LOCUS       45952_22                7838 bp DNA     circular SYN 29-OCT-2016
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7838)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Sep 7, 2022 from SnapGene 6.1.1
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..7838
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     source          join(4138..4147,4574..5428)
                     /mol_type="other DNA"
                     /label=source:pPD95_62
                     /organism="pPD95_62"
     primer_bind     6..22
                     /label=M13R_GW_PRIMER
     misc_feature    113..131
                     /label=EV Landmark FWD
     misc_feature    170..3089
                     /label=flp-19
     misc_feature    2902..2919
                     /label=EV flp-19 End FWD
     misc_feature    3194..3235
                     /label=1xNLS
     misc_feature    3195..3214
                     /label=Color FWD
     misc_feature    3236..3271
                     /label=NLS2
     misc_feature    join(3272..3445,3497..3679,3731..3922,3974..4123)
                     /label=ceBFP2
     misc_feature    3605..3622
                     /label=EV ceBFP2 Mid FWD
     misc_feature    3653..3672
                     /label=EV ceBFP2 Mid REV
     misc_feature    3760..3778
                     /label=EV ceBFP2 Mid2 FWD
     misc_feature    3886..3903
                     /label=EV ceBFP2 Mid2 REV
     misc_feature    3893..3895
                     /label=I to A mTagBFP2 Fix
     misc_feature    complement(4124..4129)
                     /label=Linker AvrII Cut Site
     misc_feature    complement(4130..4131)
                     /label=Frame Link
     misc_feature    complement(4132..4137)
                     /label=AgeI Cut Site
     misc_feature    4148..4513
                     /label=H2B
     misc_feature    4295..4314
                     /label=H2B Mid REV
     misc_feature    4514..4519
                     /label=EcoRI Cut Site
     misc_feature    4520..4573
                     /label=Ligated Addition
     misc_feature    4642..5369
                     /label=unc-54 3'UTR
     misc_feature    4652..4670
                     /label=EV unc-54 3'UTR REV
     misc_feature    5120..5272
                     /label=aex-5 Exon1
     primer_bind     complement(5370..5395)
                     /label=Primer D54
     primer_bind     complement(5390..5415)
                     /label=PrimerD858
     misc_feature    5407..5424
                     /label=EV Landmark REV
     primer_bind     complement(5429..5451)
                     /label=3GEX_GW_PRIMER
     misc_feature    5429..5446
                     /label=EV ceBFP2 + H2B GIB REV
     CDS             6037..6403
                     /label=AmpR
     CDS             6404..6696
                     /label=AmpR
                     /label=AmpR(1)
     rep_origin      6794..7476
                     /label=ColE1 origin
     misc_binding    7816..7838
                     /label=LacO
ORIGIN
        1 tcacacagga aacagctatg accatgatta cgccaagctg taagtttaaa catgatctta
       61 ctaactaact attctcattt aaattttcag agcttaaaaa tggctgaaat cactcacaac
      121 gatggatacg ctaacaactt ggaaatgaaa taagcttgca tgcctgcagt gactcaccgt
      181 agtaatccat atatccagac cccttaaaat tttgttcaac ggttatcttt tttttgtttt
      241 tacagagaat aatatgtttt cttgtagcat tgggatcttt caagtattat tcagcagtgt
      301 catgtgatat ttttctaccg aacgtggtta aattaataat tttttatggc ccctattttt
      361 tacgcaatgc tgtaagaatt ttttttgata atcttccaat aaccactgac cgcattctag
      421 ctgtgttctg ttgaacaaaa taaattttag cttttcgtat tataaacaga attctcacgg
      481 ctgttcagct gaaatacaga tttacggacg acttgaaaat ttgaagatcc ttttgaaaat
      541 ttttgttttt gaaaaaatcc caaacactta aaaaaaaacc aatcgcaatt tattttgaaa
      601 gaatcattaa aaatgcgtta cacaaacagt tctagtaaaa acacaatccc attacggtac
      661 taatcaaaaa taattttcta ataaaaatcg gattctgctc atattactct tcaaatattt
      721 gattttacaa acttaatata atggcaagag agcaattgta ttttttttcc acaagatcgc
      781 aatctagata tttgttataa caaaactaca tgaaagaatt atagtggtca cacgattgaa
      841 aaaaaatttt tgggtctagt taatttctca gaatcgagtt taacttcatg ggaacggagg
      901 ttttaaattt cccaaagatt acttccacaa caactcggct aatttcggaa ctattttttt
      961 gattcgcgta tgtttgcttg gttaattcca tcaatgacgt gtattttcat aaactcgaaa
     1021 taattgttac cttgtatgct ttaaactatg ttatgtattt tttgaatttg ctccatacat
     1081 tatttcaagt gaaaattgtt tataaaagtt ggctctagtc aaaacgtggt tttggaaaaa
     1141 aaacacattc actgtaatca aattgtactg aaatcaagaa atttgttttt tctcaaattt
     1201 tgtgtttttc aaatgctttt gcgtcatcaa atttgattat caaatcccga agtcagaact
     1261 ttagaaccta aaactaaaat cggaattgaa actgatgtca tcgtgggaaa aaaacggaaa
     1321 ccacatgtta tcacttattt atccctgatc gtttgctgtt aactgataga ataaatgaaa
     1381 gatgtttcaa cttaaaatat aataatattc cacgtaagca ctggatatat tttatatgta
     1441 actcatcaac aaacagtgtg atgtacaaat tattatcatc ataaaaacag ataaagtcga
     1501 ggtaaatgta aacgttttta aatacagacc aacaattcct ccgaaaaact gttcttattt
     1561 aactcaacct tgttcataaa taacttggat gtgcatcttg atttatgaca cttatacagt
     1621 gtattgtcta ttttcattgt tttagtttga tagatcggaa aacaagagct gagattgatt
     1681 atttgaaacg agatttatga ttcattagtc atgtataaca agttctgaca gaagattatg
     1741 actacaatgg tagtagagtt ttcccagccg agttctctat gattgagtac aatttaactt
     1801 tctcttctga tgaaaatgct ttcaatgacg tcaatagaaa aatcatagcg tcgccataga
     1861 agtgattttt tggctccatg gtagcccgcc tcaggaagtg agaatttaaa atttgaaatt
     1921 ctaatcgttt tcgaaaatgc tattctggaa aagaaacaca tgtatggtgt gttttaactt
     1981 tttagaataa aaaatttctg tggcacaaca cagaatttag cattaaaatt catgaagctc
     2041 tctgcgagat atgggttgaa aacagcaaca aattaaaatt taaatgaaat ctgtttaatt
     2101 ttttgctcct ttcctgcaag cgcaagcgtt gatgagactt ccgctcacaa atgaagtttt
     2161 atttttacag cgtctctatt gatatctaag cggattattt aaattaaaaa tagtgttaca
     2221 ctgtttttaa ttttcgcttt cagaagtatt aacaattttc aagttttatt aactacacct
     2281 tatcaaaccg ttttgtaaca actacttcat ttctttgaat tccctgcggt aacatagact
     2341 gaacacaaaa taatatagaa ttatcatggt acctgttcag tgactaactg agaaaaatgc
     2401 caaaaatcac caaatccgtt attaagcgtc tagaccgtat gtagaattgc taagaacaag
     2461 aacattgtac cgcatgaaat aatggaaagt aatttataac atctccaaaa ttcaattttt
     2521 tgaaattggc cagcaaaatt ggaacaacta tcataacaag ttttctcaaa cgttctttat
     2581 ttttaactaa gtcgagaact gaaacaaacg cattaaactt caatttttct tttcttgttc
     2641 ttttatatcc ggaaaaccac atttcagatc tcaaatctaa actaagagaa actaaaaaaa
     2701 agtctacctt gaattataaa gtaggtttga gaaagcaacc cacacctcaa ctttcgtgct
     2761 tccggatatc ttcaaatatc atcactttct tattttccgg gctcgaaata ctggaaaaac
     2821 ttctaggctg cacatcttaa aaagtagcgg tgacattcct cccattccca tccttctcac
     2881 ttttggttca ttagcactat attccatagt gctccaccca catttcaccg gtttgtcgcg
     2941 tcgtctctca ttcttactat agaaaactct tgaattcctt gtagatatcg cttcttagtc
     3001 tttgacaagc cacgtccaca aaacccgcga gtgctctcaa cgtgcctcaa ggaggacacg
     3061 cccaccactg cttcattcat tgcaccgacc cgggattggc caaaggaccc aaaggtatgt
     3121 ttcgaatgat actaacataa catagaacat tttcaggagg acccttgctt ggagggtacc
     3181 gagctcagaa aaaatgaccg ctccaaagaa gaagcgcaag gttccagtcg agaagatgac
     3241 tgccccaaaa aagaaacgta aggtcccgcg gatgtcagag cttattaagg agaatatgca
     3301 tatgaaactg tacatggaag gaaccgtgga caaccatcac tttaagtgca cttcggaggg
     3361 ggaaggcaaa ccgtacgagg gcacccagac catgcgaatc aaggtggtcg agggaggccc
     3421 actgcccttc gccttcgaca ttctggtaag tttaaacata tatatactaa ctaaccctga
     3481 ttatttaaat tttcaggcta ctagcttcct gtacggcagc aagactttta ttaatcacac
     3541 ccagggcatc ccggacttct tcaaacagtc gttccctgag ggcttcacat gggagcgtgt
     3601 gaccacatac gaagacggtg gcgttctcac cgctacccag gacaccagcc tccaggacgg
     3661 ttgcctcata tataatgtgg taagtttaaa cagttcggta ctaactaacc atacatattt
     3721 aaattttcag aagatccggg gagtaaactt cacatccaat ggtccggtta tgcagaagaa
     3781 aacactcggc tgggaggcct tcaccgagac gctgtatccg gctgacggag gcctggaagg
     3841 tcgaaacgac atggccctga agctcgtcgg cggtagccat ctcatcgcaa acgctaagac
     3901 cacatataga tccaagaaac cggtaagttt aaacatgatt ttactaacta actaatctga
     3961 tttaaatttt caggctaaga accttaaaat gccgggagtc tactatgttg actacagact
     4021 ggaacggatc aaggaggcca acaacgagac ctacgtcgag cagcacgagg ttgcagttgc
     4081 cagatactgc gacctcccga gcaaactggg tcacaagctt aatcctagga taccggtggg
     4141 tgaagaccca ccaaagccat ctgccaaggg agccaagaag gccgccaaga ccgttacgaa
     4201 gccaaaggac ggaaagaaga gacgtcatgc ccgtaaggaa tcatactccg tctacatcta
     4261 ccgtgtcctc aagcaagttc atccagacac tggagtttcc tccaaagcca tgtctatcat
     4321 gaactctttt gtcaacgatg tcttcgagcg tattgctgct gaagcatccc gtcttgctca
     4381 ctacaacaag cgttccacaa tctcatcccg cgaaattcag accgctgtcc gtctgatcct
     4441 tccaggagag cttgccaagc acgccgtgtc tgagggaacc aaggccgtta ccaagtacac
     4501 ttccagcaag taggaattcc aactgagcgc cggtcgctac cattaccaac ttgtctggtg
     4561 tcaaaaataa taggggccgc tgtcatcaga gtaagtttaa actgagttct actaactaac
     4621 gagtaatatt taaattttca gcatctcgcg cccgtgcctc tgacttctaa gtccaattac
     4681 tcttcaacat ccctacatgc tctttctccc tgtgctccca ccccctattt ttgttattat
     4741 caaaaaactt ctcttaattt ctttgttttt tagcttcttt taagtcacct ctaacaatga
     4801 aattgtgtag attcaaaaat agaattaatt cgtaataaaa agtcgaaaaa aattgtgctc
     4861 cctcccccca ttaataataa ttctatccca aaatctacac aatgttctgt gtacacttct
     4921 tatgtttttt acttctgata aatttttttg aaacatcata gaaaaaaccg cacacaaaat
     4981 accttatcat atgttacgtt tcagtttatg accgcaattt ttatttcttc gcacgtctgg
     5041 gcctctcatg acgtcaaatc atgctcatcg tgaaaaagtt ttggagtatt tttggaattt
     5101 ttcaatcaag tgaaagttta tgaaattaat tttcctgctt ttgctttttg gggtttcccc
     5161 tattgtttgt caagatttcg aggacggcgt ttttcttgct aaaatcacaa gtattgatga
     5221 gcacgatgca agaaagatcg gaagaaggtt tgggtttgag gctcagtgga aggtgagtag
     5281 aagttgataa tttgaaagtg gagtagtgtc tatggggttt ttgccttaaa tgacagaata
     5341 cattcccaat ataccaaaca taactgtttc ctactagtcg gccgtacggg ccctttcgtc
     5401 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca
     5461 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg
     5521 ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc
     5581 accatatgcg gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc atcaggcggc
     5641 cttaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct
     5701 tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc
     5761 taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa
     5821 tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt
     5881 gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct
     5941 gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc
     6001 cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta
     6061 tgtggcgcgg tattatcccg tattgacgcc gggcaagagc aactcggtcg ccgcatacac
     6121 tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc
     6181 atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac
     6241 ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg
     6301 gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac
     6361 gagcgtgaca ccacgatgcc tgtagcaatg gcaacaacgt tgcgcaaact attaactggc
     6421 gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt
     6481 gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga
     6541 gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc
     6601 cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag
     6661 atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca
     6721 tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc
     6781 ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca
     6841 gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc
     6901 tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta
     6961 ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt
     7021 ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc
     7081 gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg
     7141 ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg
     7201 tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag
     7261 cattgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc
     7321 agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat
     7381 agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg
     7441 gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc
     7501 tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt
     7561 accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca
     7621 gtgagcgagg aagcggaaga gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg
     7681 attcattaat gcagctggca cgacaggttt cccgactgga aagcgggcag tgagcgcaac
     7741 gcaattaatg tgagttagct cactcattag gcaccccagg ctttacactt tatgcttccg
     7801 gctcgtatgt tgtgtggaat tgtgagcgga taacaatt
//
