LOCUS       45952_146               7159 bp DNA     circular SYN 15-MAY-2017
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7159)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Sep 7, 2022 from SnapGene 6.1.1
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..7159
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     6..22
                     /label=M13R_GW_PRIMER
     misc_feature    113..131
                     /label=EV Landmark FWD
     misc_feature    168..2695
                     /label=gpa-11
     misc_feature    168..327
                     /label=C16A11.2
     misc_feature    168..193
                     /label=C16A11.2 exons
     misc_feature    2215..2232
                     /label=EV gpa-11 End FWD
     misc_feature    2695
                     /label=gpa-11
                     /label=gpa-11(1)
     misc_feature    2695
                     /label=gpa-11 exons
     misc_feature    2722..2763
                     /label=artificial intron
     misc_feature    2778..2783
                     /label=KpnI Cut Site
     primer_bind     2784..2801
                     /label=M13F-41_GW_PRIMER
     misc_feature    2800..2805
                     /label=MluI Cut Site
                     /label=MluI Cut Site(1)
     misc_feature    2806..2811
                     /label=KpnI Cut Site
                     /label=KpnI Cut Site(1)
     misc_feature    2825..2905
                     /label=2xNLS
     misc_feature    2944..2949
                     /label=MluI Cut Site
     misc_feature    2946..2966
                     /label=EV NLS3 intron REV
     misc_feature    2951..2953
                     /label=ORF ATG
     misc_feature    2957..3007
                     /label=intron
     misc_feature    3007..3012
                     /label=BssHII Cut Site
     CDS             join(3080..3103,3106..3142,3144,3146..3241,3269..3274,
                     3326..3500,3503..3640,3698..3847)
                     /codon_start=1
                     /label=mCardinal
                     /translation="MNHHFKCTRRGGQALRGHPDQGLRWWREAPCRSHSTSWPPALCTG
                     ARPSSTTPPSLRASHGRESPHTKTGACLPLPRTPASRTAA*STTSSSEG*TSHPTAL*C
                     RRKHSAGRPTETLYPADGGLEGRCDMALKLVGGGHLHCNLKTTYRSKKPAKNLKMVYFV
                     DRRLERIKEADNETYVEQHEVAVARYCDLPSKLGHKLNGMDELYK"
     misc_feature    3206..3208
                     /label=Same as mBeRFP Start
     misc_feature    3209..3226
                     /label=EV mNeptune2.5 Mid FWD
     misc_feature    3275..3325
                     /label=intron 2
     misc_feature    3320..3337
                     /label=EV mBeRFP/mNep2.5 intron3 Mid FWD
     misc_feature    3326..3332
                     /label=Bsu36I Cut Site
     misc_feature    3332..3349
                     /label=EV mNeptune2.5 Mid REV
     misc_feature    3347..3349
                     /label=Same as mBeRFP End
     misc_feature    3613..3632
                     /label=EV mCardinal Mid REV
     misc_feature    3634..3656
                     /label=EV mNeptune2.5 intron MID REV
     misc_feature    3647..3697
                     /label=intron 3
     misc_feature    3698..3703
                     /label=AccI Cut Site
     misc_feature    3821..3823
                     /label=Last mChOpti Difference
     misc_feature    3848..4670
                     /label=unc-54 3' UTR
     misc_feature    3862..3883
                     /label=EV unc-54 3'UTR GIB REV
     misc_feature    3973..3991
                     /label=EV unc-54 3'UTR REV
     misc_feature    4728..4745
                     /label=EV Landmark REV
     primer_bind     complement(4750..4772)
                     /label=3GEX_GW_PRIMER
     CDS             5358..6017
                     /label=AmpR
     rep_origin      6115..6797
                     /label=ColE1 origin
     misc_binding    7137..7159
                     /label=LacO
ORIGIN
        1 tcacacagga aacagctatg accatgatta cgccaagctg taagtttaaa catgatctta
       61 ctaactaact attctcattt aaattttcag agcttaaaaa tggctgaaat cactcacaac
      121 gatggatacg ctaacaactt ggaaatgaaa taagcttgca tgcctgcagg acgaagaagc
      181 cgaagaatct taaaataatt tttattcaat ttttaaactt ttcaatttga tcccgccgcc
      241 ttatagattc acttttattt cccaaatctt gttgttgttt ttttttaatt taaaatgctt
      301 cattttccag taaattcgtg caaaattcaa gaatcttgac aaaatgacgt catcatcact
      361 caacacgcca cttttcacac taatttcttt gacttcggga aacacctgga gagcacaaaa
      421 cttgctctct ctttttctct cgatctatta atattcgagg gcagctagct gagatttttc
      481 caatttttta tatttgatac gaactttttt attcgaaaat tcgctaaaat tgccattgtg
      541 ggtctcgcca cgagcgaaaa ttttcaggta cagtactctt aaaaagaaat ctctggggta
      601 cggtagctga gaagtttctt cagaaatccc ccaaaattac aattttgggt cccgccacga
      661 gtgaaacttt tcaactacag taccccagag agtacccccc ccccctctgt agctgaaaag
      721 ttttctcagt tttttttcaa agatttttaa aactaatttt tgtgaaacat tttcaacaaa
      781 aaacatttaa gaatccgaaa aaagagtttt aaaaatttaa aagtacatct cagagggtac
      841 tgtacctgga aagttttgtt cgtgccagga cccaaagcca gtttttttgc gctctactgc
      901 gaaaatttca aaattattgt gaaatttgtt tcgaaatcgg aaagttttca aaacaaaaaa
      961 ctggattttc aaaaaattcg tcgaaaaatg ggccctggta cgagcgaaac ttttcagtta
     1021 cagtacccct gagattttat tttttttttc gaattttttc tactttttca ctgattaatc
     1081 acaacaataa ataaatttga atcacaaaaa aaaagggaaa atactagaat aattgagcaa
     1141 aatgggtacg gtagctgaac aatttcgctc gtggcaggac ccaaaaagac cgaattttct
     1201 ctttttatgg cgaaattctt ctctccccct ctctaatact aaagatacag cacacacacg
     1261 tacttaattg cggttatttg gtgctctgga aaattgttag gtggctcata taattatagc
     1321 caaccccctt ccccacccgc tcttcgtctc tcaaaaccct ctgttttaga tgaattttaa
     1381 gctatgagtt atgccgtttt ttgttgcgtt ttcttgtaat tttgggtctc acagcgaaaa
     1441 aaaagtgctc tattgagaaa attgcgaaat ttactaaaaa aaaaagtaga acttttccac
     1501 aagaaagcat tttttcgcaa aaagttttaa aaagttctcg aggccacttg ctgtgtgacc
     1561 ttgaaaagag aaaatgcggc cagtctcaat taaaaattcc aaaaaactat ttttttgggt
     1621 cctggcacga ccaaaacttt tttagctaca gtacccccaa aatgtattta tttttttgca
     1681 tttccacgag tttatttaat ttttttttcc acttttttac tggtaaaatt tgaatatttg
     1741 aatatttttc gaactgagaa ctactgtacc tgaaaatttt cgctcgtgtg aggagccaag
     1801 atggtaatta taactgaaat attgcactat ttcgcaagta ggtaatatta tattattata
     1861 tattggatga aaagaagtca gaaaaaaaag ttgtcctctt ctcacatgct tctaattttc
     1921 atctgctttt tttgcctttt ttcatttttt tcacagattt tcaaattttt ctcccctttt
     1981 tctcaaaact tccagttttc tttgtgaatt ttcgaatgac ttttttgaat aaaaaattgg
     2041 aaaaaaaaaa tcaattttcg ccgaaaaaat cgaaacttgt ttcattgagc tctgacacac
     2101 tctaatgagc tcagctcagg tgctcgaaag agtatatttg cgaaaaaaaa ctaaatgtct
     2161 ctcacaagat tttttttcga attttacaga aatatgtttc ggtggctgcg taaaaatggt
     2221 ggccgaggtt tctttttttc gagtggccta atttttattt ttatgtttta attctcggcc
     2281 acgaaatttg ggaaaactca gaaattcaag ttctgagaat tctaaaaaaa ttaaaaaacc
     2341 cccaaaattt cgatctcaaa ttgttttttt cagagcattt tttaccgccc cgtaaaactg
     2401 ctctaaaaaa tttttttccg caagtttttt tgctaattct aaacaaaggt tgtagtagga
     2461 aaaataccct cgaaatttag gctctttttt ggaaaactcc gaaattgata aaattgctaa
     2521 aaaaattgct tttggtttta ccgaaatttt ttgaattttc actgaaattt gcgttttctt
     2581 tttggttata ctttaatatc caaaaatgtt ttagtgagtt tttttttcaa gttaaaaaaa
     2641 aatttgtaac ctcatacata tatccccctg aaacttctcc attttcagcc aaaaacccgg
     2701 gattggccaa aggacccaaa ggtatgtttc gaatgatact aacataacat agaacatttt
     2761 caggaggacc cttggagggt accggttttc ccagtcacga cgcgtggtac cgagctcaga
     2821 aaaaatgacc gctccaaaga agaaacgcaa agtaccgagc tcagaaaaaa tgacggcacc
     2881 taaaaagaaa cgcaaagtac cggtcgccga gaagacagcc ccaaaaaaga agcgtaaagt
     2941 tccacgcgtc atgggagtaa gtttaaacag ttcggtacta actaaccata catatttaaa
     3001 ttttcagcgc gctgtctcca agggagagga gctcatcaag gagaacatgc acaccaagct
     3061 ctacatggag ggaaccgtca acaaccacca cttcaagtgc acccacgaag gggagggcaa
     3121 gccctacgag ggcacccaga ccaacaggat taaggtggtg gagggaggcc ccctgccgtt
     3181 cgcattcgac atcctggcca cctgctttat gtacgggagc aagaccttca tcaaccacac
     3241 ccaaggaatc ccagacttct tcaagcaatc cttcgtaagt ttaaacatga ttttactaac
     3301 taactaatct gatttaaatt ttcagcctga gggcttcaca tgggagagag tcaccacata
     3361 cgaagacggg ggcgtgctta ccgttaccca ggacaccagc ctccaggacg gctgcttgat
     3421 ctacaacgtc aagctcagag gggtgaactt cccatccaac ggccctgtga tgcagaagaa
     3481 aacactcggc tgggaggcca gtaccgagac cctgtacccc gctgacggcg gcctggaagg
     3541 cagatgcgac atggccctga agctcgtggg cgggggccac ctgcactgca acctgaagac
     3601 cacatacaga tccaagaaac ccgctaagaa cctcaagatg ccaggagtaa gtttaaacat
     3661 atatatacta actaaccctg attatttaaa ttttcaggtc tactttgtgg accgcagact
     3721 ggaaagaatc aaggaggccg acaatgagac ctacgtcgag cagcacgagg tggctgtggc
     3781 cagatactgc gacctcccta gcaaactggg gcacaaactt aatggcatgg acgagctgta
     3841 caagtaagcg ccggtcgcta ccattaccaa cttgtctggt gtcaaaaata ataggggccg
     3901 ctgtcatcag agtaagttta aactgagttc tactaactaa cgagtaatat ttaaattttc
     3961 agcatctcgc gcccgtgcct ctgacttcta agtccaatta ctcttcaaca tccctacatg
     4021 ctctttctcc ctgtgctccc accccctatt tttgttatta tcaaaaaact tctcttaatt
     4081 tctttgtttt ttagcttctt ttaagtcacc tctaacaatg aaattgtgta gattcaaaaa
     4141 tagaattaat tcgtaataaa aagtcgaaaa aaattgtgct ccctcccccc attaataata
     4201 attctatccc aaaatctaca caatgttctg tgtacacttc ttatgttttt tacttctgat
     4261 aaattttttt gaaacatcat agaaaaaacc gcacacaaaa taccttatca tatgttacgt
     4321 ttcagtttat gaccgcaatt tttatttctt cgcacgtctg ggcctctcat gacgtcaaat
     4381 catgctcatc gtgaaaaagt tttggagtat ttttggaatt tttcaatcaa gtgaaagttt
     4441 atgaaattaa ttttcctgct tttgcttttt ggggtttccc ctattgtttg tcaagatttc
     4501 gaggacggcg tttttcttgc taaaatcaca agtattgatg agcacgatgc aagaaagatc
     4561 ggaagaaggt ttgggtttga ggctcagtgg aaggtgagta gaagttgata atttgaaagt
     4621 ggagtagtgt ctatggggtt tttgccttaa atgacagaat acattcccaa tataccaaac
     4681 ataactgttt cctactagtc ggccgtacgg gccctttcgt ctcgcgcgtt tcggtgatga
     4741 cggtgaaaac ctctgacaca tgcagctccc ggagacggtc acagcttgtc tgtaagcgga
     4801 tgccgggagc agacaagccc gtcagggcgc gtcagcgggt gttggcgggt gtcggggctg
     4861 gcttaactat gcggcatcag agcagattgt actgagagtg caccatatgc ggtgtgaaat
     4921 accgcacaga tgcgtaagga gaaaataccg catcaggcgg ccttaagggc ctcgtgatac
     4981 gcctattttt ataggttaat gtcatgataa taatggtttc ttagacgtca ggtggcactt
     5041 ttcggggaaa tgtgcgcgga acccctattt gtttattttt ctaaatacat tcaaatatgt
     5101 atccgctcat gagacaataa ccctgataaa tgcttcaata atattgaaaa aggaagagta
     5161 tgagtattca acatttccgt gtcgccctta ttcccttttt tgcggcattt tgccttcctg
     5221 tttttgctca cccagaaacg ctggtgaaag taaaagatgc tgaagatcag ttgggtgcac
     5281 gagtgggtta catcgaactg gatctcaaca gcggtaagat ccttgagagt tttcgccccg
     5341 aagaacgttt tccaatgatg agcactttta aagttctgct atgtggcgcg gtattatccc
     5401 gtattgacgc cgggcaagag caactcggtc gccgcataca ctattctcag aatgacttgg
     5461 ttgagtactc accagtcaca gaaaagcatc ttacggatgg catgacagta agagaattat
     5521 gcagtgctgc cataaccatg agtgataaca ctgcggccaa cttacttctg acaacgatcg
     5581 gaggaccgaa ggagctaacc gcttttttgc acaacatggg ggatcatgta actcgccttg
     5641 atcgttggga accggagctg aatgaagcca taccaaacga cgagcgtgac accacgatgc
     5701 ctgtagcaat ggcaacaacg ttgcgcaaac tattaactgg cgaactactt actctagctt
     5761 cccggcaaca attaatagac tggatggagg cggataaagt tgcaggacca cttctgcgct
     5821 cggcccttcc ggctggctgg tttattgctg ataaatctgg agccggtgag cgtgggtctc
     5881 gcggtatcat tgcagcactg gggccagatg gtaagccctc ccgtatcgta gttatctaca
     5941 cgacggggag tcaggcaact atggatgaac gaaatagaca gatcgctgag ataggtgcct
     6001 cactgattaa gcattggtaa ctgtcagacc aagtttactc atatatactt tagattgatt
     6061 taaaacttca tttttaattt aaaaggatct aggtgaagat cctttttgat aatctcatga
     6121 ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc agaccccgta gaaaagatca
     6181 aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac
     6241 caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg
     6301 taactggctt cagcagagcg cagataccaa atactgtcct tctagtgtag ccgtagttag
     6361 gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac
     6421 cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt
     6481 taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg
     6541 agcgaacgac ctacaccgaa ctgagatacc tacagcgtga gcattgagaa agcgccacgc
     6601 ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc
     6661 gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc
     6721 acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa
     6781 acgccagcaa cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt
     6841 tctttcctgc gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg
     6901 ataccgctcg ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag
     6961 agcgcccaat acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgcagctggc
     7021 acgacaggtt tcccgactgg aaagcgggca gtgagcgcaa cgcaattaat gtgagttagc
     7081 tcactcatta ggcaccccag gctttacact ttatgcttcc ggctcgtatg ttgtgtggaa
     7141 ttgtgagcgg ataacaatt
//
