacgtgg
     3061 acagcaccat gaacatgttg ggcgggggag gcagtgctgg ccggaagccc ctcaagtcgg
     3121 gtatgaagga gctggccgtg ttccgggaga aggtcactga gcagcaccgg cagatgggca
     3181 agggtggcaa gcatcacctt ggcctggagg agcccaagaa gctgcgacca ccccctgcca
     3241 ggactccctg ccaacaggaa ctggaccagg tcctggagcg gatctccacc atgcgccttc
     3301 cggatgagcg gggccctctg gagcacctct actccctgca catccccaac tgtgacaagc
     3361 atggcctgta caacctcaaa cagtgcaaga tgtctctgaa cgggcagcgt ggggagtgct
     3421 ggtgtgtgaa ccccaacacc gggaagctga tccagggagc ccccaccatc cggggggacc
     3481 ccgagtgtca tctcttctac aatgagcagc aggaggctcg cggggtgcac acccagcgga
     3541 tgcagtagga cccagctttc ttgtacaaag tggttgatat ccagcacagt ggcggccgct
     3601 cgagtctaga gggcccgcgg ttcgaaggta agcctatccc taaccctctc ctcggtctcg
     3661 attctacgcg taccggttag taatgatcga caatcaacct ctggattaca aaatttgtga
     3721 aagattgact ggtattctta actatgttgc tccttttacg ctatgtggat acgctgcttt
     3781 aatgcctttg tatcatgcta ttgcttcccg tatggctttc attttctcct ccttgtataa
     3841 atcctggttg ctgtctcttt atgaggagtt gtggcccgtt gtcaggcaac gtggcgtggt
     3901 gtgcactgtg tttgctgacg caacccccac tggttggggc attgccacca cctgtcagct
     3961 cctttccggg actttcgctt tccccctccc tattgccacg gcggaactca tcgccgcctg
     4021 ccttgcccgc tgctggacag gggctcggct gttgggcact gacaattccg tggtgttgtc
     4081 ggggaagctg acgtcctttc catggctgct cgcctgtgtt gccacctgga ttctgcgcgg
     4141 gacgtccttc tgctacgtcc cttcggccct caatccagcg gaccttcctt cccgcggcct
     4201 gctgccggct ctgcggcctc ttccgcgtct tcgccttcgc cctcagacga gtcggatctc
     4261 cctttgggcc gcctccccgc ctggcgatgg taccggtgtg gaaagtcccc aggctcccca
     4321 gcaggcagaa gtatgcaaag catgcatctc aattagtcag caaccaggtg tggaaagtcc
     4381 ccaggctccc cagcaggcag aagtatgcaa agcatgcatc tcaattagtc agcaaccata
     4441 gtcccgcccc taactccgcc catcccgccc ctaactccgc ccagttccgc ccattctccg
     4501 ccccatggct gactaatttt ttttatttat gcagaggccg aggccgcctc tgcctctgag
     4561 ctattccaga agtagtgagg aggctttttt ggaggcctag gcttttgcaa aaagctcccg
     4621 ggagcttgta tatccatttt cggatctgat cagcacgtgt tgacaattaa tcatcggcat
     4681 agtatatcgg catagtataa tacgacaagg tgaggaacta aaccatggcc aagcctttgt
     4741 ctcaagaaga atccaccctc attgaaagag caacggctac aatcaacagc atccccatct
     4801 ctgaagacta cagcgtcgcc agcgcagctc tctctagcga cggccgcatc ttcactggtg
     4861 tcaatgtata tcattttact gggggacctt gtgcagaact cgtggtgctg ggcactgctg
     4921 ctgctgcggc agctggcaac ctgacttgta tcgtcgcgat cggaaatgag aacaggggca
     4981 tcttgagccc ctgcggacgg tgccgacagg tgcttctcga tctgcatcct gggatcaaag
     5041 ccatagtgaa ggacagtgat ggacagccga cggcagttgg gattcgtgaa ttgctgccct
     5101 ctggttatgt gtgggagggc taagcacaat tcgagctcgg tacctttaag accaatgact
     5161 tacaaggcag ctgtagatct tagccacttt ttaaaagaaa aggggggact ggaagggcta
     5221 attcactccc aacgaagaca agatctgctt tttgcttgta ctgggtctct ctggttagac
     5281 cagatctgag cctgggagct ctctggctaa ctagggaacc cactgcttaa gcctcaataa
     5341 agcttgcctt gagtgcttca agtagtgtgt gcccgtctgt tgtgtgactc tggtaactag
     5401 agatccctca gaccctttta gtcagtgtgg aaaatctcta gcagtagtag ttcatgtcat
     5461 cttattattc agtatttata acttgcaaag aaatgaatat cagagagtga gaggaacttg
     5521 tttattgcag cttataatgg ttacaaataa agcaatagca tcacaaattt cacaaataaa
     5581 gcattttttt cactgcattc tagttgtggt ttgtccaaac tcatcaatgt atcttatcat
     5641 gtctggctct agctatcccg cccctaactc cgcccatccc gcccctaact ccgcccagtt
     5701 ccgcccattc tccgccccat ggctgactaa ttttttttat ttatgcagag gccgaggccg
     5761 cctcggcctc tgagctattc cagaagtagt gaggaggctt ttttggaggc ctagggacgt
     5821 acccaattcg ccctatagtg agtcgtatta cgcgcgctca ctggccgtcg ttttacaacg
     5881 tcgtgactgg gaaaaccctg gcgttaccca acttaatcgc cttgcagcac atcccccttt
     5941 cgccagctgg cgtaatagcg aagaggcccg caccgatcgc ccttcccaac agttgcgcag
     6001 cctgaatggc gaatgggacg cgccctgtag cggcgcatta agcgcggcgg gtgtggtggt
     6061 tacgcgcagc gtgaccgcta cacttgccag cgccctagcg cccgctcctt tcgctttctt
     6121 cccttccttt ctcgccacgt tcgccggctt tccccgtcaa gctctaaatc gggggctccc
     6181 tttagggttc cgatttagtg ctttacggca cctcgacccc aaaaaacttg attagggtga
     6241 tggttcacgt agtgggccat cgccctgata gacggttttt cgccctttga cgttggagtc
     6301 cacgttcttt aatagtggac tcttgttcca aactggaaca acactcaacc ctatctcggt
     6361 ctattctttt gatttataag ggattttgcc gatttcggcc tattggttaa aaaatgagct
     6421 gatttaacaa aaatttaacg cgaattttaa caaaatatta acgcttacaa tttaggtggc
     6481 acttttcggg gaaatgtgcg cggaacccct atttgtttat ttttctaaat acattcaaat
     6541 atgtatccgc tcatgagaca ataaccctga taaatgcttc aataatattg aaaaaggaag
     6601 agtatgagta ttcaacattt ccgtgtcgcc cttattccct tttttgcggc attttgcctt
     6661 cctgtttttg ctcacccaga aacgctggtg aaagtaaaag atgctgaaga tcagttgggt
     6721 gcacgagtgg gttacatcga actggatctc aacagcggta agatccttga gagttttcgc
     6781 cccgaagaac gttttccaat gatgagcact tttaaagttc tgctatgtgg cgcggtatta
     6841 tcccgtattg acgccgggca agagcaactc ggtcgccgca tacactattc tcagaatgac
     6901 ttggttgagt actcaccagt cacagaaaag catcttacgg atggcatgac agtaagagaa
     6961 ttatgcagtg ctgccataac catgagtgat aacactgcgg ccaacttact tctgacaacg
     7021 atcggaggac cgaaggagct aaccgctttt ttgcacaaca tgggggatca tgtaactcgc
     7081 cttgatcgtt gggaaccgga gctgaatgaa gccataccaa acgacgagcg tgacaccacg
     7141 atgcctgtag caatggcaac aacgttgcgc aaactattaa ctggcgaact acttactcta
     7201 gcttcccggc aacaattaat agactggatg gaggcggata aagttgcagg accacttctg
     7261 cgctcggccc ttccggctgg ctggtttatt gctgataaat ctggagccgg tgagcgtggg
     7321 tctcgcggta tcattgcagc actggggcca gatggtaagc cctcccgtat cgtagttatc
     7381 tacacgacgg ggagtcaggc aactatggat gaacgaaata gacagatcgc tgagataggt
     7441 gcctcactga ttaagcattg gtaactgtca gaccaagttt actcatatat actttagatt
     7501 gatttaaaac ttcattttta atttaaaagg atctaggtga agatcctttt tgataatctc
     7561 atgaccaaaa tcccttaacg tgagttttcg ttccactgag cgtcagaccc cgtagaaaag
     7621 atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa
     7681 aaaccaccgc taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg
     7741 aaggtaactg gcttcagcag agcgcagata ccaaatactg ttcttctagt gtagccgtag
     7801 ttaggccacc acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg
     7861 ttaccagtgg ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga
     7921 tagttaccgg ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc
     7981 ttggagcgaa cgacctacac cgaactgaga tacctacagc gtgagctatg agaaagcgcc
     8041 acgcttcccg aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga
     8101 gagcgcacga gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt
     8161 cgccacctct gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg
     8221 aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct tttgctggcc ttttgctcac
     8281 atgttctttc ctgcgttatc ccctgattct gtggataacc gtattaccgc ctttgagtga
     8341 gctgataccg ctcgccgcag ccgaacgacc gagcgcagcg agtcagtgag cgaggaagcg
     8401 gaagagcgcc caatacgcaa accgcctctc cccgcgcgtt ggccgattca ttaatgcagc
     8461 tggcacgaca ggtttcccga ctggaaagcg ggcagtgagc gcaacgcaat taatgtgagt
     8521 tagctcactc attaggcacc ccaggcttta cactttatgc ttccggctcg tatgttgtgt
     8581 ggaattgtga gcggataaca atttcacaca ggaaacagct atgaccatga ttacgccaag
     8641 cgcgcaatta accctcacta aagggaacaa aagctggagc tgcaagctt
//
