LOCUS       45952_132               5803 bp DNA     circular SYN 20-FEB-2017
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5803)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Sep 7, 2022 from SnapGene 6.1.1
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..5803
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     source          2539..3393
                     /mol_type="other DNA"
                     /label=source:pPD95_62
                     /organism="pPD95_62"
     primer_bind     6..22
                     /label=M13R_GW_PRIMER
     misc_feature    113..131
                     /label=EV Landmark FWD
     misc_feature    170..1047
                     /label=gcy-25
     misc_feature    170..309
                     /label=Y105C5B.1
     misc_feature    170..309
                     /label=Y105C5B.1 exons
     misc_feature    948..966
                     /label=EV gcy-25 End FWD
     misc_feature    1021..1047
                     /label=gcy-25 partial
     misc_feature    1153..1194
                     /label=1xNLS
     misc_feature    1154..1173
                     /label=Color FWD
     misc_feature    1195..1230
                     /label=NLS2
     misc_feature    1225..1230
                     /label=SacII Cut Site
     misc_feature    1281..1286
                     /label=BssHII Cut Site
     misc_feature    join(1288..1497,1549..1827,1879..2088)
                     /label=CyOFP1
     misc_feature    1323..1340
                     /label=EV CyOFP Start REV
     misc_feature    1596..1614
                     /label=EV CYOFP Mid2 FWD
     misc_feature    1663..1681
                     /label=EV CyOFP1 Mid FWD
     misc_feature    1715..1732
                     /label=EV CyOFP1 Mid REV
     misc_feature    1773..1791
                     /label=Ev CYOFP Mid3 FWD
     misc_feature    1903..1921
                     /label=EV CYOFP Mid3 REV
     misc_feature    1908..1926
                     /label=EV CyOFP Mid2 REV
     misc_feature    2089..2094
                     /label=AvrII Cut Site
     misc_feature    complement(2095..2096)
                     /label=Frame Link
     misc_feature    complement(2097..2102)
                     /label=AgeI Cut Site
     misc_feature    2113..2478
                     /label=H2B
     misc_feature    2260..2279
                     /label=H2B Mid REV
     misc_feature    2479..2484
                     /label=EcoRI Cut Site
     misc_feature    2485..2538
                     /label=Ligated Addition
     misc_feature    2607..3334
                     /label=unc-54 3'UTR
     misc_feature    2617..2635
                     /label=EV unc-54 3'UTR REV
     misc_feature    3085..3237
                     /label=aex-5 Exon1
     primer_bind     complement(3335..3360)
                     /label=Primer D54
     primer_bind     complement(3355..3380)
                     /label=PrimerD858
     misc_feature    3372..3389
                     /label=EV Landmark REV
     primer_bind     complement(3394..3416)
                     /label=3GEX_GW_PRIMER
     misc_feature    3394..3411
                     /label=EV ceBFP2 + H2B GIB REV
     CDS             4002..4368
                     /label=AmpR
     CDS             4369..4661
                     /label=AmpR
                     /label=AmpR(1)
     rep_origin      4759..5441
                     /label=ColE1 origin
     misc_binding    5781..5803
                     /label=LacO
ORIGIN
        1 tcacacagga aacagctatg accatgatta cgccaagctg taagtttaaa catgatctta
       61 ctaactaact attctcattt aaattttcag agcttaaaaa tggctgaaat cactcacaac
      121 gatggatacg ctaacaactt ggaaatgaaa taagcttgca tgcctgcagt gtttgaccac
      181 gtaactattt cactaacaac catattccca attatcattt caatttctta tatcaaaaca
      241 agattcgatt tcaaaactgt aattttgtca gtttttcgag cgaaccggtc ggaggtgtcg
      301 ccaacctgat tttgtggttt ttttgaaata aaattaccgt ttcacaaatt attgattcac
      361 taaatctcca ctttatatcg cagaaattga gaaaagcagc ggacagacgt ctaagatcgc
      421 gacgggtcaa ttattcttct gtgtatctta caatggttaa ataatcattt aatgtaaaaa
      481 attcgggatt atatcattct ggcacaaata attaaaatct ttttttttcg tgggacgctg
      541 gactaattga aaacttttat aagtcgttgt ttttctggcc tatgtgaaac agtgaatttt
      601 aggaaagttt tcgtttgtga aaactcataa actaaaacat tctcatcaat taaatttcac
      661 aaacaaagac caaacttcac ccaataaatg agcaacatac ttgaccgaat aaatgagcaa
      721 catgaaactg taaaaatttc ttgaacattt tttcaaaaaa ttgcaatatt ttatcaagtt
      781 ttgtatagtt ccaatcagac tacatagata aagtttcaaa aagttcacgt agtagtacta
      841 aaatcactga ataatacaga aatccaagat gaaaatgcaa aaaatttcgg cggaaaaagt
      901 cacgtttttc taatcgtcta ctacaccttt tgaataatta attcctttcg atggctcaca
      961 agatggattg ataagtgaaa agaagcataa caacacatca aaaattcatt ctcgatctgc
     1021 agttgtgagc tccgagagca ttcggagccc gggattggcc aaaggaccca aaggtatgtt
     1081 tcgaatgata ctaacataac atagaacatt ttcaggagga cccttgcttg gagggtaccg
     1141 agctcagaaa aaatgaccgc tccaaagaag aagcgcaagg ttccagtcga gaagatgact
     1201 gccccaaaaa agaaacgtaa ggtcccgcgg gtaagtttaa acagttcggt actaactaac
     1261 catacatatt taaattttca gcgcgctgtg tcaaaaggag aagagcttat taaggagaat
     1321 atgcgttcca agctctacct cgagggatcc gtcaacggac accaattcaa gtgcacccac
     1381 gagggagagg gaaagccata cgagggaaag caaaccaacc gtatcaaggt cgtcgaggga
     1441 ggaccactcc cattcgcctt cgacatcctc gccacccact tcatgtacgg atccaaggta
     1501 agtttaaaca tatatatact aactaaccct gattatttaa attttcaggt cttcatcaag
     1561 tacccagccg acctcccaga ctacttcaag caatccttcc cagagggatt cacctgggag
     1621 cgtgtcatgg tcttcgagga cggaggagtc ctcaccgcca cccaagacac ctccctccaa
     1681 gacggagagc tcatctacaa cgtcaaggtc cgtggagtca acttcccagc caacggacca
     1741 gtcatgcaaa agaagaccct cggatgggag ccatccaccg agaccatgta cccagccgac
     1801 ggaggactcg agggacgttg cgacaaggta agtttaaaca tgattttact aactaactaa
     1861 tctgatttaa attttcaggc cctcaagctc gtcggaggag gacacctcca cgtcaacttc
     1921 aagaccacct acaagtccaa gaagccagtc aagatgccag gagtccacta cgtcgaccgt
     1981 cgtctcgagc gtatcaagga ggccgacaac gagacctacg tcgagcaata cgagcacgcc
     2041 gtcgcccgtt actccaacct cggaggagga atggacgagc tctacaagcc taggataccg
     2101 gtgggtgaag acccaccaaa gccatctgcc aagggagcca agaaggccgc caagaccgtt
     2161 acgaagccaa aggacggaaa gaagagacgt catgcccgta aggaatcata ctccgtctac
     2221 atctaccgtg tcctcaagca agttcatcca gacactggag tttcctccaa agccatgtct
     2281 atcatgaact cttttgtcaa cgatgtcttc gagcgtattg ctgctgaagc atcccgtctt
     2341 gctcactaca acaagcgttc cacaatctca tcccgcgaaa ttcagaccgc tgtccgtctg
     2401 atccttccag gagagcttgc caagcacgcc gtgtctgagg gaaccaaggc cgttaccaag
     2461 tacacttcca gcaagtagga attccaactg agcgccggtc gctaccatta ccaacttgtc
     2521 tggtgtcaaa aataataggg gccgctgtca tcagagtaag tttaaactga gttctactaa
     2581 ctaacgagta atatttaaat tttcagcatc tcgcgcccgt gcctctgact tctaagtcca
     2641 attactcttc aacatcccta catgctcttt ctccctgtgc tcccaccccc tatttttgtt
     2701 attatcaaaa aacttctctt aatttctttg ttttttagct tcttttaagt cacctctaac
     2761 aatgaaattg tgtagattca aaaatagaat taattcgtaa taaaaagtcg aaaaaaattg
     2821 tgctccctcc ccccattaat aataattcta tcccaaaatc tacacaatgt tctgtgtaca
     2881 cttcttatgt tttttacttc tgataaattt ttttgaaaca tcatagaaaa aaccgcacac
     2941 aaaatacctt atcatatgtt acgtttcagt ttatgaccgc aatttttatt tcttcgcacg
     3001 tctgggcctc tcatgacgtc aaatcatgct catcgtgaaa aagttttgga gtatttttgg
     3061 aatttttcaa tcaagtgaaa gtttatgaaa ttaattttcc tgcttttgct ttttggggtt
     3121 tcccctattg tttgtcaaga tttcgaggac ggcgtttttc ttgctaaaat cacaagtatt
     3181 gatgagcacg atgcaagaaa gatcggaaga aggtttgggt ttgaggctca gtggaaggtg
     3241 agtagaagtt gataatttga aagtggagta gtgtctatgg ggtttttgcc ttaaatgaca
     3301 gaatacattc ccaatatacc aaacataact gtttcctact agtcggccgt acgggccctt
     3361 tcgtctcgcg cgtttcggtg atgacggtga aaacctctga cacatgcagc tcccggagac
     3421 ggtcacagct tgtctgtaag cggatgccgg gagcagacaa gcccgtcagg gcgcgtcagc
     3481 gggtgttggc gggtgtcggg gctggcttaa ctatgcggca tcagagcaga ttgtactgag
     3541 agtgcaccat atgcggtgtg aaataccgca cagatgcgta aggagaaaat accgcatcag
     3601 gcggccttaa gggcctcgtg atacgcctat ttttataggt taatgtcatg ataataatgg
     3661 tttcttagac gtcaggtggc acttttcggg gaaatgtgcg cggaacccct atttgtttat
     3721 ttttctaaat acattcaaat atgtatccgc tcatgagaca ataaccctga taaatgcttc
     3781 aataatattg aaaaaggaag agtatgagta ttcaacattt ccgtgtcgcc cttattccct
     3841 tttttgcggc attttgcctt cctgtttttg ctcacccaga aacgctggtg aaagtaaaag
     3901 atgctgaaga tcagttgggt gcacgagtgg gttacatcga actggatctc aacagcggta
     3961 agatccttga gagttttcgc cccgaagaac gttttccaat gatgagcact tttaaagttc
     4021 tgctatgtgg cgcggtatta tcccgtattg acgccgggca agagcaactc ggtcgccgca
     4081 tacactattc tcagaatgac ttggttgagt actcaccagt cacagaaaag catcttacgg
     4141 atggcatgac agtaagagaa ttatgcagtg ctgccataac catgagtgat aacactgcgg
     4201 ccaacttact tctgacaacg atcggaggac cgaaggagct aaccgctttt ttgcacaaca
     4261 tgggggatca tgtaactcgc cttgatcgtt gggaaccgga gctgaatgaa gccataccaa
     4321 acgacgagcg tgacaccacg atgcctgtag caatggcaac aacgttgcgc aaactattaa
     4381 ctggcgaact acttactcta gcttcccggc aacaattaat agactggatg gaggcggata
     4441 aagttgcagg accacttctg cgctcggccc ttccggctgg ctggtttatt gctgataaat
     4501 ctggagccgg tgagcgtggg tctcgcggta tcattgcagc actggggcca gatggtaagc
     4561 cctcccgtat cgtagttatc tacacgacgg ggagtcaggc aactatggat gaacgaaata
     4621 gacagatcgc tgagataggt gcctcactga ttaagcattg gtaactgtca gaccaagttt
     4681 actcatatat actttagatt gatttaaaac ttcattttta atttaaaagg atctaggtga
     4741 agatcctttt tgataatctc atgaccaaaa tcccttaacg tgagttttcg ttccactgag
     4801 cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa
     4861 tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg ccggatcaag
     4921 agctaccaac tctttttccg aaggtaactg gcttcagcag agcgcagata ccaaatactg
     4981 tccttctagt gtagccgtag ttaggccacc acttcaagaa ctctgtagca ccgcctacat
     5041 acctcgctct gctaatcctg ttaccagtgg ctgctgccag tggcgataag tcgtgtctta
     5101 ccgggttgga ctcaagacga tagttaccgg ataaggcgca gcggtcgggc tgaacggggg
     5161 gttcgtgcac acagcccagc ttggagcgaa cgacctacac cgaactgaga tacctacagc
     5221 gtgagcattg agaaagcgcc acgcttcccg aagggagaaa ggcggacagg tatccggtaa
     5281 gcggcagggt cggaacagga gagcgcacga gggagcttcc agggggaaac gcctggtatc
     5341 tttatagtcc tgtcgggttt cgccacctct gacttgagcg tcgatttttg tgatgctcgt
     5401 caggggggcg gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct
     5461 tttgctggcc ttttgctcac atgttctttc ctgcgttatc ccctgattct gtggataacc
     5521 gtattaccgc ctttgagtga gctgataccg ctcgccgcag ccgaacgacc gagcgcagcg
     5581 agtcagtgag cgaggaagcg gaagagcgcc caatacgcaa accgcctctc cccgcgcgtt
     5641 ggccgattca ttaatgcagc tggcacgaca ggtttcccga ctggaaagcg ggcagtgagc
     5701 gcaacgcaat taatgtgagt tagctcactc attaggcacc ccaggcttta cactttatgc
     5761 ttccggctcg tatgttgtgt ggaattgtga gcggataaca att
//
