LOCUS       45952_126               7571 bp DNA     circular SYN 03-FEB-2016
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7571)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Sep 7, 2022 from SnapGene 6.1.1
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..7571
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     source          join(3871..3880,4307..5161)
                     /mol_type="other DNA"
                     /label=source:pPD95_62
                     /organism="pPD95_62"
     primer_bind     6..22
                     /label=M13R_GW_PRIMER
     misc_feature    113..131
                     /label=EV Landmark FWD
     misc_feature    164..168
                     /label=PstI Cut Site
                     /label=PstI Cut Site(1)
     misc_feature    169..2822
                     /label=flp-32
                     /label=flp-32(1)
     misc_feature    169
                     /label=PstI Cut Site
     misc_feature    2750..2767
                     /label=EV flp-32 End FWD
     misc_feature    2812..2821
                     /label=flp-32
     misc_feature    2822
                     /label=XmaI Cut Site
     misc_feature    2927..2968
                     /label=1xNLS
     misc_feature    2928..2947
                     /label=Color FWD
     misc_feature    2969..3004
                     /label=NLS2
     misc_feature    join(3005..3178,3230..3412,3464..3655,3707..3856)
                     /label=ceBFP2
     misc_feature    3386..3405
                     /label=EV ceBFP2 Mid REV
     misc_feature    3626..3628
                     /label=I to A mTagBFP2 Fix
     misc_feature    complement(3857..3862)
                     /label=Linker AvrII Cut Site
     misc_feature    complement(3863..3864)
                     /label=Frame Link
     misc_feature    complement(3865..3870)
                     /label=AgeI Cut Site
     misc_feature    3881..4246
                     /label=H2B
     misc_feature    4028..4047
                     /label=H2B Mid REV
     misc_feature    4247..4252
                     /label=EcoRI Cut Site
     misc_feature    4253..4306
                     /label=Ligated Addition
     misc_feature    4375..5102
                     /label=unc-54 3'UTR
     misc_feature    4385..4403
                     /label=EV unc-54 3'UTR REV
     misc_feature    4853..5005
                     /label=aex-5 Exon1
     primer_bind     complement(5103..5128)
                     /label=Primer D54
     primer_bind     complement(5123..5148)
                     /label=PrimerD858
     misc_feature    5140..5157
                     /label=EV Landmark REV
     primer_bind     complement(5162..5184)
                     /label=3GEX_GW_PRIMER
     misc_feature    5162..5179
                     /label=EV ceBFP2 + H2B GIB REV
     CDS             5770..6136
                     /label=AmpR
     CDS             6137..6429
                     /label=AmpR
                     /label=AmpR(1)
     rep_origin      6527..7209
                     /label=ColE1 origin
     misc_binding    7549..7571
                     /label=LacO
ORIGIN
        1 tcacacagga aacagctatg accatgatta cgccaagctg taagtttaaa catgatctta
       61 ctaactaact attctcattt aaattttcag agcttaaaaa tggctgaaat cactcacaac
      121 gatggatacg ctaacaactt ggaaatgaaa taagcttgca tgcctgcagg aatcgataat
      181 gtgcgaaggg ggacatagtg gtgaggggag ggtgagagag tgtggaggag tagagaaaaa
      241 gagaagtgta acgatggtgc atctttagac caaaatcata aaaggcaagt aacgatatta
      301 aaatatatga tcccgtttgt ctactattgc attttgtatc ttggttctct ctgattttat
      361 cgaaaaattg ttaattaaca aaatatgtgc aaaatatttt cttttacatt ttgatgttgg
      421 aacatttttt ggaaaatttt agacgaccga gatataacct gctaaagtag aaaaatggag
      481 gtacaagttg tataggaaaa ttacggttat ggaaatataa ctagaaattt ttattctcat
      541 ttaaaattca aagagcttac atataaacaa agtaacatga aaaagcaaag aacatggcaa
      601 aacaatcatg agaatgaggt tttaatgctt gcaaggagaa cccgtaaata tacattctac
      661 cctatatatt ttcccatagt tacattctca caaatagcgg gtttcccaac aagataccga
      721 atttttctaa ttagtagtag tgcatgcaat agtatatttc aattgatatt tttgacccta
      781 ataacagatt tttttgtcgg ttttctaatt taaaaatcgt aataataaaa catattcccc
      841 cccccccccc ccaatatata agaactatcg ttccaaaatg aactactccc ataagctgtt
      901 ctcatggtgg ttttttttaa tcaagaagat taacgcaaaa atatagtctt cttccaaatg
      961 attgtcaaaa catttaaggc tcgatcaaac gtcttatacc aataaatatt catataaaat
     1021 tattcctaaa tgaataaacc cctatgattt tggtaaaacc gcaaattgaa aattctcatt
     1081 actttttgaa cctacccaga acttggaaaa atcactgaga tgattcatgt ttttgtttgc
     1141 ttttatcaaa tatgtataga aatcctctaa ccttggttat gtgctcttga gagaatctag
     1201 gatttttttg cctgcaagct tctggttttt ggaacacaaa tgatattctt ttttgcgaaa
     1261 agttcactgt ttgtcaggcg ttcttcactt acttaattta ttttttcaaa taatttcata
     1321 atttaataaa tgttggaatg gaatgcgagt atttctcaat tttgttgtgc ttttttgttc
     1381 ctgatcataa aattctacgg aaataaaatt ggaacggttg ttgttttctt ccaatttttg
     1441 agcgcttgaa agaacacaaa ctataagtcc gaggcgcatc acagagttca agtttttaaa
     1501 aagattttgc atttcagttt tagaccaatt ttgctggttt ccaaaattga ataattagac
     1561 ttatccaatc tgtagtaaaa ttgcatttac ggaaaatatt acaatgcaag gccgtaagta
     1621 tattttaaaa attgagtacg cctgtgggac tttttcaaac atttagtaat tatttatttt
     1681 tttgacttgc tcacagtaac tcatggaggc caaaaccggt tgaaaaaatt tatccatcgt
     1741 aaggatgttc gacaaatttc agcagtttag atcagcaaac aagaatgttt gaatgaaagt
     1801 ttgttggacg tgccaagcaa tgcttagacc taggtaactt tcgtgaccat ttttgaagat
     1861 cacctatttt attttattca ctataatttc cttttcacat actacttcga aattcaaagc
     1921 attatttttc gtgtggactc tgaaccgatc gagaacatta cagaagctta taacacttaa
     1981 gtgctcttaa aataatgttg tttaccttgc agaaaattca tcgaatacct cccaacaagt
     2041 atacacctga aatgtgctcc acaattgtct tgttgcacac aaagttcccc cgtttccaac
     2101 tatcggcaat tcgttgttcc tacctcgtca ttttgtcttt aaattatcgg aacaatggaa
     2161 caaaagaata agtgagaagt gaaaaagaga aagattcgtc ttttttttgc gctgacactg
     2221 ttgaaaatgt atgagtgggg tgtgagttgg cggcgtcgac gaacattgcc aagacaaaga
     2281 aacgttggtc tgtgtggttc tttggtaaaa cattaacttt ttggttagta gatagggtta
     2341 ggtactcaac cacacaccaa gaatgaggag tttctccttt gttcacgttc agatttttga
     2401 tcggcttcgg cttcttcgtc ccgcgccgca attagtaata ttctgttctc gacagcttat
     2461 cccatctaaa acccggaaaa agtcgggctc atcctccgag ccggagactc cgacatcact
     2521 ttttggaaag aaattacgtg ttcaaattca ggttttcgaa atgtaatgcg attgctgctt
     2581 catttgttat tcgattatat cgttccgttc tctggctcaa ttatggaagt gtcattttgg
     2641 taagtaaccc tcccagtact aaacacacac tgtcatccct acgaaagcac gacaaaaaca
     2701 gtgcggatga cacatgtgcc agtggcagct aaagtgggag gggtcttcgg gttgggcaat
     2761 gggaatctga aagcagctcg ccgagtgagt gccacgcccc acgaagcctt cattcttacg
     2821 acccgggatt ggccaaagga cccaaaggta tgtttcgaat gatactaaca taacatagaa
     2881 cattttcagg aggacccttg cttggagggt accgagctca gaaaaaatga ccgctccaaa
     2941 gaagaagcgc aaggttccag tcgagaagat gactgcccca aaaaagaaac gtaaggtccc
     3001 gcggatgtca gagcttatta aggagaatat gcatatgaaa ctgtacatgg aaggaaccgt
     3061 ggacaaccat cactttaagt gcacttcgga gggggaaggc aaaccgtacg agggcaccca
     3121 gaccatgcga atcaaggtgg tcgagggagg cccactgccc ttcgccttcg acattctggt
     3181 aagtttaaac atatatatac taactaaccc tgattattta aattttcagg ctactagctt
     3241 cctgtacggc agcaagactt ttattaatca cacccagggc atcccggact tcttcaaaca
     3301 gtcgttccct gagggcttca catgggagcg tgtgaccaca tacgaagacg gtggcgttct
     3361 caccgctacc caggacacca gcctccagga cggttgcctc atatataatg tggtaagttt
     3421 aaacagttcg gtactaacta accatacata tttaaatttt cagaagatcc ggggagtaaa
     3481 cttcacatcc aatggtccgg ttatgcagaa gaaaacactc ggctgggagg ccttcaccga
     3541 gacgctgtat ccggctgacg gaggcctgga aggtcgaaac gacatggccc tgaagctcgt
     3601 cggcggtagc catctcatcg caaacgctaa gaccacatat agatccaaga aaccggtaag
     3661 tttaaacatg attttactaa ctaactaatc tgatttaaat tttcaggcta agaaccttaa
     3721 aatgccggga gtctactatg ttgactacag actggaacgg atcaaggagg ccaacaacga
     3781 gacctacgtc gagcagcacg aggttgcagt tgccagatac tgcgacctcc cgagcaaact
     3841 gggtcacaag cttaatccta ggataccggt gggtgaagac ccaccaaagc catctgccaa
     3901 gggagccaag aaggccgcca agaccgttac gaagccaaag gacggaaaga agagacgtca
     3961 tgcccgtaag gaatcatact ccgtctacat ctaccgtgtc ctcaagcaag ttcatccaga
     4021 cactggagtt tcctccaaag ccatgtctat catgaactct tttgtcaacg atgtcttcga
     4081 gcgtattgct gctgaagcat cccgtcttgc tcactacaac aagcgttcca caatctcatc
     4141 ccgcgaaatt cagaccgctg tccgtctgat ccttccagga gagcttgcca agcacgccgt
     4201 gtctgaggga accaaggccg ttaccaagta cacttccagc aagtaggaat tccaactgag
     4261 cgccggtcgc taccattacc aacttgtctg gtgtcaaaaa taataggggc cgctgtcatc
     4321 agagtaagtt taaactgagt tctactaact aacgagtaat atttaaattt tcagcatctc
     4381 gcgcccgtgc ctctgacttc taagtccaat tactcttcaa catccctaca tgctctttct
     4441 ccctgtgctc ccacccccta tttttgttat tatcaaaaaa cttctcttaa tttctttgtt
     4501 ttttagcttc ttttaagtca cctctaacaa tgaaattgtg tagattcaaa aatagaatta
     4561 attcgtaata aaaagtcgaa aaaaattgtg ctccctcccc ccattaataa taattctatc
     4621 ccaaaatcta cacaatgttc tgtgtacact tcttatgttt tttacttctg ataaattttt
     4681 ttgaaacatc atagaaaaaa ccgcacacaa aataccttat catatgttac gtttcagttt
     4741 atgaccgcaa tttttatttc ttcgcacgtc tgggcctctc atgacgtcaa atcatgctca
     4801 tcgtgaaaaa gttttggagt atttttggaa tttttcaatc aagtgaaagt ttatgaaatt
     4861 aattttcctg cttttgcttt ttggggtttc ccctattgtt tgtcaagatt tcgaggacgg
     4921 cgtttttctt gctaaaatca caagtattga tgagcacgat gcaagaaaga tcggaagaag
     4981 gtttgggttt gaggctcagt ggaaggtgag tagaagttga taatttgaaa gtggagtagt
     5041 gtctatgggg tttttgcctt aaatgacaga atacattccc aatataccaa acataactgt
     5101 ttcctactag tcggccgtac gggccctttc gtctcgcgcg tttcggtgat gacggtgaaa
     5161 acctctgaca catgcagctc ccggagacgg tcacagcttg tctgtaagcg gatgccggga
     5221 gcagacaagc ccgtcagggc gcgtcagcgg gtgttggcgg gtgtcggggc tggcttaact
     5281 atgcggcatc agagcagatt gtactgagag tgcaccatat gcggtgtgaa ataccgcaca
     5341 gatgcgtaag gagaaaatac cgcatcaggc ggccttaagg gcctcgtgat acgcctattt
     5401 ttataggtta atgtcatgat aataatggtt tcttagacgt caggtggcac ttttcgggga
     5461 aatgtgcgcg gaacccctat ttgtttattt ttctaaatac attcaaatat gtatccgctc
     5521 atgagacaat aaccctgata aatgcttcaa taatattgaa aaaggaagag tatgagtatt
     5581 caacatttcc gtgtcgccct tattcccttt tttgcggcat tttgccttcc tgtttttgct
     5641 cacccagaaa cgctggtgaa agtaaaagat gctgaagatc agttgggtgc acgagtgggt
     5701 tacatcgaac tggatctcaa cagcggtaag atccttgaga gttttcgccc cgaagaacgt
     5761 tttccaatga tgagcacttt taaagttctg ctatgtggcg cggtattatc ccgtattgac
     5821 gccgggcaag agcaactcgg tcgccgcata cactattctc agaatgactt ggttgagtac
     5881 tcaccagtca cagaaaagca tcttacggat ggcatgacag taagagaatt atgcagtgct
     5941 gccataacca tgagtgataa cactgcggcc aacttacttc tgacaacgat cggaggaccg
     6001 aaggagctaa ccgctttttt gcacaacatg ggggatcatg taactcgcct tgatcgttgg
     6061 gaaccggagc tgaatgaagc cataccaaac gacgagcgtg acaccacgat gcctgtagca
     6121 atggcaacaa cgttgcgcaa actattaact ggcgaactac ttactctagc ttcccggcaa
     6181 caattaatag actggatgga ggcggataaa gttgcaggac cacttctgcg ctcggccctt
     6241 ccggctggct ggtttattgc tgataaatct ggagccggtg agcgtgggtc tcgcggtatc
     6301 attgcagcac tggggccaga tggtaagccc tcccgtatcg tagttatcta cacgacgggg
     6361 agtcaggcaa ctatggatga acgaaataga cagatcgctg agataggtgc ctcactgatt
     6421 aagcattggt aactgtcaga ccaagtttac tcatatatac tttagattga tttaaaactt
     6481 catttttaat ttaaaaggat ctaggtgaag atcctttttg ataatctcat gaccaaaatc
     6541 ccttaacgtg agttttcgtt ccactgagcg tcagaccccg tagaaaagat caaaggatct
     6601 tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta
     6661 ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc tttttccgaa ggtaactggc
     6721 ttcagcagag cgcagatacc aaatactgtc cttctagtgt agccgtagtt aggccaccac
     6781 ttcaagaact ctgtagcacc gcctacatac ctcgctctgc taatcctgtt accagtggct
     6841 gctgccagtg gcgataagtc gtgtcttacc gggttggact caagacgata gttaccggat
     6901 aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac agcccagctt ggagcgaacg
     6961 acctacaccg aactgagata cctacagcgt gagcattgag aaagcgccac gcttcccgaa
     7021 gggagaaagg cggacaggta tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg
     7081 gagcttccag ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg ccacctctga
     7141 cttgagcgtc gatttttgtg atgctcgtca ggggggcgga gcctatggaa aaacgccagc
     7201 aacgcggcct ttttacggtt cctggccttt tgctggcctt ttgctcacat gttctttcct
     7261 gcgttatccc ctgattctgt ggataaccgt attaccgcct ttgagtgagc tgataccgct
     7321 cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg aggaagcgga agagcgccca
     7381 atacgcaaac cgcctctccc cgcgcgttgg ccgattcatt aatgcagctg gcacgacagg
     7441 tttcccgact ggaaagcggg cagtgagcgc aacgcaatta atgtgagtta gctcactcat
     7501 taggcacccc aggctttaca ctttatgctt ccggctcgta tgttgtgtgg aattgtgagc
     7561 ggataacaat t
//
