LOCUS       45952_84                7788 bp DNA     circular SYN 22-MAY-2017
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7788)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Sep 7, 2022 from SnapGene 6.1.1
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..7788
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     source          join(4088..4097,4524..5378)
                     /mol_type="other DNA"
                     /label=source:pPD95_62
                     /organism="pPD95_62"
     primer_bind     6..22
                     /label=M13R_GW_PRIMER
     misc_feature    113..131
                     /label=EV Landmark FWD
     misc_feature    170..1940
                     /label=odr-1
     misc_feature    170..216
                     /label=glb-20
     misc_feature    170..204
                     /label=glb-20 exons
     misc_feature    1861..1878
                     /label=EV odr-1 End FWD
     misc_feature    1952..1971
                     /label=EV pPD95 MCS FWD
     misc_feature    2023..2028
                     /label=KpnI Cut Site
     primer_bind     2029..2046
                     /label=M13F-41_GW_PRIMER
     misc_feature    2045..2050
                     /label=MluI Cut Site
     misc_feature    2051..2056
                     /label=KpnI Cut Site
                     /label=KpnI Cut Site(1)
     misc_feature    2070..2197
                     /label=3xNLS
     misc_feature    2189..2194
                     /label=MluI Cut Site
                     /label=MluI Cut Site(1)
     misc_feature    2191..2211
                     /label=EV NLS3 intron REV
     misc_feature    2196..2198
                     /label=ORF ATG
     misc_feature    2252..2257
                     /label=BssHII Cut Site
     misc_feature    join(2259..2519,2571..2891,2943..3089)
                     /label=mNeptune2.5
     misc_feature    2451..2453
                     /label=Same as mBeRFP Start
     misc_feature    2454..2471
                     /label=EV mNeptune2.5 Mid FWD
     misc_feature    2565..2582
                     /label=EV mBeRFP/mNep2.5 intron3 Mid FWD
     misc_feature    2571..2577
                     /label=Bsu36I Cut Site
     misc_feature    2577..2594
                     /label=EV mNeptune2.5 Mid REV
     misc_feature    2592..2594
                     /label=Same as mBeRFP End
     misc_feature    2858..2877
                     /label=EV mCardinal Mid REV
     misc_feature    2879..2901
                     /label=EV mNeptune2.5 intron MID REV
     misc_feature    2943..2948
                     /label=AccI Cut Site
     misc_feature    3066..3068
                     /label=Last mChOpti Difference
     misc_feature    3090..3143
                     /label=T2A
     misc_feature    3128..3143
                     /label=EV T2mBeRFP T2A REV
     misc_feature    3128..3143
                     /label=EV T2mBeRFP T2A FWD
     misc_feature    3144..3185
                     /label=1xNLS
     misc_feature    3145..3164
                     /label=Color FWD
     misc_feature    3186..3221
                     /label=NLS2
     misc_feature    join(3222..3395,3447..3629,3681..3872,3924..4073)
                     /label=ceBFP2
     misc_feature    3275..3296
                     /label=EV ceBFP2 Start REV
     misc_feature    3555..3572
                     /label=EV ceBFP2 Mid FWD
     misc_feature    3603..3622
                     /label=EV ceBFP2 Mid REV
     misc_feature    3710..3728
                     /label=EV ceBFP2 Mid2 FWD
     misc_feature    3836..3853
                     /label=EV ceBFP2 Mid2 REV
     misc_feature    3843..3845
                     /label=I to A mTagBFP2 Fix
     misc_feature    complement(4074..4079)
                     /label=Linker AvrII Cut Site
     misc_feature    complement(4080..4081)
                     /label=Frame Link
     misc_feature    complement(4082..4087)
                     /label=AgeI Cut Site
     misc_feature    4098..4463
                     /label=H2B
     misc_feature    4245..4264
                     /label=H2B Mid REV
     misc_feature    4464..4469
                     /label=EcoRI Cut Site
     misc_feature    4470..4523
                     /label=Ligated Addition
     misc_feature    4592..5319
                     /label=unc-54 3'UTR
     misc_feature    4602..4620
                     /label=EV unc-54 3'UTR REV
     misc_feature    5070..5222
                     /label=aex-5 Exon1
     primer_bind     complement(5320..5345)
                     /label=Primer D54
     primer_bind     complement(5340..5365)
                     /label=PrimerD858
     misc_feature    5357..5374
                     /label=EV Landmark REV
     primer_bind     complement(5379..5401)
                     /label=3GEX_GW_PRIMER
     misc_feature    5379..5396
                     /label=EV ceBFP2 + H2B GIB REV
     CDS             5987..6353
                     /label=AmpR
     CDS             6354..6646
                     /label=AmpR
                     /label=AmpR(1)
     rep_origin      6744..7426
                     /label=ColE1 origin
     misc_binding    7766..7788
                     /label=LacO
ORIGIN
        1 tcacacagga aacagctatg accatgatta cgccaagctg taagtttaaa catgatctta
       61 ctaactaact attctcattt aaattttcag agcttaaaaa tggctgaaat cactcacaac
      121 gatggatacg ctaacaactt ggaaatgaaa taagcttgca tgcctgcagc aagcagcaca
      181 accagacgcc cgatcttcga ataatttata aaacatataa ttatgggtac aacaatttct
      241 cattcaaatt tttacaataa ccaactttct ttcttccttt ccgtttccct tgtttttttt
      301 cttattttct cctggaaaac atactgttgt tcaattcata ttttgctatt tctcgcttca
      361 aacttgtgaa accgtattcc acacttgcca tcaggctata ttttgcaatg aacattttat
      421 ttggtggctc gagtgctatt tgtaaaatag aaaacagaac ggaaattgaa gcaattcttc
      481 catcaatctt tttttttata tattggagat gaatcgaaaa tttttgaatg atatatggaa
      541 agtctgatac atagggaaaa aaagaaatta aattgaaccc aactagagag atcaaacgat
      601 ccatctataa aaaacggaaa ccgtgagaaa tacgctattg gaataaaaag ggcttgtgtg
      661 ctctttatga atgcgtgaat aaggtaactc aatgactttc aaaacaaata aataaaacct
      721 tgaaaaattg cgtcaaaaat gtgcagaaaa acgatgaaag gggtataaga aatatagggg
      781 aaaatgtaac taataatgat tattgtatta cctcaaatag tattgaatgc aaaaataatg
      841 gttttcatgt ttactttgcg ctaaaaatga cgaatttgct aaagtctcga ctaaatattt
      901 ctcgttaggc gagaagtcag tgctgaccta atagaggctg caagactaat tttcggttga
      961 cttgcaaggg gggcaaaact aatattgaaa gacggtagtt agactttcaa ttgaatgaat
     1021 ttcacaccgt gtgcagaaat gataaaacag tatcaacata tataagtata atttgtttac
     1081 tttttgctaa acaatattac atccagtaat atgaaaaaat gatcaacgcg taaaacttcc
     1141 gtactttaca agaaacaggg caagtgaaaa aaaaacattt ctgttaccac atgtttccaa
     1201 caattgaagc aattcttgta atagataaca atacaaaatg taatcagaaa ttcagtggga
     1261 gtatccgctc cggaagtagt tgattggcca gcattatacc caaaatttat gagctaaatg
     1321 tattgtgtat tcgtaacatc atatttcttg cgagttttaa tttgattatc agaataacgg
     1381 taagtaaaat atgcttcatt gagctgaaaa ttaaattcaa aaaacacatg cgctcaaaca
     1441 actaattcta tctgaataca ttaagcagtt tcctgatcaa ttgttttgca tttatcaaca
     1501 tactttattt caaaaaaagt tagattcaaa ttttttttca gataacaatt tcgagaacct
     1561 gccttcatac agtaactatt caataaaatt ttatagtttg tcatattaat ttgtaacaag
     1621 tacagttatc taatttactt tgaactactt gattttatag ttaaggaagg taaaaaatcc
     1681 tactatacta atccatgtat tcattgcaaa gcccactaga ttcgtaatcc gttttgagtc
     1741 agctattcaa ctggaacaat atcatatcta ttctatgatt aaatacctat tcatttcaaa
     1801 atcttctccc ccacctattc actcttgcca tatttgatat atactttatc gatcttttca
     1861 tatcccttgt gagcaccctt gttttcatgc ttgaactcac aagttagctc attactgatt
     1921 attatcacct gaaaccctca cccgggattg gccaaaggac ccaaaggtat gtttcgaatg
     1981 atactaacat aacatagaac attttcagga ggacccttgg agggtaccgg ttttcccagt
     2041 cacgacgcgt ggtaccgagc tcagaaaaaa tgaccgctcc aaagaagaaa cgcaaagtac
     2101 cgagctcaga aaaaatgacg gcacctaaaa agaaacgcaa agtaccggtc gccgagaaga
     2161 cagccccaaa aaagaagcgt aaagttccac gcgtcatggg agtaagttta aacagttcgg
     2221 tactaactaa ccatacatat ttaaattttc agcgcgctgt ctccaaggga gaggagctca
     2281 tcaaggagaa catgcacacc aagctctaca tggagggaac cgtcaacaac caccacttca
     2341 agtgcaccca cgaaggggag ggcaagccct acgagggcac ccagaccaac aggattaagg
     2401 tggtggaggg aggccccctg ccgttcgcat tcgacatcct ggccacctgc tttatgtacg
     2461 ggagcaagac cttcatcaac cacacccaag gaatcccaga cttcttcaag caatccttcg
     2521 taagtttaaa catgatttta ctaactaact aatctgattt aaattttcag cctgagggct
     2581 tcacatggga gagagtcacc acatacgaag acgggggcgt gcttaccgtt acccaggaca
     2641 ccagcctcca ggacggctgc ttgatctaca acgtcaagct cagaggggtg aacttcccat
     2701 ccaacggccc tgtgatgcag aagaaaacac tcggctggga ggccagtacc gagaccctgt
     2761 accccgctga cggcggcctg gaaggcagat gcgacatggc cctgaagctc gtgggcgggg
     2821 gccacctgca ctgcaacctg aagaccacat acagatccaa gaaacccgct aagaacctca
     2881 agatgccagg agtaagttta aacatatata tactaactaa ccctgattat ttaaattttc
     2941 aggtctactt tgtggaccgc agactggaaa gaatcaagga ggccgacaat gagacctacg
     3001 tcgagcagca cgaggtggct gtggccagat actgcgacct ccctagcaaa ctggggcaca
     3061 aacttaatgg catggacgag ctgtacaagg agggacgtgg atccctcctc acctgcggag
     3121 acgtcgagga gaacccagga ccaatgaccg ctccaaagaa gaagcgcaag gttccagtcg
     3181 agaagatgac tgccccaaaa aagaaacgta aggtcccgcg gatgtcagag cttattaagg
     3241 agaatatgca tatgaaactg tacatggaag gaaccgtgga caaccatcac tttaagtgca
     3301 cttcggaggg ggaaggcaaa ccgtacgagg gcacccagac catgcgaatc aaggtggtcg
     3361 agggaggccc actgcccttc gccttcgaca ttctggtaag tttaaacata tatatactaa
     3421 ctaaccctga ttatttaaat tttcaggcta ctagcttcct gtacggcagc aagactttta
     3481 ttaatcacac ccagggcatc ccggacttct tcaaacagtc gttccctgag ggcttcacat
     3541 gggagcgtgt gaccacatac gaagacggtg gcgttctcac cgctacccag gacaccagcc
     3601 tccaggacgg ttgcctcata tataatgtgg taagtttaaa cagttcggta ctaactaacc
     3661 atacatattt aaattttcag aagatccggg gagtaaactt cacatccaat ggtccggtta
     3721 tgcagaagaa aacactcggc tgggaggcct tcaccgagac gctgtatccg gctgacggag
     3781 gcctggaagg tcgaaacgac atggccctga agctcgtcgg cggtagccat ctcatcgcaa
     3841 acgctaagac cacatataga tccaagaaac cggtaagttt aaacatgatt ttactaacta
     3901 actaatctga tttaaatttt caggctaaga accttaaaat gccgggagtc tactatgttg
     3961 actacagact ggaacggatc aaggaggcca acaacgagac ctacgtcgag cagcacgagg
     4021 ttgcagttgc cagatactgc gacctcccga gcaaactggg tcacaagctt aatcctagga
     4081 taccggtggg tgaagaccca ccaaagccat ctgccaaggg agccaagaag gccgccaaga
     4141 ccgttacgaa gccaaaggac ggaaagaaga gacgtcatgc ccgtaaggaa tcatactccg
     4201 tctacatcta ccgtgtcctc aagcaagttc atccagacac tggagtttcc tccaaagcca
     4261 tgtctatcat gaactctttt gtcaacgatg tcttcgagcg tattgctgct gaagcatccc
     4321 gtcttgctca ctacaacaag cgttccacaa tctcatcccg cgaaattcag accgctgtcc
     4381 gtctgatcct tccaggagag cttgccaagc acgccgtgtc tgagggaacc aaggccgtta
     4441 ccaagtacac ttccagcaag taggaattcc aactgagcgc cggtcgctac cattaccaac
     4501 ttgtctggtg tcaaaaataa taggggccgc tgtcatcaga gtaagtttaa actgagttct
     4561 actaactaac gagtaatatt taaattttca gcatctcgcg cccgtgcctc tgacttctaa
     4621 gtccaattac tcttcaacat ccctacatgc tctttctccc tgtgctccca ccccctattt
     4681 ttgttattat caaaaaactt ctcttaattt ctttgttttt tagcttcttt taagtcacct
     4741 ctaacaatga aattgtgtag attcaaaaat agaattaatt cgtaataaaa agtcgaaaaa
     4801 aattgtgctc cctcccccca ttaataataa ttctatccca aaatctacac aatgttctgt
     4861 gtacacttct tatgtttttt acttctgata aatttttttg aaacatcata gaaaaaaccg
     4921 cacacaaaat accttatcat atgttacgtt tcagtttatg accgcaattt ttatttcttc
     4981 gcacgtctgg gcctctcatg acgtcaaatc atgctcatcg tgaaaaagtt ttggagtatt
     5041 tttggaattt ttcaatcaag tgaaagttta tgaaattaat tttcctgctt ttgctttttg
     5101 gggtttcccc tattgtttgt caagatttcg aggacggcgt ttttcttgct aaaatcacaa
     5161 gtattgatga gcacgatgca agaaagatcg gaagaaggtt tgggtttgag gctcagtgga
     5221 aggtgagtag aagttgataa tttgaaagtg gagtagtgtc tatggggttt ttgccttaaa
     5281 tgacagaata cattcccaat ataccaaaca taactgtttc ctactagtcg gccgtacggg
     5341 ccctttcgtc tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg
     5401 gagacggtca cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg
     5461 tcagcgggtg ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta
     5521 ctgagagtgc accatatgcg gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc
     5581 atcaggcggc cttaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat
     5641 aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg
     5701 tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat
     5761 gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat
     5821 tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt
     5881 aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag
     5941 cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa
     6001 agttctgcta tgtggcgcgg tattatcccg tattgacgcc gggcaagagc aactcggtcg
     6061 ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct
     6121 tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac
     6181 tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca
     6241 caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat
     6301 accaaacgac gagcgtgaca ccacgatgcc tgtagcaatg gcaacaacgt tgcgcaaact
     6361 attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc
     6421 ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga
     6481 taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg
     6541 taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg
     6601 aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca
     6661 agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta
     6721 ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca
     6781 ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg
     6841 cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga
     6901 tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa
     6961 tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc
     7021 tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg
     7081 tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac
     7141 ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct
     7201 acagcgtgag cattgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc
     7261 ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg
     7321 gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg
     7381 ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct
     7441 ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga
     7501 taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg
     7561 cagcgagtca gtgagcgagg aagcggaaga gcgcccaata cgcaaaccgc ctctccccgc
     7621 gcgttggccg attcattaat gcagctggca cgacaggttt cccgactgga aagcgggcag
     7681 tgagcgcaac gcaattaatg tgagttagct cactcattag gcaccccagg ctttacactt
     7741 tatgcttccg gctcgtatgt tgtgtggaat tgtgagcgga taacaatt
//
