LOCUS       45952_74                5388 bp DNA     circular SYN 09-FEB-2017
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5388)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Sep 7, 2022 from SnapGene 6.1.1
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..5388
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     6..22
                     /label=M13R_GW_PRIMER
     misc_feature    113..131
                     /label=EV Landmark FWD
     misc_feature    169..924
                     /label=glr-1
     misc_feature    169..889
                     /label=glr-1 Prom Hart 1995
     misc_feature    169..243
                     /label=flp-3
     misc_feature    169..204
                     /label=flp-3 exons
     misc_feature    712..730
                     /label=EV glr-1 End FWD
     misc_feature    951..992
                     /label=artificial intron
     misc_feature    1007..1012
                     /label=KpnI Cut Site
     primer_bind     1013..1030
                     /label=M13F-41_GW_PRIMER
     misc_feature    1029..1034
                     /label=MluI Cut Site
                     /label=MluI Cut Site(1)
     misc_feature    1035..1040
                     /label=KpnI Cut Site
                     /label=KpnI Cut Site(1)
     misc_feature    1054..1134
                     /label=2xNLS
     misc_feature    1173..1178
                     /label=MluI Cut Site
     misc_feature    1175..1195
                     /label=EV NLS3 intron REV
     misc_feature    1180..1182
                     /label=ORF ATG
     misc_feature    1186..1236
                     /label=intron
     misc_feature    1236..1241
                     /label=BssHII Cut Site
     CDS             join(1309..1332,1335..1371,1373,1375..1470,1498..1503,
                     1555..1729,1732..1869,1927..2076)
                     /codon_start=1
                     /label=mCardinal
                     /translation="MNHHFKCTRRGGQALRGHPDQGLRWWREAPCRSHSTSWPPALCTG
                     ARPSSTTPPSLRASHGRESPHTKTGACLPLPRTPASRTAA*STTSSSEG*TSHPTAL*C
                     RRKHSAGRPTETLYPADGGLEGRCDMALKLVGGGHLHCNLKTTYRSKKPAKNLKMVYFV
                     DRRLERIKEADNETYVEQHEVAVARYCDLPSKLGHKLNGMDELYK"
     misc_feature    1435..1437
                     /label=Same as mBeRFP Start
     misc_feature    1438..1455
                     /label=EV mNeptune2.5 Mid FWD
     misc_feature    1504..1554
                     /label=intron 2
     misc_feature    1549..1566
                     /label=EV mBeRFP/mNep2.5 intron3 Mid FWD
     misc_feature    1555..1561
                     /label=Bsu36I Cut Site
     misc_feature    1561..1578
                     /label=EV mNeptune2.5 Mid REV
     misc_feature    1576..1578
                     /label=Same as mBeRFP End
     misc_feature    1842..1861
                     /label=EV mCardinal Mid REV
     misc_feature    1863..1885
                     /label=EV mNeptune2.5 intron MID REV
     misc_feature    1876..1926
                     /label=intron 3
     misc_feature    1927..1932
                     /label=AccI Cut Site
     misc_feature    2050..2052
                     /label=Last mChOpti Difference
     misc_feature    2077..2899
                     /label=unc-54 3' UTR
     misc_feature    2091..2112
                     /label=EV unc-54 3'UTR GIB REV
     misc_feature    2202..2220
                     /label=EV unc-54 3'UTR REV
     misc_feature    2957..2974
                     /label=EV Landmark REV
     primer_bind     complement(2979..3001)
                     /label=3GEX_GW_PRIMER
     CDS             3587..4246
                     /label=AmpR
     rep_origin      4344..5026
                     /label=ColE1 origin
     misc_binding    5366..5388
                     /label=LacO
ORIGIN
        1 tcacacagga aacagctatg accatgatta cgccaagctg taagtttaaa catgatctta
       61 ctaactaact attctcattt aaattttcag agcttaaaaa tggctgaaat cactcacaac
      121 gatggatacg ctaacaactt ggaaatgaaa taagcttgca tgcctgcagg agtcatcatt
      181 ggagctgaac gtccaaagaa ataaatgaat ttgtttattg aataaatact attgagcatc
      241 gaatatttgt tctgaaaaga tcacatcgta aatttaaaac actgttttct cactttttaa
      301 tggaaaatat tcaattcttt gaaacttcgt ggaagctgaa aagtatcaga acacaaactc
      361 taaattttcc tgggggcttc tcccgcaact atgaaaatat aaatttccat aacttgttca
      421 ttccaaacct tatttcatca aattcagtca aaagaggttc cttttcatcc gctcgtccgt
      481 gtcccgaaaa tgaagatgtg ctgccctccc atccaagtgt ctctccctgt gtatgggtgg
      541 gggacgttga ttggccattt ctgtctacgt ctcttcttct catacactat tcaaatgctc
      601 agaggtgagt ctgaaatgca atttagggtt tgcgagtgct cggctccctc cgctcctact
      661 ctttacacac ttggctgcgg gcggacgcga aggtcctcca atttttcttc gtctcggtca
      721 cttcacttcg tactttttca atttcgccga aataagacta ttcttcctta gctgacttct
      781 ttcttgtact ctcacccttt ctaagacatg tgttaatccg aaagaacatc caattttctg
      841 tgtacaatga agtgaaggat caaatttccg agaagaaagg caaagcaagt cgacgtcgcc
      901 ggcacccaat ctgacacatt cacacccggg attggccaaa ggacccaaag gtatgtttcg
      961 aatgatacta acataacata gaacattttc aggaggaccc ttggagggta ccggttttcc
     1021 cagtcacgac gcgtggtacc gagctcagaa aaaatgaccg ctccaaagaa gaaacgcaaa
     1081 gtaccgagct cagaaaaaat gacggcacct aaaaagaaac gcaaagtacc ggtcgccgag
     1141 aagacagccc caaaaaagaa gcgtaaagtt ccacgcgtca tgggagtaag tttaaacagt
     1201 tcggtactaa ctaaccatac atatttaaat tttcagcgcg ctgtctccaa gggagaggag
     1261 ctcatcaagg agaacatgca caccaagctc tacatggagg gaaccgtcaa caaccaccac
     1321 ttcaagtgca cccacgaagg ggagggcaag ccctacgagg gcacccagac caacaggatt
     1381 aaggtggtgg agggaggccc cctgccgttc gcattcgaca tcctggccac ctgctttatg
     1441 tacgggagca agaccttcat caaccacacc caaggaatcc cagacttctt caagcaatcc
     1501 ttcgtaagtt taaacatgat tttactaact aactaatctg atttaaattt tcagcctgag
     1561 ggcttcacat gggagagagt caccacatac gaagacgggg gcgtgcttac cgttacccag
     1621 gacaccagcc tccaggacgg ctgcttgatc tacaacgtca agctcagagg ggtgaacttc
     1681 ccatccaacg gccctgtgat gcagaagaaa acactcggct gggaggccag taccgagacc
     1741 ctgtaccccg ctgacggcgg cctggaaggc agatgcgaca tggccctgaa gctcgtgggc
     1801 gggggccacc tgcactgcaa cctgaagacc acatacagat ccaagaaacc cgctaagaac
     1861 ctcaagatgc caggagtaag tttaaacata tatatactaa ctaaccctga ttatttaaat
     1921 tttcaggtct actttgtgga ccgcagactg gaaagaatca aggaggccga caatgagacc
     1981 tacgtcgagc agcacgaggt ggctgtggcc agatactgcg acctccctag caaactgggg
     2041 cacaaactta atggcatgga cgagctgtac aagtaagcgc cggtcgctac cattaccaac
     2101 ttgtctggtg tcaaaaataa taggggccgc tgtcatcaga gtaagtttaa actgagttct
     2161 actaactaac gagtaatatt taaattttca gcatctcgcg cccgtgcctc tgacttctaa
     2221 gtccaattac tcttcaacat ccctacatgc tctttctccc tgtgctccca ccccctattt
     2281 ttgttattat caaaaaactt ctcttaattt ctttgttttt tagcttcttt taagtcacct
     2341 ctaacaatga aattgtgtag attcaaaaat agaattaatt cgtaataaaa agtcgaaaaa
     2401 aattgtgctc cctcccccca ttaataataa ttctatccca aaatctacac aatgttctgt
     2461 gtacacttct tatgtttttt acttctgata aatttttttg aaacatcata gaaaaaaccg
     2521 cacacaaaat accttatcat atgttacgtt tcagtttatg accgcaattt ttatttcttc
     2581 gcacgtctgg gcctctcatg acgtcaaatc atgctcatcg tgaaaaagtt ttggagtatt
     2641 tttggaattt ttcaatcaag tgaaagttta tgaaattaat tttcctgctt ttgctttttg
     2701 gggtttcccc tattgtttgt caagatttcg aggacggcgt ttttcttgct aaaatcacaa
     2761 gtattgatga gcacgatgca agaaagatcg gaagaaggtt tgggtttgag gctcagtgga
     2821 aggtgagtag aagttgataa tttgaaagtg gagtagtgtc tatggggttt ttgccttaaa
     2881 tgacagaata cattcccaat ataccaaaca taactgtttc ctactagtcg gccgtacggg
     2941 ccctttcgtc tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg
     3001 gagacggtca cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg
     3061 tcagcgggtg ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta
     3121 ctgagagtgc accatatgcg gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc
     3181 atcaggcggc cttaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat
     3241 aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg
     3301 tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat
     3361 gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat
     3421 tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt
     3481 aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag
     3541 cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa
     3601 agttctgcta tgtggcgcgg tattatcccg tattgacgcc gggcaagagc aactcggtcg
     3661 ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct
     3721 tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac
     3781 tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca
     3841 caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat
     3901 accaaacgac gagcgtgaca ccacgatgcc tgtagcaatg gcaacaacgt tgcgcaaact
     3961 attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc
     4021 ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga
     4081 taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg
     4141 taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg
     4201 aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca
     4261 agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta
     4321 ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca
     4381 ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg
     4441 cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga
     4501 tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa
     4561 tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc
     4621 tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg
     4681 tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac
     4741 ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct
     4801 acagcgtgag cattgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc
     4861 ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg
     4921 gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg
     4981 ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct
     5041 ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga
     5101 taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg
     5161 cagcgagtca gtgagcgagg aagcggaaga gcgcccaata cgcaaaccgc ctctccccgc
     5221 gcgttggccg attcattaat gcagctggca cgacaggttt cccgactgga aagcgggcag
     5281 tgagcgcaac gcaattaatg tgagttagct cactcattag gcaccccagg ctttacactt
     5341 tatgcttccg gctcgtatgt tgtgtggaat tgtgagcgga taacaatt
//
