LOCUS       45952_38                7220 bp DNA     circular SYN 05-JUL-2016
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7220)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Sep 7, 2022 from SnapGene 6.1.1
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..7220
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     source          3956..4810
                     /mol_type="other DNA"
                     /label=source:pPD95_62
                     /organism="pPD95_62"
     primer_bind     6..22
                     /label=M13R_GW_PRIMER
     misc_feature    113..131
                     /label=EV Landmark FWD
     misc_feature    163
                     /label=SphI Cut Site
     misc_feature    164..169
                     /label=ApaI Cut Site 2
     misc_feature    170..2465
                     /label=flp-13
     misc_feature    170..190
                     /label=Kim & Li 2004 FWD
     misc_feature    2266..2287
                     /label=EV flp-13 End FWD
     misc_feature    2465
                     /label=SmaI Cut Site
     misc_feature    2570..2611
                     /label=1xNLS
     misc_feature    2571..2590
                     /label=Color FWD
     misc_feature    2612..2647
                     /label=NLS2
     misc_feature    2642..2647
                     /label=SacII Cut Site
     misc_feature    2698..2703
                     /label=BssHII Cut Site
     misc_feature    join(2705..2914,2966..3244,3296..3505)
                     /label=CyOFP1
     misc_feature    3080..3098
                     /label=EV CyOFP1 Mid FWD
     misc_feature    3132..3149
                     /label=EV CyOFP1 Mid REV
     misc_feature    3325..3343
                     /label=EV CyOFP Mid2 REV
     misc_feature    3506..3511
                     /label=AvrII Cut Site
     misc_feature    complement(3512..3513)
                     /label=Frame Link
     misc_feature    complement(3514..3519)
                     /label=AgeI Cut Site
     misc_feature    3530..3895
                     /label=H2B
     misc_feature    3677..3696
                     /label=H2B Mid REV
     misc_feature    3896..3901
                     /label=EcoRI Cut Site
     misc_feature    3902..3955
                     /label=Ligated Addition
     misc_feature    4024..4751
                     /label=unc-54 3'UTR
     misc_feature    4034..4052
                     /label=EV unc-54 3'UTR REV
     misc_feature    4502..4654
                     /label=aex-5 Exon1
     primer_bind     complement(4752..4777)
                     /label=Primer D54
     primer_bind     complement(4772..4797)
                     /label=PrimerD858
     misc_feature    4789..4806
                     /label=EV Landmark REV
     primer_bind     complement(4811..4833)
                     /label=3GEX_GW_PRIMER
     misc_feature    4811..4828
                     /label=EV ceBFP2 + H2B GIB REV
     CDS             5419..5785
                     /label=AmpR
     CDS             5786..6078
                     /label=AmpR
                     /label=AmpR(1)
     rep_origin      6176..6858
                     /label=ColE1 origin
     misc_binding    7198..7220
                     /label=LacO
ORIGIN
        1 tcacacagga aacagctatg accatgatta cgccaagctg taagtttaaa catgatctta
       61 ctaactaact attctcattt aaattttcag agcttaaaaa tggctgaaat cactcacaac
      121 gatggatacg ctaacaactt ggaaatgaaa taagcttgca tgcgggccca aattgtgcct
      181 cctgatgctg aatcctcaat ccatttctcc atacttcctt ttcacttcct ccatttggcg
      241 gaaggtgggc ggtcctcccg ccgaaacttg atctcttttt tatttttaag aagatccttt
      301 atgtttttta tattgacgtt tacttttttt ttggaagaaa gtgggtgaac tacaatcaga
      361 ttatttttat gctccgcgaa aatttaatca tgatcaaatg tgatatatga tgataatttg
      421 ggaataaaaa agtggaggtg gaacataatt tgtgttctta cggactataa tttatttgat
      481 ataaaacatc attatttcac ttgttgcagt caaaatgttc agacttgcaa ccgctttttt
      541 ctttaacccc atttaaatag gtcattttga aactgaaatt ttagatatca atgtaacaat
      601 tttcagaaaa tcatgaaaat gagaaaataa cactgaatct caattttttc taactagccg
      661 tttattaatt aaaactgtag tagcatttta gtcggcattt tgtgtcaaaa caaattcatt
      721 cttgtatttt tttttaatgc agtcaatatc ttagtaaatt gccaaatata cgaaacctat
      781 ggagcgtttc tgaggaattc agaacactag aacactgttc aatccttgct acatttaaat
      841 acagggtggg ccaaaagtat ggtaacacgt ttgcagctct ataactttac tagtggagag
      901 agttattaca tcaatttgta caaggtttgt tctccataaa atactgacca gatgaacgtt
      961 ttgaattttc aaaatattcc catccgcatc gctgcaggcc ttcagaactt ttgagatcac
     1021 gacctttatg gcacgcaagt cggcattctg cgggatttct acatttttct caaggaatcc
     1081 atcaagtgtc ttacaagttt ttatagttca aagaatgctc ttggtctaga gattttttaa
     1141 aaacaaaaat ccaaataatt tctatcacgg tatttctgtc atggactgcg gcagtaggtt
     1201 aagtcttgaa tggcatagtc gccagaaaga tggatgtgag cggcgagtag cagtatgcag
     1261 aaaaattcat tttagtattt ttcgagacgt tttgacctga ctttccagaa tttttttaga
     1321 aaattgagct cgaatgattt tgtgggtcgt tacgccttgc gaaaccaaaa aaactgagta
     1381 aatttcgcaa agttgaattc atgaaaaatt caaaggttct ggcgccagtg tcgacacaga
     1441 aaatttttgt atgctacaaa aacaatcaaa aacgcttctt cacttcttta actttaaatt
     1501 taacataaaa atcatacatc tgaaaattat tggcgtgcgt gggaggtgtg tttgtgtggg
     1561 aagtgccgcg tgccaaatta ccgttttttc gaaagggttc gccaaatttt cgaggtgata
     1621 aagatcgcac cttattggga aacattacaa attttgagtc aaattgaagg aaatttattc
     1681 agctatcagt gtttgataga agaatgtgcg tatgacgtgg cagtcgcgcg aaaagctcat
     1741 gtaaaagaac gcttcatgtg ttaccatact tttggcccac cctgtataaa tcgtgaaaac
     1801 ataattagat tataaaagta aaagtcgccc ggtttcgatt atggttggca aaatctgagt
     1861 aatcgctact tttttttgat tgcaattaaa aataaattct gacacctttt aaaaacatgt
     1921 accatgatat tttttaaacg atttcgcgac ttggactaca tcttttgaac tccaatatta
     1981 tcagttccga agtgtgatgt tgatgttaac ataatgacat ttacaacgta ttctgatttc
     2041 tttgaatatc ttctcgaact ctcgagacac atctgttcag attccagttg acttgtcaca
     2101 tttacgcatt tccctcctcc attcttttat tttgtttcta tttcttgcgt aaatctgatt
     2161 aaaatcgaga agtaggcgcg cattttctgt cttcaccttt tgactataaa agtactctac
     2221 aaatattgaa caacactttt taaaagttgt agtagttatt ggccatcagg aattaggagg
     2281 tcagttgcaa aatcatgcat cttttaggat taaaagctta aatcttgctc attcatttcc
     2341 tgtcttattt tgaagttttt ctctcttcca acgcctccca ctgtgactac tatcatctat
     2401 tattatcttg tagggattcc tcaatttcca gacaccctca aactcggccc cgttccgatt
     2461 cgaacccggg attggccaaa ggacccaaag gtatgtttcg aatgatacta acataacata
     2521 gaacattttc aggaggaccc ttgcttggag ggtaccgagc tcagaaaaaa tgaccgctcc
     2581 aaagaagaag cgcaaggttc cagtcgagaa gatgactgcc ccaaaaaaga aacgtaaggt
     2641 cccgcgggta agtttaaaca gttcggtact aactaaccat acatatttaa attttcagcg
     2701 cgctgtgtca aaaggagaag agcttattaa ggagaatatg cgttccaagc tctacctcga
     2761 gggatccgtc aacggacacc aattcaagtg cacccacgag ggagagggaa agccatacga
     2821 gggaaagcaa accaaccgta tcaaggtcgt cgagggagga ccactcccat tcgccttcga
     2881 catcctcgcc acccacttca tgtacggatc caaggtaagt ttaaacatat atatactaac
     2941 taaccctgat tatttaaatt ttcaggtctt catcaagtac ccagccgacc tcccagacta
     3001 cttcaagcaa tccttcccag agggattcac ctgggagcgt gtcatggtct tcgaggacgg
     3061 aggagtcctc accgccaccc aagacacctc cctccaagac ggagagctca tctacaacgt
     3121 caaggtccgt ggagtcaact tcccagccaa cggaccagtc atgcaaaaga agaccctcgg
     3181 atgggagcca tccaccgaga ccatgtaccc agccgacgga ggactcgagg gacgttgcga
     3241 caaggtaagt ttaaacatga ttttactaac taactaatct gatttaaatt ttcaggccct
     3301 caagctcgtc ggaggaggac acctccacgt caacttcaag accacctaca agtccaagaa
     3361 gccagtcaag atgccaggag tccactacgt cgaccgtcgt ctcgagcgta tcaaggaggc
     3421 cgacaacgag acctacgtcg agcaatacga gcacgccgtc gcccgttact ccaacctcgg
     3481 aggaggaatg gacgagctct acaagcctag gataccggtg ggtgaagacc caccaaagcc
     3541 atctgccaag ggagccaaga aggccgccaa gaccgttacg aagccaaagg acggaaagaa
     3601 gagacgtcat gcccgtaagg aatcatactc cgtctacatc taccgtgtcc tcaagcaagt
     3661 tcatccagac actggagttt cctccaaagc catgtctatc atgaactctt ttgtcaacga
     3721 tgtcttcgag cgtattgctg ctgaagcatc ccgtcttgct cactacaaca agcgttccac
     3781 aatctcatcc cgcgaaattc agaccgctgt ccgtctgatc cttccaggag agcttgccaa
     3841 gcacgccgtg tctgagggaa ccaaggccgt taccaagtac acttccagca agtaggaatt
     3901 ccaactgagc gccggtcgct accattacca acttgtctgg tgtcaaaaat aataggggcc
     3961 gctgtcatca gagtaagttt aaactgagtt ctactaacta acgagtaata tttaaatttt
     4021 cagcatctcg cgcccgtgcc tctgacttct aagtccaatt actcttcaac atccctacat
     4081 gctctttctc cctgtgctcc caccccctat ttttgttatt atcaaaaaac ttctcttaat
     4141 ttctttgttt tttagcttct tttaagtcac ctctaacaat gaaattgtgt agattcaaaa
     4201 atagaattaa ttcgtaataa aaagtcgaaa aaaattgtgc tccctccccc cattaataat
     4261 aattctatcc caaaatctac acaatgttct gtgtacactt cttatgtttt ttacttctga
     4321 taaatttttt tgaaacatca tagaaaaaac cgcacacaaa ataccttatc atatgttacg
     4381 tttcagttta tgaccgcaat ttttatttct tcgcacgtct gggcctctca tgacgtcaaa
     4441 tcatgctcat cgtgaaaaag ttttggagta tttttggaat ttttcaatca agtgaaagtt
     4501 tatgaaatta attttcctgc ttttgctttt tggggtttcc cctattgttt gtcaagattt
     4561 cgaggacggc gtttttcttg ctaaaatcac aagtattgat gagcacgatg caagaaagat
     4621 cggaagaagg tttgggtttg aggctcagtg gaaggtgagt agaagttgat aatttgaaag
     4681 tggagtagtg tctatggggt ttttgcctta aatgacagaa tacattccca atataccaaa
     4741 cataactgtt tcctactagt cggccgtacg ggccctttcg tctcgcgcgt ttcggtgatg
     4801 acggtgaaaa cctctgacac atgcagctcc cggagacggt cacagcttgt ctgtaagcgg
     4861 atgccgggag cagacaagcc cgtcagggcg cgtcagcggg tgttggcggg tgtcggggct
     4921 ggcttaacta tgcggcatca gagcagattg tactgagagt gcaccatatg cggtgtgaaa
     4981 taccgcacag atgcgtaagg agaaaatacc gcatcaggcg gccttaaggg cctcgtgata
     5041 cgcctatttt tataggttaa tgtcatgata ataatggttt cttagacgtc aggtggcact
     5101 tttcggggaa atgtgcgcgg aacccctatt tgtttatttt tctaaataca ttcaaatatg
     5161 tatccgctca tgagacaata accctgataa atgcttcaat aatattgaaa aaggaagagt
     5221 atgagtattc aacatttccg tgtcgccctt attccctttt ttgcggcatt ttgccttcct
     5281 gtttttgctc acccagaaac gctggtgaaa gtaaaagatg ctgaagatca gttgggtgca
     5341 cgagtgggtt acatcgaact ggatctcaac agcggtaaga tccttgagag ttttcgcccc
     5401 gaagaacgtt ttccaatgat gagcactttt aaagttctgc tatgtggcgc ggtattatcc
     5461 cgtattgacg ccgggcaaga gcaactcggt cgccgcatac actattctca gaatgacttg
     5521 gttgagtact caccagtcac agaaaagcat cttacggatg gcatgacagt aagagaatta
     5581 tgcagtgctg ccataaccat gagtgataac actgcggcca acttacttct gacaacgatc
     5641 ggaggaccga aggagctaac cgcttttttg cacaacatgg gggatcatgt aactcgcctt
     5701 gatcgttggg aaccggagct gaatgaagcc ataccaaacg acgagcgtga caccacgatg
     5761 cctgtagcaa tggcaacaac gttgcgcaaa ctattaactg gcgaactact tactctagct
     5821 tcccggcaac aattaataga ctggatggag gcggataaag ttgcaggacc acttctgcgc
     5881 tcggcccttc cggctggctg gtttattgct gataaatctg gagccggtga gcgtgggtct
     5941 cgcggtatca ttgcagcact ggggccagat ggtaagccct cccgtatcgt agttatctac
     6001 acgacgggga gtcaggcaac tatggatgaa cgaaatagac agatcgctga gataggtgcc
     6061 tcactgatta agcattggta actgtcagac caagtttact catatatact ttagattgat
     6121 ttaaaacttc atttttaatt taaaaggatc taggtgaaga tcctttttga taatctcatg
     6181 accaaaatcc cttaacgtga gttttcgttc cactgagcgt cagaccccgt agaaaagatc
     6241 aaaggatctt cttgagatcc tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa
     6301 ccaccgctac cagcggtggt ttgtttgccg gatcaagagc taccaactct ttttccgaag
     6361 gtaactggct tcagcagagc gcagatacca aatactgtcc ttctagtgta gccgtagtta
     6421 ggccaccact tcaagaactc tgtagcaccg cctacatacc tcgctctgct aatcctgtta
     6481 ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg ggttggactc aagacgatag
     6541 ttaccggata aggcgcagcg gtcgggctga acggggggtt cgtgcacaca gcccagcttg
     6601 gagcgaacga cctacaccga actgagatac ctacagcgtg agcattgaga aagcgccacg
     6661 cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg aacaggagag
     6721 cgcacgaggg agcttccagg gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc
     6781 cacctctgac ttgagcgtcg atttttgtga tgctcgtcag gggggcggag cctatggaaa
     6841 aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttt tgctcacatg
     6901 ttctttcctg cgttatcccc tgattctgtg gataaccgta ttaccgcctt tgagtgagct
     6961 gataccgctc gccgcagccg aacgaccgag cgcagcgagt cagtgagcga ggaagcggaa
     7021 gagcgcccaa tacgcaaacc gcctctcccc gcgcgttggc cgattcatta atgcagctgg
     7081 cacgacaggt ttcccgactg gaaagcgggc agtgagcgca acgcaattaa tgtgagttag
     7141 ctcactcatt aggcacccca ggctttacac tttatgcttc cggctcgtat gttgtgtgga
     7201 attgtgagcg gataacaatt
//
