LOCUS       45952_44                6425 bp DNA     circular SYN 04-APR-2017
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6425)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Sep 7, 2022 from SnapGene 6.1.1
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..6425
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     source          3161..4015
                     /mol_type="other DNA"
                     /label=source:pPD95_62
                     /organism="pPD95_62"
     primer_bind     6..22
                     /label=M13R_GW_PRIMER
     misc_feature    113..131
                     /label=EV Landmark FWD
     misc_feature    170..1669
                     /label=gpa-1
     misc_feature    170
                     /label=~Jansen 1999 FWD
     misc_feature    1491..1508
                     /label=EV gpa-1 End FWD
     misc_feature    1775..1816
                     /label=1xNLS
     misc_feature    1776..1795
                     /label=Color FWD
     misc_feature    1817..1852
                     /label=NLS2
     misc_feature    1847..1852
                     /label=SacII Cut Site
     misc_feature    1903..1908
                     /label=BssHII Cut Site
     misc_feature    join(1910..2119,2171..2449,2501..2710)
                     /label=CyOFP1
     misc_feature    1945..1962
                     /label=EV CyOFP Start REV
     misc_feature    2218..2236
                     /label=EV CYOFP Mid2 FWD
     misc_feature    2285..2303
                     /label=EV CyOFP1 Mid FWD
     misc_feature    2337..2354
                     /label=EV CyOFP1 Mid REV
     misc_feature    2395..2413
                     /label=Ev CYOFP Mid3 FWD
     misc_feature    2525..2543
                     /label=EV CYOFP Mid3 REV
     misc_feature    2530..2548
                     /label=EV CyOFP Mid2 REV
     misc_feature    2711..2716
                     /label=AvrII Cut Site
     misc_feature    complement(2717..2718)
                     /label=Frame Link
     misc_feature    complement(2719..2724)
                     /label=AgeI Cut Site
     misc_feature    2735..3100
                     /label=H2B
     misc_feature    2882..2901
                     /label=H2B Mid REV
     misc_feature    3101..3106
                     /label=EcoRI Cut Site
     misc_feature    3107..3160
                     /label=Ligated Addition
     misc_feature    3229..3956
                     /label=unc-54 3'UTR
     misc_feature    3239..3257
                     /label=EV unc-54 3'UTR REV
     misc_feature    3707..3859
                     /label=aex-5 Exon1
     primer_bind     complement(3957..3982)
                     /label=Primer D54
     primer_bind     complement(3977..4002)
                     /label=PrimerD858
     misc_feature    3994..4011
                     /label=EV Landmark REV
     primer_bind     complement(4016..4038)
                     /label=3GEX_GW_PRIMER
     misc_feature    4016..4033
                     /label=EV ceBFP2 + H2B GIB REV
     CDS             4624..4990
                     /label=AmpR
     CDS             4991..5283
                     /label=AmpR
                     /label=AmpR(1)
     rep_origin      5381..6063
                     /label=ColE1 origin
     misc_binding    6403..6425
                     /label=LacO
ORIGIN
        1 tcacacagga aacagctatg accatgatta cgccaagctg taagtttaaa catgatctta
       61 ctaactaact attctcattt aaattttcag agcttaaaaa tggctgaaat cactcacaac
      121 gatggatacg ctaacaactt ggaaatgaaa taagcttgca tgcctgcagt cgtttcctgg
      181 aacttgtgaa gaatttttct gcaacaacgt ggtaaataat aaaagctttc caaataagtt
      241 atcaggtcgt cgccaggaaa ttgctaggaa attgctaggt ttcaaggttg tcagaaagta
      301 gaaaaagttt gacaacgtat ttaaatttca aattatgcct aaagaataaa tatctagact
      361 ttatgaattt ttttttctat gtacctttaa accctcacaa atatatcccc ttgttctctg
      421 gttacgttga cttttttact atataatttt cgaaaaacct tgtcttgagt atcatcttac
      481 tttgtttgtc aatagtaatt tttctttcat ttttctttac aatatttcgt ttcccgaaca
      541 tttgaaatat aagccaaatt ttggttttca ttgagaaaac tctacatttc ttgaaaaaaa
      601 actacctgaa tttccagtaa tttctcatct tcatataatt ctaaaaacta aatttttaat
      661 gtcatgagag aattgaaaag tagttgggga aattaaattt cacctgcagt tgagagagta
      721 tagaaggact caacgcttac attctttatg acgtatcagc ttgaataagt atttttccag
      781 acttatatca gactaagttg aaaaacgggc agtactttta tttaatttat tcttgtgtac
      841 tttgtttctt tgagatcaag ttataagcac tattttataa ccaactttta aatattgtta
      901 tatttttctg ggaagtgaat tgattaaata agttttaaat ctttgagaag ttagcaatac
      961 aaatataatg tgagataatt gtgaacaaga atttaatatt gcttttgaat ctgtattaaa
     1021 caaacgatga aatattagaa gttaaatttg aacattgtgg tacaatacta ataaatgaaa
     1081 tacggttggt attaagtaaa atatgagtca gtatatccct ttaaaatcca tttttatttt
     1141 aaaatctgta tatttctgtg aagcctgccg ctgtttatat gttaaaaagg aaggttttac
     1201 tttctcaaat acttaatatc aggtttcata caatttttga aaaaaaaacg acaaatcttc
     1261 agttgtgctt ttttctttct ccatcttgtc atcatttcat cattttcaat gtattctata
     1321 attatttcca gctgccatta aatgcaaccc actttaatat ttttcttcct tttttccgtc
     1381 tgttccgaaa cccttgaaat cgactttttc attcactgaa ttcactttct ctgcctcgag
     1441 ttgtttatgc ggcgaaaaaa aatctgtaga ggtatacgaa aaagctaatt aggtggtttg
     1501 ccgtctagta atactcgatt atatatttct tgtcgtcatc gttaccactg ctctccattt
     1561 tctctcctca ctcacaaaaa cttgaatatt cttcgcttcc tgttgtgatc agtgaaaatt
     1621 cgtctgattt cttcaatatt attcaatgtc atttattcga agacttcagc ccgggattgg
     1681 ccaaaggacc caaaggtatg tttcgaatga tactaacata acatagaaca ttttcaggag
     1741 gacccttgct tggagggtac cgagctcaga aaaaatgacc gctccaaaga agaagcgcaa
     1801 ggttccagtc gagaagatga ctgccccaaa aaagaaacgt aaggtcccgc gggtaagttt
     1861 aaacagttcg gtactaacta accatacata tttaaatttt cagcgcgctg tgtcaaaagg
     1921 agaagagctt attaaggaga atatgcgttc caagctctac ctcgagggat ccgtcaacgg
     1981 acaccaattc aagtgcaccc acgagggaga gggaaagcca tacgagggaa agcaaaccaa
     2041 ccgtatcaag gtcgtcgagg gaggaccact cccattcgcc ttcgacatcc tcgccaccca
     2101 cttcatgtac ggatccaagg taagtttaaa catatatata ctaactaacc ctgattattt
     2161 aaattttcag gtcttcatca agtacccagc cgacctccca gactacttca agcaatcctt
     2221 cccagaggga ttcacctggg agcgtgtcat ggtcttcgag gacggaggag tcctcaccgc
     2281 cacccaagac acctccctcc aagacggaga gctcatctac aacgtcaagg tccgtggagt
     2341 caacttccca gccaacggac cagtcatgca aaagaagacc ctcggatggg agccatccac
     2401 cgagaccatg tacccagccg acggaggact cgagggacgt tgcgacaagg taagtttaaa
     2461 catgatttta ctaactaact aatctgattt aaattttcag gccctcaagc tcgtcggagg
     2521 aggacacctc cacgtcaact tcaagaccac ctacaagtcc aagaagccag tcaagatgcc
     2581 aggagtccac tacgtcgacc gtcgtctcga gcgtatcaag gaggccgaca acgagaccta
     2641 cgtcgagcaa tacgagcacg ccgtcgcccg ttactccaac ctcggaggag gaatggacga
     2701 gctctacaag cctaggatac cggtgggtga agacccacca aagccatctg ccaagggagc
     2761 caagaaggcc gccaagaccg ttacgaagcc aaaggacgga aagaagagac gtcatgcccg
     2821 taaggaatca tactccgtct acatctaccg tgtcctcaag caagttcatc cagacactgg
     2881 agtttcctcc aaagccatgt ctatcatgaa ctcttttgtc aacgatgtct tcgagcgtat
     2941 tgctgctgaa gcatcccgtc ttgctcacta caacaagcgt tccacaatct catcccgcga
     3001 aattcagacc gctgtccgtc tgatccttcc aggagagctt gccaagcacg ccgtgtctga
     3061 gggaaccaag gccgttacca agtacacttc cagcaagtag gaattccaac tgagcgccgg
     3121 tcgctaccat taccaacttg tctggtgtca aaaataatag gggccgctgt catcagagta
     3181 agtttaaact gagttctact aactaacgag taatatttaa attttcagca tctcgcgccc
     3241 gtgcctctga cttctaagtc caattactct tcaacatccc tacatgctct ttctccctgt
     3301 gctcccaccc cctatttttg ttattatcaa aaaacttctc ttaatttctt tgttttttag
     3361 cttcttttaa gtcacctcta acaatgaaat tgtgtagatt caaaaataga attaattcgt
     3421 aataaaaagt cgaaaaaaat tgtgctccct ccccccatta ataataattc tatcccaaaa
     3481 tctacacaat gttctgtgta cacttcttat gttttttact tctgataaat ttttttgaaa
     3541 catcatagaa aaaaccgcac acaaaatacc ttatcatatg ttacgtttca gtttatgacc
     3601 gcaattttta tttcttcgca cgtctgggcc tctcatgacg tcaaatcatg ctcatcgtga
     3661 aaaagttttg gagtattttt ggaatttttc aatcaagtga aagtttatga aattaatttt
     3721 cctgcttttg ctttttgggg tttcccctat tgtttgtcaa gatttcgagg acggcgtttt
     3781 tcttgctaaa atcacaagta ttgatgagca cgatgcaaga aagatcggaa gaaggtttgg
     3841 gtttgaggct cagtggaagg tgagtagaag ttgataattt gaaagtggag tagtgtctat
     3901 ggggtttttg ccttaaatga cagaatacat tcccaatata ccaaacataa ctgtttccta
     3961 ctagtcggcc gtacgggccc tttcgtctcg cgcgtttcgg tgatgacggt gaaaacctct
     4021 gacacatgca gctcccggag acggtcacag cttgtctgta agcggatgcc gggagcagac
     4081 aagcccgtca gggcgcgtca gcgggtgttg gcgggtgtcg gggctggctt aactatgcgg
     4141 catcagagca gattgtactg agagtgcacc atatgcggtg tgaaataccg cacagatgcg
     4201 taaggagaaa ataccgcatc aggcggcctt aagggcctcg tgatacgcct atttttatag
     4261 gttaatgtca tgataataat ggtttcttag acgtcaggtg gcacttttcg gggaaatgtg
     4321 cgcggaaccc ctatttgttt atttttctaa atacattcaa atatgtatcc gctcatgaga
     4381 caataaccct gataaatgct tcaataatat tgaaaaagga agagtatgag tattcaacat
     4441 ttccgtgtcg cccttattcc cttttttgcg gcattttgcc ttcctgtttt tgctcaccca
     4501 gaaacgctgg tgaaagtaaa agatgctgaa gatcagttgg gtgcacgagt gggttacatc
     4561 gaactggatc tcaacagcgg taagatcctt gagagttttc gccccgaaga acgttttcca
     4621 atgatgagca cttttaaagt tctgctatgt ggcgcggtat tatcccgtat tgacgccggg
     4681 caagagcaac tcggtcgccg catacactat tctcagaatg acttggttga gtactcacca
     4741 gtcacagaaa agcatcttac ggatggcatg acagtaagag aattatgcag tgctgccata
     4801 accatgagtg ataacactgc ggccaactta cttctgacaa cgatcggagg accgaaggag
     4861 ctaaccgctt ttttgcacaa catgggggat catgtaactc gccttgatcg ttgggaaccg
     4921 gagctgaatg aagccatacc aaacgacgag cgtgacacca cgatgcctgt agcaatggca
     4981 acaacgttgc gcaaactatt aactggcgaa ctacttactc tagcttcccg gcaacaatta
     5041 atagactgga tggaggcgga taaagttgca ggaccacttc tgcgctcggc ccttccggct
     5101 ggctggttta ttgctgataa atctggagcc ggtgagcgtg ggtctcgcgg tatcattgca
     5161 gcactggggc cagatggtaa gccctcccgt atcgtagtta tctacacgac ggggagtcag
     5221 gcaactatgg atgaacgaaa tagacagatc gctgagatag gtgcctcact gattaagcat
     5281 tggtaactgt cagaccaagt ttactcatat atactttaga ttgatttaaa acttcatttt
     5341 taatttaaaa ggatctaggt gaagatcctt tttgataatc tcatgaccaa aatcccttaa
     5401 cgtgagtttt cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga
     5461 gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg
     5521 gtggtttgtt tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc
     5581 agagcgcaga taccaaatac tgtccttcta gtgtagccgt agttaggcca ccacttcaag
     5641 aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc
     5701 agtggcgata agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg
     5761 cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac
     5821 accgaactga gatacctaca gcgtgagcat tgagaaagcg ccacgcttcc cgaagggaga
     5881 aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt
     5941 ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag
     6001 cgtcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg
     6061 gcctttttac ggttcctggc cttttgctgg ccttttgctc acatgttctt tcctgcgtta
     6121 tcccctgatt ctgtggataa ccgtattacc gcctttgagt gagctgatac cgctcgccgc
     6181 agccgaacga ccgagcgcag cgagtcagtg agcgaggaag cggaagagcg cccaatacgc
     6241 aaaccgcctc tccccgcgcg ttggccgatt cattaatgca gctggcacga caggtttccc
     6301 gactggaaag cgggcagtga gcgcaacgca attaatgtga gttagctcac tcattaggca
     6361 ccccaggctt tacactttat gcttccggct cgtatgttgt gtggaattgt gagcggataa
     6421 caatt
//
